Brands

<h2 dir="ltr"> What Are Cas9 Enzymes?</h2>
 Cas9 is the catalytic core of the CRISPR/Cas9 editing system. Sourced from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9), recombinant Cas9 proteins are expressed and purified to high purity for direct use in ribonucleoprotein (RNP) complexes &mdash; currently the most widely recommended format for mammalian cell editing due to rapid activity onset and minimised off-target effects compared to plasmid-based delivery. Genome-editing performance depends on the appropriate nuclease format. Choose the Cas9 enzyme that aligns with your editing strategy.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
NLS-Tagged Cas9 and saCas9 Nucleases: Recombinant proteins of high purity designed for quick nuclear localization and strong initiation of double-strand breaks (DSBs) at specific genomic sites.
</li>
<li dir="ltr" aria-level="1">
Precision Cas9 Nickase (H840A): Tailored variants created for dual-nicking approaches to improve genomic stability and notably minimize off-target alterations.
</li>
<li dir="ltr" aria-level="1">
Lyophilized Nuclease Preparations: Nuclease-free, shelf-stable proteins that offer optimal adaptability for reconstitution and high-throughput automated CRISPR-related processes.
</li>
<li dir="ltr" aria-level="1">
Catalytically Inactive Null Mutants: "Dead" Cas9 (dCas9) proteins designed for DNA-binding research and specific epigenetic modification without changing the original sequence.
</li>
<li dir="ltr" aria-level="1">
Cas12a (asCpf1) Nucleases: Alternative CRISPR systems that focus on T-rich PAM sequences to broaden the range of spatial transcriptomics and targetable genomic areas.
</li>
<li dir="ltr" aria-level="1">
GFP-Tagged Cas9 Proteins: Fluorescently marked nucleases developed for real-time assessment of transfection efficacy and visual verification of cellular absorption.
</li>
<li dir="ltr" aria-level="1">
Endotoxin-Free Enzyme Formulations: Highly pure mixtures crucial for preserving cell viability in sensitive lentiviral applications and primary cell modifications.
</li>
</ul>
<h2 dir="ltr"> How to Choose a Cas9 Enzyme</h2>
 PAM Requirement Wild-type SpCas9 recognises any genomic locus flanked by an NGG protospacer adjacent motif (PAM), occurring roughly every 8&ndash;12 bp in mammalian genomes. SaCas9 requires NNGRRT, though engineered PAM variants (KKH, KRH, KRKH) expand targetable space. Off-Target Sensitivity High-fidelity Cas9 variants carry substitutions in the recognition lobe that reduce non-specific DNA contacts, lowering off-target cleavage while maintaining on-target activity comparable to wild-type SpCas9 &mdash; recommended for therapeutic cell engineering and base editing. Editing Mechanism Wild-type SpCas9 generates a blunt double-strand break repaired by NHEJ (indels for knockout) or HDR (precise insertions). Nickase (D10A) cuts only one strand for paired-nick HDR strategies with reduced off-target indels. dCas9 carries mutations in both nuclease domains, enabling CRISPRa/CRISPRi gene regulation without DNA cleavage. AAV Compatibility SaCas9, at approximately 1 kb smaller than SpCas9, fits a complete Cas9 + sgRNA expression cassette within the ~4.7 kb AAV packaging capacity &mdash; enabling single-vector in vivo delivery without split-vector complexity. Storage and Handling Recombinant Cas9 proteins are supplied for stability during shipping and short-term storage at &minus;20 &deg;C or &minus;80 &deg;C. Repeated freeze-thaw cycles reduce activity; aliquoting upon receipt is recommended.
<h2 dir="ltr"> Specifications Context</h2>
 An RNP is a pre-formed complex of recombinant Cas9 protein and sgRNA; once electroporated, it acts immediately and is degraded within hours, reducing off-target cleavage and avoiding immune stimulation associated with plasmid or mRNA delivery.&nbsp;Recombinant Cas9 in RNP format is validated across HEK293, HeLa, iPSCs, T cells, primary neurons, and hepatocytes, though electroporation parameters typically require cell-type-specific optimisation. Product offerings reflect MBP's catalogue as of mid-2026. 
&nbsp;
Reach out to the supportive <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">team at MBP Inc.</a> now to obtain a quotation for the perfect Cas9 enzyme for your study.

