Zymo Reagents

<h2>What are microbial and environmental DNA isolation kits?</h2>
&nbsp;
Microbial and environmental DNA isolation kits purify DNA from soil, sediment, freshwater, marine water, feces, and other complex environmental matrices using lysis and purification chemistry designed to remove humic acids, metal compounds, and other inhibitors that interfere with PCR and sequencing, supporting 16S/18S rRNA amplicon profiling and shotgun metagenomic sequencing. Related entities include "kitome" and "splashome" contamination controls, alpha diversity and microbial community composition analysis, and compatibility with both short-read (Illumina) and long-read (Oxford Nanopore) sequencing platforms. Choose a kit validated for your specific sample matrix (soil, sediment, water, or feces), since benchmarking studies consistently show kit performance varies by matrix rather than one kit being universally best.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Spin-column fungal and bacterial kits for rapid DNA purification using rapid silica-membrane technology&nbsp;</li>
<li dir="ltr" role="presentation">Parallel extraction of soil, fecal, and environmental samples using high-throughput 96-well microplates&nbsp;</li>
<li dir="ltr" role="presentation">Automated&nbsp; purification of complex microbial and environmental specimens using Magnetic bead isolation systems</li>
<li dir="ltr" role="presentation">Scalable microprep and midiprep columns tailored for concentrated low-volume elution demands and diverse sample inputs</li>
</ul>
&nbsp;
<h2>How to choose a microbial and environmental DNA isolation kit</h2>
&nbsp;
Matrix-specific kit performance
Benchmarking studies comparing multiple commercial kits across sediment, water, soil, oyster gut microbiome, and mammalian feces have found that the best-performing kit varies by matrix - kits ranked highly for sediment did not necessarily perform best for water or host-associated samples. Rather than assuming a single "universal" kit, match your kit choice to published benchmarking results for your primary sample type where available.
Inhibitor removal for downstream PCR and sequencing
Environmental samples, especially sediments and soils, contain organic and metal compounds that inhibit PCR amplification if not removed during extraction; kits designed for these matrices integrate inhibitor-removal steps as part of the standard protocol. If your samples have historically produced low PCR amplification efficiency with general-purpose kits, an environmental-matrix-specific kit with built-in inhibitor removal is the first thing to evaluate.
Short-read vs. long-read sequencing compatibility
For long-read metagenomic sequencing, DNA fragment length and integrity from the extraction kit directly affect achievable read lengths, though longer extracted fragments don't always translate consistently into longer sequencing reads - kit choice affects both fragment length and downstream classification rate and reproducibility. If long-read metagenomics is your goal, prioritize kits with published long-read benchmarking data over kits validated only for short-read amplicon work.
Contamination control (kitome/splashome)
Kit-derived contamination ("kitome") and cross-sample contamination during processing ("splashome") can affect microbial community composition results, particularly for low-biomass samples; benchmarking studies evaluate kits specifically for these contamination sources alongside yield and diversity metrics. For low-biomass environmental samples, prioritize kits with documented low kitome contamination in published comparisons.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for microbial and environmental DNA isolation are matrix-specific validation (soil vs. sediment vs. water vs. feces), built-in inhibitor removal for PCR-ready output, and fragment length/integrity for long-read sequencing compatibility where relevant. Since benchmarking studies repeatedly show no single kit performs best across all matrices, identifying your primary sample type before selecting a kit is the most impactful decision. Labs running metagenomic surveys across multiple matrices (e.g., soil and water from the same field site) often need separate matrix-optimized kits rather than a single universal kit. The parent <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits hub</a>&nbsp;links to related sample-type categories, including&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/insect">insect genomic DNA kits</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/plant">plant genomic DNA kits</a>&nbsp;for organism-associated environmental work, and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/high-molecular-weight-dna">high molecular weight DNA kits</a>&nbsp;for long-read metagenomics requiring larger fragment sizes. For cell-free DNA in environmental water samples (eDNA detection), see&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/cell-free-dna">cell-free DNA kits</a>.&nbsp;
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Microbial & Environmental DNA Isolation

DataSheet

MSDS

Protocol

Quick-DNA™ Fungal/Bacterial Microprep Kit (50 Preps.)

Quick-DNA™ Fungal/Bacterial Microprep Kit (D6007) by Zymo Research — 50 preps, ≤15 min. BashingBead mechanical lysis for fungi, Gram+/- bacteria, algae, and protozoa. Yields ≤5 µg DNA at 25–35 kb fragments; elution ≥10 µl; A260/A280 ≥1.8.

