Cell Culture Flasks

<h2 dir="ltr">What are Cell Culture Flasks?</h2>
 Cell culture flasks (T-flasks) are sterile, rectangular polystyrene vessels with a canted, angled neck designed to house adherent or suspension cell cultures in a CO₂ incubator. They are available in growth surface areas from T25 (25 cm&sup2;) to T225 (225 cm&sup2;), with either tissue culture&ndash;treated (TC-treated) surfaces for adherent cells or non-treated surfaces for suspension and microbial culture. Cap options include vented caps with 0.2 &micro;m hydrophobic membranes for CO₂ gas exchange, and plug seal caps for anaerobic or sealed transport.&nbsp; Choose the flask size based on the number of cells needed for downstream applications: T25 for seed stocks, T75 for routine maintenance, T175 and T225 for scale-up and protein production runs.
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<h2 dir="ltr">What you will find:</h2>
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<a href="cell-culture-flasks/treated-cell-culture-flasks" title="Treated Cell Culture Flasks" style="color: #236fa1;">Treated Cell Culture Flasks</a>: Vacuum-plasma modified surfaces that enhance hydrophilic interaction for uniform monolayer formation.
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<a href="cell-culture-flasks/non-treated-cell-culture-flasks" title="Non-Treated Cell Culture Flasks:">Non-Treated Cell Culture Flasks:</a> Naturally hydrophobic polystyrene designed specifically for hybridomas and non-adherent suspension cultures.
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<h2 dir="ltr"> How to Choose Cell Culture Flasks</h2>
 Size by Growth Area and Working Volume T25 flasks (25 cm&sup2;, working volume 5&ndash;10 mL) are used for seed stocks, small-scale experiments, and initial passages of new cell lines where preservation of limited cells is critical. T75 flasks (75 cm&sup2;, 15&ndash;25 mL) are the routine maintenance format for most adherent cell lines in research settings. T175 flasks (175 cm&sup2;, 35&ndash;50 mL) suit scale-up for protein production, viral vector manufacturing, and high-yield transient transfection. T225 flasks (225 cm&sup2;, 50&ndash;70 mL) provide maximum single-flask growth area before scale-up to multi-layer vessels. TC-Treated vs. Non-Treated Surface TC-treated flasks (vacuum plasma treated, contact angle &lt;10&deg;) are essential for adherent mammalian cell lines &mdash; HeLa, HEK293, CHO (adherent), Vero, A549, NIH 3T3, and primary cells, including fibroblasts, epithelial cells, and endothelial cells. Non-treated flasks maintain the original hydrophobic PS surface, preventing adherent growth &mdash; used for suspension CHO, Jurkat, K562, hematopoietic cells, hybridomas, Sf9/Hi5 insect cells, and bacterial culture. Canted vs. Straight Neck A canted (angled) neck on the flask allows the flask to rest at an angle when standing, keeping the growth surface slightly tilted during feeding and pipetting. This angled geometry improves access for serological pipettes and reduces the risk of contaminating the culture surface during media changes. All NEST Scientific T-flasks use a canted neck design. Cap Type and Gas Exchange Vented caps with 0.2 &micro;m hydrophobic membranes are the standard for CO₂ incubator use. The 0.2 &micro;m pore size blocks microbial contamination while allowing CO₂ and O₂ to equilibrate freely, maintaining medium pH through the bicarbonate buffering system. Plug seal caps isolate the flask from the ambient atmosphere &mdash; used for short-term transport, sealed storage, and anaerobic culture applications. Some protocols require alternating between vent and plug seal during different culture phases. Sterility and Traceability All NEST Scientific cell culture flasks carry E-beam sterilization (ISO 11137 equivalent SAL 10⁻⁶), DNase/RNase-free and pyrogen-free lot certification, batch traceability codes, and are produced under ISO 9001:2015 and ISO 13485 quality management. Flasks are individually packaged in peelable Tyvek sleeves or resealable sterile bags. Each flask features two large labeling areas for cell line identification and passage number notation.
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<h2 dir="ltr">Compare Cell Culture Flask Sizes </h2>
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<li>T25: Growth Area: 25 cm&sup2;; Working Volume: 5&ndash;10 mL; Typical Cell Yield (adherent): 5&ndash;8 &times; 10⁶; Best Application: Seed stock, new cell line setup.</li>
<li>T75: Growth Area: 75 cm&sup2;; Working Volume: 15&ndash;25 mL; Typical Cell Yield (adherent): 15&ndash;25 &times; 10⁶; Best Application: Routine passage and maintenance.</li>
<li>T175: Growth Area: 175 cm&sup2;; Working Volume: 35&ndash;50 mL; Typical Cell Yield (adherent): 35&ndash;60 &times; 10⁶; Best Application: Scale-up, transient transfection.</li>
<li>T225: Growth Area: 225 cm&sup2;; Working Volume: 50&ndash;70 mL; Typical Cell Yield (adherent): 50&ndash;80 &times; 10⁶; Best Application: High-yield expansion, virus production.</li>
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<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific cell culture flasks (T25 catalog 707001, T75 catalog 708001, T175 catalog 709001, T225 catalog 710001) are manufactured from 100% virgin polystyrene in an ISO 8 (Class 10,000) cleanroom. Vacuum plasma TC treatment creates a surface contact angle of &lt;10&deg; (hydrophilic) per NEST lot data, verified by water contact angle goniometry. Vented caps contain a hydrophobic 0.2 &micro;m PTFE membrane that passes the USP Particulate Standard for injectable preparations &mdash; confirmed free of fibers and particulates at lot release. Flask footprints are standard for incubator shelf spacing: T25 width 100 mm, T75 width 150 mm, T175/T225 width 167 mm. As of 2026, NEST Scientific flasks are stocked by MBP for same-case reorder by PO. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center. 
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Eliminate variables in the incubator to ensure absolute integrity in your results with MBP's Cell Culture Flasks! <a href="https://mbpinc.net/contact-us">Reach out to our team</a> today for volume-based pricing.

