Zymo Research

<h2>What are epigenetics enzymes and inhibitors?</h2>
&nbsp;
Epigenetics enzymes and inhibitors are the molecular tools for directly studying or modulating the enzymatic machinery that writes, erases, or reads epigenetic marks. The major classes include: DNA methyltransferases (DNMT1, DNMT3A, DNMT3B), which catalyze the addition of a methyl group to cytosine; TET family enzymes (TET1, TET2, TET3), which oxidize 5-methylcytosine to 5-hydroxymethylcytosine; histone acetyltransferases (HATs) and histone deacetylases (HDACs), which add and remove acetyl groups from histone lysine residues; and histone methyltransferases (HMTs) including EZH2, DOT1L, and G9a. Small-molecule inhibitors of HDAC and DNMT enzymes are the most clinically and experimentally advanced of these tools.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Micrococcal nuclease for nucleosome mapping and controlled chromatin digestion applications</li>
<li dir="ltr" role="presentation">DNA shearing enzymes for consistent fragmentation of DNA in sequencing and epigenetic workflows</li>
<li dir="ltr" role="presentation">DNA degradation enzymes for controlled breakdown and analysis of nucleic acids&nbsp;</li>
<li dir="ltr" role="presentation">CpG methylase enzymes for targeted DNA methylation (CpG-specific )&nbsp; in epigenetic modification studies</li>
<li dir="ltr" role="presentation">5-hmC glucosyltransferase for detection and labeling of hydroxymethylcytosine in DNA</li>
<li dir="ltr" role="presentation">SAM (S-adenosylmethionine) cofactors for supporting enzyme activity and methylation reactions</li>
<li dir="ltr" role="presentation">DNA digestion enzymes for genomic DNA processing workflows</li>
<li dir="ltr" role="presentation">dsDNA nuclease systems for controlled cleavage and analysis of double-stranded DNA</li>
</ul>
&nbsp;
<h2>How to choose epigenetics enzymes and inhibitors</h2>
&nbsp;
Choose DNMT inhibitors to globally reduce or deplete DNA methylation in cells
Nucleoside analog DNMT inhibitors like 5-azacytidine and decitabine incorporate into DNA and trap DNMT1, preventing maintenance methylation and causing passive demethylation through DNA replication, while newer non-nucleoside DNMT inhibitors are in development for more selective, non-genotoxic methylation reduction.
Choose HDAC inhibitors to globally increase histone acetylation
HDAC inhibitors including pan-HDAC hydroxamates like vorinostat (SAHA) or class-selective compounds reduce histone deacetylase activity and cause hyperacetylation of histone lysine residues, broadly promoting gene expression at silenced loci. HDAC inhibitors are the most clinically advanced class of epigenetic therapeutics, with multiple FDA-approved compounds.
Use TET enzyme preparations for in vitro 5-hmC oxidation studies
Purified TET1, TET2, or TET3 enzyme preparations allow controlled in vitro oxidation of 5-methylcytosine to 5-hydroxymethylcytosine and further oxidation products, useful for generating defined 5-hmC-containing substrate DNA or studying TET enzyme activity and selectivity.
Choose EZH2 or other HMT inhibitors for studying PRC2-dependent gene silencing
EZH2, the catalytic subunit of polycomb repressive complex 2 (PRC2), catalyzes H3K27 trimethylation (H3K27me3), a histone modification associated with repression of tumor suppressor genes, and EZH2 inhibitors like tazemetostat are used in research to probe the role of this silencing mark.
Combine inhibitors to study epistatic relationships between epigenetic marks
Because DNA methylation, histone acetylation, and histone methylation are mechanistically linked, combining HDAC inhibitors with DNMT inhibitors can produce synergistic effects on gene expression exceeding either alone, and these combination approaches are both experimental tools and the basis for combination epigenetic therapy research.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
HDAC and DNMT inhibitors are the most clinically advanced classes of epigenetic therapeutics, with multiple HDAC inhibitors including vorinostat and romidepsin approved for hematological malignancies, EZH2 inhibitors including tazemetostat having received accelerated approval, and multiple BET inhibitors in clinical trials. As of 2026, CRISPR/dCas9 systems fused with DNMTs, TETs, and HDACs enable locus-specific epigenome editing, extending classic enzyme and inhibitor approaches toward targeted epigenetic modulation.
<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP</a> and find the suitable enzymes and inhibitory reagents for your lab.