Applicable For

Elution Volume

Equipment

Processing Time

Processing Volume

Purity

Sample Source

Size Range

Type

Yield

Manufacturer

Shipping Condition

Storage Condition

Quick-DNA™ Fungal/Bacterial Midiprep Kit (25 Preps.)

D6105 by Zymo Research isolates up to 125 µg of genomic DNA from 250–500 mg of tough-to-lyse fungi or bacteria in as little as 20 minutes using mechanical bead-beating lysis. The Quick-DNA™ Fungal/Bacterial Midiprep Kit (25 preps) produces high-molecular-weight DNA (25–35 kb, A260/A280 &gt;1.8) suitable for PCR, WGS, RFLP, and Southern blotting. All reagents store at room temperature.

Optimized Lysis Protocols

Quick-DNA™ Fungal/Bacterial Miniprep Kit (50 Preps.)

The Quick-DNA™ Fungal/Bacterial Miniprep Kit (D6005) by Zymo Research extracts up to 25 µg of total genomic DNA from fungi, bacteria, spores, algae, and protozoa in ≤15 minutes using BashingBead mechanical lysis. Typical DNA fragment size 25–35 kb; purity A260/A280 ≥1.8. 50 preps per kit; eluate is compatible with qPCR, NGS, and arrays.

<h2>What Are Magnetic Bead HMW DNA Extraction Kits?</h2>
&nbsp;
A magnetic bead HMW DNA extraction kit uses Solid Phase Reversible Immobilization (SPRI)-type magnetic beads to bind genomic DNA released by gentle lysis, with a magnetic separator handling wash and elution steps in place of centrifugation, columns, or vacuum manifolds, producing fragment sizes in the 50-150 kb range depending on how beads are resuspended during elution. Related entities include PacBio Nanobind-type bead chemistry, KingFisher-compatible automated magnetic particle processors, A260/280 and A260/230 purity thresholds above 1.8, and downstream long-read sequencing library preparation for PacBio and Oxford Nanopore platforms. Choose magnetic bead HMW kits when fragment sizes in the 50-150 kb range meet your long-read sequencing needs, and you want to avoid centrifugation-based shearing.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>Low-shear high molecular weight DNA isolation solutions using advanced paramagnetic bead technology, including the Magbio HighPrep HMW DNA Kit.</li>
<li>High-throughput magnetic bead-based extraction systems for simultaneous recovery of intact genomic DNA, including the Quick-DNA&trade; HMW Kit.</li>
<li>Workflow-friendly HMW DNA purification kits designed to preserve long DNA fragments for long-read sequencing applications.</li>
<li>Solutions compatible with manual and automated sample processing for efficient genomic DNA recovery.</li>
<li>HMW DNA extraction products suitable for genome assembly, structural variant analysis, and other advanced genomics workflows.</li>
</ul>
&nbsp;
<h2>How to Choose a Magnetic Bead HMW DNA Kit</h2>
&nbsp;
Bead Resuspension Method and Fragment Size Tradeoff
Fragment size from magnetic bead HMW kits depends on how beads are resuspended after washing: gentle tube tapping typically yields fragments in the 50-150 kb range, while vortexing produces somewhat smaller fragments (around 40-100 kb) but may improve recovery consistency. If your sequencing application benefits from the largest possible fragments (e.g., resolving large structural variants), prioritize tapping-based resuspension protocols even if yield is marginally lower.
Sample Input and Expected Yield
Magnetic bead HMW kits for blood, cells, and tissue typically specify inputs such as 200 ul of fresh/frozen whole blood, 1 million cells, or up to 8 mg of tissue, with a minimum yield around 3 ug of HMW gDNA&mdash;matching the input requirements of PacBio library preparation (around 1 ug above 30 kb) and Oxford Nanopore ligation kits (100 ng to 1.5 ug). Confirm your sample type matches a documented protocol within the kit family rather than assuming a single universal protocol.
No Centrifuge, Column, or Vacuum Required
Unlike silica spin-column or 96-well plate HMW approaches, magnetic bead HMW kits eliminate centrifugation, columns, and vacuum manifolds entirely, relying only on a magnetic separator for all wash and elution steps. This simplifies equipment requirements for labs without access to specialized centrifuge rotors, though a magnetic stand or automated magnetic particle processor is still required.
Purity Ratios and RNA Removal
A260/280 and A260/230 ratios greater than 1.8 are generally preferred for HMW DNA from magnetic bead kits, with near-complete RNA removal reported as a feature of bead-based HMW chemistry. Ratios below this threshold can indicate protein or salt contamination requiring troubleshooting before proceeding to expensive long-read library preparation.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The defining specifications for magnetic bead HMW DNA extraction are the fragment size achievable via the bead resuspension method chosen, sample-specific input requirements relative to the kit's documented minimum yield, and purity ratios above 1.8 for both A260/280 and A260/230. A kit yielding around 3 ug of DNA in the 50-150 kb range from 200 ul whole blood, 1 million cells, or up to 8 mg tissue meets standard PacBio and Nanopore library preparation inputs without centrifugation.&nbsp;
&nbsp;
<section class="mbp-contact-cta">
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Mag Beads

