Name: PET Square Media Bottles, Sterile
SKU: 354611
Availability: InStock

Media Storage Bottles

<h2 dir="ltr">What are Media Storage Bottles?</h2>
 Media storage bottles for cell culture are sterile, single-use or multi-use plastic containers used to store and dispense tissue culture media, sera, buffers, and other aqueous solutions in research and biopharmaceutical laboratory settings. Choose PC bottles for autoclavable reuse; PET for single-use media storage and transport; PETG for low-temperature archival, closed-system transfer, and pH-sensitive long-term storage.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
<a href="media-storage-bottles/petg-square-bottles">PETG Square Bottles </a>for space-saving laboratory storage
</li>
<li dir="ltr" aria-level="1">
<a href="consumables/cell-culture/media-storage-bottles/petg-bottle-with-accessories" style="color: #236fa1;">PETG Bottle With</a><a href="media-storage-bottles/petg-bottle-with-accessories" style="color: #236fa1;"> Accessories&nbsp;</a><a href="https://mbpinc.net/petg-bottle-with-accessories" title="PETG Bottle With Accessories" style="color: #236fa1; text-decoration: underline;"></a>for specialized fluid handling
</li>
<li dir="ltr" aria-level="1">
<a href="media-storage-bottles/pc-media-bottles">PET Media Bottles </a>for high-clarity reagent containment
</li>
<li dir="ltr" aria-level="1">
<a href="media-storage-bottles/pc-media-bottles">PC Media Bottles </a>for superior mechanical durability
</li>
</ul>
<h2 dir="ltr"> How to Choose Media Storage Bottles</h2>
 Material: PC vs. PET vs. PETG PC (polycarbonate) bottles are window-clear, shatter-proof, and rated for repeated autoclaving (up to 5&ndash;10 cycles) &mdash; the standard for labs that prepare and sterilize media in-house. PC is resistant to alcohols, weak acids, and aliphatic hydrocarbons; it is not compatible with strong bases or organic solvents. PC contains BPA (bisphenol A) by its monomer chemistry and is not recommended for long-term hormone-sensitive cell culture applications. PET bottles are optically clear, lighter than glass, non-cytotoxic, and non-autoclavable, rated from &minus;60 &deg;C to +60 &deg;C. They are the cost-effective standard for single-use media and sera storage in non-autoclave workflows. PETG (glycol-modified PET) adds a glycol co-monomer that reduces crystallinity, improving impact resistance, low-temperature stability to &minus;80 &deg;C, and flexibility. PETG is the preferred material for long-term storage of pH-sensitive liquids, protein/vaccine intermediates, and applications requiring closed-system accessory assemblies. Round vs. Square Bottles Round bottles (Boston round, GL45 thread) are compatible with standard bottle-top vacuum filtration systems (Millipore Stericup, Corning Costar) and are the format of choice for media vacuum-filtered in-house. Square bottles conserve refrigerator and cold room shelf space due to their flat sides and stackable design, reducing storage footprint by approximately 25% versus equivalent-volume round bottles. NEST Scientific PET and PETG square media bottles feature molded graduations and a white labeling area on two sides. Volume Range Standard media bottle volumes are 60 mL, 125 mL, 250 mL, 500 mL, and 1,000 mL, corresponding to common media and sera kit sizes. A 250 mL bottle accommodates a standard 250 mL media aliquot; a 500 mL bottle holds standard FBS lots or 500 mL media packs. Confirm that the bottle's nominal volume includes 15% additional headspace for mixing, thawing expansion, and transfer operations. Accessory Assembly vs. Standard Cap Standard PETG square media bottles ship with an HDPE screw cap &mdash; suitable for storage and manual transfer. PETG bottles with accessory caps (bi-directional transfer cap + TPE tubing + Luer lock or CPC connector, NEST catalog 3525xx series) create a fully closed aseptic liquid transfer system &mdash; used in cGMP environments, bioprocess research, and any workflow where open-pour media transfer creates contamination or regulatory risk. Sterility and Certifications For cell culture use, all media storage bottles must be certified DNase/RNase-free, non-pyrogenic (endotoxin-free), and non-cytotoxic. NEST Scientific PET and PETG media bottles meet USP Class VI and ISO 10993 biocompatibility requirements, are manufactured in ISO 8 cleanrooms, and are gamma or E-beam-sterilized. PC bottles from autoclave-capable suppliers use virgin Lexan&reg; PC resin compliant with USP Class VI.
&nbsp;
<h2 dir="ltr">Specifications Context</h2>
 NEST Scientific square PET media bottles (catalog 334xxx) and PETG media bottles (catalog 352xxx) are manufactured from 100% virgin material in ISO 8 cleanrooms. Both materials meet USP Class VI and ISO 10993 biocompatibility requirements. Bottles are gamma irradiated in the USA or E-beam sterilized to SAL 10⁻⁶; each lot is certified DNase/RNase-free, pyrogen-free, and endotoxin-free. PETG bottles feature molded graduations (not printed), smooth inner surface to minimize residue on the wall, a one-shot-molded cap seat (no inner gasket required), and a double-bagged sterile packaging format meeting GMP purification workshop material-entry requirements. NEST Scientific PET and PETG media bottles are stocked by MBP in sizes from 60 mL to 1,000 mL. 
Don't wait for a contamination crisis to upgrade your storage. <a href="https://mbpinc.net/contact-us" title="Reach out to the Molecular Biology Products Inc.">Reach out to the Molecular Biology Products Inc.</a> experts for a quote and keep your reagents locked down.

