Brands

<h2 data-section-id="xs82dy" data-start="121" data-end="167">What are additional immortalization agents?</h2>
&nbsp;
Additional immortalization agents cover recombinant lentiviral constructs delivering genes other than TERT or SV40 large T antigen used to bypass replicative senescence in primary cells, most notably HPV-16 E6/E7 (which inactivate p53 and Rb similarly to SV40T but are derived from human papillomavirus), Bmi1 (a polycomb group protein that represses the p16/CDKN2A senescence pathway), cMyc, CDK4, and HOXA/HOXB family genes. Related entities include p16/CDKN2A pathway repression (Bmi1), keratinocyte and epithelial cell immortalization (HPV E6/E7), polycomb group signaling, and combination immortalization strategies.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Lenti-HPV-16 E6/E7 degrades Rb and p53 for enhanced cell immortalization.</li>
<li dir="ltr" role="presentation">Lenti-Myc T58A &amp; Ras V12 enhance transformation and proliferation assays with high-titer mutant oncogenes&nbsp;</li>
<li dir="ltr" role="presentation">Lenti-p53 &amp; Rb siRNA Virus extends cellular longevity and targets the knockdown of tumor suppressors</li>
<li dir="ltr" role="presentation">Lenti-HOX Series (RFP Tagged) for lineage-specific expansion and includes high-titer vectors like HOXB8, HOXA9, and HOXA10 vectors.</li>
<li dir="ltr" role="presentation">EBV Virus (Wild-Type) for immortalization workflows of B-lymphocytes&nbsp;</li>
<li dir="ltr" role="presentation">Lenti-CDK4 &amp; Bmi1 Virus helps maintain proliferative capacity using cell mediators</li>
</ul>
&nbsp;
<h2 data-section-id="1acs4rs" data-start="1029" data-end="1087">How to choose additional immortalization agent products</h2>
&nbsp;
HPV E6/E7 for epithelial and keratinocyte immortalization
HPV-16 E6 and E7 proteins inactivate p53 and Rb respectively, similar in net effect to SV40 large T antigen but derived from a different viral source; E6/E7-based immortalization is particularly well-documented for keratinocytes and other epithelial cell types, and is sometimes preferred over SV40T for these lineages based on published precedent.
Bmi1 for stem and progenitor cell types
Bmi1 represses the p16/CDKN2A senescence pathway through its role in the polycomb repressive complex, and has been used to extend the proliferative capacity of hematopoietic and neural stem/progenitor cells in research settings; confirm the specific cell type has documented precedent for Bmi1-based extension before relying on it as a primary immortalization strategy.
cMyc and CDK4 as proliferation-driving factors
cMyc and CDK4 overexpression can drive proliferation and contribute to immortalization, often used in combination rather than as standalone immortalization agents; review the specific combination strategy in the source literature before ordering individual components.
E6/E7 separate vs. fused constructs
HPV E6/E7 may be delivered as separate genes or as a fused open reading frame; confirm which configuration a published protocol specifies, since this can affect relative expression levels of the two proteins.
&nbsp;
<h2 data-section-id="1fljvas" data-start="3287" data-end="3312">Specifications context</h2>
&nbsp;
Additional immortalization agent lentiviruses are typically supplied at titers around 1x10^8 TU/mL, shipped on dry ice and stored at -80&deg;C, with antibiotic or fluorescent-antibiotic fusion selection markers consistent with other immortalization lentivirus products. Pack sizes for academic labs commonly consist of small-volume aliquots sufficient for multiple transduction attempts, with constructs validated by sequencing and tested free of common contaminants.&nbsp;
This sub-category sits within MBP's cell immortalization catalog alongside TERT overexpression viruses and SV40T antigen overexpression viruses. Labs constructing custom combination vectors can also review MBP's cloning reagents. MBP's specialist team can help confirm which immortalization agent has documented precedent for a specific cell type before order placement.
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today to book high-quality immortalization vectors for your lab.

