Brands

Orbital Shaker Accessories

<h2>What are orbital shaker accessories?</h2>
&nbsp;
Orbital shaker accessories are the platform attachments and holders that secure specific vessel types to a shaker platform during operation. Erlenmeyer flasks typically require spring-clip clamps that hold the flask securely while allowing unrestricted liquid movement, microplates use dedicated plate holders, tube rack inserts accommodate centrifuge and microcentrifuge tubes, and non-slip mats provide flexible placement for vessels during lower-speed mixing applications.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>
Dedicated Flask Platforms: Pre-configured platforms designed to securely hold specific sizes of Erlenmeyer flasks or standard microplates.
</li>
<li>
Universal Magnetic Platforms: Versatile, metallic base platforms engineered for instant, tool-free swapping of magnetic clamps and tube racks.
</li>
<li>
Magnetic Flask Clamps: Single-size and combo magnetic clamps designed for quick attachment to hold 25ml up to 500ml Erlenmeyer flasks.
</li>
<li>
Magnetic Tube Racks: Specially designed magnetic racks to accommodate culture tubes, microplates, and large conical bottles in various orientations.
</li>
</ul>
&nbsp;
<h2>How to choose orbital shaker accessories</h2>
&nbsp;
Select Erlenmeyer flask clamps by flask volume
Flask clamps are designed for specific flask sizes, including 125 mL, 250 mL, 500 mL, 1 L, and larger culture flasks. Each clamp is engineered to fit the dimensions of a particular flask size, ensuring secure retention during shaking.
Confirm compatibility with your shaker platform
Accessory mounting systems vary between manufacturers and shaker models. Before ordering, verify that the clamp, holder, or rack is listed as compatible with the specific orbital shaker platform being used.
Choose a microplate holder suitable for your application
Microplate holders secure standard microplates during incubation and mixing procedures. Different holder designs support different operating speeds and plate formats, making compatibility and intended use important selection criteria.
Use a non-slip platform mat for flexible vessel placement
Non-slip mats provide a simple solution for holding bottles, flasks, trays, and other containers at low to moderate shaking speeds. They are useful when vessel configurations change frequently and dedicated clamps are not required.
Verify tube rack capacity and platform fit
Tube rack inserts are available for common laboratory tube formats, including microcentrifuge tubes and conical centrifuge tubes. Confirm that the rack dimensions fit within the shaker platform and that the rack accommodates the desired number of samples per run.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Orbital shaker accessories are designed to keep vessels securely positioned while exposed to continuous circular motion. Flask clamps, plate holders, and tube racks should be selected according to vessel dimensions, operating speed, and platform compatibility. Stainless steel spring-clip clamps remain one of the most widely used accessory formats for laboratory culture applications due to their durability and secure retention.
<a href="https://mbpinc.net/contact-us">Conatct helpful team at MBP</a> for a quick customised quote.

MAGic Clamp™ tilted holder for 15mL and 50mL tube racks (max 3)

Benchmark Scientific MAGic Clamp tilted holder for 15 mL and 50 mL tube racks — accommodates up to 3 racks simultaneously. Magnetic tool-free attachment to MAGic Clamp (H1000-MR) compatible orbital shaker platforms. Promotes efficient tube mixing at a tilted angle.

Manufacture

MAGic Clamp™ universal platform (LG) for flasks & tube racks, 14 x 12"

The BT3000-MR MAGic Clamp™ Universal Platform (LG) by Benchmark Scientific is a 14 × 12 inch orbital shaker platform accessory that uses a magnetic clamping mechanism to secure Erlenmeyer flasks and tube racks during shaking on the BT3000 Orbi-Shaker — no clamp rings or bungee cords required. Designed for higher-capacity multi-flask runs.

