Zymo Reagents

<h2>What are insect spin-column genomic DNA kits?</h2>
&nbsp;
An insect spin-column genomic DNA kit pairs proteinase K-based lysis with a single silica-membrane spin column to purify genomic, mitochondrial, or microbial DNA from one whole insect or dissected insect tissue sample at a time, processed on a standard microcentrifuge, with optional mechanical disruption (bead beating, grinding) for chitin-rich specimens before the lysis step. Related entities include COI barcode gene amplification, high molecular weight DNA output for long-read sequencing, and multi-organism kits validated for insects alongside blood, tissue, bacteria, and yeast. Choose the spin-column format for individual insect specimens - whether for one-off identification or method development before scaling to batch processing.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>Rapid benchtop processing using silica-membrane spin-column insect DNA kits</li>
<li>Flexible invertebrate matrix handling using advanced insect extraction spin-columns</li>
<li>High-purity downstream templates using inhibitor-free insect genomic DNA purification units</li>
</ul>
&nbsp;
<h2>How to choose an insect spin-column genomic DNA kit</h2>
&nbsp;
Matching the lysis approach to the specimen type
Soft-bodied insects, larvae, or dissected internal tissue generally lyse adequately with proteinase K digestion alone, similar to standard animal tissue protocols, while hard-bodied or larger whole insects with significant chitin content often need mechanical disruption (bead beating, grinding, or homogenization) before or alongside lysis. Identify your specimen's exoskeleton characteristics before assuming the base proteinase K protocol applies without pretreatment.
Whole specimen vs. tissue dissection
Processing a whole small insect (e.g., Drosophila) in a spin-column kit is convenient and commonly documented, while larger insects may benefit from dissecting leg or thoracic muscle tissue to reduce the chitin-to-soft-tissue ratio in the lysate, improving DNA purity per unit input. Choose based on specimen size and whether dissection time is acceptable for your throughput needs.
Single-organism vs. multi-organism kit chemistry
Some spin-column kits are validated across multiple organism types - blood, tissue, bacteria, yeast, and insects - within one kit chemistry, useful for labs handling diverse sample types without switching kits. Confirm the kit's documentation explicitly addresses insects (and ideally your specific taxon) rather than relying on a general "tissue" claim.
DNA quality for downstream barcoding vs. sequencing
For COI barcoding and standard PCR, moderate-integrity DNA from a standard proteinase K protocol is generally sufficient, while population genetics or genome assembly projects benefit from kits documented to produce high molecular weight DNA (peak sizes around 50 kb or higher). Match your kit choice to the downstream application's DNA integrity requirements rather than defaulting to the highest-spec kit for routine barcoding.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The specs that most affect an insect spin-column kit choice are whether mechanical disruption is documented for your specimen's exoskeleton type, whether the kit chemistry is validated for insects specifically or only generically for "tissue," and the expected DNA fragment size relative to your downstream application. A kit suitable for both COI barcoding and population genetics, with peak fragment sizes around 50 kb or higher, covers a broad range of insect molecular work from a single specimen.&nbsp;The parent&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/insect">insect genomic DNA extraction kits category</a>&nbsp;and broader&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits hub</a>&nbsp;link to related formats, and for plant-sample work in pest-related research,&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/plant/spin-column">plant spin-column kits</a> use comparable column chemistry with different lysis buffers.&nbsp;
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Spin-Column

DataSheet

MSDS

Protocol

Quick-DNA™ Tissue/Insect Miniprep Kit (50 Preps.)

D6016 Quick-DNA Tissue/Insect Miniprep Kit by Zymo Research uses BashingBead lysis to extract high-molecular-weight DNA (≤25 µg, 25–35 kb) from ≤50 mg of insects, mammalian tissues, or whole blood. 50 preps.

Applicable For

Elution Volume

Equipment

Purity

Sample Source

Sample Storage

Size Range

Type

Yield

Manufacturer

Shipping Condition

Storage Condition

<h2>What are urine DNA extraction kits?</h2>
&nbsp;
A urine DNA extraction kit purifies cellular and/or cell-free DNA from urine using a stabilization or conditioning buffer to prevent degradation, a precipitation step, chemical lysis and enzymatic digestion, and silica spin-column purification, yielding DNA suitable for qPCR, sequencing, arrays, and DNA methylation analysis. Related entities include cell-free circulating DNA (cfDNA), liquid biopsy biomarker discovery, urine stabilization reagents, and downstream methylation-sensitive PCR. Choose a urine-specific kit over a general blood/tissue kit whenever cell-free DNA recovery or extended ambient storage of urine samples is needed, since cfDNA in untreated urine degrades rapidly.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>Quick-DNA&trade; Urine Kit is designed for high-quality purification of cell-free DNA (cfDNA) and cellular DNA from urine samples</li>
<li>Enables efficient recovery of nucleic acids suitable for sensitive downstream molecular biology workflows</li>
<li>Supports PCR and qPCR-based analysis with high sensitivity and reproducibility</li>
<li>Widely used in biomarker discovery and liquid biopsy research applications</li>
<li>Suitable for non-invasive diagnostic and translational research studies</li>
</ul>
&nbsp;
<h2>How to choose a urine DNA extraction kit</h2>
&nbsp;
Cell-free vs. cellular vs. total DNA
Some urine DNA kits target cell-free DNA only, others cellular DNA only, and some recover both fractions from the same sample with different processing steps; cellular DNA generally requires pelleting cells by centrifugation before lysis, while cell-free DNA requires the supernatant after this step. Identify which fraction your application needs, since cfDNA-focused biomarker work and cellular DNA genotyping have different processing requirements.
Stabilization at collection
Because circulating cell-free DNA in non-stabilized urine is rapidly degraded by high nuclease activity after collection, a stabilization or conditioning buffer added at collection can preserve DNA at ambient temperature for up to about one month; without stabilization, samples should be processed immediately, including a centrifugation step right after collection to separate cellular and cell-free fractions. Plan your collection logistics around whether a stabilization buffer will be available at the point of sample collection.
Input volume and concentration needs
Urine DNA kits vary in input volume capacity, from smaller-volume kits processing 250 ul to 2 mL for cell-free circulating DNA, up to larger-volume kits processing up to 40 mL for combined cellular and cell-free DNA with a precipitation step. Match input volume capacity to your typical collected sample volume rather than diluting or splitting samples across multiple preps.
Downstream application fit
DNA from urine extraction kits is generally compatible with qPCR, methylation-sensitive PCR, microarrays, NGS, and Southern blot analysis, with cfDNA fragment size profiles varying by donor and by extraction kit chemistry. For liquid biopsy applications sensitive to nucleosomal-sized fragments, confirm the kit's reported fragment size distribution matches your assay's requirements.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for urine DNA extraction are which DNA fraction (cellular, cell-free, or both) the kit targets, whether a stabilization buffer is included for ambient storage, and input volume capacity matched to your collection protocol. A kit processing up to 40 ml with a stabilization reagent good for about one month at ambient temperature addresses the core logistical challenge of urine-based cfDNA work - rapid post-collection degradation. Researchers combining urine-based liquid biopsy work with other sample types often keep this category alongside <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/viral">viral DNA extraction kits</a>&nbsp;for plasma/serum-based cfDNA work, since both address cell-free DNA but from different matrices. The parent&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits hub</a>&nbsp;and broader&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits">DNA extraction kits hub</a>&nbsp;link to related sample-type categories, including&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/ffpe">FFPE DNA extraction kits</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/blood/tissue">blood/tissue genomic DNA kits</a>.
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Urine