Additional Immortalization Agents

MSDS

Lentiviral Vector Amplification - Protocol

Lentivector Packaging - Protocol

Lentivirus Infection - Protocol

Cell Immortalization - Handbook

Cell Immortalization Reagents - FAQ

E1A-E1B Lentivirus, High Titer

Unit Quantity

Application

Expression System Type

Vector Map

Note

Caution

Shipping Condition

EBV-Safety Data Sheet

EBV Virus (Wild-Type)

Unpacking and Storage Instructions

Laboratory Oven

<h2>What Are Laboratory Ovens?</h2>
&nbsp;
Laboratory ovens are electrically heated, thermally insulated chambers that maintain a defined temperature for drying, heating, curing, sterilizing, or processing solid samples and materials placed on internal shelves. Unlike water baths or dry block heaters that heat liquid samples in tubes, laboratory ovens process bulk materials, glassware, powders, porous materials, and large samples that cannot fit in tube-based incubators. The two principal oven types are forced air convection ovens (using a fan to circulate heated air at 300-500 m3/h for uniform, rapid temperature distribution and moisture removal) and vacuum drying ovens (sealing the chamber and using an external vacuum pump to reduce pressure below 133 Pa, dramatically lowering the boiling point of water and solvents to enable low-temperature drying at 30-60 degrees C for heat-sensitive materials). Choose forced air convection for most drying, sterilization, and curing applications at temperatures above 50 degrees C. Choose vacuum ovens when samples are heat-sensitive, oxygen-sensitive, or require solvent removal at temperatures below 60 degrees C.
&nbsp;
<h2 dir="ltr">What You Will Find:</h2>
&nbsp;
<a href="https://mbpinc.net/equipments/temperature-control-equipment/laboratory-oven/forced-drying-ovens" title="Forced Drying Ovens:">Forced Drying Ovens:</a>
<ul>
<li>High-speed fans ensure rapid temperature recovery and perfect heat uniformity across every shelf.</li>
<li>Stainless steel interiors provide maximum durability and are easy to keep clean and organized.</li>
</ul>
<a href="temperature-control-equipment/laboratory-oven/vacuum-drying-ovens" title="Vacuum Drying Ovens:">Vacuum Drying Ovens:</a>
<ul>
<li>Reduced-pressure environments allow for safe moisture removal at lower temperatures for heat-sensitive materials.</li>
<li>Digital safety controls protect your samples from oxidation and prevent accidental overheating.</li>
</ul>
&nbsp;
<h2>How to Choose a Laboratory Oven</h2>
&nbsp;
Forced air vs. vacuum
Forced air convection ovens use a fan to distribute heated air uniformly throughout the chamber, providing rapid temperature recovery after door opening and temperature uniformity of plus or minus 1-5 degrees C across the chamber. They are suitable for drying glassware and labware after washing, dehydrating soil or biological specimens for moisture content analysis, dry heat sterilization at 160-180 degrees C, polymer curing, aging tests, and annealing of soft materials. Vacuum drying ovens remove air and moisture from a sealed chamber by vacuum pump, enabling drying at 30-200 degrees C at pressures below 133 Pa. They are required for heat-sensitive pharmaceuticals and APIs, porous materials with moisture trapped in internal cavities, electronic components sensitive to oxidation, and materials processed under oxygen-free conditions.
Temperature range
