Zymo Reagents

<h2>What Are 96-Well Plate Genomic DNA Kits for Blood and Tissue?</h2>
&nbsp;
96-well plate genomic DNA kits for blood and tissue replace individual spin columns with silica-binding or glass-fiber plates that enable simultaneous purification of genomic, mitochondrial, or pathogen DNA from up to 96 samples per run. These systems are designed for whole blood, serum, plasma, lymphocytes, cultured cells, and selected tissue samples, providing efficient high-throughput DNA extraction for research and molecular testing workflows.&nbsp;Choose a 96-well plate genomic DNA kit when sample volumes justify batch processing on a plate-compatible centrifuge or vacuum manifold.
&nbsp;
<h2>What You Will Find</h2>
&nbsp;
<ul>
<li>Streamlined high-throughput processing using 96-well plate formats for simultaneous genomic DNA purification.</li>
<li>High-purity DNA extraction from blood and tissue samples using optimized purification chemistries.</li>
<li>Compatibility with both automated and manual workflows for scalable sample preparation in research and diagnostic laboratories.</li>
<li>Purified DNA suitable for sequencing, PCR, qPCR, genotyping, and other downstream molecular biology applications.</li>
</ul>
<h2>&nbsp;</h2>
<h2>How to Choose a 96-Well Plate Blood and Tissue Genomic DNA Kit</h2>
&nbsp;
Sample Input and Well Capacity
Most kits accept up to 200 &micro;L of whole blood, serum, plasma, or approximately 5 &times; 106 cultured cells per well. Exceeding recommended input volumes can reduce DNA binding efficiency and overall recovery.
Centrifuge Rotor vs. Vacuum Manifold Compatibility
96-well plate kits typically require either a centrifuge equipped with a compatible 96-well plate rotor and deep bucket carriers or a vacuum manifold system. If your laboratory lacks a plate-compatible centrifuge, confirm vacuum manifold compatibility before selecting a kit.
Yield and Elution Volume
A typical extraction yields approximately 6 &micro;g of genomic DNA from 200 &micro;L of whole blood, with elution volumes commonly around 200 &micro;L using TE buffer or nuclease-free water. Processing times generally range from 45 minutes to a few hours, depending on workflow design.
Throughput and Sample Type Considerations
These kits are optimized for high-throughput processing and can handle large sample batches efficiently. When processing multiple sample types within the same plate, additional pretreatment steps may be required for certain tissues or cell types.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The key specifications for 96-well plate genomic DNA extraction kits include sample input volume, DNA yield, elution volume, and compatibility with centrifuge-based or vacuum-manifold workflows. Most kits in this category are designed for high-throughput purification of genomic DNA from blood, tissue, and cultured cell samples while maintaining consistent recovery across large sample batches.&nbsp;Products such as the Quick-DNA 96 Kit, Quick-DNA 96 Plus Kit, 96-well Blood DNA Kit, and 96-well Tissue DNA Kit support parallel processing in a plate-based format, helping laboratories scale routine genotyping, PCR, qPCR, and sequencing workflows. Typical workflows combine optimized lysis and silica-based DNA binding chemistry to remove inhibitors while producing purified DNA suitable for downstream molecular biology applications.&nbsp;When selecting a kit, confirm compatibility with your laboratory's centrifuge rotor, 96-well carrier, or vacuum manifold system, as equipment requirements vary between platforms. Most kit components are stored at room temperature, while any included enzyme reagents should be stored according to the manufacturer's recommendations. Lot-specific Certificates of Analysis (CoAs) are available upon request.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the MBP team </a>today to discuss high-throughput genomic DNA extraction workflows and receive expert guidance on selecting the right 96-well plate solution for your laboratory.

96-well plate

D4070-Data Sheet

D4070 - MSDS

D4070-Protocol

Quick-DNA 96 Plus Kit

D4070 by Zymo Research: Quick-DNA™ 96 Plus Kit, 2×96 preps. Extracts genomic DNA from biofluids, cells, and solid tissues. 5 µg/well binding capacity; ≥15 µl elution; Proteinase K digestion at 55°C. Typical yield: 5–7 µg from 100 µl whole blood. Made in USA.

Elution Volume

Equipment

Purity

Size Range

Workflow

Yield

Manufacturer

Shipping Condition

Storage Condition

Quick-DNA™ 96 Plus Kit (2 x 96 Preps.)

Quick-DNA™ 96 Plus Kit (4 x 96 Preps.)

<h2>What are microbial and environmental DNA isolation kits?</h2>
&nbsp;
Microbial and environmental DNA isolation kits purify DNA from soil, sediment, freshwater, marine water, feces, and other complex environmental matrices using lysis and purification chemistry designed to remove humic acids, metal compounds, and other inhibitors that interfere with PCR and sequencing, supporting 16S/18S rRNA amplicon profiling and shotgun metagenomic sequencing. Related entities include "kitome" and "splashome" contamination controls, alpha diversity and microbial community composition analysis, and compatibility with both short-read (Illumina) and long-read (Oxford Nanopore) sequencing platforms. Choose a kit validated for your specific sample matrix (soil, sediment, water, or feces), since benchmarking studies consistently show kit performance varies by matrix rather than one kit being universally best.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Spin-column fungal and bacterial kits for rapid DNA purification using rapid silica-membrane technology&nbsp;</li>
<li dir="ltr" role="presentation">Parallel extraction of soil, fecal, and environmental samples using high-throughput 96-well microplates&nbsp;</li>
<li dir="ltr" role="presentation">Automated&nbsp; purification of complex microbial and environmental specimens using Magnetic bead isolation systems</li>
<li dir="ltr" role="presentation">Scalable microprep and midiprep columns tailored for concentrated low-volume elution demands and diverse sample inputs</li>
</ul>
&nbsp;
<h2>How to choose a microbial and environmental DNA isolation kit</h2>
&nbsp;
Matrix-specific kit performance
Benchmarking studies comparing multiple commercial kits across sediment, water, soil, oyster gut microbiome, and mammalian feces have found that the best-performing kit varies by matrix - kits ranked highly for sediment did not necessarily perform best for water or host-associated samples. Rather than assuming a single "universal" kit, match your kit choice to published benchmarking results for your primary sample type where available.
Inhibitor removal for downstream PCR and sequencing
Environmental samples, especially sediments and soils, contain organic and metal compounds that inhibit PCR amplification if not removed during extraction; kits designed for these matrices integrate inhibitor-removal steps as part of the standard protocol. If your samples have historically produced low PCR amplification efficiency with general-purpose kits, an environmental-matrix-specific kit with built-in inhibitor removal is the first thing to evaluate.
Short-read vs. long-read sequencing compatibility
For long-read metagenomic sequencing, DNA fragment length and integrity from the extraction kit directly affect achievable read lengths, though longer extracted fragments don't always translate consistently into longer sequencing reads - kit choice affects both fragment length and downstream classification rate and reproducibility. If long-read metagenomics is your goal, prioritize kits with published long-read benchmarking data over kits validated only for short-read amplicon work.
Contamination control (kitome/splashome)
Kit-derived contamination ("kitome") and cross-sample contamination during processing ("splashome") can affect microbial community composition results, particularly for low-biomass samples; benchmarking studies evaluate kits specifically for these contamination sources alongside yield and diversity metrics. For low-biomass environmental samples, prioritize kits with documented low kitome contamination in published comparisons.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for microbial and environmental DNA isolation are matrix-specific validation (soil vs. sediment vs. water vs. feces), built-in inhibitor removal for PCR-ready output, and fragment length/integrity for long-read sequencing compatibility where relevant. Since benchmarking studies repeatedly show no single kit performs best across all matrices, identifying your primary sample type before selecting a kit is the most impactful decision. Labs running metagenomic surveys across multiple matrices (e.g., soil and water from the same field site) often need separate matrix-optimized kits rather than a single universal kit. The parent <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits hub</a>&nbsp;links to related sample-type categories, including&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/insect">insect genomic DNA kits</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/plant">plant genomic DNA kits</a>&nbsp;for organism-associated environmental work, and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/high-molecular-weight-dna">high molecular weight DNA kits</a>&nbsp;for long-read metagenomics requiring larger fragment sizes. For cell-free DNA in environmental water samples (eDNA detection), see&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/cell-free-dna">cell-free DNA kits</a>.&nbsp;
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Microbial & Environmental DNA Isolation

