Nucleic Acid Extraction Kits

<h2>What are Spin-Column Bacterial and Fungal RNA Extraction Kits?</h2>
&nbsp;
Spin-column bacterial and fungal RNA extraction kits isolate total RNA from Gram-positive bacteria, Gram-negative bacteria, yeasts, filamentous fungi, algae, and protozoa using ultra-high-density BashingBead mechanical cell wall disruption in a chaotropic lysis buffer, followed by RNA binding to a Zymo-Spin silica-membrane column, on-column washing, and elution in 6-100 ul of RNase-free water - all using a standard microcentrifuge. The complete protocol takes under 15 minutes for a batch of 1-12 samples, requiring only a vortex with a microcentrifuge tube adapter, a microcentrifuge, and standard pipetting equipment. No specialized robotics, vacuum manifolds, or magnetic separation racks are needed. Choose a spin-column bacterial/fungal RNA kit for manual extraction of 1-24 samples per run, when existing automation infrastructure does not support magnetic bead formats, or when the fastest possible turnaround from culture to RT-qPCR data is required.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Pinpoint&trade; Slide RNA Isolation System for targeted extraction of total RNA directly from localized tissue sections on microscope slides
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Quick-RNA&trade; Fungal/Bacterial MicroPrep Kit for highly concentrated, low-volume recovery of transcripts utilizing micro-elution spin-column matrices&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
ZR Fungal/Bacterial RNA MiniPrep for precise cell-wall shattering of yeast and recalcitrant spores&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
YeaStar&trade; RNA Kit efficiently permeabilizes tough yeast cell walls.&nbsp;
</li>
</ul>
&nbsp;
<h2>How to Choose a Spin-Column Bacterial/Fungal RNA Kit</h2>
&nbsp;
Miniprep vs. Microprep Format
The Zymo Quick-RNA Fungal/Bacterial Miniprep processes up to 2x10^8 bacteria or 2x10^7 fungi per column, eluting in 25-100 ul - the standard choice for typical 1-5 ml bacterial cultures. The Microprep handles smaller inputs (up to 1x10^7 bacteria) and elutes in as little as 6 ul, concentrating RNA for sensitive applications such as single-cell-equivalent samples or very low-input transcriptomics.
Bead Size for Target Organism
Pre-mixed 0.1 mm + 0.5 mm BashingBead tubes in Zymo kits handle bacteria (0.1 mm) and fungi (0.5 mm) without selecting bead size. For pure Gram-negative bacterial cultures that do not require bead beating, direct lysis in buffer is available as a faster protocol option that skips the bead-beating step entirely.
On-Column DNase I Treatment
Include DNase I for all RT-qPCR and RNA-seq applications. Bacterial gDNA is abundant and co-purifies efficiently under silica-membrane binding conditions; without DNase treatment, gDNA contamination produces false amplification in RT-qPCR assays targeting genes with no introns. DNase I Reaction Mix is included in Zymo Quick-RNA Fungal/Bacterial kits.
Elution Volume
Elute in 25-50 ul for standard RT-qPCR (2-5 ul per reaction provides 5-25 RNA equivalents per assay). For RNA-seq library preparation requiring &gt;=200 ng input, use 50-100 ul elution volume to recover more total RNA mass; then measure concentration by Qubit and adjust for library prep.
Storage of Extracted RNA
Store spin-column bacterial RNA at -80C in single-use aliquots; mRNA from bacteria is AU-rich and degrades rapidly through multiple freeze-thaw cycles. Use within 6 months for transcriptomic applications; RT-qPCR targets with high expression levels remain quantifiable for 12+ months at -80C.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Target quality metrics for spin-column bacterial/fungal RNA: A260/A280 &gt;= 1.8, A260/A230 &gt;= 1.5, and RIN &gt;= 7 from log-phase cultures processed within 60 seconds of harvest. Zymo Research Quick-RNA Fungal/Bacterial kits use the same Zymo-Spin column technology found across the Quick-RNA product line and are validated for inhibitor-free RNA suitable for NGS, RT-PCR, microarray, and hybridization. The Quick-RNA Fungal/Bacterial Miniprep is one of the most widely used spin-column formats for bacterial and fungal RNA in academic molecular microbiology research, with validated protocols for S. cerevisiae, C. albicans, A. fumigatus, and standard Gram-positive and Gram-negative species.&nbsp;
<section class="mbp-contact-cta"></section>
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for kit selection guidance, bulk pricing, and Zymo Research protocol adaptation for non-standard organisms.

Spin-column

Bacterial/Fungal RNA/ Spin-column

MSDS

Protocol

Quick-RNA™ Fungal/Bacterial MicroPrep Kit (50 Preps.)

Quick-RNA™ Fungal/Bacterial MicroPrep Kit R2010 by Zymo Research — 50 preps, RNA ≥17 nt, 10 µg binding, ≥6 µL elution, ≤10 min. ZR BashingBead Lysis Tubes for mechanical disruption of fungal (Candida, Aspergillus, Saccharomyces) and bacterial cell walls. A260/A280 &gt;1.8. Order via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.

