Cell Culture

BioFactory Accessories

<h2 dir="ltr"> What are Accessories for NEST BioFactory&trade; Systems?</h2>
 NEST BioFactory&trade; accessories are the replacement caps, silicone tubing, vent filters, overcaps, and pre-assembled closed-system cap assemblies used to configure BioFactory units for closed aseptic liquid transfer, gas exchange management, and multi-unit serial connections in cGMP and research-scale bioprocess workflows. The accessory set enables the BioFactory narrow-mouth cap to function as a closed-system liquid transfer port &mdash; media, wash buffer, trypsin, and cell harvest supernatant are transferred in and out through the silicone tubing connection without opening the vessel. Accessories for the BioFactory are required when operating the BioFactory as a closed system; for simple open-pour workflows using wide-mouth caps, standard vent and plug seal caps are the only accessories needed.
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<h2 dir="ltr">What you will find:</h2>
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BioFactory media transfer caps
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Sterile tubing and connector sets
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Fluid transfer adapters for cell culture vessels
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BioFactory bottle caps and closures
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Sterile quick-connect systems
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Liquid handling accessories
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Vessel handling and support tools
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Closed-system connectors for aseptic processing
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<h2 dir="ltr"> Key BioFactory Accessory Categories</h2>
 BioFactory Cap with Silicone Tube (CPC or Luer Connector) The BioFactory cap with silicone tube is the primary closed-transfer accessory: an adaptor cap that fits the narrow-mouth port of the BioFactory unit, pre-assembled with 80 cm of silicone tubing and a terminal connector &mdash; either a male CPC (clamped pressure connector / quick-connect) or a standard Luer lock &mdash; for coupling to peristaltic pumps, other BioFactory units, centrifuge tubes, or PETG media bottle assemblies. Two tubing I.D. &times; O.D. configurations are available: 1/4" I.D. &times; 3/8" O.D. and 3/8" I.D. &times; 5/8" O.D., covering standard peristaltic pump head bore sizes. The female sealing cap included with each assembly caps the connector between uses, maintaining sterility. Overcaps: Vented and Solid Overcaps are secondary dust and contamination barriers placed directly on top of the BioFactory adaptor caps during culture incubation. The vented overcap contains a 0.22 &micro;m hydrophobic breathable membrane, allowing gas exchange through the cap stack while blocking particles and microbial contamination &mdash; used during active shaker or orbital incubation when the unit's primary cap is an adaptor cap rather than a vented cap. The solid overcap provides an additional dust and contamination seal with no gas exchange &mdash; used for short-term storage or transport outside the incubator. SPT-50 Silicone Hose SPT-50 silicone hose (3/8" I.D. &times; 5/8" O.D., 50 inches / 15 meters) is the bulk tubing used to connect multiple BioFactory units in series, connect BioFactory ports to peristaltic pump heads, or extend the reach of the transfer cap assembly. SPT-50 tubing is manufactured from silicone, is individually wrapped and sterile, and passes dissolution testing per BPOG (BioPhorum Operations Group) criteria per NEST product documentation, supporting its use in biopharmaceutical production environments. Premium Accessory Package NEST offers a BioFactory Accessory Premium Package that includes 2 different adaptor caps, 1 hose clamp, 1 adaptor connector, 1 vent filter (50 mm), 1 additional hose clamp, and 15 m of SPT-50 silicone hose &mdash; all individually wrapped and sterile in a single kit. This package provides all components needed to set up a closed-system BioFactory media management workflow from a single kit, reducing the number of individual part orders required. Vent Filters for Gas Exchange Management For BioFactory units using adaptor caps rather than standard vented caps, 50 mm inline vent filters (0.22 &micro;m hydrophobic PTFE membrane) are installed on the air inlet/outlet tubing line to maintain sterile gas exchange during media addition, harvest, and incubation. The vent filter is rated for high flow rates without creating excessive back-pressure that would resist liquid movement through the system. Compatibility: Narrow-Mouth BioFactory Units Only All BioFactory accessory caps and tubing assemblies are designed exclusively for the narrow-mouth BioFactory configuration. Wide-mouth BioFactory units use standard wide-mouth vent and plug seal caps only; they are not compatible with accessory cap assemblies. Confirm that your BioFactory units have narrow-mouth ports before ordering the accessory cap set. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST BioFactory&trade; accessory silicone tubing and cap assemblies are manufactured from USP Class VI silicone (TPE/silicone) and polycarbonate or PP cap bodies, individually wrapped and sterile. All components have passed dissolution testing per BPOG dissolution test criteria conducted by a certified third-party laboratory per NEST Scientific documentation. The standard Luer head connections conform to ISO 594 / GB/T 1962 for reliable compatibility with standard laboratory fittings. Vent filters use a 0.22 &micro;m hydrophobic PTFE membrane; overcaps use a 0.22 &micro;m hydrophobic membrane. NEST BioFactory accessories are carried by MBP alongside BioFactory units. 
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Need the right BioFactory setup? <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact MBP today</a> for technical assistance.

Biofactory System Accessories

Product Literature

BioFactory Holder, Individually Wrapped, 1/pk, 5/cs

751101 by NEST Scientific is a BioFactory holder designed to support and stabilize NEST BioFactory multi-chamber cell culture systems in the incubator, during media exchange, and in transport. Individually wrapped to maintain surface cleanliness before use. Pack size: 1/pk, 5 packs per case.