DataSheet

MSDS

Protocol

Quick-DNA Fecal/Soil Microbe MicroPrep Kit (50 Preps.)

D6012 Quick-DNA Fecal/Soil Microbe MicroPrep Kit by Zymo Research — 50 preps, ≤15 min, ≤50 mg feces or ≤250 mg soil input, inhibitor-free DNA, ≤5 µg yield, ≥20 µl elution.

Applicable For

Processing Volume

Sample Source

Sample Storage

Type

<h2>What Are Zymo Research Buffers?</h2>
Zymo Research buffers are formulation-specific reagents used at defined steps &mdash; lysis, binding, wash, and elution &mdash; in Zymo-Spin column-based purification protocols for DNA, RNA, and bisulfite-converted DNA. Each buffer is optimized for a specific kit family: DNA Wash Buffer concentrate (requires ethanol addition before use) removes salts and contaminants before elution from Zymo-Spin silica columns; DNA Elution Buffer (10 mM Tris, pH 8.5, 0.1 mM EDTA) releases purified DNA from the matrix; RNA Wash Buffer supports RNA extraction kits including Quick-RNA and Direct-zol lines; M-Elution Buffer is designed for eluting bisulfite-converted DNA in EZ DNA Methylation kits. Researchers typically order these separately when a single buffer component runs out before the rest of the kit, or when scaling up a protocol beyond the volume included.
TL;DR: Order Zymo buffers individually to replenish a specific step in your existing Zymo workflow without replacing the full kit.
<h2>How to Choose Zymo Research Buffers</h2>
<h3>Match the buffer to your kit family</h3>
Every Zymo buffer is paired to a specific kit line. DNA Wash Buffer and DNA/RNA Wash Buffer concentrate are used in DNA Clean &amp; Concentrator, Quick-DNA, and Zymoclean Gel DNA Recovery kits. RNA Wash Buffer pairs with Quick-RNA kits. ZymoBIOMICS DNA Wash Buffer 1 and Wash Buffer 2 are specific to the ZymoBIOMICS microbiomics line. Substituting a buffer from a different kit family can compromise yield or purity.
<h3>Confirm ethanol addition requirements before use</h3>
Several Zymo wash buffer concentrates require addition of 96-100% ethanol to the bottle before first use &mdash; the label states the exact volume. DNA Wash Buffer concentrate, DNA/RNA Wash Buffer concentrate, and Urine DNA Wash Buffer concentrate all require this step. Pre-made (non-concentrate) versions such as RNA Wash Buffer are supplied ready to use.
<h3>Select the right volume for your throughput</h3>
Zymo buffers are available in sizes from 6 ml (single-experiment replenishment) up to 500 ml (Buffer P1 Red, for high-volume plasmid prep labs). For labs processing more than 50 samples per week, ordering the largest available size reduces per-sample cost and the frequency of reordering.
<h3>Choose elution buffer chemistry based on downstream application</h3>
DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA) is the standard for most PCR and sequencing applications. For applications sensitive to EDTA &mdash; such as some restriction enzyme digests or direct use with divalent cation-dependent enzymes &mdash; eluting in molecular-grade water or a Tris-only buffer is preferable. M-Elution Buffer is specifically formulated for bisulfite-converted DNA and should not be substituted with standard elution buffer in methylation workflows.
<h3>Verify research-use-only status</h3>
All Zymo Research buffers sold through MBP are for research use only (RUO). They are not cleared for in vitro diagnostic use. For clinical or diagnostic laboratory applications, confirm regulatory status with your institutional compliance officer before use.
<h2>Compare Zymo Buffer Types</h2>
<table>
<thead>
<tr>
<th>Buffer Type</th>
<th>Primary Application</th>
<th>Kit Family</th>
<th>Format Available</th>
</tr>
</thead>
<tbody>
<tr>
<td>DNA Wash Buffer (concentrate)</td>
<td>Salt removal before DNA elution</td>
<td>DNA Clean &amp; Concentrator, Quick-DNA</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA/RNA Wash Buffer (concentrate)</td>
<td>Salt removal for dual DNA/RNA preps</td>
<td>Quick-DNA/RNA kits</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA)</td>
<td>DNA release from silica column</td>
<td>All Zymo DNA purification</td>
<td>16 ml &ndash; available sizes vary</td>
</tr>
<tr>
<td>RNA Wash Buffer</td>
<td>RNA purification wash step</td>
<td>Quick-RNA kits</td>
<td>Ready-to-use</td>
</tr>
<tr>
<td>M-Elution Buffer</td>
<td>Elution of bisulfite-converted DNA</td>
<td>EZ DNA Methylation kit families</td>
<td>Small volume</td>
</tr>
<tr>
<td>ZymoBIOMICS DNA Wash Buffer 2</td>
<td>Metagenomic/microbiomic DNA cleanup</td>
<td>ZymoBIOMICS DNA kits</td>
<td>Sold separately</td>
</tr>
<tr>
<td>Buffer P1 Red</td>
<td>Alkaline lysis resuspension (plasmid prep)</td>
<td>Zyppy Plasmid Miniprep</td>
<td>10 ml &ndash; 500 ml</td>
</tr>
</tbody>
</table>
<h2>Specifications Context</h2>
Zymo wash buffer concentrates are supplied at a defined fold-concentration (commonly 4x or 6x) and require ethanol addition to reach working strength &mdash; the exact ratio is printed on the bottle and in the kit protocol. Elution buffer volumes for Zymo-Spin columns typically range from 6 to 100 &micro;l per elution; larger elution volumes improve total yield at the cost of DNA concentration. For DNA elution from Zymo-Spin columns, the protocol recommends a minimum 1-minute contact time between elution buffer and the silica matrix to maximize recovery of fragments 10 kb and above. As of 2025, Zymo Research tests all buffer lots for performance consistency, with integrity guaranteed for up to one year from purchase date.
Browse the full <a href="https://www.notion.so/reagents">Zymo Research reagents collection at MBP</a> for kits and columns that use these buffers, or visit our <a href="https://www.notion.so/reagents/buffers">reagent buffers category</a> for non-Zymo buffer options including ABM and other brands. For volume orders or purchase orders, use the <a href="https://www.notion.so/quickorder">Quick Order</a> tool or <a href="https://www.notion.so/contact-us">contact us</a> directly &mdash; MBP is a registered vendor for institutional procurement systems.
Last updated: June 2025
&nbsp;