Enzymes & Inhibitors

MSDS

dsDNA Shearase

dsDNA Shearase is available in the following variants: E2019-200, E2019-50.dsDNA Shearase (E2019-200) by Zymo Research — dsDNA Shearase Plus endonuclease (1 U/µL, 200 U) bundled with DNA Clean &amp; Concentrator-5 (DCC-5). Fragments 250 ng human DNA to 100–500 bp at 42°C in 20 min; elute purified fragments in as little as 10 µL. Ready for NGS and MeDIP workflows.

Concentration

Enzyme Inactivation

Storage

Unit Definition

Manufacturer

Shipping Condition

Storage Condition

dsDNA Shearase™ (200 U)

dsDNA Shearase™ Plus (50 U)

DataSheet

Protocol

dsDNA Shearase Plus

dsDNA Shearase Plus is available in the following variants: E2018-200, E2018-50.dsDNA Shearase Plus (E2018-200) by Zymo Research — 1 U/µL, 200 U. Fragments 250 ng human DNA to 100–500 bp in 20 min at 42°C in 10 µL. Generates 5′-phosphate/3′-OH termini ready for NGS adapter ligation. Enzyme only; for the DCC-5 bundle see E2019-200.

dsDNA Shearase™ Plus (200 U)

<h2>What are epigenetics dNTPs?</h2>
&nbsp;
Epigenetics dNTPs includes both standard deoxynucleotide triphosphate sets used across many molecular biology applications and modified nucleotide analogs relevant specifically to epigenetic research, with the most important being 5-methyl-dCTP, a base-modified analog in which the cytosine base carries a methyl group at the 5-carbon position. 5-methyl-dCTP can be incorporated into DNA via PCR or in vitro synthesis in place of standard dCTP, producing a defined, fully methylated cytosine-substituted DNA molecule useful as a positive control for methylation detection assays or as a substrate for studying methylation-related enzyme activity.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li><a href="https://mbpinc.net/reagents/epigenetics/dntps-deoxynucleotide-triphosphates/methylated">Methylated: </a>dNTP mixes used in methylation-sensitive DNA synthesis workflows.&nbsp;Methylated nucleotide analogs for DNA methylation studies and epigenetics research.&nbsp;Standard individual dNTPs (dATP, dCTP, dGTP, dTTP) for PCR analysis and routine DNA synthesis.</li>
</ul>
&nbsp;
<h2>How to choose epigenetics dNTPs</h2>
&nbsp;
Use 5-methyl-dCTP to build defined, fully methylated control DNA
Substituting 5-methyl-dCTP for standard dCTP in a PCR reaction produces a fully methylated cytosine-substituted amplicon, providing a positive control whose methylation status is defined and known, unlike a positive control derived from genomic DNA with variable, unknown methylation patterns.
Confirm that your methylation detection assay is validated with a defined methylated control
Running a fully methylated positive control through a bisulfite conversion or enzymatic conversion workflow confirms the detection method is correctly interpreting methylated cytosines, helping catch conversion efficiency problems before they affect interpretation of real sample results.
Use methylated dNTP-synthesized DNA to study methylation-sensitive restriction digestion
Some restriction enzymes specifically cleave methylated sequences while others are inhibited by methylation, and a fully methylated DNA produced with 5-methyl-dCTP is a useful substrate for confirming whether a specific enzyme's activity is methylation-sensitive.
Be aware that 5-methyl-dCTP is also believed to be the starting substrate for TET enzyme oxidation
5-methylcytosine is believed to be the substrate for TET family enzymes that produce 5-hydroxymethylcytosine and further oxidation products, making 5-methyl-dCTP-synthesized DNA a potential substrate for in vitro TET enzyme activity studies.
Confirm nucleotide concentration and compatibility with your synthesis conditions
Standard dNTPs and modified analogs like 5-methyl-dCTP are typically supplied as concentrated solutions requiring dilution, and confirming appropriate concentration and buffer compatibility before use ensures consistent, reproducible incorporation into your target DNA.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
5-methyl-dCTP is typically supplied as a 10 mM solution and offers the ability to enzymatically make a defined, fully methylated, cytosine-substituted DNA by PCR or in vitro synthesis, a property that makes it a valuable resource for a variety of biochemical and cellular applications in epigenetics research. As of 2026, methylated DNA probes made with 5-methyl-dCTP continue to serve as methylation reference fragments and as pull-down substrates for 5-hydroxymethylcytosine-binding proteins from cellular lysates, extending their use beyond simple positive controls.
<a href="https://mbpinc.net/contact-us">Contact the expert team at Molecular Biology Products Inc. (MBP)</a> to find high-integrity dNTPs tailor-made for your research applications.

