Cell Culture

Cell Culture Inserts

<h2 dir="ltr">What are Cell Culture Inserts?</h2>
 Cell culture inserts (also called Transwell inserts or permeable supports) are sterile, single-use polystyrene well inserts with a permeable membrane floor that suspend cells above the bottom of a standard multi-well plate, creating separate apical (upper, inside insert) and basolateral (lower, outside insert in the well below) compartments. The membrane allows diffusion of media, nutrients, metabolites, and signaling molecules between the two compartments while physically separating the two cell populations.&nbsp;Choose PET membranes for imaging and clear-bottom visualization; choose PC membranes for superior cell attachment; choose pore size based on whether cells need to migrate through the membrane (&ge;3 &micro;m) or be retained on it (0.4 &micro;m and 1 &micro;m).
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="color: #000000;" role="presentation">6 Inserts / 6-Well Plate for Tissue Engineering - Ideal for skin models and complex cell differentiation where a larger surface area is required.</li>
<li dir="ltr" style="color: #000000;" role="presentation">12 Inserts / 12-Well Plate for Transport Assays - Balanced format for nutrient diffusion studies and metabolic monitoring.</li>
<li dir="ltr" style="color: #000000;" role="presentation">12 Inserts / 24-Well Plate for&nbsp;High Throughput Screening - Designed for migration and chemotaxis assays with efficient reagent use.</li>
<li style="color: #000000;">SPLInsert&trade; Hanging formats with transparent, sterile PC or PET membranes.</li>
<li style="color: #000000;">SPLInsert&trade; Standing format for cell culture insert applications.</li>
<li style="color: #000000;">100 mm cell culture insert dishes with 3.0 &mu;m PC membranes, translucent construction, tissue-culture treatment, and sterile packaging.</li>
</ul>
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<h2 dir="ltr">How to Choose Cell Culture Inserts</h2>
 PET vs. PC Membrane PET (polyethylene terephthalate) membranes are optically transparent, allowing direct visualization of cells growing on the membrane by standard phase-contrast and fluorescence microscopy without removing the insert. PET is the first choice when imaging cells on the membrane is part of the experimental readout (tight-junction immunofluorescence, GFP localization, wound-healing on the membrane). PC (polycarbonate) membranes provide superior cell attachment due to their higher surface energy &mdash; often used for primary epithelial, endothelial, and intestinal cells that are difficult to culture on PET. PC membranes are opaque, blocking transmitted light microscopy through the membrane. Pore Size by Application 0.4 &micro;m pore inserts prevent cell migration and cell-cell contact between apical and basolateral compartments while allowing free diffusion of molecules &lt;0.4 &micro;m (nutrients, cytokines, small molecules, metabolites). Standard for epithelial barrier function assays (TEER measurement, permeability assays), secretion studies (measuring cytokine secretion from the basolateral side), and any co-culture where cell-cell contact must be prevented. 1 &micro;m pore inserts similarly prevent cell migration but allow slightly larger molecule diffusion &mdash; used for exosome transport studies. 3 &micro;m pore inserts allow poorly migratory cells (some endothelial, fibroblast lines) to migrate through. 8 &micro;m pore inserts support tumor cell migration, invasion, chemotaxis, and hematotaxis assays &mdash; the standard pore size for Boyden chamber&ndash;style migration and invasion assays. Plate Format and Insert Configuration NEST Scientific inserts are pre-packed in multi-well plates: 6-well plate format (6 inserts per plate), 12-well plate format (12 inserts per plate), 24-well plate format (12 inserts per plate). Inserts are centered in wells by their flange geometry, preventing the membrane from touching the well sides and creating a wicking path. The apical-basolateral gap (distance from membrane floor to well bottom) is designed to allow media access to the basolateral face and pipette access to the lower chamber for sampling. TC-Treated vs. Non-Treated Membrane TC-treated membrane inserts promote adherent mammalian cell monolayer formation on the insert membrane. Non-treated membrane inserts (some PC formats) are used for migration and invasion assays where cells in the apical chamber must actively migrate through the membrane pore to reach the basolateral side &mdash; a non-treated surface reduces passive cell adhesion that would produce false-positive migration signals. Transferability NEST Scientific cell culture inserts can be transferred from one plate to a fresh plate for media changes, cumulative secretion studies, and time-course experiments &mdash; a key advantage over the non-insert well format. Transfer the insert using sterile forceps or the insert flange. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific cell culture inserts are manufactured from polystyrene with PET or PC membranes in TC-treated (vacuum plasma, contact angle &lt;10&deg;) or non-treated formats. Inserts are packed in individually sealed, peelable Tyvek film and blister packaging for sterility assurance &mdash; one plate per pack, sterile. E-beam sterilized to SAL 10⁻⁶; DNase/RNase-free and non-pyrogenic per lot. The insert flange geometry is designed to center the insert in the well, preventing membrane-wall contact and wicking. NEST Scientific cell culture inserts in PET and PC membranes across the four pore sizes (0.4, 1, 3, 8 &micro;m) and three plate formats (6-, 12-, 24-well) are available from MBP. 
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Bridge the gap between routine culture and physiological discovery. Optimize your laboratory procurement with&nbsp;Molecular Biology Products Inc. <a href="https://mbpinc.net/contact-us">Connect with our specialists</a> for a customized quote today.