Cas9 Enzymes

DataSheet

MSDS

saCas9 Null Mutant NLS Protein

K147 by Applied Biological Materials is dsaCas9 — the catalytically inactive S. aureus Cas9 null mutant NLS protein (32.5 µg / 250 pmol / 25 µl). Binds DNA at NNGRRT PAM sites guided by saCas9-compatible sgRNA without DNA cleavage. For CRISPRi steric blockade, negative control in saCas9 editing experiments, and dCas9-based chromatin studies. Separate saCas9 sgRNA required.

Unit Quantity

Cas Type

Cas Origin

Cas Protein Marker

Concentration

Format General

Storage Buffer

Storage Condition

Caution

Shipping Condition

saCas9 Null Mutant Protein

K146 by Applied Biological Materials is catalytically dead saCas9 (dSaCas9) Null Mutant Protein. Both nuclease domains inactivated; sgRNA-guided DNA binding retained. For RNP editing negative controls, CRISPRi, base editing, and dSaCas9-effector fusion applications at NNGRRT PAM sites. Store at −20°C.

<h2>What are PCR Cleanup Kits?</h2>
&nbsp;
PCR cleanup kits remove residual PCR reaction components -- primers (&lt;100 bp), primer dimers, unincorporated dNTPs, DNA polymerase, buffer salts, and mineral oil - from PCR amplification products using silica-membrane spin columns and a bind-wash-elute protocol. The purified PCR amplicon (&gt;=100 bp) is recovered in 20-50 ul of water or elution buffer, free of inhibitors that would interfere with downstream steps, including Sanger sequencing, restriction enzyme digestion, ligation, NGS library preparation, and in vitro transcription. The procedure takes 5-10 minutes using a standard microcentrifuge with no organic solvents, precipitation, or centrifugation spins beyond the bind/wash/elute steps. Use a PCR cleanup kit immediately after PCR whenever the downstream application requires an inhibitor-free amplicon - primers and excess dNTPs left in the reaction will reduce sequencing quality, compete in ligation reactions, and inflate NGS background noise.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Oligo Clean &amp; Concentrator: Precisely designed systems for the quick retrieval of single-stranded DNA, RNA, and oligonucleotides from enzymatic processes.
</li>
<li dir="ltr" aria-level="1">
OneStep&trade; PCR Inhibitor Removal: Tailored columns and 96-well formats engineered to eliminate polyphenols, humic acids, and lipids that hinder PCR kinetics.
</li>
<li dir="ltr" aria-level="1">
ssDNA/RNA Clean &amp; Concentrator&trade;: An adaptable method for purifying and concentrating small nucleic acid segments, ensuring isoform specificity.
</li>
<li dir="ltr" aria-level="1">
ZR-96 High-Throughput Systems: Scalable formats of 96-well plates designed for automated lab processes, guaranteeing reproducibility-validated outcomes across extensive sample groups.
</li>
</ul>
&nbsp;
<h2>How to Choose a PCR Cleanup Kit</h2>
&nbsp;
Binding Capacity
Standard PCR cleanup columns bind up to 5-25 ug of DNA, depending on the kit; 25 ug capacity (Thermo Fisher GeneJET) accommodates typical preparative PCR reactions. For high-yield PCR reactions producing &gt;25 ug of amplicon, split the reaction across two columns and pool the eluates, or use a higher-capacity kit format.
Fragment Size Range
Standard PCR cleanup kits retain fragments &gt;=100 bp and discard everything shorter. For amplicons between 70 and 100 bp (e.g., short RT-qPCR products or miRNA amplicons), use a kit with an extended lower size limit -- the Thermo Fisher GeneJET kit retains fragments from 25 bp to 20 kb, covering this range. For amplicons shorter than 25 bp, conventional silica-column cleanup will not work; use gel purification or size-selective bead cleanup (e.g., AMPure XP at adjusted bead ratios).
Minimum Elution Volume
If the downstream application requires a high DNA concentration (Sanger sequencing needs &gt;20 ng/ul input), select a kit that elutes in as little as 20-25 ul without significant yield loss.&nbsp;
pH Indicator
Kits include a pH indicator dye in the binding buffer that turns yellow when the pH is correct for DNA binding and remains orange/red if the pH is too high (causing DNA loss). This is useful when purifying PCR products amplified in high-pH buffers. Kits without pH indicators require careful attention to binding buffer volume ratios to ensure efficient DNA adsorption.
96-Well Throughput
For screening workflows producing multiple amplicons per sample, use 96-well PCR purification plates with vacuum manifolds for simultaneous processing of all 96 wells. 96-well formats are essential for high-throughput genotyping and sequencing library preparation pipelines.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Key benchmarks for PCR cleanup kits: &gt;=70% DNA recovery for fragments 100 bp-10 kb, A260/A280 &gt;= 1.7 in eluate, complete removal of primers and dNTPs (confirmed by capillary electrophoresis with no peaks below 100 bp), and elution in &lt;=50 ul of water or TE. The Thermo Scientific GeneJET PCR Purification Kit specifies recovery rates up to 100% with a binding capacity of 25 ug per column in a 5-minute protocol. AAT Bioquest study comparing commercial PCR cleanup kit performance confirmed that silica-column kits consistently achieve primer and dimer removal equivalent to ethanol precipitation with significantly reduced protocol time. PCR cleanup is a mandatory step before NGS library preparation in nearly all sequencing-based workflows at institutions, including Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center, where primer and dimer contamination in libraries produces low-complexity sequencing reads that reduce effective coverage.
&nbsp;
Purify your amplicons and remove reaction noise&mdash;<a href="https://mbpinc.net/contact-us">reach out to MBP Inc.</a> now to obtain a quote for your PCR cleanup process.

