Zymo Research

<h2>What are epigenetics enzymes and inhibitors?</h2>
&nbsp;
Epigenetics enzymes and inhibitors are the molecular tools for directly studying or modulating the enzymatic machinery that writes, erases, or reads epigenetic marks. The major classes include: DNA methyltransferases (DNMT1, DNMT3A, DNMT3B), which catalyze the addition of a methyl group to cytosine; TET family enzymes (TET1, TET2, TET3), which oxidize 5-methylcytosine to 5-hydroxymethylcytosine; histone acetyltransferases (HATs) and histone deacetylases (HDACs), which add and remove acetyl groups from histone lysine residues; and histone methyltransferases (HMTs) including EZH2, DOT1L, and G9a. Small-molecule inhibitors of HDAC and DNMT enzymes are the most clinically and experimentally advanced of these tools.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Micrococcal nuclease for nucleosome mapping and controlled chromatin digestion applications</li>
<li dir="ltr" role="presentation">DNA shearing enzymes for consistent fragmentation of DNA in sequencing and epigenetic workflows</li>
<li dir="ltr" role="presentation">DNA degradation enzymes for controlled breakdown and analysis of nucleic acids&nbsp;</li>
<li dir="ltr" role="presentation">CpG methylase enzymes for targeted DNA methylation (CpG-specific )&nbsp; in epigenetic modification studies</li>
<li dir="ltr" role="presentation">5-hmC glucosyltransferase for detection and labeling of hydroxymethylcytosine in DNA</li>
<li dir="ltr" role="presentation">SAM (S-adenosylmethionine) cofactors for supporting enzyme activity and methylation reactions</li>
<li dir="ltr" role="presentation">DNA digestion enzymes for genomic DNA processing workflows</li>
<li dir="ltr" role="presentation">dsDNA nuclease systems for controlled cleavage and analysis of double-stranded DNA</li>
</ul>
&nbsp;
<h2>How to choose epigenetics enzymes and inhibitors</h2>
&nbsp;
Choose DNMT inhibitors to globally reduce or deplete DNA methylation in cells
Nucleoside analog DNMT inhibitors like 5-azacytidine and decitabine incorporate into DNA and trap DNMT1, preventing maintenance methylation and causing passive demethylation through DNA replication, while newer non-nucleoside DNMT inhibitors are in development for more selective, non-genotoxic methylation reduction.
Choose HDAC inhibitors to globally increase histone acetylation
HDAC inhibitors including pan-HDAC hydroxamates like vorinostat (SAHA) or class-selective compounds reduce histone deacetylase activity and cause hyperacetylation of histone lysine residues, broadly promoting gene expression at silenced loci. HDAC inhibitors are the most clinically advanced class of epigenetic therapeutics, with multiple FDA-approved compounds.
Use TET enzyme preparations for in vitro 5-hmC oxidation studies
Purified TET1, TET2, or TET3 enzyme preparations allow controlled in vitro oxidation of 5-methylcytosine to 5-hydroxymethylcytosine and further oxidation products, useful for generating defined 5-hmC-containing substrate DNA or studying TET enzyme activity and selectivity.
Choose EZH2 or other HMT inhibitors for studying PRC2-dependent gene silencing
EZH2, the catalytic subunit of polycomb repressive complex 2 (PRC2), catalyzes H3K27 trimethylation (H3K27me3), a histone modification associated with repression of tumor suppressor genes, and EZH2 inhibitors like tazemetostat are used in research to probe the role of this silencing mark.
Combine inhibitors to study epistatic relationships between epigenetic marks
Because DNA methylation, histone acetylation, and histone methylation are mechanistically linked, combining HDAC inhibitors with DNMT inhibitors can produce synergistic effects on gene expression exceeding either alone, and these combination approaches are both experimental tools and the basis for combination epigenetic therapy research.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
HDAC and DNMT inhibitors are the most clinically advanced classes of epigenetic therapeutics, with multiple HDAC inhibitors including vorinostat and romidepsin approved for hematological malignancies, EZH2 inhibitors including tazemetostat having received accelerated approval, and multiple BET inhibitors in clinical trials. As of 2026, CRISPR/dCas9 systems fused with DNMTs, TETs, and HDACs enable locus-specific epigenome editing, extending classic enzyme and inhibitor approaches toward targeted epigenetic modulation.
<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP</a> and find the suitable enzymes and inhibitory reagents for your lab.