Standard laboratory ovens cover ambient plus 10 degrees C to 250-300 degrees C for most research and quality control applications. Forced air drying ovens such as the Labtron LFDO-A18 specify RT plus 10 degrees C to 300 degrees C with 225 L capacity. High-temperature ovens reach 350-500 degrees C for ceramics and materials research. Vacuum drying ovens typically cover RT plus 10 degrees C to 200 degrees C; the lower maximum reflects the glass or stainless steel chamber design and silicone door seal constraints. Avoid specifying an oven with a maximum temperature far above the required process temperature: ovens are less stable and less uniform at temperatures well below their ceiling.
Chamber volume and shelf capacity
Oven chamber volume is specified in liters (15 L to 500 L for benchtop models; floor-standing models up to 1,000 L) or cubic feet (0.5 to 18 cu. ft.). Shelf count, shelf dimensions, and maximum load per shelf (kg per shelf) determine actual sample throughput. Standard benchtop laboratory ovens have 2-4 adjustable stainless steel perforated shelves. Vacuum ovens have 2-4 heated shelves in a smaller chamber (typically 0.6-3 cu. ft.) sealed for pressure operations.
Temperature uniformity
Uniformity (variation across the chamber at setpoint) for forced air ovens is typically plus or minus 2-5 degrees C for standard models and plus or minus 0.5-2 degrees C for precision models. Uniformity is improved by forced air circulation but remains poorer than dry block heaters or water baths because the larger chamber volume contains thermal gradients even with fan circulation. Placing samples in the center of the chamber away from walls and heating elements reduces exposure to thermal gradients.
Safety and ventilation
Standard laboratory ovens are not rated for flammable solvent applications and must not be used with volatile organic solvents (ethanol, acetone, diethyl ether): solvent vapors can ignite on the heating elements or fan motor. For flammable solvent work, explosion-proof or flammable-material-safe ovens with ATEX-rated motors and all-inert internal components are required. Over-temperature protection (safety thermostat at a fixed cutoff above the setpoint) and over-current protection are standard safety features on research-grade laboratory ovens.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Forced air ovens achieve temperature uniformity of plus or minus 2-5 degrees C across the chamber when the fan is in operation; uniformity degrades toward the chamber corners and near the heating elements. Vacuum drying ovens at 0.098 MPa vacuum (133 Pa, near full vacuum for benchtop models) reduce the boiling point of water from 100 degrees C to approximately 40-50 degrees C, enabling thorough drying of heat-sensitive materials at setpoints as low as 40 degrees C without thermal degradation. Dry heat sterilization of glassware in a forced air oven is typically performed at 160-180 degrees C for 2-4 hours, destroying bacterial spores and endotoxin more effectively than autoclave steam sterilization at equivalent temperatures. Forced air laboratory ovens with 4-zone heated air jacket designs, microprocessor PID controls, programmable multi-segment ramp-and-hold profiles, and USB data logging are the standard specification for new research and pharmaceutical laboratory installations.&nbsp;
&nbsp;
Ready to upgrade your thermal capacity? Explore our laboratory oven range and <a href="https://mbpinc.net/contact-us">reach out to the MBP team</a> for a friendly quote today.