MAGic Clamp magnetic clamp, 3000mL Erlenmeyer (max. 4)

MAGic Clamp magnetic clamp, 2000mL Erlenmeyer (max. 5)

MAGic Clamp Tube rack, 30x15mL & 20x50mL tubes (max. 1)

MAGic Clamp magnetic clamp, 1000mL Erlenmeyer (max. 4)

MAGic Clamp magnetic clamp, 500mL Erlenmeyer (max. 6)

MAGic Clamp magnetic clamp, 250mL Erlenmeyer (max. 8)

MAGic Clamp magnetic clamp, 125mL Erlenmeyer (max. 12)

MAGic Clamp magnetic clamp, 50mL Erlenmeyer (max. 20)

MAGic Clamp magnetic clamp, 25mL Erlenmeyer

MAGic Clamp magnetic clamp, one microplate (max. 4)

MAGic Clamp™ Magnetic Clamps Accessories

MAGic Clamp™ universal platform (SM) for flasks & tube racks (9.5 x 11.5")

H1000-MR by Benchmark Scientific is the 9.5×11.5 in MAGic Clamp™ standard universal platform for Orbi-Shaker™ and Incu-Shaker™ orbital shakers, serving as the magnetic base to which flask clamps (50 mL–3 L) and tube rack holders are magnetically attached. Platform only — clamps and rack accessories sold separately.

MAGic Clamp™ universal platform for flasks & tube racks (16 x16.5")

BT4010-MR by Benchmark Scientific is a 16 × 16.5-inch MAGic Clamp™ universal platform for the Orbi-Shaker™ XL and CO2 XL orbital shakers, using a patented magnetic attachment system that accepts flask clamps and tube racks tool-free. A single platform reconfigures instantly between Erlenmeyer flask formats (25 mL–2 L) and tube racks by swapping MAGic Clamp™ accessories (sold separately), eliminating format-specific platforms and lost fasteners in cell culture and fermentation workflows.

Weight

MAGic Clamp™ universal platform for flasks & tube racks (18 x18")

The H1010-MR by Benchmark Scientific is an 18 x 18 inch MAGic Clamp universal platform for Erlenmeyer flasks and tube racks on the Benchmark Incu-Shaker 10L and Orbi-Shaker XL. Magnetic vessel attachment — no mechanical clamps required. Available from MBP Inc.

<h2>What Are Cell-Free DNA Extraction Kits?</h2>
&nbsp;
A cell-free DNA (cfDNA) extraction kit purifies circulating cfDNA fragments from plasma, serum, or urine using silica spin-column or magnetic bead-based technologies. These kits are optimized to recover the short DNA fragments commonly found in liquid biopsy samples for downstream applications such as PCR, qPCR, next-generation sequencing (NGS), and methylation analysis. Choose a cfDNA-specific kit over a general genomic DNA kit whenever short circulating DNA fragments are the primary target.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>MagBio cfKapture ccfDNA Extraction Kit: for cell-free DNA isolation using advanced magnetic bead-based technology.</li>
<li>MAGicBead&trade; cfDNA Isolation Kit: for automated, high-throughput liquid biopsy workflows using high-binding paramagnetic particles.</li>
<li>Quick-cfDNA&trade; Serum &amp; Plasma Kit: for streamlined manual benchtop purification using rapid spin-column technology.</li>
</ul>
&nbsp;
<h2>How to Choose a Cell-Free DNA Extraction Kit</h2>
&nbsp;
Input Volume Range
Choose a kit compatible with your typical plasma, serum, or urine input volume. Larger sample volumes generally increase total cfDNA recovery but may require additional reagents and longer processing times.
Column-Based vs. Magnetic Bead-Based Chemistry
Silica spin-column kits are widely used for manual purification workflows, while magnetic bead-based kits simplify automation and high-throughput processing on compatible liquid handling platforms.
Elution Volume for Downstream Assays
Select an elution volume that matches the input requirements of your downstream applications, such as PCR, qPCR, or NGS library preparation, to minimize additional concentration or dilution steps.
Combined DNA/RNA Recovery
If your workflow requires analysis of both circulating DNA and RNA from the same specimen, consider kits designed to recover multiple nucleic acid types from a single sample.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Key considerations when selecting a cell-free DNA extraction kit include sample input volume, purification chemistry, compatibility with manual or automated workflows, and elution volume for downstream molecular assays. Magnetic bead-based systems are commonly selected for scalable, automated liquid biopsy workflows, while spin-column kits provide reliable manual purification for routine laboratory use. The optimal kit depends on your sample type, throughput requirements, and downstream analytical methods.
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Cell-Free DNA