Enzymes & Inhibitors

DataSheet

MSDS

Protocol

DNA Degradase

The DNA Degradase by Zymo Research is a nuclease mixture at 10 U/µL. Degrades DNA to individual nucleotide components in ~1 hour at 37°C. One unit degrades 1 µg of lambda DNA. Used for whole-genome methylation analysis by LC/MS, HPLC, and TLC. Store at −20°C. Available in 500 U and 2000 U sizes. Available in 500 U (E2016) and 2000 U (E2017).

Assay Condition

Concentration

Enzyme Inactivation

Storage

Unit Definition

Manufacturer

Shipping Condition

Storage Condition

DNA Degradase™ (500 U)

DNA Degradase (2000 U)

DNA Degradase Plus

The DNA Degradase Plus&trade; by Zymo Research is a 5 U/&micro;L DNA Degradase Plus enzyme mixture (250 units) that digests double-stranded DNA to individual nucleosides in approximately 2 hours at 37&deg;C, enabling quantitative LC/MS analysis of DNA methylation marks including 5mC and 5hmC. Available in 250 U (E2020) and 1,000 U (E2021).

DNA Degradase Plus™ (250 U)

DNA Degradase Plus™ (1000 U)

<h2>What are bisulfite conversion reagents and buffers?</h2>
&nbsp;
Bisulfite conversion reagents include sodium bisulfite solution, denaturation and conversion buffers, and complete kits that bundle all components needed for the three-step chemical reaction: sulfonation of cytosine to a cytosine-bisulfite derivative, hydrolytic deamination to a uracil-bisulfite derivative, and alkali desulfonation to yield uracil. This process converts unmethylated cytosines to uracil while leaving 5-methylcytosine intact and unconverted, because 5-methylcytosine resists bisulfite-mediated deamination under standard conditions.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">RNA and DNA conversion reagents for enhanced modification of cytosine in methylation workflows</li>
<li dir="ltr" role="presentation">Desulphonation buffers for stabilization of nucleic acids and post-conversion cleanup&nbsp;</li>
<li dir="ltr" role="presentation">Binding buffers used during purification for efficient capture of converted RNA and DNA</li>
<li dir="ltr" role="presentation">Wash buffers for the removal of inhibitors and improved purity of samples</li>
<li dir="ltr" role="presentation">Elution buffers for high-quality recovery of converted nucleic acids</li>
<li dir="ltr" role="presentation">DNA denaturing and digestion buffers for controlled workflow and optimized conversion efficiency</li>
<li dir="ltr" role="presentation">Dilution and reaction buffers for maintaining stable assay conditions</li>
<li dir="ltr" role="presentation">Solubilization and dissolution buffers for sample processing and preparation&nbsp;</li>
<li dir="ltr" role="presentation">Lightning conversion reagents for efficient and rapid methylation analysis workflows</li>
</ul>
&nbsp;
<h2>How to use bisulfite conversion reagents</h2>
&nbsp;
Ensure complete DNA denaturation before conversion to avoid incomplete conversion
Bisulfite only acts on cytosines in single-stranded DNA, so complete denaturation of the input DNA is the most critical first step, since failure to fully denature can leave double-stranded regions in which cytosines are shielded from the bisulfite reagent and remain unconverted.
Understand that incomplete conversion produces false-positive methylation results
If the conversion is incomplete, unconverted unmethylated cytosines will read as cytosine after sequencing, which will be incorrectly interpreted as methylated cytosine, artificially inflating the apparent methylation level of the sample in a false-positive direction.
Optimize conversion conditions to balance completeness against DNA degradation
The harsh conditions needed for complete bisulfite conversion, including high bisulfite concentration, elevated temperature, and long incubation, also fragment and degrade DNA, and optimizing conditions that achieve high conversion while minimizing degradation is a key technical challenge in bisulfite workflows.
Include an unmethylated conversion control to estimate residual incomplete conversion
Including an unmethylated control DNA, such as lambda phage DNA, in the same conversion reaction provides a readout of how completely unmethylated cytosines were converted, helping quantify residual incomplete conversion rates and account for them in downstream analysis.
Be aware of specific challenges for FFPE and degraded DNA samples
Formalin-fixed, paraffin-embedded tissue DNA is already fragmented before bisulfite treatment, and the additional fragmentation during conversion can result in poor library yields; specific modified protocols and carrier reagents can help reduce further degradation during the conversion step.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Bisulfite sequencing is widely regarded as the gold standard for DNA methylation analysis due to its high resolution and accuracy at single-base level, but it also introduces specific DNA quality challenges for downstream library preparation and sequencing, particularly for samples already of poor quality like FFPE tissue. As of 2026, enzymatic conversion is increasingly recommended as the preferred alternative to bisulfite conversion specifically because it achieves comparable discrimination between methylated and unmethylated cytosines while minimizing the DNA damage and library complexity loss that harsh bisulfite chemistry introduces.
This category sits within DNA/RNA Methylation under Epigenetics, and for a synthetic, fully methylated positive control to run through the conversion workflow, see dNTPs (Deoxynucleotide Triphosphates). See the full reagents catalog and use Quick Order for recurring orders.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP and request a quote today to get high-quality bisulfite conversion reagents for your lab.

