Cell Culture

Syringe Filters

<h2 dir="ltr">What are Syringe Filters?</h2>
 Syringe filters are self-contained, disposable filtration units comprising a polypropylene (PP) housing enclosing a flat-sheet membrane, with a female Luer lock inlet and a male Luer lock outlet. They are attached to a standard syringe, the sample is drawn up, and pushed through the membrane by manual plunger pressure. Standard disc diameters are 4 mm, 13 mm, 25 mm, and 33 mm; membrane pore sizes are 0.22 &micro;m (sterilizing grade) and 0.45 &micro;m (clarification grade), with 0.10 &micro;m available for mycoplasma removal. Effective filtration areas: 13 mm filter &asymp; 0.92 cm&sup2;; 25 mm filter &asymp; 2.98 cm&sup2;. Globe Scientific syringe filters, a confirmed MBP brand, are available in PP housing with PES, PVDF, CA (cellulose acetate), MCE, and nylon membranes in 13 mm and 30 mm diameters, sterile (individually wrapped) and non-sterile (bulk), 0.22 &micro;m and 0.45 &micro;m. Choose syringe filters for volumes of 1&ndash;150 mL; choose vacuum bottle-top filters for volumes above 150 mL.
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<h2 dir="ltr">What you will find:</h2>
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PES Syringe Filters for high-flow aqueous filtration
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PVDF Syringe Filters for low protein binding
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Nylon Syringe Filters for organic solvent compatibility
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0.22&mu;m Syringe Filters for absolute sterilization
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0.45&mu;m Syringe Filters for clarification and pre-filtration
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PTFE Syringe Filters for aggressive chemical resistance
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Hydrophilic Syringe Filters for versatile liquid handling
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Sterile Individually Blistered Filters for aseptic security
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<h2 dir="ltr"> How to Choose Syringe Filters</h2>
 Diameter Selection by Sample Volume 13 mm syringe filters (effective area 0.92 cm&sup2;) handle up to 10&ndash;15 mL per filter before flow resistance becomes impractical. Use with 1&ndash;10 mL syringes. 25 mm syringe filters (2.98 cm&sup2;) handle 10&ndash;100 mL per filter. Use with 10&ndash;60 mL syringes. 33 mm syringe filters (approximately 5&ndash;6 cm&sup2;) handle up to 150 mL and are the largest practical syringe filter format. For volumes above 150 mL, switch to a vacuum bottle-top filter system. Matching filter diameter to sample volume prevents premature membrane clogging and excessive plunger force. Membrane Chemistry for Syringe Filters PES (Polyethersulfone): Low protein binding, high flow rate, aqueous compatibility. Standard choice for biological sample sterilization, cell culture supplement filtration (antibiotics, glutamine, growth factors), and conditioned medium aliquots. Not suitable for strong organic solvents or concentrated acids. PVDF (Polyvinylidene fluoride, hydrophilic): Low protein binding &mdash; among the lowest of all syringe filter membranes, with protein recovery rates &gt;90&ndash;99.9% documented for antibody and enzyme solutions. Compatible with mild organic solvents, weak acids, and most aqueous solutions. The first choice when filtering precious protein solutions (antibodies, enzymes, viral preparations) is where maximum protein recovery is critical. Not suitable for pure DMSO (use PTFE). Nylon (Polyamide): Low extractables, broad chemical compatibility including mixed aqueous-organic solvents, methanol, acetonitrile, and DMSO-water mixtures. Standard for HPLC mobile phase filtration and dissolution testing. Higher protein binding than PES or PVDF &mdash; avoid for biological samples where protein loss is a concern. MCE (Mixed Cellulose Ester): Low cost, aqueous applications only; moderate protein binding. Suitable for general buffer preparation, water sterilization, and non-critical sample preparation. Not compatible with organic solvents or strong acids. CA (Cellulose Acetate): Low protein binding, similar to PES. Suited for biological sample filtration and sterility testing applications. Contains surfactant treatment for wettability &mdash; check for extractable compatibility in downstream assays. PTFE (Polytetrafluoroethylene, hydrophilic): Maximum chemical resistance &mdash; compatible with pure DMSO, concentrated acids and bases, and aggressive organic solvents. The only membrane choice for 100% organic solvent filtration. For aqueous solutions, use hydrophilic PTFE (pre-wetted with methanol or IPA before aqueous filtration unless the hydrophilic version is selected). Sterile vs. Non-Sterile Syringe Filters Sterile syringe filters (individually gamma-irradiated or E-beam sterilized, individually packaged) are required for cell culture media sterilization, supplement preparation, and sample preparation for biological culture. Non-sterile bulk-packed filters (Globe Scientific non-sterile formats) are appropriate for HPLC mobile phase preparation, analytical sample clarification, and non-biological applications where sterility of the filtrate is not required. 
<h2 dir="ltr">Specifications Context</h2>
 Globe Scientific syringe filters are manufactured in a PP housing with a luer-lock inlet and outlet conforming to ISO 594 / ISO 80369 for Luer fittings. PES and PVDF sterile filters are individually gamma irradiated; each is separately wrapped and printed with an expiry date per Globe Scientific lot protocol. Effective filtration areas: 13 mm filter &asymp; 0.92 cm&sup2;; 30 mm filter &asymp; 4.91 cm&sup2; (Globe Scientific welded format). Operating temperature: up to 60 &deg;C. Do not exceed the maximum operating pressure of 6 bar (87 psi). Globe Scientific is ISO 9001:2015 certified. Globe Scientific syringe filters in PES, PVDF, CA, MCE, and nylon membranes at 13 mm and 30 mm diameters are stocked by MBP. 
Request a quote today for premium syringe filters from <a href="https://mbpinc.net/contact-us">the MBP team</a> to&nbsp;find the right filtration solution for your laboratory applications.

DataSheet

Nest Scientific Syringe Filter, PES membrane

380111 by NEST Scientific is a 25 mm, 0.22 um polyethersulfone (PES) syringe filter with USP Grade VI polypropylene housing, individually sterile-wrapped and E-beam sterilized to SAL 10⁻⁶. Low protein binding and high flow rates suit sterile filtration of cell culture media, biological buffers, and antibody solutions. Pyrogen-free, DNase/RNase-free, and cGMP-compliant (21 CFR 820/821). 50/case.

Manufacturer

Syringe Filter, 25mm Housing Diameter, 0.22um PES membrane, Sterile, Individually wrapped, 50/cs

Syringe Filter, 25mm Housing Diameter, 0.45um PES membrane, Sterile, Individually wrapped, 50/cs

Syringe Filter, 13mm Housing Diameter, 0.22um PES membrane, Sterile, Individually wrapped, 50/cs

Syringe Filter, 13mm Housing Diameter, 0.45um PES membrane, Sterile, Individually wrapped, 50/cs

Nest Scientific Syringe Filter, PVDF membrane

380121 by NEST Scientific is a sterile 0.22 µm PVDF syringe filter with a 25 mm housing (4.9 cm² effective area), female Luer lock inlet, male Luer slip outlet, USP Grade VI polypropylene housing, E-beam sterilized to SAL 10⁻⁶, individually wrapped, 50/cs, for HPLC sample preparation, biological solution sterile filtration, and GC sample clarification.

Syringe Filter, 25mm Housing Diameter, 0.22um PVDF membrane, Sterile, Individually wrapped, 50/cs

Syringe Filter, 25mm Housing Diameter, 0.45um PVDF membrane, Sterile, Individually wrapped, 50/cs

Syringe Filter, 13mm Housing Diameter, 0.22um PVDF membrane, Sterile, Individually wrapped, 50/cs

Syringe Filter, 13mm Housing Diameter, 0.45um PVDF membrane, Sterile, Individually wrapped, 50/cs