Manufacturer

Vacuum Filtration

<h2 dir="ltr">What are Vacuum Bottle-Top Filter Systems?</h2>
 Vacuum bottle-top filter systems are single-use, sterile filtration assemblies consisting of a graduated polystyrene funnel with an integrated membrane filter bonded to a polystyrene receiver bottle, connected by a hose-port adaptor to a house vacuum line or benchtop vacuum pump. The applied negative pressure (&minus;50 to &minus;100 kPa) draws liquid through the membrane into the receiver bottle, which is sealed after filtration for immediate use or cold-room storage.&nbsp;
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<h2 dir="ltr">What you will find:</h2>
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50mL Vacuum Filtration Units for small-scale sterile sampling
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150mL Vacuum Filtration Systems for rapid reagent preparation
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250mL Filtration Units for routine buffer sterilization
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500mL Vacuum Filtration Cups for mid-scale media processing
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1000mL Large Volume Filtration for industrial-scale batching
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Sterile Bottle-Top Filters for secure aseptic transfer
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<h2 dir="ltr"> How to Choose Vacuum Filtration Systems</h2>
 PES vs. PVDF Membrane for Vacuum Filtration PES membranes provide low protein binding (~1&ndash;2 &micro;g/cm&sup2;), high flow rates, and broad aqueous-solution compatibility &mdash; the standard for routine DMEM, RPMI, HBSS, PBS, and supplement-containing media sterilization. PVDF membranes have slightly lower protein binding than PES and greater chemical resistance to mild organic solvents, mild acids, and alkaline solutions. Use PVDF when filtering protein-rich solutions (FBS, antibody preparations, conditioned medium with high protein content) or when mild organic solvents are present in the filter solution. Volume Selection 250 mL systems (50 mm diameter membrane, 50 mL funnel) suit small media aliquots, supplement solutions, and transfection reagent preparations. 500 mL systems (90 mm diameter membrane, 150 mL funnel) are the routine lab workhorse for standard 500 mL media bottle preparation and FBS filtration. 1,000 mL systems (90 mm diameter membrane, 350 mL funnel) handle full-liter media preparations and large-volume buffer sterilization. All NEST systems include a pre-attached, graduated polystyrene receiver bottle in the corresponding volume. Pore Size: 0.10 &micro;m vs. 0.22 &micro;m vs. 0.45 &micro;m 0.22 &micro;m is the sterilizing grade per USP &lt;1229.2&gt; and ICH Q9: retains all bacteria and fungi; used for routine cell culture media, PBS, and buffer preparation. 0.45 &micro;m is the clarification grade: used as a pre-filtration step before 0.22 &micro;m to remove large particulates from turbid or high-biomass samples (FBS, conditioned media, cell culture supernatants, crude lysates), extending 0.22 &micro;m filter throughput by 3&ndash;5&times; on difficult samples. 0.10 &micro;m is the mycoplasma-removal grade: required when preparing media for Mycoplasma-sensitive primary cell culture or when processing serum or supplementary reagents that may harbor Mycoplasma species. Note that 0.10 &micro;m filtration is substantially slower than 0.22 &micro;m due to the tighter membrane pore structure. Practical Tips for Maximum Throughput Pre-wet the membrane by briefly passing 5&ndash;10 mL of sterile water through before the sample &mdash; this wets the hydrophilic PES or PVDF membrane and reduces initial resistance. For FBS and other viscous solutions, pre-filter through 0.45 &micro;m before the 0.22 &micro;m step. Maintain a vacuum at &minus;50 to &minus;80 kPa; excessive vacuum (&gt; &minus;100 kPa) can shear fragile membrane pores and increase non-specific protein adsorption. Do not autoclave the assembled bottle-top filter &mdash; PES and PS components are not rated for autoclave sterilization. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific vacuum bottle-top filters are manufactured from polystyrene funnel and receiver bottle components with PES or PVDF membranes. PES membranes have documented low protein binding (~1&ndash;2 &micro;g/cm&sup2;) per NEST scientific USA FAQ. Membrane diameter: 250 mL volume &rarr; 50 mm; 500 mL and 1,000 mL &rarr; 90 mm. The hose connector design accommodates various standard vacuum hose diameters. NEST PES 0.22 &micro;m vacuum filters are described as comparable in performance to leading brands, including Corning Stericup, Thermo Fisher Scientific, and Sartorius, per NEST Scientific USA's product page. All NEST vacuum filters are sterile and non-pyrogenic per the lot certificate. NEST Scientific vacuum bottle-top filters are stocked by MBP in all core SKUs. 
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Because your research should move faster than a slow-dripping filter. <a href="https://mbpinc.net/contact-us" title="Contact the Molecular Biology Products Inc. team" style="color: #236fa1;">Contact the Molecular Biology Products Inc. team</a> for a quote before your deadlines catch up with you.

Datsheet

Vacuum Filtration Systems  (PES Membrane)

Bottle Top Vacuum Filter, PES, Sterile

347012 by NEST is a 50 mL bottle top vacuum filter with a 0.1 micrometer hydrophilic PES membrane (50mm diameter), E-beam sterilized to SAL 10^-6, with low protein binding and high flow rate. PS cup and cap; ABS neck; max 45 degrees C working temperature. Not for chloroform, esters, or strong acids/bases. Supplied 1/sterile bag, 24/case.