Bisulfite Conversion Reagents & Buffers

DNA Denaturing Buffer (1 ml)

DNA Denaturing Buffer (D5101-4-1) by Zymo Research — 1 ml buffer for DNA strand denaturation in the Methylated-DNA IP Kit (D5101) MeDIP workflow. Stable from 0°C to room temperature. Sold separately or as part of the D5101 kit. Made in the USA.

<h2>What Are Zymo Research Buffers?</h2>
Zymo Research buffers are formulation-specific reagents used at defined steps &mdash; lysis, binding, wash, and elution &mdash; in Zymo-Spin column-based purification protocols for DNA, RNA, and bisulfite-converted DNA. Each buffer is optimized for a specific kit family: DNA Wash Buffer concentrate (requires ethanol addition before use) removes salts and contaminants before elution from Zymo-Spin silica columns; DNA Elution Buffer (10 mM Tris, pH 8.5, 0.1 mM EDTA) releases purified DNA from the matrix; RNA Wash Buffer supports RNA extraction kits including Quick-RNA and Direct-zol lines; M-Elution Buffer is designed for eluting bisulfite-converted DNA in EZ DNA Methylation kits. Researchers typically order these separately when a single buffer component runs out before the rest of the kit, or when scaling up a protocol beyond the volume included.
TL;DR: Order Zymo buffers individually to replenish a specific step in your existing Zymo workflow without replacing the full kit.
<h2>How to Choose Zymo Research Buffers</h2>
<h3>Match the buffer to your kit family</h3>
Every Zymo buffer is paired to a specific kit line. DNA Wash Buffer and DNA/RNA Wash Buffer concentrate are used in DNA Clean &amp; Concentrator, Quick-DNA, and Zymoclean Gel DNA Recovery kits. RNA Wash Buffer pairs with Quick-RNA kits. ZymoBIOMICS DNA Wash Buffer 1 and Wash Buffer 2 are specific to the ZymoBIOMICS microbiomics line. Substituting a buffer from a different kit family can compromise yield or purity.
<h3>Confirm ethanol addition requirements before use</h3>
Several Zymo wash buffer concentrates require addition of 96-100% ethanol to the bottle before first use &mdash; the label states the exact volume. DNA Wash Buffer concentrate, DNA/RNA Wash Buffer concentrate, and Urine DNA Wash Buffer concentrate all require this step. Pre-made (non-concentrate) versions such as RNA Wash Buffer are supplied ready to use.
<h3>Select the right volume for your throughput</h3>
Zymo buffers are available in sizes from 6 ml (single-experiment replenishment) up to 500 ml (Buffer P1 Red, for high-volume plasmid prep labs). For labs processing more than 50 samples per week, ordering the largest available size reduces per-sample cost and the frequency of reordering.
<h3>Choose elution buffer chemistry based on downstream application</h3>
DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA) is the standard for most PCR and sequencing applications. For applications sensitive to EDTA &mdash; such as some restriction enzyme digests or direct use with divalent cation-dependent enzymes &mdash; eluting in molecular-grade water or a Tris-only buffer is preferable. M-Elution Buffer is specifically formulated for bisulfite-converted DNA and should not be substituted with standard elution buffer in methylation workflows.
<h3>Verify research-use-only status</h3>
All Zymo Research buffers sold through MBP are for research use only (RUO). They are not cleared for in vitro diagnostic use. For clinical or diagnostic laboratory applications, confirm regulatory status with your institutional compliance officer before use.