Quick-DNA™ Urine Kit

D3061 by Zymo Research is the Quick-DNA™ Urine Kit—a 50-prep spin-column system that isolates both cell-free and cellular DNA from up to 40 ml of urine, recovering up to 5 µg in ≥10 µl with A260/A280 ≥1.8. Clearing Beads remove PCR inhibitors and turbidity, and the purified DNA is compatible with qPCR, NGS, bisulfite conversion, and methylation analysis.

Processing Volume

Reagent Storage

Sample Stability

Quick-DNA™ Urine Kit (50 Preps.)

Urine Conditioning Buffer (140 ml)

Urine Conditioning Buffer (8 ml)

Clearing Beads (1 ml)

Urine Pellet Digestion Buffer (20 ml)

Urine DNA Prep Buffer (10 ml)

Urine DNA Wash Buffer (12 ml)

Urine Collection Kit (10 Pack)

Urine Colletion Cup (10 Cups)

<h2>What Are Spin-Column Viral DNA Extraction Kits?</h2>
&nbsp;
Spin-column viral DNA extraction kits combine a chaotropic lysis and binding buffer with a silica-membrane spin column housed in a collection tube, allowing viral DNA to be purified using a standard laboratory microcentrifuge. Following lysis, viral nucleic acids bind to the membrane while contaminants are removed during wash steps before purified DNA is eluted for downstream analysis.&nbsp;Choose a spin-column viral DNA extraction kit when processing low-to-moderate sample volumes and when a standard benchtop microcentrifuge provides a more practical solution than a 96-well plate workflow.
&nbsp;
<h2>What You Will Find</h2>
&nbsp;
<ul>
<li>Obtain high-quality viral nucleic acids using spin-column-based purification for PCR, qPCR, sequencing, and molecular diagnostic applications.</li>
<li>Mini and maxi purification formats for efficient concentration and isolation of adenovirus, lentivirus, retrovirus, and adeno-associated virus (AAV).</li>
<li>Optimized workflows that minimize inhibitors and maximize downstream compatibility for fast, reproducible recovery of viral nucleic acids.</li>
<li>Scalable purification options supporting both low-throughput and high-throughput virology, infectious disease, and molecular biology research.</li>
</ul>
&nbsp;
<h2>How to Choose a Spin-Column Viral DNA Extraction Kit</h2>
&nbsp;
Input Sample Volume and Processing Capacity
Most spin-column viral DNA extraction kits accept up to 400 &micro;L of liquid sample per column, with some protocols allowing the same column to be reloaded to process larger total sample volumes. Confirm that your sample type and volume fall within the manufacturer's recommended specifications.
Centrifuge Compatibility
Spin-column purification workflows generally require centrifugation speeds of approximately 10,000 &times; g or greater. Most standard benchtop microcentrifuges used with 1.5&ndash;2 mL tubes can accommodate these requirements, but equipment compatibility should always be verified before implementation.
Elution Buffer Versus Water
Many kits provide the option of eluting DNA with either a storage buffer or DNase/RNase-free water. Elution buffer may be preferable for longer-term storage, while water can be advantageous for downstream applications sensitive to EDTA or other buffer components.
Sample Type Considerations
Spin-column viral DNA extraction kits are optimized primarily for cell-free liquid samples. Protein-rich or solid biological samples may require additional pretreatment steps or may be better served by dedicated genomic DNA extraction kits designed for blood or tissue specimens.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The most important performance specifications for spin-column viral DNA extraction kits are sample input capacity and minimum elution volume, as these directly influence DNA concentration and recovery efficiency. A workflow processing several hundred microliters of sample and eluting into a smaller final volume can significantly improve target concentration for downstream qPCR analysis.&nbsp;Many laboratories maintain both spin-column and 96-well plate extraction formats to accommodate varying weekly sample volumes. Spin-column systems offer flexibility and simplicity for smaller studies, while maintaining the DNA quality required for PCR, qPCR, sequencing, and molecular diagnostic applications.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the MBP team </a>today to discuss your viral DNA extraction workflow and receive expert guidance on selecting the most suitable spin-column kit for your PCR, qPCR, sequencing, or molecular diagnostics application.

