Brands

<h2>What are bisulfite conversion kits?</h2>
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Bisulfite conversion kits are all-in-one systems that bundle every reagent and column needed for the sodium bisulfite conversion workflow into a single, pre-optimized format, covering the denaturation and sulfonation steps, desulfonation with an alkali reagent, and a post-conversion cleanup step that removes the bisulfite and other reaction byproducts before the converted DNA is used in downstream analysis. This contrasts with standalone Bisulfite Conversion Reagents &amp; Buffers, which lets labs assemble their own optimized protocol from individual components.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Standard bisulfite conversion kits for routine DNA methylation analysis&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Combined transcriptomic and epigenetic studies with RNA and DNA methylation conversion kits</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">High-throughput 96-well bisulfite conversion kits for wider-scale screening applications</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Automation-compatible bisulfite kits for simplified workflow integration</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Magnetic bead-based bisulfite conversion kits for enhanced purification and recovery efficiency</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Direct bisulfite conversion kits for streamlined sample processing&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Rapid conversion kits for methylation analysis workflows that are time-sensitive&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Starter kits for validation and optimized setup of bisulfite conversion protocols</li>
</ul>
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<h2>How to choose a bisulfite conversion kit</h2>
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Match kit format to your downstream sequencing or PCR application
Kit versions optimized for WGBS work at larger input scales with library prep in mind, while RRBS-compatible kits often include restriction enzyme digestion components to enrich CpG-dense regions before conversion, and MSP-focused kits may include primers or protocol guidance for post-conversion methylation-specific PCR.
Check the kit's validated input DNA range against your sample type
Different bisulfite conversion kits are validated for different input DNA ranges, and kits validated for low-input or cell-free DNA samples specifically address the additional fragmentation and yield challenges that these sample types face during the harsh chemical conversion step.
Confirm the kit includes an unmethylated conversion control or specify one separately
A quality bisulfite conversion kit should provide, or clearly specify, an unmethylated control to run alongside samples, since tracking conversion efficiency across the kit's components helps catch kit-specific variability in conversion completeness between different lots or runs.
Consider kits with optimized FFPE protocols when working with formalin-fixed tissue
Formalin-fixed, paraffin-embedded tissue DNA is already fragmented before conversion; kits with FFPE-specific protocol variants include modifications like shortened incubation times and carrier reagents to minimize the further fragmentation this already-compromised input type would suffer under standard conversion conditions.
Evaluate throughput format options to match your sample volume
Some bisulfite conversion kits are available in spin column formats suited to individual sample processing, while plate-based or automation-compatible formats support higher-throughput sample sets, making format an important practical consideration beyond kit chemistry alone.
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<h2>Specifications context</h2>
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Bisulfite sequencing is widely regarded as the gold standard for DNA methylation analysis at single-base resolution, and a key advantage of using a complete kit over standalone reagents is that the included cleanup step reliably removes residual bisulfite and salt from the converted DNA, which would otherwise inhibit downstream PCR amplification and library preparation. As of 2026, enzymatic conversion kits (covered in Non-Bisulfite Kits &amp; Reagents) are increasingly positioned as an alternative that avoids the DNA damage introduced by chemical bisulfite treatment, though bisulfite conversion kits remain broadly used and well-validated across the largest range of downstream applications.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP and get high-quality bisulfite conversion kits for your lab.

Bisulfite Conversion Kits

DataSheet

MSDS

Protocol

App Note with Tecan

EZ-96 DNA Methylation-Gold MagPrep

D5042 by Zymo Research converts 96 DNA samples per plate to bisulfite form with &gt;99% efficiency using a two-stage protocol (98°C/10 min + 64°C/2.5 hrs) and magnetic bead cleanup. The EZ-96 DNA Methylation-Gold™ MagPrep (4×96 reactions) is validated for the Tecan Freedom EVO and compatible with Illumina EPIC arrays and WGBS. D5043 provides 8×96 reactions.

Applications

Conversion

Elution Volume

Equipment

Input

Processing Time

Recovery

Sample Source

Manufacturer

Shipping Condition

Storage Condition

EZ-96 DNA Methylation-Gold™ MagPrep (4 x 96 Rxns.)

EZ-96 DNA Methylation-Gold™ MagPrep (8 x 96 Rxns.)

D5007-Protocol

D5008-Protocol

EZ-96 DNA Methylation-Gold™ Kit

D5007 by Zymo Research is a 2 × 96 reaction EZ-96 DNA Methylation-Gold Kit using one-step thermal denaturation + bisulfite conversion at 64°C for 2.5 hours—enabling same-day processing. Achieves &gt;99% conversion efficiency, &gt;75% DNA recovery, 30 µl elution. Accepts 500 pg–2 µg input DNA per well. Compatible with WGBS, RRBS, MSP, pyrosequencing, and Illumina methylation arrays.