Zymo Buffers

D4077-1-MSDS

Quick-DNA MagBinding Buffer

D4077-1 by Zymo Research — Quick-DNA MagBinding Buffer, available in 150 mL and 250 mL. The binding buffer component for Zymo Research Quick-DNA MagBead DNA purification workflows, enabling DNA capture onto magnetic beads in automated and manual extraction protocols. Research Use Only.

Quick-DNA™ MagBinding Buffer (150 ml)

Quick-DNA™ MagBinding Buffer (250 ml)

<h2>What Are Spin-Column Genomic DNA Kits for Blood and Tissue?</h2>
&nbsp;
A spin-column genomic DNA kit for blood and tissue combines a proteinase K-based direct lysis step with a silica-membrane spin column to purify genomic, mitochondrial, or viral DNA from animal blood, tissue, and cultured cells without phenol, chloroform, or alcohol precipitation. Related entities include direct-lysis protocols, dried blood spot supplementary workflows, TissueLyser mechanical disruption, and downstream PCR, genotyping, and sequencing library preparation.&nbsp;Use spin-column formats for individual or small-batch genomic DNA extraction from blood, cells, or tissue using a standard microcentrifuge.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li>Efficient isolation of high-quality genomic DNA from blood, saliva, tissues, and other biological samples using rapid spin-column purification.&nbsp;</li>
<li>Maximize DNA recovery with optimized lysis and binding chemistry while minimizing inhibitor, protein, and contaminant interference</li>
<li>Midiprep, microprep, and miniprep formats available to support varied sample input volumes&nbsp;</li>
<li>User-friendly workflows and streamlined protocols with fast processing times for efficient genomic DNA extraction&nbsp;</li>
</ul>
&nbsp;
<h2>How to Choose a Spin-Column Blood/Tissue Genomic DNA Kit</h2>
&nbsp;
Lysis Approach by Sample Type
Whole blood, buffy coat, buccal cells, and cultured cells generally undergo direct proteinase K lysis without mechanical disruption. In contrast, fibrous or fatty tissues such as muscle, liver, or skin often require mechanical homogenization prior to the lysis step to ensure complete cell disruption.
Anticoagulant and Blood Sample Handling
These kits typically support fresh or frozen blood collected in EDTA, citrate, or heparin. Dried blood spots generally require supplementary protocols and additional equipment beyond standard spin-column workflows.
Column Elution Chemistry
Elution is typically performed using Tris-based buffer or nuclease-free water, with volumes ranging from approximately 50&ndash;200 &micro;L depending on kit design and sample type. Water elution may be preferred for EDTA-sensitive downstream applications.
Scaling Beyond a Single Column
For higher throughput workflows, 96-well plate formats using the same silica-membrane chemistry enable simultaneous processing of multiple samples per centrifuge run. Spin-column formats remain best suited for method development and low-to-moderate sample volumes.
&nbsp;
<h2>Specifications Context:</h2>
&nbsp;
The most important parameters for spin-column blood/tissue genomic DNA kits are sample-type compatibility and elution buffer composition, as these determine whether additional pretreatment is required and whether the eluate is directly compatible with downstream workflows. A typical 200 &micro;L whole blood input yields low-microgram quantities of genomic DNA in an elution volume of approximately 50&ndash;200 &micro;L.
&nbsp;
Use MBP's <a href="mbpinc.net/quickorder">Quick Order tool</a> for bulk pricing or <a href="mbpinc.net/contact-us">contact the specialist team</a> to confirm pretreatment requirements for challenging tissue types.

Spin-column

Quick-DNA Microprep Kit

C2010 by Zymo Research — 2-pack of Zymo-Spin™ I-96-XL deep-well 96-well purification plates for high-throughput HMW DNA (up to 5 µg/well) and RNA (up to 10 µg/well) purification. 1.1 ml well capacity; elution in ≥ 15 µl per well. Centrifuge-compatible. Pair with Air Permeable Sealing Cover (C2011-2).

Quick-DNA™ MicroPrep (50 Preps.)

Quick-DNA™ MicroPrep (200 Preps.)

Quick-DNA Miniprep

D3024 by Zymo Research is the Quick-DNA™ Miniprep Kit (50 preps) for rapid, Proteinase K-free gDNA isolation from whole blood (up to 200 µl), plasma, serum, cultured cells, and buccal samples—yielding 3–7 µg per 100 µl blood (up to 25 µg total) with A260/A280 &gt;1.8, DNA up to 40 kb+, in ≥50 µl eluate. The inhibitor-free output is directly compatible with PCR, qPCR, restriction digestion, and bisulfite conversion.

Quick-DNA™ MiniPrep (50 Preps.)

Quick-DNA™ MiniPrep (200 Preps.)

