Name: Nest Scientific PC Square Media Bottles, Sterile
SKU: 354314
Availability: InStock

Media Storage Bottles

<h2 dir="ltr">What are Media Storage Bottles?</h2>
 Media storage bottles for cell culture are sterile, single-use or multi-use plastic containers used to store and dispense tissue culture media, sera, buffers, and other aqueous solutions in research and biopharmaceutical laboratory settings. Choose PC bottles for autoclavable reuse; PET for single-use media storage and transport; PETG for low-temperature archival, closed-system transfer, and pH-sensitive long-term storage.
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<h2 dir="ltr">What you will find:</h2>
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<a href="media-storage-bottles/petg-square-bottles">PETG Square Bottles </a>for space-saving laboratory storage
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<a href="consumables/cell-culture/media-storage-bottles/petg-bottle-with-accessories" style="color: #236fa1;">PETG Bottle With</a><a href="media-storage-bottles/petg-bottle-with-accessories" style="color: #236fa1;"> Accessories&nbsp;</a><a href="https://mbpinc.net/petg-bottle-with-accessories" title="PETG Bottle With Accessories" style="color: #236fa1; text-decoration: underline;"></a>for specialized fluid handling
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<a href="media-storage-bottles/pc-media-bottles">PET Media Bottles </a>for high-clarity reagent containment
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<a href="media-storage-bottles/pc-media-bottles">PC Media Bottles </a>for superior mechanical durability
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<h2 dir="ltr"> How to Choose Media Storage Bottles</h2>
 Material: PC vs. PET vs. PETG PC (polycarbonate) bottles are window-clear, shatter-proof, and rated for repeated autoclaving (up to 5&ndash;10 cycles) &mdash; the standard for labs that prepare and sterilize media in-house. PC is resistant to alcohols, weak acids, and aliphatic hydrocarbons; it is not compatible with strong bases or organic solvents. PC contains BPA (bisphenol A) by its monomer chemistry and is not recommended for long-term hormone-sensitive cell culture applications. PET bottles are optically clear, lighter than glass, non-cytotoxic, and non-autoclavable, rated from &minus;60 &deg;C to +60 &deg;C. They are the cost-effective standard for single-use media and sera storage in non-autoclave workflows. PETG (glycol-modified PET) adds a glycol co-monomer that reduces crystallinity, improving impact resistance, low-temperature stability to &minus;80 &deg;C, and flexibility. PETG is the preferred material for long-term storage of pH-sensitive liquids, protein/vaccine intermediates, and applications requiring closed-system accessory assemblies. Round vs. Square Bottles Round bottles (Boston round, GL45 thread) are compatible with standard bottle-top vacuum filtration systems (Millipore Stericup, Corning Costar) and are the format of choice for media vacuum-filtered in-house. Square bottles conserve refrigerator and cold room shelf space due to their flat sides and stackable design, reducing storage footprint by approximately 25% versus equivalent-volume round bottles. NEST Scientific PET and PETG square media bottles feature molded graduations and a white labeling area on two sides. Volume Range Standard media bottle volumes are 60 mL, 125 mL, 250 mL, 500 mL, and 1,000 mL, corresponding to common media and sera kit sizes. A 250 mL bottle accommodates a standard 250 mL media aliquot; a 500 mL bottle holds standard FBS lots or 500 mL media packs. Confirm that the bottle's nominal volume includes 15% additional headspace for mixing, thawing expansion, and transfer operations. Accessory Assembly vs. Standard Cap Standard PETG square media bottles ship with an HDPE screw cap &mdash; suitable for storage and manual transfer. PETG bottles with accessory caps (bi-directional transfer cap + TPE tubing + Luer lock or CPC connector, NEST catalog 3525xx series) create a fully closed aseptic liquid transfer system &mdash; used in cGMP environments, bioprocess research, and any workflow where open-pour media transfer creates contamination or regulatory risk. Sterility and Certifications For cell culture use, all media storage bottles must be certified DNase/RNase-free, non-pyrogenic (endotoxin-free), and non-cytotoxic. NEST Scientific PET and PETG media bottles meet USP Class VI and ISO 10993 biocompatibility requirements, are manufactured in ISO 8 cleanrooms, and are gamma or E-beam-sterilized. PC bottles from autoclave-capable suppliers use virgin Lexan&reg; PC resin compliant with USP Class VI.
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<h2 dir="ltr">Specifications Context</h2>
 NEST Scientific square PET media bottles (catalog 334xxx) and PETG media bottles (catalog 352xxx) are manufactured from 100% virgin material in ISO 8 cleanrooms. Both materials meet USP Class VI and ISO 10993 biocompatibility requirements. Bottles are gamma irradiated in the USA or E-beam sterilized to SAL 10⁻⁶; each lot is certified DNase/RNase-free, pyrogen-free, and endotoxin-free. PETG bottles feature molded graduations (not printed), smooth inner surface to minimize residue on the wall, a one-shot-molded cap seat (no inner gasket required), and a double-bagged sterile packaging format meeting GMP purification workshop material-entry requirements. NEST Scientific PET and PETG media bottles are stocked by MBP in sizes from 60 mL to 1,000 mL. 
Don't wait for a contamination crisis to upgrade your storage. <a href="https://mbpinc.net/contact-us" title="Reach out to the Molecular Biology Products Inc.">Reach out to the Molecular Biology Products Inc.</a> experts for a quote and keep your reagents locked down.

