Cell Culture

Erlenmeyer Flasks

<h2 dir="ltr">What are Cell Culture Erlenmeyer Flasks?</h2>
 Cell culture Erlenmeyer flasks (also called shake flasks or conical flasks) are single-use, sterile plastic vessels with a conical body, flat base, and narrow neck used for suspension cell culture on orbital shakers and incubator shakers, microbial culture, and media preparation. Unlike rectangular T-flasks designed for static monolayer culture, Erlenmeyer flasks are engineered for shaking: their wide conical base maximizes liquid surface area for oxygen transfer, while the narrow neck minimizes evaporation and contamination risk. Choose PC Erlenmeyer flasks for autoclavable reuse and high-temperature media preparation; choose PETG for single-use suspension culture and processes requiring BPA-free materials.
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<h2 dir="ltr">What you will find:</h2>
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<a href="erlenmeyer-flasks/petg-erlenmeyer-flasks">PETG Erlenmeyer flasks: </a>strong choice for cell culture applications that need excellent clarity, reliable handling, and convenient single-use performance. They are lightweight, durable, and ideal for shaker culture, media preparation, and general suspension cell workflows.
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<a href="erlenmeyer-flasks/pc-erlenmeyer-flasks">PC Erlenmeyer flasks: </a>built for applications that require greater strength and higher impact resistance. They are well-suited for demanding lab environments, including vigorous shaking, transport, and workflows where added durability is important.
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<li dir="ltr" aria-level="1"><a href="https://mbpinc.net/consumables/cell-culture/erlenmeyer-flasks/erlenmeyer-flask-accessories">Erlenmeyer Flask Accessories: </a>Includes bi-directional, inverted, and multifunctional transfer caps, plus silicon tube assemblies for 2 L, 3 L, 3 L wide-mouth, and 5 L high-efficiency flasks, supporting sterile transfer and closed-system workflows.</li>
</ul>
<h2 dir="ltr"> How to Choose Erlenmeyer Flasks for Cell Culture</h2>
 PC vs. PETG Material PC (polycarbonate) Erlenmeyer flasks are autoclavable at 121 &deg;C (typically 5&ndash;10 cycles), shatter-proof, crystal-clear, and rated from &minus;135 &deg;C to +135 &deg;C. PC is ideal for labs that sterilize media or wash in-house. PC contains BPA. PETG Erlenmeyer flasks are single-use, BPA-free, rated from &minus;80 &deg;C to +60 &deg;C, and cannot be autoclaved. They provide a contamination-free, cleaning-free alternative to glass and PC for high-throughput suspension culture operations. Flat Base vs. Baffled Base Flat-base Erlenmeyer flasks provide gentler swirling for slow-growth cell lines and media mixing. Baffled-base flasks have three or four angled internal baffles that interrupt the swirling flow, increasing turbulence, raising dissolved oxygen (DO) by up to 2&times; compared to flat-base flasks at the same orbital speed, and reducing cell sedimentation. Baffled flasks are the standard for CHO-S, HEK293-F, Sf9, microbial, and yeast cultures requiring high oxygen transfer rates. NEST Scientific offers baffled and flat-base versions in both PC and PETG across all core volumes. Vented vs. Plug Seal Cap Vented caps contain a 0.22 &micro;m hydrophobic PTFE membrane that allows free O₂/CO₂ gas exchange while excluding microbial contamination. The NEST vented cap features an oversized vent area that improves gas exchange beyond standard-size membrane caps, supporting oxygen-demanding suspension cultures at higher cell densities. Plug seal caps create a fully sealed environment for media storage, static incubation, or transport outside the incubator. Always use vented caps for actively growing suspension cultures on an orbital shaker. Volume and Fill Ratio Erlenmeyer flasks should be filled to no more than 20&ndash;25% of the nominal flask volume for suspension shaker culture to maintain adequate headspace for oxygen transfer. A 500 mL flask running at 20% fill holds 100 mL of culture; a 1,000 mL flask holds 200 mL. Overfilling reduces oxygen transfer efficiency and creates foam that blocks the vented cap membrane. For media storage (no shaking), the fill volume can approach 80% of nominal. Shaker Compatibility NEST Scientific Erlenmeyer flasks (125 mL&ndash;3,000 mL) are compatible with standard laboratory orbital shakers, including those from Infors HT, New Brunswick Scientific (Innova), and Thermo Scientific MaxQ. Confirm flask neck O.D. and base diameter against your shaker's flask holder clips or clamp system before ordering large quantities. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific PC Erlenmeyer flasks are manufactured from USP Class VI medical-grade polycarbonate, gamma sterilized per ISO 11137 to SAL 10⁻⁶, and individually wrapped in sterile packaging. PETG Erlenmeyer flasks are E-beam sterilized and certified DNase/RNase-free, pyrogen-free per lot. Vented cap PTFE membranes are 0.22 &micro;m with an oversized vent area that increases gas exchange efficiency vs. standard-diameter membrane caps. NEST Scientific is ISO 9001:2015 and ISO 13485 certified with FDA registration. Flask catalog sizes extend to high-efficiency 2 L and 5 L PC formats (NEST High Efficiency Erlenmeyer Flask line) for production-scale suspension culture. MBP stocks the NEST Scientific Erlenmeyer flask line across all major volumes and configurations. 
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Support accurate mixing and culturing with premium Erlenmeyer flasks designed for modern laboratories. <a href="https://www.mbpinc.net/contact-us" title="Contact the MBP Team" style="color: #236fa1; text-decoration: underline;">Contact the MBP team</a>&nbsp;for a formal quote.

Product Literature

Flyer

High Efficiency Erlenmeyer Flask with Baffles, with Bi-directional Transfer Cap with TPE Tube & Vent Filter

C20122-AZB050A by NEST Scientific is a 2 L autoclavable polycarbonate Erlenmeyer flask with baffles and a pre-installed bi-directional transfer cap (1/8″ TPE tube, 0.2 µm vent membrane) for closed aseptic liquid transfer. E-beam sterilized SAL 10⁻⁶, USP Class VI, non-pyrogenic. Designed for bacterial, yeast, and mammalian shake-flask culture with enhanced oxygen transfer. 4/cs.

Manufacturer

2L High Efficiency Erlenmeyer Flask with Baffles, with Bi-directional Transfer Cap with TPE Tube (50cm 1/8" ID 1/4" OD), Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 4/cs

3L High Efficiency Erlenmeyer Flask with Baffles, with Bi-directional Transfer Cap with TPE Tube (50cm 1/8" ID 1/4" OD), Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 4/cs

(old item # 787081) 5L High Efficiency Erlenmeyer Flask with Large Membrane Size Bi-directional Transfer Cap with TPE Tube (60cm 1/8" ID 1/4" OD), Sterile, 1/pk, 4/cs

High Efficiency Erlenmeyer Flask with Bi-Directional Transfer Cap, TPE Tube, Male CPC Connector & Vent Filter

C10223-DBE060A by NEST Scientific is a 3 L High Efficiency Erlenmeyer Flask pre-assembled with a bi-directional transfer cap, 60 cm TPE tube (1/4" ID × 7/16" OD), Male CPC quick-connect connector with female sealing cap, and 0.22 µm PTFE vent filter (20 cm²). E-beam sterile, SAL 10⁻⁶. Flask included. 1/pk, 4/cs.

3L High Efficiency Erlenmeyer Flask with Bi-Directional Transfer Cap, TPE Tube (60cm 1/4" ID 7/16" OD), Male CPC Connector with Female Sealing Cap, Vent Filter (0.22 μm 20cm²), Sterile, 1/pk, 4/cs

5L High Efficiency Erlenmeyer Flask with Bi-Directional Transfer Cap, TPE Tube (60cm 1/4" ID 7/16" OD), Male CPC Connector with Female Sealing Cap ,Vent Filter (0.22 μm 20cm²), Sterile, 1/pk, 4/cs

High Efficiency Erlenmeyer Flask with Bi-directional Transfer Cap with TPE Tube & Vent Filter

C10123-BZC050A by NEST Scientific — 2L high efficiency Erlenmeyer flask, USP Class VI polycarbonate, with bi-directional transfer cap, TPE tube (50cm, 1/4&quot; ID &times; 3/8&quot; OD), 0.22 &mu;m PTFE vent filter. E-beam sterilized, DNase/RNase-free. 1/pk, 4/cs.