50 mL Bottle Top Vacuμm Filter, 0.1 μm, PES, Sterile, 1/pk, 24/cs

50 mL Bottle Top Vacuum Filter, 0.22 μm, PES, Sterile,1/pk, 24/cs

50 mL Bottle Top Vacuμm Filter, 0.45 μm, PES, Sterile, 1/pk, 24/cs

150 mL Bottle Top Vacuμm Filter, 0.1 μm, PES, Sterile, 1/pk, 24/cs

150 mL Bottle Top Vacuum Filter, 0.22 μm, PES, Sterile,1/pk, 24/cs

150 mL Bottle Top Vacuμm Filter, 0.45 μm, PES, Sterile, 1/pk, 24/cs

250 mL Bottle Top Vacuμm Filter, 0.1 μm, PES, Sterile, 1/pk, 24/cs

250 mL Bottle Top Vacuum Filter, 0.22 μm, PES, Sterile,1/pk, 24/cs

250 mL Bottle Top Vacuμm Filter, 0.45 μm, PES, Sterile, 1/pk, 24/cs

500 mL Bottle Top Vacuμm Filter, 0.1 μm, PES, Sterile, 1/pk, 24/cs

500 mL Bottle Top Vacuum Filter, 0.22 μm, PES, Sterile,1/pk, 24/cs

500 mL Bottle Top Vacuμm Filter, 0.45 μm, PES, Sterile, 1/pk, 24/cs

1000 mL Bottle Top Vacuμm Filter, 0.1 μm, PES, Sterile, 1/pk, 24/cs

1000 mL Bottle Top Vacuum Filter, 0.22 μm, PES, Sterile,1/pk, 24/cs

1000 mL Bottle Top Vacuμm Filter, 0.45 μm, PES, Sterile, 1/pk, 24/cs

Vacuum Filtration Systems (PVDF Membrane)

Bottle Top Vacuum Filter, PVDF, Sterile

347112 by NEST Scientific is a sterile 50 mL bottle top vacuum filter with a 0.10 µm PVDF membrane, E-beam sterilized to SAL 10⁻⁶, manufactured in an ISO 7 cleanroom, certified DNase/RNase-free and non-pyrogenic, for protein solution filtration, buffer sterilization, and media preparation.

50 mL Bottle Top Vacuμm Filter, 0.1 μm, PVDF, Sterile, 1/pk, 24/cs

50 mL Bottle Top Vacuum Filter, 0.22μm, PVDF, Sterile,1/pk, 24/cs

50 mL Bottle Top Vacuμm Filter, 0.45 μm, PVDF, Sterile, 1/pk, 24/cs

150 mL Bottle Top Vacuμm Filter, 0.1 μm, PVDF, Sterile, 1/pk, 24/cs

150 mL Bottle Top Vacuum Filter, 0.22μm, PVDF, Sterile,1/pk, 24/cs

150 mL Bottle Top Vacuμm Filter, 0.45μm, PVDF, Sterile, 1/pk, 24/cs

250 mL Bottle Top Vacuμm Filter, 0.1 μm, PVDF, Sterile, 1/pk, 24/cs

250 mL Bottle Top Vacuum Filter, 0.22 μm, PVDF, Sterile,1/pk, 24/cs

250 mL Bottle Top Vacuμm Filter, 0.45 μm, PVDF, Sterile, 1/pk, 24/cs

500 mL Bottle Top Vacuμm Filter, 0.1 μm, PVDF, Sterile, 1/pk, 24/cs

500 mL Bottle Top Vacuum Filter, 0.22 μm, PVDF, Sterile,1/pk, 24/cs

500 mL Bottle Top Vacuμm Filter, 0.45 μm, PVDF, Sterile, 1/pk, 24/cs

1000 mL Bottle Top Vacuμm Filter, 0.1 μm, PVDF, Sterile, 1/pk, 24/cs

1000 mL Bottle Top Vacuum Filter, 0.22 μm, PVDF, Sterile,1/pk, 24/cs

1000 mL Bottle Top Vacuμm Filter, 0.45 μm, PVDF, Sterile, 1/pk, 24/cs

C-Flex Welding Hose, Inner dia# 3/8 Inches(9.5 mm)，Out dia# 5/8 Inches(15.9 mm), 50 Inches/ 15 meters, Individually Wrapped, Sterile, 1/pk, 1/cs

746001 by NEST Scientific is a sterile C-Flex-type TPE welding hose with 3/8″ ID (9.5 mm), 5/8″ OD (15.9 mm), and 15-meter length, individually wrapped for BioFactory bioprocess tubing connections. Designed for sterile tube welding and closed-system fluid transfer in biopharmaceutical and cell culture production workflows. Supplied 1/pk, 1/cs.

CPC Connector(Inner Dia #3/8 ) for Hose to Hose Connecting, Individually Wrapped, Sterile, 1/pk, 5/cs

747001 by NEST Scientific — straight hose-to-hose CPC connector, 3/8&quot; inner diameter, individually wrapped, sterile. For single-use bioprocess tubing assemblies. 1/pk, 5/cs.

CPC Sealing Plug, Individually Wrapped, Sterile, 1/pk, 1/cs

747011 by NEST Scientific is a sterile, individually wrapped CPC sealing plug for NEST BioFactory cell culture vessels, sized for #3/8 inch ports (9.5 mm ID / 15.9 mm OD). Manufactured in ISO 8 clean rooms, USP Class VI materials, ISO 9001/13485 certified. 10 pcs/case. Available through MBP, a registered vendor to Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, Eurofins, and many other institutes across the USA.

