Buffers

<h2>What Are Zymo Research Buffers?</h2>
<p>Zymo Research buffers are formulation-specific reagents used at defined steps &mdash; lysis, binding, wash, and elution &mdash; in Zymo-Spin column-based purification protocols for DNA, RNA, and bisulfite-converted DNA. Each buffer is optimized for a specific kit family: DNA Wash Buffer concentrate (requires ethanol addition before use) removes salts and contaminants before elution from Zymo-Spin silica columns; DNA Elution Buffer (10 mM Tris, pH 8.5, 0.1 mM EDTA) releases purified DNA from the matrix; RNA Wash Buffer supports RNA extraction kits including Quick-RNA and Direct-zol lines; M-Elution Buffer is designed for eluting bisulfite-converted DNA in EZ DNA Methylation kits. Researchers typically order these separately when a single buffer component runs out before the rest of the kit, or when scaling up a protocol beyond the volume included.</p>
<p><strong>TL;DR:</strong> Order Zymo buffers individually to replenish a specific step in your existing Zymo workflow without replacing the full kit.</p>
<h2>How to Choose Zymo Research Buffers</h2>
<h3>Match the buffer to your kit family</h3>
<p>Every Zymo buffer is paired to a specific kit line. DNA Wash Buffer and DNA/RNA Wash Buffer concentrate are used in DNA Clean &amp; Concentrator, Quick-DNA, and Zymoclean Gel DNA Recovery kits. RNA Wash Buffer pairs with Quick-RNA kits. ZymoBIOMICS DNA Wash Buffer 1 and Wash Buffer 2 are specific to the ZymoBIOMICS microbiomics line. Substituting a buffer from a different kit family can compromise yield or purity.</p>
<h3>Confirm ethanol addition requirements before use</h3>
<p>Several Zymo wash buffer concentrates require addition of 96-100% ethanol to the bottle before first use &mdash; the label states the exact volume. DNA Wash Buffer concentrate, DNA/RNA Wash Buffer concentrate, and Urine DNA Wash Buffer concentrate all require this step. Pre-made (non-concentrate) versions such as RNA Wash Buffer are supplied ready to use.</p>
<h3>Select the right volume for your throughput</h3>
<p>Zymo buffers are available in sizes from 6 ml (single-experiment replenishment) up to 500 ml (Buffer P1 Red, for high-volume plasmid prep labs). For labs processing more than 50 samples per week, ordering the largest available size reduces per-sample cost and the frequency of reordering.</p>
<h3>Choose elution buffer chemistry based on downstream application</h3>
<p>DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA) is the standard for most PCR and sequencing applications. For applications sensitive to EDTA &mdash; such as some restriction enzyme digests or direct use with divalent cation-dependent enzymes &mdash; eluting in molecular-grade water or a Tris-only buffer is preferable. M-Elution Buffer is specifically formulated for bisulfite-converted DNA and should not be substituted with standard elution buffer in methylation workflows.</p>
<h3>Verify research-use-only status</h3>
<p>All Zymo Research buffers sold through MBP are for research use only (RUO). They are not cleared for in vitro diagnostic use. For clinical or diagnostic laboratory applications, confirm regulatory status with your institutional compliance officer before use.</p>
<h2>Compare Zymo Buffer Types</h2>
<table>
<thead>
<tr>
<th>Buffer Type</th>
<th>Primary Application</th>
<th>Kit Family</th>
<th>Format Available</th>
</tr>
</thead>
<tbody>
<tr>
<td>DNA Wash Buffer (concentrate)</td>
<td>Salt removal before DNA elution</td>
<td>DNA Clean &amp; Concentrator, Quick-DNA</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA/RNA Wash Buffer (concentrate)</td>
<td>Salt removal for dual DNA/RNA preps</td>
<td>Quick-DNA/RNA kits</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA)</td>
<td>DNA release from silica column</td>
<td>All Zymo DNA purification</td>
<td>16 ml &ndash; available sizes vary</td>
</tr>
<tr>
<td>RNA Wash Buffer</td>
<td>RNA purification wash step</td>
<td>Quick-RNA kits</td>
<td>Ready-to-use</td>
</tr>
<tr>
<td>M-Elution Buffer</td>
<td>Elution of bisulfite-converted DNA</td>
<td>EZ DNA Methylation kit families</td>
<td>Small volume</td>
</tr>
<tr>
<td>ZymoBIOMICS DNA Wash Buffer 2</td>
<td>Metagenomic/microbiomic DNA cleanup</td>
<td>ZymoBIOMICS DNA kits</td>
<td>Sold separately</td>
</tr>
<tr>
<td>Buffer P1 Red</td>
<td>Alkaline lysis resuspension (plasmid prep)</td>
<td>Zyppy Plasmid Miniprep</td>
<td>10 ml &ndash; 500 ml</td>
</tr>
</tbody>
</table>
<h2>Specifications Context</h2>
<p>Zymo wash buffer concentrates are supplied at a defined fold-concentration (commonly 4x or 6x) and require ethanol addition to reach working strength &mdash; the exact ratio is printed on the bottle and in the kit protocol. Elution buffer volumes for Zymo-Spin columns typically range from 6 to 100 &micro;l per elution; larger elution volumes improve total yield at the cost of DNA concentration. For DNA elution from Zymo-Spin columns, the protocol recommends a minimum 1-minute contact time between elution buffer and the silica matrix to maximize recovery of fragments 10 kb and above. As of 2025, Zymo Research tests all buffer lots for performance consistency, with integrity guaranteed for up to one year from purchase date.</p>
<p>Browse the full <a href="https://www.notion.so/reagents">Zymo Research reagents collection at MBP</a> for kits and columns that use these buffers, or visit our <a href="https://www.notion.so/reagents/buffers">reagent buffers category</a> for non-Zymo buffer options including ABM and other brands. For volume orders or purchase orders, use the <a href="https://www.notion.so/quickorder">Quick Order</a> tool or <a href="https://www.notion.so/contact-us">contact us</a> directly &mdash; MBP is a registered vendor for institutional procurement systems.</p>
<p><em>Last updated: June 2025</em></p>
<p>&nbsp;</p>

