Buffers

<h2>What Are Zymo Research Buffers?</h2>
<p>Zymo Research buffers are formulation-specific reagents used at defined steps &mdash; lysis, binding, wash, and elution &mdash; in Zymo-Spin column-based purification protocols for DNA, RNA, and bisulfite-converted DNA. Each buffer is optimized for a specific kit family: DNA Wash Buffer concentrate (requires ethanol addition before use) removes salts and contaminants before elution from Zymo-Spin silica columns; DNA Elution Buffer (10 mM Tris, pH 8.5, 0.1 mM EDTA) releases purified DNA from the matrix; RNA Wash Buffer supports RNA extraction kits including Quick-RNA and Direct-zol lines; M-Elution Buffer is designed for eluting bisulfite-converted DNA in EZ DNA Methylation kits. Researchers typically order these separately when a single buffer component runs out before the rest of the kit, or when scaling up a protocol beyond the volume included.</p>
<p><strong>TL;DR:</strong> Order Zymo buffers individually to replenish a specific step in your existing Zymo workflow without replacing the full kit.</p>
<h2>How to Choose Zymo Research Buffers</h2>
<h3>Match the buffer to your kit family</h3>
<p>Every Zymo buffer is paired to a specific kit line. DNA Wash Buffer and DNA/RNA Wash Buffer concentrate are used in DNA Clean &amp; Concentrator, Quick-DNA, and Zymoclean Gel DNA Recovery kits. RNA Wash Buffer pairs with Quick-RNA kits. ZymoBIOMICS DNA Wash Buffer 1 and Wash Buffer 2 are specific to the ZymoBIOMICS microbiomics line. Substituting a buffer from a different kit family can compromise yield or purity.</p>
<h3>Confirm ethanol addition requirements before use</h3>
<p>Several Zymo wash buffer concentrates require addition of 96-100% ethanol to the bottle before first use &mdash; the label states the exact volume. DNA Wash Buffer concentrate, DNA/RNA Wash Buffer concentrate, and Urine DNA Wash Buffer concentrate all require this step. Pre-made (non-concentrate) versions such as RNA Wash Buffer are supplied ready to use.</p>
<h3>Select the right volume for your throughput</h3>
<p>Zymo buffers are available in sizes from 6 ml (single-experiment replenishment) up to 500 ml (Buffer P1 Red, for high-volume plasmid prep labs). For labs processing more than 50 samples per week, ordering the largest available size reduces per-sample cost and the frequency of reordering.</p>
<h3>Choose elution buffer chemistry based on downstream application</h3>
<p>DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA) is the standard for most PCR and sequencing applications. For applications sensitive to EDTA &mdash; such as some restriction enzyme digests or direct use with divalent cation-dependent enzymes &mdash; eluting in molecular-grade water or a Tris-only buffer is preferable. M-Elution Buffer is specifically formulated for bisulfite-converted DNA and should not be substituted with standard elution buffer in methylation workflows.</p>
<h3>Verify research-use-only status</h3>
<p>All Zymo Research buffers sold through MBP are for research use only (RUO). They are not cleared for in vitro diagnostic use. For clinical or diagnostic laboratory applications, confirm regulatory status with your institutional compliance officer before use.</p>
<h2>Compare Zymo Buffer Types</h2>
<table>
<thead>
<tr>
<th>Buffer Type</th>
<th>Primary Application</th>
<th>Kit Family</th>
<th>Format Available</th>
</tr>
</thead>
<tbody>
<tr>
<td>DNA Wash Buffer (concentrate)</td>
<td>Salt removal before DNA elution</td>
<td>DNA Clean &amp; Concentrator, Quick-DNA</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA/RNA Wash Buffer (concentrate)</td>
<td>Salt removal for dual DNA/RNA preps</td>
<td>Quick-DNA/RNA kits</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA)</td>
<td>DNA release from silica column</td>
<td>All Zymo DNA purification</td>
<td>16 ml &ndash; available sizes vary</td>
</tr>
<tr>
<td>RNA Wash Buffer</td>
<td>RNA purification wash step</td>
<td>Quick-RNA kits</td>
<td>Ready-to-use</td>
</tr>
<tr>
<td>M-Elution Buffer</td>
<td>Elution of bisulfite-converted DNA</td>
<td>EZ DNA Methylation kit families</td>
<td>Small volume</td>
</tr>
<tr>
<td>ZymoBIOMICS DNA Wash Buffer 2</td>
<td>Metagenomic/microbiomic DNA cleanup</td>
<td>ZymoBIOMICS DNA kits</td>
<td>Sold separately</td>
</tr>
<tr>
<td>Buffer P1 Red</td>
<td>Alkaline lysis resuspension (plasmid prep)</td>
<td>Zyppy Plasmid Miniprep</td>
<td>10 ml &ndash; 500 ml</td>
</tr>
</tbody>
</table>
<h2>Specifications Context</h2>
<p>Zymo wash buffer concentrates are supplied at a defined fold-concentration (commonly 4x or 6x) and require ethanol addition to reach working strength &mdash; the exact ratio is printed on the bottle and in the kit protocol. Elution buffer volumes for Zymo-Spin columns typically range from 6 to 100 &micro;l per elution; larger elution volumes improve total yield at the cost of DNA concentration. For DNA elution from Zymo-Spin columns, the protocol recommends a minimum 1-minute contact time between elution buffer and the silica matrix to maximize recovery of fragments 10 kb and above. As of 2025, Zymo Research tests all buffer lots for performance consistency, with integrity guaranteed for up to one year from purchase date.</p>
<p>Browse the full <a href="https://www.notion.so/reagents">Zymo Research reagents collection at MBP</a> for kits and columns that use these buffers, or visit our <a href="https://www.notion.so/reagents/buffers">reagent buffers category</a> for non-Zymo buffer options including ABM and other brands. For volume orders or purchase orders, use the <a href="https://www.notion.so/quickorder">Quick Order</a> tool or <a href="https://www.notion.so/contact-us">contact us</a> directly &mdash; MBP is a registered vendor for institutional procurement systems.</p>
<p><em>Last updated: June 2025</em></p>
<p>&nbsp;</p>