dNTPs (Deoxynucleotide Triphosphates)

dTTP (100 mM) (250 µl)

D1010 by MBP is a 100 mM dTTP (deoxythymidine triphosphate) solution supplied in a 250 µl volume, formulated for direct use in PCR, RT-PCR, nucleotide mix preparation, and library construction protocols. Store at -20°C.

<h2 data-section-id="b4wzzk" data-start="0" data-end="47">What is bacterial culture media for cloning?</h2>
&nbsp;
Bacterial culture media for cloning workflows includes LB (Luria-Bertani) broth and agar, the standard general-purpose media for E. coli growth, SOC media used for post-transformation recovery, and Terrific Broth for higher-density culture in plasmid preparation. Pre-made agar plates with antibiotic selection (ampicillin, kanamycin, chloramphenicol, and others matching common selection markers) are used to isolate colonies carrying the desired plasmid. Related entities include antibiotic selection markers matching transformation kit resistance genes, post-transformation recovery time, and plasmid yield scaling with culture volume and media richness.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">ZymoBroth Growth Media for efficient transformation workflows to generate highly competent bacterial cells</li>
<li dir="ltr" role="presentation">Overexpression Broth (OB) optimized for increasing recombinant protein yields with high-density nutrient media.</li>
<li dir="ltr" role="presentation">Expansion Broth (EB) for the faster and more reliable expansion of microbial cultures.</li>
<li dir="ltr" role="presentation">Dual Media Sets for integrated growth and induction workflows with the help of combined EB and OB systems</li>
</ul>
&nbsp;
<h2 data-section-id="9xi4x5" data-start="919" data-end="971">How to choose bacterial culture media for cloning</h2>
&nbsp;
LB broth/agar vs. Terrific Broth
LB broth and agar are the standard general-purpose media for routine cloning, colony selection, and small-scale plasmid preparation, while Terrific Broth provides higher nutrient density supporting higher bacterial cell density, making it useful when maximizing plasmid yield in larger-scale preparations.
SOC media for post-transformation recovery
SOC media is richer than standard LB and supplemented with glucose and magnesium. It is commonly used for a brief recovery incubation immediately after heat-shock or electroporation transformation, helping transformed cells recover before plating on selective agar. Some transformation protocols specifically recommend SOC rather than LB for this recovery step.
Antibiotic selection matching
Pre-made antibiotic-selective agar plates must match the resistance marker carried by the transformed plasmid, whether ampicillin, kanamycin, chloramphenicol, or another selection marker. Using the wrong antibiotic&mdash;or no antibiotic at all&mdash;will not properly select for plasmid-containing colonies and may allow unwanted bacterial growth.
Liquid vs. agar plate formats and shelf life
Liquid media (broth) is used for post-transformation recovery and culture scale-up, while agar plates are used for colony isolation and selection. Pre-made antibiotic plates have a defined shelf life because antibiotics gradually lose activity over time, making expiration dates particularly important for antibiotics with lower stability.
&nbsp;
<h2 data-section-id="1fljvas" data-start="3523" data-end="3548">Specifications context</h2>
&nbsp;
LB broth and agar are formulated with defined concentrations of tryptone, yeast extract, and sodium chloride, with agar plates commonly supplied in standard Petri dish sizes (for example, 100 mm) and containing antibiotic concentrations matched to common resistance markers, such as 100 &micro;g/mL ampicillin or 50 &micro;g/mL kanamycin. SOC recovery is typically performed as a short incubation, often around one hour at 37&deg;C with shaking, before plating cells on selective agar.&nbsp;
This sub-category is part of MBP's cloning catalog alongside transformation kits and competent cells, the immediately preceding step, and chemicals/reagents for enzyme-based fragment preparation. Labs scaling up plasmid production for lentivirus packaging in immortalization workflows can also review MBP's cell immortalization catalog. MBP's specialist team can help confirm antibiotic concentration and media format requirements for a specific selection marker before order placement.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today to request a quote for high-quality culture media.