Brochure

D4086-MSDS

D4086-4-MSDS

D4086-5-MSDS

D4086-Quick Protocol

D4086-Protocol

D4086-Automation Notes

MAGicBead™ cfDNA Isolation Kit

The MAGicBead™ cfDNA Isolation Kit by Zymo Research isolates NGS-ready cell-free DNA from up to 10 mL of plasma, saliva, urine, or other biofluids using magnetic bead technology, eluting in ≥15 µl with a 140–170 bp mono-nucleosomal peak. Compatible with KingFisher, Hamilton STAR, Tecan Fluent, and Opentrons OT-2. Available as D4086 (50 Preps. kit), D4086-4-70 (cfDNA Wash Buffer 70 mL), and D4086-5-2 (cfDNA Elution Buffer 2 mL).

Elution Volume

Fragment Sizes

Processing Time

Purity

Required Equipment

Sample Compatibility

Yield

Manufacturer

Shipping Condition

Storage Condition

MAGicBead™ cfDNA Isolation Kit (50 Preps.)

MAGicBead™ cfDNA Wash Buffer (70 ml)

MAGicBead™ cfDNA Elution Buffer (2 ml)

Serological Pipettes

<h2 dir="ltr"> What Are Serological Pipettes?</h2>
 Serological pipettes are calibrated, graduated, sterile polystyrene disposable pipettes used with a motorised pipette controller to transfer measured volumes of liquid in the 1&ndash;100 mL range. They are the primary tool for cell culture media transfer, buffer preparation, reagent dispensing in volumes above 1 mL, and serial dilutions requiring volumetric accuracy in the millilitre range. They differ from aspirating pipettes &mdash; which have no graduations and are used only for vacuum-assisted waste removal &mdash; and from microcentrifuge tubes, which are not dispensing instruments. Serological pipettes are the correct format for any measured liquid transfer &ge;1 mL in cell culture or reagent preparation; pair them with a motorised pipette controller for safe, ergonomic operation. 
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">1 mL to 50 mL Serological Pipettes &ndash; Standard range for routine and high-volume liquid transfer</li>
<li dir="ltr" role="presentation">Glass Serological Pipettes &ndash; Reusable options for solvent resistance and durability</li>
<li dir="ltr" role="presentation">Sterile Serological Pipettes &ndash; Gamma-sterilized for aseptic laboratory use</li>
<li dir="ltr" role="presentation">Non-Sterile Serological Pipettes &ndash; Cost-effective option for general applications</li>
<li dir="ltr" role="presentation">Graduated Serological Pipettes &ndash; For accurate volume measurement and dispensing </li>
</ul>
&nbsp;
<h2>How to Choose Serological Pipettes</h2>
 Volume: Standard Sizes and ISO 7550 Colour Coding ISO 7550 assigns a specific colour code to each serological pipette volume: 1 mL = red; 2 mL = yellow; 5 mL = blue; 10 mL = orange; 25 mL = green; 50 mL = purple (pink/violet depending on supplier). Colour coding allows quick visual volume identification when multiple sizes are in use simultaneously in a BSC. Packaging: Individual Wrap vs Bulk Individually wrapped sterile pipettes are required for cell culture, molecular biology, and aseptic technique. Plastic/plastic peel-open packaging eliminates paper particulate from paper/plastic wrappers &mdash; important in sensitive cell culture applications and for RNA work. Bulk-packed (canister or bag) formats are cost-effective for non-sterile applications such as buffer preparation and reagent dispensing in fume hoods. Construction: 1-Piece vs 3-Piece 1 mL, 2 mL, and 5 mL serological pipettes are typically one-piece moulded polystyrene constructions. 