Nest Scientific Consumables

Cell Culture Flasks

<h2 dir="ltr">What are Cell Culture Flasks?</h2>
 Cell culture flasks (T-flasks) are sterile, rectangular polystyrene vessels with a canted, angled neck designed to house adherent or suspension cell cultures in a CO₂ incubator. They are available in growth surface areas from T25 (25 cm&sup2;) to T225 (225 cm&sup2;), with either tissue culture&ndash;treated (TC-treated) surfaces for adherent cells or non-treated surfaces for suspension and microbial culture. Cap options include vented caps with 0.2 &micro;m hydrophobic membranes for CO₂ gas exchange, and plug seal caps for anaerobic or sealed transport.&nbsp; Choose the flask size based on the number of cells needed for downstream applications: T25 for seed stocks, T75 for routine maintenance, T175 and T225 for scale-up and protein production runs.
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<h2 dir="ltr">What you will find:</h2>
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<a href="cell-culture-flasks/treated-cell-culture-flasks" title="Treated Cell Culture Flasks" style="color: #236fa1;">Treated Cell Culture Flasks</a>: Vacuum-plasma modified surfaces that enhance hydrophilic interaction for uniform monolayer formation.
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<a href="cell-culture-flasks/non-treated-cell-culture-flasks" title="Non-Treated Cell Culture Flasks:">Non-Treated Cell Culture Flasks:</a> Naturally hydrophobic polystyrene designed specifically for hybridomas and non-adherent suspension cultures.
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<h2 dir="ltr"> How to Choose Cell Culture Flasks</h2>
 Size by Growth Area and Working Volume T25 flasks (25 cm&sup2;, working volume 5&ndash;10 mL) are used for seed stocks, small-scale experiments, and initial passages of new cell lines where preservation of limited cells is critical. T75 flasks (75 cm&sup2;, 15&ndash;25 mL) are the routine maintenance format for most adherent cell lines in research settings. T175 flasks (175 cm&sup2;, 35&ndash;50 mL) suit scale-up for protein production, viral vector manufacturing, and high-yield transient transfection. T225 flasks (225 cm&sup2;, 50&ndash;70 mL) provide maximum single-flask growth area before scale-up to multi-layer vessels. TC-Treated vs. Non-Treated Surface TC-treated flasks (vacuum plasma treated, contact angle &lt;10&deg;) are essential for adherent mammalian cell lines &mdash; HeLa, HEK293, CHO (adherent), Vero, A549, NIH 3T3, and primary cells, including fibroblasts, epithelial cells, and endothelial cells. Non-treated flasks maintain the original hydrophobic PS surface, preventing adherent growth &mdash; used for suspension CHO, Jurkat, K562, hematopoietic cells, hybridomas, Sf9/Hi5 insect cells, and bacterial culture. Canted vs. Straight Neck A canted (angled) neck on the flask allows the flask to rest at an angle when standing, keeping the growth surface slightly tilted during feeding and pipetting. This angled geometry improves access for serological pipettes and reduces the risk of contaminating the culture surface during media changes. All NEST Scientific T-flasks use a canted neck design. Cap Type and Gas Exchange Vented caps with 0.2 &micro;m hydrophobic membranes are the standard for CO₂ incubator use. The 0.2 &micro;m pore size blocks microbial contamination while allowing CO₂ and O₂ to equilibrate freely, maintaining medium pH through the bicarbonate buffering system. Plug seal caps isolate the flask from the ambient atmosphere &mdash; used for short-term transport, sealed storage, and anaerobic culture applications. Some protocols require alternating between vent and plug seal during different culture phases. Sterility and Traceability All NEST Scientific cell culture flasks carry E-beam sterilization (ISO 11137 equivalent SAL 10⁻⁶), DNase/RNase-free and pyrogen-free lot certification, batch traceability codes, and are produced under ISO 9001:2015 and ISO 13485 quality management. Flasks are individually packaged in peelable Tyvek sleeves or resealable sterile bags. Each flask features two large labeling areas for cell line identification and passage number notation.
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<h2 dir="ltr">Compare Cell Culture Flask Sizes </h2>
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<ul>
<li>T25: Growth Area: 25 cm&sup2;; Working Volume: 5&ndash;10 mL; Typical Cell Yield (adherent): 5&ndash;8 &times; 10⁶; Best Application: Seed stock, new cell line setup.</li>
<li>T75: Growth Area: 75 cm&sup2;; Working Volume: 15&ndash;25 mL; Typical Cell Yield (adherent): 15&ndash;25 &times; 10⁶; Best Application: Routine passage and maintenance.</li>
<li>T175: Growth Area: 175 cm&sup2;; Working Volume: 35&ndash;50 mL; Typical Cell Yield (adherent): 35&ndash;60 &times; 10⁶; Best Application: Scale-up, transient transfection.</li>
<li>T225: Growth Area: 225 cm&sup2;; Working Volume: 50&ndash;70 mL; Typical Cell Yield (adherent): 50&ndash;80 &times; 10⁶; Best Application: High-yield expansion, virus production.</li>
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<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific cell culture flasks (T25 catalog 707001, T75 catalog 708001, T175 catalog 709001, T225 catalog 710001) are manufactured from 100% virgin polystyrene in an ISO 8 (Class 10,000) cleanroom. Vacuum plasma TC treatment creates a surface contact angle of &lt;10&deg; (hydrophilic) per NEST lot data, verified by water contact angle goniometry. Vented caps contain a hydrophobic 0.2 &micro;m PTFE membrane that passes the USP Particulate Standard for injectable preparations &mdash; confirmed free of fibers and particulates at lot release. Flask footprints are standard for incubator shelf spacing: T25 width 100 mm, T75 width 150 mm, T175/T225 width 167 mm. As of 2026, NEST Scientific flasks are stocked by MBP for same-case reorder by PO. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center. 
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Eliminate variables in the incubator to ensure absolute integrity in your results with MBP's Cell Culture Flasks! <a href="https://mbpinc.net/contact-us">Reach out to our team</a> today for volume-based pricing.