<h2>What Are 96-Well Plate Viral DNA Extraction Kits?</h2>
&nbsp;
A 96-well plate viral DNA extraction kit replaces individual spin columns with a silica-membrane plate, allowing up to 96 cell-free liquid samples to be lysed, bound, washed, and eluted simultaneously using either a centrifuge equipped with a 96-well rotor carrier or a vacuum manifold. These systems support high-throughput viral DNA purification workflows while maintaining the nucleic acid quality required for PCR, qPCR, sequencing, and molecular diagnostics.&nbsp;Choose a 96-well plate viral DNA extraction kit when sample volumes justify batch processing, and your laboratory has compatible centrifuge or vacuum-manifold equipment.
&nbsp;
<h2>What you will find:&nbsp;</h2>
&nbsp;
<ul>
<li>Quick-DNA Viral 96 Kit: Enables efficient parallel processing of up to 96 samples in a single workflow.</li>
<li>Sequencing Ready: Produces purified viral nucleic acids suitable for next-generation sequencing applications.</li>
<li>PCR &amp; qPCR Compatible: Delivers high-quality DNA and RNA for PCR, qPCR, and RT-qPCR workflows.</li>
<li>Molecular Diagnostics Support: Ideal for high-throughput viral screening and diagnostic research applications.</li>
</ul>
<h2>&nbsp;</h2>
<h2>How to Choose a 96-Well Plate Viral DNA Extraction Kit</h2>
&nbsp;
Centrifuge Rotor and Minimum Speed Requirements
Most 96-well plate kits require a centrifuge equipped with a compatible 96-well plate rotor or carrier. Some systems specify minimum centrifugal forces around 2,250 &times; g and sufficient bucket depth to accommodate the plate format. Confirm equipment compatibility before selecting a kit.
Vacuum Manifold Compatibility
Many plate-based viral DNA extraction kits can also be operated using a vacuum manifold instead of centrifugation. This option is particularly useful for laboratories already utilizing vacuum-based nucleic acid purification workflows.
Elution Volume and DNA Concentration
Some kits support elution volumes as low as 6&ndash;10 &micro;L of DNase/RNase-free water, enabling recovery of highly concentrated viral DNA suitable for low-titer samples and sensitive qPCR applications. Larger elution volumes may be preferable when sample concentration is less critical, and ease of handling is a priority.
Throughput and Workflow Efficiency
While plate-format kits generally have a higher upfront cost than individual spin-column kits, they significantly reduce hands-on time per sample when processing large batches. Laboratories routinely processing dozens of samples per run typically benefit most from plate-based workflows.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The most important specifications for 96-well plate viral DNA extraction kits are sample input capacity, minimum elution volume, and equipment compatibility requirements. Many systems process up to 400 &micro;L of sample per well and support low-volume elution for concentrated DNA recovery.&nbsp;Plate-based workflows are commonly adopted by laboratories transitioning from single-column extraction methods to higher-throughput operations. By combining silica-membrane purification with automation-friendly formats, these kits help standardize viral DNA extraction across large sample sets while maintaining consistent performance for downstream PCR, qPCR, and sequencing applications.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> today to discuss your high-throughput viral DNA extraction requirements and receive expert guidance on selecting the right 96-well plate solution for your laboratory workflow.

Quick-DNA Viral 96 Kit

D3017 by Zymo Research is a 96-well viral DNA isolation kit providing 2 × 96 preps. Accepts ≤200 µl whole blood, plasma, serum, tissue, or cultured cells; elutes in ≥10 µl with A260/A280 ≥1.8 and DNA spanning 100 bp–50 kb. Compatible with qPCR, NGS, PCR, Southern blotting, and restriction digestion.

Quick-DNA™ Viral 96 Kit (2 x 96 Preps.)

Quick-DNA™ Viral 96 Kit (4 x 96 Preps.)

<h2>What are Magnetic Bead Microbial DNA/RNA Co-Extraction Kits?</h2>
&nbsp;
Magnetic bead microbial DNA/RNA co-extraction kits purify both genomic DNA and total RNA, including small RNAs &gt;=17 nt, from microbial community samples - stool, soil, water, biofilm, swabs, and body fluids using BashingBead mechanical lysis in a 96-deep-well plate followed by binding of nucleic acids to paramagnetic silica-coated beads, magnetic separation during successive wash steps to remove PCR inhibitors, and elution in &gt;=50 ul of ZymoBIOMICS DNase/RNase-Free Water. The entire purification phase requires no centrifugation and integrates directly with KingFisher Flex, Accuris IsoPure 96, and AllSheng AutoPure 96 liquid-handling platforms. Choose the magnetic bead format for microbial DNA/RNA co-extraction when processing 48+ community samples per session, when a compatible automation platform is available, or when reproducibility across hundreds of samples is required for a human microbiome cohort, environmental monitoring, or clinical trial.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Quick-DNA/RNA HT Kit for high-throughput isolation of total nucleic acids from liquid clinical and biological specimens using a magnetic bead&ndash;based extraction system.
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
ZymoBIOMICS MagBead DNA/RNA Kit for unbiased recovery of microbiome DNA and RNA without chemical bias, featuring an innovative lysis system.
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Quick-DNA/RNA Pathogen MagBead Kit for rapid isolation of viral, bacterial, and protozoan nucleic acids from challenging vector samples using an optimized buffer
</li>
</ul>
&nbsp;
<h2>How to Choose a Magnetic Bead Microbial DNA/RNA Co-Extraction Kit</h2>
&nbsp;
Automation Script Availability
Confirm the kit manufacturer provides a validated, instrument-specific script for your platform before purchase. Zymo Research provides automation scripts for KingFisher Flex, KingFisher Apex, Accuris IsoPure 96, and AllSheng AutoPure 96; Tecan Fluent and Hamilton STAR scripts require adaptation. Script development and validation add 2-4 weeks; factor this into project timelines.
Combined vs. Separate Analyte Fractions
The ZymoBIOMICS MagBead DNA/RNA kit elutes a combined total nucleic acid fraction. For studies requiring separate DNA and RNA fractions from the same extraction (parallel metagenomics and metatranscriptomics), apply post-extraction enzymatic depletion (DNase I on the RNA aliquot, RNase A on the DNA aliquot) or use the ZymoBIOMICS DNA/RNA Miniprep kit, which uses column-switching architecture for native separation.
96-Well Bead-Beating Setup
The lysis step requires a 96-well bead mill compatible with ZR-96 BashingBead Lysis Racks. Validated instruments include GenoGrinder 2000, Retsch MM400 with a 96-tube rack adapter, and Bertin Precellys. The bead-beating step is the primary manual step in an otherwise automated workflow; confirm the lab has a compatible bead mill before adopting the kit.
Inhibitor Load by Sample Type
Stool samples have moderate inhibitor loads (bile salts, starch metabolites) that ZymoBIOMICS wash steps reliably remove. Soil samples -- especially high-humic tropical or peat soils - carry heavier inhibitor loads that may require reducing input mass to 10-50 mg per well and validating A260/A230 &gt;= 1.5 on initial test extractions. If A260/A230 remains below 1.5, apply an additional cleanup step with Zymo OneStep PCR Inhibitor Removal Columns before library preparation.
DNA/RNA Shield Sample Collection
For multi-site human microbiome cohort studies, use DNA/RNA Shield fecal collection tubes (Zymo R1101) as the collection device. Samples stabilized at room temperature for up to 30 days in Shield are directly loadable into the ZR-96 BashingBead Rack, streamlining the pre-extraction workflow and enabling consistent RNA preservation across diverse collection sites.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
ZymoBIOMICS MagBead DNA/RNA kit produces total nucleic acid eluted into &gt;=50 ul with A260/A280 &gt;= 1.8, A260/A230 &gt;= 1.5, and DNA fragment sizes suitable for 16S rRNA amplicon sequencing and shotgun metagenomics. Input specification: approximately 2 &times; 10^8 bacterial cells, 2 &times; 10^7 yeast cells, or ~40 mg human stool per well. A published 2024 high-throughput fecal microbiome DNA extraction study demonstrated that bead-beating at &gt;=3,000xg significantly improves detection of hard-to-lyse Gram-positive genera - Blautia, Bifidobacterium, and Ruminococcus compared with no bead-beating, confirming that mechanical lysis is non-negotiable for unbiased community co-extraction. ZymoBIOMICS MagBead workflows are used in NIH-funded human microbiome research and are the standard extraction approach for multi-site gut microbiome cohort studies at institutions registered with MBP, including Howard Hughes Medical Institute and MD Anderson Cancer Center.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> and get high-yield mag bead systems for your high-throughput extraction workflows.