Culture Media

M3011 - MSDS

M3011-Protocol

Dual Media Set™ (100 ml EB™ & 500 ml OB™)

M3011 by Zymo Research is the Dual Media Set™ — Expansion Broth (EB™, 100 mL) plus Overexpression Broth (OB™, 500 mL) — for high-yield E. coli recombinant protein expression using a two-phase workflow that eliminates IPTG induction and OD monitoring in T7-based systems.

Product Storage

<h2>What are methylation standards?</h2>
&nbsp;
Methylation standards are defined reference DNA materials with a known and confirmed methylation status, ranging from a fully methylated DNA (where every cytosine at CpG or other sites carries a methyl group) to a fully unmethylated DNA (where no cytosines are methylated) and intermediate or mixed standards containing a defined ratio of the two. The most widely used unmethylated control in bisulfite sequencing is lambda phage DNA, which is naturally unmethylated and can be spiked into a conversion reaction to estimate conversion efficiency.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Methylated and non-methylated DNA control sets for comparative epigenetic analysis and assay validation</li>
<li dir="ltr" role="presentation">Universal methylated DNA standards for methylation assessment workflows</li>
<li dir="ltr" role="presentation">Genomic DNA reference materials: for microbial and human methylation studies</li>
<li dir="ltr" role="presentation">Matched DNA sets: used in experimental models for controlled comparison of methylation status&nbsp;</li>
<li dir="ltr" role="presentation">Bisulfite-converted DNA standards: for downstream sequencing-based methylation and PCR analysis</li>
<li dir="ltr" role="presentation">Methylation spike-in controls: for sequencing normalization and high-throughput workflows</li>
<li dir="ltr" role="presentation">Mouse and human DNA methylation reference sets: for specific species epigenetic studies</li>
<li dir="ltr" role="presentation">Methylated DNA IP control kits for methylation assays involving immunoprecipitation</li>
</ul>
&nbsp;
<h2>How to use methylation standards</h2>
&nbsp;
Spike an unmethylated control into every bisulfite or enzymatic conversion reaction
Running an unmethylated DNA like lambda phage DNA alongside your samples in the same conversion reaction estimates the conversion efficiency of that specific run, since every remaining cytosine signal after conversion should be treated as a false positive, and this rate can be quantified and accounted for in downstream analysis.
Run a fully methylated positive control to confirm methylated cytosines are preserved
A fully methylated standard, such as DNA synthesized with 5-methyl-dCTP or commercially prepared methylated genomic DNA, confirms your conversion workflow correctly preserves methylated cytosines intact rather than inadvertently converting them, which would produce false-negative methylation calls.
Use a mixed or intermediate standard to calibrate quantitative methylation assays
A standard containing a defined ratio of methylated to unmethylated DNA, or a set of standards spanning a range of methylation percentages, provides a calibration curve for quantitative methylation assays like 5-methylcytosine ELISA, validating that the assay reports the expected methylation level across its full dynamic range.
Confirm standard stability before long-term storage or lot-to-lot use
Methylation control DNA has documented stability windows, and confirming that your standard's methylation status is stable under storage conditions, and consistent across lots, is necessary for reliable longitudinal monitoring of conversion efficiency.
Use standards to monitor workflow consistency over time
Running a conversion control in every batch allows longitudinal tracking of conversion efficiency across instrument runs, reagent lots, and protocol variations, helping catch workflow drift before it affects real sample results.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Lambda phage DNA is the most commonly used unmethylated bisulfite conversion control because it is naturally unmethylated, commercially available, and well characterized, and it can be aligned to the known lambda phage reference genome after sequencing to calculate residual unconverted cytosine rates, providing a direct, interpretable measure of conversion efficiency. As of 2026, fully methylated and fully unmethylated standard sets are also used alongside enzymatic methyl-seq conversions to confirm that EM-seq's enzymatic mechanism correctly protects methylated cytosines and converts unmethylated ones, independent of the conversion chemistry used.
This category sits within DNA/RNA Methylation, alongside Bisulfite Conversion Kits, Non-Bisulfite Kits &amp; Reagents, and ELISA Kits &amp; Reagents. See the full reagents catalog and use Quick Order for recurring orders.
<a href="https://mbpinc.net/contact-us">Contact the expert team of Molecular Biology Products Inc. (MBP)</a> to get high-quality methylation standards for your lab today.

