Brands

<h2 data-section-id="v1drft" data-start="0" data-end="43">What are cloning chemicals and reagents?</h2>
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Cloning chemicals and reagents cover the enzymes, buffers, and molecular biology-grade chemicals used in DNA fragment preparation, modification, and purification within a cloning workflow, including restriction endonucleases, T4 DNA ligase and other DNA-modifying enzymes (phosphatases, polymerases), reaction buffers, and plasmid extraction reagents. Related entities include restriction enzyme digestion, DNA dephosphorylation/phosphorylation, plasmid DNA purification (column-based and magnetic bead-based), and enzyme unit definitions.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Targeted XJa and XJb strains for faster recovery of proteins.</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Mix &amp; Go! solutions for optimized and dependable bacterial transformation.</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Culture Media &amp; Supplements used for yeast research, and include YPD Plus and mating pheromones.</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Extraction and Lysis Reagents include reliable genomic preparation and yeast protein kits.</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Chromogenic Substrates and Enzymatic Inducers with highly pure X-GAL and IPTG for cloning and blue-white screening.</li>
</ul>
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<h2 data-section-id="1daajgc" data-start="866" data-end="913">How to choose cloning chemicals and reagents</h2>
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Restriction enzyme selection and buffer compatibility
Restriction enzymes require specific reaction buffers, and many modern enzyme lines use a single universal buffer compatible across multiple enzymes to simplify double-digest reactions; confirm buffer compatibility when combining enzymes from different suppliers or using older single-buffer-per-enzyme formulations.
Enzyme unit definitions and reaction scale
Restriction enzyme and ligase activity is defined in units per microliter, with one unit typically defined as the amount needed to digest or ligate a defined quantity of standard substrate DNA under specified conditions in a set time; scale enzyme quantity to the amount of DNA being processed rather than assuming a fixed volume works for all reaction sizes.
Plasmid purification grade
Plasmid DNA extraction reagents range from molecular biology grade, suitable for general cloning and sequencing, to transfection-grade or endotoxin-free formulations required for applications such as lentiviral packaging or mammalian cell transfection, where endotoxin contamination can reduce transfection efficiency or cause cell stress.
Dephosphorylation for preventing vector self-ligation
Alkaline phosphatase treatment of a linearized vector before ligation removes 5' phosphate groups, preventing vector self-ligation and improving the ratio of recombinant clones; this step is particularly useful when the vector and insert share compatible sticky ends that could otherwise religate without insert incorporation.
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<h2 data-section-id="1fljvas" data-start="3459" data-end="3484">Specifications context</h2>
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Restriction enzymes and ligases are supplied at defined unit concentrations (commonly 10&ndash;20 units per microliter for restriction enzymes), with reaction buffers formulated for specific pH and salt conditions optimized for enzyme activity. Plasmid purification kits are differentiated by yield (micrograms of DNA recovered), purity (A260/A280 ratio), and endotoxin level (endotoxin units per microgram), with endotoxin-free kits specifying levels below a defined threshold suitable for transfection applications.&nbsp;
This sub-category is part of MBP's cloning catalog alongside ligation independent cloning kits and transformation kits and competent cells, used downstream of enzyme-based fragment preparation. Labs producing custom lentivirus for immortalization can also review MBP's cell immortalization catalog. MBP's specialist team can help confirm enzyme buffer compatibility and plasmid purification grade for a specific downstream application before order placement.
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today to request a quote for high-purity chemicals and reagents.

Chemicals/Reagents

MSDS

Mix & Go! 2X Stock Wash Buffer

Mix &amp; Go! 2X Stock Wash Buffer by Zymo Research — wash buffer component for the Mix &amp; Go E. coli transformation system. Room temperature storage. Available as T3001-2-10 (10 ml) and T3001-2-30 (30 ml).

Product Storage

Manufacturer

Shipping Condition

Storage Condition

Mix & Go! 2X Stock Wash Buffer (10 ml)

Mix & Go! 2X Stock Wash Buffer (30 ml)