10 mL, 25 mL, and 50 mL pipettes use 3-piece ultrasonically welded construction (Globe Scientific) for the body-to-cone and body-to-cap joints &mdash; essential for the structural integrity of larger formats under aspiration vacuum. Tip and Graduation Features Negative graduations (markings above the nominal maximum) allow volumes slightly above nominal to be read. Fine-point tips with slow drip-off improve pipetting accuracy during cell culture media transfers. Some brands offer Wobble-not-style pipettes with reduced barrel flex for easier single-hand operation. Filter Plug Material Cotton filter plugs are standard; synthetic plugs (polyethylene or polypropylene) are preferred when processing organic solvents or any media that might wick through a cotton plug.
<h2 dir="ltr"> Compare Serological Pipette Volumes </h2>
&nbsp;
<ul>
<li>1 mL &ndash; Red ISO 7550 colour, 1-piece construction, 0.01 mL graduations; used for small-volume media transfer.</li>
<li>2 mL &ndash; Yellow ISO 7550 colour, 1-piece construction, 0.1 mL graduations; used for buffer preparation.</li>
<li>5 mL &ndash; Blue ISO 7550 colour, 1-piece construction, 0.1 mL graduations; used for media preparation and dilutions.</li>
<li>10 mL &ndash; Orange ISO 7550 colour, 3-piece construction, 0.1 mL graduations; used for flask media changes.</li>
<li>25 mL &ndash; Green ISO 7550 colour, 3-piece construction, 0.5 mL graduations; used for bulk media dispensing.</li>
<li>50 mL &ndash; Purple ISO 7550 colour, 3-piece construction, 1.0 mL graduations; used for large flask media changes.</li>
</ul>
<h2 dir="ltr"> Specifications Context</h2>
 Serological pipettes are manufactured from optically clear virgin polystyrene in Class 100,000 cleanroom conditions. Sterility: individually wrapped pipettes are EO-sterilised to SAL 10⁻⁶; non-pyrogenic, non-cytotoxic, DNase-free, and RNase-free per lot. ISO 7550 defines colour coding for serological pipettes; many US suppliers follow this standard, though it is not universally mandated. Calibrated to deliver (TD) with blow-out (marked with a double band or 'TD' marking) &mdash; all liquid must be expelled from the tip for accurate delivery. Globe Scientific, Nest Scientific, and Falcon remain the dominant serological pipette brands in North American cell culture labs.&nbsp; 
&nbsp;
Improve consistency and reduce handling variation with reliable serological pipette solutions. Request a quote from <a href="https://mbpinc.net/contact-us">the expert team at Molecular Biology Products Inc. (MBP).</a>

