Zymo Reagents

<h2>What are Magnetic Bead DNA/RNA Co-Extraction Kits for Cells, Tissue, and Blood?</h2>
&nbsp;
Magnetic bead DNA/RNA co-extraction kits for cells, tissue, and blood simultaneously isolate genomic DNA and total RNA, including small RNAs &gt;=17 nt from mammalian cell pellets (up to 5 &times; 10^6), solid tissue biopsies (up to 30 mg), and whole blood (up to 50 ul) in 96-well format using paramagnetic silica-coated beads on automation platforms. The Zymo Quick-DNA/RNA MagBead Kit includes DNA/RNA Shield for sample lysis and stabilization, Proteinase K for tissue digestion, and DNase I for on-bead gDNA removal from the RNA fraction, producing both analytes in separate eluates from the same input without any centrifugation during the purification phase. Choose the magnetic bead format for DNA/RNA co-extraction from cells, tissue, and blood when processing 48+ samples per session, when running gene therapy tissue panels across 10+ organ types, or when automation infrastructure eliminates the bottleneck of manual spin-column switching for large sample sets.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
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<li>Quick-DNA/RNA&trade; MagBead Kit&nbsp;for simultaneous isolation of total DNA and RNA from cells, tissues, and blood.</li>
<li>Magnetic particle&ndash;based purification for efficient nucleic acid recovery.</li>
<li>High-throughput workflow suitable for processing multiple samples.</li>
<li>Automation-ready protocol for streamlined laboratory workflows.</li>
<li>Purified DNA and RNA are compatible with downstream molecular biology applications.</li>
</ul>
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&nbsp;
<h2>How to Choose a Magnetic Bead DNA/RNA Co-Extraction Kit for Cells, Tissue, and Blood</h2>
&nbsp;
Automation Platform Compatibility
Confirm instrument scripts are available before kit purchase. The Zymo Quick-DNA/RNA MagBead Kit is validated on Tecan Fluent, Hamilton STAR, Beckman Coulter Biomek, and KingFisher platforms. For other instruments, consult MBP's technical team or contact Zymo Research automation support for custom script development timelines and requirements.
DNase I Inclusion and Timing
The Quick-DNA/RNA MagBead Kit includes DNase I for automated on-bead treatment, which integrates into the platform workflow script without additional liquid-handling steps. For spin-column co-extraction kits, DNase I treatment adds a separate 15-minute off-robot incubation step; the bead format eliminates this, reducing total protocol time for RNA-seq-grade RNA in 96-well batches.
Tissue Homogenization Before Bead Extraction
Solid tissue requires homogenization before adding lysate to the bead extraction plate. Tissue homogenization options: (a) Proteinase K digestion in DNA/RNA Shield at 55 degrees C for 30-60 minutes (soft tissues); (b) bead-mill homogenization using a 96-well bead mill with 2.8 mm ceramic beads (fibrous tissues); (c) MagBead Lysis Tubes for direct mechanical lysis in individual tubes before transfer to the 96-well plate. Confirm the homogenization approach is compatible with the platform's liquid transfer steps.
Sample Stabilization With DNA/RNA Shield
DNA/RNA Shield added at tissue collection stabilizes both DNA and RNA at room temperature for up to 30 days, enabling same-day or next-day extraction without cold-chain handling. This is particularly important for gene therapy biodistribution studies where multiple tissue types are collected from the same animal at necropsy and must maintain RNA integrity through shipping or batch processing delays.
Small RNA Retention
Silica-bead binding in the Quick-DNA/RNA MagBead Kit retains the full RNA size spectrum, including miRNAs &gt;=17 nt. For cancer multi-omics studies requiring paired DNA sequencing, RNA-seq, and small RNA-seq from the same tumour biopsy, this single kit delivers all three analyte types from one input without additional small RNA-specific extraction steps.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The Zymo Quick-DNA/RNA MagBead Kit yields high-quality gDNA and total RNA (including small RNAs &gt;=17 nt) from any biological sample, with both analytes ready for NGS, RT-qPCR, and microarray. A 2024 study validated simultaneous DNA and RNA extraction for AAV gene therapy tissue panels - adrenal gland, brain, gonads, heart, kidney, liver, lung, skeletal muscle, spinal cord, spleen- using automated co-extraction on a Beckman Coulter system, achieving a 100% pass rate for both VCN analysis (DNA) and transgene expression analysis (RNA) across 10 tissue types. Magnetic bead DNA/RNA co-extraction from tissue is standard in gene therapy pharmacology and toxicology studies, with the Zymo Quick-DNA/RNA MagBead Kit among the most cited formats in published methods papers and regulatory submission CMC sections for AAV gene therapies.
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<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP Inc.</a> today and get reliable mag bead systems for automated lab workflows.

