Nucleic Acid Extraction Kits

<h2>What are plasmid midiprep kits?</h2>
&nbsp;
A plasmid midiprep kit scales SDS/alkaline lysis and silica-membrane column binding (or anion-exchange resin with gravity flow) to 150-300 ml of overnight E. coli culture, recovering 0.5-2 mg of plasmid DNA with lysate clearing accomplished by high-speed centrifugation followed by a clearing column or filtration step, completable in as little as 30 minutes for some spin/vacuum formats. Related entities include high-speed centrifugation for lysate clearing, NucleoSnap-type midi formats, endotoxin removal washes, and downstream applications including moderate-scale transfection and multi-reaction restriction/sequencing work. Choose midiprep when miniprep yields (up to ~25-100 ug) aren't sufficient, but full maxiprep-scale culture volume (100-500 ml) and processing time aren't necessary.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Rapid 25-minute midiprep kits to bypass lengthy gravity-flow precipitation steps utilizing accelerated column dynamics</li>
<li dir="ltr" role="presentation">Vacuum-assisted syringe-filter plasmid midi modules engineered for rapid and debris-free clearing of lysates without manual pelleting</li>
<li dir="ltr" role="presentation">Endotoxin-free intermediate purification systems for sensitive mammalian cell screening by delivering transfection-grade constructs</li>
<li dir="ltr" role="presentation">High-purity silica spin-column configurations optimized for elution of high-copy vectors&nbsp;</li>
<li dir="ltr" role="presentation">Advanced anion-exchange gravity-flow assemblies provide template purity for downstream sequencing.</li>
</ul>
&nbsp;
<h2>How to choose a plasmid midiprep kit</h2>
&nbsp;
When midiprep fits between mini and maxi
Midiprep format is the right choice when your downstream application needs more DNA than a miniprep provides (roughly 20-100 ug depending on chemistry) but doesn't require the largest maxiprep-scale yields (up to 1 mg or more); 0.5-2 mg from 150-300 ml culture covers many moderate-scale transfection experiments and multi-replicate downstream reactions. If you find yourself running multiple minipreps to pool DNA for a single transfection, a single midiprep is often more efficient.
Lysate clearing method
Midiprep kits clear lysate using high-speed centrifugation followed by a clearing column (spin or vacuum format) or filtration through folded filters in anion-exchange systems; spin/vacuum clearing formats can complete the full protocol in as little as 30 minutes, while gravity-flow anion-exchange systems may take longer but avoid centrifugation steps for the binding/wash/elute portion. Match the clearing method to your lab's available centrifuge capacity (rotor size for 150-300 ml tubes) and time constraints.
Endotoxin-free midiprep options
For transfection of sensitive cell types, endotoxin-free midiprep kits using anion-exchange chemistry can achieve endotoxin removal in a unique washing step without additional incubations, suitable for transfection of highly sensitive cells, vaccination research, or gene therapy applications. If your midi-scale prep feeds directly into sensitive-cell transfection, confirm the kit's endotoxin specification (e.g., below 0.1 EU/ug) against your cell type's documented sensitivity.
Plasmid copy number and culture volume within the midi range
As with miniprep, plasmid copy number affects yield within the midiprep culture volume range - low-copy plasmids may need culture volumes toward the higher end (300 ml) to reach typical midi yields, while high-copy plasmids may reach 1-2 mg even at 150 ml. Plan culture volume within the midi range based on your specific vector rather than defaulting to the kit's maximum stated volume.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for midiprep selection are whether 0.5-2 mg from 150-300 ml culture meets your downstream DNA quantity needs without over- or under-scaling, lysate clearing method (centrifugation-based spin/vacuum vs. gravity-flow anion-exchange), and endotoxin status for transfection-sensitive applications. A midiprep completing in as little as 30 minutes via spin/vacuum clearing offers a time advantage over gravity-flow systems while still avoiding the larger culture volumes of maxiprep. Labs whose miniprep yields are consistently insufficient for their transfection protocols typically move to <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/midiprep-kits">midiprep kits</a>&nbsp;before considering&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/maxiprep-kits">maxiprep kits</a>, which require substantially more culture volume and processing time. The parent&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits">plasmid DNA extraction kits category</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/miniprep-kits">miniprep kits</a>&nbsp;cover smaller-scale options, while the broader&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits">nucleic acid extraction kits hub</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits category</a> link to chromosomal DNA workflows.
&nbsp;
<section class="mbp-contact-cta">
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Midiprep Kits