PCR Cleanup Kits

MSDS - D7010-1

MSDS- 7010-2

MSDS - 7010-3

MSDS - D7010

ssDNA/RNA Clean & Concentrator™

Zymo Research ssDNA/RNA Clean &amp; Concentrator™: selective 17–200 nt ssDNA/ssRNA purification in ~10 min, ≥6 µl, 10 µg capacity, A260/280 &gt;1.8. D7010 (20 preps), D7011 (50 preps). Buffer components: Binding Buffer (D7010-1), Prep Buffer (D7010-2), Wash Buffer (D7010-3).

Equipment

Purity

Sample Source

Size Range

Yield

Manufacturer

ssDNA/RNA Clean & Concentrator™ (20 Preps.)

DNA/RNA Binding Buffer (10 ml)

DNA/RNA Binding Buffer (25 ml)

DNA/RNA Binding Buffer (50 ml)

DNA/RNA Prep Buffer (10 ml)

DNA/RNA Prep Buffer (200 ml)

DNA/RNA Prep Buffer (25 ml)

DNA/RNA Prep Buffer (50 ml)

DNA/RNA Wash Buffer (Concentrate) (12 ml)

DNA/RNA Wash Buffer (Concentrate) (24 ml)

DNA/RNA Wash Buffer (Concentrate) (6 ml)

ssDNA/RNA Clean & Concentrator™ (50 Preps.)