<h2>Compare Zymo Buffer Types</h2>
<table>
<thead>
<tr>
<th>Buffer Type</th>
<th>Primary Application</th>
<th>Kit Family</th>
<th>Format Available</th>
</tr>
</thead>
<tbody>
<tr>
<td>DNA Wash Buffer (concentrate)</td>
<td>Salt removal before DNA elution</td>
<td>DNA Clean &amp; Concentrator, Quick-DNA</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA/RNA Wash Buffer (concentrate)</td>
<td>Salt removal for dual DNA/RNA preps</td>
<td>Quick-DNA/RNA kits</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA)</td>
<td>DNA release from silica column</td>
<td>All Zymo DNA purification</td>
<td>16 ml &ndash; available sizes vary</td>
</tr>
<tr>
<td>RNA Wash Buffer</td>
<td>RNA purification wash step</td>
<td>Quick-RNA kits</td>
<td>Ready-to-use</td>
</tr>
<tr>
<td>M-Elution Buffer</td>
<td>Elution of bisulfite-converted DNA</td>
<td>EZ DNA Methylation kit families</td>
<td>Small volume</td>
</tr>
<tr>
<td>ZymoBIOMICS DNA Wash Buffer 2</td>
<td>Metagenomic/microbiomic DNA cleanup</td>
<td>ZymoBIOMICS DNA kits</td>
<td>Sold separately</td>
</tr>
<tr>
<td>Buffer P1 Red</td>
<td>Alkaline lysis resuspension (plasmid prep)</td>
<td>Zyppy Plasmid Miniprep</td>
<td>10 ml &ndash; 500 ml</td>
</tr>
</tbody>
</table>
<h2>Specifications Context</h2>
Zymo wash buffer concentrates are supplied at a defined fold-concentration (commonly 4x or 6x) and require ethanol addition to reach working strength &mdash; the exact ratio is printed on the bottle and in the kit protocol. Elution buffer volumes for Zymo-Spin columns typically range from 6 to 100 &micro;l per elution; larger elution volumes improve total yield at the cost of DNA concentration. For DNA elution from Zymo-Spin columns, the protocol recommends a minimum 1-minute contact time between elution buffer and the silica matrix to maximize recovery of fragments 10 kb and above. As of 2025, Zymo Research tests all buffer lots for performance consistency, with integrity guaranteed for up to one year from purchase date.
Browse the full <a href="https://www.notion.so/reagents">Zymo Research reagents collection at MBP</a> for kits and columns that use these buffers, or visit our <a href="https://www.notion.so/reagents/buffers">reagent buffers category</a> for non-Zymo buffer options including ABM and other brands. For volume orders or purchase orders, use the <a href="https://www.notion.so/quickorder">Quick Order</a> tool or <a href="https://www.notion.so/contact-us">contact us</a> directly &mdash; MBP is a registered vendor for institutional procurement systems.
Last updated: June 2025
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Zymo Buffers

DNA Digestion Buffer

DNA Digestion Buffer is available in the following variants: E1010-1-16, E1010-1-4.E1010-1-16 by Zymo Research is the DNA Digestion Buffer (16 mL), the 1x reaction buffer for DNase I (Zymo Research E1010 DNase I Set). Used for in-solution DNA digestion to prepare DNA-free RNA for RT-PCR, post-IVT template removal, and pre-sequencing cleanup. Inactivate after use by adding 5 mM EDTA and heating to 75°C for 10 minutes.

DNA Digestion Buffer (16 ml)

DNA Digestion Buffer (4 ml)

DNA Elution Buffer

Zymo Research DNA Elution Buffer: 10 mM Tris pH 8.5, 0.1 mM EDTA, ready-to-use. For silica columns, 96-well plates, and magnetic beads. Five volumes: D3004-4-1 (1 mL), D3004-4-4 (4 mL), D3004-4-10 (10 mL), D3004-4-16 (16 mL), D3004-4-50 (50 mL).