<h2 dir="ltr">What are TC-Treated Cell Culture Flasks?</h2>
 Tissue culture&ndash;treated (TC-treated) cell culture flasks are sterile polystyrene T-flasks whose inner growth surface has been modified by vacuum plasma treatment to introduce negatively charged, oxygen-containing functional groups (carboxyl, carbonyl, hydroxyl) that reduce the water contact angle from ~75&ndash;90&deg; (hydrophobic, untreated PS) to below 10&deg; (hydrophilic). This surface modification directly promotes cell attachment, spreading, and cytoskeletal organization in adherent mammalian cell lines. Without TC treatment, virtually all primary mammalian cells and established adherent lines, including HeLa, HEK293 (adherent), CHO-K1, Vero, A549, NIH 3T3, MDCK, and primary fibroblasts, fail to attach and undergo anoikis within 24&ndash;48 hours. TC-treated flasks are the correct default for any adherent mammalian cell line unless the experimental protocol explicitly specifies non-treated or specialized surfaces (ultra-low attachment, poly-D-lysine, collagen I).
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<h2 dir="ltr">What you will find:</h2>
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TC-Treated Surfaces: Vacuum-plasma modified polystyrene for maximum cellular adhesion and uniform growth kinetics.
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Gas Exchange Efficiency: Vented caps with 0.22&micro;m hydrophobic membranes ensure sterile CO2 exchange while preventing contamination.
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Scalable Geometry: Available in T25, T75, T175, and T225 formats to support both routine maintenance and high-yield production.
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Advanced Ergonomics: The angled neck design facilitates complete access for cell scrapers and serological pipettes, minimizing media waste.
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Optical Excellence: USP Class VI high-clarity material provides distortion-free microscopy for precise monitoring.
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Certified Purity: Validated RNase, DNase, and Pyrogen-free to ensure experimental data integrity.
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<h2 dir="ltr"> How to Choose TC-Treated Cell Culture Flasks</h2>
 Size by Growth Area and Workflow Stage T25 (25 cm&sup2;, 5&ndash;10 mL): seed stocks, low-passage critical cell lines, newly thawed cells, and experiments requiring fewer than ~8 &times; 10⁶ cells. T75 (75 cm&sup2;, 15&ndash;25 mL): the standard research-lab flask for routine weekly passage of most adherent cell lines; the most commonly stocked format. T175 (175 cm&sup2;, 35&ndash;50 mL): scale-up for transient transfection (lipofection, calcium phosphate, PEI), viral vector production (lentivirus, AAV), and protein expression experiments requiring 35&ndash;60 &times; 10⁶ adherent cells per flask. T225 (225 cm&sup2;, 50&ndash;70 mL): maximum single-flask density before transitioning to multi-layer BioFactory vessels; used for large-scale CHO adherent culture and high-yield antibody or virus production runs. Vented vs. Plug Seal Cap Vented caps with a 0.2 &micro;m hydrophobic PTFE membrane allow free CO₂/O₂ gas exchange &mdash; mandatory for cells buffered by the bicarbonate/CO₂ system in a CO₂ incubator (typically 5&ndash;10% CO₂). Plug seal caps create a sealed environment; use them for short-term transport between incubators, for HEPES-buffered media that do not require CO₂, or for anaerobic culture. Using a plug seal cap in a standard CO₂ incubator blocks gas exchange and acidifies the medium within 2&ndash;4 hours. Straight vs. Canted Neck Straight-neck flasks are the traditional geometry; canted (angled) neck flasks tilt the growth surface away from the neck, improving pipette access during media changes and cell harvesting. NEST Scientific uses a canted neck design across all flask sizes. Confirm your biosafety cabinet arm reach and pipette length before committing to one geometry in bulk. Lot-to-Lot Consistency For long-running experiments, drug screening, or validated bioprocess workflows, lot-to-lot TC treatment consistency is critical. NEST Scientific verifies contact angle on each manufacturing lot. Switching the flask lots mid-experiment can alter cell attachment density, proliferation rate, and morphology. Order sufficient flasks from a single lot for the duration of a defined experimental series. Cells That Require TC-Treated Surfaces HeLa, A549, Vero (BSC-1), HEK293 (adherent), CHO-K1 (monolayer), MDCK, NIH 3T3, MCF-7, U2OS, PC-3, HepG2, primary dermal fibroblasts, primary endothelial cells, primary epithelial cells, and most primary culture preparations require TC-treated surfaces. Cells that must NOT be cultured on TC-treated surfaces include Jurkat, K562, HL-60, suspension CHO-S, hybridomas, Sf9/Hi5 insect cells, and hematopoietic progenitor cells &mdash; use non-treated flasks for all of these. 
<h2 dir="ltr"> Specifications Context </h2>
 NEST Scientific TC-treated flasks are manufactured from 100% virgin polystyrene in ISO 8 (Class 10,000) cleanrooms. Vacuum plasma TC treatment is verified by water contact angle goniometry per manufacturing lot; contact angle &lt;10&deg; is the release criterion. E-beam sterilization achieves SAL 10⁻⁶; flasks are DNase/RNase-free and non-pyrogenic per lot certificate. Vented cap 0.2 &micro;m PTFE hydrophobic membrane is certified free of particles per USP Particulate Standard. Flask footprint dimensions comply with standard CO₂ incubator shelf spacing: T25 (100 mm width), T75 (150 mm), T175/T225 (167 mm). Double-layered Tyvek packaging maintains sterility until the point of use. NEST Scientific holds ISO 9001:2015, ISO 13485, and ISO 11137 certifications, and has FDA registration and CE marking. As of 2026, MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center.
 <a href="https://mbpinc.net/contact-us">Connect with the helpful team at MBP </a>today to secure a customized quote for your facility.