Mag Beads

MSDS

DataSheet

Protocol

Quick-DNA/RNA™ MagBead

Quick-DNA/RNA MagBead by Zymo Research — magnetic bead co-purification of genomic DNA (≥40 kb) and total RNA (including microRNA ≥17 nt) from cells, tissue, FFPE, blood, and environmental samples. Binding capacity 15 µg/30 µl beads; ≥50 µl elution; A260/A280 &gt;1.8. Available as R2130 (96 preps) and R2131 (4×96 preps).

Binding Capacity

Equipment Needed

Purity

Recommended Materials

Sample Preservation

Sample Sources

Size Limits

Storage

Manufacturer

Shipping Condition

Storage Condition

Quick-DNA/RNA™ MagBead (96 Preps.)

Quick-DNA/RNA™ MagBead (4 x 96 Preps.)

<h2>What are Spin-Column DNA/RNA Co-Extraction Kits for Cells, Tissue, and Blood?</h2>
&nbsp;
Spin-column DNA/RNA co-extraction kits for cells, tissue, and blood simultaneously purify genomic DNA and total RNA in separate fractions from the same mammalian sample using a sequential two-column silica-membrane workflow in a standard microcentrifuge. The Zymo Quick-DNA/RNA Miniprep Plus Kit loads the sample lysate onto a Zymo-Spin IIICG column that physically captures gDNA while RNA passes through to a Zymo-Spin IIC column for binding, washing, and elution - completing both DNA and RNA purification in under 15 minutes for cell pellets and 45-75 minutes (including Proteinase K digestion) for solid tissue biopsies. Choose the spin-column format for DNA/RNA co-extraction from cells, tissue, and blood when processing 1-24 samples per run, when automation infrastructure is not available, or when the fastest possible DNA + RNA turnaround from a limited biopsy sample is required.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Quick-DNA/RNA&trade; Miniprep Kit for rapid co-extraction of total DNA and RNA from blood and tissues utilizing advanced spin-column technology
</li>
<li dir="ltr" aria-level="1">
Quick-DNA/RNA&trade; Microprep Plus Kit to recover high-purity nucleic acids from extremely small or rare samples using a specialized micro-elution column
</li>
<li dir="ltr" aria-level="1">
Quick-DNA/RNA Miniprep Plus Kit to ensure high-yield genomic DNA and total RNA yields using an ultra-clean spin-column workflow
</li>
<li dir="ltr" aria-level="1">
Blood RNA Mini Kit for the specific extraction of intact viral and total host RNA from whole blood samples using robust silica-membrane columns
</li>
</ul>
&nbsp;
<h2>How to Choose a Spin-Column DNA/RNA Co-Extraction Kit for Cells, Tissue, and Blood</h2>
&nbsp;
Miniprep Plus vs. Microprep Plus Format
The Miniprep Plus kit handles standard inputs (up to 5x10^6 cells or 30 mg tissue) and elutes in 25-100 ul per fraction, appropriate for most downstream applications. The Microprep Plus kit is designed for very low inputs -- 500 cells to 5,000 cells or 0.5-5 mg tissue -- with minimum elution volumes of 6-10 ul per fraction for maximum concentration from limited material. Use Microprep Plus for needle biopsy cores, laser-capture microdissected cells, single-well organoid lysates, and sorted immune cell populations.
DNA/RNA Shield for Sample Lysis and Stabilization
DNA/RNA Shield in the Miniprep Plus and Microprep Plus kits performs dual duty: it lyses cells on contact while simultaneously stabilizing DNA and RNA at room temperature, enabling sample collection and processing to be decoupled by hours or days without cold-chain handling. For tissue biopsies collected in the operating room that cannot be processed immediately, submerging in DNA/RNA Shield preserves RNA integrity for up to 30 days at room temperature -- the practical alternative to snap-freezing in liquid nitrogen.
Tissue Digestion Strategy
Soft, high-cellularity tissues (liver, spleen, kidney cortex) lyse completely in DNA/RNA Shield + Proteinase K at 55 degrees C within 30 minutes. Fibrous tissues (skeletal muscle, heart, skin, lung) require 1-2 hours of Proteinase K digestion or mechanical homogenization (bead-beating, tissue grinder) before column loading. For very fibrous tissue that resists Proteinase K digestion, add bead-beating in a lysis tube (0.5 mm beads) with DNA/RNA Lysis Buffer before proceeding to column loading.
On-Column DNase I for the RNA Fraction
Always include on-column DNase I treatment for RNA-seq and for RT-qPCR assays without intron-spanning primers. Skip DNase I only for RT-qPCR with exon-spanning primer pairs that distinguish cDNA from gDNA amplicons. The IIICG column physically depletes the majority of gDNA but does not eliminate 100%; residual gDNA in the RNA fraction produces false-positive amplification in RT-qPCR assays without an intron-spanning design.
Elution Strategy for Downstream Applications
For RNA-seq: elute RNA in 50-100 ul, quantify by Qubit RNA HS Assay, and verify RIN &gt;= 7 on a Bioanalyzer before library preparation. For RT-qPCR: elute in 25-50 ul for higher RNA concentration. For gDNA applications requiring &gt;= 5 kb fragments (WGS, WES, long-read sequencing): elute DNA in 50-100 ul and quantify by Qubit dsDNA BR Assay; avoid vortexing gDNA eluates to preserve high-molecular-weight fragment integrity.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The Zymo Quick-DNA/RNA Miniprep Plus Kit produces gDNA (A260/A280 &gt;= 1.8, high molecular weight &gt;= 20 kb) and total RNA, including small RNAs &gt;=17 nt (A260/A280 &gt;= 1.8, RIN &gt;= 7 from fresh tissue) in under 15 minutes from cell pellets, with both analytes ready for next-generation sequencing without further cleanup. The Quick-DNA/RNA Miniprep Plus Kit is among the most widely used spin-column co-extraction kits in academic molecular biology, gene therapy, pharmacology, and cancer multi-omics research; its use is cited in published studies at institutions including Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center for paired genomic and transcriptomic analyses. For needle biopsy or LCM inputs where the Miniprep Plus minimum input cannot be met, the Microprep Plus format recovers both analytes from the single-cell level without carrier RNA or pre-amplification requirements.
&nbsp;
For guidance on selecting the right spin-column co-extraction format for your input type or for pricing, <a href="https://mbpinc.net/contact-us">contact the expert team at MBP Inc. </a>