Erlenmeyer Flasks

<h2 dir="ltr">What are Cell Culture Erlenmeyer Flasks?</h2>
 Cell culture Erlenmeyer flasks (also called shake flasks or conical flasks) are single-use, sterile plastic vessels with a conical body, flat base, and narrow neck used for suspension cell culture on orbital shakers and incubator shakers, microbial culture, and media preparation. Unlike rectangular T-flasks designed for static monolayer culture, Erlenmeyer flasks are engineered for shaking: their wide conical base maximizes liquid surface area for oxygen transfer, while the narrow neck minimizes evaporation and contamination risk. Choose PC Erlenmeyer flasks for autoclavable reuse and high-temperature media preparation; choose PETG for single-use suspension culture and processes requiring BPA-free materials.
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<a href="erlenmeyer-flasks/petg-erlenmeyer-flasks">PETG Erlenmeyer flasks: </a>strong choice for cell culture applications that need excellent clarity, reliable handling, and convenient single-use performance. They are lightweight, durable, and ideal for shaker culture, media preparation, and general suspension cell workflows.
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<a href="erlenmeyer-flasks/pc-erlenmeyer-flasks">PC Erlenmeyer flasks: </a>built for applications that require greater strength and higher impact resistance. They are well-suited for demanding lab environments, including vigorous shaking, transport, and workflows where added durability is important.
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<li dir="ltr" aria-level="1"><a href="https://mbpinc.net/consumables/cell-culture/erlenmeyer-flasks/erlenmeyer-flask-accessories">Erlenmeyer Flask Accessories: </a>Includes bi-directional, inverted, and multifunctional transfer caps, plus silicon tube assemblies for 2 L, 3 L, 3 L wide-mouth, and 5 L high-efficiency flasks, supporting sterile transfer and closed-system workflows.</li>
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<h2 dir="ltr"> How to Choose Erlenmeyer Flasks for Cell Culture</h2>
 PC vs. PETG Material PC (polycarbonate) Erlenmeyer flasks are autoclavable at 121 &deg;C (typically 5&ndash;10 cycles), shatter-proof, crystal-clear, and rated from &minus;135 &deg;C to +135 &deg;C. PC is ideal for labs that sterilize media or wash in-house. PC contains BPA. PETG Erlenmeyer flasks are single-use, BPA-free, rated from &minus;80 &deg;C to +60 &deg;C, and cannot be autoclaved. They provide a contamination-free, cleaning-free alternative to glass and PC for high-throughput suspension culture operations. Flat Base vs. Baffled Base Flat-base Erlenmeyer flasks provide gentler swirling for slow-growth cell lines and media mixing. Baffled-base flasks have three or four angled internal baffles that interrupt the swirling flow, increasing turbulence, raising dissolved oxygen (DO) by up to 2&times; compared to flat-base flasks at the same orbital speed, and reducing cell sedimentation. Baffled flasks are the standard for CHO-S, HEK293-F, Sf9, microbial, and yeast cultures requiring high oxygen transfer rates. NEST Scientific offers baffled and flat-base versions in both PC and PETG across all core volumes. Vented vs. Plug Seal Cap Vented caps contain a 0.22 &micro;m hydrophobic PTFE membrane that allows free O₂/CO₂ gas exchange while excluding microbial contamination. The NEST vented cap features an oversized vent area that improves gas exchange beyond standard-size membrane caps, supporting oxygen-demanding suspension cultures at higher cell densities. Plug seal caps create a fully sealed environment for media storage, static incubation, or transport outside the incubator. Always use vented caps for actively growing suspension cultures on an orbital shaker. Volume and Fill Ratio Erlenmeyer flasks should be filled to no more than 20&ndash;25% of the nominal flask volume for suspension shaker culture to maintain adequate headspace for oxygen transfer. A 500 mL flask running at 20% fill holds 100 mL of culture; a 1,000 mL flask holds 200 mL. Overfilling reduces oxygen transfer efficiency and creates foam that blocks the vented cap membrane. For media storage (no shaking), the fill volume can approach 80% of nominal. Shaker Compatibility NEST Scientific Erlenmeyer flasks (125 mL&ndash;3,000 mL) are compatible with standard laboratory orbital shakers, including those from Infors HT, New Brunswick Scientific (Innova), and Thermo Scientific MaxQ. Confirm flask neck O.D. and base diameter against your shaker's flask holder clips or clamp system before ordering large quantities. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific PC Erlenmeyer flasks are manufactured from USP Class VI medical-grade polycarbonate, gamma sterilized per ISO 11137 to SAL 10⁻⁶, and individually wrapped in sterile packaging. PETG Erlenmeyer flasks are E-beam sterilized and certified DNase/RNase-free, pyrogen-free per lot. Vented cap PTFE membranes are 0.22 &micro;m with an oversized vent area that increases gas exchange efficiency vs. standard-diameter membrane caps. NEST Scientific is ISO 9001:2015 and ISO 13485 certified with FDA registration. Flask catalog sizes extend to high-efficiency 2 L and 5 L PC formats (NEST High Efficiency Erlenmeyer Flask line) for production-scale suspension culture. MBP stocks the NEST Scientific Erlenmeyer flask line across all major volumes and configurations. 
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Support accurate mixing and culturing with premium Erlenmeyer flasks designed for modern laboratories. <a href="https://www.mbpinc.net/contact-us" title="Contact the MBP Team" style="color: #236fa1; text-decoration: underline;">Contact the MBP team</a>&nbsp;for a formal quote.

<h2 dir="ltr">What are PC Erlenmeyer Flasks?</h2>
 PC (polycarbonate) Erlenmeyer flasks are autoclavable, shatter-proof, optically clear shake flasks manufactured from USP Class VI medical-grade polycarbonate for suspension cell culture, microbial fermentation, and media preparation on orbital shakers. Polycarbonate provides a working temperature range of &minus;135 &deg;C to +135 &deg;C and withstands 5&ndash;10 autoclave cycles at 121 &deg;C &mdash; the defining advantage of PC over PETG for labs that steam-sterilize media or require reusable, re-sterilizable shake flasks. PC Erlenmeyer flasks are the correct choice when autoclavability, reuse, or processing at elevated temperature (&gt;60 &deg;C) is required; for single-use BPA-free workflows, PETG Erlenmeyer flasks are the alternative.
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<h2 dir="ltr">What you will find:</h2>
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Vent Filter Caps featuring 0.22&micro;m hydrophobic membranes to facilitate continuous, sterile gas exchange while preventing contamination and evaporation.
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Seal Caps designed for airtight liquid storage, transport, and non-aerated incubation steps.
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Flat Bottom Flasks optimized for uniform heating and stability on standard orbital shakers.
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Baffled Bottom Flasks engineered with integrated "flutes" to increase turbulence and oxygen mass transfer, ideal for high-density yeast and bacterial cultures.
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Volume Scalability with a comprehensive range from 125mL pilots to 5L high-efficiency production vessels, allowing for a seamless transition from bench-scale research to bulk bioprocessing.
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High-Efficiency Wide-Mouth Designs facilitate&nbsp;rapid filling and easy access for large-volume pipetting and automated dispensers.
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<h2 dir="ltr"> How to Choose PC Erlenmeyer Flasks</h2>
 Flat Base vs. Baffled Base for Your Cell Line Flat-base PC flasks produce laminar rotational flow during orbital shaking, providing low-shear mixing appropriate for shear-sensitive primary cells, some hybridoma lines, and media preparation. Baffled-base PC flasks generate turbulence during shaking that substantially increases dissolved oxygen (DO) transfer &mdash; critical for fast-growing CHO-S, HEK293-F, Expi293, bacterial (E. coli, B. subtilis), and yeast cultures where DO depletion limits cell density and productivity. NEST Scientific offers both base geometries across all standard volumes from 125 mL to 3,000 mL, with High Efficiency baffled formats extending to 5,000 mL for production-scale operations. Vented Cap vs. Plug Seal Cap Vented caps (0.22 &micro;m hydrophobic PTFE membrane, HDPE housing) allow continuous gas exchange during shaker culture while maintaining a sterile barrier. The NEST oversized vented cap membrane provides greater gas transfer area than standard-dimension vented caps, supporting high-density cultures. Plug seal caps are gas-impermeable HDPE caps used for static media storage in PC flasks, transport between incubators, and autoclave cycles (caps must be loosened 1&ndash;1.5 turns before autoclaving to prevent pressure buildup). Never use a sealed plug cap on a flask in an orbital shaker incubator &mdash; medium acidification and DO depletion occur within hours. Volume Selection and Fill Ratio NEST Scientific PC flasks are available in 125 mL, 250 mL, 500 mL, 1,000 mL, 2,000 mL, 3,000 mL, and 5,000 mL (High Efficiency). Working fill for shaker culture: 20&ndash;25% of nominal volume. Working volumes: 25&ndash;30 mL in 125 mL; 50&ndash;60 mL in 250 mL; 100&ndash;125 mL in 500 mL; 200&ndash;250 mL in 1,000 mL; 400&ndash;500 mL in 2,000 mL. Scale-up from T-flask to Erlenmeyer: transition from T75 (15&ndash;25 mL static) to a 125 mL PC baffled flask when moving to suspension-adapted cell lines requiring orbital shaking. Autoclave Protocol for PC Flasks PC Erlenmeyer flasks can be autoclaved at 121 &deg;C for 15&ndash;20 minutes (standard gravity cycle). Loosen cap 1&ndash;1.5 turns before autoclaving; retighten after cooling. Repeated autoclaving causes progressive hydrolytic degradation of carbonate linkages &mdash; inspect for crazing, discoloration, or dimensional distortion after each cycle and retire flask at first sign of damage. Do not use autoclaved PC flasks for vacuum applications. BPA Consideration Polycarbonate contains bisphenol A (BPA) as an inherent part of its monomer chemistry. For applications involving estrogen receptor&ndash;positive cell lines (MCF-7, T47D, ZR-75), primary endocrine cells, or long-term culture runs exceeding 14 days, PETG Erlenmeyer flasks (BPA-free) are the safer choice. For standard bacterial, yeast, CHO, and HEK293 suspension culture, trace BPA from USP Class VI PC does not affect results in published culture protocols. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific PC Erlenmeyer flasks are manufactured from USP Class VI medical-grade PC resin in ISO 8 cleanrooms, gamma sterilized per ISO 11137 to SAL 10⁻⁶, and individually wrapped in sterile packaging. PC tensile strength: 55&ndash;75 MPa; maintains impact resistance &gt;50 J/m at &minus;40 &deg;C. Vented cap 0.22 &micro;m hydrophobic PTFE membrane confirmed non-pyrogenic. Flask base diameter and neck geometry are designed for compatibility with standard orbital shaker clips from Infors HT, New Brunswick Innova, and Thermo Scientific MaxQ platforms &mdash; confirm flask O.D. against your specific shaker clamp before bulk ordering. NEST Scientific PC Erlenmeyer flasks up to 5 L (High Efficiency line) are stocked by MBP.
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Connect with the <a href="https://mbpinc.net/contact-us" title="MBP team" style="color: #236fa1; text-decoration: underline;">MBP team</a> today to secure the exact volumes and cap configurations required for your laboratory&rsquo;s next breakthrough.