Methylation Standards

E. coli Non-Methylated Genomic DNA (5 µg/20 µl)

D5016 by Zymo Research is 5 µg (250 ng/µL, 20 µL) of fully unmethylated E. coli genomic DNA from Dam–/Dcm– strain ER2925, in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0). Used as a negative control or bisulfite conversion efficiency spike-in in WGBS, RRBS, MSP, and epigenetic array workflows. Store at ≤–20°C.

Genotype

Columns, Plastics & Accessories

Elbow Hose Connector (1 Pack)

Elbow Hose Connector S7004 by Zymo Research is a polypropylene 90-degree connector (3/8&quot; hose barb × 3/8&quot; male pipe thread) for connecting vacuum tubing to the EZ-Vac™ Vacuum Manifold (S7000). Supplied as a single connector.

Color

Dimensions

Material

Plates

Elution Plate (2 Plates)

C2003 — Elution Plate (2 Plates): 96-well V-bottom collection plates with 90 µl per well capacity, for use with Zymo Research spin plate purification systems to collect purified DNA or RNA eluates during high-throughput nucleic acid purification workflows.

<h2>What Are Zymo Research Buffers?</h2>
Zymo Research buffers are formulation-specific reagents used at defined steps &mdash; lysis, binding, wash, and elution &mdash; in Zymo-Spin column-based purification protocols for DNA, RNA, and bisulfite-converted DNA. Each buffer is optimized for a specific kit family: DNA Wash Buffer concentrate (requires ethanol addition before use) removes salts and contaminants before elution from Zymo-Spin silica columns; DNA Elution Buffer (10 mM Tris, pH 8.5, 0.1 mM EDTA) releases purified DNA from the matrix; RNA Wash Buffer supports RNA extraction kits including Quick-RNA and Direct-zol lines; M-Elution Buffer is designed for eluting bisulfite-converted DNA in EZ DNA Methylation kits. Researchers typically order these separately when a single buffer component runs out before the rest of the kit, or when scaling up a protocol beyond the volume included.
TL;DR: Order Zymo buffers individually to replenish a specific step in your existing Zymo workflow without replacing the full kit.
<h2>How to Choose Zymo Research Buffers</h2>
<h3>Match the buffer to your kit family</h3>
Every Zymo buffer is paired to a specific kit line. DNA Wash Buffer and DNA/RNA Wash Buffer concentrate are used in DNA Clean &amp; Concentrator, Quick-DNA, and Zymoclean Gel DNA Recovery kits. RNA Wash Buffer pairs with Quick-RNA kits. ZymoBIOMICS DNA Wash Buffer 1 and Wash Buffer 2 are specific to the ZymoBIOMICS microbiomics line. Substituting a buffer from a different kit family can compromise yield or purity.
<h3>Confirm ethanol addition requirements before use</h3>
Several Zymo wash buffer concentrates require addition of 96-100% ethanol to the bottle before first use &mdash; the label states the exact volume. DNA Wash Buffer concentrate, DNA/RNA Wash Buffer concentrate, and Urine DNA Wash Buffer concentrate all require this step. Pre-made (non-concentrate) versions such as RNA Wash Buffer are supplied ready to use.
<h3>Select the right volume for your throughput</h3>
Zymo buffers are available in sizes from 6 ml (single-experiment replenishment) up to 500 ml (Buffer P1 Red, for high-volume plasmid prep labs). For labs processing more than 50 samples per week, ordering the largest available size reduces per-sample cost and the frequency of reordering.
<h3>Choose elution buffer chemistry based on downstream application</h3>
DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA) is the standard for most PCR and sequencing applications. For applications sensitive to EDTA &mdash; such as some restriction enzyme digests or direct use with divalent cation-dependent enzymes &mdash; eluting in molecular-grade water or a Tris-only buffer is preferable. M-Elution Buffer is specifically formulated for bisulfite-converted DNA and should not be substituted with standard elution buffer in methylation workflows.
<h3>Verify research-use-only status</h3>
All Zymo Research buffers sold through MBP are for research use only (RUO). They are not cleared for in vitro diagnostic use. For clinical or diagnostic laboratory applications, confirm regulatory status with your institutional compliance officer before use.
<h2>Compare Zymo Buffer Types</h2>
<table>
<thead>
<tr>
<th>Buffer Type</th>
<th>Primary Application</th>
<th>Kit Family</th>
<th>Format Available</th>
</tr>
</thead>
<tbody>
<tr>
<td>DNA Wash Buffer (concentrate)</td>
<td>Salt removal before DNA elution</td>
<td>DNA Clean &amp; Concentrator, Quick-DNA</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA/RNA Wash Buffer (concentrate)</td>
<td>Salt removal for dual DNA/RNA preps</td>
<td>Quick-DNA/RNA kits</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA)</td>
<td>DNA release from silica column</td>
<td>All Zymo DNA purification</td>
<td>16 ml &ndash; available sizes vary</td>
</tr>
<tr>
<td>RNA Wash Buffer</td>
<td>RNA purification wash step</td>
<td>Quick-RNA kits</td>
<td>Ready-to-use</td>
</tr>
<tr>
<td>M-Elution Buffer</td>
<td>Elution of bisulfite-converted DNA</td>
<td>EZ DNA Methylation kit families</td>
<td>Small volume</td>
</tr>
<tr>
<td>ZymoBIOMICS DNA Wash Buffer 2</td>
<td>Metagenomic/microbiomic DNA cleanup</td>
<td>ZymoBIOMICS DNA kits</td>
<td>Sold separately</td>
</tr>
<tr>
<td>Buffer P1 Red</td>
<td>Alkaline lysis resuspension (plasmid prep)</td>
<td>Zyppy Plasmid Miniprep</td>
<td>10 ml &ndash; 500 ml</td>
</tr>
</tbody>
</table>
<h2>Specifications Context</h2>
Zymo wash buffer concentrates are supplied at a defined fold-concentration (commonly 4x or 6x) and require ethanol addition to reach working strength &mdash; the exact ratio is printed on the bottle and in the kit protocol. Elution buffer volumes for Zymo-Spin columns typically range from 6 to 100 &micro;l per elution; larger elution volumes improve total yield at the cost of DNA concentration. For DNA elution from Zymo-Spin columns, the protocol recommends a minimum 1-minute contact time between elution buffer and the silica matrix to maximize recovery of fragments 10 kb and above. As of 2025, Zymo Research tests all buffer lots for performance consistency, with integrity guaranteed for up to one year from purchase date.
Browse the full <a href="https://www.notion.so/reagents">Zymo Research reagents collection at MBP</a> for kits and columns that use these buffers, or visit our <a href="https://www.notion.so/reagents/buffers">reagent buffers category</a> for non-Zymo buffer options including ABM and other brands. For volume orders or purchase orders, use the <a href="https://www.notion.so/quickorder">Quick Order</a> tool or <a href="https://www.notion.so/contact-us">contact us</a> directly &mdash; MBP is a registered vendor for institutional procurement systems.
Last updated: June 2025
&nbsp;

