Zymo Research

<h2>What are Magnetic Bead Total RNA Extraction Kits?</h2>
&nbsp;
Magnetic bead total RNA extraction kits isolate total RNA by adsorbing nucleic acids onto paramagnetic silica-coated beads in a high-chaotropic-salt lysis buffer, then using a magnetic field to capture and wash beads without centrifugation. Unlike standard spin-column kits, the bead format removes centrifuge dependency, enabling scalable manual processing in 96-well plates or full automation on liquid-handling robots.&nbsp;Choose a magnetic bead format when your lab processes more than 48 samples per day or when a Tecan, Hamilton, or Beckman Biomek platform is available.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Magbio HighPrep Kits for liquid biopsy collection systems and non-invasive swabbing using rapid magnetic bead isolation&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Zymo-Seq RiboFree&trade; Kits to eliminate ribosomal RNA carryover during library synthesis
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Direct-zol-96 MagBead RNA Modules facilitate complete avoidance of manual phase-separation steps while enabling automated transcript extraction directly from TRIzol&reg; lysates&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Magbio HighPrep Total RNA Plus Kit extracts pure, inhibitor-free RNA from complex samples with maximum binding capacity&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Quick-RNA MagBead Packs deliver high-integrity total transcripts without dependence on centrifuge spins or vacuum manifold.
</li>
</ul>
&nbsp;
<h2>How to Choose a Magnetic Bead Total RNA Extraction Kit</h2>
&nbsp;
Automation Compatibility
Confirm that the kit supplier provides automation scripts for your specific platform. The Zymo Research Quick-DNA/RNA Viral MagBead kit and Thermo Fisher MagMAX-96 both offer downloadable instrument scripts for Tecan and Hamilton systems. Generic magnetic bead kits without published automation protocols require in-house script development, adding significant setup time.
Small RNA Retention
Bead kits that bind RNA &gt;=17 nt co-purify microRNAs alongside mRNA and rRNA from the same input. Kits specifying &gt;=200 nt retention exclude small RNAs and are not appropriate for miRNA expression panels. Check the size cutoff stated in the kit datasheet before selecting for non-coding RNA work.
Input Sample Type
Magnetic bead kits for total RNA support cell pellets, tissue homogenates, blood, bacteria, yeast, and fungi. For cell-free matrices (plasma, serum, nasal wash), switch to a viral-RNA or liquid-biopsy magnetic bead kit with included carrier RNA to maximize recovery from low-input, cell-free samples.
Elution Volume and Concentration
Standard magnetic bead kits elute into 50-100 ul; some protocols allow concentration into 20-50 ul, increasing RNA concentration for sensitive downstream applications. If downstream libraries require &gt;=200 ng/ul input, reduce elution volume accordingly and verify the kit supports this protocol variant.
gDNA Removal
Confirm whether the kit includes on-bead DNase I or requires a separate gDNA digestion step. For RT-qPCR and RNA-seq, omitting DNase treatment introduces a genomic DNA signal that inflates expression measurements.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
RNA purity targets from magnetic bead extractions match spin-column benchmarks: A260/A280 &gt;= 1.8 and A260/A230 &gt;= 1.5. RNA integrity number (RIN) &gt;= 7 is recommended for RNA-seq library preparation; magnetic bead kits produce RIN values in the 8-10 range for fresh samples when lysis is performed immediately on ice. Bead-based workflows are cited in published automation protocols from genomics core facilities and are increasingly specified by NIH-funded projects requiring standardized sample processing across multi-site studies.
<section class="mbp-contact-cta"></section>
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP Inc. to get automation-compatible mag bead selections today.

Mag Beads

MSDS

R2100-MSDS

DataSheet

Protocol

Direct-zol-96 MagBead RNA

Direct-zol-96 MagBead RNA by Zymo Research — magnetic bead 96-well RNA purification from TRI Reagent homogenates. Up to 10 µg binding, ≥17 nt, A260/A280 &gt;1.8, ≥30 µl elution. Available as R2100 (1×96 Preps), R2101 (2×96 + TRI Reagent), R2102 (4×96 Preps), and R2103 (4×96 + TRI Reagent).

Compatibility

Equipment

Sample Inactivation

Sample Source

Size Range

Yield

Manufacturer

Shipping Condition

Storage Condition

Direct-zol™-96 MagBead RNA (1 x 96 Preps.)