PC Erlenmeyer Flasks

PC Conical Erlenmeyer Flasks, with Baffles

781201 by NEST Scientific is a 125 mL polycarbonate (PC) Erlenmeyer flask with a baffled bottom, USP Class VI-compliant, sterile, autoclavable to 121 °C, with a polypropylene seal cap. The baffled geometry enhances dissolved oxygen transfer and cell suspension uniformity in orbital shaker culture. Ships 24 per case. Also available in 250 mL, 500 mL, and 1,000 mL.

125 mL PC Conical Erlenmeyer Flasks, with Baffles, Seal Caps, Sterile,1/pk, 24/cs

250 mL PC Conical Erlenmeyer Flasks, with Baffles, Seal Caps, Sterile,1/pk, 12/cs

500 mL PC Conical Erlenmeyer Flasks, with Baffles, Seal Caps, Sterile,1/pk, 12/cs

1000 mL PC Conical Erlenmeyer Flasks, with Baffles, Seal Caps, Sterile,1/pk, 6/cs

2L PC Conical Erlenmeyer Flasks, Flat Base, Seal Cap, Sterile, 1/pk, 6/cs

3L PC Conical Erlenmeyer Flasks, Flat Base, Seal Cap, Sterile, 1/pk, 4/cs

125 mL PC Conical Erlenmeyer Flasks, with Baffles, Vent Filter Caps, Sterile,1/pk, 24/cs

250 mL PC Conical Erlenmeyer Flasks, with Baffles, Vent Filter Caps, Sterile,1/pk, 12/cs

500 mL PC Conical Erlenmeyer Flasks, with Baffles, Vent Filter Caps, Sterile,1/pk, 12/cs

1000 mL PC Conical Erlenmeyer Flasks, with Baffles, Vent Filter Caps, Sterile,1/pk, 6/cs

2L PC Conical Erlenmeyer Flasks, Flat Base, Vent Filter Cap, Sterile, 1/pk, 6/cs

3L PC Conical Erlenmeyer Flasks, Flat Base, Vent Filter Cap, Sterile, 1/pk, 4/cs

Product Literature

PC High Efficient Erlenmeyer Flasks, with Baffles

785105 by NEST Scientific — 2 L USP Class VI polycarbonate high efficiency Erlenmeyer flask with baffled bottom and plug seal cap. E-beam sterile (SAL 10-6), cGMP/ISO 13485 facility, double-bagged. 1/pk, 4/cs.

2L PC High Efficient Erlenmeyer Flasks, with Baffles, Seal Cap, Sterile, 1/pk, 4/cs

2L PC High Efficient Erlenmeyer Flasks, with Baffles, Vent Filter Cap, Sterile, 1/pk, 4/cs

3L PC High Efficient Erlenmeyer Flasks, with Baffles, Seal Cap, Sterile, 1/pk, 4/cs

3L PC High Efficient Erlenmeyer Flasks, with Baffles, Vent Filter Cap, Sterile, 1/pk, 4/cs

5L PC High Efficient Erlenmeyer Flasks, with Baffles, Seal Cap, Sterile, 1/pk, 4/cs

5L PC High Efficient Erlenmeyer Flasks, with Baffles, Vent Filter Cap, Sterile, 1/pk, 4/cs

Media Storage Bottles

<h2 dir="ltr">What are Media Storage Bottles?</h2>
 Media storage bottles for cell culture are sterile, single-use or multi-use plastic containers used to store and dispense tissue culture media, sera, buffers, and other aqueous solutions in research and biopharmaceutical laboratory settings. Choose PC bottles for autoclavable reuse; PET for single-use media storage and transport; PETG for low-temperature archival, closed-system transfer, and pH-sensitive long-term storage.
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<a href="media-storage-bottles/petg-square-bottles">PETG Square Bottles </a>for space-saving laboratory storage
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<a href="consumables/cell-culture/media-storage-bottles/petg-bottle-with-accessories" style="color: #236fa1;">PETG Bottle With</a><a href="media-storage-bottles/petg-bottle-with-accessories" style="color: #236fa1;"> Accessories&nbsp;</a><a href="https://mbpinc.net/petg-bottle-with-accessories" title="PETG Bottle With Accessories" style="color: #236fa1; text-decoration: underline;"></a>for specialized fluid handling
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<a href="media-storage-bottles/pc-media-bottles">PET Media Bottles </a>for high-clarity reagent containment
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<a href="media-storage-bottles/pc-media-bottles">PC Media Bottles </a>for superior mechanical durability
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<h2 dir="ltr"> How to Choose Media Storage Bottles</h2>
 Material: PC vs. PET vs. PETG PC (polycarbonate) bottles are window-clear, shatter-proof, and rated for repeated autoclaving (up to 5&ndash;10 cycles) &mdash; the standard for labs that prepare and sterilize media in-house. PC is resistant to alcohols, weak acids, and aliphatic hydrocarbons; it is not compatible with strong bases or organic solvents. PC contains BPA (bisphenol A) by its monomer chemistry and is not recommended for long-term hormone-sensitive cell culture applications. PET bottles are optically clear, lighter than glass, non-cytotoxic, and non-autoclavable, rated from &minus;60 &deg;C to +60 &deg;C. They are the cost-effective standard for single-use media and sera storage in non-autoclave workflows. PETG (glycol-modified PET) adds a glycol co-monomer that reduces crystallinity, improving impact resistance, low-temperature stability to &minus;80 &deg;C, and flexibility. PETG is the preferred material for long-term storage of pH-sensitive liquids, protein/vaccine intermediates, and applications requiring closed-system accessory assemblies. Round vs. Square Bottles Round bottles (Boston round, GL45 thread) are compatible with standard bottle-top vacuum filtration systems (Millipore Stericup, Corning Costar) and are the format of choice for media vacuum-filtered in-house. Square bottles conserve refrigerator and cold room shelf space due to their flat sides and stackable design, reducing storage footprint by approximately 25% versus equivalent-volume round bottles. NEST Scientific PET and PETG square media bottles feature molded graduations and a white labeling area on two sides. Volume Range Standard media bottle volumes are 60 mL, 125 mL, 250 mL, 500 mL, and 1,000 mL, corresponding to common media and sera kit sizes. A 250 mL bottle accommodates a standard 250 mL media aliquot; a 500 mL bottle holds standard FBS lots or 500 mL media packs. Confirm that the bottle's nominal volume includes 15% additional headspace for mixing, thawing expansion, and transfer operations. Accessory Assembly vs. Standard Cap Standard PETG square media bottles ship with an HDPE screw cap &mdash; suitable for storage and manual transfer. PETG bottles with accessory caps (bi-directional transfer cap + TPE tubing + Luer lock or CPC connector, NEST catalog 3525xx series) create a fully closed aseptic liquid transfer system &mdash; used in cGMP environments, bioprocess research, and any workflow where open-pour media transfer creates contamination or regulatory risk. Sterility and Certifications For cell culture use, all media storage bottles must be certified DNase/RNase-free, non-pyrogenic (endotoxin-free), and non-cytotoxic. NEST Scientific PET and PETG media bottles meet USP Class VI and ISO 10993 biocompatibility requirements, are manufactured in ISO 8 cleanrooms, and are gamma or E-beam-sterilized. PC bottles from autoclave-capable suppliers use virgin Lexan&reg; PC resin compliant with USP Class VI.
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<h2 dir="ltr">Specifications Context</h2>
 NEST Scientific square PET media bottles (catalog 334xxx) and PETG media bottles (catalog 352xxx) are manufactured from 100% virgin material in ISO 8 cleanrooms. Both materials meet USP Class VI and ISO 10993 biocompatibility requirements. Bottles are gamma irradiated in the USA or E-beam sterilized to SAL 10⁻⁶; each lot is certified DNase/RNase-free, pyrogen-free, and endotoxin-free. PETG bottles feature molded graduations (not printed), smooth inner surface to minimize residue on the wall, a one-shot-molded cap seat (no inner gasket required), and a double-bagged sterile packaging format meeting GMP purification workshop material-entry requirements. NEST Scientific PET and PETG media bottles are stocked by MBP in sizes from 60 mL to 1,000 mL. 
Don't wait for a contamination crisis to upgrade your storage. <a href="https://mbpinc.net/contact-us" title="Reach out to the Molecular Biology Products Inc.">Reach out to the Molecular Biology Products Inc.</a> experts for a quote and keep your reagents locked down.