2L High Efficiency Erlenmeyer Flask with Bi-directional Transfer Cap with TPE Tube (50cm 1/4" ID 3/8" OD), Vent Filter (0.22 μm Φ24mm) , Sterile, 1/pk, 4/cs

3L High Efficiency Erlenmeyer Flask with Bi-directional Transfer Cap with TPE Tube (50cm 1/4" ID 3/8" OD), Vent Filter (0.22 μm Φ24mm), , Sterile, 1/pk, 4/cs

5L High Efficiency Erlenmeyer Flask with Bi-directional Transfer Cap with TPE Tube (50cm 1/4" ID 3/8" OD), Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 4/cs

C10222-AZB050A by NEST Scientific — 3 L high efficiency Erlenmeyer flask with bi-directional transfer cap, 50 cm TPE tube (1/8&quot; ID &times; 1/4&quot; OD), 0.22 &mu;m &times; 24 mm vent filter. Sterile, 1/pk, 4/cs.

3L High Efficiency Erlenmeyer Flask with Bi-directional Transfer Cap with TPE Tube (50cm 1/8" ID 1/4" OD), Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 4/cs

5L High Efficiency Erlenmeyer Flask with Bi-directional Transfer Cap, TPE Tube (50cm 1/8" ID 1/4" OD), Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 4/cs

5L High Efficiency Erlenmeyer Flask with Bi-directional Transfer Cap with TPE Tube (90cm 1/8" ID 1/4" OD), Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 4/cs

BioFactory Accessories

<h2 dir="ltr"> What are Accessories for NEST BioFactory&trade; Systems?</h2>
 NEST BioFactory&trade; accessories are the replacement caps, silicone tubing, vent filters, overcaps, and pre-assembled closed-system cap assemblies used to configure BioFactory units for closed aseptic liquid transfer, gas exchange management, and multi-unit serial connections in cGMP and research-scale bioprocess workflows. The accessory set enables the BioFactory narrow-mouth cap to function as a closed-system liquid transfer port &mdash; media, wash buffer, trypsin, and cell harvest supernatant are transferred in and out through the silicone tubing connection without opening the vessel. Accessories for the BioFactory are required when operating the BioFactory as a closed system; for simple open-pour workflows using wide-mouth caps, standard vent and plug seal caps are the only accessories needed.
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<h2 dir="ltr">What you will find:</h2>
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<li dir="ltr" style="color: #000000;" aria-level="1">
BioFactory media transfer caps
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Sterile tubing and connector sets
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Fluid transfer adapters for cell culture vessels
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BioFactory bottle caps and closures
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Sterile quick-connect systems
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Liquid handling accessories
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Vessel handling and support tools
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Closed-system connectors for aseptic processing
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<h2 dir="ltr"> Key BioFactory Accessory Categories</h2>
 BioFactory Cap with Silicone Tube (CPC or Luer Connector) The BioFactory cap with silicone tube is the primary closed-transfer accessory: an adaptor cap that fits the narrow-mouth port of the BioFactory unit, pre-assembled with 80 cm of silicone tubing and a terminal connector &mdash; either a male CPC (clamped pressure connector / quick-connect) or a standard Luer lock &mdash; for coupling to peristaltic pumps, other BioFactory units, centrifuge tubes, or PETG media bottle assemblies. Two tubing I.D. &times; O.D. configurations are available: 1/4" I.D. &times; 3/8" O.D. and 3/8" I.D. &times; 5/8" O.D., covering standard peristaltic pump head bore sizes. The female sealing cap included with each assembly caps the connector between uses, maintaining sterility. Overcaps: Vented and Solid Overcaps are secondary dust and contamination barriers placed directly on top of the BioFactory adaptor caps during culture incubation. The vented overcap contains a 0.22 &micro;m hydrophobic breathable membrane, allowing gas exchange through the cap stack while blocking particles and microbial contamination &mdash; used during active shaker or orbital incubation when the unit's primary cap is an adaptor cap rather than a vented cap. The solid overcap provides an additional dust and contamination seal with no gas exchange &mdash; used for short-term storage or transport outside the incubator. SPT-50 Silicone Hose SPT-50 silicone hose (3/8" I.D. &times; 5/8" O.D., 50 inches / 15 meters) is the bulk tubing used to connect multiple BioFactory units in series, connect BioFactory ports to peristaltic pump heads, or extend the reach of the transfer cap assembly. SPT-50 tubing is manufactured from silicone, is individually wrapped and sterile, and passes dissolution testing per BPOG (BioPhorum Operations Group) criteria per NEST product documentation, supporting its use in biopharmaceutical production environments. Premium Accessory Package NEST offers a BioFactory Accessory Premium Package that includes 2 different adaptor caps, 1 hose clamp, 1 adaptor connector, 1 vent filter (50 mm), 1 additional hose clamp, and 15 m of SPT-50 silicone hose &mdash; all individually wrapped and sterile in a single kit. This package provides all components needed to set up a closed-system BioFactory media management workflow from a single kit, reducing the number of individual part orders required. Vent Filters for Gas Exchange Management For BioFactory units using adaptor caps rather than standard vented caps, 50 mm inline vent filters (0.22 &micro;m hydrophobic PTFE membrane) are installed on the air inlet/outlet tubing line to maintain sterile gas exchange during media addition, harvest, and incubation. The vent filter is rated for high flow rates without creating excessive back-pressure that would resist liquid movement through the system. Compatibility: Narrow-Mouth BioFactory Units Only All BioFactory accessory caps and tubing assemblies are designed exclusively for the narrow-mouth BioFactory configuration. Wide-mouth BioFactory units use standard wide-mouth vent and plug seal caps only; they are not compatible with accessory cap assemblies. Confirm that your BioFactory units have narrow-mouth ports before ordering the accessory cap set. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST BioFactory&trade; accessory silicone tubing and cap assemblies are manufactured from USP Class VI silicone (TPE/silicone) and polycarbonate or PP cap bodies, individually wrapped and sterile. All components have passed dissolution testing per BPOG dissolution test criteria conducted by a certified third-party laboratory per NEST Scientific documentation. The standard Luer head connections conform to ISO 594 / GB/T 1962 for reliable compatibility with standard laboratory fittings. Vent filters use a 0.22 &micro;m hydrophobic PTFE membrane; overcaps use a 0.22 &micro;m hydrophobic membrane. NEST BioFactory accessories are carried by MBP alongside BioFactory units. 
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Need the right BioFactory setup? <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact MBP today</a> for technical assistance.

Biofactory System Accessories

Hose Clamp for 12-18 mm diameter hoses, each, Sterile, 1/pk, 10/cs

Sterile hose clamp (743001) for 12–18 mm diameter flexible tubing, compatible with BioFactory and bioprocess tubing assemblies. Supplied 1 per sterile pack, 10 per case — for secure, contamination-free hose sealing in closed cell culture systems.