Spin-column

Quick-DNA/RNA™ Microprep Plus Kit

D7005 Quick-DNA/RNA Microprep Plus Kit by Zymo Research co-purifies DNA (≥40 kb) and RNA (including microRNA) from cells, tissue, blood, FFPE, and environmental samples in a single spin-column workflow. 50 preps, room-temperature storage.

Equipment

Sample Source

Size Range

Yield

Quick-DNA/RNA™ Microprep Plus Kit (50 Preps.)

Quick-DNA/RNA™ Microprep Plus Kit (10 Preps.)

Quick-DNA/RNA™ Miniprep

Quick-DNA/RNA™ Miniprep Kit (D7001) by Zymo Research — 50 preps, simultaneous DNA and RNA purification in &lt;15 min. Up to 100 µg DNA / 50 µg RNA per prep; A260/A280 &gt;1.8; DNA ≥40 kb, RNA ≥17 nt.

Quick-DNA/RNA™ Miniprep (50 Preps.)

DNA/RNA Lysis Buffer (200 ml)

DNA/RNA Lysis Buffer (50 ml)

DNA Prep Buffer (10 ml)

DNA Prep Buffer (25 ml)

<h2>What are Spin-Column Environmental/Microbial DNA/RNA Co-Extraction Kits?</h2>
&nbsp;
Spin-column environmental/microbial DNA/RNA co-extraction kits simultaneously purify genomic DNA and total RNA - including small RNAs 17-200 nt - from feces, soil, water, biofilm, swabs, plant material, and body fluids using bead-beating mechanical disruption with BashingBeads (0.1 mm + 0.5 mm) for unbiased lysis of all microbial cell types, followed by sequential Zymo-Spin column purification in a standard microcentrifuge. The ZymoBIOMICS DNA/RNA Miniprep Kit delivers DNA and RNA in separate fractions - the primary advantage over the combined total nucleic acid eluate of the MagBead format - enabling parallel metagenomic and metatranscriptomic library preparation from one undivided environmental input without post-extraction enzymatic separation steps. Choose the spin-column format when processing 1-24 environmental samples per run, when separate DNA and RNA fractions are required from the outset, or when automation infrastructure is unavailable.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Quick-DNA/RNA&trade; Water Kit to isolate high-purity nucleic acids from water samples using specialized column purification
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Quick-DNA/RNA&trade; Pathogen MiniPrep Kit for rapid co-extraction of pathogen genomes utilizing robust spin-column technology
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
ZymoBIOMICS&trade; DNA/RNA Mini Kit to ensure unbiased microbiome profile recoveries using an innovative lysis system&nbsp;
</li>
</ul>
&nbsp;
<h2>How to Choose a Spin-Column Environmental/Microbial DNA/RNA Co-Extraction Kit</h2>
&nbsp;
Separate vs. Combined Fractions
The ZymoBIOMICS DNA/RNA Miniprep natively delivers separate DNA and RNA by column switching: gDNA binds the IIIC column while RNA flows through to the IC column. No post-extraction enzymatic treatment is needed to separate the analytes. This is the critical differentiator from the MagBead format, which requires DNase I treatment of the combined eluate to prepare an RNA fraction for RNA-seq.
PCR Inhibitor Removal Validation
Always measure A260/A230 on both eluates after extraction. Values below 1.5 indicate inhibitor carryover from humic acids (soil) or bile salts (feces) that will suppress PCR amplification and NGS library preparation. If A260/A230 &lt; 1.5, reduce input mass to 50-100 mg and add an optional pass through Zymo-Spin III-HRC Inhibitor Removal Columns on the RNA eluate before downstream use.
Bead-Beating Instrument