Datsheet

DataSheet

Cell Culture Insert Specifications

100mm Cell Culture Insert-Dish, 3.0 μm, PC Membrane, Translucent, TC, Sterile, 1/pk, 10/cs

726001 by NEST Scientific is a 100 mm cell culture insert-dish with a 3.0 μm polycarbonate (PC) membrane (75 mm insert, 44 cm² growth area), TC treated and E-beam sterilized to SAL 10⁻⁶. The translucent PC membrane supports migration/invasion assays, epithelial polarity studies, and co-culture systems; it is compatible with standard fixatives and fluorescent dyes. Supplied 1/pk, 10/case in peelable Tyvek packaging.

Surface Treatment

Pore Size (μm)

Membrane Material

Transparency

/Pack

/Case

Manufacturer

12 Cell Culture Inserts+12 Well Plate Specifications

12 Cell Culture Inserts+12 Well Plate, PET Membrane

724121 by NEST Scientific is a 12-well TC-treated PET membrane cell culture insert set (0.4 µm pore size), pre-loaded into a 12-well companion plate, sterile, 12/pk, 120/case. Supports epithelial barrier assays, TEER measurement, drug transport studies, and co-culture applications. Available through MBP, a registered vendor to Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, Eurofins, and many other institutes across the USA.

12 Cell Culture Inserts+12 Well Plate, 0.4 μm, PET Membrane, Opaque, TC, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+12 Well Plate, 1.0 μm, PET Membrane, Opaque, TC, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+12 Well Plate, 3.0 μm, PET Membrane, Translucent, TC, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+12 Well Plate, 8.0 μm, PET Membrane, Transparent, TC, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+12 Well Plate, 0.4 μm, PET Membrane, Opaque, Non-Treated, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+12 Well Plate, 1.0 μm, PET Membrane, Opaque, Non-Treated, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+12 Well Plate, 3.0 μm, PET Membrane, Translucent, Non-Treated, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+12 Well Plate, 8.0 μm, PET Membrane, Transparent, Non-Treated, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts + 12 Well Plate, PC Membrane Specifications

12 Cell Culture Inserts+12 Well Plate,PC Membrane

724101 by NEST Scientific — 12-well transwell insert system with 0.4 µm pore polycarbonate membrane inserts and matched 12-well plate. TC-treated, sterile, 12/pk. Suitable for permeability, TEER, and co-culture assays.

12 Cell Culture Inserts+12 Well Plate, 0.4 μm, PC Membrane, Opaque, TC, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+12 Well Plate, 3.0 μm, PC Membrane, Translucent, TC, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+12 Well Plate, 5.0 μm, PC Membrane, Translucent, TC, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+12 Well Plate, 8.0 μm, PC Membrane, Translucent, TC, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+12 Well Plate, 0.4 μm, PC Membrane, Opaque, Non-Treated, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+12 Well Plate, 3.0 μm, PC Membrane, Translucent, Non-Treated, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+12 Well Plate, 5.0 μm, PC Membrane, Translucent, Non-Treated, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+12 Well Plate, 8.0 μm, PC Membrane, Translucent, Non-Treated, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+24 Well Plate, PC Membrane Specifications

12 Cell Culture Inserts+24 Well Plate, PC Membrane

NEST Scientific 725101: 12 hanging cell culture inserts with 0.4 µm TC-treated polycarbonate (PC) membrane, pre-loaded in a 24-well companion plate. Sterile. Designed for transport, permeability, and barrier assays. Order at <a href="https://www.mbpinc.net/quickorder">mbpinc.net/quickorder</a>.