MBP Brand - Serological Pipettes

MBP Brand Serological Pipettes are available in the following variants: SP005, SP0010, SP0025, SP0050.SP000 by MBP: MBP Brand sterile serological pipettes — individually wrapped, EO-sterilized SAL 10⁻⁶, non-pyrogenic, DNase/RNase-free polystyrene. Available in 5 mL (200/pack), 10 mL (200/pack), 25 mL (100/pack), and 50 mL (75/pack).

5ml Serological Pipets, MBP, 200/Pk, 1200/Case

10ml Serological Pipets, MBP, 200/Pk, 1200/Cs

25ML Serological Pipets, MBP, 100/Pk, 600/Cs

50ML Serological Pipets, MBP, 75/Pk, 450/Case

Microcentrifuge

<h2>What Are Microcentrifuges?</h2>
&nbsp;
Microcentrifuges are compact, high-speed benchtop centrifuges designed specifically for 0.2&ndash;2.0 mL microtubes and PCR strips, operating at 10,000&ndash;21,000 &times;g in a fixed-angle rotor. They are the primary centrifugation instrument in molecular biology, biochemistry, and clinical molecular diagnostics labs: nucleic acid pellet formation after ethanol precipitation, spin-column DNA/RNA extraction and PCR cleanup steps (typically specified at 10,000&ndash;16,000 &times;g for 30&ndash;60 seconds), cell debris clearance from lysates, protein precipitation supernatant recovery, and brief sample clarification before downstream analysis. Standard benchtop microcentrifuges reach 14,000&ndash;15,000 RPM (16,000&ndash;21,000 &times;g), while high-speed models reach up to 21,000 RPM and approximately 31,000 &times;g. Refrigerated variants maintain 0&ndash;4&deg;C for temperature-sensitive workflows.
&nbsp;
<h2>What You Will Find:</h2>
&nbsp;
<ul>
<li>High-Speed Microcentrifuges: For faster sample processing</li>
<li>Refrigerated Microcentrifuges: For temperature-sensitive handling</li>
<li>Benchtop Microcentrifuge Systems: For routine lab working</li>
<li>5D Digital Microcentrifuge: With customizable time and speed options</li>
<li>Mini Microcentrifuges: Compact units for rapid spin-down tasks</li>
<li>MC-24 High-Speed Microcentrifuge: Features touch screen control and high speed</li>
<li>Microplate Centrifuges: Developed for PCR plates and strip formats</li>
<li>Microcentrifuge Accessories: For expanded tube flexibility and compatibility</li>
</ul>
&nbsp;
<h2>How to Choose a Microcentrifuge</h2>
&nbsp;
Maximum RCF vs. Protocol Requirements
Most spin-column kit protocols specify 10,000&ndash;16,000 &times;g. A standard microcentrifuge reaching 14,000&ndash;16,000 &times;g covers these requirements. If your lab performs alcohol-precipitation DNA/RNA pelleting (12,000&ndash;16,000 &times;g) or virus concentration steps (20,000+ &times;g), choose a model reaching at least 16,000 &times;g. High-speed models reaching 21,000&ndash;31,000 &times;g are available for specialized applications.
Rotor Tube Positions and Capacity
Most microcentrifuge rotors hold 12&ndash;24 &times; 1.5/2.0 mL tubes per run. Laboratories processing larger batches benefit from 24-position rotors, while smaller research groups may find 12-position models sufficient. Confirm that adapters for 0.5 mL and 0.2 mL tubes are available if required by your workflows.
Refrigerated vs. Ambient Temperature