Mag Beads

MSDS (R2150-E/R2151-E)

MSDS (R2150/R2151)

R2152-MSDS

Protocol (R2150/R2151)

Protocol (R2150-E/R2151)

R2152-Protocol

Quick-DNA/RNA HT

R2150 by Zymo Research is the Quick-DNA/RNA&#8482; HT kit (250 preps), a magnetic bead-based system for simultaneous co-purification of total DNA and RNA from swabs, blood, plasma, serum, saliva, tissue, and samples in transport media (UTM, VTM, DNA/RNA Shield, PAXgene). Binding capacity of 5 µg per 10 µl HT MagBinding Beads, elution in ≥15 µl, with no centrifugation required — fully compatible with automated platforms. CE-IVD version (R2150-E) available for regulated workflows.

Binding Capacity

Sample Sources

Storage Temperature and Stability

Quick-DNA/RNA™ HT (250 preps)

Quick-DNA/RNA™ HT - Dx (250 preps)

Quick-DNA/RNA™ HT (1000 preps)

Quick-DNA/RNA™ HT - Dx (1000 preps)

<h2>What are Spin-Column DNA/RNA Co-Extraction Kits for Cells, Tissue, and Blood?</h2>
&nbsp;
Spin-column DNA/RNA co-extraction kits for cells, tissue, and blood simultaneously purify genomic DNA and total RNA in separate fractions from the same mammalian sample using a sequential two-column silica-membrane workflow in a standard microcentrifuge. The Zymo Quick-DNA/RNA Miniprep Plus Kit loads the sample lysate onto a Zymo-Spin IIICG column that physically captures gDNA while RNA passes through to a Zymo-Spin IIC column for binding, washing, and elution - completing both DNA and RNA purification in under 15 minutes for cell pellets and 45-75 minutes (including Proteinase K digestion) for solid tissue biopsies. Choose the spin-column format for DNA/RNA co-extraction from cells, tissue, and blood when processing 1-24 samples per run, when automation infrastructure is not available, or when the fastest possible DNA + RNA turnaround from a limited biopsy sample is required.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Quick-DNA/RNA&trade; Miniprep Kit for rapid co-extraction of total DNA and RNA from blood and tissues utilizing advanced spin-column technology
</li>
<li dir="ltr" aria-level="1">
Quick-DNA/RNA&trade; Microprep Plus Kit to recover high-purity nucleic acids from extremely small or rare samples using a specialized micro-elution column
</li>
<li dir="ltr" aria-level="1">
Quick-DNA/RNA Miniprep Plus Kit to ensure high-yield genomic DNA and total RNA yields using an ultra-clean spin-column workflow
</li>
<li dir="ltr" aria-level="1">
Blood RNA Mini Kit for the specific extraction of intact viral and total host RNA from whole blood samples using robust silica-membrane columns
</li>
</ul>
&nbsp;
<h2>How to Choose a Spin-Column DNA/RNA Co-Extraction Kit for Cells, Tissue, and Blood</h2>
&nbsp;
Miniprep Plus vs. Microprep Plus Format
The Miniprep Plus kit handles standard inputs (up to 5x10^6 cells or 30 mg tissue) and elutes in 25-100 ul per fraction, appropriate for most downstream applications. The Microprep Plus kit is designed for very low inputs -- 500 cells to 5,000 cells or 0.5-5 mg tissue -- with minimum elution volumes of 6-10 ul per fraction for maximum concentration from limited material. Use Microprep Plus for needle biopsy cores, laser-capture microdissected cells, single-well organoid lysates, and sorted immune cell populations.
DNA/RNA Shield for Sample Lysis and Stabilization
DNA/RNA Shield in the Miniprep Plus and Microprep Plus kits performs dual duty: it lyses cells on contact while simultaneously stabilizing DNA and RNA at room temperature, enabling sample collection and processing to be decoupled by hours or days without cold-chain handling. For tissue biopsies collected in the operating room that cannot be processed immediately, submerging in DNA/RNA Shield preserves RNA integrity for up to 30 days at room temperature -- the practical alternative to snap-freezing in liquid nitrogen.
Tissue Digestion Strategy
Soft, high-cellularity tissues (liver, spleen, kidney cortex) lyse completely in DNA/RNA Shield + Proteinase K at 55 degrees C within 30 minutes. Fibrous tissues (skeletal muscle, heart, skin, lung) require 1-2 hours of Proteinase K digestion or mechanical homogenization (bead-beating, tissue grinder) before column loading. For very fibrous tissue that resists Proteinase K digestion, add bead-beating in a lysis tube (0.5 mm beads) with DNA/RNA Lysis Buffer before proceeding to column loading.
On-Column DNase I for the RNA Fraction
Always include on-column DNase I treatment for RNA-seq and for RT-qPCR assays without intron-spanning primers. Skip DNase I only for RT-qPCR with exon-spanning primer pairs that distinguish cDNA from gDNA amplicons. The IIICG column physically depletes the majority of gDNA but does not eliminate 100%; residual gDNA in the RNA fraction produces false-positive amplification in RT-qPCR assays without an intron-spanning design.
Elution Strategy for Downstream Applications
For RNA-seq: elute RNA in 50-100 ul, quantify by Qubit RNA HS Assay, and verify RIN &gt;= 7 on a Bioanalyzer before library preparation. For RT-qPCR: elute in 25-50 ul for higher RNA concentration. For gDNA applications requiring &gt;= 5 kb fragments (WGS, WES, long-read sequencing): elute DNA in 50-100 ul and quantify by Qubit dsDNA BR Assay; avoid vortexing gDNA eluates to preserve high-molecular-weight fragment integrity.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The Zymo Quick-DNA/RNA Miniprep Plus Kit produces gDNA (A260/A280 &gt;= 1.8, high molecular weight &gt;= 20 kb) and total RNA, including small RNAs &gt;=17 nt (A260/A280 &gt;= 1.8, RIN &gt;= 7 from fresh tissue) in under 15 minutes from cell pellets, with both analytes ready for next-generation sequencing without further cleanup. The Quick-DNA/RNA Miniprep Plus Kit is among the most widely used spin-column co-extraction kits in academic molecular biology, gene therapy, pharmacology, and cancer multi-omics research; its use is cited in published studies at institutions including Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center for paired genomic and transcriptomic analyses. For needle biopsy or LCM inputs where the Miniprep Plus minimum input cannot be met, the Microprep Plus format recovers both analytes from the single-cell level without carrier RNA or pre-amplification requirements.
&nbsp;
For guidance on selecting the right spin-column co-extraction format for your input type or for pricing, <a href="https://mbpinc.net/contact-us">contact the expert team at MBP Inc. </a>