12 Cell Culture Inserts+24 Well Plate, 0.4 μm, PC Membrane, Opaque, TC, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+24 Well Plate, 3.0 μm, PC Membrane, Translucent, TC, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+24 Well Plate, 5.0 μm, PC Membrane, Translucent, TC, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+24 Well Plate, 8.0 μm, PC Membrane, Translucent, TC, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+24 Well Plate, 3.0 μm, PC Membrane, Translucent, Non-Treated, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+24 Well Plate, 8.0 μm, PC Membrane, Translucent, Non-Treated, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+24 Well Plate, 5.0 μm, PC Membrane, Translucent, Non-Treated, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+24 Well Plate, 0.4 μm, PC Membrane, Opaque, Non-Treated, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+24 Well Plate Specifications

12 Cell Culture Inserts+24 Well Plate, PET Membrane

725121 by NEST Scientific is a kit of 12 PET membrane cell culture inserts pre-seated in a 24-well receiver plate, with a 0.4 µm pore size, TC-treated surface, and sterile packaging. PET's lower fluorescence background makes these inserts preferred for FITC-dextran transport, fluorescence confocal, and TEER assays. Ships 12 inserts per kit, 48 kits per case.

12 Cell Culture Inserts+24 Well Plate, 0.4 μm, PET Membrane, Opaque, TC, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+24 Well Plate, 1.0 μm, PET Membrane, Opaque, TC, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+24 Well Plate, 3.0 μm, PET Membrane, Translucent, TC, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+24 Well Plate, 8.0 μm, PET Membrane, Transparent, TC, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+24 Well Plate, 0.4 μm, PET Membrane, Opaque, Non-Treated, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+24 Well Plate, 1.0 μm, PET Membrane, Opaque, Non-Treated, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+24 Well Plate, 3.0 μm, PET Membrane, Translucent, Non-Treated, Sterile, 12/pk, 48/cs

12 Cell Culture Inserts+24 Well Plate, 8.0 μm, PET Membrane, Transparent, Non-Treated, Sterile, 12/pk, 48/cs

PETG Bottle Accessories

<h2 dir="ltr">What are PETG Bottles with Accessories?</h2>
 PETG bottles with accessories are sterile, closed-system liquid storage and transfer assemblies combining a square PETG (polyethylene terephthalate glycol-modified) media bottle with a bi-directional transfer cap pre-fitted with TPE (thermoplastic elastomer) tubing and a terminal connector &mdash; Luer lock (male or female) or CPC (clamped pressure connector). The closed system allows aseptic media, serum, buffer, or pharmaceutical intermediate transfer between containers without open-pour steps, substantially reducing contamination risk and compliance burden in cGMP-regulated and BSL-2 laboratory environments.&nbsp; Choose PETG bottle assemblies for any liquid transfer workflow requiring an aseptic closed system &mdash; cGMP media dispensing, FBS aliquoting under sterile barrier, viral vector transfer, or pharmaceutical intermediate handling where open-pour steps are prohibited by SOP or regulatory requirement.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
1/4" ID TPE Tubing Systems with Female Luer Lock connectors
</li>
<li dir="ltr" aria-level="1">
1/4" ID TPE Tubing Systems with Male CPC connectors
</li>
<li dir="ltr" aria-level="1">
1/8" ID TPE Tubing Systems for precision fluid transfer
</li>
<li dir="ltr" aria-level="1">
1/8" ID TPE Tubing Systems with Female Luer Lock connectors
</li>
<li dir="ltr" aria-level="1">
1/8" ID TPE Tubing Systems with Male CPC connectors
</li>
<li dir="ltr" aria-level="1">
0.22&mu;m Vent Filter Assemblies for sterile pressure equalization
</li>
<li dir="ltr" aria-level="1">
Aseptic Transfer Caps with integrated sealing plugs
</li>
<li dir="ltr" aria-level="1">
Customizable Tubing Sets for closed-loop systems
</li>
</ul>
<h2 dir="ltr"> How to Choose PETG Bottle Assemblies</h2>
 Connector Type: Luer Lock vs. CPC Female Luer lock connectors (catalog 352503, 352506 series) mate with male Luer tips on syringe filters, tubing sets, and sterile sampling devices &mdash; the most common connector for inline sterile sampling and small-volume aseptic transfer. Male Luer lock connectors fit female Luer receptacles on peristaltic pump tubing and fluid path assemblies. CPC (clamped pressure connector, also called quick-connect) connectors provide a tool-free, dry-disconnect coupling between two fluid lines without dripping &mdash; used in bioreactor harvest lines, media feed assemblies, and bioprocess fluid path management. Tubing Size: 1/8" vs. 1/4" I.D. NEST assemblies are available with 60 cm TPE tubing in 1/8" I.D. &times; 1/4" O.D. (for low-flow, small-volume transfer with syringe or peristaltic pump at low speed) or 1/4" I.D. &times; 3/8" O.D. (for higher-flow transfer with standard peristaltic pumps). The 3/8" O.D. tubing is the standard for most bioprocess peristaltic pumps. Confirm pump head bore size against tubing O.D. before ordering. Bi-Directional vs. Standard Cap The bi-directional transfer cap in NEST PETG assemblies has a dual-port design: one port connects to the TPE transfer tubing (outflow or inflow path), and the cap as a whole seals the bottle neck during the non-transfer state. This design allows the bottle to function both as a source vessel (liquid out via tubing) and a receiver vessel (liquid in via tubing) without changing the cap assembly. Confirm whether your process requires one-way or two-way transfer before selecting the assembly format. Volume Options NEST PETG bottle assemblies are available in 250 mL, 500 mL, and 1,000 mL volumes, matching standard media, sera, and buffer kit sizes. The 250 mL assembly is standard for small-volume cGMP process sampling; 500 mL and 1,000 mL assemblies suit bulk media dispense and FBS aliquoting at scale. Standard Bottle vs. Assembly For applications that do not require tubing or connectors &mdash; routine cold storage of media, FBS thaw, and buffer archiving &mdash; standard NEST PETG square bottles with HDPE screw caps (catalog 352xxx without accessories) are available at a lower cost. The accessory assembly format is only necessary when closed-system transfer between containers is required.
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific PETG bottle assemblies are manufactured under a cGMP quality system conforming to ISO 13485 and 21 CFR 820 (QSR). All components are USP Class VI; the complete assembly is gamma irradiated in the USA to SAL 10⁻⁶ and provided in double-bagged sterile packaging for cleanroom entry. Each lot is tested and certified free of endotoxins, pyrogens, DNase, RNase, and particulates. PETG material: USP Class VI, temperature range &minus;80 &deg;C to +60 &deg;C; TPE tubing: USP Class VI, biocompatible, platinum-cured equivalent. The bi-directional cap body is polycarbonate (PC); the tubing and connector are TPE and polypropylene (PP) or nylon, all USP Class VI. As of 2026, NEST PETG bottle assemblies are used in cGMP biopharmaceutical manufacturing, viral vector production (lentivirus, AAV), FBS aliquoting under sterile barrier, and academic cGMP pilot programs. 
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Maintain the highest standards of aseptic integrity with our specialized assemblies. <a href="https://mbpinc.net/contact-us">Reach out to the experts</a> at Molecular Biology Products Inc. for a quote and keep your fluid handling error-free.