Quick-DNA™ Viral Kit

D3015 Quick-DNA Viral Kit by Zymo Research purifies total DNA (100 bp–50 kb, up to 5 µg) from ≤200 µl of whole blood, plasma, serum, and liquid samples in a spin-column format. 50 preps.

Quick-DNA™ Viral Kit (50 Preps.)

ZR Viral DNA Buffer (50 ml)

Quick-DNA™ Viral Kit (200 Preps.)

ZR Viral DNA Buffer (100 ml)

<h2>What are 16S/ITS NGS library preparation kits?</h2>
&nbsp;
16S/ITS NGS library preparation kits convert extracted microbial DNA into a sequencing-ready library by amplifying a targeted hypervariable region: the 16S ribosomal RNA gene for bacterial and archaeal community profiling, or the ITS (internal transcribed spacer) region for fungal community profiling. Both genes contain conserved flanking sequences that serve as universal PCR primer binding sites, allowing amplification of the variable region in between, which is then sequenced and compared against a reference database for taxonomic classification.
&nbsp;
<h2 dir="ltr">What you will discover:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Quick-16S&trade; Plus Kits (V1&ndash;V4): Enhanced systems for targeted amplification of bacterial 16S rRNA, incorporating validated reagents for accurate classification.
</li>
<li dir="ltr" aria-level="1">
Quick-ITS&trade; Plus Library Prep: Sensitive kits for fungal detection, employing specialized chemistry to capture ITS regions while preserving genomic integrity at its best.
</li>
<li dir="ltr" aria-level="1">
UDI Indexing Integration: Sophisticated indexing methods to avoid barcode hopping and guarantee precise demultiplexing during simultaneous processing.
</li>
<li dir="ltr" aria-level="1">
Accelerated Workflows: Nuclease-free procedures that reduce manual effort while ensuring transfection-grade purity for delicate sequencing systems.
</li>
</ul>
&nbsp;
<h2>How to choose a 16S/ITS library preparation kit</h2>
&nbsp;
Confirm which hypervariable region(s) your kit targets
16S library prep kits commonly target specific hypervariable regions such as V3-V4, and primer sets re-designed against an updated 16S reference database can meaningfully improve taxonomic coverage compared with older primer designs that may underrepresent certain taxa.
Decide between single-region and full-length amplicon coverage
Short-read sequencing of a single hypervariable region, or a small number of regions, is the standard, cost-effective approach, while long-read platforms can sequence the full-length 16S gene, providing improved species- and strain-level resolution at a different cost and throughput tradeoff.
Plan for PCR chimera formation as a known amplicon sequencing challenge
PCR chimeras, artificial sequences formed from recombination of multiple templates during amplification, are a recognized challenge in amplicon library prep, and kits using real-time PCR to control amplification cycle number can help keep chimera abundance to a low, well-controlled percentage of total reads.
Confirm fungal ITS coverage if your study includes fungal community profiling
Some 16S kits can be run alongside a separate ITS-targeted protocol, or as a combined workflow, to capture both bacterial and fungal community composition from the same sample set, which matters for studies where fungi are a relevant part of the community.
Check hands-on time and workflow complexity for your sample throughput
Some 16S library prep kits are designed for a single-tube, single-reaction workflow with simultaneous indexing and amplification, substantially reducing hands-on time and contamination risk compared with multi-step protocols, which matters for higher-throughput sample sets.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
16S rRNA sequencing requires less input DNA and is less impacted by the presence of host DNA than shotgun metagenomic sequencing, which is part of why it remains the more accessible default for routine bacterial community composition profiling across many labs and sample types. As of 2026, single-tube, real-time-PCR-controlled 16S library prep workflows continue to reduce hands-on time for high-throughput studies while keeping PCR chimera formation to a low, well-controlled percentage of total reads.
<a href="https://mbpinc.net/contact-us">Reach out to the MBP Inc. team</a> today to inquire about a quote for your NGS library preparation requirements.

16S/ITS NGS Library Preparation Kits

ITS Guide

Plate Setup Guide

Quick-ITS Plus NGS Library Prep Kit (UDI) (96 preps) with Primer

The Quick-ITS Plus NGS Library Prep Kit UDI D6425-PS1 by Zymo Research delivers 96-prep fungal ITS2 amplicon sequencing with Unique Dual Indexing, ~30 min hands-on time, up to 70% fewer chimeras, and compatibility with Illumina MiSeq and NextSeq platforms.

Sample Input

ITS Primer Sequences (adapters not shown)

