Tumor cells

Thawing Protocol

Cell Line Catalog (Volume 2)

T8329 Penl2 Cells

T8329 Penl2 Cells are human penile squamous cell carcinoma cells with strong invasion potential, tumorigenic in mice, cisplatin-sensitive, anti-EGFR-resistant, and androgen-unresponsive, used to study penile cancer metastasis mechanisms. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.

T8330 149RM Cells

T8330 149RM Cells are human penile squamous cell carcinoma cells, tumorigenic in mice, cisplatin-sensitive, anti-EGFR-resistant, and androgen-unresponsive. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; BSL-2; RUO. Available in China only. MTA required.

T8331 149Rca Cells

T8331 149Rca Cells are human penile squamous cell carcinoma cells that are tumorigenic in mice, cisplatin-sensitive, anti-EGFR-resistant, and androgen-unresponsive, with high proliferation and metastasis potential. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; BSL-2; RUO. Available in China only. MTA required.

T8333 K14D Cells

T8333 K14D Cells are a low-malignancy rat esophageal squamous cell carcinoma cell line from Wistar rats, non-metastatic and non-transplantable in nude mice. Useful as a low-grade ESCC comparator. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.

T8334 ESCC-DR Cells

T8334 ESCC-DR Cells are a rat esophageal squamous cell carcinoma cell line derived from a metastatic thoracic ESCC lesion in Wistar rats, transplantable and tumor-forming in nude mice. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.

T8335 2XSB Cells

T8335 2XSB Cells are a sporadic human MPNST cell line expressing S100&beta;, Sox10, and nestin, with intact NF1 allele and functional PRC2, invasive and tumorigenic in immunodeficient mice. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.

T8337 RCC BA85#21 Cells

T8337 RCC BA85#21 Cells are an immortalized, non-aggressive, non-metastatic human clear cell renal cell carcinoma cell line from a 62-year-old male, STR-profiled, with a ~72 hr doubling time. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.