Cell Culture Plates

<h2 class="p2"> What are Cell Culture Plates?</h2>
 Cell culture plates are sterile, single-use polystyrene multi-well vessels used to grow, treat, and analyze cells in parallel across multiple conditions in a single vessel. All formats are E-beam sterilized to SAL 10⁻⁶, certified DNase/RNase-free and non-pyrogenic, and individually wrapped in peelable film. Choose TC-treated flat-bottom plates for adherent mammalian cell culture, assays, and transfection; choose non-treated plates for suspension, hematopoietic, and hybridoma cells; choose glass-bottom plates for live-cell confocal and high-resolution fluorescence imaging.
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<h2 class="p1">What you will find:</h2>
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<li data-section-id="1rzg2e5" data-start="0" data-end="138"><a href="cell-culture-plates/non-treated-cell-culture-plates">Non-Treated Cell Culture Plates:</a> Designed for suspension cultures and non-adherent cell lines where cell attachment is not required.</li>
<li data-section-id="1f2n3q2" data-start="140" data-end="295"><a href="cell-culture-plates/treated-cell-culture-plates">Treated Cell Culture Plates:</a> Tissue-culture-treated surface promotes reliable attachment and growth of adherent cells for routine culture and assays.</li>
<li data-section-id="1dji5w9" data-start="297" data-end="442" data-is-last-node=""><a href="cell-culture-plates/glass-bottom-cell-culture-plates">Glass Bottom Cell Culture Plates:</a> Glass-bottom wells provide superior optical clarity for high-resolution microscopy and live-cell imaging.</li>
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<h2 class="p2">How to Choose Cell Culture Plates</h2>
 Well Count by Application Scale 6-well plates (9.4 cm&sup2;/well) are the standard for transfection, siRNA knockdown, Western blot sample preparation, luciferase reporter assays, and colony counting &mdash; any protocol that requires &ge;5 &times; 10⁶ cells per condition. 12-well plates (3.8 cm&sup2;/well) support medium-scale culture for ELISA lysate preparation, flow cytometry staining, and viral infection studies. 24-well plates (1.9 cm&sup2;/well) are versatile for drug dose-response at medium throughput, primary cell culture, and viral plaque assays. 48-well plates (0.95 cm&sup2;/well) bridge 24-well and 96-well scale for intermediate throughput experiments. 96-well plates (0.32 cm&sup2;/well) are the workhorse for high-throughput cell viability (MTS, MTT, CCK-8), cytotoxicity, ELISA, reporter gene, and compound screening assays. 384-well plates are used for ultra-high-throughput screening (HTS), where reagent cost per well drives miniaturization. TC-Treated vs. Non-Treated vs. Glass Bottom TC-treated (vacuum plasma&ndash;treated, contact angle &lt;10&deg;) flat-bottom plates support adherent mammalian cell monolayer formation &mdash; required for HeLa, HEK293 (adherent), CHO-K1, Vero, A549, MCF-7, and primary cells. Non-treated plates maintain a hydrophobic surface that prevents attachment &mdash; used for suspension cell lines (Jurkat, K562, HL-60), hybridoma culture, and spheroid/organoid formation in U-bottom wells. Glass-bottom plates combine a polystyrene plate body with a borosilicate coverglass floor (0.16&ndash;0.19 mm thickness), matching the No. 1.5 coverglass standard required for confocal, TIRF, and super-resolution microscopy. Flat vs. Round (U) vs. V-Bottom Well Geometry Flat-bottom wells provide a flat imaging surface for phase-contrast and fluorescence microscopy, uniform cell monolayer coverage, and reliable OD measurements in plate readers. Round (U)-bottom wells concentrate cells and particles to the well centre by gravity &mdash; used for rosette assays, hemagglutination inhibition (HAI), and hanging-drop spheroid culture. V-bottom wells have a sharp vertex that pellets cells tightly for wash steps in flow cytometry and immunophenotyping panels. Plate Lid and Evaporation Management NEST Scientific cell culture plates ship with a clear polystyrene lid. For high-throughput assays running 72&ndash;96 hours, edge-well evaporation in 96-well and 384-well formats can introduce artefacts; NEST Low Evaporation EDGE plates have an inter-wall design that reduces edge-well evaporation by maintaining humidity in the peripheral wells during long incubations. 
<h2 class="p2"> Specifications Context</h2>
 NEST Scientific cell culture plates are manufactured from 100% virgin polystyrene in ISO 8 (Class 10,000) cleanrooms. TC treatment uses vacuum plasma technology; the lot-release criterion is contact angle &lt;10&deg;. All plates conform to ANSI/SLAS 1-2004 through 4-2004 footprint and well-position standards (SBS format), ensuring compatibility with automated liquid handling platforms, plate readers, and automated incubators. E-beam sterilization to SAL 10⁻⁶. Plates are individually wrapped in peelable Tyvek-equivalent film for GMP cleanroom entry; lids are attached. Alphanumeric well markings (A&ndash;H rows, 1&ndash;12 columns for 96-well) are standard across all formats. NEST Scientific is ISO 9001:2015 and ISO 13485 certified with FDA registration. As of 2026, MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center. 
Pick your format, culture your cells, and get consistent results every time. <a href="https://mbpinc.net/contact-us">Contact MBP </a>and get a tailored quote today!

<h2 class="p2">What are TC-Treated Cell Culture Plates?</h2>
 TC-treated (tissue culture&ndash;treated) cell culture plates are sterile, single-use multi-well polystyrene plates whose well surfaces have been modified by vacuum plasma treatment to introduce negatively charged oxygen-containing groups (carboxyl, carbonyl, hydroxyl), reducing the water contact angle from ~75&ndash;90&deg; (untreated PS) to below 10&deg; (hydrophilic). This surface modification directly enables attachment, spreading, and monolayer formation of adherent mammalian cell lines. TC-treated plates are the default format for all adherent mammalian cultures, cell-based assays, ELISA preparation, compound cytotoxicity screening, transfection, and gene expression studies.&nbsp; 
<h2 class="p1">What you will find:</h2>
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&bull; 6, 12, 24, 48, and 96 well formats for different workflows &bull; Tissue-culture-treated surfaces for reliable cell attachment &bull; Clear plates for easy monitoring under the microscope &bull; Uniform wells for consistent seeding and growth &bull; Sterile options to maintain clean conditions &bull; Lids designed to reduce contamination and evaporation
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<h2 class="p2">How to Choose TC-Treated Cell Culture Plates</h2>
 Well Format by Cell Number and Assay Type 6-well TC-treated plates (9.4 cm&sup2;/well) are the standard for Western blot preparation, siRNA transfection, viral infection, protein overexpression, and colony-forming unit (CFU) assays requiring &ge;3 &times; 10⁶ cells per condition. 12-well plates (3.8 cm&sup2;/well) suit medium-scale reporter assays, luciferase quantification, and ELISA from cell lysate. 24-well plates (1.9 cm&sup2;/well) are versatile for cytotoxicity dose-response in 4&ndash;6 conditions per plate, primary cell experiments, and viral plaque assays. 96-well flat-bottom TC-treated plates (0.32 cm&sup2;/well) are the universal format for cell viability (MTS, MTT, CCK-8), compound screening, ELISA, reporter gene assays, and qPCR lysate preparation across 8&ndash;12 concentrations with triplicate wells. 384-well plates reduce reagent cost per compound for HTS drug discovery programs. Lot-to-Lot TC Treatment Consistency For longitudinal drug screening, biomarker validation, or multi-site studies, lot-to-lot variation in TC treatment can alter cell attachment density, proliferation rate, and assay Z-factor. NEST Scientific verifies vacuum plasma treatment by water contact angle goniometry on each manufacturing lot; the release criterion is contact angle &lt;10&deg;. Request lot certificates when adopting TC-treated plates for GLP- or GMP-regulated assay workflows. Low-Evaporation EDGE Plates For 96-well dose-response assays running 72&ndash;96 hours, peripheral (edge) well evaporation can increase medium osmolality in the outer ring by 10&ndash;20% versus inner wells, introducing a systematic bias in IC50 determinations. NEST Low Evaporation EDGE 96-well TC-treated plates use an internal well ridge design that reduces edge-well evaporation during extended incubations. Use EDGE plates for any dose-response study where incubation time exceeds 48 hours. Cell Line Compatibility TC-treated NEST plates support attachment and monolayer culture of: HeLa (cervical), HEK293 (adherent), A549 (lung), MCF-7 (breast), U2OS (bone), CHO-K1 (ovary, adherent), Vero (kidney), MDCK (canine kidney), NIH 3T3 (mouse fibroblast), MRC-5, 2BS, 293T, L-929, and standard primary cultures including dermal fibroblasts, endothelial cells, and epithelial cells. Do NOT use TC-treated plates for Jurkat, K562, HL-60, suspension CHO-S, hybridomas, Sf9, Hi5, or any non-adherent cell line. Plate Reader and Automation Compatibility NEST Scientific TC-treated 96-well flat plates are compatible with all common microplate readers (BioTek Synergy H1, Molecular Devices SpectraMax i3x, PerkinElmer EnVision, BMG Labtech CLARIOstar) for absorbance, fluorescence intensity, and luminescence detection. Plates conform to ANSI/SLAS 1-2004 through 4-2004 SBS dimensions for automated liquid handlers (Tecan Fluent, Hamilton STAR, Beckman Coulter Biomek i7). 
<h2 class="p2"> Specifications Context</h2>
 NEST Scientific TC-treated cell culture plates are manufactured from 100% virgin polystyrene in ISO 8 cleanrooms. Vacuum plasma TC treatment is verified by water contact angle goniometry; the lot release criterion: contact angle &lt;10&deg;. Plates conform to ANSI/SLAS 1-2004 through 4-2004 SBS footprint. E-beam sterilized to SAL 10⁻⁶; DNase/RNase-free, non-pyrogenic per lot certificate. Clear polystyrene lids included; individually wrapped in peelable film. Alphanumeric well coordinates on all &ge;12-well formats. NEST Scientific is ISO 9001:2015 and ISO 13485 certified with FDA registration. As of 2026, NEST Scientific TC-treated plates across all core well formats (6- through 384-well) are stocked by MBP. 
Use treated plates when attachment drives your results. <a href="https://mbpinc.net/contact-us">Contact the MBP team</a> today to get reliable plates for keeping your cells anchored, healthy, and consistent across every well.&nbsp;