Direct-zol™-96 MagBead RNA w/TRI Reagent® (2 x 96 Preps.) R2101-A and R2050-1-200

Direct-zol™-96 MagBead RNA (4 x 96 Preps.)

Direct-zol™-96 MagBead RNA w/TRI Reagent® (4 x 96 Preps.) R2103-A and 2 x R2050-1-200

<h2>What are 96-Well Plate Total RNA Extraction Kits?</h2>
&nbsp;
96-well plate total RNA extraction kits purify total RNA from up to 96 samples in a single processing run using silica-membrane plates, glass-fiber filter plates, or magnetic bead formats compatible with centrifuges, vacuum manifolds, or automated liquid handlers. Unlike single-tube spin-column formats, plate kits maintain individual sample integrity while delivering the throughput needed for screens, longitudinal studies, and genomics pipelines processing hundreds of samples per week.&nbsp;Choose a 96-well plate format when processing 48 or more samples per session to eliminate the per-sample handling overhead of individual spin columns.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Direct-zol&trade;-96 RNA Kits facilitate bypass of the phase-separation steps, enabling phenol-free, high-throughput transcript extraction directly from TRIzol&reg; reagents&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Quick-RNA&trade; 96 Kits for rapid, ultra-pure total RNA delivery from small-scale cellular samples
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
96-well Tissue RNA Kits with multi-well lysis arrays to handle complex, fibrous animal tissues without blocking the filtration matrices
</li>
</ul>
&nbsp;
<h2>How to Choose a 96-Well Plate RNA Extraction Kit</h2>
&nbsp;
Processing Method: Centrifuge vs. Vacuum vs. Magnet
Centrifuge-compatible plates (e.g., QIAGEN RNeasy 96) require a 96-well plate rotor; vacuum manifold protocols skip that equipment dependency. Magnetic bead formats eliminate both and are the preferred choice when an automation platform is available. Select based on the equipment already in your facility before comparing kits.
Sample Type and Starting Amount
Standard plate kits handle 100-2x10^6 cultured mammalian cells or up to 30 mg of tissue per well. Plant tissue extraction requires an additional plant isolation aid to remove polysaccharide and polyphenol inhibitors. Do not use total RNA kits for cell-free samples (plasma, serum, nasal wash) - those require viral RNA formats.
On-Plate DNase Treatment
For RT-qPCR and RNA-seq, choose kits that include on-plate DNase I digestion to remove genomic DNA contamination. Most silica-membrane kits supply lyophilized DNase I; verify that the kit includes this reagent rather than requiring a separate purchase.
Elution Volume and Concentration
96-well kits typically elute in 50-100 ul per well; magnetic bead formats can concentrate into 20-50 ul, which is advantageous for low-abundance samples or applications needing high RNA concentration for library prep.
Automation Compatibility
Magnetic bead 96-well formats integrate with Tecan, Hamilton, and Beckman Coulter liquid handlers using vendor-supplied scripts. Silica-membrane plates can be semi-automated on vacuum manifold platforms. Confirm the kit supplier provides an automation protocol before purchase if robotic processing is required.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Key purity benchmarks for 96-well RNA eluates are A260/A280 &gt;= 1.8 and A260/A230 &gt;= 1.5; values below these thresholds indicate carryover of protein, phenol, or guanidinium salts that inhibit downstream enzymes. Silica-membrane kits from QIAGEN and Bio-Rad remain the most widely cited 96-well formats in peer-reviewed methods, and their protocols are referenced directly in published automation workflows at institutions including the Howard Hughes Medical Institute. RNase-free reagents and consumables are required throughout to prevent RNA degradation; confirm kit buffers are certified RNase-free before use with precious or low-abundance samples.
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

96-well plate

Direct-zol-96 RNA Kits

The Direct-zol&#8482;-96 RNA Kit by Zymo Research is available in four configurations — R2054 (2 × 96 preps), R2055 (2 × 96 preps with TRI Reagent), R2056 (4 × 96 preps), and R2057 (4 × 96 preps with TRI Reagent) — for high-throughput total RNA purification directly from TRI Reagent or compatible acid-guanidinium-phenol lysates without chloroform extraction or precipitation. With a 10 µg binding capacity per well, phenol-free RNA at A260/A280 &gt;1.8, and recovery of all RNAs ≥17 nt including miRNA, these kits are suited for NGS, RT-qPCR, and small RNA profiling across cell, tissue, and biological fluid inputs.