PETG Bottle With Accessories

Product Literature

1L PETG Square Storage Bottle Transfer Cap

C51121-BBB060B by NEST Scientific is a transfer cap for 1 L PETG square storage bottles. Pre-assembled with 60 cm weldable TPE tube (1/4" ID × 3/8" OD), Male CPC quick-connect connector with female sealing cap, and 0.22 µm vent filter (Φ24 mm). TPE is BPOG validated for extractables/leachables. Sterile, 1/pk, 10/cs. Bottle not included.

1L PETG Square Storage Bottle Transfer Cap, TPE Tube (60cm 1/4" ID 3/8" OD), Male CPC Connector with Female Sealing Cap, Vent Filter (0.22 μm Φ24mm), without Bottle, Sterile, 1/pk, 10/cs

1L PETG Square Storage Bottle Transfer Cap, TPE Tube (60cm 1/4" ID 3/8" OD), Female Luer Lock Connector with Luer plug, Vent Filter (0.22 μm Φ24mm), without Bottle, Sterile, 1/pk, 10/cs

1L PETG Square Storage Bottle Transfer Cap, TPE Tube (60cm 1/8" ID 1/4" OD), Male CPC Connector with Female Sealing Cap, Vent Filter (0.22 μm Φ24mm), without Bottle, Sterile, 1/pk, 10/cs

1L PETG Square Storage Bottle Transfer Cap, TPE Tube (60cm 1/8" ID 1/4" OD), Female Luer Lock Connector with Luer Plug, Vent Filter (0.22 μm Φ24mm), without Bottle, Sterile, 1/pk, 10/cs

1L PETG Square Storage Bottle 3-Port Transfer Cap, TPE Tube (60cm, 1/8" ID, 1/4" OD), Female Luer Connector with Male Sealing Plug, Vent Filter(0.22 µm, Membrane Area 4.5cm²), without Bottle, Sterile, 1/pk, 10/cs