<h2 dir="ltr">What are PET Media Bottles?</h2>
 PET (polyethylene terephthalate) media storage bottles are single-use, sterile, optically clear plastic containers for storing tissue culture media, fetal bovine serum, PBS, HBSS, trypsin-EDTA, and other aqueous laboratory solutions. PET is manufactured by an injection stretch-blow molding (ISBM) process that produces glass-like optical clarity, low gas permeability (especially to CO₂ and O₂ compared to polystyrene), excellent mechanical strength, and a smooth inner surface that minimizes liquid residue. PET bottles are BPA-free, non-autoclavable, and rated for use from &minus;60 &deg;C to +60 &deg;C.
&nbsp;They are the dominant single-use format for media and sera storage in cell culture because of their glass-comparable clarity, low extractable profile, and cost-effectiveness compared to borosilicate glass. Choose PET media bottles as the default single-use format for ready-to-use media and sera storage, cold room archiving, and any application where autoclaving is not required.
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60mL PET Media Bottles for small-scale sampling
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125mL Sterile PET Bottles for routine reagent handling
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250mL PET Storage Bottles for mid-range media volumes
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500mL/0.5L PET Bottles for standard laboratory preparations
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1000mL/1L PET Media Bottles for large-batch stock solutions
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2L High-Volume PET Bottles for industrial-scale storage
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5L PET Media Vessels for bulk liquid containment
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Double-layer Packaged Bottles for enhanced cleanroom safety
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Square Shoulder Tray-Packed Bottles for secure, organized transport
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</ul>
<h2 dir="ltr"> How to Choose PET Media Bottles</h2>
 Round vs. Square Format Round PET media bottles (Boston round, GL45) are directly compatible with bottle-top vacuum filtration systems and standard lab bottle accessories. Square PET media bottles (NEST Scientific catalog 334xxx) feature flat sides that stack efficiently in refrigerators and cold rooms, reducing footprint by approximately 25% versus round bottles and preventing rolling when stored horizontally. NEST PET square bottles have molded graduations (not printed), a white labeling area on two sides, and embossed markings for durable identification. Volume Selection PET media bottles are available in 30 mL, 60 mL, 125 mL, 250 mL, 500 mL, and 1,000 mL volumes. A 500 mL bottle accommodates a standard 500 mL aliquot of cell culture media (DMEM, RPMI, MEM) or a standard FBS lot aliquot. The 1,000 mL bottle holds a full 1 L media preparation. Each bottle includes approximately 15% additional headspace above the nominal volume for thawing expansion (&minus;20 &deg;C frozen FBS thaw) and transfer convenience. Cap Material NEST Scientific PET media bottle caps are made from HDPE (high-density polyethylene), free of heavy metals per regulatory testing. The cap is designed through a one-shot mold process without an inner gasket, fitting securely onto the bottle neck with enhanced sealing performance &mdash; an important feature for leak-proof horizontal storage and air transport. Gas Barrier Properties PET and PETG both reduce gas permeability to CO₂ and O₂ compared to polystyrene, maintaining medium pH and preventing dissolved gas equilibration during storage. For pH-sensitive media containing bicarbonate buffers, PET and PETG are materially superior to polystyrene bottles, which allow CO₂ outgassing and pH shift during storage at room temperature or 4 &deg;C. Sterility and Lot Traceability NEST Scientific PET media bottles are gamma irradiated or E-beam sterilized; each lot is certified DNase/RNase-free, human DNA-free, pyrogen-free, and non-cytotoxic per lot certificate. Lot numbers are printed on each bottle and packaging for traceability. Shelf life is 3 years from the production month per NEST lot release data. PET bottles meet FDA requirements for materials used in contact with sterile liquid pharmaceuticals. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific PET square media bottles are manufactured from 100% virgin PET resin via the ISBM process in ISO 8 cleanrooms. Material meets USP Class VI and ISO 10993 biocompatibility, FDA requirements, and USP &lt;661&gt; container testing for plastics in contact with sterile solutions. Operating temperature: &minus;60 &deg;C to +60 &deg;C; not autoclavable. Smooth inner wall minimizes residue; molded graduations on two sides. The HDPE cap is heavy-metal-free. Packaging is double-bagged in sterile format for GMP-compatible cleanroom entry. Shelf life: 3 years from production month. As of 2026, NEST Scientific PET media bottles are stocked by MBP in the full size range. 
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Don&rsquo;t wait for a compromised sample to upgrade your containment. <a href="https://mbpinc.net/contact-us">Contact now </a>and request a quote from Molecular Biology Products Inc. and keep your lab results (and your PI) stress-free.

PET Media Bottles

PET Square Media Bottles (Sterile)

PET Square Media Bottles, Sterile

Nest Scientific 354611 — 60 mL sterile PET square media bottle, USP Grade VI, E-beam sterilized (SAL 10⁻⁶), DNase/RNase/pyrogen-free, ISO 13485, -70°C to 150°C, 6/pk, 48/case.

60mL PET Square Media Bottles, Sterile, 6/pk, 48/cs

125mL PET Square Media Bottles, Sterile, 6/pk, 48/cs

250mL PET Square Media Bottles, Sterile, 6/pk, 48/cs

500mL PET Square Media Bottles, Sterile, 8/pk, 24/cs

500mL PET Square Media Bottless, Square Shoulder Style, Sterile, 8/pk, 24/cs

1000mL PET Square Media Bottles, Sterile, 4/pk, 12/cs

500 mL PET Square Media Bottles, Square Shoulder Style, Tray-Packed, Sterile, 20/pk, 80/cs

PETG Square Bottles

<h2 dir="ltr">What are PETG Square Media Bottles?</h2>
 PETG square media bottles are single-use, sterile, square-footprint containers manufactured from glycol-modified polyethylene terephthalate (PETG) for storing tissue culture media, fetal bovine serum, PBS, HBSS, buffers, pharmaceutical intermediates, and pH-sensitive aqueous solutions. The square profile reduces cold-room and refrigerator footprint by approximately 25% compared to equivalent-volume round bottles, and flat sides prevent rolling during transport. PETG square bottles are the correct choice over PET when low-temperature stability to &minus;80 &deg;C is needed, when wall durability under cold-room handling is critical, or when closed-system accessory cap assemblies will be used for aseptic liquid transfer.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
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30mL PETG Bottles &ndash; For micro-scale aliquots and stock sampling.
</li>
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60mL PETG Bottles &ndash; Compact size for small-volume buffer storage.
</li>
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125mL PETG Bottles &ndash; Standard size for routine media prep.
</li>
<li dir="ltr" aria-level="1">
250mL PETG Bottles &ndash; Ideal for general cell culture storage.
</li>
<li dir="ltr" aria-level="1">
500mL PETG Bottles &ndash; Optimized for mid-volume biological fluids.
</li>
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1000mL PETG Bottles &ndash; For bulk reagent storage and stock solutions.
</li>
<li dir="ltr" aria-level="1">
2000mL PETG Bottles &ndash; High-capacity for industrial-scale liquid handling.
</li>
</ul>
<h2 dir="ltr"> How to Choose PETG Square Media Bottles</h2>
 PETG vs. PET vs. PC PETG and PET are chemically similar but differ in crystallinity. PETG's glycol modification reduces polymer crystallinity, improving impact resistance, flexibility, and low-temperature stability. PETG is rated from &minus;80 &deg;C to +60 &deg;C; PET from &minus;60 &deg;C to +60 &deg;C; PC from &minus;135 &deg;C to +135 &deg;C. PETG and PET are both BPA-free and non-autoclavable. PC is autoclavable but BPA-containing. For low-temperature archival storage of serum, enzymes, and viral stocks below &minus;60 &deg;C, PETG is the appropriate single-use choice. Volume Selection by Application The 30 mL and 60 mL bottles suit single-use serum aliquots, enzyme dilutions, and small-scale media preparation for T25 or T75 flasks. The 125 mL and 250 mL formats cover standard T75 and T175 media changes. The 500 mL bottle aligns with standard FBS lot sizes and 500 mL media kit volumes. The 1,000 mL bottle stores a full 1 L media preparation or 1 L FBS aliquot. Each bottle includes 15% headspace above nominal volume for expansion during freezing and thawing. Graduated Markings: Molded vs. Printed NEST Scientific PETG square bottles feature molded graduations (not printed), which remain legible through repeated freeze-thaw cycles, solvent exposure, and autoclave label-remover use. Printed graduations on competing formats can abrade or smear over time. Molded graduations are an important quality checkpoint when selecting PETG bottles for long-term archival. Cap Design NEST PETG square bottle caps are HDPE, designed through a one-shot mold process without an inner gasket. The gasket-free design eliminates a potential leachable point and produces a tighter, more consistent seal under the torque applied during manual capping in a cold room or gloved biosafety cabinet environment. The external collar design provides a secure grip for easy sealing under gloves. Double-Bagged GMP Packaging NEST PETG square media bottles are available in double-bagged sterile packaging, meeting material entry requirements for GMP cleanrooms and ISO-classified bioprocess areas. Each inner bag is heat-sealed; the outer bag provides additional sterility protection against outer packaging damage during courier transit. Standard Bottle vs. Accessory Assembly For routine media and serum cold storage, standard PETG square bottles with HDPE screw caps are the appropriate, cost-effective format. When closed-system aseptic liquid transfer between containers is required &mdash; cGMP media dispensing, viral stock aliquoting, pharmaceutical intermediate handling &mdash; choose NEST PETG bottles with bi-directional transfer cap assemblies (TPE tubing + Luer lock or CPC connector). 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific PETG square media bottles are manufactured from 100% virgin PETG resin in ISO 8 (Class 10,000) cleanrooms. Both PET and PETG materials meet USP Class VI and USP &lt;661&gt; biocompatibility requirements and ISO 10993 quality system standards per NEST lot certificates. E-beam sterilization achieves SAL 10⁻⁶. Smooth inner surface minimizes liquid residue on the wall. Operating temperature: &minus;80 &deg;C to +60 &deg;C. HDPE cap is heavy-metal-free per regulatory testing. Shelf life: 3 years from production month. As of 2026, NEST Scientific is ISO 9001:2015 and ISO 13485 certified with FDA registration. 
In a field where a single leak can ruin weeks of data, precision is everything. <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">Contact the Molecular Biology Products Inc. team</a> for a quote and ensure your closed-system transfers stay flawless.