Applicable For

Elution Volume

Equipment

Processing Time

Processing Volume

Purity

Sample Source

Size Range

Type

Yield

Manufacturer

Shipping Condition

Storage Condition

<h2>What Are Spin-Column Total RNA Kits?</h2>
&nbsp;
Spin-column total RNA kits are the most widely used format for manual RNA purification in molecular biology laboratories. The technology relies on silica membrane chemistry: under chaotropic (guanidinium-based) lysis conditions, RNA adsorbs selectively to the silica membrane of the column; sequential ethanol-based wash steps remove proteins, salts, and gDNA fragments; and a low-ionic-strength buffer or RNase-free water then releases the purified RNA. Standard mini-format kits accept cell or tissue lysate from inputs of 1x10^4-1x10^7 cells or 5-100 mg tissue, bind up to 100 ug RNA, and complete the protocol in 15-30 minutes. The eluted RNA is suitable for RT-PCR, qRT-PCR, RNA sequencing, Northern blot, and hybridization without additional cleanup.&nbsp;Spin-column RNA kits are the default choice for 1-24 samples per run in any lab that does not require automated magnetic-bead processing.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Quick-RNA Microprep and Miniprep Kits for low-volume elution and highly concentrated recovery, providing ultra-clean and phenol-free transcript isolation
</li>
<li dir="ltr" aria-level="1">
Direct-zol&trade; RNA Kits to bypass tedious phase separation steps, allowing researchers to pump TRIzol-lysed samples directly into spin-columns&nbsp;
</li>
<li dir="ltr" aria-level="1">
Direct-zol&trade; DNA/RNA Parallel Modules are engineered for the non-destructive, simultaneous isolation of DNA and RNA fractions.
</li>
<li dir="ltr" aria-level="1">
ZymoBIOMICS&trade; RNA Mini Kits for uniform microbial profiling featuring host-zeroing lysis mechanics
</li>
<li dir="ltr" aria-level="1">
Quick-RNA&trade; Midiprep Assemblies to capture larger quantities of high-purity total transcripts efficiently, tailored for bulk processing&nbsp;
</li>
</ul>
&nbsp;
<h2>How to Choose Spin-Column Total RNA Kits</h2>
&nbsp;
Column Binding Capacity and Elution Volume
Mini-format columns bind up to 100 ug total RNA and elute in 10-100 uL. For samples with very low RNA content (needle biopsies, laser-capture microdissection sections, small cell numbers &lt;=10^4), choose micro-format columns with 8-12 uL minimum elution volume to maximize concentration. Eluting in &lt;30 uL with high RNA concentration increases sensitivity in downstream RT-qPCR.
Small RNA Retention
This is the most important differentiator between kit brands. Kits using Zymo-Spin column technology with proprietary binding buffers retain miRNA and small RNA species &gt;=17 nt in the total RNA fraction. Competing kits using standard high-ethanol wash chemistry typically lose RNA &lt;200 nt in the wash steps. If your workflow includes small RNA profiling, miRNA RT-qPCR, or small RNA-seq, verify that the kit retains &gt;=17 nt RNA before purchasing.
On-Column DNase Treatment
Included RNase-free DNase I that degrades gDNA on the column during the wash stage eliminates the need for post-elution DNase treatment. This matters for RT-qPCR with primers spanning a small intron, or for RNA-seq where even trace gDNA contamination generates false mapping reads. DNase-treated RNA typically passes a no-RT PCR control at Ct &gt;35.
Sample Type: Cells vs Tissue
Cell lysis buffer volumes and homogenization requirements differ by sample type. Cultured cells lyse directly in lysis buffer with vortexing; tissue samples require homogenization to disrupt cell structure before contact with the lysis buffer. For fibrous tissues (muscle, heart), use a bead-mill homogenizer or rotor-stator; for soft tissues (liver, brain), a disposable pestle or needle shearing is sufficient. Some kits include TissueLyser-compatible bead tubes or QIAshredder-equivalent homogenizer columns.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Spin-column total RNA purity targets: A260/A280 ratio 1.8-2.1; A260/A230 ratio &gt;1.8. RNA integrity number (RIN) from fresh cultured cells or snap-frozen tissue is routinely 8-10 with validated kits. Zymo Research Quick-RNA Mini and Miniprep kits are among the most cited spin-column RNA isolation kits in published RT-PCR and RNA-seq methods; ABM RNA extraction kits offer validated protocols for bacteria, yeast, and fungi not covered by many standard cell/tissue kits. All kits supplied by MBP are Research Use Only (RUO).
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

DataSheet

Quick-RNA™ MidiPrep (25 Preps.)

Quick-RNA MidiPrep Kit (R1056) by Zymo Research — up to 1 mg RNA yield per prep, ≥17 nt, A260/A280 and A260/A230 &gt;1.8, ≥200 µl elution. 25 Preps, room temperature storage. Optimized for cells and soft tissue.