<h2 data-section-id="k59sk3" data-start="0" data-end="52">What are transformation kits and competent cells?</h2>
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Transformation kits and competent cells consist of E. coli strains prepared to take up exogenous plasmid DNA, either through chemical competence (calcium chloride treatment, transformed via heat shock) or electrocompetence (transformed via electroporation), along with associated transformation buffers and protocols. Related entities include transformation efficiency (transformants per microgram), heat-shock and electroporation methods, strain genotype (recA, endA deficiency for plasmid stability), and downstream plasmid recovery for sequencing or transfection.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li>Mix &amp; Go! Transformation Kits, rapid protocols for preparation of competent E. coli in&nbsp;</li>
<li>ProClone&trade; Competent Cells with optimized E. coli strains for cloning and library generation.</li>
<li>Specialized Competent E. coli Strains ready-made JM109, DH5 Alpha, and Zymo 10B cells for regular and complex assembly.</li>
<li>Autolysis Competent Cells: designed for simplified cell lysis and streamlined protein expression using XJa and XJb strains</li>
<li>Frozen-EZ Yeast Transformation Kits for genetic manipulation of yeast strains.</li>
</ul>
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<h2 data-section-id="1ld88i5" data-start="812" data-end="876">How to choose transformation kits and competent cell products</h2>
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Chemically competent vs. electrocompetent cells
Chemically competent cells use a heat-shock protocol and are generally easier to handle for routine cloning, while electrocompetent cells require an electroporator but can achieve higher transformation efficiency for large plasmids, low-copy constructs, or DNA with modifications that reduce chemical transformation efficiency.
General cloning strains vs. protein expression strains
General cloning strains are selected for high transformation efficiency and genetic features (such as recA and endA deficiency) that improve plasmid stability and quality for downstream sequencing, while protein expression strains carry features supporting recombinant protein production, such as T7 RNA polymerase expression for IPTG-inducible systems; using a cloning strain for protein expression (or vice versa) generally gives suboptimal results.
Transformation efficiency requirements
Routine cloning of standard-sized plasmids works well with competent cells rated at 10^6&ndash;10^8 transformants per microgram, while ligation reactions with very low DNA concentrations (such as multi-fragment seamless assembly products) may benefit from higher-efficiency cells rated above 10^8 transformants per microgram.
Transformation kits may include a positive control plasmid and competent cells for validating transformation efficiency before using precious experimental DNA; this is particularly useful when troubleshooting low transformation yields to distinguish competent cell quality issues from ligation or assembly reaction issues.
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<h2 data-section-id="1fljvas" data-start="3947" data-end="3972">Specifications context</h2>
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Competent cell transformation efficiency is reported in transformants per microgram of standard control plasmid DNA, with general cloning strains commonly rated at 10^6&ndash;10^8 and high-efficiency formulations exceeding 10^8 transformants per microgram. Cells are typically supplied as frozen aliquots (e.g., 50&ndash;100 microliters per tube) for single-use transformations, shipped on dry ice and stored at -80&deg;C, with repeated freeze-thaw substantially reducing efficiency.&nbsp;
This sub-category is part of MBP's cloning catalog alongside ligation independent cloning kits and chemicals/reagents, the upstream steps of a cloning workflow. Labs producing custom lentivirus for immortalization can also review MBP's cell immortalization catalog. MBP's specialist team can help confirm strain genotype and transformation efficiency requirements for a specific cloning or expression project before order placement.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get high-quality transformation kits and competent cells for your lab.

Transformation Kits & Competetent Cells

DataSheet

Protocol

Mix & Go! Competent Cells - DH5 Alpha

Mix &amp; Go! Competent Cells - DH5 Alpha by Zymo Research — 108–109 transformants/µg pUC19, no heat shock, approximately 20-second transformation. Store at &lt;−70°C; integrity guaranteed 6 months. Available as T3007 (10×100 µl), T3009 (96×50 µl, 12×8-Tube Strips), and T3010 (96×50 µl with PCR plates and Cover Foils).

Additional Info

Genotype

Processing Time

Transformation Efficiency

Mix & Go! Competent Cells - DH5 Alpha (10 x 100 µl)

Mix & Go! Competent Cells - DH5 Alpha (96 x 50 µl)

Mix & Go! Competent Cells - DH5 Alpha (96 x 50 µl) w/ 96-well PCR plates and Cover Foils

Mix & Go! Competent Cells - HB101

Mix &amp; Go! Competent Cells - HB101 (T3011 / T3013) by Zymo Research are premade chemically competent E. coli HB101 cells with 10⁸–10⁹ transformants/µg efficiency and a 20-second protocol. Available in 10 × 100 µl (T3011) or 96 × 50 µl (T3013) formats; Dam⁺/Dcm⁺, recA⁻, max 10 kb plasmid, stored at −70°C to −80°C. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, and many more leading research institutes, serving labs across the United States, Canada, and internationally.