DataSheet

D4201 - MSDS

D4201 (D4200/D4201)

ZymoPURE™ II Plasmid Midiprep Kit

D4201 by Zymo Research: ZymoPURE™ II Plasmid Midiprep Kit, 50 preps. Processes ≤50 mL E. coli culture; yields 200–400 µg; ≤18 min protocol. Endotoxin ≤0.025 EU/µg (EndoZero II) or ≤1 EU/µg standard. Up to 200 kb; A260/A280 ≥1.8. Made in USA.

Applicable For

Binding Capacity

Culture Input

Elution Volume

Endotoxin Levels

Equipment

Format

Processing Time

Purity

Size Range

Yield

Manufacturer

Shipping Condition

Storage Condition

ZymoPURE™ II Plasmid Midiprep Kit (50 Preps.)

ZymoPURE™ II Plasmid Midiprep Kit (25 Preps.)

<h2>What are plasmid miniprep kits?</h2>
&nbsp;
A plasmid miniprep kit uses SDS/alkaline lysis to break open pelleted E. coli cells from a small culture volume (1-10 ml), followed by binding to a silica-membrane spin column, washing, and elution, separating plasmid DNA from genomic DNA, RNA, and protein in roughly 10-15 minutes per prep. Related entities include Buffer P1/P2/P3-style alkaline lysis reagents, color-coded lysis dyes for visual confirmation of complete lysis and neutralization, RNase A for RNA removal, and downstream applications including transformation, restriction digestion, PCR, and sequencing. Choose a miniprep kit for routine, low-volume plasmid prep (cloning screens, sequencing checks, restriction digests) where yields up to roughly 20-100 ug per column are sufficient.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Pellet-free and classic plasmid miniprep kits for high-yield bacterial isolation using rapid spin-column workflows</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">High-throughput 96-well plasmid plates optimized for vacuum manifold processing and parallel automated screening&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Specialized yeast plasmid miniprep systems for fragile fungal hosts using cell-wall digesting chemistries</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">BAC/PAC and large construct isolation units for safer purification of high-molecular-weight vectors</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Endotoxin-free plasmid extraction reagents for sensitive mammalian cell lines, ensuring transfection-grade DNA purity&nbsp;</li>
</ul>
&nbsp;
<h2>How to choose a plasmid miniprep kit</h2>
&nbsp;
Standard vs. high-yield chemistry
Standard silica spin-column minipreps typically recover up to about 20 ug per column, while high-yield formulations using modified alkaline lysis and novel binding chemistry can reach up to 100 ug at concentrations up to 3 ug/ul from the same small culture volume. If your downstream application (e.g., sequencing multiple samples from one prep, or transfection requiring more DNA per prep) benefits from a higher concentration, a high-yield kit reduces the need to scale up to midiprep.
Plasmid copy number and culture volume planning
High-copy plasmids (pUC-based vectors) can saturate a miniprep column from as little as 3 ml of culture, yielding around 20 ug, while low-copy plasmids (pBR322, pET, cosmids) may yield under 2 ug from the same volume and benefit from doubling culture volume to 5-10 ml. Identify your vector's origin of replication before choosing a standard culture volume, since low-copy vectors at standard volumes can produce yields too low for some downstream steps.
Color-coded lysis buffers for visual QC
Some miniprep kits include color-coded lysis and neutralization buffers that allow visual confirmation of complete cell lysis (color change) and proper neutralization before loading the column, helping catch incomplete-lysis failures before they waste a column. This visual QC step is particularly useful for new users or when troubleshooting inconsistent yields across a batch of preps.
Endotoxin-free and large-construct options
For transfection-sensitive downstream applications, endotoxin-free miniprep kits add a dedicated wash step without significantly extending the protocol time; for large plasmids, cosmids, or BAC/YAC/PAC constructs, some miniprep kits are validated up to approximately 200 kb, while standard kits are optimized for constructs up to roughly 25 kb. Confirm both endotoxin status and size compatibility match your specific construct and downstream use before relying on a standard miniprep.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for plasmid miniprep selection are expected yield relative to your plasmid's copy number and culture volume, whether color-coded buffers aid visual QC, and whether endotoxin-free or large-construct compatibility is needed for your downstream application. A standard miniprep yielding up to roughly 20-25 ug in 10-15 minutes from 1-10 ml of culture covers most routine cloning workflows, while high-yield kits up to 100 ug reduce the need to scale up for moderately DNA-hungry applications. Labs running both daily cloning screens and occasional larger-scale prep work typically keep <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/miniprep-kits">miniprep kits</a>&nbsp;on hand alongside&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/midiprep-kits">midiprep kits</a>&nbsp;for when miniprep yields aren't sufficient. The parent&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits">plasmid DNA extraction kits category</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/maxiprep-kits">maxiprep kits</a>&nbsp;cover larger-scale options, while the broader&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits">nucleic acid extraction kits hub</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits category</a> link to chromosomal DNA workflows.&nbsp;
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Miniprep Kits