Quick-DNA™ HMW kit (96 Preps.)

The D6060 by Zymo Research is a 96-prep magnetic bead-based HMW DNA extraction kit recovering fragments up to 150 kb (typical 20–200 kb) at A260/A230 ≥ 1.8, with 10 µg binding capacity per 50 µl beads. Compatible with cells, tissue, blood, saliva, and buccal swabs; optimized for Oxford Nanopore and PacBio SMRT long-read sequencing. Made in the USA.

DNA Purity

DNA Size

DNA Yield

Sample Types

<h2>What Are Magnetic Bead Genomic DNA Kits for Blood and Tissue?</h2>
&nbsp;
Magnetic bead genomic DNA kits for blood and tissue use paramagnetic beads to capture genomic DNA released during sample lysis. Following DNA binding, a magnetic separator immobilizes the beads, and contaminants are removed by wash steps before purified DNA is eluted. Unlike spin-column workflows, magnetic bead protocols eliminate centrifugation and simplify automation, making them well-suited to high-throughput laboratories and automated liquid-handling systems.
Magnetic bead extraction is often selected when automation, reduced hands-on processing, or compatibility with high-throughput workflows is a priority. Specialized high-molecular-weight (HMW) magnetic bead chemistries are also available for applications requiring longer DNA fragments for advanced sequencing workflows.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>Quick-DNA&trade; Magbead Plus Kit: Magnetic bead-based genomic DNA purification for blood, tissue, cultured cells, and other biological samples.</li>
<li>Automation-Compatible Workflows: Designed for seamless integration with manual and automated magnetic separation platforms.</li>
<li>High-Yield DNA Recovery: Optimized to produce purified genomic DNA suitable for PCR, qPCR, genotyping, sequencing, and other downstream molecular biology applications.</li>
<li>Streamlined Sample Processing: Eliminates centrifugation steps through magnetic bead technology, simplifying genomic DNA extraction workflows.</li>
</ul>
<h2>&nbsp;</h2>
<h2>How to Choose a Magnetic Bead Genomic DNA Kit for Blood and Tissue</h2>
&nbsp;
Sample Input and Processing Time
Most magnetic bead kits are designed to process approximately 100-400 &micro;L of whole blood or buffy coat per preparation. Tissue and cultured cell inputs vary by kit but generally support small tissue samples and several million cells per extraction. Manual workflows can often be completed in about 20 minutes because centrifugation steps are eliminated.
Yield and Purity Requirements
Magnetic bead extraction kits are capable of producing high-quality genomic DNA suitable for PCR, qPCR, genotyping, sequencing, Southern blotting, and related molecular biology applications. DNA purity is commonly comparable to silica-based purification methods, making these kits suitable for most downstream workflows.
Automation Compatibility
One of the primary advantages of magnetic bead chemistry is its compatibility with automated liquid handling platforms. Because DNA capture and washing occur through magnetic separation rather than centrifugation, these workflows can be readily adapted to 96-well and higher-throughput automated formats.
High-Molecular-Weight DNA Applications
Some magnetic bead systems are specifically optimized for high-molecular-weight genomic DNA recovery. These workflows can preserve larger DNA fragments required for long-read sequencing, linked-read assembly, structural variation analysis, and other applications where DNA integrity is critical.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Magnetic bead genomic DNA extraction kits are differentiated by their centrifuge-free workflow, automation compatibility, and flexibility across blood, buffy coat, tissue, and cultured cell samples. The magnetic separation process reduces manual handling steps while supporting consistent DNA recovery and scalable throughput.&nbsp;Many laboratories adopt magnetic bead workflows when transitioning from manual extraction methods to automated sample preparation systems. Compared with traditional spin-column methods, magnetic bead purification simplifies integration with robotic liquid handlers and high-throughput extraction platforms while maintaining DNA quality suitable for PCR, qPCR, sequencing, and genotyping applications.&nbsp;When selecting a kit, evaluate sample input requirements, automation compatibility, DNA purity specifications, and whether high-molecular-weight DNA recovery is required for downstream applications. Most kit reagents are stored according to manufacturer recommendations, and lot-specific Certificates of Analysis (CoAs) are available upon request.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP</a> to get high-quality mag beads for blood/tissue genomic DNA extraction workflows to scale up your laboratory throughput.