<h2>What are Spin-Column Insect RNA Extraction Kits?</h2>
&nbsp;
Spin-column insect RNA extraction kits purify total RNA from whole arthropod specimens and insect tissue using ultra-high-density BashingBead mechanical homogenization in a chaotropic lysis buffer, followed by a two-column silica-membrane purification workflow in a standard microcentrifuge. The Zymo Quick-RNA Tissue/Insect Microprep Kit (R2030) uses a Zymo-Spin IIIC column to capture and discard genomic DNA in the flow-through, then a Zymo-Spin IC column to bind, wash, and elute total RNA, including small RNAs &gt;=17 nt. The full protocol completes in approximately 10 minutes for batches of 1-12 specimens. Choose the spin-column insect RNA format for manual extraction of single insects to small batches, for field work using DNA/RNA Shield stabilization, and for studies requiring co-isolation of miRNA and siRNA from the same arthropod specimen.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="bn9qob" data-start="0" data-end="116">Quick-RNA&trade; Tissue/Insect MicroPrep Kit for rapid isolation of ultra-pure total RNA from tissue and insect samples.</li>
<li data-section-id="1d6veh7" data-start="233" data-end="340">Ultra-clean spin-column technology designed to maximize RNA purity and recovery from small sample inputs.</li>
<li data-section-id="o7nhuv" data-start="341" data-end="463">RNA extraction solutions optimized for challenging biological specimens, including insect tissues and dissected samples.</li>
<li data-section-id="18gssqf" data-start="464" data-end="573">High-quality total RNA preparation suitable for RT-PCR, qPCR, RNA sequencing, and gene expression analysis.</li>
<li data-section-id="lpdewh" data-start="574" data-end="671" data-is-last-node="">Streamlined purification protocols that reduce processing time while maintaining RNA integrity</li>
</ul>
&nbsp;
<h2>How to Choose a Spin-Column Insect RNA Kit</h2>
&nbsp;
Microprep vs. Miniprep Format
The Microprep kit (Zymo R2030) processes specimens up to 10 mg and elutes in 6-25 ul, concentrating RNA for sensitive RT-qPCR from single small insects. The Miniprep format handles up to 30 mg of tissue - appropriate for dissected insect organs, larger arthropods, or small pools of 5-10 insects - and elutes in 25-100 ul for applications requiring higher total RNA mass.
Two-Column DNA Depletion vs. On-Column DNase
The two-column design (IIIC + IC column) in Zymo insect kits physically separates gDNA by binding capacity difference, eliminating a separate DNase incubation step. This is faster but less stringent than on-column DNase I treatment. For RNA-seq applications where any gDNA carryover introduces mapping artefacts, add an optional DNase I step; for RT-qPCR with intron-spanning primers, the two-column depletion alone is generally sufficient.
Bead Beating Instrument
A benchtop vortex (Vortex-Genie 2) with a horizontal microtube adapter provides validated lysis for the Zymo BashingBead Lysis Tubes at maximum speed for 5 minutes. A dedicated bead mill (e.g., Precellys, MP FastPrep) achieves more consistent disruption for hard-bodied arthropods such as hard ticks and beetles and is preferred when processing more than 24 specimens per session.
Specimen Stabilization Compatibility
Specimens in Zymo DNA/RNA Shield load directly into lysis tubes without additional washes or centrifugation steps. If specimens were field-collected in dry ice or liquid nitrogen, thaw at room temperature in lysis buffer and add to BashingBead tubes immediately - do not allow thawed specimens to sit at room temperature without lysis buffer, as RNase activity resumes rapidly on thawing.
Vector Surveillance Pooling
For arbovirus surveillance, pool up to 50 mosquitoes in the same lysis tube, cap tightly, and bead-beat at maximum speed for 10 minutes. Transfer 200-400 ul of clarified lysate (post-centrifugation) per column; multiple column reloads can process the remaining volume. Validate pool sensitivity with a spiked positive control before deploying in routine surveillance.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Performance benchmarks for spin-column insect RNA: A260/A280 &gt;= 1.8, A260/A230 &gt;= 1.8, and RNA yield up to 10 ug from a single Aedes aegypti adult female. The Zymo Quick-RNA Tissue/Insect Microprep Kit is validated for RT-PCR, hybridization, microarray, and Next-Gen sequencing applications, and its two-column architecture is designed to deliver RNA free of PCR inhibitors - a critical requirement for arbovirus RT-qPCR assays where false-negative results from inhibitor carryover have direct public health implications. This kit is the primary insect RNA extraction format used in Drosophila immunity and mosquito vector competence research, with published protocols referencing BashingBead lysis in studies from institutions including Howard Hughes Medical Institute and Vanderbilt University.
<section class="mbp-contact-cta">
<h2>&nbsp;</h2>
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Spin-Column

Quick-RNA™ Tissue/Insect MicroPrep Kit (50 Preps.)

Quick-RNA™ Tissue/Insect MicroPrep Kit R2030 by Zymo Research is a 50-prep kit for total RNA isolation (≥17 nt, including miRNAs) from insects, arthropods (mosquitoes, ticks, Drosophila), and tough-to-lyse tissue in 1–20 mg amounts. Includes BashingBead lysis tubes, Zymo-Spin IC and IIICG columns, minimum elution 6 µl, binding capacity 10 µg, purity A260/A280 >1.8. All components stored at room temperature.