MSDS - D4208T

MSDS - D4209/D4210/D4211/D4212

Protocol

ZymoPURE™ Plasmid Miniprep Kit

D4208T ZymoPURE Plasmid Miniprep Kit by Zymo Research — 10 preps; 15-min protocol; up to 100 &micro;g yield; &le;1 EU/&micro;g endotoxin; 25 &micro;l elution; A260/A280 &ge;1.8; plasmids to ~200 kb.

Sample Volume

ZymoPURE™ Plasmid Miniprep Kit (10 Preps.)

ZymoPURE™ Plasmid Miniprep Kit (50 Preps.)

ZymoPURE™ Plasmid Miniprep Kit (100 Preps.)

ZymoPURE™ Plasmid Miniprep Kit (400 Preps.)

ZymoPURE Plasmid Miniprep Kit (800 Preps.)

MSDS

ZymoPURE™-96 Plasmid Miniprep Kit

D4214 by Zymo Research is the ZymoPURE™-96 Plasmid Miniprep Kit for processing 96 minipreps simultaneously in ≤60 minutes. Yields up to 100 µg transfection-grade plasmid (≤1 EU/µg, A260/A280 ≥1.8) per well in 125 µL from up to 5 mL E. coli culture. Kit supplies 2 × 96 preps.

Culture Input 

Recommended Sample Volume

ZymoPURE™-96 Plasmid Miniprep Kit (2 x 96 Preps.)

ZymoPURE™-96 Plasmid Miniprep Kit (4 x 96 Preps.)

ZymoPURE 96 Starter Package (4 x 96 preps + 1 EZ-Vac 96 Vacuum Manifold)

Zymoprep™ Yeast Plasmid Miniprep I

D2001 by Zymo Research is the Zymoprep™ Yeast Plasmid Miniprep I, a 100-prep precipitation-based kit for isolating plasmid DNA from S. cerevisiae, C. albicans, S. pombe, and other Zymolyase-susceptible yeasts. Processes ≤1.5 ml culture per prep; elutes ≥35 µl with yields of 0.01–0.3 µg for 2-micron plasmids. DNA is ready for PCR, transformation, and hybridization. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, Eurofins, and many other institutes across the USA.

Zymoprep™ Yeast Plasmid Miniprep I (100 Preps.)

Solution 1 Digestion Buffer (15 ml)

Solution 2 Lysis Buffer (15 ml)

Solution 3 Neutralizing Buffer (15 ml)

Zymoprep™ Yeast Plasmid Miniprep II

D2004 by Zymo Research — Zymoprep Yeast Plasmid Miniprep II, 50 preps. Isolates plasmid DNA from ≤1.5 mL yeast culture using Zymolyase digestion and Zymo-Spin I silica columns; elution ≥10 µl; typical yield 0.01–0.3 ng. Compatible with S. cerevisiae, C. albicans, S. pombe, and ~15 additional Zymolyase-susceptible genera. Yields ~5× more plasmid than Miniprep I.

Processing Volume

Sample Source

Zymoprep™ Yeast Plasmid Miniprep II (50 Preps.)

