Name: Quick-cfRNA™ Serum and Plasma (50 Preps.)
SKU: R1059
Price: 405.65 USD
Availability: InStock

<h2>What are Cell-Free RNA Extraction Kits?</h2>
&nbsp;
Cell-free RNA (cfRNA) extraction kits isolate circulating RNA molecules - microRNAs, long non-coding RNAs, exosomal RNA, and mRNA fragments - from blood plasma, serum, urine, cerebrospinal fluid, and saliva, where endogenous RNA concentrations range from 1-100 ng/ml. Unlike standard total RNA kits designed for cell lysates containing micrograms of intracellular RNA per sample, cell-free RNA kits are built for the low-abundance, fragmented, and often vesicle-packaged RNA species present in acellular body fluids. They incorporate carrier RNA to prevent adsorption loss, handle input volumes of 200 ul to 5 mL, and use binding chemistries optimized for small RNAs &lt;200 nt. Use a cell-free RNA kit - not a standard total RNA or viral RNA kit - whenever your input is plasma, serum, urine, or another cell-depleted biofluid and your target is circulating miRNA, cfRNA, or exosomal RNA.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="1fsegxt" data-start="25" data-end="105">Rapid purification of circulating cell-free RNA (cfRNA) from plasma and serum.</li>
<li data-section-id="dahqiz" data-start="106" data-end="181">Efficient recovery of low-abundance cell-free RNA from biological fluids.</li>
<li data-section-id="85g4hb" data-start="182" data-end="245">Zymo-Spin&trade; spin-column technology for reliable RNA isolation.</li>
<li data-section-id="exy4np" data-start="246" data-end="296">Streamlined workflow for fast sample processing.</li>
<li data-section-id="mtq4uw" data-start="297" data-end="369" data-is-last-node="">Purified cfRNA suitable for downstream molecular biology applications.</li>
</ul>
&nbsp;
<h2>How to Choose a Cell-Free RNA Extraction Kit</h2>
&nbsp;
Input Volume and Throughput
Standard cell-free RNA kits accept 200 &mu;L-1 mL of plasma or serum per extraction; this input volume is appropriate for RT-qPCR quantification of abundant miRNA species and exosomal RNA panels. For discovery-phase cfRNA-seq, where rare transcripts are targets, use high-volume extraction formats to increase RNA mass from low-abundance circulating species.
Small RNA Enrichment
Circulating miRNAs are the most studied cfRNA analytes; kits that enrich for RNA &lt;200 nt deliver higher sensitivity for miRNA panels than kits recovering large and small RNAs together.&nbsp;
Carrier RNA and Spike-In Controls
Every cell-free RNA extraction should include both a carrier RNA (added to the lysis buffer to improve recovery) and an exogenous spike-in control RNA - typically Caenorhabditis elegans miR-39 (cel-miR-39) at 1.6 &times; 10^8 copies per extraction - added at the lysis step. Spike-ins normalize inter-sample variation in extraction efficiency and enable detection of failed extractions. Confirm whether carrier RNA is supplied in the kit or purchased separately.
Magnetic Bead vs. Spin-Column Format
Spin-column cfRNA kits (Zymo Quick-RNA Serum &amp; Plasma) are the standard for 1-24 samples per session and require only a microcentrifuge. Magnetic bead formats are preferred for higher-volume inputs and automation, and demonstrate superior recovery of fragmented low-abundance RNA from dilute matrices in published comparative studies.
Phenol-Free vs. Phenol-Containing Lysis
Most current cell-free RNA kits use guanidinium-based, phenol-free lysis buffers, avoiding the hazardous handling requirements of phenol-containing reagents. Phenol-chloroform-based extraction of cfRNA is used in research settings for the highest yield but introduces extraction bias for certain miRNA species and requires additional purification steps; column-based phenol-free kits are preferred for clinical and high-throughput biomarker applications.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Performance benchmarks for cell-free RNA extraction: recovery of cel-miR-39 spike-in within 2 Ct of the expected value, A260/A280 &gt;= 1.7 (lower values are acceptable for extremely dilute cfRNA samples), and consistent inter-sample Ct variation &lt;1.5 Ct for reference miRNAs across a batch. Circulating miRNA biomarkers -- including miR-21, miR-155, miR-210, miR-126, and miR-200 family members - are under clinical evaluation as non-invasive cancer, cardiovascular, and neurological disease biomarkers, with studies published by groups at institutions including MD Anderson Cancer Center and Vanderbilt University. Pre-analytical variables -- plasma preparation method, freeze-thaw cycles, hemolysis - are major sources of inter-study variability in cfRNA data; standardized two-spin plasma preparation protocols are recommended before extraction.
&nbsp;
For ordering assistance, bulk pricing, or protocol guidance on selecting the right kit for your sample type, <a href="https://mbpinc.net/contact-us">contact the MBP team</a>.&nbsp;

Cell-free RNA

MSDS

Protocol

White Paper

Quick-cfRNA™ Serum and Plasma (50 Preps.)

Quick-cfRNA™ Serum and Plasma Kit (R1059) by Zymo Research isolates cell-free RNA (≥17 nt, including microRNA) from up to 3 mL of plasma, serum, CSF, amniotic fluid, or urine in a spin-column workflow with 6–15 µl concentrated elution. No phenol-chloroform required. 50-prep format for liquid biopsy and cfRNA biomarker research.