Buffers

<h2>What Are Zymo Research Buffers?</h2>
<p>Zymo Research buffers are formulation-specific reagents used at defined steps &mdash; lysis, binding, wash, and elution &mdash; in Zymo-Spin column-based purification protocols for DNA, RNA, and bisulfite-converted DNA. Each buffer is optimized for a specific kit family: DNA Wash Buffer concentrate (requires ethanol addition before use) removes salts and contaminants before elution from Zymo-Spin silica columns; DNA Elution Buffer (10 mM Tris, pH 8.5, 0.1 mM EDTA) releases purified DNA from the matrix; RNA Wash Buffer supports RNA extraction kits including Quick-RNA and Direct-zol lines; M-Elution Buffer is designed for eluting bisulfite-converted DNA in EZ DNA Methylation kits. Researchers typically order these separately when a single buffer component runs out before the rest of the kit, or when scaling up a protocol beyond the volume included.</p>
<p><strong>TL;DR:</strong> Order Zymo buffers individually to replenish a specific step in your existing Zymo workflow without replacing the full kit.</p>
<h2>How to Choose Zymo Research Buffers</h2>
<h3>Match the buffer to your kit family</h3>
<p>Every Zymo buffer is paired to a specific kit line. DNA Wash Buffer and DNA/RNA Wash Buffer concentrate are used in DNA Clean &amp; Concentrator, Quick-DNA, and Zymoclean Gel DNA Recovery kits. RNA Wash Buffer pairs with Quick-RNA kits. ZymoBIOMICS DNA Wash Buffer 1 and Wash Buffer 2 are specific to the ZymoBIOMICS microbiomics line. Substituting a buffer from a different kit family can compromise yield or purity.</p>
<h3>Confirm ethanol addition requirements before use</h3>
<p>Several Zymo wash buffer concentrates require addition of 96-100% ethanol to the bottle before first use &mdash; the label states the exact volume. DNA Wash Buffer concentrate, DNA/RNA Wash Buffer concentrate, and Urine DNA Wash Buffer concentrate all require this step. Pre-made (non-concentrate) versions such as RNA Wash Buffer are supplied ready to use.</p>
<h3>Select the right volume for your throughput</h3>
<p>Zymo buffers are available in sizes from 6 ml (single-experiment replenishment) up to 500 ml (Buffer P1 Red, for high-volume plasmid prep labs). For labs processing more than 50 samples per week, ordering the largest available size reduces per-sample cost and the frequency of reordering.</p>
<h3>Choose elution buffer chemistry based on downstream application</h3>
<p>DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA) is the standard for most PCR and sequencing applications. For applications sensitive to EDTA &mdash; such as some restriction enzyme digests or direct use with divalent cation-dependent enzymes &mdash; eluting in molecular-grade water or a Tris-only buffer is preferable. M-Elution Buffer is specifically formulated for bisulfite-converted DNA and should not be substituted with standard elution buffer in methylation workflows.</p>
<h3>Verify research-use-only status</h3>
<p>All Zymo Research buffers sold through MBP are for research use only (RUO). They are not cleared for in vitro diagnostic use. For clinical or diagnostic laboratory applications, confirm regulatory status with your institutional compliance officer before use.</p>
<h2>Compare Zymo Buffer Types</h2>
<table>
<thead>
<tr>
<th>Buffer Type</th>
<th>Primary Application</th>
<th>Kit Family</th>
<th>Format Available</th>
</tr>
</thead>
<tbody>
<tr>
<td>DNA Wash Buffer (concentrate)</td>
<td>Salt removal before DNA elution</td>
<td>DNA Clean &amp; Concentrator, Quick-DNA</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA/RNA Wash Buffer (concentrate)</td>
<td>Salt removal for dual DNA/RNA preps</td>
<td>Quick-DNA/RNA kits</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA)</td>
<td>DNA release from silica column</td>
<td>All Zymo DNA purification</td>
<td>16 ml &ndash; available sizes vary</td>
</tr>
<tr>
<td>RNA Wash Buffer</td>
<td>RNA purification wash step</td>
<td>Quick-RNA kits</td>
<td>Ready-to-use</td>
</tr>
<tr>
<td>M-Elution Buffer</td>
<td>Elution of bisulfite-converted DNA</td>
<td>EZ DNA Methylation kit families</td>
<td>Small volume</td>
</tr>
<tr>
<td>ZymoBIOMICS DNA Wash Buffer 2</td>
<td>Metagenomic/microbiomic DNA cleanup</td>
<td>ZymoBIOMICS DNA kits</td>
<td>Sold separately</td>
</tr>
<tr>
<td>Buffer P1 Red</td>
<td>Alkaline lysis resuspension (plasmid prep)</td>
<td>Zyppy Plasmid Miniprep</td>
<td>10 ml &ndash; 500 ml</td>
</tr>
</tbody>
</table>
<h2>Specifications Context</h2>
<p>Zymo wash buffer concentrates are supplied at a defined fold-concentration (commonly 4x or 6x) and require ethanol addition to reach working strength &mdash; the exact ratio is printed on the bottle and in the kit protocol. Elution buffer volumes for Zymo-Spin columns typically range from 6 to 100 &micro;l per elution; larger elution volumes improve total yield at the cost of DNA concentration. For DNA elution from Zymo-Spin columns, the protocol recommends a minimum 1-minute contact time between elution buffer and the silica matrix to maximize recovery of fragments 10 kb and above. As of 2025, Zymo Research tests all buffer lots for performance consistency, with integrity guaranteed for up to one year from purchase date.</p>
<p>Browse the full <a href="https://www.notion.so/reagents">Zymo Research reagents collection at MBP</a> for kits and columns that use these buffers, or visit our <a href="https://www.notion.so/reagents/buffers">reagent buffers category</a> for non-Zymo buffer options including ABM and other brands. For volume orders or purchase orders, use the <a href="https://www.notion.so/quickorder">Quick Order</a> tool or <a href="https://www.notion.so/contact-us">contact us</a> directly &mdash; MBP is a registered vendor for institutional procurement systems.</p>
<p><em>Last updated: June 2025</em></p>
<p>&nbsp;</p>