<h2 dir="ltr">What are PET Media Bottles?</h2>
 PET (polyethylene terephthalate) media storage bottles are single-use, sterile, optically clear plastic containers for storing tissue culture media, fetal bovine serum, PBS, HBSS, trypsin-EDTA, and other aqueous laboratory solutions. PET is manufactured by an injection stretch-blow molding (ISBM) process that produces glass-like optical clarity, low gas permeability (especially to CO₂ and O₂ compared to polystyrene), excellent mechanical strength, and a smooth inner surface that minimizes liquid residue. PET bottles are BPA-free, non-autoclavable, and rated for use from &minus;60 &deg;C to +60 &deg;C.
&nbsp;They are the dominant single-use format for media and sera storage in cell culture because of their glass-comparable clarity, low extractable profile, and cost-effectiveness compared to borosilicate glass. Choose PET media bottles as the default single-use format for ready-to-use media and sera storage, cold room archiving, and any application where autoclaving is not required.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
60mL PET Media Bottles for small-scale sampling
</li>
<li dir="ltr" aria-level="1">
125mL Sterile PET Bottles for routine reagent handling
</li>
<li dir="ltr" aria-level="1">
250mL PET Storage Bottles for mid-range media volumes
</li>
<li dir="ltr" aria-level="1">
500mL/0.5L PET Bottles for standard laboratory preparations
</li>
<li dir="ltr" aria-level="1">
1000mL/1L PET Media Bottles for large-batch stock solutions
</li>
<li dir="ltr" aria-level="1">
2L High-Volume PET Bottles for industrial-scale storage
</li>
<li dir="ltr" aria-level="1">
5L PET Media Vessels for bulk liquid containment
</li>
<li dir="ltr" aria-level="1">
Double-layer Packaged Bottles for enhanced cleanroom safety
</li>
<li dir="ltr" aria-level="1">
Square Shoulder Tray-Packed Bottles for secure, organized transport
</li>
</ul>
<h2 dir="ltr"> How to Choose PET Media Bottles</h2>
 Round vs. Square Format Round PET media bottles (Boston round, GL45) are directly compatible with bottle-top vacuum filtration systems and standard lab bottle accessories. Square PET media bottles (NEST Scientific catalog 334xxx) feature flat sides that stack efficiently in refrigerators and cold rooms, reducing footprint by approximately 25% versus round bottles and preventing rolling when stored horizontally. NEST PET square bottles have molded graduations (not printed), a white labeling area on two sides, and embossed markings for durable identification. Volume Selection PET media bottles are available in 30 mL, 60 mL, 125 mL, 250 mL, 500 mL, and 1,000 mL volumes. A 500 mL bottle accommodates a standard 500 mL aliquot of cell culture media (DMEM, RPMI, MEM) or a standard FBS lot aliquot. The 1,000 mL bottle holds a full 1 L media preparation. Each bottle includes approximately 15% additional headspace above the nominal volume for thawing expansion (&minus;20 &deg;C frozen FBS thaw) and transfer convenience. Cap Material NEST Scientific PET media bottle caps are made from