Treated Cell Culture Flasks

<h2 dir="ltr">What are Non-Treated Cell Culture Flasks?</h2>
 Non-treated cell culture flasks are sterile polystyrene T-flasks that retain the original hydrophobic, unmodified surface of the polystyrene substrate &mdash; no plasma, corona, or chemical surface treatment is applied. The hydrophobic surface minimizes cell attachment, making these flasks the correct vessel for cell types that grow in suspension or require minimal surface contact: suspension-adapted CHO, Jurkat, K562, HL-60, hematopoietic progenitors, hybridomas for monoclonal antibody production, insect cell lines (Sf9, Hi5), yeast, and bacteria. NEST Scientific non-treated flasks are manufactured from 100% virgin polystyrene in ISO 8 cleanrooms, E-beam sterilized to SAL 10⁻⁶, and available in T25, T75, T175, and T225 sizes with vented (0.2 &micro;m PTFE membrane) or plug seal caps. 
Use non-treated flasks exclusively for cell types that do not require surface attachment; placing adherent mammalian cell lines in a non-treated flask causes cell death within 24&ndash;48 hours. 
<h2 dir="ltr">What you will find:</h2>
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Suspension-Optimized Surface, like naturally hydrophobic polystyrene, ensures cells remain in suspension without surface interference.
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Scalable Formats precision-engineered in T25, T75, T175, and T225 sizes to fit various research scales.
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Aseptic Security with certified RNase-, DNase-, and Pyrogen-free to maintain the highest purity standards.
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Ergonomic Access with angled neck design facilitates seamless pipette access and reduces the risk of contamination during media changes.
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<h2 dir="ltr">How to Choose Non-Treated Cell Culture Flasks</h2>
 Application: Suspension Mammalian Cells Suspension-adapted cell lines &mdash; CHO-S, HEK293-F, Expi293, and HEK293-EBNA variants used in transient transfection &mdash; require non-treated flasks for static suspension culture between spinner flask passages or when the scale is too small for orbital shaker flasks. Cells must be agitated (shaken, pipetted, or rocked) periodically to prevent settling when in static non-treated flasks with volumes &gt;5 mL. Application: Hematopoietic and Immune Cells Non-adherent hematopoietic cell lines (Jurkat T-cells, K562 erythroleukemia, HL-60 promyelocytes, Raji B-cells) and primary hematopoietic cells (peripheral blood mononuclear cells (PBMCs), bone marrow progenitors) must be cultured in non-treated vessels. TC-treated surfaces activate integrin signaling in hematopoietic cells, inducing undesired differentiation and stress responses. Application: Hybridoma Culture Hybridoma cells for monoclonal antibody (mAb) production are non-adherent B-cell fusions that grow as suspension cultures. Non-treated T75 and T225 flasks, combined with HT-supplemented DMEM/HAT medium, are the standard vessels for hybridoma selection and expansion. Scale-up from T225 non-treated flasks typically progresses to spinner or hollow-fiber bioreactors. Application: Insect Cells Sf9 (Spodoptera frugiperda) and Hi5 (Trichoplusia ni) insect cells used in baculovirus expression vector systems (BEVS) require non-treated polystyrene for monolayer culture. On TC-treated surfaces, Sf9 cells attach so strongly that they are difficult to recover. Non-treated flasks allow Sf9 to loosely adhere and detach easily with tapping, or to grow as a loosely attached monolayer that tolerates trypsin-free passaging. Application: Microbial Culture Non-treated polystyrene T-flasks with plug seal caps can serve as culture vessels for E. coli, Bacillus subtilis, yeast (S. cerevisiae, P. pastoris), and other microorganisms where the large surface area per volume is needed, and standard Erlenmeyer flask orbital shaking is unavailable. Ventilated caps support aerobic culture; confirm that the vented 0.2 &micro;m membrane is compatible with your aeration requirements. Cap Selection for Non-Treated Flasks Vented caps (0.2 &micro;m PTFE membrane) are standard for suspension cultures in a CO₂ incubator. For microbial aerobic culture without a CO₂ incubator, vented caps with 0.2 &micro;m pore still provide adequate aeration. Plug seal caps are used for anaerobic culture, transport, and short-term storage.
<h2> Specifications Context</h2>
 NEST Scientific non-treated cell culture flasks are manufactured from 100% virgin polystyrene with no surface modification. Contact angle of non-treated PS surface is approximately 75&ndash;90&deg; (hydrophobic), confirmed by water contact angle measurement per NEST lot QC data. E-beam sterilized in ISO 8 cleanrooms to SAL 10⁻⁶; DNase-free, RNase-free, and non-pyrogenic per lot certificate. Vented caps use a 0.2 &micro;m hydrophobic PTFE membrane. As of 2026, NEST Scientific non-treated flasks are stocked by MBP in all four sizes (T25, T75, T175, T225) with vented and plug seal options, in case quantities for PO-based institutional ordering. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center. 
Elevate your suspension culture workflows. <a href="https://mbpinc.net/contact-us">Contact Molecular Biology Products Inc.</a> for your Non-Treated Cell Culture Flasks today!