Treated Cell Culture Plates

<h2 class="p1"> What are Non-Treated Cell Culture Plates?</h2>
 Non-treated cell culture plates are sterile, single-use multi-well polystyrene plates whose well surfaces retain the original hydrophobic, unmodified polystyrene character &mdash; no plasma, corona, or chemical treatment is applied. The hydrophobic surface (contact angle ~75&ndash;90&deg;) prevents cell attachment, making these plates the correct choice for suspension cell lines, hematopoietic cells, hybridoma culture, spheroid and organoid formation, and any experiment where surface adhesion would alter the cell phenotype or experimental readout. Non-treated plates are the correct default for all suspension-growing cell lines and for any 3D culture format &mdash; spheroids, organoids, embryoid bodies &mdash; where surface attachment is undesired.
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<h2 class="p1">What you will find:</h2>
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<ul>
<li>6, 12, 24, 48, and 96 well formats for different workflows</li>
<li>Untreated surfaces with no coating or modification</li>
<li>Clear plates for easy observation and monitoring</li>
<li>Uniform wells for consistent sample handling</li>
<li>Sterile options to maintain clean conditions</li>
<li>Lids designed to reduce contamination and evaporation</li>
</ul>
<h2 class="p1"> How to Choose Non-Treated Cell Culture Plates</h2>
 Suspension Cell Lines Requiring Non-Treated Plates Non-treated flat-bottom plates support maintenance and assays for Jurkat T-cells, K562 erythroleukemia, HL-60 promyelocytes, Raji B-cells, THP-1 monocytes, MOLM-13, and Daudi cells. Primary hematopoietic cells &mdash; PBMCs, CD34⁺ progenitors, NK cells, and bone marrow isolates &mdash; require non-treated surfaces to prevent integrin-triggered differentiation signals from TC-treated polystyrene. For hybridoma culture during HAT selection, non-treated 96-well U-bottom plates are the standard format. U-Bottom vs. Flat-Bottom for Non-Treated Plates Non-treated flat-bottom plates are the standard for suspension cell viability assays, cytotoxicity screens, and ELISA from non-adherent lysates, where wells need a uniform optical path and a flat imaging surface. Non-treated U-bottom 96-well plates concentrate non-adherent cells to the well center by gravity, producing cell pellets that are easier to aspirate during wash steps &mdash; essential for immunophenotyping panels with multiple antibody labeling and wash cycles before flow cytometry. Spheroid and 3D Culture in Non-Treated Plates Non-treated round-bottom (U-bottom) 96-well plates are widely used for hanging-drop spheroid formation and gravity-aggregated tumor spheroid culture. The hydrophobic, non-adhesive well surface combined with U-bottom geometry causes cells to aggregate at the well vertex by gravity, forming compact spheroids over 24&ndash;72 hours without scaffolding. Spheroids formed in non-treated U-bottom 96-well plates are directly measurable in the plate using a plate reader or confocal microscope with an appropriate working distance. Assay Compatibility Non-treated plates are fully compatible with cell viability reagents (CellTiter-Glo, CellTiter-Aqueous MTS, PrestoBlue), cytokine ELISA from cell supernatants, luciferase reporter assays from suspension-cell lysates, and flow cytometry sample preparation. For assays requiring a flat optical surface with minimal well-to-well distance variation, flat-bottom non-treated plates are preferred over U-bottom. Sterility and Purity Grade NEST Scientific non-treated plates are E-beam sterilized to SAL 10⁻⁶, certified DNase/RNase-free, non-pyrogenic, and individually wrapped in peelable sterile film. They are manufactured from 100% virgin polystyrene in ISO 8 cleanrooms under ISO 9001:2015 and ISO 13485 quality management. No additional surface chemistry or coating is applied; the non-treated specification is confirmed by water contact angle measurement per NEST lot release protocol. 
<h2 class="p1"> Specifications Context</h2>
 NEST Scientific non-treated plates are manufactured from 100% virgin polystyrene in ISO 8 cleanrooms; the non-treated well surface has a water contact angle of ~75&ndash;90&deg; (hydrophobic) confirmed per lot release. All plates conform to ANSI/SLAS SBS footprint standards (127.76 &times; 85.48 mm), ensuring compatibility with automated liquid handlers and plate readers. Alphanumeric well coordinates are printed on all plates &ge;12-well format. Package box double functions as a reagent reservoir for multi-channel pipette operations per NEST design. As of 2026, NEST Scientific non-treated plates in all core formats are stocked by MBP. 
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Use non-treated plates when you want full control over how your cells interact with the surface. Keep conditions simple. Keep results predictable. <a href="https://mbpinc.net/contact-us">Contact the MBP team</a> now to get a tailored quote.

Non-Treated Cell Culture Plates

DataSheet

Low Evap EDGE Plate, Flat Bottom, Sterile

714001 by NEST Scientific is a sterile flat-bottom Low Evap EDGE culture plate with edge grooves that reduce the edge effect, a low-evaporation lid, and corner feet for inverted microscope imaging. E-beam sterilized; TC-treated and non-treated surfaces available. DNase/RNase-free per lot; individually wrapped, 1/pk, 50/cs.

6 Well Low Evap EDGE Plate, Flat Bottom, Non-Treated, Sterile, 1/pk, 50/cs

96 Well Low Evap EDGE Plate, Flat Bottom, Non-Treated, Sterile, 1/pk, 50/cs

6 Well Low Evap EDGE Plate, Flat Bottom, TC, Sterile, 1/pk, 50/cs

96 Well Low Evap EDGE Plate, Flat Bottom, TC, Sterile, 1/pk, 100/cs

Multi-layer Cell Culture Flasks

<h2 dir="ltr"> What Are Multi-Layer Cell Culture Flasks?</h2>
 Multi-layer cell culture flasks are closed, TC-treated polystyrene vessels in which multiple horizontal growth chambers are stacked within a single T-175-equivalent footprint. The 3-layer format delivers 525 cm&sup2; of growth surface; the 5-layer format delivers 875 cm&sup2; &mdash; representing a 3&times; and 5&times; increase over a standard T-175 flask, respectively. They are used for adherent mammalian cell expansion workflows, including vaccine production, virus amplification, CHO-cell bioprocessing, and cell-based assay scale-up. Choose multi-layer flasks when you need &gt;T-175 yields without adding incubator shelves.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
3-Layer Cell Flasks &ndash; Increased surface area for mid-scale expansion.
</li>
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5-Layer Cell Flasks &ndash; Optimized for high-density, large-scale cell growth.
</li>
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Seal Caps &ndash; Provides a secure, liquid-tight closure for static cultures.
</li>
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Vent Caps &ndash; Features a 0.22&mu;m membrane for continuous sterile gas exchange.
</li>
</ul>
<h2 dir="ltr"> How to Choose Multi-Layer Cell Culture Flasks</h2>
 Number of Layers A 3-layer flask (525 cm&sup2;) handles moderate scale-up and is easier to prime with media; a 5-layer flask (875 cm&sup2;) maximises yield per incubator position but requires 125&ndash;200 mL of media for even distribution. Match the layer count to your target cell number per passage. Cap Type: Vent vs Plug-Seal Vent caps incorporate a 0.2 &micro;m hydrophobic PTFE membrane for passive CO₂/O₂ exchange &mdash; use when incubating in a 5% CO₂ environment. Plug-seal caps provide a hermetic closure for short-term transport or anaerobic culture. Surface Treatment Tissue culture&ndash;treated (TC-treated) surfaces are plasma-treated to a hydrophilic, negatively charged state that promotes adherent cell attachment; use untreated variants only for suspension cultures or specific coating experiments. Material &amp; Sterilisation Look for flasks made from 100% virgin, USP Class VI medical-grade polystyrene, sterilised by E-beam to SAL 10⁻⁶, and certified DNase/RNase-free and non-pyrogenic. Lot numbers printed on each flask support GMP traceability. Serological Pipette Access Flasks accepting 10 mL serological pipettes directly allow liquid aspiration and cell harvest without additional transfer steps &mdash; confirm neck design before ordering for high-throughput workflows. 
<h2 dir="ltr"> Specifications Context</h2>
 Both 3-layer and 5-layer formats share common material specs: 100% virgin polystyrene body, HDPE cap, and PTFE vent membrane (vent cap models). Sterilisation to SAL 10⁻⁶ via E-beam irradiation (ISO 11137) is standard. As of 2026, the Nest Scientific 3-layer (cat. 731302) and 5-layer (cat. 731001/731002) remain the most widely distributed mid-tier options. For GMP applications requiring cGMP compliance documentation, Falcon/Corning multi-flasks are the established reference; MBP's Nest Scientific line suits research and process development. 
Achieve superior cell growth with validated multi-layer systems. <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact the experts at Molecular Biology Products Inc.</a> for a quote to optimize your laboratory&rsquo;s output and reliability