<h2 dir="ltr">What are TC-Treated Cell Culture Flasks?</h2>
 Tissue culture&ndash;treated (TC-treated) cell culture flasks are sterile polystyrene T-flasks whose inner growth surface has been modified by vacuum plasma treatment to introduce negatively charged, oxygen-containing functional groups (carboxyl, carbonyl, hydroxyl) that reduce the water contact angle from ~75&ndash;90&deg; (hydrophobic, untreated PS) to below 10&deg; (hydrophilic). This surface modification directly promotes cell attachment, spreading, and cytoskeletal organization in adherent mammalian cell lines. Without TC treatment, virtually all primary mammalian cells and established adherent lines, including HeLa, HEK293 (adherent), CHO-K1, Vero, A549, NIH 3T3, MDCK, and primary fibroblasts, fail to attach and undergo anoikis within 24&ndash;48 hours. TC-treated flasks are the correct default for any adherent mammalian cell line unless the experimental protocol explicitly specifies non-treated or specialized surfaces (ultra-low attachment, poly-D-lysine, collagen I).
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<h2 dir="ltr">What you will find:</h2>
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TC-Treated Surfaces: Vacuum-plasma modified polystyrene for maximum cellular adhesion and uniform growth kinetics.
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Gas Exchange Efficiency: Vented caps with 0.22&micro;m hydrophobic membranes ensure sterile CO2 exchange while preventing contamination.
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Scalable Geometry: Available in T25, T75, T175, and T225 formats to support both routine maintenance and high-yield production.
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Advanced Ergonomics: The angled neck design facilitates complete access for cell scrapers and serological pipettes, minimizing media waste.
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Optical Excellence: USP Class VI high-clarity material provides distortion-free microscopy for precise monitoring.
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Certified Purity: Validated RNase, DNase, and Pyrogen-free to ensure experimental data integrity.
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<h2 dir="ltr"> How to Choose TC-Treated Cell Culture Flasks</h2>
 Size by Growth Area and Workflow Stage T25 (25 cm&sup2;, 5&ndash;10 mL): seed stocks, low-passage critical cell lines, newly thawed cells, and experiments requiring fewer than ~8 &times; 10⁶ cells. T75 (75 cm&sup2;, 15&ndash;25 mL): the standard research-lab flask for routine weekly passage of most adherent cell lines; the most commonly stocked format. T175 (175 cm&sup2;, 35&ndash;50 mL): scale-up for transient transfection (lipofection, calcium phosphate, PEI), viral vector production (lentivirus, AAV), and protein expression experiments requiring 35&ndash;60 &times; 10⁶ adherent cells per flask. T225 (225 cm&sup2;, 50&ndash;70 mL): maximum single-flask density before transitioning to multi-layer BioFactory vessels; used for large-scale CHO adherent culture and high-yield antibody or virus production runs. Vented vs. Plug Seal Cap Vented caps with a 0.2 &micro;m hydrophobic PTFE membrane allow free CO₂/O₂ gas exchange &mdash; mandatory for cells buffered by the bicarbonate/CO₂ system in a CO₂ incubator (typically 5&ndash;10% CO₂). Plug seal caps create a sealed environment; use them for short-term transport between incubators, for HEPES-buffered media that do not require CO₂, or for anaerobic culture. Using a