Brands

<h2 dir="ltr">What Are Specialized Cloning Vectors &amp; Viral Tools?</h2>
 Standard lentiviral and retroviral expression vectors meet the needs of most overexpression and knockdown workflows, but many cell biology and translational research questions demand purpose-engineered constructs with specialised functions &mdash; subcellular localisation, multi-gene co-expression, or conditional activation. Select the format best suited to your experimental requirements.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
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<a href="https://mbpinc.net/reagents/dna/cloning-services-products/specialized-cloning-vectors/-virus/organelle-labeling-viruses">Organelle Labeling Viruses: </a>for live-cell visualization and functional pathway analysis of cellular structures in mammalian systems
</li>
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Targeted fluorescent labeling of specific cellular structures using viral delivery.
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Stable expression of organelle-specific markers for long-term live-cell imaging.
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Compatible with fluorescence microscopy for dynamic cellular imaging applications.
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<li data-section-id="8k7pm2" data-start="506" data-end="603" data-is-last-node="">Suitable for studying organelle organization, transport, and cellular function in living cells.</li>
</ul>
<h2 dir="ltr"> How to Choose Specialized Cloning Vectors</h2>
 Subcellular Targeting Needs Organelle-targeted fluorescent labeling lentiviral particles deliver fluorescent protein constructs targeting mitochondria, endoplasmic reticulum, nucleus, Golgi, lysosome, peroxisome, plasma membrane, autophagosome, and cytoskeleton &mdash; providing a stable alternative to chemical dyes for live-cell imaging. Multi-Gene Co-Expression Bicistronic vectors using IRES or 2A self-cleaving peptide sequences enable co-expression of two genes (e.g., a gene of interest and a fluorescent marker) from a single transcript or vector. Conditional Expression Requirements Conditional DIO/FLEX vectors restrict transgene expression to Cre-expressing cells, essential for circuit-specific neuroscience research and in vivo conditional gene expression studies. Temporal Control Needs Inducible constructs (Tet-On/Tet-Off systems) provide doxycycline-regulated transgene activation for experiments requiring temporal control over expression timing. Delivery Format Most specialised constructs are available as lentiviral particles for stable, long-term expression in both dividing and non-dividing cell types, including primary cells and in vivo models. 
<h2 dir="ltr"> Specifications Context</h2>
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Traditional subcellular organelle imaging relies on chemical dyes (e.g., MitoTracker, LysoTracker, ER-Tracker) or antibody-based immunofluorescence in fixed cells; however, chemical dyes are transient and may exhibit cytotoxicity at working concentrations, while antibody-based methods are limited to endpoint imaging. Viral organelle-targeted fluorescent expression systems enable stable, genetically encoded labeling for long-term live-cell imaging and dynamic subcellular tracking. These constructs are supplied in plasmid DNA and pre-packaged viral formats and support applications including organelle dynamics, trafficking studies, and multicolor imaging workflows. Specialized cloning vectors and viral tools are sourced from established manufacturers, including Applied Biological Materials (ABM), and product availability reflects MBP&rsquo;s catalogue as of mid-2026.
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<a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact the MBP team </a>today and get specialized cloning vectors for your lab.

Specialized Cloning Vectors/ Viruses

Specialized Cloning Vectors/ Virus

LV010156 Vector Sequence

ERT2-Cre-ERT2 Lentivirus (Bacteriophage P1) (pLenti-III)