DNA Elution Buffer (1 ml)

DNA Elution Buffer (10 ml)

DNA Elution Buffer (16 ml)

DNA Elution Buffer (4 ml)

DNA Elution Buffer (50 ml)

DNA Pre-Wash Buffer

Zymo Research DNA Pre-Wash Buffer: intermediate protein-removal wash for Quick-DNA genomic DNA purification kits (D3024, D3020, D3067 and related). Four volumes: D3004-5-15 (15 mL), D3004-5-30 (30 mL), D3004-5-50 (50 mL), D3004-5-250 (250 mL).

DNA Pre-Wash Buffer (15 ml)

DNA Pre-Wash Buffer (250 ml)

DNA Pre-Wash Buffer (30 ml)

DNA Pre-Wash Buffer (50 ml)

DNA Recovery Buffer (9 ml)

DNA Recovery Buffer R2050-5-9 by Zymo Research — 9 ml buffer for use in the Quick-DNA/RNA Blood Tube Kit (Cat. R1151), removing contaminants from DNA and RNA extracted from whole blood preserved with DNA/RNA Shield™. Sold separately for buffer component replenishment. Order via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or contact <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.

DNA/RNA Buffer HT (100 ml)

R2150-1-100 by Zymo Research is the DNA/RNA Buffer HT (100 ml), a one-step lysis and binding reagent for high-throughput simultaneous DNA and RNA extraction using the Quick-DNA/RNA HT magnetic bead system. Compatible with 96-well and 24-well formats; eluates ready for NGS and RT-qPCR. Requires user addition of beta-mercaptoethanol before use.