D4081-Data Sheet

D4081-1-MSDS

D4081-2-MSDS

D4081-3-MSDS

D4081-Protocol

Quick-DNA™ Magbead Plus Kit

D4081 by Zymo Research is the Quick-DNA™ Magbead Plus Kit, a magnetic bead-based platform for total DNA extraction (genomic, mitochondrial, and viral) from biological fluids, cells, and solid tissues in a 96-well format, with Proteinase K included and automation scripts available for KingFisher, Tecan, Hamilton, and Opentrons OT-2. The kit recovers DNA fragments up to 150 kb and processes 96 samples in approximately 60 minutes.

Workflow

Quick-DNA™ Magbead Plus Kit (96 Preps.)

Quick-DNA™ Magbead Plus Kit (4 x 96 Preps.)

Solid Tissue Digestion Buffer II (22 ml)

Solid Tissue Digestion Buffer II (6 ml)

BioFluid & Solid Tissue Buffer (100 ml)

BioFluid & Solid Tissue Buffer (25 ml)

<h2>What Are Spin-Column Genomic DNA Kits for Blood and Tissue?</h2>
&nbsp;
A spin-column genomic DNA kit for blood and tissue combines a proteinase K-based direct lysis step with a silica-membrane spin column to purify genomic, mitochondrial, or viral DNA from animal blood, tissue, and cultured cells without phenol, chloroform, or alcohol precipitation. Related entities include direct-lysis protocols, dried blood spot supplementary workflows, TissueLyser mechanical disruption, and downstream PCR, genotyping, and sequencing library preparation.&nbsp;Use spin-column formats for individual or small-batch genomic DNA extraction from blood, cells, or tissue using a standard microcentrifuge.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li>Efficient isolation of high-quality genomic DNA from blood, saliva, tissues, and other biological samples using rapid spin-column purification.&nbsp;</li>
<li>Maximize DNA recovery with optimized lysis and binding chemistry while minimizing inhibitor, protein, and contaminant interference</li>
<li>Midiprep, microprep, and miniprep formats available to support varied sample input volumes&nbsp;</li>
<li>User-friendly workflows and streamlined protocols with fast processing times for efficient genomic DNA extraction&nbsp;</li>
</ul>
&nbsp;
<h2>How to Choose a Spin-Column Blood/Tissue Genomic DNA Kit</h2>
&nbsp;
Lysis Approach by Sample Type
Whole blood, buffy coat, buccal cells, and cultured cells generally undergo direct proteinase K lysis without mechanical disruption. In contrast, fibrous or fatty tissues such as muscle, liver, or skin often require mechanical homogenization prior to the lysis step to ensure complete cell disruption.
Anticoagulant and Blood Sample Handling
These kits typically support fresh or frozen blood collected in EDTA, citrate, or heparin. Dried blood spots generally require supplementary protocols and additional equipment beyond standard spin-column workflows.
Column Elution Chemistry
Elution is typically performed using Tris-based buffer or nuclease-free water, with volumes ranging from approximately 50&ndash;200 &micro;L depending on kit design and sample type. Water elution may be preferred for EDTA-sensitive downstream applications.
Scaling Beyond a Single Column
For higher throughput workflows, 96-well plate formats using the same silica-membrane chemistry enable simultaneous processing of multiple samples per centrifuge run. Spin-column formats remain best suited for method development and low-to-moderate sample volumes.
&nbsp;
<h2>Specifications Context:</h2>
&nbsp;
The most important parameters for spin-column blood/tissue genomic DNA kits are sample-type compatibility and elution buffer composition, as these determine whether additional pretreatment is required and whether the eluate is directly compatible with downstream workflows. A typical 200 &micro;L whole blood input yields low-microgram quantities of genomic DNA in an elution volume of approximately 50&ndash;200 &micro;L.
&nbsp;
Use MBP's <a href="mbpinc.net/quickorder">Quick Order tool</a> for bulk pricing or <a href="mbpinc.net/contact-us">contact the specialist team</a> to confirm pretreatment requirements for challenging tissue types.

Spin-column

D4074-Data Sheet

D4074-MSDS

D4074-Protocol

Quick-DNA™ Microprep Plus Kit (50 Preps.)