PETG Square Bottles

<h2 dir="ltr">What are PETG Square Media Bottles?</h2>
 PETG square media bottles are single-use, sterile, square-footprint containers manufactured from glycol-modified polyethylene terephthalate (PETG) for storing tissue culture media, fetal bovine serum, PBS, HBSS, buffers, pharmaceutical intermediates, and pH-sensitive aqueous solutions. The square profile reduces cold-room and refrigerator footprint by approximately 25% compared to equivalent-volume round bottles, and flat sides prevent rolling during transport. PETG square bottles are the correct choice over PET when low-temperature stability to &minus;80 &deg;C is needed, when wall durability under cold-room handling is critical, or when closed-system accessory cap assemblies will be used for aseptic liquid transfer.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
30mL PETG Bottles &ndash; For micro-scale aliquots and stock sampling.
</li>
<li dir="ltr" aria-level="1">
60mL PETG Bottles &ndash; Compact size for small-volume buffer storage.
</li>
<li dir="ltr" aria-level="1">
125mL PETG Bottles &ndash; Standard size for routine media prep.
</li>
<li dir="ltr" aria-level="1">
250mL PETG Bottles &ndash; Ideal for general cell culture storage.
</li>
<li dir="ltr" aria-level="1">
500mL PETG Bottles &ndash; Optimized for mid-volume biological fluids.
</li>
<li dir="ltr" aria-level="1">
1000mL PETG Bottles &ndash; For bulk reagent storage and stock solutions.
</li>
<li dir="ltr" aria-level="1">
2000mL PETG Bottles &ndash; High-capacity for industrial-scale liquid handling.
</li>
</ul>
<h2 dir="ltr"> How to Choose PETG Square Media Bottles</h2>
 PETG vs. PET vs. PC PETG and PET are chemically similar but differ in crystallinity. PETG's glycol modification reduces polymer crystallinity, improving impact resistance, flexibility, and low-temperature stability. PETG is rated from &minus;80 &deg;C to +60 &deg;C; PET from &minus;60 &deg;C to +60 &deg;C; PC from &minus;135 &deg;C to +135 &deg;C. PETG and PET are both BPA-free and non-autoclavable. PC is autoclavable but BPA-containing. For low-temperature archival storage of serum, enzymes, and viral stocks below &minus;60 &deg;C, PETG is the appropriate single-use choice. Volume Selection by Application The 30 mL and 60 mL bottles suit single-use serum aliquots, enzyme dilutions, and small-scale media preparation for T25 or T75 flasks. The 125 mL and 250 mL formats cover standard T75 and T175 media changes. The 500 mL bottle aligns with standard FBS lot sizes and 500 mL media kit volumes. The 1,000 mL bottle stores a full 1 L media preparation or 1 L FBS aliquot. Each bottle includes 15% headspace above nominal volume for expansion during freezing and thawing. Graduated Markings: Molded vs. Printed NEST Scientific PETG square bottles feature molded graduations (not printed), which remain legible through repeated freeze-thaw cycles, solvent exposure, and autoclave label-remover use. Printed graduations on competing formats can abrade or smear over time. Molded graduations are an important quality checkpoint when selecting PETG bottles for long-term archival. Cap Design NEST PETG square bottle caps are HDPE, designed through a one-shot mold process without an inner gasket. The gasket-free design eliminates a potential leachable point and produces a tighter, more consistent seal under the torque applied during manual capping in a cold room or gloved biosafety cabinet environment. The external collar design provides a secure grip for easy sealing under gloves. Double-Bagged GMP Packaging NEST PETG square media bottles are available in double-bagged sterile packaging, meeting material entry requirements for GMP cleanrooms and ISO-classified bioprocess areas. Each inner bag is heat-sealed; the outer bag provides additional sterility protection against outer packaging damage during courier transit. Standard Bottle vs. Accessory Assembly For routine media and serum cold storage, standard PETG square bottles with HDPE screw caps are the appropriate, cost-effective format. When closed-system aseptic liquid transfer between containers is required &mdash; cGMP media dispensing, viral stock aliquoting, pharmaceutical intermediate handling &mdash; choose NEST PETG bottles with bi-directional transfer cap assemblies (TPE tubing + Luer lock or CPC connector). 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific PETG square media bottles are manufactured from 100% virgin PETG resin in ISO 8 (Class 10,000) cleanrooms. Both PET and PETG materials meet USP Class VI and USP &lt;661&gt; biocompatibility requirements and ISO 10993 quality system standards per NEST lot certificates. E-beam sterilization achieves SAL 10⁻⁶. Smooth inner surface minimizes liquid residue on the wall. Operating temperature: &minus;80 &deg;C to +60 &deg;C. HDPE cap is heavy-metal-free per regulatory testing. Shelf life: 3 years from production month. As of 2026, NEST Scientific is ISO 9001:2015 and ISO 13485 certified with FDA registration. 
In a field where a single leak can ruin weeks of data, precision is everything. <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">Contact the Molecular Biology Products Inc. team</a> for a quote and ensure your closed-system transfers stay flawless.