Cell Culture Dishes

<h2 dir="ltr"> What are Cell Culture Dishes?</h2>
 Cell culture dishes are sterile, shallow, circular polystyrene containers with a lid for growing adherent or suspension mammalian cells, bacteria, yeast, and microorganisms in a CO₂ incubator or ambient environment. TC-treated (tissue culture&ndash;treated) dishes support adherent mammalian cell monolayer growth; non-treated dishes suit suspension cells, colony counting, and bacteriological applications. Choose the dish diameter based on required cell number and downstream assay volume: 35 mm for small-scale primary culture and microscopy; 60 mm for transfection and mid-scale assays; 100 mm for routine large-scale expansion, protein isolation, and virus production; 150 mm for maximum single-vessel cell yield.
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<h2 dir="ltr">What you will find:</h2>
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<li data-section-id="zaop1j" data-start="0" data-end="134">TC-treated and sterile cell culture dishes in standard, polystyrene (PS), Diamond SureGro, gripping ring, and external grip formats.</li>
<li data-section-id="1tw8fwo" data-start="136" data-end="215">Glass bottom cell culture dishes for microscopy-based cell culture workflows.</li>
<li data-section-id="sr7dgd" data-start="217" data-end="298">Spheroid culture products, including Spheroid Dishes and Spheroid Forming Units.</li>
<li data-section-id="s6nepr" data-start="300" data-end="358">3D Cell Floater Dishes for 3D cell culture applications.</li>
<li data-section-id="4r7o73" data-start="360" data-end="422" data-is-last-node="">Multi-Insert Dishes for multi-insert culture configurations.</li>
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<h2 dir="ltr"> How to Choose Cell Culture Dishes</h2>
 Diameter and Growth Area by Application 35 mm dishes (growth area approximately 8.8 cm&sup2;, working volume 2 mL) are the standard for primary cell culture, colony counting, clonal selection, and high-resolution phase-contrast microscopy from adherent monolayers, where the small diameter matches the field of view of low-magnification objectives. 60 mm dishes (growth area approximately 21 cm&sup2;, working volume 5 mL) suit transfection experiments (calcium phosphate, lipofection, PEI) and mid-scale protein or RNA harvest. 100 mm dishes (growth area approximately 55 cm&sup2;, working volume 10 mL) are the routine format for cell expansion, large-scale transfection (AAV, lentivirus production), and whole-cell protein or nucleic acid extraction. 150 mm dishes (growth area approximately 148 cm&sup2;, working volume 20&ndash;30 mL) provide maximum cell yield from a single vessel for high-yield protein production, ChIP-seq input material, and large-scale virus production. TC-Treated vs. Non-Treated Dishes TC-treated NEST dishes have vacuum plasma&ndash;modified surfaces (contact angle &lt;10&deg;) supporting adherent mammalian cell attachment, equivalent to TC-treated T-flask surfaces. Non-treated dishes (when available) maintain the hydrophobic PS surface for suspension cell maintenance, organoid culture, and bacteriological applications. Confirm surface treatment from the product label before seeding; TC-treated and non-treated dishes are visually identical. Gripping Ring Option for 100 mm Dishes NEST Scientific 100 mm dishes are available with and without an Easy-Grip gripping loop molded into the dish wall. The gripping ring provides a secure handhold through latex or nitrile gloves in a biosafety cabinet, reducing slip-and-drop contamination events during media changes and trypsin addition for large-batch 100 mm dish workflows. For labs handling &gt;20 dishes per day, gripping ring dishes are a practical quality-of-life improvement. Optical Clarity and Microscopy NEST Scientific cell culture dishes are manufactured from 100% virgin polystyrene with a flat, distortion-free growth surface, allowing phase-contrast microscopy and standard fluorescence widefield microscopy directly in the dish without cell transfer. Numeric position marks on the dish bottom allow users to re-locate specific fields between imaging sessions. For confocal, TIRF, or super-resolution imaging requiring No. 1.5 coverglass, use glass-bottom dishes or [glass-bottom cell culture plates](mbpinc.net/consumables/cell-culture/cell-culture-plates/glass-bottom-cell-culture-plates) instead. Stackability and Incubator Efficiency NEST Scientific cell culture dishes feature a raised rim geometry and a snap-fit lid that allow stable stacking in a CO₂ incubator. Stacking feet on the lid prevent the lid from sliding and ensure the inner lid face does not contact the dish rim of the dish below &mdash; maintaining sterility of the lid's inner surface during stacking. For high-throughput cell culture labs running &gt;50 dishes simultaneously, dish stackability significantly increases effective incubator shelf utilization. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific cell culture dishes are manufactured from 100% virgin polystyrene in ISO 8 (Class 10,000) cleanrooms. Vacuum plasma TC treatment is verified by water contact angle goniometry per lot (criterion: contact angle &lt;10&deg;). E-beam sterilized to SAL 10⁻⁶; certified DNase/RNase-free and non-pyrogenic per lot. Flat bottom for distortion-free phase-contrast microscopy; numeric position marks on dish floor. NEST Scientific is ISO 9001:2015, ISO 13485, and ISO 11137 certified with FDA registration and CE marking. As of 2026, NEST Scientific dishes in all four sizes are stocked by MBP. 
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Don&rsquo;t compromise on cultural integrity. <a href="https://mbpinc.net/contact-us">Contact the Molecular Biology Products Inc. team</a> for&nbsp;high-performance labware. Request a quote today to streamline your laboratory workflow.

DataSheet

Cell Culture Dish Specifications

Cell Culture Dish, TC Treated, Sterile

706001 by NEST Scientific — 35 mm TC-treated polystyrene cell culture dish, 9 cm&sup2; growth area, 1.8&ndash;2.7 mL working volume, vented lid, sterile, with numeric position indicators. 20/pk, 500/cs.