<h2>What Are Proteases?</h2>
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Proteases (also called proteinases or peptidases) catalyze the hydrolysis of peptide bonds in proteins and polypeptides. They are classified by their catalytic mechanism into serine proteases (Proteinase K, trypsin, chymotrypsin, subtilisin), cysteine proteases (papain, calpain), aspartic proteases (pepsin), metalloproteases (thermolysin, carboxypeptidase A), and threonine proteases. In research applications, proteases serve roles that span nucleic acid purification, proteomics sample preparation, cell culture maintenance, recombinant protein processing, and structural biology. Trypsin cleaves at the carboxyl side of lysine and arginine residues and generates peptides in the 500-3,000 Da mass range ideal for LC-MS/MS peptide identification. Select a protease based on substrate specificity (broad-spectrum vs. site-specific), compatibility with your lysis or reaction buffer, and the need for downstream inactivation.
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<h2 dir="ltr">What you will find:</h2>
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<a href="https://mbpinc.net/reagents/molecular-biology/enzymes-and-inhibitors/proteases/proteinase-k" title="Proteinase K:">Proteinase K:</a> A robust serine protease (available in lyophilized or liquid formats) designed for rapid protein digestion and the inactivation of nucleases during sensitive nucleic acid isolation.
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Aprotinin, A high-purity competitive inhibitor of serine proteases, is essential for preventing unwanted proteolytic activity during delicate protein purification and extraction steps.
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Protease Inhibitor Cocktail, A pre-formulated, multi-component blend engineered to provide immediate, broad-range protection against a variety of endogenous proteases in cellular lysates.
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TEV Protease, A specialized endopeptidase with ultra-fine specificity, is utilized for the precise, site-directed cleavage of affinity tags from recombinant fusion proteins.
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<h2>How to Choose Proteases</h2>
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Nucleic acid purification
Proteinase K is the first choice for nucleic acid extraction because it is a broad-spectrum serine endopeptidase that digests nucleases (DNases, RNases), histones, and structural proteins in the presence of SDS, urea, EDTA, and most detergents. It is active from pH 4.0 to 12.5 with optimal activity at pH 8.0, and at temperatures from 20 to 60 degrees C. Inactivation at 95 degrees C for 5 minutes or by proteinase K inhibitors (PMSF) is required before downstream enzymatic steps.
Proteomics and mass spectrometry
Trypsin (sequencing grade) is the industry standard for bottom-up proteomics: it generates predictable lysine/arginine-terminated peptides of 6-25 amino acids, well-suited for LC-MS/MS analysis on Orbitrap and Q-TOF platforms. Chymotrypsin (cleaves after Phe, Tyr, Trp, Leu) and Glu-C/V8 protease (cleaves after Glu in phosphate buffer, or Glu+Asp in bicarbonate) are used for orthogonal digestions to improve protein sequence coverage. Lys-C protease (cleaves after Lys only) is stable in 8 M urea and is used for denatured or hard-to-digest proteins.
Cell culture dissociation
Trypsin at 0.25% (w/v) with 0.53 mM EDTA is the standard reagent for detaching adherent mammalian cells from culture surfaces. Recombinant trypsin formulations avoid the lot variability and animal-derived pathogen concerns of porcine pancreatic trypsin. Collagenase (types I, II, III, IV) is used for tissue dissociation, particularly for primary cell isolation from solid tissues, including liver, pancreas, breast, and adipose.
Recombinant protein processing
Site-specific proteases are used to remove N-terminal affinity tags (His6, GST, MBP) from purified recombinant proteins without leaving extra residues. Thrombin cleaves after Arg in the sequence LVPR/GS; Factor Xa cleaves after Arg in IE/DGR; TEV protease cleaves between Gln and Ser in ENLYFQ/S. TEV is the most commonly used because of its stringent 7-amino-acid recognition sequence, which minimizes off-target cleavage.
Buffer compatibility
Confirm protease compatibility with your lysis buffer before use. Proteinase K retains activity in 1% SDS, 4 M urea, 1% Triton X-100, and 10 mM EDTA. Trypsin is inhibited by EDTA (chelates the Ca2+ that stabilizes trypsin structure) and by soybean trypsin inhibitor (SBTI). Chymotrypsin is inhibited by PMSF (an irreversible serine protease inhibitor). Factor Xa is inhibited by benzamidine.
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<h2>Specifications Context</h2>
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Trypsin activity for proteomics is expressed in TAME units (N-alpha-p-tosyl-L-arginine methyl ester esterase units); 1 TAME unit hydrolyzes 1 micromol of TAME per minute at 25 degrees C, pH 8.1. Sequencing-grade trypsin is treated with TPCK (tosylphenylalanyl chloromethyl ketone) to irreversibly inactivate contaminating chymotrypsin activity, which would otherwise generate non-tryptic peptides that complicate database searches. Proteinase K activity is expressed as mAnson units per mL or mg, where 1 mAnson unit releases 1 micromol of tyrosine equivalents per minute from hemoglobin at pH 7.5 and 35.5 degrees C. Single-pot, solid-phase-enhanced sample preparation (SP3) using magnetic bead-bound proteases is increasingly adopted in proteomics to reduce sample loss and processing time for low-input samples.&nbsp;
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Secure your sample stability&mdash;<a href="https://mbpinc.net/contact-us">contact the MBP team</a> today to request a quote for our professional-grade proteases.

Proteases

MSDS

TEV Protease

Unit Quantity

Application

Concentration

Shipping Condition

THJ11T Cells

T8251 THJ11T Cells are a Stage IV human anaplastic thyroid carcinoma cell line from a 45-year-old male donor, demonstrated to be invasive and tumorigenic in mice. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.

THJ16T Cells

T8252 THJ16T Cells are Stage IV human anaplastic thyroid cancer cells expressing p53 R273H and PIK3CA E545K oncogene mutations, isolated from an adult female patient. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.

THJ21T Cells

THJ21T Cells (T8253) by MBP are derived from anaplastic thyroid carcinoma stage IV and express p53 R280T, BRAF V600E, and NF1 D176E. Adherent epithelial-like, thyroid. PDT 43h. BSL II. 1&times;106 cells / 1.0 mL, frozen. MTA required.

THJ29T Cells

THJ29T Cells (T8254) by MBP are derived from anaplastic thyroid carcinoma stage IV and express p53 Q104X and ALK S426T. Tumorigenic in athymic nude mice. PDT 35h. BSL II. 1&times;106 cells / 1.0 mL, frozen. MTA required.