Solution 1 Digestion Buffer (10 ml)

Solution 1 Digestion Buffer (45 ml)

Solution 1 Digestion Buffer (90 ml)

Solution 2 Lysis Buffer (10 ml)

Solution 2 Lysis Buffer (45 ml)

Solution 2 Lysis Buffer (90 ml)

Solution 3 Neutralizing Buffer (180 ml)

Solution 3 Neutralizing Buffer (20 ml)

Solution 3 Neutralizing Buffer (90 ml)

Zymoprep™-96 Yeast Plasmid Miniprep

D2005 by Zymo Research purifies plasmid DNA from S. cerevisiae, C. albicans, and S. pombe in a 96-well spin-column format, delivering 0.01–0.3 µg per well in ≥10 µl eluate with no glass beads or phenol required. The 2 × 96 prep kit recovers ~5-fold more plasmid than Zymoprep I and integrates with standard microplate centrifuges.

Zymoprep™-96 Yeast Plasmid Miniprep (2 x 96 Preps.)

Zymoprep™-96 Yeast Plasmid Miniprep (4 x 96 Preps.)

Zymoprep-96 Yeast Plasmid Miniprep (8 x 96 Preps.)

Zyppy Elution Buffer

D4036-5 by Zymo Research is the Zyppy Elution Buffer, composed of 10 mM Tris-HCl and 0.1 mM EDTA at pH 8.5, for eluting plasmid DNA from Zymo-Spin IIN columns in the Zyppy Plasmid Miniprep Kit (D4036). Available in six volumes from 5 ml to 100 ml for component replenishment. Compatible with all Zyppy Plasmid Miniprep Kit formats; room temperature storage. Made in USA by Zymo Research.

Zyppy™ Elution Buffer (100 ml)

Zyppy™ Elution Buffer (20 ml)

Zyppy™ Elution Buffer (30 ml)

Zyppy™ Elution Buffer (5 ml)

Zyppy™ Elution Buffer (60 ml)

Zyppy Wash Buffer (Concentrate)

Zymo Research D4036-4 Zyppy™ Wash Buffer concentrate. Add ethanol 4:1 before use; 400 µl working buffer per miniprep column. Final wash step in Zyppy Plasmid Miniprep protocol. Compatible with Zyppy Miniprep Kits (D4036, D4019, D4020, D4037). Available in 6 mL, 12 mL, 24 mL, and 48 mL concentrate sizes. Room temperature storage. Made in USA.

Zyppy™ Wash Buffer (Concentrate) (12 ml)

Zyppy™ Wash Buffer (Concentrate) (24 ml)

Zyppy™ Wash Buffer (Concentrate) (48 ml)

Zyppy™ Wash Buffer (Concentrate) (6 ml)

Zyppy-96 Plasmid Kit

D4042 by Zymo Research is the Zyppy™-96 Plasmid Kit in 4 × 96 prep format (384 total), featuring a pellet-free 45-minute protocol that processes a full 96-well plate of E. coli cultures directly—no centrifuge pellet step required. Each well yields up to 5 µg of plasmid DNA at A260/280 ≥1.8 in ≥30 µl, suitable for sequencing, restriction digestion, and CRISPR construct screening.

Zyppy™-96 Plasmid Kit (4 x 96 Preps.)

Zyppy™-96 Plasmid Kit (8 x 96 Preps.)