plug seal cap in a standard CO₂ incubator blocks gas exchange and acidifies the medium within 2&ndash;4 hours. Straight vs. Canted Neck Straight-neck flasks are the traditional geometry; canted (angled) neck flasks tilt the growth surface away from the neck, improving pipette access during media changes and cell harvesting. NEST Scientific uses a canted neck design across all flask sizes. Confirm your biosafety cabinet arm reach and pipette length before committing to one geometry in bulk. Lot-to-Lot Consistency For long-running experiments, drug screening, or validated bioprocess workflows, lot-to-lot TC treatment consistency is critical. NEST Scientific verifies contact angle on each manufacturing lot. Switching the flask lots mid-experiment can alter cell attachment density, proliferation rate, and morphology. Order sufficient flasks from a single lot for the duration of a defined experimental series. Cells That Require TC-Treated Surfaces HeLa, A549, Vero (BSC-1), HEK293 (adherent), CHO-K1 (monolayer), MDCK, NIH 3T3, MCF-7, U2OS, PC-3, HepG2, primary dermal fibroblasts, primary endothelial cells, primary epithelial cells, and most primary culture preparations require TC-treated surfaces. Cells that must NOT be cultured on TC-treated surfaces include Jurkat, K562, HL-60, suspension CHO-S, hybridomas, Sf9/Hi5 insect cells, and hematopoietic progenitor cells &mdash; use non-treated flasks for all of these. 
<h2 dir="ltr">Compare TC-Treated Flask SKUs</h2>
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TC-Treated T25, vent cap: Growth Area: 25 cm&sup2;; Working Volume: 5&ndash;10 mL; NEST Catalog: 707001; Typical Adherent Yield: 5&ndash;8 &times; 10⁶ cells.
TC-Treated T75, vent cap: Growth Area: 75 cm&sup2;; Working Volume: 15&ndash;25 mL; NEST Catalog: 708001; Typical Adherent Yield: 15&ndash;25 &times; 10⁶ cells.
TC-Treated T175, vent cap: Growth Area: 175 cm&sup2;; Working Volume: 35&ndash;50 mL; NEST Catalog: 709001; Typical Adherent Yield: 35&ndash;60 &times; 10⁶ cells.
TC-Treated T225, vent cap: Growth Area: 225 cm&sup2;; Working Volume: 50&ndash;70 mL; NEST Catalog: 710001; Typical Adherent Yield: 50&ndash;80 &times; 10⁶ cells.
TC-Treated T75, plug seal: Growth Area: 75 cm&sup2;; Working Volume: 15&ndash;25 mL; NEST Catalog: 708002; Typical Adherent Yield: 15&ndash;25 &times; 10⁶ cells
 Cell yields are approximate for standard adherent lines at confluence; actual yields vary by cell type, seeding density, and passage number. 
<h2 dir="ltr"> Specifications Context </h2>
 NEST Scientific TC-treated flasks are manufactured from 100% virgin polystyrene in ISO 8 (Class 10,000) cleanrooms. Vacuum plasma TC treatment is verified by water contact angle goniometry per manufacturing lot; contact angle &lt;10&deg; is the release criterion. E-beam sterilization achieves SAL 10⁻⁶; flasks are DNase/RNase-free and non-pyrogenic per lot certificate. Vented cap 0.2 &micro;m PTFE hydrophobic membrane is certified free of particles per USP Particulate Standard. Flask footprint dimensions comply with standard CO₂ incubator shelf spacing: T25 (100 mm width), T75 (150 mm), T175/T225 (167 mm). Double-layered Tyvek packaging maintains sterility until the point of use. NEST Scientific holds ISO 9001:2015, ISO 13485, and ISO 11137 certifications, and has FDA registration and CE marking. As of 2026, MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center.
 <a href="https://mbpinc.net/contact-us">Connect with the helpful team at MBP </a>today to secure a customized quote for your facility.