D4074 by Zymo Research — Quick-DNA™ Microprep Plus Kit (50 preps) for high molecular weight genomic DNA extraction (&gt; 50 kb) from tissues, blood, saliva, cell cultures, and biological fluids. 20-minute protocol; elution ≤ 10 µl; binding capacity up to 5 µg. Ready for qPCR, NGS, and microarray.

D4075-Data Sheet

D4075 - MSDS

D4075-Protocol

Quick-DNA™ Midiprep Plus Kit (25 Preps.)

D4075 Quick-DNA Midiprep Plus Kit by Zymo Research — 25 preps; &gt;50 kb genomic DNA; 125 &micro;g column capacity; &le;200 &micro;l elution; tissues, blood, cells, FFPE, hair, and bone.

D4068-Data Sheet

D4068/D4068T - MSDS

D4069 - MSDS

D4068-Quick Protocol

D4068-Protocol

Quick-DNA™ Miniprep Plus Kit

D4068 by Zymo Research — Quick-DNA™ Miniprep Plus Kit with Proteinase K; 20-minute protocol; ≥35 µL elution; 25 µg capacity; >50 kb recovery; A260/A230 ≥ 2.0; for tissue, blood, saliva, and biological fluids. 10, 50, or 200 preps.

BioFluid & Cell Buffer (Red) (6 ml)

BioFluid & Cell Buffer (Red) (12 ml)

BioFluid & Cell Buffer (Red) (25 ml)

BioFluid & Cell Buffer (Red) (45 ml)

Solid Tissue Buffer (Blue) (10 ml)

Solid Tissue Buffer (Blue) (22 ml)

Solid Tissue Buffer (Blue) (6 ml)

Genomic Binding Buffer (25 ml)

Genomic Binding Buffer (45 ml)

Genomic Binding Buffer (85 ml)

Quick-DNA™ Miniprep Plus Kit (10 Preps.)

Quick-DNA™ Miniprep Plus Kit (200 Preps.)

Quick-DNA™ Miniprep Plus Kit (50 Preps.)

<h2>What are 96-well plate plant genomic DNA kits?</h2>
&nbsp;
A 96-well plate plant genomic DNA kit replaces individual spin columns with a silica binding plate, processed by a centrifuge with a 96-well rotor carrier, to purify genomic DNA from up to 96 plant tissue samples per run using CTAB- or SDS-based lysis chemistry adapted to a plate format. Related entities include plate-based pre-filtration steps for polysaccharide removal, high-throughput genotyping and marker-assisted breeding workflows, and downstream PCR and SNP genotyping applications. Choose the 96-well plate format for plant genomic DNA when weekly sample numbers across multiple individuals or accessions justify batch processing on a plate-compatible centrifuge.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>Quick-DNA&trade; Plant/Seed 96 Kit for rapid vacuum manifold processing using silica-membrane 96-well plant DNA plates</li>
<li>Silica-membrane 96-well plates deliver consistent genomic DNA recovery for downstream PCR and genotyping</li>
<li>Optimized plant and seed DNA extraction workflow supports efficient large-scale breeding and research projects</li>
</ul>
&nbsp;
<h2>How to choose a 96-well plate plant genomic DNA kit</h2>
&nbsp;
Lysis chemistry consistency across the plate
Because all 96 wells are processed together, lysis chemistry (CTAB vs. SDS) needs to suit every sample on the plate; mixing species with very different polysaccharide or polyphenol content on one plate can mean some wells underperform if the chemistry is not matched to the most demanding sample type. Validate lysis chemistry on individual spin columns for a new species before committing to plate-scale runs.
Centrifuge rotor and pre-filtration in plate format
96-well plant DNA kits require a centrifuge with a 96-well rotor carrier, and pre-filtration to remove polysaccharides and debris is typically built into the plate workflow using a pre-filter plate stacked above the binding plate rather than a separate Maxi column. Confirm your centrifuge accepts the stacked-plate configuration the kit specifies.
Tissue input per well and plate layout
Each well in a 96-well plant DNA kit typically accepts a similar tissue mass to a single spin-column preparation, under 100 mg fresh weight, so plate layouts should account for tissue collection and loading time per well alongside the extraction itself. For breeding programs processing many individuals per accession, planning plate layout by population or family can simplify downstream genotyping data handling.
Throughput vs. species diversity
A single 96-well plant DNA plate is most efficient when processing many samples of the same or closely related species with similar metabolite profiles; highly diverse sample sets may be better split across plates by lysis chemistry requirement. Estimate species diversity within a typical batch before committing to one chemistry for an entire plate run.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The defining specifications for a 96-well plate plant genomic DNA kit are lysis chemistry suitability across all samples on the plate, centrifuge rotor and stacked pre-filter plate compatibility, and per-well tissue input consistent with spin-column equivalents. A kit processing under 100 mg fresh tissue per well with matched CTAB or SDS chemistry and built-in pre-filtration scales the same yield range, roughly 1&ndash;30 micrograms, across 96 samples per run. Breeding and genotyping programs often validate lysis chemistry using <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/plant/spin-column">plant spin-column kits</a> for a new species or accession set before committing to plate-scale batches with this format.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get high-throughput extraction using an optimized 96-well system.