A vortex with a horizontal adapter (Vortex-Genie 2 at max speed x 5 min) validates for standard fecal and soil samples. A dedicated bead mill (Precellys, FastPrep-24) at 6,000-6,500 rpm for 40-60 seconds per cycle provides more consistent lysis for tough-walled Gram-positive organisms in high-diversity environmental samples; use 2-3 cycles with 30-second cooling intervals on ice to prevent RNA degradation from heat.
DNA/RNA Shield for Sample Stabilization
DNA/RNA Shield preserves microbial community RNA at room temperature for 30 days. Add Shield directly to the sample tube immediately after collection - before any transport or refrigeration delay - to capture the metabolic RNA expression state at the moment of collection. Shield-stabilized samples load directly into BashingBead Lysis Tubes without additional preparation.
Input Scaling for Low-Biomass vs. High-Biomass Samples
Standard input for feces: 250 mg. Standard input for topsoil: 200 mg. For low-biomass environmental samples (filtered water, air, oligotrophic deep soil): collect maximum biomass by filtering the largest feasible volume and add carrier RNA (5 ng per extraction) to the lysis step to improve column binding of trace nucleic acids. For high-biomass samples (feces, compost), reduce input to 50-100 mg to avoid column overloading and inhibitor carryover.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Performance benchmarks for the ZymoBIOMICS DNA/RNA Miniprep Kit: DNA yield 2-15 ug from 250 mg feces (A260/A280 &gt;= 1.8, fragment size &gt;= 15 kb for standard input); RNA yield 2-10 ug from 250 mg feces (A260/A280 &gt;= 1.8, A260/A230 &gt;= 1.5, small RNAs 17-200 nt included). The kit uses Zymo-Spin column technology with OneStep PCR Inhibitor Removal Technology validated for feces, soil, plant material, water, biofilm, swabs, saliva, and body fluids, producing inhibitor-free nucleic acids ready for RT-PCR, arrays, sequencing, and microbiome analyses. The ZymoBIOMICS DNA/RNA Miniprep is widely used in gut microbiome and soil metatranscriptomics research at institutions including Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center.
&nbsp;
For bulk pricing on ZymoBIOMICS DNA/RNA Miniprep kits, bead-beating instrument recommendations, or protocol guidance for low-biomass or high-inhibitor environmental samples,&nbsp;<a href="https://mbpinc.net/contact-us">contact the MBP team</a>.

Quick-DNA/RNA™ Pathogen MiniPrep Kit

The Quick-DNA/RNA&trade; Pathogen MiniPrep Kit (R1043) by Zymo Research is a 200-preparation spin-column kit for simultaneous isolation of DNA and RNA from vector-borne pathogens including viruses, bacteria, and protozoa. Compatible vectors include mosquitoes (&le;50 per prep), ticks (1 engorged or &le;5 flat adults or &le;20 nymphs), fleas (&le;10), and deer flies (1 adult), as well as mammalian and avian tissue (&le;10 mg).
Binding capacity: 50 &micro;g per column; size range: 50 nt to ~200 kb; elution: &ge;25 &micro;L (standard 50 &micro;L). All components store at room temperature. The 50-prep version is R1042.
MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, Eurofins, and many other institutes across the USA. Order via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or contact <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.

Quick-DNA/RNA™ Pathogen MiniPrep (200 Preps.)

Quick-DNA/RNA™ Pathogen MiniPrep (50 Preps.)