35mm Cell Culture Dish, TC, sterile 20/pk, 500/cs

60mm Cell Culture Dish, TC, sterile 20/bag, 500/cs

100mm Cell Culture Dish, TC, sterile 5/bag, 300/cs

100mm Cell Culture Dish, TC, sterile 20/bag, 300/cs

150mm Cell Culture Dish, TC, sterile 5/pk,100/cs

Cell Culture Dish, with Gripping Ring Specifications

Cell Culture Dish, with Gripping Ring, TC Treated, Sterile

The 706201 by NEST Scientific is a 35 mm TC-treated sterile cell culture dish manufactured from USP Grade VI virgin polystyrene in an ISO 7 cleanroom. It delivers 8.5 cm² of growth area, plasma-etched for uniform cell attachment, and features numeric position indicators on the dish bottom for microscopic field relocation. Certified pyrogen-free (USP 85), cytotoxin-free (USP 87/88), DNase/RNase-free, and BSE/TSE-free; sterilized by e-beam to SAL 10⁻⁶. Packed 20 per sleeve, 500 per case.

35mm Cell Culture Dish, with Gripping Ring, TC, Sterile, 20/pk, 500/cs

60mm Cell Culture Dish, with Gripping Ring, TC, Sterile, 20/pk, 500/cs

100mm Cell Culture Dish, with Gripping Ring, TC, Sterile 20/bag, 300/cs

Cell Culture Flasks

<h2 dir="ltr">What are Cell Culture Flasks?</h2>
 Cell culture flasks (T-flasks) are sterile, rectangular polystyrene vessels with a canted, angled neck designed to house adherent or suspension cell cultures in a CO₂ incubator. They are available in growth surface areas from T25 (25 cm&sup2;) to T225 (225 cm&sup2;), with either tissue culture&ndash;treated (TC-treated) surfaces for adherent cells or non-treated surfaces for suspension and microbial culture. Cap options include vented caps with 0.2 &micro;m hydrophobic membranes for CO₂ gas exchange, and plug seal caps for anaerobic or sealed transport.&nbsp; Choose the flask size based on the number of cells needed for downstream applications: T25 for seed stocks, T75 for routine maintenance, T175 and T225 for scale-up and protein production runs.
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<h2 dir="ltr">What you will find:</h2>
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<a href="cell-culture-flasks/treated-cell-culture-flasks" title="Treated Cell Culture Flasks" style="color: #236fa1;">Treated Cell Culture Flasks</a>: Vacuum-plasma modified surfaces that enhance hydrophilic interaction for uniform monolayer formation.
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<a href="cell-culture-flasks/non-treated-cell-culture-flasks" title="Non-Treated Cell Culture Flasks:">Non-Treated Cell Culture Flasks:</a> Naturally hydrophobic polystyrene designed specifically for hybridomas and non-adherent suspension cultures.
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<h2 dir="ltr"> How to Choose Cell Culture Flasks</h2>
 Size by Growth Area and Working Volume T25 flasks (25 cm&sup2;, working volume 5&ndash;10 mL) are used for seed stocks, small-scale experiments, and initial passages of new cell lines where preservation of limited cells is critical. T75 flasks (75 cm&sup2;, 15&ndash;25 mL) are the routine maintenance format for most adherent cell lines in research settings. T175 flasks (175 cm&sup2;, 35&ndash;50 mL) suit scale-up for protein production, viral vector manufacturing, and high-yield transient transfection. T225 flasks (225 cm&sup2;, 50&ndash;70 mL) provide maximum single-flask growth area before scale-up to multi-layer vessels. TC-Treated vs. Non-Treated Surface TC-treated flasks (vacuum plasma treated, contact angle &lt;10&deg;) are essential for adherent mammalian cell lines &mdash; HeLa, HEK293, CHO (adherent), Vero, A549, NIH 3T3, and primary cells, including fibroblasts, epithelial cells, and endothelial cells. Non-treated flasks maintain the original hydrophobic PS surface, preventing adherent growth &mdash; used for suspension CHO, Jurkat, K562, hematopoietic cells, hybridomas, Sf9/Hi5 insect cells, and bacterial culture. Canted vs. Straight Neck A canted (angled) neck on the flask allows the flask to rest at an angle when standing, keeping the growth surface slightly tilted during feeding and pipetting. This angled geometry improves access for serological pipettes and reduces the risk of contaminating the culture surface during media changes. All NEST Scientific T-flasks use a canted neck design. Cap Type and Gas Exchange Vented caps with 0.2 &micro;m hydrophobic membranes are the standard for CO₂ incubator use. The 0.2 &micro;m pore size blocks microbial contamination while allowing CO₂ and O₂ to equilibrate freely, maintaining medium pH through the bicarbonate buffering system. Plug seal caps isolate the flask from the ambient atmosphere &mdash; used for short-term transport, sealed storage, and anaerobic culture applications. Some protocols require alternating between vent and plug seal during different culture phases. Sterility and Traceability All NEST Scientific cell culture flasks carry E-beam sterilization (ISO 11137 equivalent SAL 10⁻⁶), DNase/RNase-free and pyrogen-free lot certification, batch traceability codes, and are produced under ISO 9001:2015 and ISO 13485 quality management. Flasks are individually packaged in peelable Tyvek sleeves or resealable sterile bags. Each flask features two large labeling areas for cell line identification and passage number notation.
<h2 dir="ltr">&nbsp;</h2>
<h2 dir="ltr">Compare Cell Culture Flask Sizes </h2>
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<ul>
<li>T25: Growth Area: 25 cm&sup2;; Working Volume: 5&ndash;10 mL; Typical Cell Yield (adherent): 5&ndash;8 &times; 10⁶; Best Application: Seed stock, new cell line setup.</li>
<li>T75: Growth Area: 75 cm&sup2;; Working Volume: 15&ndash;25 mL; Typical Cell Yield (adherent): 15&ndash;25 &times; 10⁶; Best Application: Routine passage and maintenance.</li>
<li>T175: Growth Area: 175 cm&sup2;; Working Volume: 35&ndash;50 mL; Typical Cell Yield (adherent): 35&ndash;60 &times; 10⁶; Best Application: Scale-up, transient transfection.</li>
<li>T225: Growth Area: 225 cm&sup2;; Working Volume: 50&ndash;70 mL; Typical Cell Yield (adherent): 50&ndash;80 &times; 10⁶; Best Application: High-yield expansion, virus production.</li>
</ul>
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific cell culture flasks (T25 catalog 707001, T75 catalog 708001, T175 catalog 709001, T225 catalog 710001) are manufactured from 100% virgin polystyrene in an ISO 8 (Class 10,000) cleanroom. Vacuum plasma TC treatment creates a surface contact angle of &lt;10&deg; (hydrophilic) per NEST lot data, verified by water contact angle goniometry. Vented caps contain a hydrophobic 0.2 &micro;m PTFE membrane that passes the USP Particulate Standard for injectable preparations &mdash; confirmed free of fibers and particulates at lot release. Flask footprints are standard for incubator shelf spacing: T25 width 100 mm, T75 width 150 mm, T175/T225 width 167 mm. As of 2026, NEST Scientific flasks are stocked by MBP for same-case reorder by PO. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center. 
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Eliminate variables in the incubator to ensure absolute integrity in your results with MBP's Cell Culture Flasks! <a href="https://mbpinc.net/contact-us">Reach out to our team</a> today for volume-based pricing.