MSDS - D7003

MSDS - D7003T

Quick-DNA/RNA Miniprep Plus Kit

The Quick-DNA/RNA Miniprep Plus Kit (D7003) by Zymo Research isolates both high-molecular-weight DNA (≥40 kb) and total RNA including miRNA (≥17 nt) simultaneously from a single sample, supporting FFPE, blood, cells, and tissue in 50 preps with DNase I, Proteinase K, and DNA/RNA Shield included.

Quick-DNA/RNA MiniPrep Plus Kit (50 Preps.)

Quick-DNA/RNA™ MiniPrep Plus Kit (10 Preps.)

R2145-Protocol

Quick-DNA/RNA Pathogen MagBead

Quick-DNA/RNA Pathogen MagBead by Zymo Research — magnetic bead co-purification of DNA and RNA from insect vectors (mosquitoes, ticks, fleas), tissue, and biological liquids. Binding capacity 10 µg/20 µl beads; ≥50 µl elution. Available as R2145 (96 preps) and R2146 (4×96 preps).

Equipment Needed

Materials

Sample Type

Size

Quick-DNA/RNA™ Pathogen MagBead (96 Preps.)

Quick-DNA/RNA™ Pathogen MagBead (4 x 96 Preps.)

<h2>What are 96-Well Plate Viral DNA/RNA Co-Extraction Kits?</h2>
&nbsp;
96-well plate viral DNA/RNA co-extraction kits purify both viral DNA and RNA from up to 96 cell-free samples simultaneously - plasma, serum, urine, nasopharyngeal swabs, saliva, CSF, and cell culture media -- using the same single-buffer lysis chemistry as spin-column viral kits but operating in a Zymo-Spin I-96 spin plate processed with a 96-well plate centrifuge or vacuum manifold. Both analytes co-elute per well in DNase/RNase-free water covering RNA viruses (SARS-CoV-2, influenza, HIV) and DNA viruses (adenovirus, HPV, parvovirus) without requiring robotic automation. The format provides a significant throughput step-up from single-tube spin columns - processing 96 samples in under 30 minutes with a multichannel pipette and standard centrifuge. Choose the 96-well plate format when daily viral sample volumes exceed 24 per run, when automation infrastructure is unavailable, or when batch surveillance testing requires rapid plate-scale turnaround from clinical specimens.
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<h2 data-start="0" data-end="23">What you will find:</h2>
&nbsp;
<ul>
<li>Quick-DNA/RNA Viral 96 Kit (D7022)&nbsp;for concurrent isolation of viral DNA and viral RNA from fluid specimens.</li>
<li>96-well plate format for high-throughput sample processing.</li>
<li>Rapid purification workflow for efficient nucleic acid recovery.</li>
<li>Designed for processing multiple samples simultaneously.</li>
<li>Purified viral nucleic acids suitable for downstream molecular biology applications.</li>
</ul>
</div>
</div>
</div>
</div>
</div>
</div>
</section>
</div>
&nbsp;
<h2>How to Choose a 96-Well Plate Viral DNA/RNA Co-Extraction Kit</h2>
&nbsp;
Centrifuge vs. Vacuum Manifold Operation
A 96-well plate centrifuge rotor (accommodating the Zymo-Spin I-96 Plate dimensions) is the standard equipment requirement. If a compatible plate centrifuge is unavailable, a vacuum manifold that fits 96-well spin plates provides equivalent performance and eliminates centrifuge dependency. Confirm equipment compatibility before kit purchase.
Manual vs. Semi-Automated Pipetting
The 96-well format is designed for manual operation with multichannel pipettes for buffer addition and sample loading. Semi-automation is achievable with a liquid handler for buffer dispensing steps, leaving centrifugation as the manual step. For full automation without centrifugation, use the Quick-DNA/RNA Viral MagBead format on a compatible robot.
Lysis Buffer Volume Per Well
The single-buffer lysis system adds 200 ul of lysis buffer per well of sample (1:1 v/v for 200 ul input). Ensure the plate's well volume accommodates the combined lysis buffer + sample volume (400 ul total per well) plus ethanol addition before loading. Most Zymo-Spin I-96 plate protocols include a deep-well collection plate for this mixing step.
Positive and Negative Control Layout
Reserve wells 11 and 12 of each row for extraction controls. Fixed control placement across all plates enables automated data review scripts to flag failed wells based on consistent control positions.
Throughput Scaling Beyond 96
For programs processing 192-384 samples per day, run two 96-well plates sequentially on the same centrifuge or vacuum manifold. For volumes above 384 per day, transition to the magnetic bead format on a liquid handler (KingFisher, Tecan) to process 96 samples per automated run without per-batch manual centrifugation steps.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The Quick-DNA/RNA Viral 96 Kit employs a single buffer system that facilitates viral particle lysis and DNA/RNA adsorption onto the Zymo-Spin I-96 Plate matrix, with validated recovery of enteroviruses, rhinoviruses, coronaviruses, HIV, HCV, influenza A, flaviviruses, measles, parainfluenza, and parvovirus. Nucleic acids are eluted in DNase/RNase-free water suitable for RT-PCR, NGS, and hybridization applications. 96-well plate viral extraction is the standard format in public health and reference laboratory surveillance programs, processing 96-384 clinical specimens per day, including respiratory multiplex panels, arboviral surveillance, and bloodborne pathogen screening, with throughput requirements driving adoption of this format over single-tube spin columns.
&nbsp;
For bulk pricing on Quick-DNA/RNA Viral 96 kits, centrifuge compatibility checks, or guidance on pairing with validated multiplex RT-qPCR assays,&nbsp;<a href="https://mbpinc.net/contact-us">contact the MBP team</a>.&nbsp;