Datsheet

PETG Square Media and Storage Bottles

PETG Square Media Bottles, Sterile

354111 by NEST Scientific is a PETG square media bottle, sterile (E-beam, SAL = 10⁻⁶), USP Class VI, rated −40 °C to 80 °C. Manufactured under cGMP/ISO 13485 in ISO Class 7 cleanrooms; certified DNase-free, RNase-free, and non-pyrogenic for bioprocess and reagent storage.

60mL PETG Square Media Bottles, Sterile, 6/pk, 4/cs

125mL PETG Square Media Bottles, Sterile, 6/pk, 48/cs

250mL PETG Square Media Bottles, Sterile, 6/pk, 48/cs

500mL PETG Square Media Bottles, Sterile, 8/pk, 24/cs

1000mL PETG Square Media Bottless, Sterile, 4/pk, 12/cs

2 L PETG Square Storage Bottle, Sterile, 6/pk, 12/cs

30mL PETG Square Media Bottles, Tray-Packed, Sterile, 40/pk, 280/cs

60mL PETG Square Media Bottles, Tray-Packed, Sterile, 40/pk, 200/cs

125mL PETG Square Media Bottles, Tray-Packed, Sterile, 24/pk, 96/cs

250mL PETG Square Media Bottles, Tray-Packed, Sterile, 30/pk, 60/cs

500mL PETG Square Storage Bottles, Tray-Packed, Sterile, 20/pk, 40/cs

1000mL PETG Square Storage Bottles, Tray-Packed, Sterile, 12/pk, 24/cs

30mL PETG Square Media Bottles, Sterile, 5/pk, 40/cs

BioFactory Accessories

<h2 dir="ltr"> What are Accessories for NEST BioFactory&trade; Systems?</h2>
 NEST BioFactory&trade; accessories are the replacement caps, silicone tubing, vent filters, overcaps, and pre-assembled closed-system cap assemblies used to configure BioFactory units for closed aseptic liquid transfer, gas exchange management, and multi-unit serial connections in cGMP and research-scale bioprocess workflows. The accessory set enables the BioFactory narrow-mouth cap to function as a closed-system liquid transfer port &mdash; media, wash buffer, trypsin, and cell harvest supernatant are transferred in and out through the silicone tubing connection without opening the vessel. Accessories for the BioFactory are required when operating the BioFactory as a closed system; for simple open-pour workflows using wide-mouth caps, standard vent and plug seal caps are the only accessories needed.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="color: #000000;" aria-level="1">
BioFactory media transfer caps
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Sterile tubing and connector sets
</li>
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Fluid transfer adapters for cell culture vessels
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
BioFactory bottle caps and closures
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Sterile quick-connect systems
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Liquid handling accessories
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Vessel handling and support tools
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Closed-system connectors for aseptic processing
</li>
</ul>
<h2 dir="ltr"> Key BioFactory Accessory Categories</h2>
 BioFactory Cap with Silicone Tube (CPC or Luer Connector) The BioFactory cap with silicone tube is the primary closed-transfer accessory: an adaptor cap that fits the narrow-mouth port of the BioFactory unit, pre-assembled with 80 cm of silicone tubing and a terminal connector &mdash; either a male CPC (clamped pressure connector / quick-connect) or a standard Luer lock &mdash; for coupling to peristaltic pumps, other BioFactory units, centrifuge tubes, or PETG media bottle assemblies. Two tubing I.D. &times; O.D. configurations are available: 1/4" I.D. &times; 3/8" O.D. and 3/8" I.D. &times; 5/8" O.D., covering standard peristaltic pump head bore sizes. The female sealing cap included with each assembly caps the connector between uses, maintaining sterility. Overcaps: Vented and Solid Overcaps are secondary dust and contamination barriers placed directly on top of the BioFactory adaptor caps during culture incubation. The vented overcap contains a 0.22 &micro;m hydrophobic breathable membrane, allowing gas exchange through the cap stack while blocking particles and microbial contamination &mdash; used during active shaker or orbital incubation when the unit's primary cap is an adaptor cap rather than a vented cap. The solid overcap provides an additional dust and contamination seal with no gas exchange &mdash; used for short-term storage or transport outside the incubator. SPT-50 Silicone Hose SPT-50 silicone hose (3/8" I.D. &times; 5/8" O.D., 50 inches / 15 meters) is the bulk tubing used to connect multiple BioFactory units in series, connect BioFactory ports to peristaltic pump heads, or extend the reach of the transfer cap assembly. SPT-50 tubing is manufactured from silicone, is individually wrapped and sterile, and passes dissolution testing per BPOG (BioPhorum Operations Group) criteria per NEST product documentation, supporting its use in biopharmaceutical production environments. Premium Accessory Package NEST offers a BioFactory Accessory Premium Package that includes 2 different adaptor caps, 1 hose clamp, 1 adaptor connector, 1 vent filter (50 mm), 1 additional hose clamp, and 15 m of SPT-50 silicone hose &mdash; all individually wrapped and sterile in a single kit. This package provides all components needed to set up a closed-system BioFactory media management workflow from a single kit, reducing the number of individual part orders required. Vent Filters for Gas Exchange Management For BioFactory units using adaptor caps rather than standard vented caps, 50 mm inline vent filters (0.22 &micro;m hydrophobic PTFE membrane) are installed on the air inlet/outlet tubing line to maintain sterile gas exchange during media addition, harvest, and incubation. The vent filter is rated for high flow rates without creating excessive back-pressure that would resist liquid movement through the system. Compatibility: Narrow-Mouth BioFactory Units Only All BioFactory accessory caps and tubing assemblies are designed exclusively for the narrow-mouth BioFactory configuration. Wide-mouth BioFactory units use standard wide-mouth vent and plug seal caps only; they are not compatible with accessory cap assemblies. Confirm that your BioFactory units have narrow-mouth ports before ordering the accessory cap set. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST BioFactory&trade; accessory silicone tubing and cap assemblies are manufactured from USP Class VI silicone (TPE/silicone) and polycarbonate or PP cap bodies, individually wrapped and sterile. All components have passed dissolution testing per BPOG dissolution test criteria conducted by a certified third-party laboratory per NEST Scientific documentation. The standard Luer head connections conform to ISO 594 / GB/T 1962 for reliable compatibility with standard laboratory fittings. Vent filters use a 0.22 &micro;m hydrophobic PTFE membrane; overcaps use a 0.22 &micro;m hydrophobic membrane. NEST BioFactory accessories are carried by MBP alongside BioFactory units. 
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Need the right BioFactory setup? <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact MBP today</a> for technical assistance.

Biofactory System Accessories

SPT-50 Hose，Inner dia# 3/8 Inches(9.5 mm)，Out dia# 5/8 Inches(15.9 mm), 50 Inches/ 15 meters, Individually Wrapped, Sterile, 1/pk, 1/cs

744001 is the SPT-50 platinum-cured silicone hose: inner diameter 3/8 inch (9.5 mm), outer diameter 5/8 inch (15.9 mm), 15 meters length. 50 Shore A durometer, USP Class VI compliant, European Pharmacopoeia 3.1.9 certified. Sterile, individually wrapped. Suitable for peristaltic pump fluid transfer, sterile-fill manufacturing, and GMP bioprocess lines. 1/pk, 1/cs.

Erlenmeyer Flask Accessories

Seal Caps & Vent Caps for Erlenmeyer Flasks

781925 by NEST Scientific includes both seal caps and vent caps for Erlenmeyer flasks used in suspended cell culture, medium preparation, and storage. Vent caps feature a 0.22 µm hydrophobic PTFE membrane for sterile gas exchange during orbital shaker culture. Both types are USP Grade VI polypropylene, E-beam sterilized to SAL 10⁻⁶, cGMP 21 CFR Part 820 compliant.