<h2 dir="ltr">What are Non-Treated Cell Culture Flasks?</h2>
 Non-treated cell culture flasks are sterile polystyrene T-flasks that retain the original hydrophobic, unmodified surface of the polystyrene substrate &mdash; no plasma, corona, or chemical surface treatment is applied. The hydrophobic surface minimizes cell attachment, making these flasks the correct vessel for cell types that grow in suspension or require minimal surface contact: suspension-adapted CHO, Jurkat, K562, HL-60, hematopoietic progenitors, hybridomas for monoclonal antibody production, insect cell lines (Sf9, Hi5), yeast, and bacteria. NEST Scientific non-treated flasks are manufactured from 100% virgin polystyrene in ISO 8 cleanrooms, E-beam sterilized to SAL 10⁻⁶, and available in T25, T75, T175, and T225 sizes with vented (0.2 &micro;m PTFE membrane) or plug seal caps. 
Use non-treated flasks exclusively for cell types that do not require surface attachment; placing adherent mammalian cell lines in a non-treated flask causes cell death within 24&ndash;48 hours. 
<h2 dir="ltr">What you will find:</h2>
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Suspension-Optimized Surface, like naturally hydrophobic polystyrene, ensures cells remain in suspension without surface interference.
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Scalable Formats precision-engineered in T25, T75, T175, and T225 sizes to fit various research scales.
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Aseptic Security with certified RNase-, DNase-, and Pyrogen-free to maintain the highest purity standards.
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Ergonomic Access with angled neck design facilitates seamless pipette access and reduces the risk of contamination during media changes.
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<h2 dir="ltr">How to Choose Non-Treated Cell Culture Flasks</h2>
 Application: Suspension Mammalian Cells Suspension-adapted cell lines &mdash; CHO-S, HEK293-F, Expi293, and HEK293-EBNA variants used in transient transfection &mdash; require non-treated flasks for static suspension culture between spinner flask passages or when the scale is too small for orbital shaker flasks. Cells must be agitated (shaken, pipetted, or rocked) periodically to prevent settling when in static non-treated flasks with volumes &gt;5 mL. Application: Hematopoietic and Immune Cells Non-adherent hematopoietic cell lines (Jurkat T-cells, K562 erythroleukemia, HL-60 promyelocytes, Raji B-cells) and primary hematopoietic cells (peripheral blood mononuclear cells (PBMCs), bone marrow progenitors) must be cultured in non-treated vessels. TC-treated surfaces activate integrin signaling in hematopoietic cells, inducing undesired differentiation and stress responses. Application: Hybridoma Culture Hybridoma cells for monoclonal antibody (mAb) production are non-adherent B-cell fusions that grow as suspension cultures. Non-treated T75 and T225 flasks, combined with HT-supplemented DMEM/HAT medium, are the standard vessels for hybridoma selection and expansion. Scale-up from T225 non-treated flasks typically progresses to spinner or hollow-fiber bioreactors. Application: Insect Cells Sf9 (Spodoptera frugiperda) and Hi5 (Trichoplusia ni) insect cells used in baculovirus expression vector systems (BEVS) require non-treated polystyrene for monolayer culture. On TC-treated surfaces, Sf9 cells attach so strongly that they are difficult to recover. Non-treated flasks allow Sf9 to loosely adhere and detach easily with tapping, or to grow as a loosely attached monolayer that tolerates trypsin-free passaging. Application: Microbial Culture Non-treated polystyrene T-flasks with plug seal caps can serve as culture vessels for E. coli, Bacillus subtilis, yeast (S. cerevisiae, P. pastoris), and other microorganisms where the large surface area per volume is needed, and standard Erlenmeyer flask orbital shaking is unavailable. Ventilated caps support aerobic culture; confirm that the vented 0.2 &micro;m membrane is compatible with your aeration requirements. Cap Selection for Non-Treated Flasks Vented caps (0.2 &micro;m PTFE membrane) are standard for suspension cultures in a CO₂ incubator. For microbial aerobic culture without a CO₂ incubator, vented caps with 0.2 &micro;m pore still provide adequate aeration. Plug seal caps are used for anaerobic culture, transport, and short-term storage.
<h2> Specifications Context</h2>
 NEST Scientific non-treated cell culture flasks are manufactured from 100% virgin polystyrene with no surface modification. Contact angle of non-treated PS surface is approximately 75&ndash;90&deg; (hydrophobic), confirmed by water contact angle measurement per NEST lot QC data. E-beam sterilized in ISO 8 cleanrooms to SAL 10⁻⁶; DNase-free, RNase-free, and non-pyrogenic per lot certificate. Vented caps use a 0.2 &micro;m hydrophobic PTFE membrane. As of 2026, NEST Scientific non-treated flasks are stocked by MBP in all four sizes (T25, T75, T175, T225) with vented and plug seal options, in case quantities for PO-based institutional ordering. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center. 
Elevate your suspension culture workflows. <a href="https://mbpinc.net/contact-us">Contact Molecular Biology Products Inc.</a> for your Non-Treated Cell Culture Flasks today!