Multi-layer Cell Culture Flask Specifications

Multi-layer Cell Culture Flask

731001 by NEST Scientific — 5-layer multi-layer cell culture flask with 870 cm² TC-treated growth area. Vent cap, RNase/DNase-free, sterile, 1/pk. For large-scale adherent cell expansion and protein or viral vector production.

5-Layer Cell Culture Flask, Plug Seal Cap, Straight Neck, TC, sterile 1/pk, 8/cs

3-Layer Cell Culture Flask, Plug Seal Cap, Straight Neck, TC, sterile 1/pk, 12/cs

5-Layer Cell Culture Flask, Vent Cap, Straight Neck, TC, sterile 1/pk, 8/cs

3-Layer Cell Culture Flask, Vent Cap, Straight Neck, TC, sterile 1/pk, 12/cs

Chamber Slides

<h2 dir="ltr"> What are Cell Culture Chamber Slides?</h2>
 Cell culture chamber slides are sterile, single-use systems combining a standard microscope slide (25 mm &times; 75 mm borosilicate glass, 1.0 &plusmn; 0.1 mm thickness) with 2, 4, or 8 removable polystyrene or PS chamber walls mounted directly on the glass surface, allowing cells to be grown, treated, fixed, and immunostained in situ on the microscope slide, without any cell transfer step between culture and imaging. After culture and staining, the chamber walls are removed using an easy-open flip release mechanism, leaving cells attached to the glass slide for coverslip mounting and brightfield, widefield fluorescence, or confocal microscopy. Chamber slides are used for immunocytochemistry (ICC), viral studies, toxicity testing, in situ hybridization (FISH), chromosomal studies, and any application requiring high-quality microscopy of cells grown directly on a glass surface.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Ultra-thin borosilicate glass and USP Class VI polymers minimize auto-fluorescence for high-resolution microscopy.
</li>
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Precision-molded gaskets provide hermetic, cross-contamination-free separation between experimental wells.
</li>
<li dir="ltr" aria-level="1">
Optimize reagent use with versatile 1, 2, 4, and 8-well configurations for high-throughput cell culture.
</li>
<li dir="ltr" aria-level="1">
Certified RNase-free, DNase-free, and non-pyrogenic to ensure the reliability of sensitive molecular assays.
</li>
</ul>
&nbsp;
<h2 dir="ltr">How to Choose Chamber Slides</h2>
 Well Count by Application Scale and Cell Number 2-well chamber slides (growth area 4.55 cm&sup2;/well, working volume 1.20&ndash;2.50 mL) provide the largest growth area per well &mdash; suited for applications requiring large cell numbers per condition (western blot input from chamber-grown cells, cytokine stimulation with media harvest, viral infection with high MOI). 4-well slides (approximately 2.1 cm&sup2;/well, 0.5&ndash;1.0 mL) are the intermediate format for 2&ndash;4 parallel treatment conditions on a single slide. 8-well slides (0.98 cm&sup2;/well, 0.20&ndash;0.60 mL) allow up to 8 conditions per slide &mdash; the standard for immunofluorescence panels with multiple treatment conditions, antibody titrations, and cell line comparison arrays on a single glass slide. Clear vs. White vs. Black Chamber Walls Clear polystyrene chamber walls provide visual inspection of the culture through the side wall, allowing phase-contrast observation at low magnification during culture without removing the lid. White chamber walls provide maximum contrast between the well walls and the cell monolayer during visual inspection &mdash; useful for monitoring culture progress in labs without a microscope at the culture bench. Black chamber walls (available in 8-well format, NEST catalog 230118) reduce optical cross-talk between adjacent wells during fluorescence imaging with a scanning stage or microplate fluorescence reader. Glass vs. Plastic Substrate NEST Scientific chamber slides are built on borosilicate glass slides (1.0 &plusmn; 0.1 mm thickness), which are thicker than No. 1.5 coverglass (0.17 mm) &mdash; standard for brightfield, phase-contrast, and widefield fluorescence microscopy with long-working-distance 10&times;, 20&times;, and 40&times; dry objectives. For oil-immersion 60&times; or 100&times; objectives corrected for No. 1.5 coverglass, mount a No. 1.5 coverslip (0.17 mm) over the cells after staining and use the coverslip as the imaging window. Some chamber slide manufacturers use thinner glass (No. 1.5); confirm the glass thickness against your objective's specification. Chamber Wall Removal NEST Scientific chamber slides use an easy-open flip mechanism &mdash; a tab or lever on the chamber wall structure that releases the chamber body from the glass slide without mechanical blade or knife use. After cell fixation, staining, and washing in the assembled slide, the chambers are removed by lifting the release tab, leaving cells adhered to the glass substrate. Wash and mount a coverslip directly for widefield or confocal imaging. Surface Treatment The NEST Scientific borosilicate glass substrate is described as naturally charged &mdash; providing a good growth surface for adherent cells without applied polymer coatings. For primary neurons, primary endothelial cells, or other cells requiring enhanced attachment, pre-coat the glass surface with poly-D-lysine, laminin, fibronectin, or Matrigel before seeding. No surface treatment is applied to the glass by NEST Scientific as the default. 
<h2 dir="ltr"> Specifications Context</h2>
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NEST Scientific cell culture chamber slides are manufactured with borosilicate glass slides (25 &times; 75 mm, 1.0 &plusmn; 0.1 mm thickness) and polystyrene chamber walls. Chamber walls are non-cytotoxic, non-pyrogenic, and DNase/RNase/human DNA-free per lot certificate. No chemical adhesives are used in the chamber-to-glass bond. Chamber walls are released via an easy-open flip mechanism without tools. NEST Scientific is ISO 9001:2015 and ISO 13485 certified with FDA registration. Each pack contains 6 slides; 12 slides per case (2 packs &times; 6). As of 2026, NEST Scientific chamber slides in 2-, 4-, and 8-well formats with clear, white, and black walls are stocked by MBP.
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Achieve consistent, reproducible imaging results with reliable labware. The <a href="https://mbpinc.net/contact-us" title="MBP team" style="color: #236fa1;">MBP team</a> can help evaluate your current setup and recommend solutions tailored to your microscopy workflow.&nbsp;

NEST Scientific Cell Culture Chamber Slide

Nest Cell Culture Chamber Slides, with glass slide

230101 by NEST Scientific — 1-well polystyrene chamber slide on a borosilicate glass base. 9.40 cm² growth area, 2.5–5.5 mL working volume, TC-treated, sterile, 6/pk. Designed for immunofluorescence and high-resolution microscopy workflows.