MSDS - D7022

D7023

Quick-DNA/RNA Viral 96 Kit

The Quick-DNA/RNA Viral 96 Kit (D7022) by Zymo Research simultaneously recovers viral DNA and RNA in a 96-well format from plasma, serum, saliva, urine, blood, and other samples — yielding up to 5 µg DNA and 10 µg RNA per prep in DNase/RNase-free water. No organic denaturants or proteases; eluate is directly compatible with RT-PCR, qPCR, and NGS.

Quick-DNA/RNA™ Viral 96 Kit (2 x 96)

Quick-DNA/RNA™ Viral 96 Kit (4 x 96 Preps.)

<h2>What are Magnetic Bead Viral DNA/RNA Co-Extraction Kits?</h2>
&nbsp;
Magnetic bead viral DNA/RNA co-extraction kits purify both viral DNA and RNA from plasma, serum, urine, nasopharyngeal swabs, saliva, blood, cellular suspensions, biopsies, fecal samples, and cell culture media stored in DNA/RNA Shield using paramagnetic silica-coated beads that bind nucleic acids from &gt;50 nt to &gt;200 kb during a single chaotropic lysis step, with magnetic separation during successive wash steps removing inhibitors - no centrifugation required during purification. The 96-well format integrates with KingFisher Flex, Tecan Fluent, and Hamilton STAR liquid-handling platforms, enabling fully automated extraction of both RNA viruses (SARS-CoV-2, influenza, HIV) and DNA viruses (adenovirus, herpesvirus, parvovirus) from 96 samples per run. Choose the magnetic bead format for viral DNA/RNA co-extraction when processing 48+ samples per run, when a compatible automation platform is available, or when the program requires EUA-associated workflows validated for SARS-CoV-2 or other respiratory panel targets.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Magbio HighPrep Viral-Bacterial DNA &amp; RNA Kit (HVB-DR0): Scalable magnetic bead chemistry optimized for the simultaneous extraction of viral and bacterial nucleic acids from diverse fluid profiles.
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Magbio HighPrep Viral DNA &amp; RNA Kit (HPV-DR0): High-efficiency magnetic isolation systems engineered for the rapid purification of viral target vectors from fluid specimens.
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Magbio HighPrep Viral-Pathogen DNA &amp; RNA Kit (HPP-DR0): Specialized bead technology designed to maximize pathogen recovery and eliminate inhibitors from challenging biological matrices.
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Quick-DNA/RNA Viral MagBead (R2140): Advanced magnetic-bead separation configurations built for fast, high-purity co-purification of total viral nucleic acids.
</li>
</ul>
&nbsp;
<h2>How to Choose a Magnetic Bead Viral DNA/RNA Co-Extraction Kit</h2>
&nbsp;
Automation Platform Compatibility
Confirm the kit's automation scripts are available for your specific platform before purchase. The Quick-DNA/RNA Viral MagBead Kit is used in Zymo Research's SARS-CoV-2 EUA workflow and is compatible with KingFisher and other major liquid-handling platforms; contact MBP's technical team or Zymo Research's automation support for current script availability. For Tecan Fluent or Hamilton STAR, platform-specific script development or adaptation may be required.
DNA/RNA Shield as the Collection Medium
The Quick-DNA/RNA Viral MagBead Kit is designed around DNA/RNA Shield as the sample collection, inactivation, and storage medium. Swabs or other samples stabilized in DNA/RNA Shield load directly into the extraction protocol without additional neutralization steps. For programs using VTM (viral transport medium), UTM, or PBS as collection media, confirm compatibility with the kit's lysis buffer by testing spike-in recovery before adopting at scale.
Carrier RNA Requirement
Unlike the QIAGEN QIAamp Viral RNA Mini Kit, the Quick-DNA/RNA Viral MagBead Kit does not require the user to prepare and add carrier RNA to the lysis buffer - the kit's buffer system is formulated for complete lysis and efficient nucleic acid binding without this step. Eliminating the carrier RNA preparation step reduces per-batch setup time and removes one source of inter-batch variability in high-throughput automated workflows.
Nucleic Acid Size Recovery Range
Confirm the kit recovers the size range needed for your downstream application. The Quick-DNA/RNA Viral MagBead Kit binds nucleic acids from &gt;50 nt to &gt;200 kb - covering small viral RNAs (miRNA-sized fragments from viral genomes), standard RT-qPCR amplicon targets, and full-length viral genomes for metagenomic NGS. Applications requiring recovery of very short fragments (&lt;50 nt) need a kit specifically validated for that range.
Regulatory Requirements
For IVD diagnostic use or regulatory submissions, confirm the kit's current regulatory status. The Quick-DNA/RNA Viral MagBead Kit has been used in EUA workflows; its current authorizations and intended uses may have evolved since initial authorization. Always verify directly with Zymo Research or MBP's regulatory affairs team for current compliance documentation.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The Quick-DNA/RNA Viral MagBead Kit binds small (&gt;50 nt) and large (&gt;200 kb) DNA and RNA to magnetic beads and elutes high-quality nucleic acids ready for NGS, hybridization-based detection, and RT-qPCR. The kit is used in Zymo Research's SARS-CoV-2 EUA extraction workflow and has been adopted in published multiplex respiratory panel methods and pan-pathogen metagenomics protocols. A published PMC study on integrated DNA and RNA extraction from viral pathogens causing acute respiratory infections demonstrated that magnetic bead-based co-extraction achieved equivalent or superior sensitivity to spin-column formats for both RSV (RNA virus) and adenovirus (DNA virus) from the same nasopharyngeal swab input. Magnetic bead viral co-extraction is the standard format for large public health reference laboratories and clinical metagenomics programs processing 96-1,500+ samples per day across outbreak response and surveillance networks.
&nbsp;
Contact the helpful <a href="https://mbpinc.net/contact-us">team at MBP</a> today to request a quote for your specific research requirements.