Zymo Buffers

MSDS

Direct-zol DNA Wash

<p>Direct-zol DNA Wash replacement components by Zymo Research — available as DNA Wash 1 Concentrate (R2080-1-24, 24 ml), DNA Wash 2 (R2080-2-40, 40 ml), and DNA Elution Buffer (R2080-3-4, 4 ml) — are designed for use with Direct-zol™ DNA/RNA kits. Made in the USA. Suited to high-throughput nucleic acid purification labs requiring individual buffer replenishment.</p>

Manufacturer

Direct-zol™ DNA Wash 1 (Concentrate) (24 ml)

Direct-zol™ DNA Wash 2 (40 ml)

Direct-zol™ DNA Elution Buffer (4 ml)

Direct-zol™ RNA PreWash (Concentrate)

<p>Direct-zol™ RNA PreWash Concentrate R2050-2-160 by Zymo Research — 160 ml, room-temperature stable wash buffer for all Direct-zol RNA column-based kits. Removes TRI Reagent and phenol carryover from silica columns during RNA purification. Concentrate: requires preparation per kit protocol before first use. Also available in 40 ml (R2050-2-40). Order via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.</p>

Shipping Condition

Storage Condition

Direct-zol™ RNA PreWash (Concentrate) (160 ml)

Direct-zol™ RNA PreWash (Concentrate) (40 ml)

Endo-Wash Buffer

<p>D4036-3 by Zymo Research — Endo-Wash Buffer for endotoxin removal during plasmid DNA purification with the Zyppy Plasmid Miniprep and ZR Plasmid Miniprep - Classic kits. Room temperature storage. Available in 6 ml, 15 ml, 30 ml, 60 ml, 120 ml, 160 ml, and 240 ml formats. Yields ultra-pure, endotoxin-reduced plasmid DNA.</p>

Endo-Wash Buffer (120 ml)

Endo-Wash Buffer (15 ml)

Endo-Wash Buffer (160 ml)

Endo-Wash Buffer (240 ml)

Endo-Wash Buffer (30 ml)

Endo-Wash Buffer (6 ml)

Endo-Wash Buffer (60 ml)

Genomic Lysis Buffer

<p>D3004-1 by Zymo Research is the Genomic Lysis Buffer—a ready-to-use chaotropic reagent that lyses cells and whole blood, simultaneously degrades RNA, and conditions DNA for binding to Zymo Research silica spin columns and magnetic bead systems in a single step. Available in 50 mL to 250 mL volumes, it is compatible with the Quick-DNA Miniprep kit series and ZymoBIOMICS magnetic bead workflows.</p>

Genomic Lysis Buffer (100 ml)

Genomic Lysis Buffer (150 ml)

Genomic Lysis Buffer (2 x 100 ml)

Genomic Lysis Buffer (250 ml)

Genomic Lysis Buffer (50 ml)

MagBead DNA/RNA Wash 1 (Concentrate)