<h2>What Are Nucleases?</h2>
&nbsp;
Nucleases are enzymes that cleave phosphodiester bonds between nucleotide subunits in nucleic acids. Deoxyribonucleases (DNases) act on DNA; ribonucleases (RNases) act on RNA; and some nucleases (S1 Nuclease, Mung Bean Nuclease) act on both single-stranded DNA and RNA. Nucleases are further divided into endonucleases, which cleave internal phosphodiester bonds, and exonucleases, which progressively remove nucleotides from the 5' or 3' terminus of a strand. Major research nucleases include DNase I (non-specific endonuclease for both ss- and ds-DNA), RNase A (cleaves single-stranded RNA at pyrimidine residues), RNase H (degrades the RNA strand of RNA: DNA hybrids), Exonuclease III (removes nucleotides from 3'-recessed or blunt ends of duplex DNA), S1 Nuclease (degrades single-stranded DNA or RNA), and RNase R (degrades linear RNA but not circular RNA). Match the nuclease to the substrate type (single-stranded vs. double-stranded, DNA vs. RNA), the directionality required (endo vs. exo), and cofactor compatibility with your reaction buffer.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
PureRec RNase A (Recombinant): A ribonuclease free of animal-derived components, designed for the swift elimination of RNA from DNA samples, ensuring no threat of viral contamination.
</li>
<li dir="ltr" aria-level="1">
DNase I Set (RNase-free): A highly effective endonuclease formulated for complete DNA digestion in delicate RNA applications while preserving the integrity of the transcript.
</li>
<li dir="ltr" aria-level="1">
PureRec Duplex-Specific Nuclease (DSN): A unique enzyme designed to equalize high-abundance transcripts, greatly enhancing the identification of rare variants in cDNA libraries.
</li>
<li dir="ltr" aria-level="1">
RNase R: A distinctive 3'-to-5' exoribonuclease that breaks down linear RNA but preserves circular RNA forms, crucial for the identification of circRNA.
</li>
<li dir="ltr" aria-level="1">
RNaseOFF Inhibitor: A strong protein-based barrier that counteracts a wide range of RNases, maintaining the delicate transcriptional environment of your samples.
</li>
</ul>
&nbsp;
<h2>How to Choose Nucleases</h2>
&nbsp;
DNase selection
DNase I (bovine pancreatic, recombinant) non-specifically cleaves both single- and double-stranded DNA in the presence of Mg2+ (random cleavage) or Mn2+ (double-strand cuts at approximately the same position on both strands). Use RNase-free DNase I for RNA preparations to remove genomic DNA contamination. Use Plasmid-Safe ATP-Dependent DNase to selectively degrade linear double-stranded DNA while preserving supercoiled and relaxed circular plasmid DNA. Baseline-ZERO DNase is optimized for the complete removal of trace DNA without damaging RNA.
RNase selection
RNase A cleaves single-stranded RNA at pyrimidine (C and U) residues to produce 3'-pyrimidine nucleotides; it is used to remove RNA from DNA preps, to perform RNase protection assays, and to analyze RNA secondary structure. RNase H degrades the RNA strand of RNA: DNA duplexes and is used after first-strand cDNA synthesis to remove the mRNA template. RNase R (3'-&gt;5' exoribonuclease) degrades all linear RNA without affecting circular RNA, and is used in circular RNA enrichment workflows and to characterize circular RNA splice junctions. Terminator 5'-Phosphate-Dependent Exonuclease (Terminator) digests linear RNA with a 5'-monophosphate, enriching for capped mRNA or circular RNA.
Exonuclease selection
Exonuclease III (Exo III) removes nucleotides from the 3' terminus of duplex DNA and generates single-stranded regions used in unidirectional nested deletions, mapping regulatory elements, and generating single-stranded templates for sequencing. Exonuclease I (Exo I) digests single-stranded DNA in the 3'-&gt;5' direction and is used after PCR to remove unincorporated primers before Sanger sequencing. Lambda Exonuclease removes nucleotides from 5'-phosphorylated duplex DNA in the 5'-&gt;3' direction and is used in isothermal amplification and strand displacement workflows.
Cofactor and inhibitor sensitivity
DNase I requires Mg2+ or Ca2+ for activity and is completely inhibited by EDTA at a 5 mM concentration. RNase A is active across a wide pH range (optimum 7.0-7.5) and is exceptionally stable; inactivation requires proteinase K digestion, phenol-chloroform extraction, or RNase inhibitor protein. RNase H requires Mg2+; RNase R requires Mg2+ and is most active between 37-50 degrees C.
Purity and application grade
For RNA-focused applications, all nucleases used on DNA substrates must be certified RNase-free. For DNA-focused work, DNase-free grades of RNases are required. Confirm lot-specific QC contamination data from the manufacturer before use in sensitive workflows.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
DNase I activity is expressed in Kunitz units: 1 unit increases A260 by 0.001 per minute per mL during DNA hydrolysis at pH 5.0. RNase A activity is expressed as the amount of enzyme hydrolyzing 50% of an RNA substrate under defined conditions; it remains active at a wide range of temperatures and is notoriously difficult to inactivate (requires boiling in SDS, proteinase K digestion, or phenol-chloroform extraction). RNase R from E. coli degrades linear RNA processively in the 3'-&gt;5' direction and is used at 2-10 U per microgram of total RNA to enrich for circular RNA species. RNase R treatment before library construction is the standard method for circular RNA sequencing (circRNA-seq) workflows on both short-read and long-read platforms. For protecting RNA from RNase contamination during nuclease-adjacent workflows, see&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/enzymes-and-inhibitors">RNase inhibitors</a>&nbsp;in the parent Enzymes and Inhibitors category.
&nbsp;
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Nucleases

DNase I Set (RNase-free)

The DNase I Set (RNase-free) by Zymo Research is a recombinant DNase I Set (RNase-free), supplied lyophilized with DNA Digestion Buffer. One unit = 12 Kunitz units. Guaranteed RNase-free and protease-free; stable through ≥10 freeze-thaw cycles. Heat-inactivated at 75°C/10 min. Available in 250 U, 1500 U, and 5×1500 U. Available in 250 U (E1010), 1500 U (E1011), and 5 x 1500 U (E1012).

Reconstitution

Inactivation

DNase I Set (250 U) w/ DNA Digestion Buffer (4 ml)

DNase I Set (1500 U) w/ DNA Digestion Buffer (4 ml)

DNase I Set (5 x 1500 U) w/ DNA Digestion Buffer (16 ml)

Tubes

DNase/RNase-Free Tubes (1.5 ml)

The DNase/RNase-Free Tubes (1.5 mL) by Zymo Research are polypropylene microcentrifuge tubes certified free of DNase and RNase contamination, suitable for RNA isolation, DNA extraction, and molecular biology sample storage. Manufactured in the USA. Available as C2001-50 (Pack of 50) and C2001-100 (Pack of 100).