<h2>What are Spin-Column Viral DNA/RNA Co-Extraction Kits?</h2>
&nbsp;
Spin-column viral DNA/RNA co-extraction kits purify viral DNA and RNA simultaneously from plasma, serum, urine, nasopharyngeal swabs, saliva, CSF, and cell culture media using a single-buffer lysis that disrupts viral particles and conditions nucleic acids to bind a Zymo-Spin silica-membrane column in a standard microcentrifuge. Both DNA and RNA elute together in 30-100 ul of DNase/RNase-free water, ready for multiplex RT-qPCR targeting RNA viruses (SARS-CoV-2, influenza A/B, RSV, HIV) and DNA viruses (adenovirus, herpesvirus, HPV, parvovirus) from the same eluate in a single assay. Choose the spin-column viral DNA/RNA format when processing fewer than 50 samples per run, when only a microcentrifuge is available, or when the fastest possible turnaround from sample to detectable eluate is required for point-of-care adjacent testing.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Quick-DNA/RNA&trade; Viral Kit (D7020): Efficient spin-column technology designed for the fast co-purification of high-quality viral RNA and viral DNA from liquid specimens.
</li>
<li dir="ltr" aria-level="1">
Virus RNA Kit (R6620-00): Specialized silica-membrane columns created for sensitive extraction of viral RNA, guaranteeing pure yields for subsequent detection processes.
</li>
<li dir="ltr" aria-level="1">
MV Total RNA Isolation (R6313-01): Custom extraction columns designed for the retrieval of total RNA from difficult molecular biology samples, ensuring reliable recovery.
</li>
</ul>
&nbsp;
<h2>How to Choose a Spin-Column Viral DNA/RNA Co-Extraction Kit</h2>
&nbsp;
Single-Buffer vs. Multi-Buffer Lysis
Zymo Quick-DNA/RNA Viral Kit uses a single-step lysis buffer -- no protease incubation, no carrier RNA addition, no ethanol mixing before column loading. This reduces protocol complexity and per-batch error rate. Multi-buffer kits (QIAGEN QIAamp, Thermo PureLink) require preparation of lysis buffer + carrier RNA + ethanol before loading; they are comparable in yield but add setup time per batch.
Input Volume Flexibility
Standard spin-column viral kits accept 50-200 ul of cell-free sample. For low-titer samples requiring larger input volumes to increase sensitivity, check the kit for extended-input protocols; some kits allow 400-1,000 ul plasma with vacuum manifold loading. For samples in the DNA/RNA Shield, direct loading is validated without dilution.
DNA+RNA vs. RNA-Only Recovery
Confirm you need co-extraction before ordering. If your panel includes only RNA viral targets (influenza, RSV, SARS-CoV-2), an RNA-only viral spin-column kit produces equivalent sensitivity with a simpler protocol. Co-extraction is essential when your panel includes HPV, adenovirus, CMV, EBV, parvovirus, or any other DNA virus target - RNA-only kits will under-recover or miss these targets.
Elution Volume and Downstream Assay
Elute in 30-50 ul for RT-qPCR sensitivity; the higher nucleic acid concentration produces Ct improvement of 0.5-1.5 cycles compared to 100 ul elution. For multiplex NGS library preparation, elute in 100 ul and quantify by Qubit dsDNA BR and RNA HS assays separately before library input normalization.
Throughput Scaling
When daily volumes exceed 24 samples, the spin-column format creates centrifuge bottlenecks. At &gt;50 samples per run, transition to the Quick-DNA/RNA Viral 96-well plate format (vacuum manifold) or Quick-DNA/RNA Viral MagBead format (automation) for higher throughput.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Performance benchmarks for spin-column viral DNA/RNA co-extraction: &gt;=70% recovery of RNA and DNA spike-in controls, Ct within 2 cycles of reference extraction for validated viral targets, and A260/A280 &gt;= 1.7 in eluates. The Zymo Quick-DNA/RNA Viral Kit uses a single buffer system validated for enteroviruses, rhinoviruses, coronaviruses (SARS-CoV-2), HIV, HCV, influenza A, flaviviruses (dengue, Zika), measles, parainfluenza, and parvovirus; the kit is used in Zymo's SARS-CoV-2 extraction workflow and has been adopted in published multiplex respiratory panel methods. Spin-column viral co-extraction kits are the standard format in clinical research labs processing 10-50 patient samples per run for research-use multiplex RT-qPCR panels at institutions including Vanderbilt University and MD Anderson Cancer Center.
&nbsp;
For kit recommendations, bulk pricing on Quick-DNA/RNA Viral spin-column kits, or guidance on pairing with multiplex RT-qPCR panels,&nbsp;<a href="https://mbpinc.net/contact-us">contact the MBP team</a>. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center.