Index Primers

Barcode Sequences

Amplicon Size

Sequencing Platform

Required Equipment

Quick-ITS Plus NGS Library Prep Kit (UDI) (96 preps) with Primer Set 1

Quick-ITS Plus NGS Library Prep Kit (UDI) (96 preps) with Primer Set 2

Quick-ITS Plus NGS Library Prep Kit (UDI) (96 preps) with Primer Set 3

Quick-ITS Plus NGS Library Prep Kit (UDI) (96 preps) with Primer Set 4

<h2>What are 96-Well Plate Total RNA Extraction Kits?</h2>
&nbsp;
96-well plate total RNA extraction kits purify total RNA from up to 96 samples in a single processing run using silica-membrane plates, glass-fiber filter plates, or magnetic bead formats compatible with centrifuges, vacuum manifolds, or automated liquid handlers. Unlike single-tube spin-column formats, plate kits maintain individual sample integrity while delivering the throughput needed for screens, longitudinal studies, and genomics pipelines processing hundreds of samples per week.&nbsp;Choose a 96-well plate format when processing 48 or more samples per session to eliminate the per-sample handling overhead of individual spin columns.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Direct-zol&trade;-96 RNA Kits facilitate bypass of the phase-separation steps, enabling phenol-free, high-throughput transcript extraction directly from TRIzol&reg; reagents&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Quick-RNA&trade; 96 Kits for rapid, ultra-pure total RNA delivery from small-scale cellular samples
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
96-well Tissue RNA Kits with multi-well lysis arrays to handle complex, fibrous animal tissues without blocking the filtration matrices
</li>
</ul>
&nbsp;
<h2>How to Choose a 96-Well Plate RNA Extraction Kit</h2>
&nbsp;
Processing Method: Centrifuge vs. Vacuum vs. Magnet
Centrifuge-compatible plates (e.g., QIAGEN RNeasy 96) require a 96-well plate rotor; vacuum manifold protocols skip that equipment dependency. Magnetic bead formats eliminate both and are the preferred choice when an automation platform is available. Select based on the equipment already in your facility before comparing kits.
Sample Type and Starting Amount
Standard plate kits handle 100-2x10^6 cultured mammalian cells or up to 30 mg of tissue per well. Plant tissue extraction requires an additional plant isolation aid to remove polysaccharide and polyphenol inhibitors. Do not use total RNA kits for cell-free samples (plasma, serum, nasal wash) - those require viral RNA formats.
On-Plate DNase Treatment
For RT-qPCR and RNA-seq, choose kits that include on-plate DNase I digestion to remove genomic DNA contamination. Most silica-membrane kits supply lyophilized DNase I; verify that the kit includes this reagent rather than requiring a separate purchase.
Elution Volume and Concentration
96-well kits typically elute in 50-100 ul per well; magnetic bead formats can concentrate into 20-50 ul, which is advantageous for low-abundance samples or applications needing high RNA concentration for library prep.
Automation Compatibility
Magnetic bead 96-well formats integrate with Tecan, Hamilton, and Beckman Coulter liquid handlers using vendor-supplied scripts. Silica-membrane plates can be semi-automated on vacuum manifold platforms. Confirm the kit supplier provides an automation protocol before purchase if robotic processing is required.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Key purity benchmarks for 96-well RNA eluates are A260/A280 &gt;= 1.8 and A260/A230 &gt;= 1.5; values below these thresholds indicate carryover of protein, phenol, or guanidinium salts that inhibit downstream enzymes. Silica-membrane kits from QIAGEN and Bio-Rad remain the most widely cited 96-well formats in peer-reviewed methods, and their protocols are referenced directly in published automation workflows at institutions including the Howard Hughes Medical Institute. RNase-free reagents and consumables are required throughout to prevent RNA degradation; confirm kit buffers are certified RNase-free before use with precious or low-abundance samples.
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

96-well plate

R1052-MSDS

Quick-RNA 96 Kit

The Quick-RNA&trade; 96 Kit (R1052) by Zymo Research purifies total RNA, including small RNAs &ge;17 nucleotides, from cells (up to 106), tissue (up to 5 mg), yeast, plant, and bacteria in a 96-well plate format. R1052 provides 2&times;96-well preparations. Binding capacity: 10 &micro;g RNA per well; elution: &ge;25 &micro;L; purity: A260/A280 and A260/A230 &gt;1.8; protocol time ~30 minutes.
Compatible with DNA/RNA Shield and RNAlater-preserved samples. Optional on-column DNase I treatment is included. Applications: NGS, RT-PCR, microarray, hybridization.
MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, Eurofins, and many other institutes across the USA. Order via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or contact <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.

Quick-RNA™ 96 Kit (2 x 96 Preps.)

Quick-RNA™ 96 Kit (4 x 96 Preps.)

<h2>What are Magnetic Bead Total RNA Extraction Kits?</h2>
&nbsp;
Magnetic bead total RNA extraction kits isolate total RNA by adsorbing nucleic acids onto paramagnetic silica-coated beads in a high-chaotropic-salt lysis buffer, then using a magnetic field to capture and wash beads without centrifugation. Unlike standard spin-column kits, the bead format removes centrifuge dependency, enabling scalable manual processing in 96-well plates or full automation on liquid-handling robots.&nbsp;Choose a magnetic bead format when your lab processes more than 48 samples per day or when a Tecan, Hamilton, or Beckman Biomek platform is available.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Magbio HighPrep Kits for liquid biopsy collection systems and non-invasive swabbing using rapid magnetic bead isolation&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Zymo-Seq RiboFree&trade; Kits to eliminate ribosomal RNA carryover during library synthesis
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Direct-zol-96 MagBead RNA Modules facilitate complete avoidance of manual phase-separation steps while enabling automated transcript extraction directly from TRIzol&reg; lysates&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Magbio HighPrep Total RNA Plus Kit extracts pure, inhibitor-free RNA from complex samples with maximum binding capacity&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Quick-RNA MagBead Packs deliver high-integrity total transcripts without dependence on centrifuge spins or vacuum manifold.
</li>
</ul>
&nbsp;
<h2>How to Choose a Magnetic Bead Total RNA Extraction Kit</h2>
&nbsp;
Automation Compatibility
Confirm that the kit supplier provides automation scripts for your specific platform. The Zymo Research Quick-DNA/RNA Viral MagBead kit and Thermo Fisher MagMAX-96 both offer downloadable instrument scripts for Tecan and Hamilton systems. Generic magnetic bead kits without published automation protocols require in-house script development, adding significant setup time.
Small RNA Retention
Bead kits that bind RNA &gt;=17 nt co-purify microRNAs alongside mRNA and rRNA from the same input. Kits specifying &gt;=200 nt retention exclude small RNAs and are not appropriate for miRNA expression panels. Check the size cutoff stated in the kit datasheet before selecting for non-coding RNA work.
Input Sample Type
Magnetic bead kits for total RNA support cell pellets, tissue homogenates, blood, bacteria, yeast, and fungi. For cell-free matrices (plasma, serum, nasal wash), switch to a viral-RNA or liquid-biopsy magnetic bead kit with included carrier RNA to maximize recovery from low-input, cell-free samples.
Elution Volume and Concentration
Standard magnetic bead kits elute into 50-100 ul; some protocols allow concentration into 20-50 ul, increasing RNA concentration for sensitive downstream applications. If downstream libraries require &gt;=200 ng/ul input, reduce elution volume accordingly and verify the kit supports this protocol variant.
gDNA Removal
Confirm whether the kit includes on-bead DNase I or requires a separate gDNA digestion step. For RT-qPCR and RNA-seq, omitting DNase treatment introduces a genomic DNA signal that inflates expression measurements.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
RNA purity targets from magnetic bead extractions match spin-column benchmarks: A260/A280 &gt;= 1.8 and A260/A230 &gt;= 1.5. RNA integrity number (RIN) &gt;= 7 is recommended for RNA-seq library preparation; magnetic bead kits produce RIN values in the 8-10 range for fresh samples when lysis is performed immediately on ice. Bead-based workflows are cited in published automation protocols from genomics core facilities and are increasingly specified by NIH-funded projects requiring standardized sample processing across multi-site studies.
<section class="mbp-contact-cta"></section>
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP Inc. to get automation-compatible mag bead selections today.