Direct-zol™-96 RNA (2 x 96 Preps.)

Direct-zol™-96 RNA w/TRI Reagent® (2 x 96 Preps.) R2055-A and R2050-1-200

Direct-zol™-96 RNA (4 x 96 Preps.)

Direct-zol™-96 RNA w/TRI Reagent® (4 x 96 Preps.) R2057-A and 2 x R2050-1-200

<h2>What Are Spin-Column Total RNA Kits?</h2>
&nbsp;
Spin-column total RNA kits are the most widely used format for manual RNA purification in molecular biology laboratories. The technology relies on silica membrane chemistry: under chaotropic (guanidinium-based) lysis conditions, RNA adsorbs selectively to the silica membrane of the column; sequential ethanol-based wash steps remove proteins, salts, and gDNA fragments; and a low-ionic-strength buffer or RNase-free water then releases the purified RNA. Standard mini-format kits accept cell or tissue lysate from inputs of 1x10^4-1x10^7 cells or 5-100 mg tissue, bind up to 100 ug RNA, and complete the protocol in 15-30 minutes. The eluted RNA is suitable for RT-PCR, qRT-PCR, RNA sequencing, Northern blot, and hybridization without additional cleanup.&nbsp;Spin-column RNA kits are the default choice for 1-24 samples per run in any lab that does not require automated magnetic-bead processing.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Quick-RNA Microprep and Miniprep Kits for low-volume elution and highly concentrated recovery, providing ultra-clean and phenol-free transcript isolation
</li>
<li dir="ltr" aria-level="1">
Direct-zol&trade; RNA Kits to bypass tedious phase separation steps, allowing researchers to pump TRIzol-lysed samples directly into spin-columns&nbsp;
</li>
<li dir="ltr" aria-level="1">
Direct-zol&trade; DNA/RNA Parallel Modules are engineered for the non-destructive, simultaneous isolation of DNA and RNA fractions.
</li>
<li dir="ltr" aria-level="1">
ZymoBIOMICS&trade; RNA Mini Kits for uniform microbial profiling featuring host-zeroing lysis mechanics
</li>
<li dir="ltr" aria-level="1">
Quick-RNA&trade; Midiprep Assemblies to capture larger quantities of high-purity total transcripts efficiently, tailored for bulk processing&nbsp;
</li>
</ul>
&nbsp;
<h2>How to Choose Spin-Column Total RNA Kits</h2>
&nbsp;
Column Binding Capacity and Elution Volume
Mini-format columns bind up to 100 ug total RNA and elute in 10-100 uL. For samples with very low RNA content (needle biopsies, laser-capture microdissection sections, small cell numbers &lt;=10^4), choose micro-format columns with 8-12 uL minimum elution volume to maximize concentration. Eluting in &lt;30 uL with high RNA concentration increases sensitivity in downstream RT-qPCR.
Small RNA Retention
This is the most important differentiator between kit brands. Kits using Zymo-Spin column technology with proprietary binding buffers retain miRNA and small RNA species &gt;=17 nt in the total RNA fraction. Competing kits using standard high-ethanol wash chemistry typically lose RNA &lt;200 nt in the wash steps. If your workflow includes small RNA profiling, miRNA RT-qPCR, or small RNA-seq, verify that the kit retains &gt;=17 nt RNA before purchasing.
On-Column DNase Treatment
Included RNase-free DNase I that degrades gDNA on the column during the wash stage eliminates the need for post-elution DNase treatment. This matters for RT-qPCR with primers spanning a small intron, or for RNA-seq where even trace gDNA contamination generates false mapping reads. DNase-treated RNA typically passes a no-RT PCR control at Ct &gt;35.
Sample Type: Cells vs Tissue
Cell lysis buffer volumes and homogenization requirements differ by sample type. Cultured cells lyse directly in lysis buffer with vortexing; tissue samples require homogenization to disrupt cell structure before contact with the lysis buffer. For fibrous tissues (muscle, heart), use a bead-mill homogenizer or rotor-stator; for soft tissues (liver, brain), a disposable pestle or needle shearing is sufficient. Some kits include TissueLyser-compatible bead tubes or QIAshredder-equivalent homogenizer columns.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Spin-column total RNA purity targets: A260/A280 ratio 1.8-2.1; A260/A230 ratio &gt;1.8. RNA integrity number (RIN) from fresh cultured cells or snap-frozen tissue is routinely 8-10 with validated kits. Zymo Research Quick-RNA Mini and Miniprep kits are among the most cited spin-column RNA isolation kits in published RT-PCR and RNA-seq methods; ABM RNA extraction kits offer validated protocols for bacteria, yeast, and fungi not covered by many standard cell/tissue kits. All kits supplied by MBP are Research Use Only (RUO).
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Spin-column