<h2 dir="ltr">What Are Lentiviral Control &amp; Reporter Constructs?</h2>
 Rigorous experimental design requires matched controls that enter the same delivery workflow as the test construct. Lentiviral control and reporter constructs use the same vector backbones, promoters, and packaging systems as experimental vectors &mdash; ensuring observed differences between treated and control cells reflect genuine biological effects rather than artefacts of the delivery process.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1"><a href="https://mbpinc.net/reagents/dna/cloning-services-products/lentivirus/over-expression/control/reporter/luciferase-reporter">Luciferase Reporters: </a>for reliable assay validation using selective promoter-specific vectors, fluorescent reporters, dual-reporter systems, and background control</li>
<li data-section-id="644n3l" data-start="108" data-end="253">Fluorescent Reporter Controls: GFP and RFP lentiviral vectors and viruses for monitoring transduction efficiency and transgene expression.</li>
<li data-section-id="19809zr" data-start="255" data-end="399">Blank Control Vectors &amp; Viruses: Empty lentiviral backbones for negative controls, promoter evaluation, and custom construct development.</li>
<li data-section-id="112pumm" data-start="401" data-end="569">Promoter &amp; UTR Reporter Systems:&nbsp; Reporter vectors designed for promoter activity and regulatory element analysis, including promoter and 5&prime; UTR reporter formats.</li>
<li data-section-id="1j5a9h3" data-start="571" data-end="736">Selectable Marker Controls: Control constructs with puromycin, neomycin, hygromycin, and blasticidin resistance markers for selection and workflow validation.</li>
</ul>
<h2 dir="ltr"> How to Choose Control &amp; Reporter Constructs</h2>
 Control Type Needed Empty vector controls carry only the selection marker (puromycin, blasticidin, or hygromycin resistance) without a gene of interest, providing the baseline reference for overexpression or knockdown experiments. Fluorescent Reporter Channel GFP and RFP lentiviral reporters allow real-time monitoring of transduction efficiency by flow cytometry or fluorescence microscopy, matching the experimental construct's promoter for an accurate expected-expression reference. Luciferase Reporter Pairing Firefly luciferase (P. pyralis) and Renilla luciferase (R. reniformis) are the two most commonly paired reporters; because they use different substrates (D-luciferin and coelenterazine, respectively), both signals can be measured sequentially from the same sample. Promoter-less Baseline A promoter-less dual luciferase control vector provides a no-promoter baseline against which promoter-driven reporter constructs are normalised &mdash; useful for establishing background signal levels. Vector Backbone Matching Using a matched vector backbone ensures observed differences between experimental and control cells are due to the transgene, not differences in vector, promoter strength, integration preference, or selection marker. 
<h2 dir="ltr"> Specifications Context</h2>
 Because lentiviral integration is heritable, transduced cells maintain stable reporter gene expression across cell divisions &mdash; stable luciferase or GFP reporter cell lines generated this way are widely used in drug screening, pathway activation assays, and long-term in vivo imaging. Through ABM, MBP can supply promoter-reporter lentiviral constructs covering human and mouse promoters, driving firefly luciferase or GFP expression, as plasmid DNA or pre-packaged particles. Both plasmid and pre-packaged formats are available for immediate dispatch, with institutional account pricing available for core facilities. Core facilities managing reporter constructs across multiple research groups often standardise on a small set of validated control vectors to simplify inventory and ensure consistency across projects.&nbsp; 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today to get high-quality lentiviral control/reporters for your lab.

Control/Reporter

Lentiviral Vector Amplification Protocol

AAV Infection Guideline

AAV Packaging Protocol

Selection-Drug Killing Curve

Lentivector Packaging Protocol

Lentivirus Infection Protocol

β-Gal Control

LV007 by Applied Biological Materials is a pre-packaged β-galactosidase (LacZ) lentiviral control virus supplied as 4 × 500 µL. Ready to transduce — no packaging required. Standard irrelevant-gene transduction control and positive reporter control for lentiviral experiments; detectable by X-gal staining or ONPG/CPRG enzyme assay. Research Use Only.