Vacuum Ovens

Quick disconnect fitting (pre-installed at vent port)

ECO 150C Vacuum Drying Oven with LED Lights

A step-by-step guide to installing and calibrating the AT Vacuum Oven. Learn the correct setup process and calibration tips to ensure optimal performance.

AT Vacuum Oven Installation and Calibration

AT09-50 by Across International is the ECO 150C Vacuum Drying Oven with LED Lights — a 0.9 cu ft (approx. 25.5 L) stainless steel chamber rated to 150 °C under &lt;500 micron vacuum, with 3-sided 1,500 W heating, microcomputer PID control, and a 1–9,999 min timer. Includes 4 aluminum shelves (expandable to 8), a KF25 vacuum flange, dual-layer tempered glass window, and LED interior lighting. Available in 110V and 220V. Suitable for solvent removal, degassing, pharmaceutical drying, and electronic component processing.

Manufacture

ECO 150C 0.9 Cu Ft Vacuum Drying Oven with LED Lights

ECO 150C 1.9 Cu Ft Vacuum Drying Oven with LED Lights

ECO 150C 3.2 Cu Ft Vacuum Oven 3 Heated Shelves & LED Lights

ECO UL 150C 14 Shelf Max 5 CF Vacuum Oven w/ LED Lights

<h2>What is Chemicals as a reagent category?</h2>
&nbsp;
Chemicals covers the broad range of chemical and biochemical reagents used across molecular biology, cell biology, biochemistry, and general laboratory work: biochemically relevant compounds like vitamins, buffer salts, amino acids, and enzyme substrates; general laboratory chemicals including solvents, acids, bases, and inorganic salts; and research-grade specialty chemicals for specific applications. Unlike application-specific categories such as Epigenetics or Electrophoresis, which organize reagents by research workflow, Chemicals organizes by the nature of the material itself.
&nbsp;
<h2 dir="ltr">What you'll Discover:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/chemicals/biochemical-reagents" title="Biochemical Reagents:">Biochemical Reagents:</a> High-quality metabolic inducers such as IPTG (G116), L-Arabinose (A2001-ZYM), and Cumate (CH065), along with essential buffers and enzyme stabilizers like TRIS (CH059), DTT (G025), and PMSF (G034).
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/chemicals/inorganic-reagents-and-compounds" title="Inorganic Reagents &amp; Compounds:">Inorganic Reagents &amp; Compounds:</a> Ultra-pure essential chemical substances like Sodium Azide (CH052) designed for accurate laboratory buffer enhancement and sample conservation methodologies.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/chemicals/detergents-and-surfactants" title="Detergents &amp; Surfactants:">Detergents &amp; Surfactants:</a> Enhanced cell lysis and denaturing agents featuring 20% SDS (CH100) and concentrated 10X Tris-Glycine-SDS Buffer (CH125) for protein electrophoresis setup.
</li>
</ul>
&nbsp;
<h2>How to navigate Chemicals</h2>
&nbsp;
Start with Biochemical Reagents for biologically relevant compounds
Biochemical Reagents covers the subset of the catalog most relevant to molecular and cell biology workflows: vitamins and cofactors, buffer salts and ready-to-use buffer solutions, amino acids, and other compounds with specific roles in biological systems.
Check purity grade requirements before ordering a research chemical
Research-grade chemicals vary in purity from technical grade to ACS-certified, HPLC-grade, and molecular biology grade, with the appropriate grade depending on how the chemical is used; cell culture and enzymatic applications generally require higher purity than non-biological analytical or general lab use.
Confirm whether your chemical needs are better served by a specialty category
Some research chemicals belong more naturally in application-specific sections of the catalog, such as chromatin immunoprecipitation buffers in Epigenetics or gel running buffers in Electrophoresis, while general-purpose chemicals and biochemical reagents not tied to a single workflow type belong in this Chemicals branch.
Consider bulk ordering for high-consumption research chemicals
Common research chemicals like buffer salts, solvents, and vitamins are used in large quantities by active research labs, and bulk pricing can represent meaningful savings on frequently replenished reagents.
Look for certificates of analysis when accuracy matters for your application
Quantitative applications, including analytical standards, enzyme kinetics studies, and defined cell culture supplementation, require chemicals with documented lot-specific purity and concentration, typically confirmed by certificate of analysis provided with each lot.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Research-grade purity designations for laboratory chemicals follow widely adopted convention systems including ACS grade (meets American Chemical Society purity specifications), HPLC grade (verified purity for chromatographic applications), and molecular biology grade (certified free from nuclease, protease, and other activities that would interfere with molecular biology applications). As of 2026, pre-made, ready-to-use solutions for common biochemical reagents like PBS, HEPES buffer, and Tris-HCl continue to see growing adoption over powder or concentrated formats in labs prioritizing convenience and reproducibility over reagent cost per use.
<a href="https://mbpinc.net/contact-us">Reach out to the friendly team at MBP</a> now to get a quote for your particular research needs.