Zymo Buffers

Endo-Wash Buffer

Zymo Research Endo-Wash Buffer: endotoxin (LPS) removal wash for Zyppy Plasmid Miniprep and ZR Plasmid Miniprep kits. Seven volumes: D4036-3-6 (6 mL), D4036-3-15 (15 mL), D4036-3-30 (30 mL), D4036-3-60 (60 mL), D4036-3-120 (120 mL), D4036-3-160 (160 mL), D4036-3-240 (240 mL). Room temp storage.

Endo-Wash Buffer (120 ml)

Endo-Wash Buffer (15 ml)

Endo-Wash Buffer (160 ml)

Endo-Wash Buffer (240 ml)

Endo-Wash Buffer (30 ml)

Endo-Wash Buffer (6 ml)

Endo-Wash Buffer (60 ml)

Spin Columns

EndoZero II Spin Columns (25 Pack)

C1060-25 by Zymo Research is a 25-pack of EndoZero™ II Spin Columns for polishing plasmid DNA purified with ZymoPURE II Midiprep, Maxiprep, or Gigaprep kits to ≤0.025 EU/µg endotoxin — 40× lower than standard transfection-grade. Accepts up to 400 µg plasmid input; 2-minute incubation + centrifugation.

EndoZero Spin Columns

Zymo Research EndoZero Spin Columns for LPS removal from plasmid DNA — for use with ZymoPURE II Maxi and Gigaprep kits. Two pack sizes: C1051-5 (5 columns) and C1051-10 (10 columns).