Refrigerated microcentrifuges are recommended for RNA extraction, protein stability studies, cell viability applications, and any protocol requiring 4&deg;C centrifugation. Standard room-temperature models are suitable for routine DNA extraction and PCR cleanup procedures.
Noise Level and Footprint
Modern brushless DC motor microcentrifuges typically operate at &le;52 dB, making them quieter than older brush-motor systems. Most units occupy 200&ndash;300 mm of bench width, so confirm that sufficient clearance is available when the lid is fully open.
Safety Features
Essential safety features include lid-lock mechanisms, imbalance detection, and over-speed protection. Laboratories handling infectious materials should verify that aerosol-tight sealed rotor lids are available for the selected model.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The standard spin-column step in most commercial DNA/RNA extraction kits specifies 10,000&ndash;16,000 &times;g for 30&ndash;60 seconds. Always confirm the rotor's maximum RCF from manufacturer specifications, since RPM values alone do not account for differences in rotor radius.
Microcentrifuge rotors are typically fixed-angle designs positioned at approximately 45&deg;, providing efficient pelleting and short sedimentation paths. Aerosol-tight sealed lids allow safe handling of BSL-2 samples and other applications requiring biocontainment. Modern brushless DC motors offer quieter performance, improved speed accuracy, and longer service intervals than legacy brush-motor systems.
Your search for high-performance microcentrifuges is over. <a href="https://mbpinc.net/contact-us">Contact now</a> with the expert team at Molecular Biology Products Inc. and get the right microcentrifuge for your lab today.

Product Literature

MC-12 Microcentrifuge with 12 place rotor Accessories

MC-12™HighSpeed Microcentrifuge (12 place)

The MC-12™ High Speed Microcentrifuge by Benchmark Scientific is a 12-place benchtop centrifuge reaching 15,000 rpm / 16,100 x g with a brushless, maintenance-free drive, aircraft-grade aluminum rotor, and a footprint under 9 inches wide. Standard configuration spins 12 x 1.5/2.0 ml microtubes; optional adapters extend to 0.5 ml and 0.2 ml tubes. Available in 115V (C1612) and 230V (C1612-E).

Speed

Capacity

Acceleration

Deceleration

Speed increment

Timer

Momentary

Dimensions

Electrical

Warranty

MC-12™ High Speed Microcentrifuge with 12 place rotor, 115v

MC-12™ High Speed Microcentrifuge with 12 place rotor, 230v

MC-24R™ Refrigerated High Speed Microcentrifuge w/ COMBI-Rotor Accessories

MC-24R™ Refrigerated High Speed Microcentrifuge (24 place)

The Benchmark MC-24R Refrigerated High Speed Microcentrifuge Series by Benchmark Scientific reaches 13,500 RPM (16,800 × g) with integrated refrigeration from –20 °C to +40 °C. The included COMBI-Rotor accepts 24 × 1.5/2.0 mL microtubes plus 2 PCR strips simultaneously. With 99 stored programs, adjustable accel/decel, and touchscreen control, it is built for nucleic acid purification, protein pelleting, and temperature-sensitive spin-column protocols. Available as C2417-R (COMBI-Rotor, 120V), C2417-R-E (COMBI-Rotor, 230V), C2417-R-NR (no rotor, 120V), C2417-R-NR-E (no rotor, 230V).