<h2>What are PC Media Bottles?</h2>
 PC (polycarbonate) media storage bottles are reusable, autoclavable, highly transparent plastic containers used to prepare, sterilize, store, and dispense tissue culture media, sera, buffers, and other aqueous solutions. Polycarbonate &mdash; a rigid aromatic polyester with carbonate linkages &mdash; is the strongest standard thermoplastic, rated for use from &minus;135 &deg;C to +135 &deg;C, making it suitable for ultra-low freezer storage through repeated autoclave sterilization at 121 &deg;C. PC is window-clear, virtually shatterproof compared to glass, and dimensionally stable after autoclaving when not placed under vacuum stress. PC media bottles are the correct choice for any in-house media or buffer preparation workflow requiring autoclave sterilization, where cost amortization across multiple autoclave cycles justifies the higher per-bottle price versus single-use PET/PETG.
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<h2 dir="ltr">What you will find:</h2>
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30mL PC Media Bottles for small-scale reagent sampling
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60mL PC Media Bottles for compact laboratory storage
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125mL Polycarbonate Bottles for routine buffer handling
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250mL PC Media Bottles for mid-range media volumes
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500mL PC Media Bottles for standard cell culture prep
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1000mL PC Media Bottles for large-batch reagent storage
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2000mL Large Capacity Bottles for high-volume liquid transfer
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5000mL PC Media Bottles for industrial-scale stock solutions
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<h2> How to Choose PC Media Bottles</h2>
 Number of Autoclave Cycles Standard PC lab bottles withstand 5&ndash;10 autoclave cycles at 121 &deg;C before mechanical strength begins to degrade, and discoloration may appear. Nalgene polycarbonate bottles (USP Class VI virgin resin, Thermo Fisher) are rated for use up to the manufacturer's cycle guidance. Each autoclave cycle causes mild hydrolytic attack on carbonate linkages &mdash; do not use PC bottles for vacuum applications after they have been autoclaved, as mechanical strength reduction raises implosion risk. BPA Consideration Polycarbonate contains bisphenol A (BPA) as a consequence of its carbonate-monomer chemistry. Trace BPA can leach from PC surfaces into aqueous solutions, particularly at elevated temperatures (autoclaving, warm media). For applications using estrogen receptor&ndash;positive cell lines (MCF-7, T47D), primary endocrine cells, or embryonic stem cells, BPA-free alternatives (PET or PETG) are the safer choice. For standard media preparation and non-hormone-sensitive applications, the trace BPA levels in USP Class VI PC do not affect results. Chemical Compatibility PC resists weak acids, alcohols (ethanol up to 70%), and aliphatic hydrocarbons. PC is not compatible with strong bases (NaOH, KOH), organic solvents (acetone, DMSO at high concentration), concentrated acids, or hydrolytic conditions at elevated pH &mdash; all rapidly degrade carbonate linkages, causing crazing, cracking, or failure. Never autoclave PC with alkaline or oxidizing cleaning agents. For compatibility questions, consult the manufacturer's chemical resistance guide. Round (GL45) vs. Square Format PC round bottles (Boston round profile, GL45 threaded neck) are the dominant format for media preparation because they directly accept bottle-top vacuum filtration assemblies (Millipore Stericup, Corning) and are compatible with GL45 lids and pouring rings. PC square bottles conserve refrigerator shelf space and are preferred for cold-storage media archiving. Confirm whether your bottle-top filter threading matches GL45, 38-430, or 45-400 before ordering round bottles. Volume and Headspace PC media bottles are available from 125 mL to 10 L. Standard cell culture volumes &mdash; 125 mL, 250 mL, 500 mL, 1,000 mL &mdash; correspond to common media kit sizes. Each bottle nominally includes 15% additional headspace for autoclaving thermal expansion (liquid expands ~4% on autoclave heating) and safe pouring; never fill above the marked maximum fill line before autoclaving. 
<h2> Specifications Context</h2>
 Nalgene polycarbonate bottles are manufactured from USP Class VI laboratory-grade PC resin compliant with USP and ASTM biocompatibility testing, EP monographs, EU food-contact directives, CONEG, and RoHS requirements. Temperature use range: &minus;135 &deg;C to +135 &deg;C; autoclavable at 121 &deg;C per cycle guidance. Mechanical properties: PC has a tensile strength of approximately 55&ndash;75 MPa and impact resistance that remains &gt;50 J/m at &minus;40 &deg;C, making it effectively shatterproof under typical lab handling. After autoclaving, PC retains clarity but loses some tensile strength with each cycle &mdash; monitor for crazing, discoloration, or cracks that indicate end-of-service-life. As of 2026, MBP carries PC media bottles suitable for autoclave-based media preparation workflows.
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<a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">Reach out to the Molecular Biology Products Inc.</a> experts for a quote and upgrade your storage standards today.

PC Media Bottles

Nest Scientific PC Square Media Bottles, Sterile

Nest Scientific PC Square Media Bottles, Sterile are polycarbonate (PC) square media bottles, enzyme-free and double-bagged, for cell culture, molecular biology, genomics, and proteomics. Autoclavable at 121 °C; manufactured under ISO 13485:2016 and ISO 9001. Available in 30mL (354314), 60mL (354714), 125mL (353314), 250mL (352314), 500mL (333314), 1000mL (334314), and 5L (355714).

Manufacturer

30mL PC Square Media Bottles, Sterile, double bagged small pacakging, 5/pk, 40/cs