EndoZero Spin Columns (10 Pack)

EndoZero Spin Columns (5 Pack)

M3012-100 - MSDS

Expansion Broth™ (EB™)

Expansion Broth (EB) by Zymo Research: bacterial growth medium that represses recombinant protein expression during the cell expansion phase. Use with Overexpression Broth (OB) for consistent, high-yield recombinant protein production. Room temperature storage. 100 ml and 500 ml sizes available. Available in 100mL (M3012-100) and 500mL (M3012-500).

Expansion Broth™ (EB™) (100 ml)

Expansion Broth™ (EB™) (500 ml)

<h2>What are bisulfite conversion kits?</h2>
&nbsp;
Bisulfite conversion kits are all-in-one systems that bundle every reagent and column needed for the sodium bisulfite conversion workflow into a single, pre-optimized format, covering the denaturation and sulfonation steps, desulfonation with an alkali reagent, and a post-conversion cleanup step that removes the bisulfite and other reaction byproducts before the converted DNA is used in downstream analysis. This contrasts with standalone Bisulfite Conversion Reagents &amp; Buffers, which lets labs assemble their own optimized protocol from individual components.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Standard bisulfite conversion kits for routine DNA methylation analysis&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Combined transcriptomic and epigenetic studies with RNA and DNA methylation conversion kits</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">High-throughput 96-well bisulfite conversion kits for wider-scale screening applications</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Automation-compatible bisulfite kits for simplified workflow integration</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Magnetic bead-based bisulfite conversion kits for enhanced purification and recovery efficiency</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Direct bisulfite conversion kits for streamlined sample processing&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Rapid conversion kits for methylation analysis workflows that are time-sensitive&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Starter kits for validation and optimized setup of bisulfite conversion protocols</li>
</ul>
&nbsp;
<h2>How to choose a bisulfite conversion kit</h2>
&nbsp;
Match kit format to your downstream sequencing or PCR application
Kit versions optimized for WGBS work at larger input scales with library prep in mind, while RRBS-compatible kits often include restriction enzyme digestion components to enrich CpG-dense regions before conversion, and MSP-focused kits may include primers or protocol guidance for post-conversion methylation-specific PCR.
Check the kit's validated input DNA range against your sample type
Different bisulfite conversion kits are validated for different input DNA ranges, and kits validated for low-input or cell-free DNA samples specifically address the additional fragmentation and yield challenges that these sample types face during the harsh chemical conversion step.
Confirm the kit includes an unmethylated conversion control or specify one separately
A quality bisulfite conversion kit should provide, or clearly specify, an unmethylated control to run alongside samples, since tracking conversion efficiency across the kit's components helps catch kit-specific variability in conversion completeness between different lots or runs.
Consider kits with optimized FFPE protocols when working with formalin-fixed tissue
Formalin-fixed, paraffin-embedded tissue DNA is already fragmented before conversion; kits with FFPE-specific protocol variants include modifications like shortened incubation times and carrier reagents to minimize the further fragmentation this already-compromised input type would suffer under standard conversion conditions.
Evaluate throughput format options to match your sample volume
Some bisulfite conversion kits are available in spin column formats suited to individual sample processing, while plate-based or automation-compatible formats support higher-throughput sample sets, making format an important practical consideration beyond kit chemistry alone.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Bisulfite sequencing is widely regarded as the gold standard for DNA methylation analysis at single-base resolution, and a key advantage of using a complete kit over standalone reagents is that the included cleanup step reliably removes residual bisulfite and salt from the converted DNA, which would otherwise inhibit downstream PCR amplification and library preparation. As of 2026, enzymatic conversion kits (covered in Non-Bisulfite Kits &amp; Reagents) are increasingly positioned as an alternative that avoids the DNA damage introduced by chemical bisulfite treatment, though bisulfite conversion kits remain broadly used and well-validated across the largest range of downstream applications.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP and get high-quality bisulfite conversion kits for your lab.

Bisulfite Conversion Kits

Data Sheet

EZ DNA Methylation-Direct™ Kit

Zymo Research EZ DNA Methylation-Direct™ Kit: &gt;99.5% bisulfite conversion directly from blood, tissue, cells, FFPE, or LCM — no prior DNA purification required. 50 pg–2 µg or 10–10⁵ cells; ≥10 µl. D5020 (50 rxns) and D5021 (200 rxns).