Non-Treated Cell Culture Flasks

DataSheet

Product Literature

T150 U-Shaped Canted Neck Cell Culture Flask Specifications

T150 U-Shaped Canted Neck Cell Culture Flask

NEST Scientific T150 Cell Culture Flasks is a T150 U-shaped canted-neck cell culture flask with 150 cm² TC-treated growth surface, USP Class VI medical-grade polystyrene, plug seal cap, frosted marking area, and volume graduations. Sterile, 5/pk, 40/cs. For adherent mammalian cell expansion and subculture. Available with seal or vent cap in TC-treated or non-treated surface.

Manufacturer

150cm2 U-Shaped Canted Neck Cell Culture Flask, Plug Seal Cap, TC, sterile 5/pk, 40/cs

150cm2 U-Shaped Canted Neck Cell Culture Flask, Plug Seal Cap, Non-Treated, sterile 5/pk, 40/cs

150cm2 U-Shaped Canted Neck Cell Culture Flask, Vent Cap, Non-Treated, sterile 5/pk, 40/cs

150cm2 U-Shaped Canted Neck Cell Culture Flask, Vent Cap, TC, sterile 5/pk, 40/cs

<h2 dir="ltr">What are PCR and qPCR sealing films?</h2>
 PCR and qPCR sealing films are optically clear microplate seals applied to 96- and 384-well PCR plates to create an airtight barrier through every thermal cycle, preventing evaporation, condensation, and cross-well contamination while still letting fluorescence pass through to the qPCR detector. Most are pressure-sensitive adhesive (PSA) or heat-activated films built on polypropylene or polyester (PET) with low-autofluorescence chemistry. Standard formats fit SBS sheets of approximately 137 &times; 80 mm. Buy from this category whenever the seal sits on a plate that will be thermally cycled or read in real time, not stored or incubated under simple lids. 
<h2 dir="ltr">What you will find:</h2>
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<li dir="ltr" role="presentation">Optical-grade qPCR sealing films for fluorescence-based detection</li>
<li dir="ltr" role="presentation">Standard adhesive PCR films for routine thermal cycling</li>
<li dir="ltr" role="presentation">96-well microplate sealing films for standard PCR workflows</li>
<li dir="ltr" role="presentation">384-well compatible sealing films for high-throughput assays</li>
<li dir="ltr" role="presentation">High-temperature resistant films for -40&deg;C to 120&deg;C cycling</li>
<li dir="ltr" role="presentation">Low-evaporation sealing films for reaction stability</li>
<li dir="ltr" role="presentation">DNase/RNase-free films for contamination-sensitive assays</li>
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<h2 dir="ltr">How to choose PCR or qPCR sealing film</h2>
 Confirm true optical clarity if you read fluorescence Not every "clear" sealing film has enough optical clarity and low enough autofluorescence for real-time PCR; choose a film specifically rated for qPCR with high light transmittance and no autofluorescence so excitation and emission pass cleanly. Standard storage films can suppress the signal. Decide between adhesive (PSA) and heat-seal Pressure-sensitive adhesive films are applied with a roller or paddle at the bench and peel off; heat-seal films bond under a plate sealer at controlled temperature and pressure for the strongest barrier in high-throughput and automated qPCR. PSA suits flexible workflows; heat-seal suits volume. Match the plate, thermal cycler, and skirt Confirm the film fits 96- or 384-well plates from your supplier and seats properly under the heated lid of your thermal cycler &mdash; full-skirt, semi-skirt, and non-skirt plates have different sealing surfaces. Sheet size near 137 &times; 80 mm is the SBS norm. Check chemical and temperature resistance PCR plate sealers and thermal cyclers reach 105 &deg;C routinely, and some films are rated to 150 &deg;C; check that the film and adhesive withstand the cycler protocol and the master mix without softening, lifting at corners, or leaving residue after peeling.
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<h2 dir="ltr">Specifications context</h2>
 Quality qPCR films are defined by light transmittance (typically &ge; 90 %), absence of autofluorescence, evaporation rate (commonly &le; 3 %), thermal tolerance to about 105 &deg;C with high-temperature variants to 150 &deg;C, and a DNase/RNase- and human-DNA-free certification appropriate for amplification. Most are supplied as flat SBS-format sheets around 137 &times; 80 mm, packed 100 per box; some have perforated end tabs for one-handed peeling. As of 2026, they sit in a low-to-mid price tier, with heat-seal films at the higher end.&nbsp; 
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<a href="https://mbpinc.net/contact-us">Connect with the MBP team today</a>&nbsp;to find the perfect optical or standard seal for your amplification workflow. Browse the wider&nbsp;<a href="https://mbpinc.net/consumables/sealing-films">sealing films range </a>for post-PCR archiving and reorder today via <a href="https://mbpinc.net/quickorder">Quick Order</a>.

PCR & qPCR Film

ThermalSeal RTS Optically Clear, Silicone Adhesive

ThermalSeal RTS™ (TSS-RTQ-100) by MBP is an optically clear, silicone-adhesive plate seal for qPCR, long-term storage, protein crystallography, and HPLC. Direct alternative to Thermofisher 4311971 MicroAmp Optical Adhesive Film. Available in 100-seal (TSS-RTQ-100), 25-film (TSS-RTQ-25), and sterile 50-film (TSS-RTQS-50, &minus;80&deg;C to +105&deg;C) formats. Ships ambient.