DNase/RNase-Free Tubes (1.5 ml) (100 Tubes)

DNase/RNase-Free Tubes (1.5 ml) (50 Tubes)

<h2 dir="ltr"> What Are Miscellaneous PCR Items?</h2>
 Miscellaneous Items groups supporting reagents used alongside core PCR enzymes, master mixes, and nucleotide components, covering essential but non-core materials required for PCR and RT-PCR workflows. This includes nuclease-free water for reaction setup and dilution, Random Primer (6N) and Oligo(dT)18 primers for cDNA synthesis, and ionic solutions such as MgCl₂, MgSO₄, NaCl, and MnCl₂ used for reaction optimisation. The category also includes certified nuclease-free water options from suppliers such as BIOLOGIX for sensitive molecular biology applications. Use this category to source essential supporting reagents that complete PCR-based experimental workflows alongside primary amplification products. 
&nbsp;
<h2 data-start="432" data-end="459">What you will find:</h2>
&nbsp;
<ul data-start="461" data-end="1220">
<li data-section-id="b5abnd" data-start="461" data-end="635">Nuclease-Free Water: DNase/RNase-free water suitable for PCR, RT-PCR, qPCR, and nucleic acid preparation workflows, including certified molecular biology-grade options.</li>
<li data-section-id="n5mfl0" data-start="636" data-end="789">Primers &amp; Oligos: Random Primer (6N) and Oligo(dT)18 formats for reverse transcription, cDNA synthesis, and related molecular biology applications.</li>
<li data-section-id="1x29qzm" data-start="790" data-end="932">Reaction Components: Molecular biology-grade MgCl₂, MgSO₄, MnCl₂, and NaCl solutions for reaction preparation and protocol optimisation.</li>
<li data-section-id="16hzrat" data-start="933" data-end="1075">Workflow Support Reagents: Essential supporting materials used alongside PCR enzymes, master mixes, and nucleic acid amplification kits.</li>
<li data-section-id="4t6tsy" data-start="1076" data-end="1220">Research-Ready Formats: Convenient reagent sizes suitable for routine academic, biotechnology, and molecular biology laboratory workflows.</li>
</ul>
<h2 dir="ltr"> How to Choose Miscellaneous PCR Items</h2>
 Gel Loading Dyes Loading dyes add density and colour to PCR products for gel electrophoresis, allowing visual tracking of sample migration. Some 2x master mixes include loading dye pre-formulated, but a separate loading dye is needed when using dye-free master mixes or individual-component PCR setups. PCR Enhancers and Additives Additives such as betaine, DMSO, or proprietary enhancer formulations can improve amplification of GC-rich or otherwise difficult templates by reducing secondary structure or improving primer annealing specificity -- useful as a troubleshooting tool when a standard reaction underperforms on a specific target. Nuclease-Free Water Grade Nuclease-free water is used for reaction dilutions, resuspending primers and probes, and as a no-template control in PCR and qPCR setups. Confirm the water is certified free of detectable RNase and DNase activity, particularly for RNA-handling workflows. PCR Tube and Plate Format PCR tubes, strips, and plates are available in formats matching common thermal cycler block configurations -- 0.2 mL individual tubes, 8-well or 12-well strips, and 96-well plates -- select the format matching your thermal cycler and throughput needs. Compatibility with Primary Reagents When ordering supporting consumables alongside primary PCR reagents, confirming tube/plate compatibility with both your thermal cycler and any downstream automation (liquid handlers, plate readers) avoids workflow bottlenecks from mismatched formats. 
<h2 dir="ltr"> Specifications Context</h2>
&nbsp;
Nuclease-free water used in molecular biology workflows is typically certified through DNase/RNase activity testing and supplied in formats suitable for routine PCR, RT-PCR, and nucleic acid dilution applications. Random Primer (6N) and Oligo(dT)18 primers are used in reverse transcription workflows to support cDNA synthesis from RNA templates. Ionic solutions such as MgCl₂, MgSO₄, NaCl, and MnCl₂ are used to support and optimise polymerase activity under different reaction conditions. Most items in this category are stable under standard laboratory storage conditions, with product-specific handling and storage requirements provided by the manufacturer.
&nbsp;
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Miscellaneous Items

DNase/RNase-Free Water

W1001 by Zymo Research is DNase/RNase-free water for use in all DNA, RNA, and molecular biology applications. Available in 1, 4, 6, 10, and 30 ml formats (W1001-1 through W1001-30), manufactured in the USA with certificate of analysis. Lab managers and academic researchers running RNA, NGS, or qPCR workflows rely on W1001 as a traceable, nuclease-free aqueous reagent from a trusted supplier.