Chemicals

EDTA, Disodium Salt, Dihydrate

Storage Condition

Guarantee

Shipping condition

<h2>What Are Electrophoresis Systems?</h2>
&nbsp;
An electrophoresis system is the complete apparatus for casting and running gels, comprising the electrophoresis tank, gel casting tray, sample loading combs, electrode assembly, and buffer reservoirs - used in combination with an external power supply to drive biomolecule separation. Horizontal submarine systems submerge the agarose gel beneath electrophoresis buffer (TAE or TBE), connecting the gel to platinum or carbon electrodes at each end. Applying 80-120 V drives negatively charged DNA or RNA toward the positive electrode, with smaller fragments migrating further. Vertical systems clamp two glass or plastic plates around a poured polyacrylamide gel, connecting the upper and lower buffer chambers for protein or oligonucleotide separation. Match the electrophoresis system format (mini vs. standard vs. wide), tray size, well count, and buffer capacity to your sample throughput and fragment size range.
&nbsp;
<h2 dir="ltr">What You Will Find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Electrophoresis Tanks &ndash; High-durability, single-piece molded tanks designed for leak-proof runs and optimal buffer circulation.
</li>
<li dir="ltr" aria-level="1">
Standard 300V Power Supplies &ndash; Full-featured digital units with advanced programming, ideal for high-performance and high-capacity laboratory runs.
</li>
<li dir="ltr" aria-level="1">
Mini 300V Power Supplies &ndash; Ultra-compact, space-saving units engineered for reliable, routine electrophoresis within a smaller bench footprint.
</li>
</ul>
&nbsp;
<h2>How to Choose an Electrophoresis System</h2>
&nbsp;
Format: mini, standard, or wide
Mini electrophoresis systems with 7 x 8 cm gel trays accommodate 8-16 samples per gel and run in 20-35 minutes at 100 V in TAE, making them the fastest option for quick PCR band verification. Standard systems with 10 x 14 cm trays hold 20-30 samples and are the workhorse format for most molecular biology labs. Wide and large-format systems with gel trays of 20 x 20 cm or greater, or multi-row comb configurations, allow 50-100+ samples per gel for high-throughput applications such as colony PCR screening. Multi-format systems that accept interchangeable trays in the same tank provide the best flexibility.
Horizontal vs. vertical configuration
Horizontal agarose gels are the standard for nucleic acid work: they are easy to cast, accommodate large well counts, and can separate fragments of 100 bp to 20 kb in a single run with appropriate agarose concentration. Vertical polyacrylamide gels provide sharper band resolution (under 5 bp for sequencing gels; under 1 kDa for SDS-PAGE proteins) and are required for western blotting workflows. Mini-PROTEAN-style vertical systems (Bio-Rad format) are the most widely referenced standard; confirm cassette compatibility when purchasing from alternative manufacturers.
Well volume and comb specifications
Standard well combs create wells of 5-10 mm width, accommodating 10-30 microliters of sample in 1x loading buffer. Preparative combs create a single wide well (20-50 mm) for running large-volume samples ahead of gel extraction. Comb tooth count is specified per tray configuration; 8, 12, 15, 20, or 24-tooth combs are common for standard formats. Confirm that the comb thickness (0.75 mm or 1.0 mm) matches the gel cassette spacer width for vertical PAGE systems.
Buffer compatibility
TAE (1x: 40 mM Tris, 20 mM acetic acid, 1 mM EDTA, pH 8.3) is the standard buffer for agarose gels used in cloning and routine DNA analysis; DNA migrates approximately 10% faster in TAE than TBE. TBE (1x: 89 mM Tris, 89 mM boric acid, 2 mM EDTA) gives sharper resolution of fragments below 1 kb and is standard for polyacrylamide gels. MOPS-formaldehyde buffer is used for denaturing RNA gels. Never mix buffer types between chambers during a run.
Integrated vs. separate power supply
Some compact electrophoresis systems include an integrated power supply, simplifying setup and reducing bench footprint. Separate, dedicated power supplies provide more precise voltage control, current monitoring, and the ability to run multiple gel units simultaneously from one supply. Dedicated power supplies rated at 300 V and 400 mA allow running 2-3 mini gel units or 1-2 standard units simultaneously.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Buffer capacity (mL) determines how long a gel can run at standard voltage without buffer depletion; standard horizontal systems hold 200-500 mL buffer. For very long runs exceeding 90 minutes (e.g., for large DNA fragment separation or PFGE), buffer recirculation or mid-run buffer exchange is required to prevent ion depletion and pH shift. Electrode material -- platinum wire (corrosion-resistant, long lifespan) versus carbon rod (lower cost) - affects current delivery consistency over the electrode lifetime; platinum electrodes are standard on research-grade systems. UV-resistant plastic (UV-blocking polycarbonate or polypropylene) gel trays are standard on new electrophoresis systems to prevent UV degradation during UV transilluminator use.&nbsp;
&nbsp;
Don&rsquo;t let your samples drift. Browse our electrophoresis hardware and <a href="https://mbpinc.net/contact-us">hit up the MBP team</a> for a custom quote today.