Application

Temperature Range

Optical Adhesive Films For PCR, Storage & Crystallization - Replaces 4311971 by Thermofisher 100 Seals/Case

ThermalSeal RTS™, Optically Clear, Silicone Adhesive, 25 Sealing Films/Pack

ThermalSeal RTS™, Optically Clear, Silicone Adhesive, Sterile, qPCR, long-term storage, protein crystallography & HPLC, -80°C to +105°C, 50 Sealing Films/Pack

Vacuum Filtration

<h2 dir="ltr">What are Vacuum Bottle-Top Filter Systems?</h2>
 Vacuum bottle-top filter systems are single-use, sterile filtration assemblies consisting of a graduated polystyrene funnel with an integrated membrane filter bonded to a polystyrene receiver bottle, connected by a hose-port adaptor to a house vacuum line or benchtop vacuum pump. The applied negative pressure (&minus;50 to &minus;100 kPa) draws liquid through the membrane into the receiver bottle, which is sealed after filtration for immediate use or cold-room storage.&nbsp;
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<h2 dir="ltr">What you will find:</h2>
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50mL Vacuum Filtration Units for small-scale sterile sampling
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150mL Vacuum Filtration Systems for rapid reagent preparation
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250mL Filtration Units for routine buffer sterilization
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500mL Vacuum Filtration Cups for mid-scale media processing
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1000mL Large Volume Filtration for industrial-scale batching
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Sterile Bottle-Top Filters for secure aseptic transfer
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<h2 dir="ltr"> How to Choose Vacuum Filtration Systems</h2>
 PES vs. PVDF Membrane for Vacuum Filtration PES membranes provide low protein binding (~1&ndash;2 &micro;g/cm&sup2;), high flow rates, and broad aqueous-solution compatibility &mdash; the standard for routine DMEM, RPMI, HBSS, PBS, and supplement-containing media sterilization. PVDF membranes have slightly lower protein binding than PES and greater chemical resistance to mild organic solvents, mild acids, and alkaline solutions. Use PVDF when filtering protein-rich solutions (FBS, antibody preparations, conditioned medium with high protein content) or when mild organic solvents are present in the filter solution. Volume Selection 250 mL systems (50 mm diameter membrane, 50 mL funnel) suit small media aliquots, supplement solutions, and transfection reagent preparations. 500 mL systems (90 mm diameter membrane, 150 mL funnel) are the routine lab workhorse for standard 500 mL media bottle preparation and FBS filtration. 1,000 mL systems (90 mm diameter membrane, 350 mL funnel) handle full-liter media preparations and large-volume buffer sterilization. All NEST systems include a pre-attached, graduated polystyrene receiver bottle in the corresponding volume. Pore Size: 0.10 &micro;m vs. 0.22 &micro;m vs. 0.45 &micro;m 0.22 &micro;m is the sterilizing grade per USP &lt;1229.2&gt; and ICH Q9: retains all bacteria and fungi; used for routine cell culture media, PBS, and buffer preparation. 0.45 &micro;m is the clarification grade: used as a pre-filtration step before 0.22 &micro;m to remove large particulates from turbid or high-biomass samples (FBS, conditioned media, cell culture supernatants, crude lysates), extending 0.22 &micro;m filter throughput by 3&ndash;5&times; on difficult samples. 0.10 &micro;m is the mycoplasma-removal grade: required when preparing media for Mycoplasma-sensitive primary cell culture or when processing serum or supplementary reagents that may harbor Mycoplasma species. Note that 0.10 &micro;m filtration is substantially slower than 0.22 &micro;m due to the tighter membrane pore structure. Practical Tips for Maximum Throughput Pre-wet the membrane by briefly passing 5&ndash;10 mL of sterile water through before the sample &mdash; this wets the hydrophilic PES or PVDF membrane and reduces initial resistance. For FBS and other viscous solutions, pre-filter through 0.45 &micro;m before the 0.22 &micro;m step. Maintain a vacuum at &minus;50 to &minus;80 kPa; excessive vacuum (&gt; &minus;100 kPa) can shear fragile membrane pores and increase non-specific protein adsorption. Do not autoclave the assembled bottle-top filter &mdash; PES and PS components are not rated for autoclave sterilization. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific vacuum bottle-top filters are manufactured from polystyrene funnel and receiver bottle components with PES or PVDF membranes. PES membranes have documented low protein binding (~1&ndash;2 &micro;g/cm&sup2;) per NEST scientific USA FAQ. Membrane diameter: 250 mL volume &rarr; 50 mm; 500 mL and 1,000 mL &rarr; 90 mm. The hose connector design accommodates various standard vacuum hose diameters. NEST PES 0.22 &micro;m vacuum filters are described as comparable in performance to leading brands, including Corning Stericup, Thermo Fisher Scientific, and Sartorius, per NEST Scientific USA's product page. All NEST vacuum filters are sterile and non-pyrogenic per the lot certificate. NEST Scientific vacuum bottle-top filters are stocked by MBP in all core SKUs. 
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Because your research should move faster than a slow-dripping filter. <a href="https://mbpinc.net/contact-us" title="Contact the Molecular Biology Products Inc. team" style="color: #236fa1;">Contact the Molecular Biology Products Inc. team</a> for a quote before your deadlines catch up with you.

Datsheet

Vacuum Filtration Systems  (PES Membrane)

Vacuum Filtration Systems, PES, Sterile

NEST sterile PES vacuum filtration systems in 15 variants — five volumes (50–1000 mL) × three pore sizes (0.10, 0.22, 0.45 µm). Low protein binding, E-beam SAL 10⁻⁶, pyrogen/DNase/RNase-free. SKUs 347001–344003. 1/pk, 12/cs.