Zymo Buffers

MSDS

10X DSN Digestion Buffer (5 ml)

<p>10X DSN Digestion Buffer (5 ml) (E1020-2-5) by Zymo Research — 10× concentrated reaction buffer for use with PureRec Duplex-Specific Nuclease (DSN). Supports degradation of dsDNA and DNA in DNA-RNA hybrids. Made in California, USA.</p>

Manufacturer

Shipping Condition

Storage Condition

DataSheet

2X Digestion Buffer

<p>D3050-1 by Zymo Research is a 2X Proteinase K digestion buffer for DNA and RNA extractions. Compatible with Zymo Research extraction kits. Available in 5 mL and 20 mL sizes. Made in the USA.</p>

2X Digestion Buffer (20 ml)

2X Digestion Buffer (5 ml)

MSDS - F9001-1/F9001-5

MSDS - F9003

Protocol

5-Fluoroorotic Acid (5-FOA)

<p>F9001-1 by Zymo Research is 5-Fluoroorotic Acid (5-FOA), 1 g powder, ≥98% purity, MW 174 g/mol. The standard URA3 counter-selection agent for yeast genetics — used in plasmid shuffling, allelic replacement, plasmid curing, and two-hybrid screens in <em>Saccharomyces cerevisiae</em>. Store at −20°C.</p>

Format

Molecular Weight

Product Storage

Purity

Solubility

Structure

5-Fluoroorotic Acid (Powder) (1 g)

5-Fluoroorotic Acid (Powder) (5 g)

100X 5-Fluoroorotic Acid (Liquid) (10 ml)

BashingBead Buffer

<p>ZR BashingBead Buffer (D6001-3-150) by Zymo Research — 150 mL, room temperature stable, 750 µL/sample, for bead-beating lysis of fecal, soil, fungal, and bacterial samples.</p>

BashingBead Buffer (150 ml)

BashingBead Buffer (40 ml)

Buffer P1 (Red)