Non-Treated Cell Culture Flasks

Cell Culture Flask, Plug Seal Cap, Non-Treated Specifications

Cell Culture Flask, Plug Seal Cap, Non-treated, Sterile - Nest Scientific

707011 by NEST Scientific — 25 cm² T-25 cell culture flask with plug seal cap and non-treated surface. 50 mL total volume, sterile, 10/pk. For suspension cells in CO₂-independent media or transport applications.

25cm2 Cell Culture Flask, Plug Seal Cap, Non-Treated, sterile, Maximum Working Volume 50mL, 10/pk, 200/cs

75cm2 Cell Culture Flask, Plug Seal Cap, Non-Treated, sterile, Maximum Working Volume 250mL, 5/pk, 100/cs

175cm2 Cell Culture Flask, Plug Seal Cap, Non-Treated, sterile, Maximum Working Volume 750mL, 5/pk, 40/cs

225cm2 Cell Culture Flask, Plug Seal Cap, Non-Treated, sterile, Maximum Working Volume 950mL, 5/pk, 25/cs

<h2 dir="ltr">What are TC-Treated Cell Culture Flasks?</h2>
 Tissue culture&ndash;treated (TC-treated) cell culture flasks are sterile polystyrene T-flasks whose inner growth surface has been modified by vacuum plasma treatment to introduce negatively charged, oxygen-containing functional groups (carboxyl, carbonyl, hydroxyl) that reduce the water contact angle from ~75&ndash;90&deg; (hydrophobic, untreated PS) to below 10&deg; (hydrophilic). This surface modification directly promotes cell attachment, spreading, and cytoskeletal organization in adherent mammalian cell lines. Without TC treatment, virtually all primary mammalian cells and established adherent lines, including HeLa, HEK293 (adherent), CHO-K1, Vero, A549, NIH 3T3, MDCK, and primary fibroblasts, fail to attach and undergo anoikis within 24&ndash;48 hours. TC-treated flasks are the correct default for any adherent mammalian cell line unless the experimental protocol explicitly specifies non-treated or specialized surfaces (ultra-low attachment, poly-D-lysine, collagen I).
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<h2 dir="ltr">What you will find:</h2>
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TC-Treated Surfaces: Vacuum-plasma modified polystyrene for maximum cellular adhesion and uniform growth kinetics.
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Gas Exchange Efficiency: Vented caps with 0.22&micro;m hydrophobic membranes ensure sterile CO2 exchange while preventing contamination.
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Scalable Geometry: Available in T25, T75, T175, and T225 formats to support both routine maintenance and high-yield production.
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Advanced Ergonomics: The angled neck design facilitates complete access for cell scrapers and serological pipettes, minimizing media waste.
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Optical Excellence: USP Class VI high-clarity material provides distortion-free microscopy for precise monitoring.
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Certified Purity: Validated RNase, DNase, and Pyrogen-free to ensure experimental data integrity.
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<h2 dir="ltr"> How to Choose TC-Treated Cell Culture Flasks</h2>
 Size by Growth Area and Workflow Stage T25 (25 cm&sup2;, 5&ndash;10 mL): seed stocks, low-passage critical cell lines, newly thawed cells, and experiments requiring fewer than ~8 &times; 10⁶ cells. T75 (75 cm&sup2;, 15&ndash;25 mL): the standard research-lab flask for routine weekly passage of most adherent cell lines; the most commonly stocked format. T175 (175 cm&sup2;, 35&ndash;50 mL): scale-up for transient transfection (lipofection, calcium phosphate, PEI), viral vector production (lentivirus, AAV), and protein expression experiments requiring 35&ndash;60 &times; 10⁶ adherent cells per flask. T225 (225 cm&sup2;, 50&ndash;70 mL): maximum single-flask density before transitioning to multi-layer BioFactory vessels; used for large-scale CHO adherent culture and high-yield antibody or virus production runs. Vented vs. Plug Seal Cap Vented caps with a 0.2 &micro;m hydrophobic PTFE membrane allow free CO₂/O₂ gas exchange &mdash; mandatory for cells buffered by the bicarbonate/CO₂ system in a CO₂ incubator (typically 5&ndash;10% CO₂). Plug seal caps create a sealed environment; use them for short-term transport between incubators, for HEPES-buffered media that do not require CO₂, or for anaerobic culture. Using a plug seal cap in a standard CO₂ incubator blocks gas exchange and acidifies the medium within 2&ndash;4 hours. Straight vs. Canted Neck Straight-neck flasks are the traditional geometry; canted (angled) neck flasks tilt the growth surface away from the neck, improving pipette access during media changes and cell harvesting. NEST Scientific uses a canted neck design across all flask sizes. Confirm your biosafety cabinet arm reach and pipette length before committing to one geometry in bulk. Lot-to-Lot Consistency For long-running experiments, drug screening, or validated bioprocess workflows, lot-to-lot TC treatment consistency is critical. NEST Scientific verifies contact angle on each manufacturing lot. Switching the flask lots mid-experiment can alter cell attachment density, proliferation rate, and morphology. Order sufficient flasks from a single lot for the duration of a defined experimental series. Cells That Require TC-Treated Surfaces HeLa, A549, Vero (BSC-1), HEK293 (adherent), CHO-K1 (monolayer), MDCK, NIH 3T3, MCF-7, U2OS, PC-3, HepG2, primary dermal fibroblasts, primary endothelial cells, primary epithelial cells, and most primary culture preparations require TC-treated surfaces. Cells that must NOT be cultured on TC-treated surfaces include Jurkat, K562, HL-60, suspension CHO-S, hybridomas, Sf9/Hi5 insect cells, and hematopoietic progenitor cells &mdash; use non-treated flasks for all of these. 
<h2 dir="ltr"> Specifications Context </h2>
 NEST Scientific TC-treated flasks are manufactured from 100% virgin polystyrene in ISO 8 (Class 10,000) cleanrooms. Vacuum plasma TC treatment is verified by water contact angle goniometry per manufacturing lot; contact angle &lt;10&deg; is the release criterion. E-beam sterilization achieves SAL 10⁻⁶; flasks are DNase/RNase-free and non-pyrogenic per lot certificate. Vented cap 0.2 &micro;m PTFE hydrophobic membrane is certified free of particles per USP Particulate Standard. Flask footprint dimensions comply with standard CO₂ incubator shelf spacing: T25 (100 mm width), T75 (150 mm), T175/T225 (167 mm). Double-layered Tyvek packaging maintains sterility until the point of use. NEST Scientific holds ISO 9001:2015, ISO 13485, and ISO 11137 certifications, and has FDA registration and CE marking. As of 2026, MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center.
 <a href="https://mbpinc.net/contact-us">Connect with the helpful team at MBP </a>today to secure a customized quote for your facility.