EZ-96 DNA Methylation-Gold™ Kit (2 x 96 Rxns.) - Shallow Well

EZ-96 DNA Methylation Gold™ Kit (2 x 96 Rxns.) - Deep Well

D5046/D5047-MSDS

D5046-E/D5047-E-MSDS

D5046/D5047-Protocol

D5046-E/D5047-E-Protocol

App Note with Hamilton

EZ-96 DNA Methylation-Lightning MagPrep

D5046 by Zymo Research processes 96 samples per plate via magnetic beads, achieving &gt;99.5% bisulfite conversion in ~68 minutes with 100 pg–2 µg DNA input. The EZ-96 DNA Methylation-Lightning™ MagPrep (4×96 reactions) is validated for the Tecan Freedom EVO and compatible with Illumina EPIC arrays, WGBS, and targeted BS-Seq. D5047 provides 8×96 reactions for larger cohorts.

EZ-96 DNA Methylation-Lightning™ MagPrep CE-IVD (4 x 96 Rxns.)

EZ-96 DNA Methylation-Lightning™ MagPrep (8 x 96 Rxns.)

EZ-96 DNA Methylation-Lightning™ MagPrep CE-IVD (8 x 96 Rxns.)

EZ-96 DNA Methylation-Lightning™ MagPrep (4 x 96 Rxns.)

D5032-Data Sheet

D5032-MSDS

D5032-Protocol

D5033-Protocol

D5032-App Note (Hamilton)

EZ-96 DNA Methylation-Lightning™ Kit

EZ-96 DNA Methylation-Lightning™ Kit (D5032) by Zymo Research converts 100 pg–2 µg DNA with &gt;99.5% bisulfite conversion efficiency in 1.5 hours across a 96-well plate format (2×96 rxns/kit). &gt;80% recovery, &gt;50 bp fragments, Hamilton-automation compatible. Available from MBP Inc.

EZ-96 DNA Methylation-Lightning™ Kit (Shallow-Well) (2 x 96 Rxns.)

EZ-96 DNA Methylation-Lightning™ Kit (Deep-Well) (2 x 96 Rxns.)

D5004-Protocol

D5003-Protocol

D5003-App Note

EZ-96 DNA Methylation™ Kit

D5003 by Zymo Research is a 2 × 96 reaction bisulfite conversion kit in shallow-well format for high-throughput DNA methylation analysis. Converts &gt;99% of unmethylated cytosines to uracil; protects &gt;99% of methylated cytosines; &gt;80% DNA recovery in ≥30 µl. Accepts 500 pg–2 µg input DNA per well. Compatible with MSP, WGBS, RRBS, pyrosequencing, and Illumina methylation arrays.

EZ-96 DNA Methylation™ Kit (Shallow-Well) (2 x 96 Rxns.)

EZ-96 DNA Methylation™ Kit (deep-well) (2 x 96 Rxns.)

App Note

EZ-96 DNA Methylation™ MagPrep

The EZ-96 DNA Methylation™ MagPrep (D5040) by Zymo Research performs 96-well magnetic bead-based bisulfite conversion of 500 pg–2 µg DNA with &gt;99% conversion efficiency and &gt;70% recovery. Processing time 12–16 hours; compatible with PCR, sequencing, microarrays, and NGS. Available in 4 × 96 and 8 × 96 reaction formats.

EZ-96 DNA Methylation™ MagPrep (4 x 96 Rxns.)

EZ-96 DNA Methylation™ MagPrep (8 x 96 Rxns.)

Excel Scientific

EZ-Pierce

EZ-Pierce (EZP-100) by MBP is a pierceable adhesive plate sealing film that allows clean well access via pipet tip or dispensing needle without removing the full seal. Non-sterile (EZP-100) and sterile (EZPS25) variants available. Ships ambient; store at room temperature.

EZ-Pierce™, 100/Cs

EZ-Pierce™, Sterile,50/Cs

Devices & Instruments

EZ-Vac Vacuum Manifold

EZ-Vac Vacuum Manifold (S7000) by Zymo Research — processes 1–20 Zymo-Spin columns simultaneously under vacuum (&ge;400 mm Hg). Linear polyethylene body, one-way Luer-Lock stopcocks, polypropylene vacuum connector. Compatible with most Zymo nucleic acid purification kits.

Capability

Compatibility

Composition

EZ-Vac96 Vacuum Manifold

EZ-Vac96™ Vacuum Manifold S7003 by Zymo Research — processes up to 96 samples simultaneously using vacuum-driven column flow, eliminating centrifugation. Compatible with ZymoPURE™ 96 Plasmid Miniprep Kit (D4214, D4215); requires vacuum pump capable of up to 300 mmHg and 6.5 mm ID tubing. Chemical-resistant construction with external catch flask. Order via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or contact <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.

Required Equipment

PCR & qPCR Film

EZcap™ FoilStrips™, 6 Strips per Sheet, 1X8 Rows per Strip

EZcap™ FoilStrips™ (F2CS1X8300) by MBP are precision-cut aluminum foil strip seals for PCR 8-tube strips or single rows of 96-well PCR or deep-well storage plates. 6 strips/sheet, 1&times;8 rows/strip. Remove easily with no splash back. Single SKU. Ships ambient.