Temperature

MC-24R Refrigerated High Speed Microcentrifuge with COMBI-Rotor, 120V

MC-24R Refrigerated High Speed Microcentrifuge with COMBI-Rotor, 230V

MC-24R Refrigerated High Speed Microcentrifuge without rotor, 120V

MC-24R Refrigerated High Speed Microcentrifuge without rotor, 230V

Optional 8 x 5ml rotor for MC-24™ Touch

2ml Cryovial adapters for C2417-R5 rotor, 8/pk

MC-24™ Touch High Speed Microcentrifuge with COMBI-Rotor Accessories

MC-24™HighSpeed Microcentrifuge (24 place)

The MC-24™ HighSpeed Microcentrifuge by Benchmark Scientific is a 24-place benchtop unit reaching 13,500 RPM / 16,800 × g with a touch-screen interface — the first in its class. The COMBI-Rotor™ holds both 1.5/2.0 ml microtubes and 0.2 ml PCR tubes or strips without adapters, supporting molecular biology, genomics, and clinical sample-processing workflows. Available in 115V (C2417) and 230V (C2417-E).

MC-24™ Touch High Speed Microcentrifuge with COMBI-Rotor, 115v

MC-24™ Touch High Speed Microcentrifuge with COMBI-Rotor, 230v

<h2 data-section-id="185nm5c" data-start="0" data-end="38">What is classic cell culture media?</h2>
&nbsp;
Classic cell culture media refers to the foundational basal medium formulations that have underpinned mammalian cell culture for decades, including DMEM (Dulbecco's Modified Eagle Medium), MEM (Minimum Essential Medium), RPMI 1640, Ham's F-10, Ham's F-12, IMDM (Iscove's Modified Dulbecco's Medium), and DMEM/F-12. These media provide the amino acids, vitamins, glucose, salts, buffering agents, and other nutrients required to support cell survival and growth in vitro. They form the basis of many modern specialized, serum-free, and advanced media formulations used in academic research, biotechnology, pharmaceutical development, and biomanufacturing.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
&nbsp;Availability of both low and high carbohydrate levels of balanced basal formulations for the distinct metabolic requirements of diverse mammalian cell lines.
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Maintenance of precise physiological pH during long-term incubation cycles using optimized buffering configurations with integrated chemical stabilizers&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Nutrient-dense cell media supplemented with vitamins and essential amino acids to support&nbsp; hybridomas and rapidly proliferating continuous cultures
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Optimized serum reduction along with enhanced cell attachment using&nbsp;Multi-purpose 1:1 formulation hybrids&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Salt solutions supplemented with essential minerals to facilitate enzyme preparation, cell washing, and reliable short-term osmotic maintenance.
</li>
</ul>
&nbsp;
<h2 data-section-id="1blio5n" data-start="931" data-end="964">How to choose a classic medium</h2>
&nbsp;
DMEM vs MEM
DMEM contains up to four times the amino acids and vitamins found in MEM, along with higher glucose concentrations, making it suitable for cell types with elevated nutrient demands. It is widely used for primary cells, stem cells, and many transformed mammalian cell lines. MEM, based on the original Eagle's formulation, remains a common choice for standard monolayer culture and is available with different salt formulations to support various applications.
RPMI 1640 for suspension and hematopoietic culture
RPMI 1640 was originally developed for culturing human leukemic cells and remains the preferred medium for lymphocytes, hybridomas, and other hematopoietic cell types. Its formulation includes glutathione and elevated levels of biotin, vitamin B12, and PABA. Although designed for suspension cultures, RPMI 1640 also supports a wide range of adherent cell lines when appropriately supplemented.
Ham's F-10/F-12 for CHO and specialized applications
Ham's nutrient mixtures were developed for CHO cells and other specialized cell types. Their formulations include additional nutrients such as zinc, putrescine, hypoxanthine, and thymidine, making them useful for hepatocyte culture, toxicity testing, viral fusion studies, and other specialized applications. These media can be used with or without serum depending on the experimental requirements.
DMEM/F-12 for complex, rich-medium applications
DMEM/F-12 combines the nutrient-rich composition of DMEM with the specialized components found in Ham's F-12. The resulting formulation supports demanding cell types including MDCK cells, glial cells, fibroblasts, and endothelial cells. It is also widely used as the foundation for many serum-free and advanced culture systems.
IMDM for high-density rapid proliferation
IMDM is an enriched derivative of DMEM that contains additional amino acids, vitamins, HEPES buffer, sodium pyruvate, and potassium nitrate. Its enhanced nutrient profile makes it particularly suitable for rapidly proliferating and high-density cultures such as Jurkat cells, COS-7 cells, and macrophages.
Liquid vs powder format
Liquid classic media are supplied ready for use after the addition of serum and required supplements. Powdered media offer lower shipping costs and greater flexibility for high-volume laboratories but require reconstitution, sodium bicarbonate addition, and sterile filtration before use.
&nbsp;
<h2 data-section-id="1fljvas" data-start="4382" data-end="4407">Specifications context</h2>
&nbsp;
Classic media are manufactured according to their original published formulations, with only stability-related modifications where necessary. Most liquid formulations contain sodium bicarbonate as the primary buffering system and require incubation in a 5&ndash;10% CO₂ atmosphere to maintain physiological pH. Powdered formulations require reconstitution, sodium bicarbonate supplementation, and sterile filtration before use.
As of 2026, DMEM, DMEM/F-12, and RPMI 1640 remain the most widely used classic cell culture media across academic, biotechnology, pharmaceutical, and biomanufacturing laboratories. IMDM continues to gain popularity for high-density culture and bioprocessing applications.
For recombinant growth factors and cytokines used alongside classic media, see Recombinant Proteins. For supplementation reagents such as GlutaMAX, NEAA, sodium pyruvate, and HEPES, see Cell Culture Supplements &amp; Additives. Browse Mammalian Cell Culture for the broader category.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get optimized classic media for your lab.