<h2>What Are Magnetic Bead Plasmid DNA Extraction Kits?</h2>
&nbsp;
Magnetic bead plasmid DNA kits use superparamagnetic nanoparticles, typically silica-coated or anion-exchange-functionalized, suspended in optimized binding buffers to capture plasmid DNA from bacterial lysate. After alkaline lysis of E. coli culture (0.5-5 mL per reaction), the beads bind DNA; an external magnetic field then immobilizes the beads so supernatant, RNA, and proteins are washed away without centrifugation. The purified DNA is eluted in water or low-salt buffer and is ready for downstream use. Throughput scales from single-tube manual processing to 96-well plate automation on KingFisher Duo, MagnetaPure 32, or ASSIST PLUS liquid handlers. Choose magnetic bead kits when your lab runs plasmid minipreps at scale (24+ samples/run) or when centrifuge-free automation is required.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>Magbio HighPrep Plasmid DNA Plus Kit for rapid, automated plasmid purification using high-binding paramagnetic particle technology.</li>
<li>Zyppy&trade;-96 Plasmid MagPrep Kit for scalable magnetic separation of high-purity vector constructs.</li>
<li>Zyppy-96 Plasmid MagBead Prep Kit for faster, column-free plasmid isolation.</li>
<li>Magnetic bead-based plasmid purification solutions compatible with high-throughput laboratory workflows.</li>
<li>Column-free plasmid DNA extraction systems that simplify processing and reduce hands-on time.</li>
<li>Products designed for efficient recovery of clean plasmid DNA for cloning, sequencing, and transfection applications.</li>
<li>Automation-friendly plasmid preparation kits suitable for 96-well and large-batch sample processing.</li>
</ul>
&nbsp;
<h2>How to Choose Magnetic Bead Plasmid Kits</h2>
&nbsp;
Automation Platform Compatibility
Bead-based kits are designed for specific magnetic rod or liquid-handler platforms. Confirm compatibility before ordering: NucleoMag Plasmid kits are validated for MagnetaPure and IsoPure Mini; MagJET kits are optimized for the KingFisher series; MagMAX Pro HT kits are designed for high-throughput centrifuge-free workflows. Using a kit outside its validated platform may require buffer volume adjustments and is not supported by the manufacturer.
Bead Type and DNA Binding Capacity
Silica-coated magnetic beads use chaotropic binding chemistry similar to spin columns; anion-exchange-functionalized beads offer higher selectivity for covalently closed circular (CCC/supercoiled) plasmid. Standard miniprep bead kits bind 5-20 ug from 1.5 mL culture; scaled protocols for 5 mL can yield up to 50 ug. Check binding capacity against your expected yield to avoid saturation.
High-Copy vs Low-Copy Plasmid
Most bead-based kits are validated for standard high-copy plasmids (pUC, pBR322 derivatives). Low-copy constructs such as fosmids or BACs require longer lysis times and may need protocol modifications; check the kit's validated plasmid types before purchasing for non-standard vectors.
Elution Volume and Concentration
Bead protocols typically elute in 50-100 uL. If downstream transfection requires high DNA concentration, choose kits that support low-volume elution (50 uL) or perform a secondary concentration step. Check that the eluate is compatible with your downstream assay buffer conditions.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Plasmid DNA purity from bead-based kits is evaluated by the A260/A280 ratio (target 1.8-2.0) and the A260/A230 ratio (&gt;1.8). Supercoiling percentage (measured by agarose gel densitometry) should exceed 80% for standard transfection applications. Automated 96-well bead workflows complete plasmid isolation in 30-60 minutes for a full plate, compared to 1-2 hours for centrifuge-based spin-column processing of the same number of samples. All kits are Research Use Only (RUO).
&nbsp;
<section class="mbp-contact-cta"></section>
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.

Mag Beads

MagBead

D4101 - MSDS

D4101-Protocol

D4101-AppNote-Hamilton

D4101-AppNote-Tecan

Zyppy-96 Plasmid MagBead Prep Kit

D4101 by Zymo Research: Zyppy™-96 Plasmid MagBead Prep Kit, 4×96 preps (384 total). Up to 5 µg/prep from 750 µl E. coli culture; ~60 min; centrifuge-free; ≥30 µl elution. Plasmids up to 25 kb. Compatible with robotic platforms and ZR-96 MagStand. Made in USA.

Zyppy™-96 Plasmid MagPrep Kit (4 x 96 Preps.)

Zyppy™-96 Plasmid MagPrep Kit (8 x 96 Preps.)

Zyppy™ Plasmid Miniprep Kit

D4019 by Zymo Research is a 100-prep plasmid miniprep kit using a pellet-free 8-minute direct lysis protocol. Inputs 600 µl E. coli overnight culture; yields up to 25 µg plasmid DNA (up to 25 kb) in ≥30 µl at A260/A280 ≥1.8 and ≤50 EU/µg endotoxin. Patented colored buffers provide visual lysis confirmation. Compatible with PCR, sequencing, cloning, and transfection.

Zyppy™ Plasmid Miniprep Kit (100 Preps.)

Zyppy™ Plasmid Miniprep Kit (400 Preps.)

Zyppy™ Plasmid Miniprep Kit (50 Preps.)

D4036 by Zymo Research is the Zyppy Plasmid Miniprep Kit (50 preps) — a pellet-free, 8-minute plasmid purification system isolating up to 25 µg of DNA from 600 µl of E. coli culture with A260/A280 ≥1.8 purity and ≤50 EU/µg endotoxin. Uses Zymo-Spin IIN columns; elution into ≥30 µl; handles plasmids up to 25 kb. Suitable for transfection, colony screening, sequencing, and cloning. Made in USA by Zymo Research.

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