50 mL Vacuum Filtration, 0.10 μm, PES, Sterile, 1/pk, 12/cs

50 mL Vacuum Filtration, 0.22 μm, PES, Sterile,1/pk, 12/cs

50 mL Vacuum Filtration, 0.45 μm, PES, Sterile, 1/pk, 12/cs

150 mL Vacuum Filtration, 0.10 μm, PES, Sterile, 1/pk, 12/cs

150 mL Vacuum Filtration, 0.22 μm, PES, Sterile,1/pk, 12/cs

150 mL Vacuum Filtration, 0.45 μm, PES, Sterile, 1/pk, 12/cs

250 mL Vacuum Filtration, 0.10 μm, PES, Sterile, 1/pk, 12/cs

250 mL Vacuum Filtration, 0.22 μm, PES, Sterile, 1/pk, 12/cs

250 mL Vacuum Filtration, 0.45 μm, PES, Sterile, 1/pk, 12/cs

500 mL Vacuum Filtration, 0.10 μm, PES, Sterile, 1/pk, 12/cs

500 mL Vacuum Filtration, 0.22 μm, PES, Sterile, 1/pk, 12/cs

500 mL Vacuum Filtration, 0.45 μm, PES, Sterile, 1/pk, 12/cs

1000 mL Vacuum Filtration, 0.10 μm, PES, Sterile, 1/pk, 12/cs

1000 mL Vacuum Filtration, 0.22 μm, PES, Sterile, 1/pk, 12/cs

1000 mL Vacuum Filtration, 0.45 μm, PES, Sterile, 1/pk, 12/cs

Vacuum Filtration Systems (PVDF Membrane)

Vacuum Filtration Systems, PVDF, Sterile

NEST Scientific sterile PVDF vacuum filtration systems: 5 volumes (50/150/250/500/1,000 mL) × 3 pore sizes (0.1/0.22/0.45 µm) = 15 SKUs. E-beam SAL 10⁻⁶, DNase/RNase/endotoxin-free, low-protein-binding. SKU suffix: -01=0.1 µm, -02=0.22 µm, -03=0.45 µm.

50 mL Vacuum Filtration, 0.10 μm, PVDF, Sterile, 1/pk, 12/cs

50 mL Vacuum Filtration, 0.22 μm, PVDF, Sterile, 1/pk, 12/cs

50 mL Vacuum Filtration, 0.45 μm, PVDF, Sterile, 1/pk, 12/cs

150 mL Vacuum Filtration, 0.10 μm, PVDF, Sterile, 1/pk, 12/cs

150 mL Vacuum Filtration, 0.22 μm, PVDF, Sterile, 1/pk, 12/cs

150 mL Vacuum Filtration, 0.45 μm, PVDF, Sterile, 1/pk, 12/cs

250 mL Vacuum Filtration, 0.10 μm, PVDF, Sterile, 1/pk, 12/cs

250 mL Vacuum Filtration, 0.22 μm, PVDF, Sterile, 1/pk, 12/cs

250 mL Vacuum Filtration, 0.45 μm, PVDF, Sterile, 1/pk, 12/cs

500 mL Vacuum Filtration, 0.10 μm, PVDF, Sterile, 1/pk, 12/cs

500 mL Vacuum Filtration, 0.22 μm, PVDF, Sterile, 1/pk, 12/cs

500 mL Vacuum Filtration, 0.45 μm, PVDF, Sterile, 1/pk, 12/cs

1000 mL Vacuum Filtration, 0.10 μm, PVDF, Sterile, 1/pk, 12/cs

1000 mL Vacuum Filtration, 0.22 μm, PVDF, Sterile, 1/pk, 12/cs

1000 mL Vacuum Filtration, 0.45 μm, PVDF, Sterile, 1/pk, 12/cs

BioFactory Accessories

<h2 dir="ltr"> What are Accessories for NEST BioFactory&trade; Systems?</h2>
 NEST BioFactory&trade; accessories are the replacement caps, silicone tubing, vent filters, overcaps, and pre-assembled closed-system cap assemblies used to configure BioFactory units for closed aseptic liquid transfer, gas exchange management, and multi-unit serial connections in cGMP and research-scale bioprocess workflows. The accessory set enables the BioFactory narrow-mouth cap to function as a closed-system liquid transfer port &mdash; media, wash buffer, trypsin, and cell harvest supernatant are transferred in and out through the silicone tubing connection without opening the vessel. Accessories for the BioFactory are required when operating the BioFactory as a closed system; for simple open-pour workflows using wide-mouth caps, standard vent and plug seal caps are the only accessories needed.
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<h2 dir="ltr">What you will find:</h2>
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BioFactory media transfer caps
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Sterile tubing and connector sets
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Fluid transfer adapters for cell culture vessels
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BioFactory bottle caps and closures
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Sterile quick-connect systems
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Liquid handling accessories
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Vessel handling and support tools
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Closed-system connectors for aseptic processing
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<h2 dir="ltr"> Key BioFactory Accessory Categories</h2>
 BioFactory Cap with Silicone Tube (CPC or Luer Connector) The BioFactory cap with silicone tube is the primary closed-transfer accessory: an adaptor cap that fits the narrow-mouth port of the BioFactory unit, pre-assembled with 80 cm of silicone tubing and a terminal connector &mdash; either a male CPC (clamped pressure connector / quick-connect) or a standard Luer lock &mdash; for coupling to peristaltic pumps, other BioFactory units, centrifuge tubes, or PETG media bottle assemblies. Two tubing I.D. &times; O.D. configurations are available: 1/4" I.D. &times; 3/8" O.D. and 3/8" I.D. &times; 5/8" O.D., covering standard peristaltic pump head bore sizes. The female sealing cap included with each assembly caps the connector between uses, maintaining sterility. Overcaps: Vented and Solid Overcaps are secondary dust and contamination barriers placed directly on top of the BioFactory adaptor caps during culture incubation. The vented overcap contains a 0.22 &micro;m hydrophobic breathable membrane, allowing gas exchange through the cap stack while blocking particles and microbial contamination &mdash; used during active shaker or orbital incubation when the unit's primary cap is an adaptor cap rather than a vented cap. The solid overcap provides an additional dust and contamination seal with no gas exchange &mdash; used for short-term storage or transport outside the incubator. SPT-50 Silicone Hose SPT-50 silicone hose (3/8" I.D. &times; 5/8" O.D., 50 inches / 15 meters) is the bulk tubing used to connect multiple BioFactory units in series, connect BioFactory ports to peristaltic pump heads, or extend the reach of the transfer cap assembly. SPT-50 tubing is manufactured from silicone, is individually wrapped and sterile, and passes dissolution testing per BPOG (BioPhorum Operations Group) criteria per NEST product documentation, supporting its use in biopharmaceutical production environments. Premium Accessory Package NEST offers a BioFactory Accessory Premium Package that includes 2 different adaptor caps, 1 hose clamp, 1 adaptor connector, 1 vent filter (50 mm), 1 additional hose clamp, and 15 m of SPT-50 silicone hose &mdash; all individually wrapped and sterile in a single kit. This package provides all components needed to set up a closed-system BioFactory media management workflow from a single kit, reducing the number of individual part orders required. Vent Filters for Gas Exchange Management For BioFactory units using adaptor caps rather than standard vented caps, 50 mm inline vent filters (0.22 &micro;m hydrophobic PTFE membrane) are installed on the air inlet/outlet tubing line to maintain sterile gas exchange during media addition, harvest, and incubation. The vent filter is rated for high flow rates without creating excessive back-pressure that would resist liquid movement through the system. Compatibility: Narrow-Mouth BioFactory Units Only All BioFactory accessory caps and tubing assemblies are designed exclusively for the narrow-mouth BioFactory configuration. Wide-mouth BioFactory units use standard wide-mouth vent and plug seal caps only; they are not compatible with accessory cap assemblies. Confirm that your BioFactory units have narrow-mouth ports before ordering the accessory cap set. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST BioFactory&trade; accessory silicone tubing and cap assemblies are manufactured from USP Class VI silicone (TPE/silicone) and polycarbonate or PP cap bodies, individually wrapped and sterile. All components have passed dissolution testing per BPOG dissolution test criteria conducted by a certified third-party laboratory per NEST Scientific documentation. The standard Luer head connections conform to ISO 594 / GB/T 1962 for reliable compatibility with standard laboratory fittings. Vent filters use a 0.22 &micro;m hydrophobic PTFE membrane; overcaps use a 0.22 &micro;m hydrophobic membrane. NEST BioFactory accessories are carried by MBP alongside BioFactory units. 
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Need the right BioFactory setup? <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact MBP today</a> for technical assistance.