<h2>What Are Cell-Free DNA Extraction Kits?</h2>
&nbsp;
A cell-free DNA (cfDNA) extraction kit purifies circulating cfDNA fragments from plasma, serum, or urine using silica spin-column or magnetic bead-based technologies. These kits are optimized to recover the short DNA fragments commonly found in liquid biopsy samples for downstream applications such as PCR, qPCR, next-generation sequencing (NGS), and methylation analysis. Choose a cfDNA-specific kit over a general genomic DNA kit whenever short circulating DNA fragments are the primary target.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>MagBio cfKapture ccfDNA Extraction Kit: for cell-free DNA isolation using advanced magnetic bead-based technology.</li>
<li>MAGicBead&trade; cfDNA Isolation Kit: for automated, high-throughput liquid biopsy workflows using high-binding paramagnetic particles.</li>
<li>Quick-cfDNA&trade; Serum &amp; Plasma Kit: for streamlined manual benchtop purification using rapid spin-column technology.</li>
</ul>
&nbsp;
<h2>How to Choose a Cell-Free DNA Extraction Kit</h2>
&nbsp;
Input Volume Range
Choose a kit compatible with your typical plasma, serum, or urine input volume. Larger sample volumes generally increase total cfDNA recovery but may require additional reagents and longer processing times.
Column-Based vs. Magnetic Bead-Based Chemistry
Silica spin-column kits are widely used for manual purification workflows, while magnetic bead-based kits simplify automation and high-throughput processing on compatible liquid handling platforms.
Elution Volume for Downstream Assays
Select an elution volume that matches the input requirements of your downstream applications, such as PCR, qPCR, or NGS library preparation, to minimize additional concentration or dilution steps.
Combined DNA/RNA Recovery
If your workflow requires analysis of both circulating DNA and RNA from the same specimen, consider kits designed to recover multiple nucleic acid types from a single sample.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Key considerations when selecting a cell-free DNA extraction kit include sample input volume, purification chemistry, compatibility with manual or automated workflows, and elution volume for downstream molecular assays. Magnetic bead-based systems are commonly selected for scalable, automated liquid biopsy workflows, while spin-column kits provide reliable manual purification for routine laboratory use. The optimal kit depends on your sample type, throughput requirements, and downstream analytical methods.
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Cell-Free DNA

D4076-Data Sheet

D4076 - MSDS

D4076-1-32 - MSDS

D4076-2-170 - MSDS

D4076-3-10 - MSDS

D4076-4-12 - MSDS

D4076-A - MSDS

D4076-Protocol

Quick-cfDNA™ Serum & Plasma Kit

Quick-cfDNA™ Serum &amp; Plasma Kit (D4076) by Zymo Research — 50 preps, purifies cfDNA ≥100 bp from up to 10 ml serum/plasma. Also compatible with amniotic fluid, CSF, and saliva. Elution ≥35 µl. Compatible with Streck and PAXgene tubes.

DNA Purity

DNA Recovery

DNA Storage

Processing volume

Recovery Volume

Required Equipment

Sample Sources

Quick-cfDNA™ Serum & Plasma Kit (50 Preps.)

S&P 5X Digestion Buffer (32 ml)

S&P DNA Binding Buffer (170 ml)

S&P DNA Prep Buffer (10 ml)

S&P DNA Wash Buffer (Concentrate) (12 ml)