Nest Cell Culture Chamber Slides, 1 well w/glass slide, Clear, 9.40 cm2, 2.5-5.5ml, 6/pk, 12/cs

Nest Cell Culture Chamber Slides, 2 well w/glass slide, Clear, 4.55 cm2, 1.2-2.5ml, 6/pk, 12/cs

Nest Cell Culture Chamber Slides, 4 well w/glass slide, Clear, 2.13 cm2, 0.5-1.3ml, 6/pk, 12/cs

Nest Cell Culture Chamber Slides, 8 well w/glass slide, Clear, 0.98 cm2, 0.2-0.6ml, 6/pk, 12/cs

Nest Cell Culture Chamber Slides, 1 well w/glass slide, White, 9.40 cm2, 2.5-5.5ml, 6/pk, 12/cs

Nest Cell Culture Chamber Slides, 2 well w/glass slide, White, 4.55 cm2, 1.2-2.5ml, 6/pk, 12/cs

Nest Cell Culture Chamber Slides, 4 well w/glass slide, White, 2.13 cm2, 0.5-1.3ml, 6/pk, 12/cs

Nest Cell Culture Chamber Slides, 8 well w/glass slide, White, 0.98 cm2, 0.2-0.6ml, 6/pk, 12/cs

Nest Cell Culture Chamber Slides, 1 well w/glass slide, Black, 9.40 cm2, 2.5-5.5ml, 6/pk, 12/cs

Nest Cell Culture Chamber Slides, 2 well w/glass slide, Black, 4.55 cm2, 1.2-2.5ml, 6/pk, 12/cs

Nest Cell Culture Chamber Slides, 4 well w/glass slide, Black, 2.13 cm2, 0.5-1.3ml, 6/pk, 12/cs

Nest Cell Culture Chamber Slides, 8 well w/glass slide, Black, 0.98 cm2, 0.2-0.6ml, 6/pk, 12/cs

Cell Strainers

<h2 class="p1"> What are Cell Strainers?</h2>
 Cell strainers are sterile, single-use nylon mesh filter caps that sit in the opening of a 50 mL conical centrifuge tube, used to disaggregate tissue homogenates, tumor biopsies, lymph nodes, spleens, and bone marrow aspirates into uniform single-cell suspensions for downstream applications including flow cytometry, primary cell culture, FACS sorting, and immunophenotyping. The strainer is placed filter-side down in the collection tube, the tissue suspension is added to the strainer basket and pushed through the nylon mesh using the plunger of a syringe, the wet end of a syringe barrel, or the back of a sterile 1 mL syringe. Choose 40 &micro;m for fine single-cell suspension preparation from soft tissues; 70 &micro;m for standard tissue disaggregation (the most commonly used pore size); 100 &micro;m for pre-filtration of coarse tissue homogenates and large spheroids.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
40&mu;m Cell Strainers for fine-scale molecular filtration
</li>
<li dir="ltr" aria-level="1">
70&mu;m Cell Strainers for routine primary cell isolation
</li>
<li dir="ltr" aria-level="1">
100&mu;m Cell Strainers for large cluster separation
</li>
<li dir="ltr" aria-level="1">
Sterile Nylon Mesh Strainers for precise pore consistency
</li>
<li dir="ltr" aria-level="1">
Color-Coded Strainers for easy micron-size identification
</li>
<li dir="ltr" aria-level="1">
Universal Fit Strainers for standard 50mL centrifuge tubes
</li>
</ul>
<h2 class="p1"> How to Choose Cell Strainers</h2>
 Pore Size by Application 40 &micro;m strainers (blue) produce the finest filtrate, retaining cell aggregates and larger tissue debris while passing single cells and small cell clusters. They are the standard for preparing highly uniform single-cell suspensions from soft tissues (spleen, thymus, lymph nodes) for flow cytometry, where doublets must be minimized. 40 &micro;m strainers have lower flow rates and clog more readily with dense or fibrous tissues. 70 &micro;m strainers (white) are the most commonly used pore size in cell biology &mdash; they balance efficient single-cell passage with adequate retention of fibrous connective tissue and undigested tissue chunks. Standard for tumor dissociation, liver perfusion single-cell prep, and any tissue where a moderate doublet rate is acceptable. 100 &micro;m strainers (yellow) pass cell clusters and small organoids while retaining large undissociated tissue pieces; used as a first-pass pre-filtration step before 70 &micro;m or 40 &micro;m filtration of tough fibrous tissues, and for filtering 3D tumor spheroids grown in 96-well U-bottom plates before reseeding. Color-Coding for Laboratory Workflow NEST Scientific cell strainers follow a universal color-coding convention: 40 &micro;m = blue, 70 &micro;m = white, 100 &micro;m = yellow. This matches the color convention used by BD Biosciences Falcon cell strainers, allowing unambiguous pore-size identification during multi-step tissue disaggregation protocols without reading the label at every step. 50 mL Tube Compatibility NEST Scientific cell strainers are designed with a flange diameter that fits securely into the opening of 50 mL conical centrifuge tubes from most major brands, including BD Falcon, TPP, and MidSci, per NEST Scientific product documentation. A molded tab on the upper ring facilitates sterile handling during tube loading without touching the mesh surface. Tissue Types and Recommended Pore Size For spleen, thymus, and lymph nodes: 40 &micro;m or 70 &micro;m. For bone marrow: 70 &micro;m. For tumor biopsy (mechanically or enzymatically dissociated): 40 &micro;m (after enzyme digestion) or 70 &micro;m (mechanical disaggregation only). For liver (perfusion-dissociated hepatocytes): 70 &micro;m or 100 &micro;m. For 3D spheroid disaggregation: 40 &micro;m or 70 &micro;m, depending on the desired cluster size in the filtrate. For flow cytometry sorting (minimize doublets): 40 &micro;m immediately before loading the sorter. 
<h2 class="p1"> Compare NEST Scientific Cell Strainer SKUs </h2>
<ul>
<li data-section-id="16rq5ic" data-start="0" data-end="126">40 &micro;m (Blue): NEST Catalog 258369; 50/case; suitable for fine single-cell suspension and flow cytometry pre-filtration.</li>
<li data-section-id="10iabbd" data-start="128" data-end="251">70 &micro;m (White): NEST Catalog 258368; 50/case; suitable for standard tissue disaggregation and universal applications.</li>
<li data-section-id="toszed" data-start="253" data-end="390" data-is-last-node="">100 &micro;m (Yellow): NEST Catalog 258367; 50/case; suitable for pre-filtration, coarse tissue processing, and spheroid size selection.</li>
</ul>
<h2 class="p1"> Specifications Context</h2>
 NEST Scientific cell strainers are manufactured from nylon mesh bonded to a polypropylene (PP) frame with a tab-molded upper ring for sterile handling. Pore sizes are 40 &micro;m, 70 &micro;m, and 100 &micro;m; mesh pore spacing is uniform per manufacturing lot. Compatible with 50 mL conical centrifuge tubes from BD Falcon, TPP, and MidSci per NEST Scientific USA documentation. Each strainer is sterile (gamma-irradiated), individually wrapped, non-pyrogenic, and DNase/RNase-free per lot certificate. Non-cytotoxic nylon mesh. All three pore sizes (40, 70, and 100 &micro;m) are stocked by MBP in cases of 50. 
&nbsp;
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Datsheet

Nest Scientific Cell Strainers Specifications

Nest Scientific Cell Strainers

NEST Scientific 258369 is a sterile 40 µm universal cell strainer — nylon mesh, polypropylene blue-coded frame, fits standard 50 mL conical tubes without adapters. Individually wrapped, 50 per case. For single-cell suspension preparation before flow cytometry, FACS sorting, and cell counting.