DNase/RNase-free Water (1 ml)

DNase/RNase-free Water (10 ml)

DNase/RNase-free Water (30 ml)

DNase/RNase-free Water (4 ml)

DNase/RNase-free Water (6 ml)

<h2 dir="ltr"> What is a dNTP Mix?</h2>
 A dNTP Mix is a premixed solution containing all four deoxynucleotide triphosphates - dATP, dCTP, dGTP, and dTTP - combined in equimolar amounts at a standard working concentration, commonly 10 mM each (40 mM total) when prepared from 100 mM individual stocks. The premix is ready for direct addition to a PCR, qPCR, or cDNA synthesis reaction without further dilution or balancing.&nbsp; Choose a premixed dNTP Mix for routine PCR setup where convenience and lot-to-lot consistency outweigh the need for custom nucleotide ratios.
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<h2 dir="ltr">What you will find:</h2>
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<li>High-purity nucleotide mixes for PCR and DNA synthesis applications, including PureStrand&trade; Ultra-Pure dNTP Mix (Azura)</li>
<li>Standard 10 mM each dNTP formulations suitable for routine PCR workflows, including dNTP Mix (10 mM each) formats</li>
<li>Higher-concentration nucleotide solutions, such as Accuris 40 mM dNTP Mix for optimized reaction setup flexibility</li>
<li>Ready-to-use balanced dNTP blends supporting DNA polymerase activity in PCR and related enzymatic amplification reactions</li>
<li>Nucleotide triphosphate formulations, including NTP Mix (G945), for broader enzymatic and molecular biology applications</li>
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<h2>How to Choose a dNTP Mix</h2>
 Concentration Format Common premix formats include 10 mM each (40 mM total, a 50x working stock relative to the 0.2 mM final concentration) and 2.5 mM each (10 mM total, a 10x working stock). Select the format that aligns with your typical reaction volume and pipetting precision - more dilute stocks reduce pipetting error for small-volume reactions. Equimolar Accuracy Because all four nucleotides are pre-combined at equal concentrations, a premixed dNTP Mix removes the risk of manual equimolar-mixing error that can occur when combining four separate 100 mM stocks - unbalanced ratios increase misincorporation frequency during polymerisation. Throughput Considerations For high-throughput PCR setups across multi-well plates, a premixed dNTP Mix reduces the number of individual pipetting steps per reaction compared to adding four separate dNTP stocks, improving both speed and reproducibility. Compatibility with Master Mixes Many 2x PCR master mixes already include dNTPs at optimised concentrations - a separate dNTP Mix is primarily needed for labs assembling reactions from individual components (enzyme, buffer, dNTPs, primers) rather than using a pre-formulated master mix. Application Range A standard equimolar dNTP Mix supports PCR, qPCR, and cDNA synthesis (as substrate for reverse transcriptase). Confirm the concentration and purity grade align with the specific requirements of high-fidelity or long-range PCR, if applicable. 
<h2 dir="ltr"> Specifications Context</h2>
 To prepare a standard PCR reaction at the recommended 0.2 mM final concentration of each dNTP using a 10 mM-each premix, 1 &micro;L of the premix is added per 50 &micro;L reaction. Equal nucleotide concentrations in the premix are essential because unbalanced dNTP ratios increase the frequency of misincorporation during DNA synthesis - a factory-balanced premix removes this variable. dNTP Mix is typically supplied in water at pH 7.5, stable at &minus;20 &deg;C with documented multiple freeze-thaw tolerance; thawing and mixing thoroughly before use is recommended, especially for higher-concentration formats. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get high-purity dNTP mixes tailored for your laboratory workflows.

dNTPs Mix

dNTP Mix (10 mM)

The dNTP Mix (10 mM) by MBP is a ready-to-use equimolar solution of dATP, dCTP, dGTP, and dTTP at 10 mM each, formulated for PCR, RT-PCR, and NGS library preparation. Available as D1000-1 (100 µl) for low-throughput use and D1000 for higher-volume applications — store at -20°C in a non-frost-cycle freezer.