Biofactory System Accessories

Vent Filter Specifications

Vent Filter，0.22 μm, Individually Wrapped, Sterile, 1/pk, 5/cs

NEST 0.22 µm Sterile Vent Filter: 0.22 &mu;m vent filter, 62 mm diameter, sterile, individually wrapped. Provides sterile gas exchange on NEST BioFactory systems and Erlenmeyer flask cap assemblies. 1/pk, 5/cs. Available in 62mm diameter (742001) and 56mm diameter (742011), both 0.22 µm, sterile, 1/pk, 5/cs.

Vent Filter，0.22 μm，62 mm diameter, Individually Wrapped, Sterile, 1/pk, 5/cs

Vent Filter，0.22 μm，56 mm diameter, Individually Wrapped, Sterile, 1/pk, 5/cs

NEST BioFactory Caps Specifications

Wide Mouth BioFactories, Individually Wrapped, Sterile, 1/pk, 10/cs

NEST Scientific BioFactory Caps is a 1-chamber Wide Mouth BioFactory with 647 cm² tissue-culture-treated growth surface. Sterile, individually wrapped, 1/pk, 10/cs. Plug or vent cap options available. For large-scale adherent cell culture, viral vector production, and cell expansion workflows. Available in seal or vent cap styles for wide-mouth or narrow-mouth BioFactory vessels.

Wide Mouth Plug Seal Caps for BioFactories, Individually Wrapped, Sterile, 1/pk, 10/cs

Wide Mouth Vent Caps for BioFactories, Individually Wrapped, Sterile, 1/pk, 10/cs

Narrow Mouth Plug Seal Caps for BioFactories, Individually Wrapped, Sterile, 1/pk, 10/cs

Narrow Mouth Vent caps for BioFactories, Individually Wrapped, Sterile, 1/pk, 10/cs