1L PETG Square Storage Bottle with Bi-directional Transfer Cap

C51121-BBB060A by NEST Scientific — 1L PETG square storage bottle with bi-directional transfer cap: TPE tube (60 cm, 1/4" ID), male CPC connector, and 0.22 µm vent filter. Sterile, 1/pk. For closed aseptic fluid transfer in bioprocess workflows.

1L PETG Square Storage Bottle with Bi-directional Transfer Cap with TPE Tube (60cm 1/4" ID 3/8" OD), Male CPC Connector with Female Sealing Cap, Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 10/cs

1L PETG Square Storage Bottle with Bi-directional Transfer Cap with TPE Tube (60cm 1/4" ID 3/8" OD), Female Luer Lock Connector with Luer Plug, Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 10/cs

1L PETG Square Storage Bottle with Bi-Directional Transfer Cap with TPE Tube (60cm 1/4" ID 7/16" OD), Heat seal ,Vent Filter (0.22 μm 4.5cm²), Sterile, 1/pk, 10/cs

1L PETG Square Storage Bottle with Bi-directional Transfer Cap with TPE Tube (60cm 1/8" ID 1/4" OD), Male CPC Connector with Female Sealing Cap, Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 10/cs

1L PETG Square Storage Bottle with Bi-Directional Transfer Cap with TPE Tube (60cm 1/8" ID 1/4" OD), Male Luer Connector with Female Luer Cap, Vent Filter (0.22 μm 4.5cm²), Sterile, 1/pk, 10/cs

1L PETG Square Storage Bottle with Bi-directional Transfer Cap with TPE Tube (60cm 1/8" ID 1/4" OD), Female Luer Lock Connector with Luer Plug, Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 10/cs

1L PETG Square Storage Bottle with 3-Port Transfer Cap with TPE Tube（60cm, 1/8" ID, 1/4" OD), Female Luer Connector with Male Sealing Plug, Vent Filter (0.22 µm, Membrane Area 4.5cm²), Sterile, 1/pk, 10/cs

250 mL PETG Square Storage Bottle with Bi-directional Transfer Cap

C50921-BBB060A by NEST Scientific is a 250 mL PETG square storage bottle with a bi-directional transfer cap, 60 cm TPE tube (1/4″ ID, 3/8″ OD), Female Luer Lock Connector, and 0.22 µm vent filter (Φ24 mm). BPOG-validated extractables, E-beam sterile, DNase/RNase/pyrogen-free, ISO 13485 and 21 CFR Part 820 compliant. 1/pk, 10/cs.

250mL PETG Square Storage Bottle with Bi-directional Transfer Cap with TPE Tube (60cm 1/4" ID 3/8" OD), Male CPC Connector with Female Sealing Cap, Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 10/cs

250mL PETG Square Storage Bottle with Bi-directional Transfer Cap with TPE Tube (60cm 1/4" ID 3/8" OD), Female Luer Lock Connector with Luer Plug, Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 10/cs

250mL PETG Square Storage Bottle with Bi-Directional Transfer Cap with TPE Tube (60cm 1/4" ID 7/16" OD), Heat seal ,Vent Filter (0.22 μm 4.5cm²), Sterile, 1/pk, 10/cs

250mL PETG Square Storage Bottle with Bi-directional Transfer Cap with TPE Tube (60cm 1/8" ID 1/4" OD), Male CPC Connector with Female Sealing Cap, Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 10/cs

250mL PETG Square Storage Bottle with Bi-directional Transfer Cap with TPE Tube (60cm 1/8" ID 1/4" OD), Female Luer Lock Connector with Luer Plug, Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 10/cs

250mL PETG Square Storage Bottle Transfer Cap

NEST Scientific C50921-BBB060B is a sterile 250 mL PETG square storage bottle with a transfer cap, 60 cm TPE tube (1/4″ ID × 3/8″ OD), female Luer lock connector, and 0.22 μm vent filter. BPOG E&L validated. For closed aseptic liquid transfer in pharmaceutical and bioprocess applications. Sterile, 1/pk, 10/cs.