Unit Quantity

Vector

Titer

Storage Condition

Shipping Condition

<h2>What are bisulfite conversion kits?</h2>
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Bisulfite conversion kits are all-in-one systems that bundle every reagent and column needed for the sodium bisulfite conversion workflow into a single, pre-optimized format, covering the denaturation and sulfonation steps, desulfonation with an alkali reagent, and a post-conversion cleanup step that removes the bisulfite and other reaction byproducts before the converted DNA is used in downstream analysis. This contrasts with standalone Bisulfite Conversion Reagents &amp; Buffers, which lets labs assemble their own optimized protocol from individual components.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Standard bisulfite conversion kits for routine DNA methylation analysis&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Combined transcriptomic and epigenetic studies with RNA and DNA methylation conversion kits</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">High-throughput 96-well bisulfite conversion kits for wider-scale screening applications</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Automation-compatible bisulfite kits for simplified workflow integration</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Magnetic bead-based bisulfite conversion kits for enhanced purification and recovery efficiency</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Direct bisulfite conversion kits for streamlined sample processing&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Rapid conversion kits for methylation analysis workflows that are time-sensitive&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Starter kits for validation and optimized setup of bisulfite conversion protocols</li>
</ul>
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<h2>How to choose a bisulfite conversion kit</h2>
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Match kit format to your downstream sequencing or PCR application
Kit versions optimized for WGBS work at larger input scales with library prep in mind, while RRBS-compatible kits often include restriction enzyme digestion components to enrich CpG-dense regions before conversion, and MSP-focused kits may include primers or protocol guidance for post-conversion methylation-specific PCR.
Check the kit's validated input DNA range against your sample type
Different bisulfite conversion kits are validated for different input DNA ranges, and kits validated for low-input or cell-free DNA samples specifically address the additional fragmentation and yield challenges that these sample types face during the harsh chemical conversion step.
Confirm the kit includes an unmethylated conversion control or specify one separately
A quality bisulfite conversion kit should provide, or clearly specify, an unmethylated control to run alongside samples, since tracking conversion efficiency across the kit's components helps catch kit-specific variability in conversion completeness between different lots or runs.
Consider kits with optimized FFPE protocols when working with formalin-fixed tissue
Formalin-fixed, paraffin-embedded tissue DNA is already fragmented before conversion; kits with FFPE-specific protocol variants include modifications like shortened incubation times and carrier reagents to minimize the further fragmentation this already-compromised input type would suffer under standard conversion conditions.
Evaluate throughput format options to match your sample volume
Some bisulfite conversion kits are available in spin column formats suited to individual sample processing, while plate-based or automation-compatible formats support higher-throughput sample sets, making format an important practical consideration beyond kit chemistry alone.
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<h2>Specifications context</h2>
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Bisulfite sequencing is widely regarded as the gold standard for DNA methylation analysis at single-base resolution, and a key advantage of using a complete kit over standalone reagents is that the included cleanup step reliably removes residual bisulfite and salt from the converted DNA, which would otherwise inhibit downstream PCR amplification and library preparation. As of 2026, enzymatic conversion kits (covered in Non-Bisulfite Kits &amp; Reagents) are increasingly positioned as an alternative that avoids the DNA damage introduced by chemical bisulfite treatment, though bisulfite conversion kits remain broadly used and well-validated across the largest range of downstream applications.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP and get high-quality bisulfite conversion kits for your lab.

Bisulfite Conversion Kits

Data Sheet

Protocol

EZ DNA Methylation-Direct™ Kit

The EZ DNA Methylation-Direct™ Kit (D5020) by Zymo Research performs &gt;99.5% bisulfite conversion directly from blood, tissue, cells, FFPE, and LCM samples — no prior DNA purification required. Accepts 50 pg–2 µg DNA or 10–10⁵ cells; elutes in ≥10 µl. Compatible with PCR, sequencing, microarrays, and NGS. 50 reactions (D5020) or 200 reactions (D5021).

Applications

Conversion

Elution Volume

Equipment

Input

Processing Time

Recovery

Sample Source

Manufacturer

EZ DNA Methylation-Direct™ Kit (50 Rxns.)

EZ DNA Methylation-Direct™ Kit (200 Rxns.)

D5005-MSDS

D5005-Protocol

EZ DNA Methylation-Gold™ Kit

D5005 by Zymo Research converts unmethylated cytosines to uracil with &gt;99% efficiency in under 3 hours at 64°C, using 500 pg–2 µg of input DNA. The EZ DNA Methylation-Gold™ Kit (50 rxns) supports bisulfite sequencing, MSP, pyrosequencing, Illumina EPIC/450K arrays, and endonuclease-based methylation analysis. All reagents store at room temperature; converted DNA is stable at −20°C.