HDPE (high-density polyethylene), free of heavy metals per regulatory testing. The cap is designed through a one-shot mold process without an inner gasket, fitting securely onto the bottle neck with enhanced sealing performance &mdash; an important feature for leak-proof horizontal storage and air transport. Gas Barrier Properties PET and PETG both reduce gas permeability to CO₂ and O₂ compared to polystyrene, maintaining medium pH and preventing dissolved gas equilibration during storage. For pH-sensitive media containing bicarbonate buffers, PET and PETG are materially superior to polystyrene bottles, which allow CO₂ outgassing and pH shift during storage at room temperature or 4 &deg;C. Sterility and Lot Traceability NEST Scientific PET media bottles are gamma irradiated or E-beam sterilized; each lot is certified DNase/RNase-free, human DNA-free, pyrogen-free, and non-cytotoxic per lot certificate. Lot numbers are printed on each bottle and packaging for traceability. Shelf life is 3 years from the production month per NEST lot release data. PET bottles meet FDA requirements for materials used in contact with sterile liquid pharmaceuticals. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific PET square media bottles are manufactured from 100% virgin PET resin via the ISBM process in ISO 8 cleanrooms. Material meets USP Class VI and ISO 10993 biocompatibility, FDA requirements, and USP &lt;661&gt; container testing for plastics in contact with sterile solutions. Operating temperature: &minus;60 &deg;C to +60 &deg;C; not autoclavable. Smooth inner wall minimizes residue; molded graduations on two sides. The HDPE cap is heavy-metal-free. Packaging is double-bagged in sterile format for GMP-compatible cleanroom entry. Shelf life: 3 years from production month. As of 2026, NEST Scientific PET media bottles are stocked by MBP in the full size range. 
&nbsp;
Don&rsquo;t wait for a compromised sample to upgrade your containment. <a href="https://mbpinc.net/contact-us">Contact now </a>and request a quote from Molecular Biology Products Inc. and keep your lab results (and your PI) stress-free.

PET Media Bottles

DataSheet

Product Literature

PET Square Media Bottles (Sterile)

PET Square Media Bottles, Sterile

Nest Scientific sterile PET square media bottles — optically clear, 60 mL to 1 L, not autoclavable (use PC series 354313 if autoclave required). Seven SKUs: 354611/353611/352611 (60/125/250 mL), 333001/333621 (500 mL standard and square shoulder), 334001 (1 L), 333624 (500 mL square shoulder, tray-packed, 80/cs).

Manufacturer

60mL PET Square Media Bottles, Sterile, 6/pk, 48/cs

125mL PET Square Media Bottles, Sterile, 6/pk, 48/cs

250mL PET Square Media Bottles, Sterile, 6/pk, 48/cs

500mL PET Square Media Bottles, Sterile, 8/pk, 24/cs

500mL PET Square Media Bottless, Square Shoulder Style, Sterile, 8/pk, 24/cs

1000mL PET Square Media Bottles, Sterile, 4/pk, 12/cs

500 mL PET Square Media Bottles, Square Shoulder Style, Tray-Packed, Sterile, 20/pk, 80/cs

United States

Texas

mbpinc