Name: Mix & Go! Competent Cells - XJb(DE3) Autolysis (10 x 100 µl)
SKU: T3051
Price: 249.85 USD
Availability: InStock

<h2 data-section-id="k59sk3" data-start="0" data-end="52">What are transformation kits and competent cells?</h2>
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Transformation kits and competent cells consist of E. coli strains prepared to take up exogenous plasmid DNA, either through chemical competence (calcium chloride treatment, transformed via heat shock) or electrocompetence (transformed via electroporation), along with associated transformation buffers and protocols. Related entities include transformation efficiency (transformants per microgram), heat-shock and electroporation methods, strain genotype (recA, endA deficiency for plasmid stability), and downstream plasmid recovery for sequencing or transfection.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li>Mix &amp; Go! Transformation Kits, rapid protocols for preparation of competent E. coli in&nbsp;</li>
<li>ProClone&trade; Competent Cells with optimized E. coli strains for cloning and library generation.</li>
<li>Specialized Competent E. coli Strains ready-made JM109, DH5 Alpha, and Zymo 10B cells for regular and complex assembly.</li>
<li>Autolysis Competent Cells: designed for simplified cell lysis and streamlined protein expression using XJa and XJb strains</li>
<li>Frozen-EZ Yeast Transformation Kits for genetic manipulation of yeast strains.</li>
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<h2 data-section-id="1ld88i5" data-start="812" data-end="876">How to choose transformation kits and competent cell products</h2>
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Chemically competent vs. electrocompetent cells
Chemically competent cells use a heat-shock protocol and are generally easier to handle for routine cloning, while electrocompetent cells require an electroporator but can achieve higher transformation efficiency for large plasmids, low-copy constructs, or DNA with modifications that reduce chemical transformation efficiency.
General cloning strains vs. protein expression strains
General cloning strains are selected for high transformation efficiency and genetic features (such as recA and endA deficiency) that improve plasmid stability and quality for downstream sequencing, while protein expression strains carry features supporting recombinant protein production, such as T7 RNA polymerase expression for IPTG-inducible systems; using a cloning strain for protein expression (or vice versa) generally gives suboptimal results.
Transformation efficiency requirements
Routine cloning of standard-sized plasmids works well with competent cells rated at 10^6&ndash;10^8 transformants per microgram, while ligation reactions with very low DNA concentrations (such as multi-fragment seamless assembly products) may benefit from higher-efficiency cells rated above 10^8 transformants per microgram.
Transformation kits may include a positive control plasmid and competent cells for validating transformation efficiency before using precious experimental DNA; this is particularly useful when troubleshooting low transformation yields to distinguish competent cell quality issues from ligation or assembly reaction issues.
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<h2 data-section-id="1fljvas" data-start="3947" data-end="3972">Specifications context</h2>
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Competent cell transformation efficiency is reported in transformants per microgram of standard control plasmid DNA, with general cloning strains commonly rated at 10^6&ndash;10^8 and high-efficiency formulations exceeding 10^8 transformants per microgram. Cells are typically supplied as frozen aliquots (e.g., 50&ndash;100 microliters per tube) for single-use transformations, shipped on dry ice and stored at -80&deg;C, with repeated freeze-thaw substantially reducing efficiency.&nbsp;
This sub-category is part of MBP's cloning catalog alongside ligation independent cloning kits and chemicals/reagents, the upstream steps of a cloning workflow. Labs producing custom lentivirus for immortalization can also review MBP's cell immortalization catalog. MBP's specialist team can help confirm strain genotype and transformation efficiency requirements for a specific cloning or expression project before order placement.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get high-quality transformation kits and competent cells for your lab.

Transformation Kits & Competetent Cells

MSDS

DataSheet

Protocol

Mix & Go! Competent Cells - XJb(DE3) Autolysis (10 x 100 µl)

Mix &amp; Go! Competent Cells - XJb(DE3) Autolysis (T3051) by Zymo Research — 10 × 100 µl. 108–109 transformants/µg; no heat shock. L-arabinose-induced autolysis: 80–90% cell lysis in ~10 min by freeze-thaw. DE3 lysogen for T7 expression. Lon-/OmpT- protease-deficient. Includes 500× L-arabinose. Storage −70°C to −80°C.

Mix & Go! Competent Cells - XJb(DE3) Autolysis (10 x 100 µl)

SKU : T3051