EZ DNA Methylation-Gold™ Kit (50 Rxns.)

EZ DNA Methylation-Gold™ Kit (200 Rxns.)

DataSheet

MSDS

Recommendations

EZ DNA Methylation-Lightning Automation Kit (1 x 96 prep)

The D5049 by Zymo Research is a 96-prep EZ DNA Methylation-Lightning Automation Kit combining &gt;99.5% bisulfite conversion efficiency (98°C 8 min + 54°C 60 min) with automated magnetic bead purification compatible with Hamilton STAR/STARlet, Tecan Fluent, and KingFisher Flex robotics. Input range 100 pg–2 µg; &gt;80% recovery; A260/A280 ≥ 1.8; optimized for Illumina Infinium arrays, WGBS, and MSP workflows.

Binding Capacity

Conversion Efficiency

DNA Purity

Input Recovery

Required Equipment

User Provided Materials

Data Sheet (Fragmented DNA)

Data Sheet (NGS)

D5030/D5031-MSDS

D5030E/D5031E-MSDS

D5030/D5031-Protocol

D5030E/D5031E-Protocol

EZ DNA Methylation-Lightning Kit

D5030 EZ DNA Methylation-Lightning™ Kit by Zymo Research delivers >99.5% bisulfite conversion efficiency with >80% DNA recovery from 100 pg–2 µg input in under 1.5 hours, 50 reactions, all components stable at room temperature.

EZ DNA Methylation-Lightning™ Kit (50 Rxns.)

EZ DNA Methylation-Lightning™ Kit (50 Rxns.) (CE-IVD)

EZ DNA Methylation-Lightning™ Kit (10 Rxns.)

EZ DNA Methylation-Lightning™ Kit (200 Rxns.)

EZ DNA Methylation-Lightning™ Kit (200 Rxns.) CE-IVD

EZ DNA Methylation-Startup™ Kit (50 Rxns.)

D5024 by Zymo Research is the EZ DNA Methylation-Startup™ Kit (50 rxns) — an all-inclusive bisulfite conversion kit including EZ DNA Methylation-Direct Kit, Universal Methylated Human DNA Standard with control primers, and ZymoTaq DNA Polymerase PreMix. Conversion efficiency >99.5%; input 50 pg–2 µg; recovery >80%; 4-hour processing.

Applicable For

D5001-MSDS

D5001-Protocol

D5001-App Note

EZ DNA Methylation™ Kit

D5001 by Zymo Research is the EZ DNA Methylation Kit — a 50-reaction bisulfite conversion system delivering &gt;99% cytosine conversion from 500 pg to 2 µg input DNA, with &gt;80% recovery and ≥10 µl elution volume. Validated for Illumina Infinium MethylationEPIC BeadChip, NGS bisulfite sequencing, MSP, and pyrosequencing. Requires a thermocycler with heated lid; total processing time 12–16 hours. Made in USA by Zymo Research.

EZ DNA Methylation™ Kit (50 Rxns.)

EZ DNA Methylation™ Kit (200 Rxns.)