40uM Cell Strainer, universal, individually wrapped, sterile, 50/case

70uM Cell Strainer, universal, individually wrapped, sterile, 50/case

100uM Cell Strainer, universal, individually wrapped, sterile, 50/case

Media Storage Bottles

<h2 dir="ltr">What are Media Storage Bottles?</h2>
 Media storage bottles for cell culture are sterile, single-use or multi-use plastic containers used to store and dispense tissue culture media, sera, buffers, and other aqueous solutions in research and biopharmaceutical laboratory settings. Choose PC bottles for autoclavable reuse; PET for single-use media storage and transport; PETG for low-temperature archival, closed-system transfer, and pH-sensitive long-term storage.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
<a href="media-storage-bottles/petg-square-bottles">PETG Square Bottles </a>for space-saving laboratory storage
</li>
<li dir="ltr" aria-level="1">
<a href="consumables/cell-culture/media-storage-bottles/petg-bottle-with-accessories" style="color: #236fa1;">PETG Bottle With</a><a href="media-storage-bottles/petg-bottle-with-accessories" style="color: #236fa1;"> Accessories&nbsp;</a><a href="https://mbpinc.net/petg-bottle-with-accessories" title="PETG Bottle With Accessories" style="color: #236fa1; text-decoration: underline;"></a>for specialized fluid handling
</li>
<li dir="ltr" aria-level="1">
<a href="media-storage-bottles/pc-media-bottles">PET Media Bottles </a>for high-clarity reagent containment
</li>
<li dir="ltr" aria-level="1">
<a href="media-storage-bottles/pc-media-bottles">PC Media Bottles </a>for superior mechanical durability
</li>
</ul>
<h2 dir="ltr"> How to Choose Media Storage Bottles</h2>
 Material: PC vs. PET vs. PETG PC (polycarbonate) bottles are window-clear, shatter-proof, and rated for repeated autoclaving (up to 5&ndash;10 cycles) &mdash; the standard for labs that prepare and sterilize media in-house. PC is resistant to alcohols, weak acids, and aliphatic hydrocarbons; it is not compatible with strong bases or organic solvents. PC contains BPA (bisphenol A) by its monomer chemistry and is not recommended for long-term hormone-sensitive cell culture applications. PET bottles are optically clear, lighter than glass, non-cytotoxic, and non-autoclavable, rated from &minus;60 &deg;C to +60 &deg;C. They are the cost-effective standard for single-use media and sera storage in non-autoclave workflows. PETG (glycol-modified PET) adds a glycol co-monomer that reduces crystallinity, improving impact resistance, low-temperature stability to &minus;80 &deg;C, and flexibility. PETG is the preferred material for long-term storage of pH-sensitive liquids, protein/vaccine intermediates, and applications requiring closed-system accessory assemblies. Round vs. Square Bottles Round bottles (Boston round, GL45 thread) are compatible with standard bottle-top vacuum filtration systems (Millipore Stericup, Corning Costar) and are the format of choice for media vacuum-filtered in-house. Square bottles conserve refrigerator and cold room shelf space due to their flat sides and stackable design, reducing storage footprint by approximately 25% versus equivalent-volume round bottles. NEST Scientific PET and PETG square media bottles feature molded graduations and a white labeling area on two sides. Volume Range Standard media bottle volumes are 60 mL, 125 mL, 250 mL, 500 mL, and 1,000 mL, corresponding to common media and sera kit sizes. A 250 mL bottle accommodates a standard 250 mL media aliquot; a 500 mL bottle holds standard FBS lots or 500 mL media packs. Confirm that the bottle's nominal volume includes 15% additional headspace for mixing, thawing expansion, and transfer operations. Accessory Assembly vs. Standard Cap Standard PETG square media bottles ship with an HDPE screw cap &mdash; suitable for storage and manual transfer. PETG bottles with accessory caps (bi-directional transfer cap + TPE tubing + Luer lock or CPC connector, NEST catalog 3525xx series) create a fully closed aseptic liquid transfer system &mdash; used in cGMP environments, bioprocess research, and any workflow where open-pour media transfer creates contamination or regulatory risk. Sterility and Certifications For cell culture use, all media storage bottles must be certified DNase/RNase-free, non-pyrogenic (endotoxin-free), and non-cytotoxic. NEST Scientific PET and PETG media bottles meet USP Class VI and ISO 10993 biocompatibility requirements, are manufactured in ISO 8 cleanrooms, and are gamma or E-beam-sterilized. PC bottles from autoclave-capable suppliers use virgin Lexan&reg; PC resin compliant with USP Class VI.
&nbsp;
<h2 dir="ltr">Specifications Context</h2>
 NEST Scientific square PET media bottles (catalog 334xxx) and PETG media bottles (catalog 352xxx) are manufactured from 100% virgin material in ISO 8 cleanrooms. Both materials meet USP Class VI and ISO 10993 biocompatibility requirements. Bottles are gamma irradiated in the USA or E-beam sterilized to SAL 10⁻⁶; each lot is certified DNase/RNase-free, pyrogen-free, and endotoxin-free. PETG bottles feature molded graduations (not printed), smooth inner surface to minimize residue on the wall, a one-shot-molded cap seat (no inner gasket required), and a double-bagged sterile packaging format meeting GMP purification workshop material-entry requirements. NEST Scientific PET and PETG media bottles are stocked by MBP in sizes from 60 mL to 1,000 mL. 
Don't wait for a contamination crisis to upgrade your storage. <a href="https://mbpinc.net/contact-us" title="Reach out to the Molecular Biology Products Inc.">Reach out to the Molecular Biology Products Inc.</a> experts for a quote and keep your reagents locked down.

<h2>What are PC Media Bottles?</h2>
 PC (polycarbonate) media storage bottles are reusable, autoclavable, highly transparent plastic containers used to prepare, sterilize, store, and dispense tissue culture media, sera, buffers, and other aqueous solutions. Polycarbonate &mdash; a rigid aromatic polyester with carbonate linkages &mdash; is the strongest standard thermoplastic, rated for use from &minus;135 &deg;C to +135 &deg;C, making it suitable for ultra-low freezer storage through repeated autoclave sterilization at 121 &deg;C. PC is window-clear, virtually shatterproof compared to glass, and dimensionally stable after autoclaving when not placed under vacuum stress. PC media bottles are the correct choice for any in-house media or buffer preparation workflow requiring autoclave sterilization, where cost amortization across multiple autoclave cycles justifies the higher per-bottle price versus single-use PET/PETG.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
30mL PC Media Bottles for small-scale reagent sampling
</li>
<li dir="ltr" aria-level="1">
60mL PC Media Bottles for compact laboratory storage
</li>
<li dir="ltr" aria-level="1">
125mL Polycarbonate Bottles for routine buffer handling
</li>
<li dir="ltr" aria-level="1">
250mL PC Media Bottles for mid-range media volumes
</li>
<li dir="ltr" aria-level="1">
500mL PC Media Bottles for standard cell culture prep
</li>
<li dir="ltr" aria-level="1">
1000mL PC Media Bottles for large-batch reagent storage
</li>
<li dir="ltr" aria-level="1">
2000mL Large Capacity Bottles for high-volume liquid transfer
</li>
<li dir="ltr" aria-level="1">
5000mL PC Media Bottles for industrial-scale stock solutions
</li>
</ul>
<h2> How to Choose PC Media Bottles</h2>
 Number of Autoclave Cycles Standard PC lab bottles withstand 5&ndash;10 autoclave cycles at 121 &deg;C before mechanical strength begins to degrade, and discoloration may appear. Nalgene polycarbonate bottles (USP Class VI virgin resin, Thermo Fisher) are rated for use up to the manufacturer's cycle guidance. Each autoclave cycle causes mild hydrolytic attack on carbonate linkages &mdash; do not use PC bottles for vacuum applications after they have been autoclaved, as mechanical strength reduction raises implosion risk. BPA Consideration Polycarbonate contains bisphenol A (BPA) as a consequence of its carbonate-monomer chemistry. Trace BPA can leach from PC surfaces into aqueous solutions, particularly at elevated temperatures (autoclaving, warm media). For applications using estrogen receptor&ndash;positive cell lines (MCF-7, T47D), primary endocrine cells, or embryonic stem cells, BPA-free alternatives (PET or PETG) are the safer choice. For standard media preparation and non-hormone-sensitive applications, the trace BPA levels in USP Class VI PC do not affect results. Chemical Compatibility PC resists weak acids, alcohols (ethanol up to 70%), and aliphatic hydrocarbons. PC is not compatible with strong bases (NaOH, KOH), organic solvents (acetone, DMSO at high concentration), concentrated acids, or hydrolytic conditions at elevated pH &mdash; all rapidly degrade carbonate linkages, causing crazing, cracking, or failure. Never autoclave PC with alkaline or oxidizing cleaning agents. For compatibility questions, consult the manufacturer's chemical resistance guide. Round (GL45) vs. Square Format PC round bottles (Boston round profile, GL45 threaded neck) are the dominant format for media preparation because they directly accept bottle-top vacuum filtration assemblies (Millipore Stericup, Corning) and are compatible with GL45 lids and pouring rings. PC square bottles conserve refrigerator shelf space and are preferred for cold-storage media archiving. Confirm whether your bottle-top filter threading matches GL45, 38-430, or 45-400 before ordering round bottles. Volume and Headspace PC media bottles are available from 125 mL to 10 L. Standard cell culture volumes &mdash; 125 mL, 250 mL, 500 mL, 1,000 mL &mdash; correspond to common media kit sizes. Each bottle nominally includes 15% additional headspace for autoclaving thermal expansion (liquid expands ~4% on autoclave heating) and safe pouring; never fill above the marked maximum fill line before autoclaving. 
<h2> Specifications Context</h2>
 Nalgene polycarbonate bottles are manufactured from USP Class VI laboratory-grade PC resin compliant with USP and ASTM biocompatibility testing, EP monographs, EU food-contact directives, CONEG, and RoHS requirements. Temperature use range: &minus;135 &deg;C to +135 &deg;C; autoclavable at 121 &deg;C per cycle guidance. Mechanical properties: PC has a tensile strength of approximately 55&ndash;75 MPa and impact resistance that remains &gt;50 J/m at &minus;40 &deg;C, making it effectively shatterproof under typical lab handling. After autoclaving, PC retains clarity but loses some tensile strength with each cycle &mdash; monitor for crazing, discoloration, or cracks that indicate end-of-service-life. As of 2026, MBP carries PC media bottles suitable for autoclave-based media preparation workflows.
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<a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">Reach out to the Molecular Biology Products Inc.</a> experts for a quote and upgrade your storage standards today.