Seal Cap for 125mL Erlenmeyer Flasks, 1/pk, 25/cs

Vent Cap for 125mL Erlenmeyer Flasks, 1/pk, 25/cs

Seal Cap for 250mL Erlenmeyer Flasks, 1/pk, 25/cs

Vent Cap for 250mL Erlenmeyer Flasks, 1/pk, 25/cs

Seal Cap for 500&1000mL Erlenmeyer Flasks, 1/pk, 25/cs

Vent Cap for 500&1000 mL Erlenmeyer Flasks, 1/pk, 25/cs

Seal Cap for 2L Erlenmeyer Flasks, 1/pk, 20/cs

Vent Cap for 2L Erlenmeyer Flasks, 1/pk, 20/cs

Seal Cap for 3L Erlenmeyer Flasks/ 2&3 L High Efficient Erlenmeyer Flasks, 1/pk, 20/cs

Vent Cap for 3L Erlenmeyer Flasks/ 2&3 L High Efficient Erlenmeyer Flasks, 1/pk, 20/cs

Seal Cap for 3L Wide-mouth/5L Efficient Erlenmeyer Flasks, 1/pk, 20/cs

Vent Cap for 3L Wide-mouth/5L Efficient Erlenmeyer Flasks, 1/pk, 20/cs

T Shape CPC Connector for Inner Dia #3/8 Hose, Individually Wrapped, Sterile, 1/pk, 5/cs

NEST Scientific 749001: sterile T-shape CPC connector for 3/8" ID hose in single-use bioprocess systems. Lateral 90° port for sampling, bypass, or branching while main flow continues. Individually wrapped, 1/pk, 5/cs. Order at <a href="https://www.mbpinc.net/quickorder">mbpinc.net/quickorder</a>.

Cell Culture Flasks

<h2 dir="ltr">What are Cell Culture Flasks?</h2>
 Cell culture flasks (T-flasks) are sterile, rectangular polystyrene vessels with a canted, angled neck designed to house adherent or suspension cell cultures in a CO₂ incubator. They are available in growth surface areas from T25 (25 cm&sup2;) to T225 (225 cm&sup2;), with either tissue culture&ndash;treated (TC-treated) surfaces for adherent cells or non-treated surfaces for suspension and microbial culture. Cap options include vented caps with 0.2 &micro;m hydrophobic membranes for CO₂ gas exchange, and plug seal caps for anaerobic or sealed transport.&nbsp; Choose the flask size based on the number of cells needed for downstream applications: T25 for seed stocks, T75 for routine maintenance, T175 and T225 for scale-up and protein production runs.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
<a href="cell-culture-flasks/treated-cell-culture-flasks" title="Treated Cell Culture Flasks" style="color: #236fa1;">Treated Cell Culture Flasks</a>: Vacuum-plasma modified surfaces that enhance hydrophilic interaction for uniform monolayer formation.
</li>
<li dir="ltr" aria-level="1">
<a href="cell-culture-flasks/non-treated-cell-culture-flasks" title="Non-Treated Cell Culture Flasks:">Non-Treated Cell Culture Flasks:</a> Naturally hydrophobic polystyrene designed specifically for hybridomas and non-adherent suspension cultures.
</li>
</ul>
<h2 dir="ltr"> How to Choose Cell Culture Flasks</h2>
 Size by Growth Area and Working Volume T25 flasks (25 cm&sup2;, working volume 5&ndash;10 mL) are used for seed stocks, small-scale experiments, and initial passages of new cell lines where preservation of limited cells is critical. T75 flasks (75 cm&sup2;, 15&ndash;25 mL) are the routine maintenance format for most adherent cell lines in research settings. T175 flasks (175 cm&sup2;, 35&ndash;50 mL) suit scale-up for protein production, viral vector manufacturing, and high-yield transient transfection. T225 flasks (225 cm&sup2;, 50&ndash;70 mL) provide maximum single-flask growth area before scale-up to multi-layer vessels. TC-Treated vs. Non-Treated Surface TC-treated flasks (vacuum plasma treated, contact angle &lt;10&deg;) are essential for adherent mammalian cell lines &mdash; HeLa, HEK293, CHO (adherent), Vero, A549, NIH 3T3, and primary cells, including fibroblasts, epithelial cells, and endothelial cells. Non-treated flasks maintain the original hydrophobic PS surface, preventing adherent growth &mdash; used for suspension CHO, Jurkat, K562, hematopoietic cells, hybridomas, Sf9/Hi5 insect cells, and bacterial culture. Canted vs. Straight Neck A canted (angled) neck on the flask allows the flask to rest at an angle when standing, keeping the growth surface slightly tilted during feeding and pipetting. This angled geometry improves access for serological pipettes and reduces the risk of contaminating the culture surface during media changes. All NEST Scientific T-flasks use a canted neck design. Cap Type and Gas Exchange Vented caps with 0.2 &micro;m hydrophobic membranes are the standard for CO₂ incubator use. The 0.2 &micro;m pore size blocks microbial contamination while allowing CO₂ and O₂ to equilibrate freely, maintaining medium pH through the bicarbonate buffering system. Plug seal caps isolate the flask from the ambient atmosphere &mdash; used for short-term transport, sealed storage, and anaerobic culture applications. Some protocols require alternating between vent and plug seal during different culture phases. Sterility and Traceability All NEST Scientific cell culture flasks carry E-beam sterilization (ISO 11137 equivalent SAL 10⁻⁶), DNase/RNase-free and pyrogen-free lot certification, batch traceability codes, and are produced under ISO 9001:2015 and ISO 13485 quality management. Flasks are individually packaged in peelable Tyvek sleeves or resealable sterile bags. Each flask features two large labeling areas for cell line identification and passage number notation.
<h2 dir="ltr">&nbsp;</h2>
<h2 dir="ltr">Compare Cell Culture Flask Sizes </h2>
&nbsp;
<ul>
<li>T25: Growth Area: 25 cm&sup2;; Working Volume: 5&ndash;10 mL; Typical Cell Yield (adherent): 5&ndash;8 &times; 10⁶; Best Application: Seed stock, new cell line setup.</li>
<li>T75: Growth Area: 75 cm&sup2;; Working Volume: 15&ndash;25 mL; Typical Cell Yield (adherent): 15&ndash;25 &times; 10⁶; Best Application: Routine passage and maintenance.</li>
<li>T175: Growth Area: 175 cm&sup2;; Working Volume: 35&ndash;50 mL; Typical Cell Yield (adherent): 35&ndash;60 &times; 10⁶; Best Application: Scale-up, transient transfection.</li>
<li>T225: Growth Area: 225 cm&sup2;; Working Volume: 50&ndash;70 mL; Typical Cell Yield (adherent): 50&ndash;80 &times; 10⁶; Best Application: High-yield expansion, virus production.</li>
</ul>
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific cell culture flasks (T25 catalog 707001, T75 catalog 708001, T175 catalog 709001, T225 catalog 710001) are manufactured from 100% virgin polystyrene in an ISO 8 (Class 10,000) cleanroom. Vacuum plasma TC treatment creates a surface contact angle of &lt;10&deg; (hydrophilic) per NEST lot data, verified by water contact angle goniometry. Vented caps contain a hydrophobic 0.2 &micro;m PTFE membrane that passes the USP Particulate Standard for injectable preparations &mdash; confirmed free of fibers and particulates at lot release. Flask footprints are standard for incubator shelf spacing: T25 width 100 mm, T75 width 150 mm, T175/T225 width 167 mm. As of 2026, NEST Scientific flasks are stocked by MBP for same-case reorder by PO. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center. 
&nbsp;
Eliminate variables in the incubator to ensure absolute integrity in your results with MBP's Cell Culture Flasks! <a href="https://mbpinc.net/contact-us">Reach out to our team</a> today for volume-based pricing.