<h2>What are ChIP kits?</h2>
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ChIP (chromatin immunoprecipitation) kits provide all the components needed to enrich a specific protein-DNA complex from fixed, sheared chromatin using an antibody against the protein of interest, whether a histone modification like H3K4me3 or a DNA-binding protein like a transcription factor. A complete ChIP kit covers: formaldehyde crosslinking of proteins to DNA in intact cells or tissue, cell lysis and nuclear extraction, chromatin shearing to appropriate fragment sizes, immunoprecipitation using an antibody pre-bound to protein A/G beads, sequential washing to remove non-specific binding, elution of the antibody-protein-DNA complex, de-crosslinking to release the DNA, and purification of the enriched DNA for downstream analysis.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
<a href="https://mbpinc.net/reagents/epigenetics/chip-kits/buffers">Buffers: </a>for immunoprecipitation, chromatin preparation, elution, and washing workflows. Includes Chromatin Shearing Buffer, MN Digestion Buffer, and Chromatin Wash Buffers, designed to support efficient chromatin cleanup, fragmentation, and recovery in ChIP assays
</li>
</ul>
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<h2>How to choose and use ChIP kits</h2>
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Decide between sonication-based and enzymatic chromatin shearing
Sonication shears chromatin mechanically into fragments typically 200&ndash;1000 bp through several optimized burst-and-cool cycles, while enzymatic kits use micrococcal nuclease (MNase) or similar enzymes to cleave chromatin at accessible linker regions. Each approach has different resolution characteristics and cell-type-specific optimization requirements.
Confirm your ChIP kit is compatible with your antibody and magnetic bead format
Most modern ChIP kits use magnetic protein A or protein G Dynabeads to capture the antibody-antigen complex during immunoprecipitation, and matching the bead type to the antibody species and isotype is important for efficient capture and low background.
Plan sonication optimization as a required step before your ChIP experiment
Fragment size after sonication, ideally 200&ndash;750 bp for most ChIP-seq applications, must be confirmed by gel electrophoresis or Bioanalyzer before proceeding, since fragment size directly affects both immunoprecipitation efficiency and the resolution of the resulting ChIP-seq data.
Include an input chromatin control and an isotype control antibody
Running an aliquot of the pre-immunoprecipitation chromatin as an input control normalizes for differences in chromatin accessibility across the genome, while an isotype-matched antibody control establishes background enrichment levels above which true binding sites can be distinguished.
Keep all steps after cell lysis cold to prevent protein degradation
Chromatin prepared for ChIP is sensitive to protease activity and non-specific interactions after formaldehyde fixation, and including protease inhibitors in all lysis and wash buffers, as well as keeping samples cold throughout, reduces background and preserves specificity of the immunoprecipitation.
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<h2>Specifications context</h2>
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Sonication requires careful optimization for each cell type and instrument, typically involving pilot experiments at varying pulse widths, total times, and rest intervals, with fragment size evaluated by gel electrophoresis after de-crosslinking to confirm the target range of 200&ndash;750 bp before a full ChIP experiment is run. As of 2026, low-input and ultra-low-input ChIP kits continue to expand access to ChIP experiments from limited cell numbers such as primary cell populations and rare clinical samples, while nano-ChIP and CUT&amp;RUN approaches provide complementary alternatives for very low cell inputs where classical ChIP requires too many cells.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at Molecular Biology Products Inc. (MBP) to get premium-quality ChIP reagents and buffers for your lab.

ChIP Kits

EZ Nucleosome DNA Prep Kit (20 Preps.)

D5220 by Zymo Research is the EZ Nucleosome DNA Prep Kit (20 preps) — isolates nucleosomal DNA from ≤10⁶ mammalian or yeast cells in under 45 minutes. Includes Micrococcal Nuclease, Atlantis dsDNase, and spin-column purification. Yield up to 25 µg; A260/A280 ≥1.8; 70–90% recovery for 75 bp–10 kb fragments.