<p>MagBead DNA/RNA Wash 1 (Concentrate) by Zymo Research — wash buffer concentrate for use with Zymo Research magnetic bead-based DNA/RNA purification kits. Add isopropanol before use per kit protocol. Available in 30 ml (R2130-1-30) and 120 ml (R2130-1-120).</p>

MagBead DNA/RNA Wash 1 (Concentrate) (120 ml)

MagBead DNA/RNA Wash 1 (Concentrate) (30 ml)

MagBead DNA/RNA Wash 2 (Concentrate)

<p>MagBead DNA/RNA Wash 2 (Concentrate) (R2130-2-20) by Zymo Research is a 20 mL replacement wash buffer concentrate for magnetic bead&ndash;based nucleic acid purification. Prepare the working solution by adding 30 mL of isopropanol to the 20 mL concentrate before first use. During magnetic bead protocols, 500 &micro;L of working solution is applied per wash step to remove residual contaminants from bead-bound nucleic acids.</p>
<p>R2130-2-20 is a replacement component for the Quick-DNA/RNA&trade; MagBead Kit (R2130/R2131) and compatible Zymo MagBead systems. Store at room temperature. Order via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or contact <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.</p>

MagBead DNA/RNA Wash 2 (Concentrate) (20 ml)

MagBead DNA/RNA Wash 2 (Concentrate) (80 ml)

Pathogen DNA/RNA Buffer

<p>Pathogen DNA/RNA Buffer by Zymo Research — all-in-one lysis and binding reagent for nucleic acid extraction from viral, bacterial, and protozoan pathogens in vectors and tissue. Available as R1042-1-100 (100 ml) and R1042-1-50 (50 ml).</p>

Pathogen DNA/RNA Buffer (100 ml)

Pathogen DNA/RNA Buffer (50 ml)

Pathogen DNA/RNA Wash Buffer (Concentrate)

<p>Pathogen DNA/RNA Wash Buffer (Concentrate) by Zymo Research is a column wash reagent for Quick-DNA/RNA™ Pathogen Miniprep kits, used to isolate nucleic acids from mosquitoes, ticks, fleas, and mammalian/avian tissue. Available in 6 ml (R1042-2-6) and 48 ml (R1042-2-48) formats for workflow replenishment. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, and many more leading research institutes, serving labs across the United States, Canada, and internationally.</p>

Pathogen DNA/RNA Wash Buffer (Concentrate) (48 ml)

Pathogen DNA/RNA Wash Buffer (Concentrate) (6 ml)

DataSheet

Plasmid Wash Buffer

<p>D4027-4-12 by Zymo Research is a 12 ml Plasmid Wash Buffer concentrate for Zymo-Spin column wash steps in Zyppy and ZR Plasmid Miniprep workflows. Prepare with 95–100% ethanol before use; stored at room temperature. Sold as a standalone refill when the wash buffer component is depleted.</p>

Plasmid Wash Buffer (Concentrate) (12 ml)

Plasmid Wash Buffer (Concentrate) (24 ml)

Plasmid Wash Buffer (Concentrate) (48 ml)

Plasmid Wash Buffer (Concentrate) (6 ml)

D4077-1-MSDS

Quick-DNA MagBinding Buffer

<p>D4077-1 by Zymo Research — Quick-DNA MagBinding Buffer, available in 150 mL and 250 mL. The binding buffer component for Zymo Research Quick-DNA MagBead DNA purification workflows, enabling DNA capture onto magnetic beads in automated and manual extraction protocols. Research Use Only.</p>

Quick-DNA™ MagBinding Buffer (150 ml)

Quick-DNA™ MagBinding Buffer (250 ml)

Quick-cfDNA/cfRNA™ Binding Buffer (150 ml)

<p>Quick-cfDNA/cfRNA™ Binding Buffer R1072-2-150 by Zymo Research is a 150 ml standalone replacement component for the Quick-cfDNA/cfRNA™ Serum and Plasma Kit (R1072). Added 1:1 to proteinase K-digested serum or plasma, it conditions the sample for efficient cfDNA and cfRNA binding to the purification column. Stored at room temperature.</p>

Quick-cfDNA/cfRNA™ Digestion Buffer (150 ml)

<p>Quick-cfDNA/cfRNA™ Digestion Buffer (R1072-1-150) by Zymo Research is a 150 ml proteinase K digestion buffer used at a 1:1 ratio with serum or plasma in the Quick-cfDNA/cfRNA Serum & Plasma Kit (R1072). Applied at 37°C for 2 hours, it prepares liquid biopsy samples for cfDNA and cfRNA column-based purification.</p>

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