Quick-cfDNA™ Serum & Plasma Buffer Set

<h2>What are Cell-Free RNA Extraction Kits?</h2>
&nbsp;
Cell-free RNA (cfRNA) extraction kits isolate circulating RNA molecules - microRNAs, long non-coding RNAs, exosomal RNA, and mRNA fragments - from blood plasma, serum, urine, cerebrospinal fluid, and saliva, where endogenous RNA concentrations range from 1-100 ng/ml. Unlike standard total RNA kits designed for cell lysates containing micrograms of intracellular RNA per sample, cell-free RNA kits are built for the low-abundance, fragmented, and often vesicle-packaged RNA species present in acellular body fluids. They incorporate carrier RNA to prevent adsorption loss, handle input volumes of 200 ul to 5 mL, and use binding chemistries optimized for small RNAs &lt;200 nt. Use a cell-free RNA kit - not a standard total RNA or viral RNA kit - whenever your input is plasma, serum, urine, or another cell-depleted biofluid and your target is circulating miRNA, cfRNA, or exosomal RNA.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="1fsegxt" data-start="25" data-end="105">Rapid purification of circulating cell-free RNA (cfRNA) from plasma and serum.</li>
<li data-section-id="dahqiz" data-start="106" data-end="181">Efficient recovery of low-abundance cell-free RNA from biological fluids.</li>
<li data-section-id="85g4hb" data-start="182" data-end="245">Zymo-Spin&trade; spin-column technology for reliable RNA isolation.</li>
<li data-section-id="exy4np" data-start="246" data-end="296">Streamlined workflow for fast sample processing.</li>
<li data-section-id="mtq4uw" data-start="297" data-end="369" data-is-last-node="">Purified cfRNA suitable for downstream molecular biology applications.</li>
</ul>
&nbsp;
<h2>How to Choose a Cell-Free RNA Extraction Kit</h2>
&nbsp;
Input Volume and Throughput
Standard cell-free RNA kits accept 200 &mu;L-1 mL of plasma or serum per extraction; this input volume is appropriate for RT-qPCR quantification of abundant miRNA species and exosomal RNA panels. For discovery-phase cfRNA-seq, where rare transcripts are targets, use high-volume extraction formats to increase RNA mass from low-abundance circulating species.
Small RNA Enrichment
Circulating miRNAs are the most studied cfRNA analytes; kits that enrich for RNA &lt;200 nt deliver higher sensitivity for miRNA panels than kits recovering large and small RNAs together.&nbsp;
Carrier RNA and Spike-In Controls
Every cell-free RNA extraction should include both a carrier RNA (added to the lysis buffer to improve recovery) and an exogenous spike-in control RNA - typically Caenorhabditis elegans miR-39 (cel-miR-39) at 1.6 &times; 10^8 copies per extraction - added at the lysis step. Spike-ins normalize inter-sample variation in extraction efficiency and enable detection of failed extractions. Confirm whether carrier RNA is supplied in the kit or purchased separately.
Magnetic Bead vs. Spin-Column Format
Spin-column cfRNA kits (Zymo Quick-RNA Serum &amp; Plasma) are the standard for 1-24 samples per session and require only a microcentrifuge. Magnetic bead formats are preferred for higher-volume inputs and automation, and demonstrate superior recovery of fragmented low-abundance RNA from dilute matrices in published comparative studies.
Phenol-Free vs. Phenol-Containing Lysis
Most current cell-free RNA kits use guanidinium-based, phenol-free lysis buffers, avoiding the hazardous handling requirements of phenol-containing reagents. Phenol-chloroform-based extraction of cfRNA is used in research settings for the highest yield but introduces extraction bias for certain miRNA species and requires additional purification steps; column-based phenol-free kits are preferred for clinical and high-throughput biomarker applications.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Performance benchmarks for cell-free RNA extraction: recovery of cel-miR-39 spike-in within 2 Ct of the expected value, A260/A280 &gt;= 1.7 (lower values are acceptable for extremely dilute cfRNA samples), and consistent inter-sample Ct variation &lt;1.5 Ct for reference miRNAs across a batch. Circulating miRNA biomarkers -- including miR-21, miR-155, miR-210, miR-126, and miR-200 family members - are under clinical evaluation as non-invasive cancer, cardiovascular, and neurological disease biomarkers, with studies published by groups at institutions including MD Anderson Cancer Center and Vanderbilt University. Pre-analytical variables -- plasma preparation method, freeze-thaw cycles, hemolysis - are major sources of inter-study variability in cfRNA data; standardized two-spin plasma preparation protocols are recommended before extraction.
&nbsp;
For ordering assistance, bulk pricing, or protocol guidance on selecting the right kit for your sample type, <a href="https://mbpinc.net/contact-us">contact the MBP team</a>.&nbsp;

Cell-free RNA

White Paper

Quick-cfRNA™ Serum and Plasma (50 Preps.)

Quick-cfRNA™ Serum and Plasma Kit (R1059) by Zymo Research isolates cell-free RNA (≥17 nt, including microRNA) from up to 3 mL of plasma, serum, CSF, amniotic fluid, or urine in a spin-column workflow with 6–15 µl concentrated elution. No phenol-chloroform required. 50-prep format for liquid biopsy and cfRNA biomarker research.

Sample Type

Size

<h2>What are microbial and environmental DNA isolation kits?</h2>
&nbsp;
Microbial and environmental DNA isolation kits purify DNA from soil, sediment, freshwater, marine water, feces, and other complex environmental matrices using lysis and purification chemistry designed to remove humic acids, metal compounds, and other inhibitors that interfere with PCR and sequencing, supporting 16S/18S rRNA amplicon profiling and shotgun metagenomic sequencing. Related entities include "kitome" and "splashome" contamination controls, alpha diversity and microbial community composition analysis, and compatibility with both short-read (Illumina) and long-read (Oxford Nanopore) sequencing platforms. Choose a kit validated for your specific sample matrix (soil, sediment, water, or feces), since benchmarking studies consistently show kit performance varies by matrix rather than one kit being universally best.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Spin-column fungal and bacterial kits for rapid DNA purification using rapid silica-membrane technology&nbsp;</li>
<li dir="ltr" role="presentation">Parallel extraction of soil, fecal, and environmental samples using high-throughput 96-well microplates&nbsp;</li>
<li dir="ltr" role="presentation">Automated&nbsp; purification of complex microbial and environmental specimens using Magnetic bead isolation systems</li>
<li dir="ltr" role="presentation">Scalable microprep and midiprep columns tailored for concentrated low-volume elution demands and diverse sample inputs</li>
</ul>
&nbsp;
<h2>How to choose a microbial and environmental DNA isolation kit</h2>
&nbsp;
Matrix-specific kit performance
Benchmarking studies comparing multiple commercial kits across sediment, water, soil, oyster gut microbiome, and mammalian feces have found that the best-performing kit varies by matrix - kits ranked highly for sediment did not necessarily perform best for water or host-associated samples. Rather than assuming a single "universal" kit, match your kit choice to published benchmarking results for your primary sample type where available.
Inhibitor removal for downstream PCR and sequencing
Environmental samples, especially sediments and soils, contain organic and metal compounds that inhibit PCR amplification if not removed during extraction; kits designed for these matrices integrate inhibitor-removal steps as part of the standard protocol. If your samples have historically produced low PCR amplification efficiency with general-purpose kits, an environmental-matrix-specific kit with built-in inhibitor removal is the first thing to evaluate.
Short-read vs. long-read sequencing compatibility
For long-read metagenomic sequencing, DNA fragment length and integrity from the extraction kit directly affect achievable read lengths, though longer extracted fragments don't always translate consistently into longer sequencing reads - kit choice affects both fragment length and downstream classification rate and reproducibility. If long-read metagenomics is your goal, prioritize kits with published long-read benchmarking data over kits validated only for short-read amplicon work.
Contamination control (kitome/splashome)
Kit-derived contamination ("kitome") and cross-sample contamination during processing ("splashome") can affect microbial community composition results, particularly for low-biomass samples; benchmarking studies evaluate kits specifically for these contamination sources alongside yield and diversity metrics. For low-biomass environmental samples, prioritize kits with documented low kitome contamination in published comparisons.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for microbial and environmental DNA isolation are matrix-specific validation (soil vs. sediment vs. water vs. feces), built-in inhibitor removal for PCR-ready output, and fragment length/integrity for long-read sequencing compatibility where relevant. Since benchmarking studies repeatedly show no single kit performs best across all matrices, identifying your primary sample type before selecting a kit is the most impactful decision. Labs running metagenomic surveys across multiple matrices (e.g., soil and water from the same field site) often need separate matrix-optimized kits rather than a single universal kit. The parent <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits hub</a>&nbsp;links to related sample-type categories, including&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/insect">insect genomic DNA kits</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/plant">plant genomic DNA kits</a>&nbsp;for organism-associated environmental work, and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/high-molecular-weight-dna">high molecular weight DNA kits</a>&nbsp;for long-read metagenomics requiring larger fragment sizes. For cell-free DNA in environmental water samples (eDNA detection), see&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/cell-free-dna">cell-free DNA kits</a>.&nbsp;
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Microbial & Environmental DNA Isolation