<h2 dir="ltr">What are TC-Treated Cell Culture Flasks?</h2>
 Tissue culture&ndash;treated (TC-treated) cell culture flasks are sterile polystyrene T-flasks whose inner growth surface has been modified by vacuum plasma treatment to introduce negatively charged, oxygen-containing functional groups (carboxyl, carbonyl, hydroxyl) that reduce the water contact angle from ~75&ndash;90&deg; (hydrophobic, untreated PS) to below 10&deg; (hydrophilic). This surface modification directly promotes cell attachment, spreading, and cytoskeletal organization in adherent mammalian cell lines. Without TC treatment, virtually all primary mammalian cells and established adherent lines, including HeLa, HEK293 (adherent), CHO-K1, Vero, A549, NIH 3T3, MDCK, and primary fibroblasts, fail to attach and undergo anoikis within 24&ndash;48 hours. TC-treated flasks are the correct default for any adherent mammalian cell line unless the experimental protocol explicitly specifies non-treated or specialized surfaces (ultra-low attachment, poly-D-lysine, collagen I).
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
TC-Treated Surfaces: Vacuum-plasma modified polystyrene for maximum cellular adhesion and uniform growth kinetics.
</li>
<li dir="ltr" aria-level="1">
Gas Exchange Efficiency: Vented caps with 0.22&micro;m hydrophobic membranes ensure sterile CO2 exchange while preventing contamination.
</li>
<li dir="ltr" aria-level="1">
Scalable Geometry: Available in T25, T75, T175, and T225 formats to support both routine maintenance and high-yield production.
</li>
<li dir="ltr" aria-level="1">
Advanced Ergonomics: The angled neck design facilitates complete access for cell scrapers and serological pipettes, minimizing media waste.
</li>
<li dir="ltr" aria-level="1">
Optical Excellence: USP Class VI high-clarity material provides distortion-free microscopy for precise monitoring.
</li>
<li dir="ltr" aria-level="1">
Certified Purity: Validated RNase, DNase, and Pyrogen-free to ensure experimental data integrity.
</li>
</ul>
<h2 dir="ltr"> How to Choose TC-Treated Cell Culture Flasks</h2>
 Size by Growth Area and Workflow Stage T25 (25 cm&sup2;, 5&ndash;10 mL): seed stocks, low-passage critical cell lines, newly thawed cells, and experiments requiring fewer than ~8 &times; 10⁶ cells. T75 (75 cm&sup2;, 15&ndash;25 mL): the standard research-lab flask for routine weekly passage of most adherent cell lines; the most commonly stocked format. T175 (175 cm&sup2;, 35&ndash;50 mL): scale-up for transient transfection (lipofection, calcium phosphate, PEI), viral vector production (lentivirus, AAV), and protein expression experiments requiring 35&ndash;60 &times; 10⁶ adherent cells per flask. T225 (225 cm&sup2;, 50&ndash;70 mL): maximum single-flask density before transitioning to multi-layer BioFactory vessels; used for large-scale CHO adherent culture and high-yield antibody or virus production runs. Vented vs. Plug Seal Cap Vented caps with a 0.2 &micro;m hydrophobic PTFE membrane allow free CO₂/O₂ gas exchange &mdash; mandatory for cells buffered by the bicarbonate/CO₂ system in a CO₂ incubator (typically 5&ndash;10% CO₂). Plug seal caps create a sealed environment; use them for short-term transport between incubators, for HEPES-buffered media that do not require CO₂, or for anaerobic culture. Using a plug seal cap in a standard CO₂ incubator blocks gas exchange and acidifies the medium within 2&ndash;4 hours. Straight vs. Canted Neck Straight-neck flasks are the traditional geometry; canted (angled) neck flasks tilt the growth surface away from the neck, improving pipette access during media changes and cell harvesting. NEST Scientific uses a canted neck design across all flask sizes. Confirm your biosafety cabinet arm reach and pipette length before committing to one geometry in bulk. Lot-to-Lot Consistency For long-running experiments, drug screening, or validated bioprocess workflows, lot-to-lot TC treatment consistency is critical. NEST Scientific verifies contact angle on each manufacturing lot. Switching the flask lots mid-experiment can alter cell attachment density, proliferation rate, and morphology. Order sufficient flasks from a single lot for the duration of a defined experimental series. Cells That Require TC-Treated Surfaces HeLa, A549, Vero (BSC-1), HEK293 (adherent), CHO-K1 (monolayer), MDCK, NIH 3T3, MCF-7, U2OS, PC-3, HepG2, primary dermal fibroblasts, primary endothelial cells, primary epithelial cells, and most primary culture preparations require TC-treated surfaces. Cells that must NOT be cultured on TC-treated surfaces include Jurkat, K562, HL-60, suspension CHO-S, hybridomas, Sf9/Hi5 insect cells, and hematopoietic progenitor cells &mdash; use non-treated flasks for all of these. 
<h2 dir="ltr"> Specifications Context </h2>
 NEST Scientific TC-treated flasks are manufactured from 100% virgin polystyrene in ISO 8 (Class 10,000) cleanrooms. Vacuum plasma TC treatment is verified by water contact angle goniometry per manufacturing lot; contact angle &lt;10&deg; is the release criterion. E-beam sterilization achieves SAL 10⁻⁶; flasks are DNase/RNase-free and non-pyrogenic per lot certificate. Vented cap 0.2 &micro;m PTFE hydrophobic membrane is certified free of particles per USP Particulate Standard. Flask footprint dimensions comply with standard CO₂ incubator shelf spacing: T25 (100 mm width), T75 (150 mm), T175/T225 (167 mm). Double-layered Tyvek packaging maintains sterility until the point of use. NEST Scientific holds ISO 9001:2015, ISO 13485, and ISO 11137 certifications, and has FDA registration and CE marking. As of 2026, MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center.
 <a href="https://mbpinc.net/contact-us">Connect with the helpful team at MBP </a>today to secure a customized quote for your facility.

Treated Cell Culture Flasks

<h2 dir="ltr">What are Non-Treated Cell Culture Flasks?</h2>
 Non-treated cell culture flasks are sterile polystyrene T-flasks that retain the original hydrophobic, unmodified surface of the polystyrene substrate &mdash; no plasma, corona, or chemical surface treatment is applied. The hydrophobic surface minimizes cell attachment, making these flasks the correct vessel for cell types that grow in suspension or require minimal surface contact: suspension-adapted CHO, Jurkat, K562, HL-60, hematopoietic progenitors, hybridomas for monoclonal antibody production, insect cell lines (Sf9, Hi5), yeast, and bacteria. NEST Scientific non-treated flasks are manufactured from 100% virgin polystyrene in ISO 8 cleanrooms, E-beam sterilized to SAL 10⁻⁶, and available in T25, T75, T175, and T225 sizes with vented (0.2 &micro;m PTFE membrane) or plug seal caps. 
Use non-treated flasks exclusively for cell types that do not require surface attachment; placing adherent mammalian cell lines in a non-treated flask causes cell death within 24&ndash;48 hours. 
<h2 dir="ltr">What you will find:</h2>
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Suspension-Optimized Surface, like naturally hydrophobic polystyrene, ensures cells remain in suspension without surface interference.
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Scalable Formats precision-engineered in T25, T75, T175, and T225 sizes to fit various research scales.
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Aseptic Security with certified RNase-, DNase-, and Pyrogen-free to maintain the highest purity standards.
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Ergonomic Access with angled neck design facilitates seamless pipette access and reduces the risk of contamination during media changes.
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<h2 dir="ltr">How to Choose Non-Treated Cell Culture Flasks</h2>
 Application: Suspension Mammalian Cells Suspension-adapted cell lines &mdash; CHO-S, HEK293-F, Expi293, and HEK293-EBNA variants used in transient transfection &mdash; require non-treated flasks for static suspension culture between spinner flask passages or when the scale is too small for orbital shaker flasks. Cells must be agitated (shaken, pipetted, or rocked) periodically to prevent settling when in static non-treated flasks with volumes &gt;5 mL. Application: Hematopoietic and Immune Cells Non-adherent hematopoietic cell lines (Jurkat T-cells, K562 erythroleukemia, HL-60 promyelocytes, Raji B-cells) and primary hematopoietic cells (peripheral blood mononuclear cells (PBMCs), bone marrow progenitors) must be cultured in non-treated vessels. TC-treated surfaces activate integrin signaling in hematopoietic cells, inducing undesired differentiation and stress responses. Application: Hybridoma Culture Hybridoma cells for monoclonal antibody (mAb) production are non-adherent B-cell fusions that grow as suspension cultures. Non-treated T75 and T225 flasks, combined with HT-supplemented DMEM/HAT medium, are the standard vessels for hybridoma selection and expansion. Scale-up from T225 non-treated flasks typically progresses to spinner or hollow-fiber bioreactors. Application: Insect Cells Sf9 (Spodoptera frugiperda) and Hi5 (Trichoplusia ni) insect cells used in baculovirus expression vector systems (BEVS) require non-treated polystyrene for monolayer culture. On TC-treated surfaces, Sf9 cells attach so strongly that they are difficult to recover. Non-treated flasks allow Sf9 to loosely adhere and detach easily with tapping, or to grow as a loosely attached monolayer that tolerates trypsin-free passaging. Application: Microbial Culture Non-treated polystyrene T-flasks with plug seal caps can serve as culture vessels for E. coli, Bacillus subtilis, yeast (S. cerevisiae, P. pastoris), and other microorganisms where the large surface area per volume is needed, and standard Erlenmeyer flask orbital shaking is unavailable. Ventilated caps support aerobic culture; confirm that the vented 0.2 &micro;m membrane is compatible with your aeration requirements. Cap Selection for Non-Treated Flasks Vented caps (0.2 &micro;m PTFE membrane) are standard for suspension cultures in a CO₂ incubator. For microbial aerobic culture without a CO₂ incubator, vented caps with 0.2 &micro;m pore still provide adequate aeration. Plug seal caps are used for anaerobic culture, transport, and short-term storage.
<h2> Specifications Context</h2>
 NEST Scientific non-treated cell culture flasks are manufactured from 100% virgin polystyrene with no surface modification. Contact angle of non-treated PS surface is approximately 75&ndash;90&deg; (hydrophobic), confirmed by water contact angle measurement per NEST lot QC data. E-beam sterilized in ISO 8 cleanrooms to SAL 10⁻⁶; DNase-free, RNase-free, and non-pyrogenic per lot certificate. Vented caps use a 0.2 &micro;m hydrophobic PTFE membrane. As of 2026, NEST Scientific non-treated flasks are stocked by MBP in all four sizes (T25, T75, T175, T225) with vented and plug seal options, in case quantities for PO-based institutional ordering. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center. 
Elevate your suspension culture workflows. <a href="https://mbpinc.net/contact-us">Contact Molecular Biology Products Inc.</a> for your Non-Treated Cell Culture Flasks today!