Treated Cell Culture Flasks

<h2 dir="ltr">What are Non-Treated Cell Culture Flasks?</h2>
 Non-treated cell culture flasks are sterile polystyrene T-flasks that retain the original hydrophobic, unmodified surface of the polystyrene substrate &mdash; no plasma, corona, or chemical surface treatment is applied. The hydrophobic surface minimizes cell attachment, making these flasks the correct vessel for cell types that grow in suspension or require minimal surface contact: suspension-adapted CHO, Jurkat, K562, HL-60, hematopoietic progenitors, hybridomas for monoclonal antibody production, insect cell lines (Sf9, Hi5), yeast, and bacteria. NEST Scientific non-treated flasks are manufactured from 100% virgin polystyrene in ISO 8 cleanrooms, E-beam sterilized to SAL 10⁻⁶, and available in T25, T75, T175, and T225 sizes with vented (0.2 &micro;m PTFE membrane) or plug seal caps. 
Use non-treated flasks exclusively for cell types that do not require surface attachment; placing adherent mammalian cell lines in a non-treated flask causes cell death within 24&ndash;48 hours. 
<h2 dir="ltr">What you will find:</h2>
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Suspension-Optimized Surface, like naturally hydrophobic polystyrene, ensures cells remain in suspension without surface interference.
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Scalable Formats precision-engineered in T25, T75, T175, and T225 sizes to fit various research scales.
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Aseptic Security with certified RNase, DNase, and Pyrogen-free to maintain the highest purity standards.
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Ergonomic Access with angled neck design facilitates seamless pipette access and reduces the risk of contamination during media changes.
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<h2 dir="ltr">How to Choose Non-Treated Cell Culture Flasks</h2>
 Application: Suspension Mammalian Cells Suspension-adapted cell lines &mdash; CHO-S, HEK293-F, Expi293, and HEK293-EBNA variants used in transient transfection &mdash; require non-treated flasks for static suspension culture between spinner flask passages or when the scale is too small for orbital shaker flasks. Cells must be agitated (shaken, pipetted, or rocked) periodically to prevent settling when in static non-treated flasks with volumes &gt;5 mL. Application: Hematopoietic and Immune Cells Non-adherent hematopoietic cell lines (Jurkat T-cells, K562 erythroleukemia, HL-60 promyelocytes, Raji B-cells) and primary hematopoietic cells (peripheral blood mononuclear cells (PBMCs), bone marrow progenitors) must be cultured in non-treated vessels. TC-treated surfaces activate integrin signaling in hematopoietic cells, inducing undesired differentiation and stress responses. Application: Hybridoma Culture Hybridoma cells for monoclonal antibody (mAb) production are non-adherent B-cell fusions that grow as suspension cultures. Non-treated T75 and T225 flasks, combined with HT-supplemented DMEM/HAT medium, are the standard vessels for hybridoma selection and expansion. Scale-up from T225 non-treated flasks typically progresses to spinner or hollow-fiber bioreactors. Application: Insect Cells Sf9 (Spodoptera frugiperda) and Hi5 (Trichoplusia ni) insect cells used in baculovirus expression vector systems (BEVS) require non-treated polystyrene for monolayer culture. On TC-treated surfaces, Sf9 cells