Mag Beads

Quick-RNA MagBead

Quick-RNA™ MagBead R2132 by Zymo Research — 96-prep magnetic bead RNA isolation, RNA ≥17 nt (including miRNA), 15 µg per 30 µL beads, ≥50 µL elution. Inputs: cells, tissue, whole blood, FFPE, environmental, swabs, and DNA/RNA Shield samples. Compatible with liquid handlers and robotic processors; ZR-96 MagStand (P1005) recommended. Order via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.

Binding Capacity

Equipment Needed

Recommended Materials

Sample Preservation

Sample Sources

Size Limits

Storage

Quick-RNA™ MagBead (96 Preps.)

Quick-RNA™ MagBead (4 x 96 Preps.)

<h2>What Are Spin-Column Total RNA Kits?</h2>
&nbsp;
Spin-column total RNA kits are the most widely used format for manual RNA purification in molecular biology laboratories. The technology relies on silica membrane chemistry: under chaotropic (guanidinium-based) lysis conditions, RNA adsorbs selectively to the silica membrane of the column; sequential ethanol-based wash steps remove proteins, salts, and gDNA fragments; and a low-ionic-strength buffer or RNase-free water then releases the purified RNA. Standard mini-format kits accept cell or tissue lysate from inputs of 1x10^4-1x10^7 cells or 5-100 mg tissue, bind up to 100 ug RNA, and complete the protocol in 15-30 minutes. The eluted RNA is suitable for RT-PCR, qRT-PCR, RNA sequencing, Northern blot, and hybridization without additional cleanup.&nbsp;Spin-column RNA kits are the default choice for 1-24 samples per run in any lab that does not require automated magnetic-bead processing.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Quick-RNA Microprep and Miniprep Kits for low-volume elution and highly concentrated recovery, providing ultra-clean and phenol-free transcript isolation
</li>
<li dir="ltr" aria-level="1">
Direct-zol&trade; RNA Kits to bypass tedious phase separation steps, allowing researchers to pump TRIzol-lysed samples directly into spin-columns&nbsp;
</li>
<li dir="ltr" aria-level="1">
Direct-zol&trade; DNA/RNA Parallel Modules are engineered for the non-destructive, simultaneous isolation of DNA and RNA fractions.
</li>
<li dir="ltr" aria-level="1">
ZymoBIOMICS&trade; RNA Mini Kits for uniform microbial profiling featuring host-zeroing lysis mechanics
</li>
<li dir="ltr" aria-level="1">
Quick-RNA&trade; Midiprep Assemblies to capture larger quantities of high-purity total transcripts efficiently, tailored for bulk processing&nbsp;
</li>
</ul>
&nbsp;
<h2>How to Choose Spin-Column Total RNA Kits</h2>
&nbsp;
Column Binding Capacity and Elution Volume
Mini-format columns bind up to 100 ug total RNA and elute in 10-100 uL. For samples with very low RNA content (needle biopsies, laser-capture microdissection sections, small cell numbers &lt;=10^4), choose micro-format columns with 8-12 uL minimum elution volume to maximize concentration. Eluting in &lt;30 uL with high RNA concentration increases sensitivity in downstream RT-qPCR.
Small RNA Retention
This is the most important differentiator between kit brands. Kits using Zymo-Spin column technology with proprietary binding buffers retain miRNA and small RNA species &gt;=17 nt in the total RNA fraction. Competing kits using standard high-ethanol wash chemistry typically lose RNA &lt;200 nt in the wash steps. If your workflow includes small RNA profiling, miRNA RT-qPCR, or small RNA-seq, verify that the kit retains &gt;=17 nt RNA before purchasing.
On-Column DNase Treatment
Included RNase-free DNase I that degrades gDNA on the column during the wash stage eliminates the need for post-elution DNase treatment. This matters for RT-qPCR with primers spanning a small intron, or for RNA-seq where even trace gDNA contamination generates false mapping reads. DNase-treated RNA typically passes a no-RT PCR control at Ct &gt;35.
Sample Type: Cells vs Tissue
Cell lysis buffer volumes and homogenization requirements differ by sample type. Cultured cells lyse directly in lysis buffer with vortexing; tissue samples require homogenization to disrupt cell structure before contact with the lysis buffer. For fibrous tissues (muscle, heart), use a bead-mill homogenizer or rotor-stator; for soft tissues (liver, brain), a disposable pestle or needle shearing is sufficient. Some kits include TissueLyser-compatible bead tubes or QIAshredder-equivalent homogenizer columns.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Spin-column total RNA purity targets: A260/A280 ratio 1.8-2.1; A260/A230 ratio &gt;1.8. RNA integrity number (RIN) from fresh cultured cells or snap-frozen tissue is routinely 8-10 with validated kits. Zymo Research Quick-RNA Mini and Miniprep kits are among the most cited spin-column RNA isolation kits in published RT-PCR and RNA-seq methods; ABM RNA extraction kits offer validated protocols for bacteria, yeast, and fungi not covered by many standard cell/tissue kits. All kits supplied by MBP are Research Use Only (RUO).
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Spin-column

Quick-RNA Microprep Kit

Quick-RNA™ Microprep Kit R1050 by Zymo Research isolates total RNA ≥17 nt — including miRNA — from cells, tissue, yeast, plant, and bacteria using Zymo-Spin IC columns, with ≥6 µL elution and on-column DNase I digestion. Compatible with DNA/RNA Shield and RNAlater; lysed samples stable overnight at RT or at –80°C. Available in 50-prep and 200-prep formats from MBP via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.