Spin-Column

MSDS - D7020-1

Quick-DNA/RNA™ Viral Kit

D7020 by Zymo Research co-isolates viral DNA and RNA from up to 400 µL of plasma, serum, urine, blood, saliva, or swab samples in ~5–10 minutes hands-on time. The included DNA/RNA Shield™ 2X lyses viral particles, inactivates infectious agents, and stabilizes nucleic acids at ambient temperature—supporting safe room-temperature sample transport. Eluate is ready for NGS, RT-qPCR, and hybridization detection.

Quick-DNA/RNA™ Viral Kit (50 Preps.)

Viral DNA/RNA Buffer (100 ml)

Viral DNA/RNA Buffer (25 ml)

Quick-DNA/RNA™ Viral Kit (200 Preps.)

Quick-DNA/RNA™ Water Kit (50 Preps)

R2044 by Zymo Research is a 50-prep kit for simultaneous DNA and RNA recovery from water, wastewater, and sludge samples. It processes up to 1 L (low-biomass water) or 50 mL (raw wastewater), yielding up to 25 µg DNA and 100 µg RNA per prep at A260/A280 >1.8 without vacuum filtration. Industry scientists supporting wastewater surveillance, environmental virology, or drinking water monitoring benefit from the inhibitor-removal technology and ddPCR/RT-qPCR/NGS-ready eluates.

Sample Size

DNA/RNA Purity

DNA/RNA Storage

Equipment Needed (user provided)

<h2>What are FFPE DNA extraction kits?</h2>
&nbsp;
An FFPE DNA extraction kit purifies genomic DNA from formalin-fixed, paraffin-embedded tissue sections through a deparaffinization step (xylene-free in most current kits), proteinase K digestion to lyse fixed tissue, heat treatment to reverse formalin-induced crosslinks, and silica-membrane spin column purification, with some kits offering an optional uracil-N-glycosylase (UNG) treatment to remove deaminated-cytosine artifacts before NGS. Choose an FFPE-specific kit rather than a standard tissue kit whenever your sample is formalin-fixed and paraffin-embedded, since standard kits don't include deparaffinization or crosslink-reversal steps.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">Quick-DNA&trade; FFPE MiniPrep for rapid isolation of high-purity genomic DNA from FFPE tissue samples for high-throughput downstream applications.</li>
<li data-section-id="1n7706u" data-start="258" data-end="375">Designed to recover highly fragmented and cross-linked DNA suitable for sensitive downstream molecular workflows.</li>
<li data-section-id="355glt" data-start="376" data-end="494">Streamlined workflow supports rapid processing of FFPE samples with minimal hands-on time and reduced sample loss.</li>
<li data-section-id="125uabh" data-start="495" data-end="611">Optimized for compatibility with high-throughput applications, including PCR, sequencing, and genotyping assays.</li>
<li data-section-id="10hv3na" data-start="612" data-end="731" data-is-last-node="">Ensures consistent DNA yield and purity from challenging archived tissue specimens for reliable experimental results.</li>
</ul>
&nbsp;
<h2>How to choose an FFPE DNA extraction kit</h2>
&nbsp;
Deparaffinization chemistry
Most current FFPE DNA kits use a proprietary, xylene-free Deparaffinization Solution that dissolves paraffin without multiple wash steps, though some published methods use mineral oil as a simple alternative deparaffinization approach. Xylene-free chemistry reduces hands-on time and avoids handling a hazardous solvent, which matters for labs without dedicated solvent fume hoods.
Crosslink reversal and artifact removal
Formalin fixation creates DNA-protein crosslinks that block PCR unless reversed, so FFPE kits include a heat-based de-crosslinking step after lysis; some kits add an optional UNG treatment before a second lysis step to remove deaminated-cytosine artifacts, which is specifically useful for reducing C-to-T and G-to-A transition artifacts in NGS data. If your downstream application is NGS or dPCR on long-archived samples, a kit with UNG treatment is worth the extra step.
Sample input and elution volume
FFPE kits typically recommend 6-8 tissue sections of 5-10 um thickness as input, with some kits offering elution volumes as low as 15-30 ul for concentrated DNA suitable for limited-input downstream assays. If your archived sections are scarce, prioritize kits that minimize sample loss during deparaffinization rather than requiring multiple wash steps.
Expected DNA quality and fragment size
DNA from FFPE samples is inherently fragmented due to formalin fixation, and downstream applications involving fragments under 200 bp are generally achievable with effective deparaffinization and extraction; PCR success rates around 97% across microsatellite markers have been reported with optimized protocols on long-archived specimens. Set expectations for fragment size and PCR success rate based on sample age and fixation duration rather than assuming fresh-tissue-equivalent quality.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for FFPE DNA extraction are deparaffinization chemistry (xylene-free vs. solvent-based), whether crosslink reversal and optional UNG artifact removal are included, and elution volume relative to your sample's scarcity. A kit recommending 6-8 sections per extraction with elution as low as 15-30 ul concentrates limited archived material for downstream PCR or NGS. Labs working with both fresh and archived samples often keep&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/blood/tissue">blood/tissue genomic DNA kits</a>&nbsp;for fresh/frozen tissue alongside a dedicated FFPE kit for archived specimens, since standard tissue kits lack deparaffinization chemistry. The parent&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits hub</a>&nbsp;and broader&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits">DNA extraction kits hub</a>&nbsp;link to related sample-type categories, including&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/urine">urine DNA extraction kits</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/insect">insect genomic DNA kits</a>&nbsp;for other specialized sample types.&nbsp;
&nbsp;
<section class="mbp-contact-cta">
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