96-well plate

MSDS (EU)

MSDS (US)

User Manual

Quick-DNA™ Plant/Seed 96 Kit (2 x 96 Preps.)

D6021 by Zymo Research — Quick-DNA™ Plant/Seed 96 Kit; 2 × 96 preps; processes stems, roots, leaves, fruits, and seeds (≤80 mg input); BashingBead™ lysis; ≥50 µL elution; inhibitor-free DNA for qPCR, NGS, and arrays.

Equipment	

Sample Storage

<h2>What are plant spin-column genomic DNA kits?</h2>
&nbsp;
A plant spin-column genomic DNA kit pairs a CTAB- or SDS-based lysis buffer with a single silica-membrane spin column to purify genomic DNA from one plant tissue sample at a time, on a standard microcentrifuge with a swing-out rotor for larger lysate volumes. Related entities include pre-filter homogenization columns, high-salt CTAB buffers, PVP for polyphenol binding, and downstream PCR, restriction digestion, and RAPD marker applications. Choose the spin-column format for individual plant samples or method development on a new species before scaling to a 96-well plate.
&nbsp;
<h2>What you will find</h2>
&nbsp;
<ul>
<li>Rapid benchtop processing using silica-membrane spin-column plant DNA kits</li>
<li>Plant genomic DNA columns optimized with polysaccharide-clearing properties for p-starchy crop tissues</li>
<li>Polyphenol-resistant plant extraction spin-columns with polysaccharide-clearing features</li>
<li>Meeting low-volume elution demands using micro-elution plant DNA purification columns</li>
</ul>
&nbsp;
<h2>How to choose a plant spin-column genomic DNA kit</h2>
&nbsp;
Matching lysis chemistry to tissue type
Spin-column kits in this category are typically offered with CTAB-based lysis (for high-polysaccharide or high-polyphenol species) or SDS-based lysis (for lower-metabolite tissue such as young leaves of common model species). Identify your species' secondary metabolite profile before choosing, since the wrong lysis chemistry can result in low yield or DNA that inhibits downstream PCR.
Sample input and pre-filtration
Plant spin-column kits typically process under 100 mg of fresh-weight tissue or under 20 mg of dried tissue per column, often requiring a pre-filtration step through a separate homogenization column to remove debris and precipitated polysaccharides before loading onto the DNA-binding column. Skipping this step is a common cause of column clogging with fibrous tissue.
Centrifuge requirements for larger lysate volumes
Some plant CTAB protocols generate larger lysate volumes than animal blood/tissue protocols and may call for centrifugation at 3,000&ndash;5,000 &times; g using a swing-out rotor for Maxi-format pre-filter columns, in addition to standard microcentrifuge steps for the final DNA-binding column. Confirm your centrifuge has a swing-out rotor option if your protocol specifies Maxi-format pre-filtration.
Scaling to batch processing
If your sample numbers grow beyond what individual columns handle efficiently per run, a 96-well plate version of plant genomic DNA chemistry processes multiple samples per centrifuge run; the spin-column format remains the right starting point for method development and lower-volume work.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The specs that most affect a plant spin-column kit choice are lysis buffer chemistry matched to tissue type, pre-filtration requirements, and the centrifuge rotor options needed for larger lysate volumes in CTAB protocols. A kit processing under 100 mg fresh tissue with appropriate CTAB or SDS chemistry and a pre-filtration step typically yields 1&ndash;30 &micro;g of DNA suitable for PCR and restriction analysis. Labs developing protocols for new or recalcitrant species often start with this spin-column format to validate lysis chemistry before moving to <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/plant/96-well-plate">plant 96-well plate kits</a> for routine batches. The parent <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/plant">plant genomic DNA extraction kits category</a> and broader <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits hub</a> cover related formats, and for animal-sample work, the <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/blood/tissue/spin-column">blood/tissue spin-column kits</a> use comparable column chemistry with a different lysis buffer.
&nbsp;
<a href="https://mbpinc.net/contact-us" style="text-decoration: underline;">Contact the expert team at MBP</a><a href="https://mbpinc.net/contact-us" style="text-decoration: underline;"> </a><a href="https://mbpinc.net/contact-us" style="text-decoration: underline;">Inc.</a> to get high-quality spin-column systems to streamline your plant research.