Quick-RNA™ MicroPrep Kit (50 Preps.)

Quick-RNA™ MicroPrep (200 Preps.)

Quick-RNA MiniPrep Kit

Quick-RNA MiniPrep Kit by Zymo Research — total RNA isolation in ~10 min with up to 100 µg binding capacity, A260/A280 &gt;1.8, RNA ≥17 nt. DNase I included. Available as R1054 (50 Preps) and R1055 (200 Preps).

Quick-RNA™ MiniPrep Kit (50 Preps.)

Quick-RNA™ MiniPrep Kit (200 Preps.)

MSDS - R1057/R1058

MSDS - R1057T

Quick-RNA Miniprep Plus Kit

R1057 by Zymo Research is the Quick-RNA™ Miniprep Plus Kit (50 preps) for total RNA isolation — including small RNAs ≥17 nt — from cells, all tissue types, whole blood, FFPE samples, and biological fluids. Zymo-Spin IIICG columns with integrated spin-away filters; DNase I and DNA/RNA Shield included. Typical yield 10–15 µg per 10⁶ cells; purity A260/A280 &gt;1.8. Ready for NGS and RT-qPCR.

Quick-RNA™ MiniPrep Plus Kit (50 Preps.)

Quick-RNA™ MiniPrep Plus (10 Preps.)

Quick-RNA™ MiniPrep Plus Kit (200 Preps.)

<h2>What are 96-Well Plate Viral RNA Extraction Kits?</h2>
&nbsp;
96-well plate viral RNA extraction kits purify viral RNA from cell-free biological matrices - plasma, serum, nasopharyngeal swabs, urine, cerebrospinal fluid, saliva, and cell culture supernatant - processing all 96 wells simultaneously using silica-membrane spin plates or magnetic bead 96-well formats compatible with centrifuges, vacuum manifolds, or liquid-handling robots. Unlike single-tube spin-column viral RNA formats that handle 1-24 samples per run, 96-well plate kits deliver the throughput required for batch diagnostic testing, surveillance programs, and large-scale biobank processing.&nbsp;Use a 96-well plate viral RNA kit when processing 48 or more samples per day from cell-free matrices where viral genome copy numbers may be low.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li>Quick-RNA&trade; Viral 96 Kit for rapid, large-scale isolation of viral RNA from plasma, serum, saliva, urine, blood, cell culture media, and cellular suspensions.</li>
<li>High-throughput 96-well spin-plate format for simultaneous processing of large sample batches.</li>
<li>Compatible with samples preserved in DNA/RNA Shield for nucleic acid stabilization during storage and transport at ambient temperatures.</li>
<li>Specialized lysis chemistry designed for efficient recovery of viral RNA from a broad range of RNA and DNA viruses.</li>
<li>Purification workflows that generate high-quality viral RNA suitable for RT-PCR, qPCR, next-generation sequencing, and hybridization-based detection methods.</li>
<li>Streamlined spin-plate extraction technology that supports efficient binding, washing, and elution of viral nucleic acids.</li>
</ul>
<h2>&nbsp;</h2>
<h2>How to Choose a 96-Well Plate Viral RNA Extraction Kit</h2>
&nbsp;
Spin-Plate vs. Magnetic Bead 96-Well Format
Silica-membrane spin-plate kits (e.g., Zymo Quick-RNA Viral 96, Thermo PureLink Pro 96) use centrifugation or vacuum manifold and complete extraction in approximately 30 minutes without a robot. Magnetic bead 96-well formats eliminate centrifugation and integrate with KingFisher Flex, Tecan, and Hamilton platforms; choose beads when a compatible robot is available, and daily volume exceeds 192 samples.
RNA-Only vs. DNA/RNA Co-Extraction
Standard viral RNA spin-plate kits isolate RNA targets. If your assay panel includes both RNA viruses (influenza, SARS-CoV-2, RSV) and DNA viruses (adenovirus, herpesvirus, parvovirus), select a dual-format kit - the Zymo Quick-DNA/RNA Viral 96 Kit co-extracts both from the same 100-200 ul input using a single-buffer lysis system.
Virus Validation List
Confirm the kit's validation data covers your target virus family before adoption. The Zymo Quick-RNA Viral 96 Kit lists validation across coronaviruses, influenza A, flaviviruses, enteroviruses, HIV, HCV, and parvovirus. Kits without published validation for your specific target require in-house validation against a quantified reference standard.
Carrier RNA and Lysis Chemistry
Low-titer specimens benefit from carrier poly(A) RNA in the lysis buffer. Kits using beta-mercaptoethanol as a deproteinization agent improve yield from protein-rich plasma and serum; it is not necessary for simple swab samples. Confirm lysis chemistry matches your sample matrix.
Throughput Beyond 96
For workflows requiring more than 96 extractions per batch, confirm that the kit vendor's plate format is stackable or that the kit supports same-session re-processing without reagent waste.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Performance benchmarks for 96-well plate viral RNA kits: &gt;=70% extraction efficiency by spike-in control, well-to-well Ct variability &lt;=1 cycle, and A260/A280 &gt;= 1.7 in eluates. Standard elution volumes of 50-100 ul per well are suitable for most RT-qPCR assays requiring 2-5 ul input.96-well plate viral RNA extraction is the standard format in accredited clinical microbiology laboratories processing SARS-CoV-2, influenza A/B, and RSV diagnostic panels at volume; workflows built around this format are referenced in published biosafety and QC guidance from institutions, including the Howard Hughes Medical Institute. RNase-free consumables and lysis buffers that inactivate infectious agents at the point of lysis are required for safe batch processing of potentially infectious clinical material.
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

R1040 - MSDS

R1040-Protocol

Quick-RNA Viral 96 Kit

Quick-RNA Viral 96 Kit by Zymo Research is a 96-well spin plate purification system that binds 10 µg RNA per well and elutes in ≥10 µL, recovering 50 nt–200 kb viral RNA from plasma, serum, CSF, swabs, urine, blood, and other sample types — without organic denaturants or proteases. CE-IVD formats available.