Erlenmeyer Flasks

<h2 dir="ltr">What are Cell Culture Erlenmeyer Flasks?</h2>
 Cell culture Erlenmeyer flasks (also called shake flasks or conical flasks) are single-use, sterile plastic vessels with a conical body, flat base, and narrow neck used for suspension cell culture on orbital shakers and incubator shakers, microbial culture, and media preparation. Unlike rectangular T-flasks designed for static monolayer culture, Erlenmeyer flasks are engineered for shaking: their wide conical base maximizes liquid surface area for oxygen transfer, while the narrow neck minimizes evaporation and contamination risk. Choose PC Erlenmeyer flasks for autoclavable reuse and high-temperature media preparation; choose PETG for single-use suspension culture and processes requiring BPA-free materials.
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<h2 dir="ltr">What you will find:</h2>
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<a href="erlenmeyer-flasks/petg-erlenmeyer-flasks">PETG Erlenmeyer flasks: </a>strong choice for cell culture applications that need excellent clarity, reliable handling, and convenient single-use performance. They are lightweight, durable, and ideal for shaker culture, media preparation, and general suspension cell workflows.
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<a href="erlenmeyer-flasks/pc-erlenmeyer-flasks">PC Erlenmeyer flasks: </a>built for applications that require greater strength and higher impact resistance. They are well-suited for demanding lab environments, including vigorous shaking, transport, and workflows where added durability is important.
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<li dir="ltr" aria-level="1"><a href="https://mbpinc.net/consumables/cell-culture/erlenmeyer-flasks/erlenmeyer-flask-accessories">Erlenmeyer Flask Accessories: </a>Includes bi-directional, inverted, and multifunctional transfer caps, plus silicon tube assemblies for 2 L, 3 L, 3 L wide-mouth, and 5 L high-efficiency flasks, supporting sterile transfer and closed-system workflows.</li>
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<h2 dir="ltr"> How to Choose Erlenmeyer Flasks for Cell Culture</h2>
 PC vs. PETG Material PC (polycarbonate) Erlenmeyer flasks are autoclavable at 121 &deg;C (typically 5&ndash;10 cycles), shatter-proof, crystal-clear, and rated from &minus;135 &deg;C to +135 &deg;C. PC is ideal for labs that sterilize media or wash in-house. PC contains BPA. PETG Erlenmeyer flasks are single-use, BPA-free, rated from &minus;80 &deg;C to +60 &deg;C, and cannot be autoclaved. They provide a contamination-free, cleaning-free alternative to glass and PC for high-throughput suspension culture operations. Flat Base vs. Baffled Base Flat-base Erlenmeyer flasks provide gentler swirling for slow-growth cell lines and media mixing. Baffled-base flasks have three or four angled internal baffles that interrupt the swirling flow, increasing turbulence, raising dissolved oxygen (DO) by up to 2&times; compared to flat-base flasks at the same orbital speed, and reducing cell sedimentation. Baffled flasks are the standard for CHO-S, HEK293-F, Sf9, microbial, and yeast cultures requiring high oxygen transfer rates. NEST Scientific offers baffled and flat-base versions in both PC and PETG across all core volumes. Vented vs. Plug Seal Cap Vented caps contain a 0.22 &micro;m hydrophobic PTFE membrane that allows free O₂/CO₂ gas exchange while excluding microbial contamination. The NEST vented cap features an oversized vent area that improves gas exchange beyond standard-size membrane caps, supporting oxygen-demanding suspension cultures at higher cell densities. Plug seal caps create a fully sealed environment for media storage, static incubation, or transport outside the incubator. Always use vented caps for actively growing suspension cultures on an orbital shaker. Volume and Fill Ratio Erlenmeyer flasks should be filled to no more than 20&ndash;25% of the nominal flask volume for suspension shaker culture to maintain adequate headspace for oxygen transfer. A 500 mL flask running at 20% fill holds 100 mL of culture; a 1,000 mL flask holds 200 mL. Overfilling reduces oxygen transfer efficiency and creates foam that blocks the vented cap membrane. For media storage (no shaking), the fill volume can approach 80% of nominal. Shaker Compatibility NEST Scientific Erlenmeyer flasks (125 mL&ndash;3,000 mL) are compatible with standard laboratory orbital shakers, including those from Infors HT, New Brunswick Scientific (Innova), and Thermo Scientific MaxQ. Confirm flask neck O.D. and base diameter against your shaker's flask holder clips or clamp system before ordering large quantities. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific PC Erlenmeyer flasks are manufactured from USP Class VI medical-grade polycarbonate, gamma sterilized per ISO 11137 to SAL 10⁻⁶, and individually wrapped in sterile packaging. PETG Erlenmeyer flasks are E-beam sterilized and certified DNase/RNase-free, pyrogen-free per lot. Vented cap PTFE membranes are 0.22 &micro;m with an oversized vent area that increases gas exchange efficiency vs. standard-diameter membrane caps. NEST Scientific is ISO 9001:2015 and ISO 13485 certified with FDA registration. Flask catalog sizes extend to high-efficiency 2 L and 5 L PC formats (NEST High Efficiency Erlenmeyer Flask line) for production-scale suspension culture. MBP stocks the NEST Scientific Erlenmeyer flask line across all major volumes and configurations. 
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Support accurate mixing and culturing with premium Erlenmeyer flasks designed for modern laboratories. <a href="https://www.mbpinc.net/contact-us" title="Contact the MBP Team" style="color: #236fa1; text-decoration: underline;">Contact the MBP team</a>&nbsp;for a formal quote.

Flyer

Wide-mouth High Efficient Erlenmeyer Flask Closed System

The Wide-mouth High Efficient Erlenmeyer Flask Closed System by NEST Scientific is a sterile pre-assembled 3 L wide-mouth flask and bi-directional transfer cap bundle (1/pk, 4/cs). Available as C10322-AZB050A (TPE 50 cm 1/8&#34; ID, &#934;42 mm vent filter), C10323-BZC050A (TPE 50 cm 1/4&#34; ID, &#934;24 mm vent filter), and C20322-AZB050A (5 L with baffles, TPE 50 cm 1/8&#34; ID, &#934;24 mm vent filter); E-beam SAL 10&#8315;&#8706;, ISO 13485.

3L Wide-mouth High Efficiency Erlenmeyer Flask with Bi-directional Transfer Cap with TPE Tube (50cm 1/8" ID 1/4" OD), Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 4/cs

3L Wide-mouth High Efficiency Erlenmeyer Flask with Bi-directional Transfer Cap with TPE Tube (50cm 1/4" ID 3/8" OD), Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 4/cs

3L Wide-mouth High Efficiency Erlenmeyer Flask with Baffles, with Bi-directional Transfer Cap with TPE Tube (50cm 1/8" ID 1/4" OD), Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 4/cs

Y Shape CPC Connector for Inner Dia #3/8 Hose, Individually Wrapped, Sterile, 1/pk, 5/cs

NEST Scientific 751001: sterile Y-shape CPC connector for 3/8" ID hose in single-use bioprocess fluid systems. Splits or combines two fluid lines in a closed system. Individually wrapped, 1/pk, 5/cs. Order at <a href="https://www.mbpinc.net/quickorder">mbpinc.net/quickorder</a>.

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