attach so strongly that they are difficult to recover. Non-treated flasks allow Sf9 to loosely adhere and detach easily with tapping, or to grow as a loosely attached monolayer that tolerates trypsin-free passaging. Application: Microbial Culture Non-treated polystyrene T-flasks with plug seal caps can serve as culture vessels for E. coli, Bacillus subtilis, yeast (S. cerevisiae, P. pastoris), and other microorganisms where the large surface area per volume is needed, and standard Erlenmeyer flask orbital shaking is unavailable. Ventilated caps support aerobic culture; confirm that the vented 0.2 &micro;m membrane is compatible with your aeration requirements. Cap Selection for Non-Treated Flasks Vented caps (0.2 &micro;m PTFE membrane) are standard for suspension cultures in a CO₂ incubator. For microbial aerobic culture without a CO₂ incubator, vented caps with 0.2 &micro;m pore still provide adequate aeration. Plug seal caps are used for anaerobic culture, transport, and short-term storage. 
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<h2 dir="ltr">Compare Non-Treated Flask SKUs </h2>
Non-treated T25, vent cap: Growth Area: 25 cm&sup2;; Working Volume: 5&ndash;10 mL; NEST Catalog: 707003; Application: Small-scale suspension culture, seed stock.
Non-treated T75, vent cap: Growth Area: 75 cm&sup2;; Working Volume: 15&ndash;25 mL; NEST Catalog: 708003; Application: Routine suspension maintenance.
Non-treated T175, vent cap: Growth Area: 175 cm&sup2;; Working Volume: 35&ndash;50 mL; NEST Catalog: 709003; Application: Scale-up, CHO transfection, hybridoma.
Non-treated T225, vent cap: Growth Area: 225 cm&sup2;; Working Volume: 50&ndash;70 mL; NEST Catalog: 710003; Application: High-yield suspension expansion.
Non-treated T75, plug seal: Growth Area: 75 cm&sup2;; Working Volume: 15&ndash;25 mL; NEST Catalog: 708004; Application: Anaerobic, transport, sealed storage.
<h2> Specifications Context</h2>
 NEST Scientific non-treated cell culture flasks are manufactured from 100% virgin polystyrene with no surface modification. Contact angle of non-treated PS surface is approximately 75&ndash;90&deg; (hydrophobic), confirmed by water contact angle measurement per NEST lot QC data. E-beam sterilized in ISO 8 cleanrooms to SAL 10⁻⁶; DNase-free, RNase-free, and non-pyrogenic per lot certificate. Vented caps use a 0.2 &micro;m hydrophobic PTFE membrane. As of 2026, NEST Scientific non-treated flasks are stocked by MBP in all four sizes (T25, T75, T175, T225) with vented and plug seal options, in case quantities for PO-based institutional ordering. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center. 
Elevate your suspension culture workflows. <a href="https://mbpinc.net/contact-us">Contact Molecular Biology Products Inc.</a> for your Non-Treated Cell Culture Flasks today!

Non-Treated Cell Culture Flasks

DataSheet

Product Literature

T150 U-Shaped Canted Neck Cell Culture Flask Specifications

T150 U-Shaped Canted Neck Cell Culture Flask

A brand new specification, T150 U-shaped Canted Neck Cell Culture Flask, is added to the collection, which deviates from the regular design. Without influencing the regular usage, the bottleneck is enlarged, allowing the pipettes or cell scrapers to reach every corner of the flask freely and flexibly. The unique U-shaped body is easier to hold and enhances the utilization rate of the bottom.

T150 U-Shaped Canted Neck Cell Culture Flask

SKU : 720001