FFPE

Quick-DNA™ FFPE MiniPrep

D3067 by Zymo Research is the Quick-DNA FFPE MiniPrep Kit (50 preps) for extracting up to 25 µg of DNA from FFPE tissue samples into ≥50 µl with A260/A280 ≥1.8 purity. Includes Deparaffinization Solution and Zymo-Spin IIC columns; fragment size selection available for &gt;50 bp (qPCR) or &gt;500 bp (NGS) targets. Compatible with fresh/frozen tissue. Requires microcentrifuge and heat block (55 °C and 90 °C). Made in USA by Zymo Research.

Applicable For

Processing Volume

Sample Storage

Type

Quick-DNA™ FFPE MiniPrep (50 Preps.)

Deparaffinization Solution (20 ml)

Genomic DNA Wash 1 (25 ml)

Genomic DNA Wash 2 (12 ml)

<h2>What are microbial and environmental DNA isolation kits?</h2>
&nbsp;
Microbial and environmental DNA isolation kits purify DNA from soil, sediment, freshwater, marine water, feces, and other complex environmental matrices using lysis and purification chemistry designed to remove humic acids, metal compounds, and other inhibitors that interfere with PCR and sequencing, supporting 16S/18S rRNA amplicon profiling and shotgun metagenomic sequencing. Related entities include "kitome" and "splashome" contamination controls, alpha diversity and microbial community composition analysis, and compatibility with both short-read (Illumina) and long-read (Oxford Nanopore) sequencing platforms. Choose a kit validated for your specific sample matrix (soil, sediment, water, or feces), since benchmarking studies consistently show kit performance varies by matrix rather than one kit being universally best.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Spin-column fungal and bacterial kits for rapid DNA purification using rapid silica-membrane technology&nbsp;</li>
<li dir="ltr" role="presentation">Parallel extraction of soil, fecal, and environmental samples using high-throughput 96-well microplates&nbsp;</li>
<li dir="ltr" role="presentation">Automated&nbsp; purification of complex microbial and environmental specimens using Magnetic bead isolation systems</li>
<li dir="ltr" role="presentation">Scalable microprep and midiprep columns tailored for concentrated low-volume elution demands and diverse sample inputs</li>
</ul>
&nbsp;
<h2>How to choose a microbial and environmental DNA isolation kit</h2>
&nbsp;
Matrix-specific kit performance
Benchmarking studies comparing multiple commercial kits across sediment, water, soil, oyster gut microbiome, and mammalian feces have found that the best-performing kit varies by matrix - kits ranked highly for sediment did not necessarily perform best for water or host-associated samples. Rather than assuming a single "universal" kit, match your kit choice to published benchmarking results for your primary sample type where available.
Inhibitor removal for downstream PCR and sequencing
Environmental samples, especially sediments and soils, contain organic and metal compounds that inhibit PCR amplification if not removed during extraction; kits designed for these matrices integrate inhibitor-removal steps as part of the standard protocol. If your samples have historically produced low PCR amplification efficiency with general-purpose kits, an environmental-matrix-specific kit with built-in inhibitor removal is the first thing to evaluate.
Short-read vs. long-read sequencing compatibility
For long-read metagenomic sequencing, DNA fragment length and integrity from the extraction kit directly affect achievable read lengths, though longer extracted fragments don't always translate consistently into longer sequencing reads - kit choice affects both fragment length and downstream classification rate and reproducibility. If long-read metagenomics is your goal, prioritize kits with published long-read benchmarking data over kits validated only for short-read amplicon work.
Contamination control (kitome/splashome)
Kit-derived contamination ("kitome") and cross-sample contamination during processing ("splashome") can affect microbial community composition results, particularly for low-biomass samples; benchmarking studies evaluate kits specifically for these contamination sources alongside yield and diversity metrics. For low-biomass environmental samples, prioritize kits with documented low kitome contamination in published comparisons.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for microbial and environmental DNA isolation are matrix-specific validation (soil vs. sediment vs. water vs. feces), built-in inhibitor removal for PCR-ready output, and fragment length/integrity for long-read sequencing compatibility where relevant. Since benchmarking studies repeatedly show no single kit performs best across all matrices, identifying your primary sample type before selecting a kit is the most impactful decision. Labs running metagenomic surveys across multiple matrices (e.g., soil and water from the same field site) often need separate matrix-optimized kits rather than a single universal kit. The parent <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits hub</a>&nbsp;links to related sample-type categories, including&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/insect">insect genomic DNA kits</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/plant">plant genomic DNA kits</a>&nbsp;for organism-associated environmental work, and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/high-molecular-weight-dna">high molecular weight DNA kits</a>&nbsp;for long-read metagenomics requiring larger fragment sizes. For cell-free DNA in environmental water samples (eDNA detection), see&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/cell-free-dna">cell-free DNA kits</a>.&nbsp;
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Microbial & Environmental DNA Isolation