Electrophoresis System

Biologix® Electrophoresis Tank Gel

ELECTROPHORESIS TANK, 1 PIECE/PACK

Biologix® Electrophoresis Tank Gel, 1 Piece/Pack

Brand

Model

ELECTROPHORESIS TANK, GEL DIMENSION:70X70MM&70X100MM 1 PIECE/PACK

ELECTROPHORESIS TANK, GEL DIMENSION:100X70MM&100X100MM 1 PIECE/PACK

<h2 dir="ltr">What Are Specialized Cloning Vectors &amp; Viral Tools?</h2>
 Standard lentiviral and retroviral expression vectors meet the needs of most overexpression and knockdown workflows, but many cell biology and translational research questions demand purpose-engineered constructs with specialised functions &mdash; subcellular localisation, multi-gene co-expression, or conditional activation. Select the format best suited to your experimental requirements.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/dna/cloning-services-products/specialized-cloning-vectors/-virus/organelle-labeling-viruses">Organelle Labeling Viruses: </a>for live-cell visualization and functional pathway analysis of cellular structures in mammalian systems
</li>
<li dir="ltr" aria-level="1">
Targeted fluorescent labeling of specific cellular structures using viral delivery.
</li>
<li dir="ltr" aria-level="1">
Stable expression of organelle-specific markers for long-term live-cell imaging.
</li>
<li dir="ltr" aria-level="1">
Compatible with fluorescence microscopy for dynamic cellular imaging applications.
</li>
<li data-section-id="8k7pm2" data-start="506" data-end="603" data-is-last-node="">Suitable for studying organelle organization, transport, and cellular function in living cells.</li>
</ul>
<h2 dir="ltr"> How to Choose Specialized Cloning Vectors</h2>
 Subcellular Targeting Needs Organelle-targeted fluorescent labeling lentiviral particles deliver fluorescent protein constructs targeting mitochondria, endoplasmic reticulum, nucleus, Golgi, lysosome, peroxisome, plasma membrane, autophagosome, and cytoskeleton &mdash; providing a stable alternative to chemical dyes for live-cell imaging. Multi-Gene Co-Expression Bicistronic vectors using IRES or 2A self-cleaving peptide sequences enable co-expression of two genes (e.g., a gene of interest and a fluorescent marker) from a single transcript or vector. Conditional Expression Requirements Conditional DIO/FLEX vectors restrict transgene expression to Cre-expressing cells, essential for circuit-specific neuroscience research and in vivo conditional gene expression studies. Temporal Control Needs Inducible constructs (Tet-On/Tet-Off systems) provide doxycycline-regulated transgene activation for experiments requiring temporal control over expression timing. Delivery Format Most specialised constructs are available as lentiviral particles for stable, long-term expression in both dividing and non-dividing cell types, including primary cells and in vivo models. 
<h2 dir="ltr"> Specifications Context</h2>
&nbsp;
Traditional subcellular organelle imaging relies on chemical dyes (e.g., MitoTracker, LysoTracker, ER-Tracker) or antibody-based immunofluorescence in fixed cells; however, chemical dyes are transient and may exhibit cytotoxicity at working concentrations, while antibody-based methods are limited to endpoint imaging. Viral organelle-targeted fluorescent expression systems enable stable, genetically encoded labeling for long-term live-cell imaging and dynamic subcellular tracking. These constructs are supplied in plasmid DNA and pre-packaged viral formats and support applications including organelle dynamics, trafficking studies, and multicolor imaging workflows. Specialized cloning vectors and viral tools are sourced from established manufacturers, including Applied Biological Materials (ABM), and product availability reflects MBP&rsquo;s catalogue as of mid-2026.
&nbsp;
<a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact the MBP team </a>today and get specialized cloning vectors for your lab.