Treated Cell Culture Flasks

Cell Culture Flask, Plug Seal Cap, Treated Specifications

Cell Culture Flask, Plug Seal Cap, TC, Sterile - Nest Scientific

707001 by NEST Scientific is a 25 cm² TC-treated cell culture flask with an HDPE plug seal cap, manufactured from USP Class VI virgin polystyrene. E-beam sterilized to SAL 10⁻⁶, DNase/RNase-free, cGMP compliant (ISO 13485:2016, ISO 9001, ISO 7 cleanroom). Working volume 5–10 mL; maximum 30 mL. Ships 10/bag, 200/case. Vent-cap variant: 707003.

25cm2 Cell Culture Flask, Plug Seal Cap, TC, sterile, Maximum Working Volume 50mL, 10/pk, 200/cs

75cm2 Cell Culture Flask, Plug Seal Cap, TC, sterile, Maximum Working Volume 250mL, 5/pk, 100/cs

175cm2 Cell Culture Flask, Plug Seal Cap, TC, sterile, Maximum Working Volume 750mL, 5/pk, 40/cs

225cm2 Cell Culture Flask, Plug Seal Cap, TC, sterile, Maximum Working Volume 950mL, 5/pk, 25/cs

Cell Culture Flask, Vent Cap, Non-Treated Specifications

Cell Culture Flask, Vent Cap, Non-Treated, Sterile - Nest Scientific

707013 by NEST Scientific — 25 cm² T-25 cell culture flask with vent cap and non-treated surface. 50 mL total volume, sterile, 10/pk. Designed for suspension cells, hybridoma culture, and applications where surface adhesion must be prevented.

25cm2 Cell Culture Flask, Vent Cap, Non-Treated, sterile, Maximum Working Volume 50mL, 10/pk, 200/cs

75cm2 Cell Culture Flask, Vent Cap, Non-Treated, sterile, Maximum Working Volume 250mL, 5/pk, 100/cs

175cm2 Cell Culture Flask, Vent Cap, Non-Treated, sterile, Maximum Working Volume 750mL, 5/pk, 40/cs

225cm2 Cell Culture Flask, Vent Cap, Non-Treated, sterile, Maximum Working Volume 950mL, 5/pk, 25/cs

Cell Culture Flask, Vent Cap, TC Treated Specifications

Cell Culture Flask, Vent Cap, TC, Sterile - Nest Scientific

NEST Scientific 707003 is a 25 cm² TC-treated cell culture flask with HDPE vented cap, made from USP Class VI polystyrene and e-beam sterilized to SAL 10⁻⁶. Working volume 5–10 mL; 200 per case. ISO 13485:2016 and cGMP certified. Order at <a href="https://www.mbpinc.net/quickorder">mbpinc.net/quickorder</a>.

25cm2 Cell Culture Flask, Vent Cap, TC, sterile, Maximum Working Volume 50mL, 10/pk, 200/cs

75cm2 Cell Culture Flask, Vent Cap, TC, sterile, Maximum Working Volume 250mL, 5/pk, 100/cs

175cm2 Cell Culture Flask, Vent Cap, TC, sterile, Maximum Working Volume 750mL, 5/pk, 40/cs

225cm2 Cell Culture Flask, Vent Cap, TC, sterile, Maximum Working Volume 950mL, 5/pk, 25/cs

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