Registration Status

Quick-RNA™ Viral 96 Kit (2 x 96 Preps.)

Quick-RNA™ Viral 96 Kit - DX (2 x 96 Preps.)

Quick-RNA™ Viral 96 Kit (4 x 96 Preps.)

Quick-RNA™ Viral 96 Kit - DX (4 x 96 Preps.)

<h2>What are Spin-Column Viral RNA Extraction Kits?</h2>
&nbsp;
Spin-column viral RNA extraction kits isolate RNA from RNA viruses found in cell-free biological fluids by passing the sample through a silica-membrane column in a standard microcentrifuge. A chaotropic lysis buffer simultaneously disrupts viral envelopes, inactivates RNases, and conditions the RNA to bind the silica membrane; successive wash steps remove co-purified proteins, lipids, and PCR inhibitors; and purified viral RNA elutes in 30-60 ul of RNase-free water. The full protocol takes approximately 20 minutes for batches of 1-24 samples, requiring only a microcentrifuge and standard pipetting equipment.&nbsp;Choose a spin-column viral RNA kit when processing fewer than 24 samples per run, when automation infrastructure is unavailable, or when same-day results require the fastest possible turnaround.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li>Quick-RNA&trade; Viral Kit for rapid isolation of high-quality viral RNA from a wide range of transport media and liquid samples.</li>
<li>Advanced silica-membrane spin-column technology for efficient viral RNA binding, washing, and recovery.</li>
<li>Benchtop centrifugal workflows that simplify sample processing with minimal hands-on time.</li>
<li>Viral RNA purification solutions suitable for downstream RT-PCR, qPCR, sequencing, and molecular diagnostic applications.</li>
<li>Reliable extraction of clean viral nucleic acids from clinical, research, and surveillance samples.</li>
<li>Streamlined RNA isolation protocols designed for consistent performance across diverse specimen types.</li>
</ul>
&nbsp;
<h2>How to Choose a Spin-Column Viral RNA Extraction Kit</h2>
&nbsp;
Single-Buffer vs. Multi-Buffer Lysis
Zymo Research Quick-RNA Viral Kits use a single lysis buffer formulation that eliminates reagent preparation steps and reduces handling time. QIAGEN QIAamp Viral RNA Mini Kits use a two-reagent approach (AVL buffer + ethanol) that requires a mixing step before loading. Single-buffer formats reduce pipetting errors in high-frequency diagnostic workflows.
Sample Input Volume
Standard spin-column viral RNA kits accept 140-300 ul per column. For low-titer applications where concentrating a larger plasma volume is needed, verify whether the kit offers extended-volume protocols. QIAGEN's QIAamp Viral RNA Mini Kit specifies 140 ul; Zymo Quick-RNA Viral Kit supports up to 50 ul in direct-column mode with optional elution concentration.
Carrier RNA Inclusion
Kits that include carrier poly(A) RNA in the lysis buffer recover 1-2 additional Ct worth of low-copy viral targets compared to kits without it. Confirm carrier RNA is included; its absence is the most common reason for inconsistent performance on low-viral-load clinical specimens.
DNA Co-Extraction Need
Standard viral RNA spin-column kits do not reliably extract dsDNA viruses. For pan-pathogen applications covering both RNA and DNA viruses from the same sample, select a dual-extraction format (Zymo Quick-DNA/RNA Viral, Thermo PureLink Viral RNA/DNA Mini).
Downstream Application
For RT-qPCR, 30 ul elution volumes concentrate RNA and increase sensitivity. For sequencing library prep requiring larger input mass from low-titer samples, elute in 50-60 ul and pool replicate extractions rather than over-reducing elution volume, which decreases total yield.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Performance benchmarks for spin-column viral RNA kits: extraction efficiency &gt;=70% recovery measured by spike-in controls, eluate A260/A280 &gt;= 1.7, and RT-qPCR Ct values within 2 cycles of reference extraction. Zymo Research Quick-RNA Viral Kit remains the most frequently referenced spin-column format in WHO and CDC laboratory guidance documents for respiratory and bloodborne virus detection. RNase-free consumables (microcentrifuge tubes, tips, collection tubes) rated for spin-column use are required to prevent RNA degradation and column contamination during extraction.
<section class="mbp-contact-cta"></section>
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP Inc.</a> today and get high-sensitivity spin-column purification options for your lab.

MSDS (EU)

R1034-MSDS (US)

Protocol - R1034/R1035

Protocol - R1034-E/R1035-E

Quick-RNA Viral Kit

Quick-RNA™ Viral Kit by Zymo Research. Isolates viral RNA from plasma, serum, swabs, UTM/VTM, and other biological fluids. 10 µg binding capacity, ≥6 µl elution. Available in 50-prep (R1034) and 200-prep (R1035) formats, with CE-IVD DX versions (R1034-E, R1035-E).

Quick-RNA™ Viral Kit (50 Preps.)

Quick-RNA™ Viral Kit - DX (50 Preps.)

Quick-RNA™ Viral Kit (200 Preps.)