Quick-DNA™ Plant/Seed Miniprep Kit (50 Preps.)

D6020 by Zymo Research is the Quick-DNA™ Plant/Seed Miniprep Kit (50 preps). Isolates inhibitor-free DNA from leaves, stems, buds, flowers, fruit, and seeds using ZR BashingBead™ Lysis Tubes (2 mm). DNA yield ≤25 µg; elution ≥50 µl. For PCR, qPCR, and NGS from plant material.

<h2>What are 96-well plate insect genomic DNA kits?</h2>
&nbsp;
A 96-well plate insect genomic DNA kit combines bead-beating lysis using ultra-high-density bashing beads with silica-membrane spin plate technology to purify genomic, viral, or mitochondrial DNA from up to 96 fresh, frozen, or stored insect specimens per run, with the lysis-to-elution procedure designed to complete in minutes once plates are loaded. Related entities include high-density zirconium/ceramic bashing beads for mechanical disruption, compatibility with mammalian tissue, whole blood, and cultured cells in the same kit, and downstream PCR, array, and genotyping workflows. Choose the 96-well plate format for insect genomic DNA when processing large specimen batches (mosquitoes, flies, bees, ticks, lice) without dissecting each insect individually.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>Rapid vacuum manifold processing using silica-membrane 96-well insect DNA plates</li>
<li>Automated extraction systems using magnetic bead 96-well insect genomic DNA plates</li>
<li>Optimized extraction chemistry using 96-well insect and invertebrate purification kits</li>
<li>Contamination-free insect sample centrifugation using siliconized plate sealing mats</li>
</ul>
&nbsp;
<h2>How to choose a 96-well plate insect genomic DNA kit</h2>
&nbsp;
Bead-beating lysis built into the workflow
Unlike single-specimen protocols that may need a separate homogenization step, 96-well insect plate kits typically integrate bead beating with high-density bashing beads directly into the plate workflow, lysing whole specimens, including chitin-rich exoskeletons, without a separate disruption device beyond a plate shaker or bead mill. Confirm your lab has access to a plate-compatible bead mill or shaker if the kit specifies this step.
Specimen size and whole-body processing
96-well insect plate kits are documented for small to medium specimens such as mosquitoes, D. melanogaster, lice, ticks, and bees processed whole, without dissection. For larger insects, per-well specimen mass may need to be limited to fit standard well volumes; check the kit's specimen size guidance before loading a full plate of larger-bodied insects.
Throughput and processing time
A full 96-well insect DNA extraction can be completed in a matter of minutes for the bead-beating and binding steps combined, compared to hours for some CTAB-style batch protocols, making this format suitable for museum or field-collection-scale specimen processing. Plan plate loading and bead-mill cycling time into your overall throughput estimate, since the chemistry itself is fast, but specimen handling adds time.
Cost considerations at scale
Commercial 96-well silica plate kits for insects and tissue can run several dollars per sample at typical case pricing, while silica membrane filtration plates used in lower-cost protocols can bring per-sample reagent cost down significantly, though usually with added hands-on steps. For large-scale museomics or population genetics projects processing thousands of specimens, per-sample cost differences compound quickly and are worth comparing against&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/insect/mag-beads">insect mag bead kits</a>&nbsp;as an alternative.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The defining specs for a 96-well plate insect genomic DNA kit are whether bead-beating lysis is integrated into the plate workflow, specimen size compatibility for whole-body processing, and per-sample cost at scale for large specimen collections. A kit that lyses whole mosquitoes, flies, or ticks via bead beating and purifies via silica plate technology in minutes addresses both the chitin-lysis challenge and batch throughput in one workflow. Labs processing large specimen collections often compare this format against <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/insect/mag-beads">insect mag bead kits</a>, which avoid centrifugation entirely and may suit automated leg/wing-based sampling, while labs validating protocols on a new taxon typically start with&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/insect/spin-column">insect spin-column kits</a>. The parent&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/insect">insect genomic DNA extraction kits category</a>&nbsp;and broader&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits hub</a>&nbsp;link to related formats, including&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/plant/96-well-plate">96-well plate plant kits</a> for pest-research labs working across both sample types.&nbsp;
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Quick-DNA™ Tissue/Insect 96 Kit (2 x 96 Preps.)