YeaStar™ Genomic DNA Kit

D2002 by Zymo Research is the YeaStar™ Genomic DNA Kit, a 40-prep spin-column kit designed for simple, total genomic DNA purification from a broad spectrum of Zymolyase-susceptible fungal species, including Saccharomyces cerevisiae, Candida, Pichia, and more than a dozen additional genera. Each prep yields up to 25 µg of high-molecular-weight fungal gDNA (35–60 kb) at A260/A280 ≥ 1.8, processed in approximately 1.5 hours from culture to elution.

YeaStar™ Genomic DNA Kit (40 Preps.)

YD Digestion Buffer (4.8 ml)

YD Lysis Buffer (4.8 ml)

YeaStar™ RNA Kit (40 Preps.)

YeaStar™ RNA Kit R1002 by Zymo Research — 40-prep yeast RNA isolation kit, ≤25 µg yield, A260/A280 ≥1.8, 30-min protocol, compatible with S. cerevisiae, Candida, Aspergillus, Pichia, and Schizosaccharomyces. Ships from MBP INC to USA, Canada, and international labs.

<h2>What are plasmid miniprep kits?</h2>
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A plasmid miniprep kit uses SDS/alkaline lysis to break open pelleted E. coli cells from a small culture volume (1-10 ml), followed by binding to a silica-membrane spin column, washing, and elution, separating plasmid DNA from genomic DNA, RNA, and protein in roughly 10-15 minutes per prep. Related entities include Buffer P1/P2/P3-style alkaline lysis reagents, color-coded lysis dyes for visual confirmation of complete lysis and neutralization, RNase A for RNA removal, and downstream applications including transformation, restriction digestion, PCR, and sequencing. Choose a miniprep kit for routine, low-volume plasmid prep (cloning screens, sequencing checks, restriction digests) where yields up to roughly 20-100 ug per column are sufficient.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Pellet-free and classic plasmid miniprep kits for high-yield bacterial isolation using rapid spin-column workflows</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">High-throughput 96-well plasmid plates optimized for vacuum manifold processing and parallel automated screening&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Specialized yeast plasmid miniprep systems for fragile fungal hosts using cell-wall digesting chemistries</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">BAC/PAC and large construct isolation units for safer purification of high-molecular-weight vectors</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Endotoxin-free plasmid extraction reagents for sensitive mammalian cell lines, ensuring transfection-grade DNA purity&nbsp;</li>
</ul>
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<h2>How to choose a plasmid miniprep kit</h2>
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Standard vs. high-yield chemistry
Standard silica spin-column minipreps typically recover up to about 20 ug per column, while high-yield formulations using modified alkaline lysis and novel binding chemistry can reach up to 100 ug at concentrations up to 3 ug/ul from the same small culture volume. If your downstream application (e.g., sequencing multiple samples from one prep, or transfection requiring more DNA per prep) benefits from a higher concentration, a high-yield kit reduces the need to scale up to midiprep.
Plasmid copy number and culture volume planning
High-copy plasmids (pUC-based vectors) can saturate a miniprep column from as little as 3 ml of culture, yielding around 20 ug, while low-copy plasmids (pBR322, pET, cosmids) may yield under 2 ug from the same volume and benefit from doubling culture volume to 5-10 ml. Identify your vector's origin of replication before choosing a standard culture volume, since low-copy vectors at standard volumes can produce yields too low for some downstream steps.
Color-coded lysis buffers for visual QC
Some miniprep kits include color-coded lysis and neutralization buffers that allow visual confirmation of complete cell lysis (color change) and proper neutralization before loading the column, helping catch incomplete-lysis failures before they waste a column. This visual QC step is particularly useful for new users or when troubleshooting inconsistent yields across a batch of preps.
Endotoxin-free and large-construct options
For transfection-sensitive downstream applications, endotoxin-free miniprep kits add a dedicated wash step without significantly extending the protocol time; for large plasmids, cosmids, or BAC/YAC/PAC constructs, some miniprep kits are validated up to approximately 200 kb, while standard kits are optimized for constructs up to roughly 25 kb. Confirm both endotoxin status and size compatibility match your specific construct and downstream use before relying on a standard miniprep.
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<h2>Specifications context</h2>
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The key specs for plasmid miniprep selection are expected yield relative to your plasmid's copy number and culture volume, whether color-coded buffers aid visual QC, and whether endotoxin-free or large-construct compatibility is needed for your downstream application. A standard miniprep yielding up to roughly 20-25 ug in 10-15 minutes from 1-10 ml of culture covers most routine cloning workflows, while high-yield kits up to 100 ug reduce the need to scale up for moderately DNA-hungry applications. Labs running both daily cloning screens and occasional larger-scale prep work typically keep <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/miniprep-kits">miniprep kits</a>&nbsp;on hand alongside&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/midiprep-kits">midiprep kits</a>&nbsp;for when miniprep yields aren't sufficient. The parent&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits">plasmid DNA extraction kits category</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/maxiprep-kits">maxiprep kits</a>&nbsp;cover larger-scale options, while the broader&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits">nucleic acid extraction kits hub</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits category</a> link to chromosomal DNA workflows.&nbsp;
<section class="mbp-contact-cta">
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MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Miniprep Kits