250mL PETG Square Storage Bottle Transfer Cap, TPE Tube (60 cm 1/4" ID 3/8" OD), Male CPC Connector with Female Sealing Cap, Vent Filter (0.22 μm Φ24mm), without Bottle, Sterile, 1/pk, 10/cs

250mL PETG Square Storage Bottle Transfer Cap, TPE Tube (60cm 1/4" ID 3/8" OD), Female Luer Lock Connector with Luer Plug, Vent Filter (0.22 μm Φ24mm), without Bottle, Sterile, 1/pk, 10/cs

250mL PETG Square Storage Bottle Transfer Cap, TPE Tube (60cm 1/8" ID 1/4" OD), Male CPC Connector with Female Sealing Cap, Vent Filter (0.22 μm Φ24mm), without Bottle, Sterile, 1/pk, 10/cs

250mL PETG Square Storage Bottle Transfer Cap, TPE Tube (60cm 1/8" ID 1/4" OD), Female Luer Lock Connector with Luer Plug, Vent Filter (0.22 μm Φ24mm), without Bottle, Sterile, 1/pk, 10/cs

Erlenmeyer Flasks Accessories

<h2 dir="ltr">&nbsp;What are Erlenmeyer Flask Accessories?</h2>
 Erlenmeyer flask accessories are the replacement caps, transfer cap assemblies, vent filters, and adapter fittings used alongside PC and PETG shake flasks to customize gas exchange, enable closed-system liquid transfer, and replace consumable caps between culture runs. Accessory selection determines whether a shake flask acts as a standard culture vessel, a sealed storage container, or a closed-system liquid transfer assembly in a cGMP workflow.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li>Accessories compatible with high-efficiency Erlenmeyer flasks in 2 L, 3 L, and 5 L formats.</li>
<li>Available in bi-directional, inverted, and multifunctional transfer cap configurations.</li>
<li>Supports both standard-neck and wide-mouth 3 L flask designs.</li>
<li>Includes options with integrated silicon tubing for transfer applications.</li>
<li>Enables customization of flask workflows through interchangeable transfer cap accessories.</li>
</ul>
<h2 dir="ltr">&nbsp;</h2>
<h2 dir="ltr">How to Choose Erlenmeyer Flask Accessories</h2>
 Vented Caps: Standard vs. Oversized Membrane Standard vented Erlenmeyer caps have a 0.22 &micro;m PTFE membrane in a small-diameter HDPE housing. NEST Scientific vented caps feature an oversized membrane area that increases the gas exchange surface, supporting higher oxygen mass transfer for dense suspension cultures at elevated shaker speeds. At 150 RPM with 20&ndash;25% fill, NEST oversized vented caps provide sufficient oxygen transfer for mammalian suspension cultures at densities up to approximately 5 &times; 10⁶ cells/mL. For cultures targeting densities above 10 &times; 10⁶ cells/mL in a standard shake flask format, a sparged bioreactor system is more appropriate. Plug Seal Caps: Autoclave vs. Non-Autoclave HDPE plug seal caps for NEST PC Erlenmeyer flasks are compatible with autoclave cycles at 121 &deg;C when loosened 1&ndash;1.5 turns before autoclaving to prevent steam pressure buildup. Plug seal caps for NEST PETG flasks are compatible with static incubation and transport but not autoclaving (PETG maximum temperature: +60 &deg;C). Confirm whether your accessory cap is rated for autoclave use before including it in a steam sterilization cycle. Transfer Cap Assemblies for Closed-System Culture NEST customized transfer cap assemblies combine an Erlenmeyer-compatible cap body with a bi-directional transfer port, TPE tubing, and a Luer lock or CPC connector &mdash; enabling aseptic inoculation, sampling, or media addition to a running shake flask culture without opening the flask. This closed-system capability is used in cGMP process development, GMP seed train management, and contamination-critical research workflows. Transfer caps are available in sizes matching NEST 125 mL, 250 mL, 500 mL, 1,000 mL, 2,000 mL, and 3,000 mL flask neck formats. Cap Sizing and Flask Compatibility Erlenmeyer flask caps are sized to the neck thread diameter of the flask. NEST Scientific PC and PETG Erlenmeyer flasks use proprietary screw-thread neck dimensions &mdash; confirm the specific cap catalog number matches your flask's neck size before ordering replacement caps. Do not mix cap brands across flask brands without verifying thread compatibility; mismatched caps create gas leaks at the vent membrane, contaminating cultures. Replacement Cap Frequency for Reusable PC Flasks For reusable PC flasks, replace the vented cap after each culture run to prevent membrane clogging from media splatter, condensation, and biofilm formation on the PTFE membrane. Reusing a contaminated or clogged vent membrane reduces gas exchange efficiency, creating a DO deficit that depresses cell growth without a visible contamination event. NEST vented caps for PC flasks are available in bulk packs for cost-efficient per-run replacement. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific Erlenmeyer flask accessories &mdash; including replacement vented caps, plug seal caps, and transfer cap assemblies &mdash; are manufactured from USP Class VI HDPE (cap housing) and USP Class VI PTFE (0.22 &micro;m membrane), with TPE tubing in transfer assemblies. All accessory caps are sterile and non-pyrogenic per the lot certificate. The 0.22 &micro;m membrane pore size is the standard for microbial retention in cell culture venting applications. Transfer cap assemblies compatible with the NEST Erlenmeyer flask line are available in sizes for 125 mL through 3,000 mL flasks, individually wrapped in sterile packaging. NEST Scientific is ISO 9001:2015 and ISO 13485 certified.&nbsp; 
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Erlenmeyer Flask Accessories