Zymo Buffers

MSDS

ZymoPURE Wash 1 for ZymoPURE Plasmid Purification Kits

<p>D4200-5 by Zymo Research is ZymoPURE Wash 1, the ready-to-use first wash buffer (5 ml per prep) applied after DNA binding in ZymoPURE II Midiprep, Maxiprep, and Gigaprep workflows. No ethanol preparation needed. Removes initial-pass protein and RNA contaminants before ZymoPURE Wash 2 (D4200-6). Available in 20–500 ml sizes.</p>

Manufacturer

Shipping Condition

Storage Condition

ZymoPURE™ Wash 1 (20 ml)

ZymoPURE™ Wash 1 (320 ml)

ZymoPURE™ Wash 1 (410 ml)

ZymoPURE™ Wash Buffer 1 (500 ml)

ZymoPURE™ Wash 1 (55 ml)

ZymoPURE™ Elution Buffer for ZymoPURE Plasmid Purification Kits

<p>Zymo Research D4200-7 ZymoPURE™ Elution Buffer: 10 mM Tris-HCl pH 8.5, 0.1 mM EDTA. Compatible with ZymoPURE Miniprep, ZymoPURE II Midiprep/Maxiprep/Gigaprep, and ZymoPURE-Express Midiprep kits. Available in 1 mL, 6 mL, 12 mL, and 30 mL volumes. Room temperature storage. Non-hazardous. Made in USA.</p>

ZymoPURE™ Elution Buffer (1 ml)

ZymoPURE™ Elution Buffer (12 ml)

ZymoPURE™ Elution Buffer (30 ml)

ZymoPURE™ Elution Buffer (6 ml)

ZymoPURE™ P1 (Red) for ZymoPURE Plasmid Purification Kits

<p>D4200-1 by Zymo Research is ZymoPURE™ P1 (Red), the RNase A-containing resuspension buffer for ZymoPURE and ZymoPURE II Plasmid Purification Kits (Miniprep, Midiprep D4200/D4201, Maxiprep D4202/D4203). The red color visually confirms complete bacterial pellet resuspension before alkaline lysis. Available in 3 ml to 410 ml sizes. Produces transfection-grade plasmid DNA from E. coli. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, Eurofins, and many other institutes across the USA.</p>

ZymoPURE™ P1 (Red) (100 ml)

ZymoPURE™ P1 (Red) (13 ml)

ZymoPURE™ P1 (Red) (150 ml)

ZymoPURE™ P1 (Red) (210 ml)

ZymoPURE™ P1 (Red) (3 ml)

ZymoPURE™ P1 (Red) (410 ml)

ZymoPURE™ P2 (Blue) for ZymoPURE Plasmid Purification Kits

<p>D4200-2 by Zymo Research is the ZymoPURE™ P2 (Blue) alkaline lysis buffer for ZymoPURE II Midiprep, Maxiprep, and Gigaprep plasmid purification kits. Contains NaOH; 8 mL used per midiprep (up to 50 mL culture). Available in multiple volumes.</p>

ZymoPURE™ P2 (Blue) (100 ml)

ZymoPURE™ P2 (Blue) (13 ml)

ZymoPURE™ P2 (Blue) (150 ml)

ZymoPURE™ P2 (Blue) (210 ml)

ZymoPURE™ P2 (Blue) (3 ml)

ZymoPURE™ P2 (Blue) (410 ml)

ZymoPURE™ P3 (Yellow) for ZymoPURE Plasmid Purification Kits

<p>The D4200-3 by Zymo Research is ZymoPURE P3, the yellow neutralization buffer for ZymoPURE Plasmid Purification Kits (Miniprep, Midiprep II, Maxiprep II). Used as Step 3 of the alkaline lysis workflow, P3 neutralizes the lysate and co-precipitates chromosomal DNA and proteins before column capture. Available in six sizes from 3 ml to 410 ml for high-volume replenishment.</p>

ZymoPURE™ P3 (Yellow) (100 ml)

ZymoPURE™ P3 (Yellow) (13 ml)

ZymoPURE™ P3 (Yellow) (150 ml)

ZymoPURE™ P3 (Yellow) (210 ml)

ZymoPURE™ P3 (Yellow) (3 ml)

ZymoPURE™ P3 (Yellow) (410 ml)

ZymoPURE™ Wash 2 (Concentrate) for ZymoPURE Plasmid Purification Kits

<p>D4200-6 by Zymo Research is the ZymoPURE Wash 2 concentrate for secondary and tertiary wash steps in ZymoPURE Plasmid Purification Kit workflows. Add 107 ml of 95–100% ethanol to the 28 ml concentrate before use; apply 5 ml × 2 per prep. Compatible with ZymoPURE II Midiprep, Maxiprep, and Gigaprep kits. Sold as standalone refill.</p>

ZymoPURE™ Wash 2 (Concentrate) (10 ml)

ZymoPURE™ Wash 2 (Concentrate) (12 ml)

ZymoPURE™ Wash 2 (Concentrate) (23 ml)

ZymoPURE™ Wash 2 (Concentrate) (28 ml)

g-DNA Wash Buffer

<p>D3004-2 by Zymo Research is a genomic DNA wash buffer for the final column wash step in Zymo Research gDNA purification kits, removing residual salts and PCR inhibitors before elution. Available in multiple volumes (50–400 ml) for labs with varying throughput.</p>