R2140-MSDS

R2140-Protocol

Quick-DNA/RNA Viral MagBead

R2140 by Zymo Research is the Quick-DNA/RNA&trade; Viral MagBead kit, a 250-prep magnetic bead system that simultaneously isolates DNA and RNA from up to 200 &micro;l of plasma, serum, urine, saliva, whole blood, cell culture media, or swab samples. Elution volume is 30 &micro;l (standard); binding capacity is 5 &micro;g DNA/RNA per 10 &micro;l of MagBinding Beads. Size range is 50 nucleotides to ~200 kb. All steps run at room temperature.
Compatible with KingFisher, Hamilton, and Tecan automated liquid-handling platforms. A CE-IVD version (R2140-E) is available. Related: R2141 (1,000 preps).
MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, Eurofins, and many other institutes across the USA. Order via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or contact <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.

Quick-DNA/RNA Viral MagBead (250 Preps.)

Quick-DNA/RNA™ Viral MagBead - DX (250 Preps.)

Quick-DNA/RNA Viral MagBead (1000 Preps.)

Quick-DNA/RNA Viral MagBead - DX (1000 Preps)

Load N’ Go™ DNA/RNA Viral Kit

<h2>What are FFPE DNA/RNA Co-Extraction Kits?</h2>
&nbsp;
FFPE DNA/RNA co-extraction kits simultaneously isolate genomic DNA and total RNA -- including small RNAs &gt;=17 nt from the same formalin-fixed paraffin-embedded tissue section using xylene-free deparaffinization, Proteinase K digestion, heat-mediated formaldehyde crosslink reversal, and Zymo-Spin column purification. Co-extraction from a single undivided FFPE section conserves precious archival material and ensures both DNA (for somatic mutation calling, CNV analysis, methylation profiling) and RNA (for expression profiling, fusion detection, nCounter panels) originate from identical tissue, eliminating the spatial heterogeneity introduced when different tissue sections or block portions are used for each analyte. Use an FFPE DNA/RNA co-extraction kit whenever archival tissue is limited, as in needle biopsy cores, small surgical biopsies, or rare tumour specimens, or when integrated multi-omic analysis (paired WES + RNA-seq, or mutation + expression correlation) is required from the same pathological specimen.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Quick-DNA/RNA&trade; FFPE MiniPreps: Efficient systems designed to extract high-quality total nucleic acids with reduced sequence bias, tailored for subsequent qPCR and NGS library preparation.
</li>
<li dir="ltr" aria-level="1">
Decross-linking Agents: Nuclease-free solutions formulated to effectively liberate nucleic acids from protein complexes, ensuring transfection-grade purity for delicate bioinformatics workflows.
</li>
</ul>
&nbsp;
<h2>How to Choose an FFPE DNA/RNA Co-Extraction Kit</h2>
&nbsp;
Xylene-Free vs. Xylene-Based Deparaffinization
Zymo Research Quick-DNA/RNA FFPE Miniprep Kit uses a proprietary Deparaffinization Solution that removes paraffin without xylene, making it safer for labs without fume hoods and eliminating toxic waste disposal requirements.
Tissue Section vs. Tissue Block Curling
For formalin-fixed paraffin-embedded sections on slides, cut and scrape sections directly into the Deparaffinization Solution tube - 2-4 sections of 5-10 um thickness is the standard starting point. For tissue blocks, cut 10-20 um sections with a microtome into a 1.5 ml tube. Avoid over-cutting; excessive paraffin volume from thick sections delays deparaffinization and reduces proteinase K efficiency, leading to lower DNA and RNA yields.
DNA and RNA Fraction Separation
The Zymo Quick-DNA/RNA FFPE Miniprep Kit delivers non-crosslinked DNA and DNA-free total RNA in separate fractions, making it suitable for downstream molecular analyses from the same FFPE sample.
Amplicon Size for Downstream Assays
RNA isolated from FFPE tissue is typically fragmented because of formalin fixation. For RT-qPCR, primer pairs producing amplicons of approximately 70&ndash;150 bp generally provide the most reliable amplification. The purified RNA from the Quick-DNA/RNA&trade; FFPE MiniPrep is suitable for downstream applications including RT-PCR, next-generation sequencing (NGS), microarray analysis, and hybridization, provided library preparation and assay workflows are compatible with fragmented FFPE-derived nucleic acids.
Block Age Assessment
Request pathology records for fixation time and block age before extraction. For blocks &gt;10 years old, perform a diagnostic RT-qPCR on a short amplicon (60-80 bp) reference gene in the RNA fraction before committing to full RNA-seq library preparation. RNA from blocks with Ct &gt;35 for abundant reference genes (ACTB, GAPDH) at short amplicons typically fails RNA-seq quality thresholds.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The Zymo Quick-DNA/RNA&trade; FFPE Miniprep Kit delivers non-crosslinked DNA and DNA-free total RNA (including small RNAs &ge;17 nt) from FFPE tissue using a xylene-free deparaffinization workflow. The purified nucleic acids are suitable for downstream applications including PCR, RT-PCR, next-generation sequencing (NGS), hybridization, and other molecular analyses compatible with FFPE-derived samples. Because formalin fixation fragments nucleic acids, downstream assays are generally designed with short amplicons (approximately 60&ndash;150 bp for RNA and 100&ndash;300 bp for DNA) to improve amplification efficiency. Co-isolation of DNA and RNA from the same FFPE specimen supports integrated genomic and transcriptomic analyses while preserving limited archival tissue.
&nbsp;
For FFPE kit selection, bulk pricing on Zymo Quick-DNA/RNA FFPE Miniprep kits, or protocol guidance for aged or low-cellularity archival blocks,&nbsp;<a href="https://mbpinc.net/contact-us">contact the MBP team</a>.&nbsp;

FFPE

Quick-DNA/RNA™ FFPE MiniPrep (50 Preps.)

Quick-DNA/RNA FFPE MiniPrep R1009 by Zymo Research: 50-prep kit for simultaneous DNA and RNA isolation from FFPE tissue. No xylene required; 50 µg binding capacity; A260/A280 ≥1.8; total RNA ≥17 nt. Supports RT-PCR, NGS, and microarray. Research use only.

United States

Texas

mbpinc