ZR BAC DNA Miniprep Kit

D4048 by Zymo Research — ZR BAC DNA Miniprep Kit (25 preps) for rapid isolation of BAC, PAC, fosmid, and cosmid DNA up to ~200 kb from E. coli. 15-minute protocol; elution ≥ 10 µl; yield up to 10 µg per prep; purity A260/A280 ≥ 1.8, A260/A230 ≥ 2.0. Includes colored buffer system for visual lysis confirmation.

ZR BAC DNA Miniprep Kit (25 Preps.)

ZR BAC DNA Miniprep Kit (100 Preps.)

ZR Fungal/Bacterial RNA MiniPrep (50 Preps.)

ZR Fungal/Bacterial RNA MiniPrep (R2014) by Zymo Research isolates up to 50 µg of total RNA (≥17 nt) from gram-positive bacteria, fungi, yeast, spores, and pollen in 10 minutes or less using a bead-beating lysis step followed by spin-column purification. Purity exceeds A260/A280 of 1.8, and purified RNA is ready for RT-qPCR, RNA-seq, and microarray applications. 50-prep format.

Product Literature

ZR Plasmid Miniprep - Classic

ZR Plasmid Miniprep Classic D4015 by Zymo Research isolates up to 25 µg of plasmid DNA from up to 5 mL of overnight E. coli culture using a traditional three-buffer alkaline lysis protocol in approximately 15 minutes, with A260/280 ≥1.8 purity and ≤50 EU/µg endotoxin. 100 preps per kit.

Endotoxin Levels

ZR Plasmid Miniprep - Classic (100 Preps.)

ZR Plasmid Miniprep - Classic (400 Preps.)

ZR Plasmid Miniprep-Classic (800 Preps.)

