Tumor cells

Thawing Protocol

Cell Line Catalog (Volume 2)

T8290 UWO37 (HPV16) Cells

UWO37 (HPV16) Cells (T8290) by MBP are derived from oral tongue cancer and express HPV16. Model for investigating HPV and head and neck squamous cell carcinoma. Split ratio 1:2–1:3, PDT 37–45h. BSL II. 1&times;106 cells / 1.0 mL, frozen. MTA required.

T8292 HN12 Cells

T8292 HN12 Cells are human metastatic HNSCC cells expressing truncated p53 protein, used for apoptosis and therapeutic pathway research in head and neck squamous cell carcinoma. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.

T8293 U2OS Cells

T8293 by Applied Biological Materials (ABM) is a CRISPR/Cas9 HMGN1-knockout U2OS osteosarcoma line. The parental U2OS carries wild-type p53, wild-type Rb, and is p16-negative (CDKN2A-null) &mdash; a well-characterized CDK pathway background. Suited for chromatin biology, DNA damage, CDK4/6 inhibitor, and osteosarcoma drug-screening applications.

T8319 MELA15 Cells

T8319 by Applied Biological Materials (ABM) is MELA15, a human melanoma cell line from an inguinal lymph node metastasis of a 39-year-old Caucasian female. BRAF wild-type with confirmed NRAS Q61R (61 CAA&rarr;CGA) and PI3KCA wild-type &mdash; an NRAS-mutant profile relevant for MEK inhibitor studies and BRAF-wt negative controls in drug screening panels. Population doubling time is ~30 hours.

T8320 BCJ-4T Cells

T8320 BCJ-4T Cells are human bladder urothelial (transitional cell) carcinoma cells expressing amelogenin as a potential biomarker, with ~29.2 hr doubling time. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.

T8321 Human Adrenocortical HAC13 Cells

T8321 HAC13 Cells are a clonal NCI-H295R-derived human adrenocortical carcinoma line responsive to all three main adrenocortical regulators: angiotensin II/K+ (aldosterone) and cAMP agonists (cortisol/DHEA). Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.

T8322 Human Adrenocortical HAC15 Cells

T8322 HAC15 Cells are a clonal NCI-H295R-derived human adrenocortical carcinoma line responsive to all three main adrenocortical regulators, including a unique modest cortisol increase with chronic ACTH treatment alongside angiotensin II/K+ (aldosterone) and cAMP agonist (cortisol/DHEA) responses. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.

T8323 Human Adrenocortical HAC50 Cells

T8323 HAC50 Cells are a clonal NCI-H295R-derived human adrenocortical carcinoma line functionally responsive to all three main adrenocortical regulators: angiotensin II, potassium (aldosterone), and cAMP agonists (cortisol/DHEA). Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.

T8324 A2243 Cells

T8324 A2243 Cells are a human synovial sarcoma cell line from a 12-year-old female, carrying the t(X;18) chromosomal translocation resulting in SYT-SSX fusion, the defining molecular event of synovial sarcoma. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.

T8325 Rheumatoid Arthritis EBV+ Cells (Carejavi-01)

T8325 Carejavi-01 Cells are EBV-positive B-lymphocyte cells from a male rheumatoid arthritis patient, expressing CD19, CD20, CD38, CD23, and CD58, used to study EBV's role in RA pathogenesis. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.

T8326 UM-HACC-2A Cells

T8326 UM-HACC-2A Cells are a human adenoid cystic carcinoma cell line with MYB:NFIB fusion, cisplatin resistance, and metastatic potential; transplantable in the submandibular gland; 2D and 3D culture-compatible; STR-profiled. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.

T8327 OC3 Cells

OC3 Cells (T8327) by MBP are derived from oral carcinoma with characteristic chromosomal losses (4q, 6p, 8p), gains (1q, 5q, 8q, chr20), and high p53 expression. Used for areca-associated genetic research in oral keratinocytes. PDT 24h, split 1:6, seeding 15,000–20,000/cm². BSL II. 1&times;106 cells / 1.0 mL, frozen. MTA required.