Quick-RNA Viral Kit (200 Preps.) -DX

<h2>What are FFPE RNA Extraction Kits?</h2>
&nbsp;
FFPE RNA extraction kits isolate total RNA from formalin-fixed paraffin-embedded tissue - the dominant archival format for surgical pathology specimens worldwide - using a multi-step protocol that removes paraffin, digests crosslinked proteins with proteinase K, applies heat to partially reverse formaldehyde-induced RNA crosslinks, and purifies RNA on a silica-membrane spin column. Formalin fixation crosslinks RNA to surrounding proteins through methylene bridges and fragments RNA to 100-300 bp; FFPE-specific kits are optimized for maximum recovery of this degraded, crosslinked RNA that standard tissue RNA kits cannot efficiently extract.&nbsp;Use an FFPE-specific kit whenever your starting material is a paraffin block or stained/unstained FFPE section - standard tissue RNA kits produce yields 5-20-fold lower from FFPE material than from fresh-frozen tissue.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="1ci26rv" data-start="0" data-end="131">Quick-RNA&trade; FFPE MiniPrep for purification of high-quality total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples.</li>
<li data-section-id="8rfh9e" data-start="132" data-end="246">Solvent-free deparaffinization workflows that eliminate the need for hazardous xylene or other organic reagents.</li>
<li data-section-id="j7jj92" data-start="247" data-end="364">Specialized heat-decrosslinking chemistry designed to reverse formalin-induced crosslinks and improve RNA recovery.</li>
<li data-section-id="1j7dh62" data-start="365" data-end="470">Spin-column purification technology that delivers clean RNA for reliable downstream molecular analysis.</li>
<li data-section-id="16nlq73" data-start="471" data-end="596">RNA extraction solutions optimized for archived tissue specimens used in biomarker, oncology, and gene expression research.</li>
<li data-section-id="17oavxo" data-start="597" data-end="710" data-is-last-node="">High-quality RNA preparation suitable for RT-PCR, qPCR, RNA sequencing, and other transcriptomics applications.</li>
</ul>
&nbsp;
<h2>How to Choose an FFPE RNA Extraction Kit</h2>
&nbsp;
Xylene-Free vs. Xylene-Based Deparaffinization
The Zymo Research Quick-RNA FFPE Miniprep Kit uses a proprietary Deparaffinization Solution that dissolves paraffin without xylene, eliminating fume-hood requirements for the deparaffinization step. QIAGEN RNeasy FFPE and AllPrep DNA/RNA FFPE Kits specify xylene or heptane as the primary deparaffinization solvent; both provide xylene-free alternatives in the protocol. Xylene-free workflows are preferred in high-throughput pathology labs where fume-hood access is a bottleneck.
RNA-Only vs. DNA/RNA Co-Extraction
For paired DNA sequencing and RNA expression profiling from the same archived specimen, the Zymo Quick-DNA/RNA FFPE Miniprep Kit (R1009) and QIAGEN AllPrep DNA/RNA FFPE Kit co-extract both analytes from the same tissue section, conserving limited archival material. For RNA-only applications (RT-qPCR, RNA-seq, NanoString), the Quick-RNA FFPE Miniprep Kit provides a simpler one-column workflow optimized for maximum RNA yield.
Section Thickness and Surface Area
Thinner sections (5-10 um) provide better proteinase K penetration and more complete crosslink reversal than thick sections (20 um). Use 2-4 sections per extraction as a starting point; tissue type and cellularity affect yield - highly cellular tumour sections yield more RNA per mg than fibrous stromal tissue. The Zymo Quick-RNA FFPE kit specifies a maximum of four sections &lt;=20 um with ~20 mm2 total surface area per extraction.
Amplicon Size for Downstream Assay
Before selecting a kit, confirm the expected RNA fragment size from your FFPE blocks. Blocks &lt;=5 years old typically yield RNA assayable at 150-200 bp amplicons; blocks &gt;10 years old may only reliably support amplicons of 60-100 bp. Select assays and library prep kits validated for fragmented FFPE RNA (e.g., Illumina TruSeq RNA Exome, Takara SMART-Seq FFPE).
DNase Treatment
Include on-column DNase I treatment for RT-qPCR and RNA-seq to remove gDNA contamination, which is particularly abundant in FFPE extracts because protein crosslinks trap gDNA in the same fraction as RNA. The Zymo Quick-RNA FFPE kit recommends optional on-column DNase I treatment; for sequencing applications, this step is mandatory.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
FFPE RNA quality expectations: RIN 1-4 for older blocks (&gt;5 years), 3-6 for recent blocks (&lt;3 years); A260/A280 &gt;= 1.8. RNA yield from 10 mg FFPE tissue is typically 100 ng to 2 ug depending on tissue type, block age, and fixation conditions. For RNA-seq from FFPE, short insert library preparation protocols tolerating fragmented input (fragment size 100-300 bp) are required; Illumina TruSeq RNA Exome and Takara SMART-Seq v4 FFPE are validated for FFPE RNA-seq at major cancer research centers. FFPE biobank samples from surgical pathology archives at institutions including MD Anderson Cancer Center and Vanderbilt University are used extensively in retrospective cancer transcriptomics studies, making reliable FFPE RNA extraction a clinical research priority. The Zymo Quick-RNA FFPE Miniprep is cited in published FFPE workflows for its xylene-free chemistry and compatibility with both standard RT-PCR and next-generation sequencing library preparation.
<section class="mbp-contact-cta"></section>
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.

FFPE

Quick-RNA™ FFPE MiniPrep (50 Preps.)

R1008 by Zymo Research is the Quick-RNA FFPE MiniPrep Kit (50 preps) for xylene-free total RNA extraction from FFPE, fresh, and frozen tissue. Up to 25 mg tissue input, ≥25 µL elution, ≤50 µg yield, A260/A280 and A260/A230 >1.8, with in-column DNase I treatment. Ready for RT-PCR and NGS.

United States

Texas

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