The Quick-DNA™ Tissue/Insect 96 Kit (D6017) by Zymo Research extracts ~5 µg genomic DNA per well from insects (mosquitoes, ticks, bees, Drosophila) and solid tissues in a 96-well BashingBead lysis format. Fragment size up to 40 kb+; purity A260/A280 ≥1.8; eluate ≥25 µl; 2 × 96 preps. Co-recovers parasitic and viral DNA if present.

<h2>What are insect spin-column genomic DNA kits?</h2>
&nbsp;
An insect spin-column genomic DNA kit pairs proteinase K-based lysis with a single silica-membrane spin column to purify genomic, mitochondrial, or microbial DNA from one whole insect or dissected insect tissue sample at a time, processed on a standard microcentrifuge, with optional mechanical disruption (bead beating, grinding) for chitin-rich specimens before the lysis step. Related entities include COI barcode gene amplification, high molecular weight DNA output for long-read sequencing, and multi-organism kits validated for insects alongside blood, tissue, bacteria, and yeast. Choose the spin-column format for individual insect specimens - whether for one-off identification or method development before scaling to batch processing.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>Rapid benchtop processing using silica-membrane spin-column insect DNA kits</li>
<li>Flexible invertebrate matrix handling using advanced insect extraction spin-columns</li>
<li>High-purity downstream templates using inhibitor-free insect genomic DNA purification units</li>
</ul>
&nbsp;
<h2>How to choose an insect spin-column genomic DNA kit</h2>
&nbsp;
Matching the lysis approach to the specimen type
Soft-bodied insects, larvae, or dissected internal tissue generally lyse adequately with proteinase K digestion alone, similar to standard animal tissue protocols, while hard-bodied or larger whole insects with significant chitin content often need mechanical disruption (bead beating, grinding, or homogenization) before or alongside lysis. Identify your specimen's exoskeleton characteristics before assuming the base proteinase K protocol applies without pretreatment.
Whole specimen vs. tissue dissection
Processing a whole small insect (e.g., Drosophila) in a spin-column kit is convenient and commonly documented, while larger insects may benefit from dissecting leg or thoracic muscle tissue to reduce the chitin-to-soft-tissue ratio in the lysate, improving DNA purity per unit input. Choose based on specimen size and whether dissection time is acceptable for your throughput needs.
Single-organism vs. multi-organism kit chemistry
Some spin-column kits are validated across multiple organism types - blood, tissue, bacteria, yeast, and insects - within one kit chemistry, useful for labs handling diverse sample types without switching kits. Confirm the kit's documentation explicitly addresses insects (and ideally your specific taxon) rather than relying on a general "tissue" claim.
DNA quality for downstream barcoding vs. sequencing
For COI barcoding and standard PCR, moderate-integrity DNA from a standard proteinase K protocol is generally sufficient, while population genetics or genome assembly projects benefit from kits documented to produce high molecular weight DNA (peak sizes around 50 kb or higher). Match your kit choice to the downstream application's DNA integrity requirements rather than defaulting to the highest-spec kit for routine barcoding.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The specs that most affect an insect spin-column kit choice are whether mechanical disruption is documented for your specimen's exoskeleton type, whether the kit chemistry is validated for insects specifically or only generically for "tissue," and the expected DNA fragment size relative to your downstream application. A kit suitable for both COI barcoding and population genetics, with peak fragment sizes around 50 kb or higher, covers a broad range of insect molecular work from a single specimen.&nbsp;The parent&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/insect">insect genomic DNA extraction kits category</a>&nbsp;and broader&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits hub</a>&nbsp;link to related formats, and for plant-sample work in pest-related research,&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/plant/spin-column">plant spin-column kits</a> use comparable column chemistry with different lysis buffers.&nbsp;
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Spin-Column

Quick-DNA™ Tissue/Insect Microprep Kit (50 Preps.)

D6015 by Zymo Research isolates genomic DNA from 1–10 mg of insects (mosquitoes, Drosophila, ticks, bees) or mammalian tissues in as little as 15 minutes using BashingBead mechanical lysis. The Quick-DNA™ Tissue/Insect Microprep Kit (50 preps) yields 1–5 µg/mg from insects and elutes into 10–20 µL (A260/A280 &gt;1.8), recovering fragments up to ≥40 kb. Room-temperature storage.

United States

Texas

mbpinc