Non-Treated Cell Culture Flasks

T150 U-Shaped Canted Neck Cell Culture Flask Specifications

T150 U-Shaped Canted Neck Cell Culture Flask

NEST Scientific 720001 is a T150 U-shaped canted-neck cell culture flask with 150 cm² TC-treated growth surface, USP Class VI medical-grade polystyrene, plug seal cap, frosted marking area, and volume graduations. Sterile, 5/pk, 40/cs. For adherent mammalian cell expansion and subculture.

150cm2 U-Shaped Canted Neck Cell Culture Flask, Plug Seal Cap, TC, sterile 5/pk, 40/cs

150cm2 U-Shaped Canted Neck Cell Culture Flask, Plug Seal Cap, Non-Treated, sterile 5/pk, 40/cs

150cm2 U-Shaped Canted Neck Cell Culture Flask, Vent Cap, Non-Treated, sterile 5/pk, 40/cs

150cm2 U-Shaped Canted Neck Cell Culture Flask, Vent Cap, TC, sterile 5/pk, 40/cs

Vacuum Filtration

<h2 dir="ltr">What are Vacuum Bottle-Top Filter Systems?</h2>
 Vacuum bottle-top filter systems are single-use, sterile filtration assemblies consisting of a graduated polystyrene funnel with an integrated membrane filter bonded to a polystyrene receiver bottle, connected by a hose-port adaptor to a house vacuum line or benchtop vacuum pump. The applied negative pressure (&minus;50 to &minus;100 kPa) draws liquid through the membrane into the receiver bottle, which is sealed after filtration for immediate use or cold-room storage.&nbsp;
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<h2 dir="ltr">What you will find:</h2>
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50mL Vacuum Filtration Units for small-scale sterile sampling
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150mL Vacuum Filtration Systems for rapid reagent preparation
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250mL Filtration Units for routine buffer sterilization
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500mL Vacuum Filtration Cups for mid-scale media processing
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1000mL Large Volume Filtration for industrial-scale batching
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Sterile Bottle-Top Filters for secure aseptic transfer
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<h2 dir="ltr"> How to Choose Vacuum Filtration Systems</h2>
 PES vs. PVDF Membrane for Vacuum Filtration PES membranes provide low protein binding (~1&ndash;2 &micro;g/cm&sup2;), high flow rates, and broad aqueous-solution compatibility &mdash; the standard for routine DMEM, RPMI, HBSS, PBS, and supplement-containing media sterilization. PVDF membranes have slightly lower protein binding than PES and greater chemical resistance to mild organic solvents, mild acids, and alkaline solutions. Use PVDF when filtering protein-rich solutions (FBS, antibody preparations, conditioned medium with high protein content) or when mild organic solvents are present in the filter solution. Volume Selection 250 mL systems (50 mm diameter membrane, 50 mL funnel) suit small media aliquots, supplement solutions, and transfection reagent preparations. 500 mL systems (90 mm diameter membrane, 150 mL funnel) are the routine lab workhorse for standard 500 mL media bottle preparation and FBS filtration. 1,000 mL systems (90 mm diameter membrane, 350 mL funnel) handle full-liter media preparations and large-volume buffer sterilization. All NEST systems include a pre-attached, graduated polystyrene receiver bottle in the corresponding volume. Pore Size: 0.10 &micro;m vs. 0.22 &micro;m vs. 0.45 &micro;m 0.22 &micro;m is the sterilizing grade per USP &lt;1229.2&gt; and ICH Q9: retains all bacteria and fungi; used for routine cell culture media, PBS, and buffer preparation. 0.45 &micro;m is the clarification grade: used as a pre-filtration step before 0.22 &micro;m to remove large particulates from turbid or high-biomass samples (FBS, conditioned media, cell culture supernatants, crude lysates), extending 0.22 &micro;m filter throughput by 3&ndash;5&times; on difficult samples. 0.10 &micro;m is the mycoplasma-removal grade: required when preparing media for Mycoplasma-sensitive primary cell culture or when processing serum or supplementary reagents that may harbor Mycoplasma species. Note that 0.10 &micro;m filtration is substantially slower than 0.22 &micro;m due to the tighter membrane pore structure. Practical Tips for Maximum Throughput Pre-wet the membrane by briefly passing 5&ndash;10 mL of sterile water through before the sample &mdash; this wets the hydrophilic PES or PVDF membrane and reduces initial resistance. For FBS and other viscous solutions, pre-filter through 0.45 &micro;m before the 0.22 &micro;m step. Maintain a vacuum at &minus;50 to &minus;80 kPa; excessive vacuum (&gt; &minus;100 kPa) can shear fragile membrane pores and increase non-specific protein adsorption. Do not autoclave the assembled bottle-top filter &mdash; PES and PS components are not rated for autoclave sterilization. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific vacuum bottle-top filters are manufactured from polystyrene funnel and receiver bottle components with PES or PVDF membranes. PES membranes have documented low protein binding (~1&ndash;2 &micro;g/cm&sup2;) per NEST scientific USA FAQ. Membrane diameter: 250 mL volume &rarr; 50 mm; 500 mL and 1,000 mL &rarr; 90 mm. The hose connector design accommodates various standard vacuum hose diameters. NEST PES 0.22 &micro;m vacuum filters are described as comparable in performance to leading brands, including Corning Stericup, Thermo Fisher Scientific, and Sartorius, per NEST Scientific USA's product page. All NEST vacuum filters are sterile and non-pyrogenic per the lot certificate. NEST Scientific vacuum bottle-top filters are stocked by MBP in all core SKUs. 
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Because your research should move faster than a slow-dripping filter. <a href="https://mbpinc.net/contact-us" title="Contact the Molecular Biology Products Inc. team" style="color: #236fa1;">Contact the Molecular Biology Products Inc. team</a> for a quote before your deadlines catch up with you.

Vacuum Filtration Systems  (PES Membrane)

Vacuum Filtration Systems, PES, Sterile

347002 by NEST is a sterile, disposable vacuum filtration system with a PES membrane. Available in five volumes (50–1000 mL) and three pore sizes (0.10 µm, 0.22 µm, 0.45 µm) as selectable variants. Polystyrene USP Grade IV cup with ABS neck, E-beam sterilized to SAL 10⁻⁶. Non-pyrogenic, DNase/RNase-free, animal product-free. 12/case.

50 mL Vacuum Filtration, 0.10 μm, PES, Sterile, 1/pk, 12/cs

50 mL Vacuum Filtration, 0.22 μm, PES, Sterile,1/pk, 12/cs

50 mL Vacuum Filtration, 0.45 μm, PES, Sterile, 1/pk, 12/cs

150 mL Vacuum Filtration, 0.10 μm, PES, Sterile, 1/pk, 12/cs

150 mL Vacuum Filtration, 0.22 μm, PES, Sterile,1/pk, 12/cs

150 mL Vacuum Filtration, 0.45 μm, PES, Sterile, 1/pk, 12/cs

250 mL Vacuum Filtration, 0.10 μm, PES, Sterile, 1/pk, 12/cs

250 mL Vacuum Filtration, 0.22 μm, PES, Sterile, 1/pk, 12/cs

250 mL Vacuum Filtration, 0.45 μm, PES, Sterile, 1/pk, 12/cs

500 mL Vacuum Filtration, 0.10 μm, PES, Sterile, 1/pk, 12/cs

500 mL Vacuum Filtration, 0.22 μm, PES, Sterile, 1/pk, 12/cs

500 mL Vacuum Filtration, 0.45 μm, PES, Sterile, 1/pk, 12/cs

1000 mL Vacuum Filtration, 0.10 μm, PES, Sterile, 1/pk, 12/cs

1000 mL Vacuum Filtration, 0.22 μm, PES, Sterile, 1/pk, 12/cs

1000 mL Vacuum Filtration, 0.45 μm, PES, Sterile, 1/pk, 12/cs

Vacuum Filtration Systems (PVDF Membrane)

Vacuum Filtration Systems, PVDF, Sterile

347102 by NEST Scientific is a sterile disposable vacuum filtration system with a PVDF membrane, E-beam sterilized (SAL 10⁻⁶), DNase/RNase-free, and endotoxin-free. The PVDF membrane offers low protein binding and high flow rates for cell culture media sterilization, buffer filtration, and protein solution processing. PS filter top and PS receiving bottle with ABS neck; separate sterile PE cap included. Multiple volume options available.