Quick-DNA™ Fecal/Soil Microbe 96 Kit (2 x 96 Preps.)

The Quick-DNA™ Fecal/Soil Microbe 96 Kit (D6011) by Zymo Research extracts inhibitor-free genomic DNA in a 96-well format from fecal samples (≤80 mg), soil (≤135 mg), and microbial cells. Silicon-A-HRC technology removes humic acids, polyphenolics, and melanins. Yields up to 5 µg DNA per prep at A260/A280 ≥1.8; 2 × 96 preps per kit; compatible with qPCR, NGS, and microarrays.

Elution Volume

MSDS - D6010-FM

MSDS - D6011-FM

MSDS - D6012-FM

Optimized Lysis Protocols

Quick-DNA™ Fecal/Soil Microbe 96 Magbead Kit

Quick-DNA™ Fecal/Soil Microbe 96 Magbead Kit (D6010FM) by Zymo Research — 96-well magnetic bead DNA extraction from ≤80 mg feces or ≤135 mg soil. Inhibitor-free, ≥37.5 µl elution, ≤25 µg yield, ~90 min processing. Automation-compatible (Hamilton, KingFisher, Tecan). 2 × 96 preps per kit. Made in the USA.

Quick-DNA™ Fecal/Soil Microbe 96 Magbead Kit (2 x 96 Preps) (includes ZR BashingBead™ Lysis Rack)

Quick-DNA™ Fecal/Soil Microbe 96 Magbead Kit (2 x 96 Preps.)(Lysis Matrix Not Included)

Quick-DNA™ Fecal/Soil Microbe 96 Magbead Kit (2 x 96 Preps) (includes ZR BashingBead™ Lysis Tubes)

Quick-DNA™ Fecal/Soil Microbe DNA MiniPrep Kit (50 Preps.)

D6010 by Zymo Research is the Quick-DNA™ Fecal/Soil Microbe DNA MiniPrep Kit (50 preps). Isolates inhibitor-free DNA from feces (≤150 mg), soil (≤250 mg), or fungal/bacterial cells (≤100 mg). Yield ≤25 µg; purity A260/A280 ≥1.8; fragments up to 40 kb+. Uses ZR BashingBead™ Lysis Tubes. Processing time ~15 min after bead beating.

Quick-DNA™ Fecal/Soil Microbe MidiPrep (25 Preps.)

D6110 Quick-DNA Fecal/Soil Microbe MidiPrep Kit by Zymo Research — 25 preps, ≤375 mg feces or ≤5 g soil input, up to 125 µg DNA yield, ≥150 µl elution, ≤25 min, inhibitor-free.

Quick-DNA™ Fungal/Bacterial 96 Kit (2 x 96 Preps.)

D6006 Quick-DNA Fungal/Bacterial 96 Kit by Zymo Research — 2×96 preps, BashingBead lysis, ≤40 min, ≤5 µg yield per well, 25–35 kb fragments, A260/A280 ≥1.8, qPCR and NGS ready.

Processing Time

United States

Texas

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