PC Media Bottles

Nest Scientific PC Square Media Bottles, Sterile

354314 by NEST Scientific is a sterile polycarbonate (PC) square media bottle, enzyme-free and double-bagged, for cell culture, molecular biology, genomics, and proteomics. Autoclavable at 121 °C; manufactured under ISO 13485:2016 and ISO 9001. Multiple volumes available.

30mL PC Square Media Bottles, Sterile, double bagged small pacakging, 5/pk, 40/cs

60mL PC Square Media Bottles, Sterile, double bagged small pacakging, 6/pk, 48/cs

125mL PC Square Media Bottles, Sterile, double bagged small pacakging, 6/pk, 48/cs

250mL PC Square Media Bottles, Sterile, double bagged small pacakging, 6/pk, 48/cs

500mL PC Square Media Bottles, Sterile, double bagged small pacakging, 8/pk, 24/cs

1000mL PC Square Media Bottles, Sterile, double bagged small pacakging, 4/pk, 12/cs

5L PC Square Storage Bottles, Sterile, double bagged small pacakging, 1/pk, 6/cs

Nest Scientific PC Square Media Bottles, Sterile, Tray-Packed

Nest Scientific 354313 — 30 mL sterile polycarbonate (PC) square media bottle, E-beam sterilized (SAL 10⁻⁶), DNase/RNase-free, USP Class VI, -80°C to 121°C, tray-packed, 280 per case.

30mL PC Square Media Bottles, Sterile, Tray-Packed, 40/pk, 280/cs

60mL PC Square Media Bottles, Sterile, Tray-Packed, 40/pk, 200/cs

125mL PC Square Media Bottles, Sterile, Tray-Packed, 24/pk, 96/cs

250mL PC Square Media Bottles, Sterile, Tray-Packed, 30/pk, 60/cs

500mL PC Square Storage Bottles, Sterile, Tray-Packed, 20/pk, 40/cs

1000mL PC Square Storage Bottles, Sterile, Tray-Packed, 12/pk, 24/cs

2 L PC Square Storage Bottle, Sterile, 6/pk, 12/cs

<h2 dir="ltr">What are PET Media Bottles?</h2>
 PET (polyethylene terephthalate) media storage bottles are single-use, sterile, optically clear plastic containers for storing tissue culture media, fetal bovine serum, PBS, HBSS, trypsin-EDTA, and other aqueous laboratory solutions. PET is manufactured by an injection stretch-blow molding (ISBM) process that produces glass-like optical clarity, low gas permeability (especially to CO₂ and O₂ compared to polystyrene), excellent mechanical strength, and a smooth inner surface that minimizes liquid residue. PET bottles are BPA-free, non-autoclavable, and rated for use from &minus;60 &deg;C to +60 &deg;C.
&nbsp;They are the dominant single-use format for media and sera storage in cell culture because of their glass-comparable clarity, low extractable profile, and cost-effectiveness compared to borosilicate glass. Choose PET media bottles as the default single-use format for ready-to-use media and sera storage, cold room archiving, and any application where autoclaving is not required.
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<h2 dir="ltr">What you will find:</h2>
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60mL PET Media Bottles for small-scale sampling
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125mL Sterile PET Bottles for routine reagent handling
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250mL PET Storage Bottles for mid-range media volumes
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500mL/0.5L PET Bottles for standard laboratory preparations
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1000mL/1L PET Media Bottles for large-batch stock solutions
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2L High-Volume PET Bottles for industrial-scale storage
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5L PET Media Vessels for bulk liquid containment
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Double-layer Packaged Bottles for enhanced cleanroom safety
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Square Shoulder Tray-Packed Bottles for secure, organized transport
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<h2 dir="ltr"> How to Choose PET Media Bottles</h2>
 Round vs. Square Format Round PET media bottles (Boston round, GL45) are directly compatible with bottle-top vacuum filtration systems and standard lab bottle accessories. Square PET media bottles (NEST Scientific catalog 334xxx) feature flat sides that stack efficiently in refrigerators and cold rooms, reducing footprint by approximately 25% versus round bottles and preventing rolling when stored horizontally. NEST PET square bottles have molded graduations (not printed), a white labeling area on two sides, and embossed markings for durable identification. Volume Selection PET media bottles are available in 30 mL, 60 mL, 125 mL, 250 mL, 500 mL, and 1,000 mL volumes. A 500 mL bottle accommodates a standard 500 mL aliquot of cell culture media (DMEM, RPMI, MEM) or a standard FBS lot aliquot. The 1,000 mL bottle holds a full 1 L media preparation. Each bottle includes approximately 15% additional headspace above the nominal volume for thawing expansion (&minus;20 &deg;C frozen FBS thaw) and transfer convenience. Cap Material NEST Scientific PET media bottle caps are made from HDPE (high-density polyethylene), free of heavy metals per regulatory testing. The cap is designed through a one-shot mold process without an inner gasket, fitting securely onto the bottle neck with enhanced sealing performance &mdash; an important feature for leak-proof horizontal storage and air transport. Gas Barrier Properties PET and PETG both reduce gas permeability to CO₂ and O₂ compared to polystyrene, maintaining medium pH and preventing dissolved gas equilibration during storage. For pH-sensitive media containing bicarbonate buffers, PET and PETG are materially superior to polystyrene bottles, which allow CO₂ outgassing and pH shift during storage at room temperature or 4 &deg;C. Sterility and Lot Traceability NEST Scientific PET media bottles are gamma irradiated or E-beam sterilized; each lot is certified DNase/RNase-free, human DNA-free, pyrogen-free, and non-cytotoxic per lot certificate. Lot numbers are printed on each bottle and packaging for traceability. Shelf life is 3 years from the production month per NEST lot release data. PET bottles meet FDA requirements for materials used in contact with sterile liquid pharmaceuticals. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific PET square media bottles are manufactured from 100% virgin PET resin via the ISBM process in ISO 8 cleanrooms. Material meets USP Class VI and ISO 10993 biocompatibility, FDA requirements, and USP &lt;661&gt; container testing for plastics in contact with sterile solutions. Operating temperature: &minus;60 &deg;C to +60 &deg;C; not autoclavable. Smooth inner wall minimizes residue; molded graduations on two sides. The HDPE cap is heavy-metal-free. Packaging is double-bagged in sterile format for GMP-compatible cleanroom entry. Shelf life: 3 years from production month. As of 2026, NEST Scientific PET media bottles are stocked by MBP in the full size range. 
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Don&rsquo;t wait for a compromised sample to upgrade your containment. <a href="https://mbpinc.net/contact-us">Contact now </a>and request a quote from Molecular Biology Products Inc. and keep your lab results (and your PI) stress-free.

PET Media Bottles

Nest Scientific PET Square Storage Bottles, Sterile

333004 by NEST Scientific is a sterile, double-bagged PET square storage bottle with PP cap, certified DNase-free, RNase-free, and non-pyrogenic. The square shoulder profile maximises cold storage density; suitable for media, buffers, and bioprocess intermediates. Multiple volumes available.