Processing Volume

Purity

Sample Storage

Yield

Agarose

<h2>What is agarose for gel electrophoresis?</h2>
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Agarose is a linear polysaccharide derived from agar that, when dissolved in hot buffer and allowed to cool, forms a porous gel matrix whose pore size is determined by the agarose concentration. DNA and RNA molecules migrate through this matrix under an electric field at a rate inversely related to their size, allowing fragments of different lengths to be separated and visualized as distinct bands.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" role="presentation">Azura Agarose for routine electrophoresis of nucleic acids with low electroendosmosis (EEO) performance</li>
<li dir="ltr" role="presentation">Benchmark Agarose for PCR product analysis and high-quality nucleic acid separation</li>
<li dir="ltr" role="presentation">Benchmark Specialty Agarose for specialized gel applications requiring enhanced resolution and performance control</li>
<li dir="ltr" role="presentation">Standard Agarose for routine use in gel electrophoresis workflows</li>
<li dir="ltr" role="presentation">EZ Pack&trade; Agarose Tablets for agarose concentration control and convenient gel preparation&nbsp;</li>
</ul>
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<h2>How to choose agarose type and concentration</h2>
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Use standard agarose at 0.7&ndash;1% for routine PCR and restriction digest checks on larger fragments
A 0.7&ndash;1% standard agarose gel is commonly used for routine PCR product verification and restriction digest analysis when fragments are generally in the 1&ndash;10 kb size range.
Use 1.5&ndash;2% standard agarose for resolving smaller fragments in the 200 bp&ndash;2 kb range
Higher agarose concentrations from 1.5% to 2% are appropriate for resolving smaller PCR products, SNP genotyping amplicons, or restriction digests where size differences below 500 bp need to be clearly distinguished.
Use high-resolution agarose at 2&ndash;4% for very small fragments or close sizing
High-resolution agarose formulations can differentiate closely sized DNA fragments in the 50&ndash;500 bp range more effectively than standard agarose, making them useful for applications such as microsatellite or STR analysis.
Choose low-melting-point agarose when you need to recover DNA from gel slices
Low-melting-point (LMP) agarose can be melted at approximately 65&deg;C after electrophoresis, allowing DNA-containing gel slices to be processed more easily for downstream enzymatic reactions or purification workflows.
Confirm agarose purity (EEO grade) for sensitive applications
Electro-endosmosis (EEO) reflects agarose purity. Low-EEO agarose supports more consistent DNA migration and helps minimize contaminants that may interfere with downstream PCR amplification, restriction digestion, or other enzymatic applications.
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<h2>Specifications context</h2>
&nbsp;
Standard agarose gels are typically prepared by dissolving agarose powder in TAE or TBE buffer through heating, followed by cooling to approximately 55&deg;C before pouring into casting trays. Higher-concentration gels solidify more rapidly and generally benefit from prompt casting.
As of 2026, low-EEO agarose grades remain the preferred choice for sensitive applications such as Southern blotting, gel extraction followed by PCR, and restriction enzyme digestion workflows where contaminants can inhibit downstream reactions.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> and get premium agarose gel for your research today.

EZ Pack™ Agarose Tablets

EZ Pack™ Agarose Tablets (A2505) by MBP provide pre-measured molecular biology-grade agarose in tablet form for fast, reproducible, spill-free gel preparation — no weighing needed. Simply dissolve in TAE or TBE buffer by microwave. Available in A2505 and A2501 pack sizes. Stores at room temperature.

EZ Pack™ Agarose Tablets, pack of 1000 tablets (500g)

EZ Pack™ Agarose Tablets, pack of 200 tablets (100g)

EZ RNA Methylation™ Kit

EZ RNA Methylation™ Kit R5001 by Zymo Research combines RNA denaturation and bisulfite conversion in a single step, achieving &gt;99% cytosine conversion and &gt;80% RNA recovery from 0.5–1 µg input in 50 minutes. Converted RNA is compatible with RT-PCR, Sanger sequencing, next-generation sequencing library preparation, and microarray analysis for RNA m5C methylation profiling.

EZ RNA Methylation™ Kit (50 Preps.)

EZ RNA Methylation™ Kit (200 Preps.)

D5023-Protocol

EZ-96 DNA Methylation-Direct Kit

EZ-96 DNA Methylation-Direct Kit (D5022) by Zymo Research — 96-well shallow format, 192 total reactions. Direct bisulfite conversion from blood, tissue, cells, or FFPE without prior DNA extraction. Input: 10 cells to 10⁵ cells or 50 pg–2 µg DNA. Elutes in 30 µL. WGBS and targeted methylation ready.

Deep-well

EZ-96 DNA Methylation-Direct™ Kit (Shallow-well) (2 x 96 Rxns.)

EZ-96 DNA Methylation-Direct™ Kit (Deep-well) (2 x 96 Rxns.)

App Note with Tecan

EZ-96 DNA Methylation-Direct MagPrep

D5044 by Zymo Research — EZ-96 DNA Methylation-Direct MagPrep; 96-well magnetic bead bisulfite conversion; &gt;99.5% conversion; 50 pg–2 µg input; direct from blood, tissue, FFPE, LCM; ≥25 µL elution; ~4 hr; 4×96 rxns.