Classic Media

MEM α, No Nucleosides Medium

MEM α, No Nucleosides Medium (TM512) by Applied Biological Materials is a 500 ml sterile basal medium with Earle's salts, L-glutamine, sodium bicarbonate (2.2 g/L), and phenol red — without ribonucleosides or deoxyribonucleosides — for DHFR-negative cell selection and general mammalian cell culture. Requires 5–10% CO₂. Store at 2–8°C, protected from light.

Unit Quantity

Sterility

Appearance

Disclaimer

Caution

Vacuum Pump

<h2>What are laboratory vacuum pumps?</h2>
&nbsp;
Laboratory vacuum pumps are instruments that remove gas from a connected vessel or system to create sub-atmospheric pressure for applications including rotary evaporation of solvents, vacuum filtration through B&uuml;chner funnels and membranes, SpeedVac centrifugal concentration, gel drying, lyophilization rough vacuum, and degassing of solvents and solutions. MBP carries vacuum pumps from Vacuubrand, Welch, and ULVAC, which together cover the full range of laboratory vacuum needs from moderate filtration vacuum to deep vacuum for turbomolecular backing, available in oil-free diaphragm and oil-sealed rotary vane configurations.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul style="list-style-type: disc;">
<li dir="ltr"><a href="https://mbpinc.net/equipments/sample-processing/pumps/vaccum-pumps/edwards-pumps" style="color: #236fa1;">Edwards Pumps: </a>Reliability at its best &ndash; with strong vacuum pumps that are made to work for high-performing industrial and research purposes.</li>
<li dir="ltr"><a href="https://mbpinc.net/equipments/sample-processing/pumps/vaccum-pumps/agilent-pumps" style="color: #236fa1;">Agilent Pumps: </a>Get the most out of high vacuum pumps engineered for mass spectrometry, leak testing, and other advanced lab methods.</li>
<li dir="ltr"><a href="https://mbpinc.net/equipments/sample-processing/pumps/vaccum-pumps/vacuubrand-pumps">Vacuubrand Pumps: </a>Take advantage of German-engineered diaphragm pumps and vacuum control devices that are resistant to chemical agents and easy to maintain.</li>
<li dir="ltr"><a href="https://mbpinc.net/equipments/sample-processing/pumps/vaccum-pumps/welch-pumps">Welch Pumps: </a>Perfect for all your routine lab procedures using vacuum pumps made with quality materials for durability and versatility.</li>
<li dir="ltr"><a href="https://mbpinc.net/equipments/sample-processing/pumps/vaccum-pumps/ulvac-pumps" style="color: #236fa1;">ULVAC Pumps:</a> Use proven Japanese vacuum technology renowned for reliable and effective operations in manufacturing and thin-film production.</li>
<li dir="ltr"><a href="https://mbpinc.net/equipments/sample-processing/pumps/vaccum-pumps/vaccum-pump-accessories" style="color: #236fa1;">Vacuum Pump Accessories:</a> Boost your system&rsquo;s capabilities with oil mist filters, traps, and other accessories to optimize pump performance.</li>
</ul>
&nbsp;
<h2>How to choose between vacuum pump brands and types</h2>
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Choose Vacuubrand for premium PTFE diaphragm and chemistry vacuum systems
Vacuubrand diaphragm pumps are known for their high-quality PTFE-wetted construction, precise chemistry vacuum control systems with automatic set-point regulation, and a broad range of configurations from single-stage to two-stage models, making them the preferred brand for demanding rotary evaporation and concentration applications where reproducible vacuum control is important.
Choose Welch for reliable, cost-effective diaphragm and corrosion-resistant vacuum
Welch (by Gardner Denver) diaphragm pumps offer solid performance for routine filtration and moderate vacuum applications at a competitive price point, with the Dryfast ULTRA series as their flagship oil-free, chemically resistant product line covering most common laboratory vacuum needs.
Choose ULVAC for applications requiring deeper vacuum
ULVAC rotary vane oil-sealed pumps achieve deeper ultimate vacuum levels than oil-free diaphragm pumps, making them appropriate for applications like thin-film deposition, high-vacuum instruments, manifold vacuum systems, or backing turbomolecular pumps, where vacuum levels below 1 mbar are required.
Check the accessories catalog for vacuum tubing, traps, and gauges
Accessories include cold traps and chemical traps to protect pumps from solvent and corrosive vapors, vacuum tubing (rubber, silicone, or PTFE), and vacuum gauges and controllers for monitoring and regulating applied vacuum.
Match ultimate vacuum and pumping speed to your application requirements
Ultimate vacuum determines the lowest pressure achievable, while pumping speed determines how quickly the system reaches and maintains that vacuum. Both must be matched to system volume and gas load from evaporation, filtration, or outgassing.
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<h2>Specifications context</h2>
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The choice between oil-free diaphragm and oil-sealed rotary vane pumps is primarily determined by required ultimate vacuum and chemical exposure. Diaphragm pumps provide oil-free, low-maintenance vacuum suitable for most chemistry and biology applications above ~1&ndash;5 mbar, while rotary vane pumps achieve deeper vacuum but require oil changes and carry contamination risk.
As of 2026, oil-free diaphragm pumps from Vacuubrand and Welch cover most standard molecular biology and chemistry vacuum applications, while ULVAC rotary vane and dry pumps serve specialized deep-vacuum requirements.
Do you need any assistance figuring out your displacement or pressure requirements? Our experts at MBP are here to help. <a href="https://mbpinc.net/contact-us">Contact us for a customized quote.</a>

Vaccum Pump

MF30 Exhaust Oil Mist Filter for Edwards E2M28 Vacuum Pumps

The MF30 by Edwards Vacuum (A462-33-000) is an exhaust oil mist filter for the Edwards E2M28 rotary vane vacuum pump, delivering greater than 99.9% filtration efficiency (DOP test) at a rated flow of 30 m³/h via NW25 connections. The kit includes NW25 elbow, clamp, ¾-inch BSP adapter, and Co-Seal for direct installation on the pump exhaust port.