Direct-zol™ DNA/RNA Miniprep Kit

Direct-zol™ DNA/RNA Miniprep Kit by Zymo Research co-purifies genomic DNA (>40 kb, 25 µg capacity) and total RNA (≥17 nt, 50 µg capacity) simultaneously from TRIzol or TRI Reagent-lysed samples in a single column workflow. Ideal for limited samples and integrated multi-omics studies. Available in 10 preps (R2080T) or 50 preps (R2080), with a TRI Reagent bundle also available at 50 preps (R2081).

Direct-zol™ DNA/RNA Miniprep Kit (50 Preps.)

Direct-zol™ DNA/RNA Miniprep (10 Preps.)

Direct-zol™ DNA/RNA Miniprep Kit w/TRI Reagent® (50 Preps.) R2081-A and R2050-1-50

Direct-zol™ RNA MiniPrep

Direct-zol™ RNA MiniPrep by Zymo Research. 50 µg binding capacity. Elution ≥25 µl. RNA ≥17 nt. 7-minute protocol. Purify total RNA directly from TRI Reagent without phase separation. Available formats: R2051 (50 preps + TRI Reagent), R2051-A (50 preps, alternate TRI bundle), R2050-1-50 (TRI Reagent 50 ml), R2052 (200 preps), R2053 (200 preps + TRI Reagent), R2053-A (200 preps, alternate TRI bundle), R2050-1-200 (TRI Reagent 200 ml).

Direct-zol™ RNA MiniPrep w/TRI Reagent® (50 Preps.) R2051-A and R2050-1-50

Direct-zol™ RNA MiniPrep (200 Preps.)