<h2>What are Spin-Column Environmental RNA Extraction Kits?</h2>
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Spin-column environmental RNA extraction kits purify total RNA from soil, fecal material, water concentrates, biofilm, and plant tissue using bead-beating mechanical disruption in a chaotropic lysis buffer, followed by silica-membrane column purification incorporating inhibitor-removal wash steps to eliminate humic acids, polyphenols, and other PCR inhibitors co-extracted from environmental matrices. Kits process 0.1-0.5 g of sample per extraction and elute RNA in 30-100 ul of RNase-free water in approximately 2 hours, producing RNA suitable for RT-qPCR, microarray, and RNA-seq metatranscriptomic workflows. Choose the spin-column format for environmental RNA when processing 1-24 samples per day, when the sample type is soil, feces, or biofilm, or when standard bacterial/fungal RNA kits produce inhibited RT-qPCR results from environmental matrices.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
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<ul>
<li data-section-id="1b52a6l" data-start="0" data-end="128">ZR Soil/Fecal RNA MicroPrep Kit for efficient recovery of inhibitor-free cellular RNA from challenging soil and fecal samples.</li>
<li data-section-id="120xy2g" data-start="129" data-end="275">Zymo Environ Water RNA Kit for purification of microbial RNA from turbid water samples used in environmental monitoring and microbiome research.</li>
<li data-section-id="tojsbj" data-start="276" data-end="400">ZR Urine RNA Isolation Kit&trade; for rapid extraction of high-quality total RNA from urine using silica spin-column technology.</li>
<li data-section-id="2q9aey" data-start="401" data-end="525">RNA purification solutions tailored to environmental and liquid biopsy sample types with distinct processing requirements.</li>
<li data-section-id="7kn372" data-start="526" data-end="647">Specialized extraction workflows designed to remove contaminants that can interfere with downstream molecular analyses.</li>
<li data-section-id="1gyfm8m" data-start="648" data-end="753" data-is-last-node="">High-quality RNA isolation products suitable for RT-PCR, qPCR, sequencing, and gene expression studies.</li>
</ul>
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<h2>How to Choose a Spin-Column Environmental RNA Kit</h2>
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Inhibitor Load of Your Sample Type
High-humic soils (peat, forest soils, dark topsoil) require kits with dedicated IRT or inhibitor-precipitating wash steps, such as the QIAGEN RNeasy PowerSoil Total RNA Kit. For fecal and biofilm samples with lower humic content, the faster Zymo Quick-RNA Fecal/Soil Microbe Microprep Kit is sufficient. An A260/A230 ratio &lt; 1.5 in the eluate is a direct signal that the kit's inhibitor removal is insufficient for your matrix.
Protocol Time and Throughput
The Zymo Fecal/Soil Microprep Kit extracts in approximately 2 hours using mini-spin columns. The QIAGEN PowerSoil Total RNA Kit takes 5-7 hours but delivers higher RIN values for long-read sequencing applications. For batches of more than 12 samples per day, PowerBead Pro or Zymo's mini-column format with a vortex adapter is faster per sample than protocols requiring individual mechanical homogenization steps.
Downstream Application: Short-read vs. Long-read Sequencing
For short-read RNA-seq (Illumina), RIN &gt;= 5 is generally sufficient because library preparation using random hexamer priming tolerates fragmented RNA. For Oxford Nanopore direct RNA sequencing, which reads intact RNA molecules, RIN &gt;= 7 is required; only the QIAGEN PowerSoil kit consistently achieves this benchmark across diverse soil types in published comparisons.
Bead Type and Cell Disruption
Soil and fecal kits use ceramic or glass beads at 0.1-0.5 mm diameter. Confirm the kit includes both 0.1 mm and 0.5 mm beads to ensure unbiased lysis of Gram-negative bacteria (disrupted efficiently by 0.1 mm beads) and Gram-positive bacteria and fungi (requiring 0.5 mm beads) in the same sample. Single-bead-size kits can produce a bias toward Gram-negative recovery in mixed environmental communities.
Compatibility with rRNA Depletion
Residual inhibitors from soil RNA extract can impair enzymatic rRNA depletion reactions. If initial rRNA depletion efficiency is low (&lt;90%), re-clean the extract with a Zymo RNA Clean &amp; Concentrator-5 kit before repeating the depletion reaction. Check kit-specific guidance from your rRNA depletion kit supplier.
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<h2>Specifications Context</h2>
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Key benchmarks for spin-column environmental RNA: A260/A280 &gt;= 1.8, A260/A230 &gt;= 1.5 (target &gt;= 1.8 for long-read sequencing), and concentration &gt;= 10 ng/ul in the eluate for RT-qPCR assays. A 2024 peer-reviewed comparison in&nbsp;Microbiology Society Journals specifically evaluated five commercial soil RNA extraction kits for Oxford Nanopore metatranscriptomics and identified the QIAGEN PowerSoil kit as the highest performer across RNA yield, integrity, and purity metrics. Soil metatranscriptomics is increasingly used in climate change research - linking active microbial community function to carbon and nitrogen cycling - and environmental RNA extraction quality is cited in published methods as the rate-limiting step for generating sequencing-ready RNA from soil.
<section class="mbp-contact-cta"></section>
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP Inc. today and get high-yield spin-column systems for your lab.

MSDS - R2040-1

ZR Soil/Fecal RNA MicroPrep (50 Preps.)

R2040 by Zymo Research is the ZR Soil/Fecal RNA MicroPrep Kit, a 50-prep RNA extraction system for up to 250 mg of soil, sludge, sediment, or fecal material. The kit recovers total RNA &ge;17 nucleotides — including small RNAs and miRNAs — at up to 10 &micro;g per column, with a standard 15 &micro;l elution volume (minimum 6 &micro;l for concentrated samples) and A260/A280 &gt;1.8 purity. All components are stored at room temperature.
The kit includes ZR BashingBead Lysis Tubes (0.1 &amp; 0.5 mm bead mix) and an HRC filtration step that removes PCR inhibitors including humic acids, polyphenols, and tannins from complex environmental matrices. Suitable for next-generation sequencing, RT-PCR, qPCR, and microarray.
MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, Eurofins, and many other institutes across the USA. Order via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or contact <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.

S/F RNA Lysis Buffer (50 ml)

MSDS - R1038/R1039

MSDS - R1038-1

MSDS - R1038-2

ZR Urine RNA Isolation Kit™

R1038 by Zymo Research is the ZR Urine RNA Isolation Kit™ (20 preps) for total RNA isolation — including small RNAs ≥17 nt — from urine and aqueous samples containing cells, sediment, and microvesicles. Zymo-Spin IC columns; elution ≥6 µl; purity A260/A280 &gt;1.8. Suitable for urinary biomarker and urological disease RNA research.