Application

Temperature Range

EZcap™ PCR FilmStrips™, 8 Strips per Sheet, 1X8 Rows per Strip, For sealing a PCR 8-tube strip or a single row of a 96-well plate, 400/Pack

EZcap™ PCR FilmStrips™ (US-CS1X8-400) by MBP are adhesive peel-and-stick alternatives to PCR strip-caps — no sore thumbs. 8 strips/sheet, 1&times;8 rows/strip; compatible with PCR 8-tube strips and single 96-well plate rows. 400 strips/pack. Ships ambient; single SKU.

<h2 data-section-id="185nm5c" data-start="0" data-end="38">What is classic cell culture media?</h2>
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Classic cell culture media refers to the foundational basal medium formulations that have underpinned mammalian cell culture for decades, including DMEM (Dulbecco's Modified Eagle Medium), MEM (Minimum Essential Medium), RPMI 1640, Ham's F-10, Ham's F-12, IMDM (Iscove's Modified Dulbecco's Medium), and DMEM/F-12. These media provide the amino acids, vitamins, glucose, salts, buffering agents, and other nutrients required to support cell survival and growth in vitro. They form the basis of many modern specialized, serum-free, and advanced media formulations used in academic research, biotechnology, pharmaceutical development, and biomanufacturing.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
&nbsp;Availability of both low and high carbohydrate levels of balanced basal formulations for the distinct metabolic requirements of diverse mammalian cell lines.
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Maintenance of precise physiological pH during long-term incubation cycles using optimized buffering configurations with integrated chemical stabilizers&nbsp;
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<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Nutrient-dense cell media supplemented with vitamins and essential amino acids to support&nbsp; hybridomas and rapidly proliferating continuous cultures
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<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Optimized serum reduction along with enhanced cell attachment using&nbsp;Multi-purpose 1:1 formulation hybrids&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Salt solutions supplemented with essential minerals to facilitate enzyme preparation, cell washing, and reliable short-term osmotic maintenance.
</li>
</ul>
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<h2 data-section-id="1blio5n" data-start="931" data-end="964">How to choose a classic medium</h2>
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DMEM vs MEM
DMEM contains up to four times the amino acids and vitamins found in MEM, along with higher glucose concentrations, making it suitable for cell types with elevated nutrient demands. It is widely used for primary cells, stem cells, and many transformed mammalian cell lines. MEM, based on the original Eagle's formulation, remains a common choice for standard monolayer culture and is available with different salt formulations to support various applications.
RPMI 1640 for suspension and hematopoietic culture
RPMI 1640 was originally developed for culturing human leukemic cells and remains the preferred medium for lymphocytes, hybridomas, and other hematopoietic cell types. Its formulation includes glutathione and elevated levels of biotin, vitamin B12, and PABA. Although designed for suspension cultures, RPMI 1640 also supports a wide range of adherent cell lines when appropriately supplemented.
Ham's F-10/F-12 for CHO and specialized applications
Ham's nutrient mixtures were developed for CHO cells and other specialized cell types. Their formulations include additional nutrients such as zinc, putrescine, hypoxanthine, and thymidine, making them useful for hepatocyte culture, toxicity testing, viral fusion studies, and other specialized applications. These media can be used with or without serum depending on the experimental requirements.
DMEM/F-12 for complex, rich-medium applications
DMEM/F-12 combines the nutrient-rich composition of DMEM with the specialized components found in Ham's F-12. The resulting formulation supports demanding cell types including MDCK cells, glial cells, fibroblasts, and endothelial cells. It is also widely used as the foundation for many serum-free and advanced culture systems.
IMDM for high-density rapid proliferation
IMDM is an enriched derivative of DMEM that contains additional amino acids, vitamins, HEPES buffer, sodium pyruvate, and potassium nitrate. Its enhanced nutrient profile makes it particularly suitable for rapidly proliferating and high-density cultures such as Jurkat cells, COS-7 cells, and macrophages.
Liquid vs powder format
Liquid classic media are supplied ready for use after the addition of serum and required supplements. Powdered media offer lower shipping costs and greater flexibility for high-volume laboratories but require reconstitution, sodium bicarbonate addition, and sterile filtration before use.
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<h2 data-section-id="1fljvas" data-start="4382" data-end="4407">Specifications context</h2>
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Classic media are manufactured according to their original published formulations, with only stability-related modifications where necessary. Most liquid formulations contain sodium bicarbonate as the primary buffering system and require incubation in a 5&ndash;10% CO₂ atmosphere to maintain physiological pH. Powdered formulations require reconstitution, sodium bicarbonate supplementation, and sterile filtration before use.
As of 2026, DMEM, DMEM/F-12, and RPMI 1640 remain the most widely used classic cell culture media across academic, biotechnology, pharmaceutical, and biomanufacturing laboratories. IMDM continues to gain popularity for high-density culture and bioprocessing applications.
For recombinant growth factors and cytokines used alongside classic media, see Recombinant Proteins. For supplementation reagents such as GlutaMAX, NEAA, sodium pyruvate, and HEPES, see Cell Culture Supplements &amp; Additives. Browse Mammalian Cell Culture for the broader category.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get optimized classic media for your lab.