<p>D4027-1 by Zymo Research — Buffer P1 (Red), the bacterial cell resuspension buffer for the ZR Plasmid Miniprep - Classic Kit and ZR BAC DNA Miniprep Kit (D4048). Red color provides visual lysis monitoring when combined with P2 (Green). Room temperature storage. Available in 10 ml, 20 ml, 80 ml, 160 ml, and 500 ml formats.</p>

Buffer P1 (10 ml) (Red)

Buffer P1 (160 ml) (Red)

Buffer P1 (20 ml) (Red)

Buffer P1 (320 ml) (Red)

Buffer P1, 500 ml (Red)

Buffer P1 (80 ml) (Red)

Buffer P3 (Yellow)

<p>D4027-3-12 by Zymo Research is Buffer P3 (Yellow), the 12 ml neutralization buffer for the ZR Plasmid Miniprep - Classic Kit alkaline lysis workflow. Available individually in four volumes (12 ml, 50 ml, 220 ml, 440 ml) to replenish existing kits without replacing the full kit.</p>

Buffer P3 (12 ml) (Yellow)

Buffer P3 (220 ml) (Yellow)

Buffer P3 (440 ml) (Yellow)

Buffer P3 (50 ml) (Yellow)

Cell-free Recovery Buffer (20 ml)

<p>Cell-free Recovery Buffer (R1072-3-20) by Zymo Research — 20 ml replacement component for the Quick-cfDNA/cfRNA Serum &amp; Plasma Kit. Separates RNA from DNA into separate final eluates. Room temperature storage.</p>

DNA Digestion Buffer

<p>E1010-1-16 by Zymo Research is the DNA Digestion Buffer (16 mL), the 1x reaction buffer for DNase I (Zymo Research E1010 DNase I Set). Used for in-solution DNA digestion to prepare DNA-free RNA for RT-PCR, post-IVT template removal, and pre-sequencing cleanup. Inactivate after use by adding 5 mM EDTA and heating to 75°C for 10 minutes.</p>

DNA Digestion Buffer (16 ml)

DNA Digestion Buffer (4 ml)

DNA Elution Buffer

<p>D3004-4 by Zymo Research is a 4 ml ready-to-use solution of 10 mM Tris, pH 8.5, 0.1 mM EDTA—the standard buffer for eluting DNA from silica spin columns, 96-well plates, and magnetic bead systems. The EDTA component stabilizes eluted DNA by chelating DNase-activating cations, and the pH 8.5 formulation is compatible with PCR, ligation, restriction digestion, and Sanger sequencing without dilution.</p>

DNA Elution Buffer (1 ml)

DNA Elution Buffer (10 ml)

DNA Elution Buffer (16 ml)

DNA Elution Buffer (4 ml)

DNA Elution Buffer (50 ml)

DNA Pre-Wash Buffer

<p>D3004-5 by Zymo Research is the DNA Pre-Wash Buffer for removing protein contaminants from silica spin columns in Quick-DNA genomic DNA purification kits (D3024, D3020, D3067, and related formats). Available in four volumes: 15 ml, 30 ml, 50 ml, and 250 ml for high-throughput replenishment. Applied as an intermediate wash step before the primary DNA Wash Buffer; room temperature storage. Made in USA by Zymo Research.</p>

DNA Pre-Wash Buffer (15 ml)

DNA Pre-Wash Buffer (250 ml)

DNA Pre-Wash Buffer (30 ml)

DNA Pre-Wash Buffer (50 ml)

DNA Recovery Buffer (9 ml)

<p>DNA Recovery Buffer R2050-5-9 by Zymo Research — 9 ml buffer for use in the Quick-DNA/RNA Blood Tube Kit (Cat. R1151), removing contaminants from DNA and RNA extracted from whole blood preserved with DNA/RNA Shield™. Sold separately for buffer component replenishment. Order via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or contact <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.</p>

DNA/RNA Buffer HT (100 ml)

<p>R2150-1-100 by Zymo Research is the DNA/RNA Buffer HT (100 ml), a one-step lysis and binding reagent for high-throughput simultaneous DNA and RNA extraction using the Quick-DNA/RNA HT magnetic bead system. Compatible with 96-well and 24-well formats; eluates ready for NGS and RT-qPCR. Requires user addition of beta-mercaptoethanol before use.</p>

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