Mix & Go! Competent Cells - HB101 (10 x 100 µl)

Mix & Go! Competent Cells - HB101 (96 x 50 µl)

Mix & Go! Competent Cells - JM109

Mix &amp; Go! Competent Cells - JM109 by Zymo Research are chemically competent E. coli cells for plasmid transformation without heat shock. Transformation complete in 20 seconds; efficiency 10⁸–10⁹ transformants/µg (pUC19). Available as 10 × 100 µl tubes (T3003) or a 96 × 50 µl plate format (T3005); supports blue-white screening and plasmids up to 10 kb. Store at -70°C to -80°C.

Mix & Go! Competent Cells - JM109 (10 x 100 µl)

Mix & Go! Competent Cells - JM109 (96 x 50 µl)

Mix & Go! Competent Cells - TG1 (10 x 100 µl)

Mix &amp; Go! Competent Cells TG1 T3017 by Zymo Research are ready-to-use chemically competent E. coli cells (10 × 100 µL) with 10⁸–10⁹ transformants/µg, requiring no heat shock. TG1 strain supports blue-white screening (lacZ M15) and plasmids up to 10 kb. Storage at –70°C to –80°C.

Mix & Go! Competent Cells - XJa Autolysis (10 x 100 µl)

Mix &amp; Go! Competent Cells — XJa Autolysis (T3021) by Zymo Research. 10 × 100 µl. Transformation efficiency 108–109 cfu/µg. Built-in autolysis: 80–90% cell lysis in 10 min. No heat shock required.

Autolysis

DNA Extraction

DNA Stability

Protein Expression

Mix & Go! Competent Cells - XJa(DE3) Autolysis (10 x 100 µl)

T3031 Mix &amp; Go! Competent Cells — XJa(DE3) Autolysis by Zymo Research provides 10 × 100 µl aliquots of E. coli with 10⁸–9⁹ CFU/µg transformation efficiency and L-Arabinose-triggered λ endolysin autolysis for simplified protein expression and purification. Includes 1 ml 500× L-Arabinose; stored at -70°C to -80°C. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, and many more leading research institutes, serving labs across the United States, Canada, and internationally.

Mix & Go! Competent Cells - XJb Autolysis (10 x 100 µl)

T3041 by Zymo Research is the Mix &amp; Go! XJb Autolysis Competent Cells — an E. coli expression strain achieving 108–109 transformants/µg and 80–90% autolytic lysis within 10 minutes via L-Arabinose induction. Lon-/OmpT- for improved protein yields. Supplied as 10 × 100 µl with 1 ml 500× L-Arabinose. Store at -70°C to -80°C.

Cell Growth

Mix & Go! Competent Cells - XJb(DE3) Autolysis (10 x 100 µl)

Mix &amp; Go! Competent Cells - XJb(DE3) Autolysis (T3051) by Zymo Research — 10 × 100 µl. 108–109 transformants/µg; no heat shock. L-arabinose-induced autolysis: 80–90% cell lysis in ~10 min by freeze-thaw. DE3 lysogen for T7 expression. Lon-/OmpT- protease-deficient. Includes 500× L-arabinose. Storage −70°C to −80°C.

Mix & Go! Competent Cells - Zymo 10B

Mix &amp; Go! Competent Cells - Zymo 10B by Zymo Research — &gt;108 transformants/µg pUC19 without heat shock. Available as T3019 (10 × 100 µl) or T3020 (96 × 50 µl, 12 × 8-Tube Strips). Storage &lt;−70°C; 6-month integrity guarantee. Genotype: F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara leu) 7697 galU galK rpsL nupG λ-.

Mix & Go! Competent Cells - Zymo 10B (10 x 100 µl)

Mix & Go! Competent Cells - Zymo 10B (96 x 50 µl)

Mix & Go! Dilution Buffer

Mix & Go! Dilution Buffer by Zymo Research is used to dilute 2X concentrated buffers to 1X working concentration in the Mix & Go! E. coli Transformation System (T3001/T3002 kits). Available in 20 ml (T3001-4-20) and 60 ml (T3001-4-60) formats, stored at 0–8°C, it ensures optimal conditions for high-efficiency chemical transformation of E. coli.

Mix & Go! Dilution Buffer (20 ml)

Mix & Go! Dilution Buffer (60 ml)

Mix & Go! E. coli Transformation Kit (up to 20 ml)

Mix &amp; Go! E. coli Transformation Kit T3001 by Zymo Research enables preparation of up to 20 mL of competent cells with 10⁸–10⁹ transformants/µg pUC19 and no heat shock required. Compatible with DH5α, BL21, JM109, TG1, and XL10 Gold. Competent cell preparation completes in ~1–2 hours. Also available as a buffer-only set for up to 60 mL (T3002, ZymoBroth not included).