2L High Efficiency Erlenmeyer Flask Bi-directional Transfer Cap

C10122-AZB050B by NEST Scientific is a bi-directional liquid transfer cap for 2 L Erlenmeyer flasks featuring a 50 cm TPE tube (1/8" ID), 0.22 µm hydrophobic PTFE vent filter, and a Colder Products MPC connector. E-beam sterilized to SAL 10⁻⁶ per ISO 11137; supplied 1/sterile bag, 4/cs. Flask not included.

2L High Efficiency Erlenmeyer Flask Bi-directional Transfer Cap with TPE Tube (50cm 1/8" ID 1/4" OD), Vent Filter (0.22 μm Φ24mm), without Flask , Sterile, 1/pk, 4/cs

2L High Efficiency Erlenmeyer Flask Bi-directional Transfer Cap with TPE Tube (50cm 1/4" ID 3/8" OD), Vent Filter (0.22 μm Φ24mm), without Flask, Sterile, 1/pk, 4/cs

2L High Efficiency Erlenmeyer Flask Bi-directional Transfer Cap with Silicon Tube (50cm1/4" ID 3/8" OD), Male CPC Connector with Female Sealing Cap with, Vent Filter (0.22 μm Φ42mm), without Flask, Sterile, 1/pk, 4/cs

2L High Efficiency Erlenmeyer Flask Bi-directional Transfer Cap with Silicon Tube (50cm1/4" ID 3/8" OD), Female CPC Connector with Male Sealing Cap with, Vent Filter (0.22 μm Φ42mm), without Flask, Sterile, 1/pk, 4/cs

2L High Efficiency Erlenmeyer Flask Inverted Transfer Cap

NEST Scientific C10131-AZC050B is a sterile inverted transfer cap for 2 L High Efficiency Erlenmeyer Flasks, featuring a 50 cm TPE tube (1/8″ ID × 1/4″ OD) and a 0.22 μm vent filter for closed-system liquid transfer in bioprocess and cell culture applications. Sold without flask; 1/pk, 4/cs.

2L High Efficiency Erlenmeyer Flask Inverted Transfer Cap with TPE Tube (50cm 1/8" ID 1/4" OD), Vent Filter (0.22 μm Φ42mm), without Flask, Sterile, 1/pk, 4/cs

2L High Efficiency Erlenmeyer Flask Inverted Transfer Cap with TPE Tube (50cm 1/4" ID 7/16" OD), Vent Filter (0.22 μm Φ42mm), without Flask , Sterile, 1/pk, 4/cs

2L High Efficiency Erlenmeyer Flask Multifunctional Transfer Cap

C10111-AJE052B by NEST Scientific is a multifunctional transfer cap for 2 L High Efficiency Erlenmeyer flasks. Pre-assembled with a 52 cm TPE tube (1/8" ID × 1/4" OD), T-junction Luer lock sampling nozzle, and 0.22 µm PTFE vent filter (Φ50 mm). E-beam sterilized to SAL 10⁻⁶. Cap only, sterile, 1/pk, 4/cs.

2L High Efficiency Erlenmeyer Flask Multifunctional Transfer Cap with TPE Tube (52cm 1/8" ID 1/4" OD) and a Sampling Nozzle, Vent Filter (0.22 μm Φ50mm), without Flask, Sterile, 1/pk, 4/cs

2L High Efficiency Erlenmeyer Flask Multifunctional Transfer Cap with TPE Tube ( 50cm 1/4" ID 7/16" OD), Vent Filter (0.22 μm Φ24mm), without Flask, Sterile, 1/pk, 4/cs

2L High Efficiency Erlenmeyer Flask Multifunctional Transfer Cap with Silicon Tube (100cm 3/16" ID 3/8" OD), Male CPC Connector with Female Sealing Cap, Vent Filter (0.22 μm Φ24mm), without Flask, Sterile, 1/pk, 4/cs

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