Direct-zol™ RNA MiniPrep w/TRI Reagent® (200 Preps.) R2053-A and R2050-1-200

<h2>What Are Zymo Research Buffers?</h2>
Zymo Research buffers are formulation-specific reagents used at defined steps &mdash; lysis, binding, wash, and elution &mdash; in Zymo-Spin column-based purification protocols for DNA, RNA, and bisulfite-converted DNA. Each buffer is optimized for a specific kit family: DNA Wash Buffer concentrate (requires ethanol addition before use) removes salts and contaminants before elution from Zymo-Spin silica columns; DNA Elution Buffer (10 mM Tris, pH 8.5, 0.1 mM EDTA) releases purified DNA from the matrix; RNA Wash Buffer supports RNA extraction kits including Quick-RNA and Direct-zol lines; M-Elution Buffer is designed for eluting bisulfite-converted DNA in EZ DNA Methylation kits. Researchers typically order these separately when a single buffer component runs out before the rest of the kit, or when scaling up a protocol beyond the volume included.
TL;DR: Order Zymo buffers individually to replenish a specific step in your existing Zymo workflow without replacing the full kit.
<h2>How to Choose Zymo Research Buffers</h2>
<h3>Match the buffer to your kit family</h3>
Every Zymo buffer is paired to a specific kit line. DNA Wash Buffer and DNA/RNA Wash Buffer concentrate are used in DNA Clean &amp; Concentrator, Quick-DNA, and Zymoclean Gel DNA Recovery kits. RNA Wash Buffer pairs with Quick-RNA kits. ZymoBIOMICS DNA Wash Buffer 1 and Wash Buffer 2 are specific to the ZymoBIOMICS microbiomics line. Substituting a buffer from a different kit family can compromise yield or purity.
<h3>Confirm ethanol addition requirements before use</h3>
Several Zymo wash buffer concentrates require addition of 96-100% ethanol to the bottle before first use &mdash; the label states the exact volume. DNA Wash Buffer concentrate, DNA/RNA Wash Buffer concentrate, and Urine DNA Wash Buffer concentrate all require this step. Pre-made (non-concentrate) versions such as RNA Wash Buffer are supplied ready to use.
<h3>Select the right volume for your throughput</h3>
Zymo buffers are available in sizes from 6 ml (single-experiment replenishment) up to 500 ml (Buffer P1 Red, for high-volume plasmid prep labs). For labs processing more than 50 samples per week, ordering the largest available size reduces per-sample cost and the frequency of reordering.
<h3>Choose elution buffer chemistry based on downstream application</h3>
DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA) is the standard for most PCR and sequencing applications. For applications sensitive to EDTA &mdash; such as some restriction enzyme digests or direct use with divalent cation-dependent enzymes &mdash; eluting in molecular-grade water or a Tris-only buffer is preferable. M-Elution Buffer is specifically formulated for bisulfite-converted DNA and should not be substituted with standard elution buffer in methylation workflows.
<h3>Verify research-use-only status</h3>
All Zymo Research buffers sold through MBP are for research use only (RUO). They are not cleared for in vitro diagnostic use. For clinical or diagnostic laboratory applications, confirm regulatory status with your institutional compliance officer before use.
<h2>Compare Zymo Buffer Types</h2>
<table>
<thead>
<tr>
<th>Buffer Type</th>
<th>Primary Application</th>
<th>Kit Family</th>
<th>Format Available</th>
</tr>
</thead>
<tbody>
<tr>
<td>DNA Wash Buffer (concentrate)</td>
<td>Salt removal before DNA elution</td>
<td>DNA Clean &amp; Concentrator, Quick-DNA</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA/RNA Wash Buffer (concentrate)</td>
<td>Salt removal for dual DNA/RNA preps</td>
<td>Quick-DNA/RNA kits</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA)</td>
<td>DNA release from silica column</td>
<td>All Zymo DNA purification</td>
<td>16 ml &ndash; available sizes vary</td>
</tr>
<tr>
<td>RNA Wash Buffer</td>
<td>RNA purification wash step</td>
<td>Quick-RNA kits</td>
<td>Ready-to-use</td>
</tr>
<tr>
<td>M-Elution Buffer</td>
<td>Elution of bisulfite-converted DNA</td>
<td>EZ DNA Methylation kit families</td>
<td>Small volume</td>
</tr>
<tr>
<td>ZymoBIOMICS DNA Wash Buffer 2</td>
<td>Metagenomic/microbiomic DNA cleanup</td>
<td>ZymoBIOMICS DNA kits</td>
<td>Sold separately</td>
</tr>
<tr>
<td>Buffer P1 Red</td>
<td>Alkaline lysis resuspension (plasmid prep)</td>
<td>Zyppy Plasmid Miniprep</td>
<td>10 ml &ndash; 500 ml</td>
</tr>
</tbody>
</table>
<h2>Specifications Context</h2>
Zymo wash buffer concentrates are supplied at a defined fold-concentration (commonly 4x or 6x) and require ethanol addition to reach working strength &mdash; the exact ratio is printed on the bottle and in the kit protocol. Elution buffer volumes for Zymo-Spin columns typically range from 6 to 100 &micro;l per elution; larger elution volumes improve total yield at the cost of DNA concentration. For DNA elution from Zymo-Spin columns, the protocol recommends a minimum 1-minute contact time between elution buffer and the silica matrix to maximize recovery of fragments 10 kb and above. As of 2025, Zymo Research tests all buffer lots for performance consistency, with integrity guaranteed for up to one year from purchase date.
Browse the full <a href="https://www.notion.so/reagents">Zymo Research reagents collection at MBP</a> for kits and columns that use these buffers, or visit our <a href="https://www.notion.so/reagents/buffers">reagent buffers category</a> for non-Zymo buffer options including ABM and other brands. For volume orders or purchase orders, use the <a href="https://www.notion.so/quickorder">Quick Order</a> tool or <a href="https://www.notion.so/contact-us">contact us</a> directly &mdash; MBP is a registered vendor for institutional procurement systems.
Last updated: June 2025
&nbsp;

Zymo Buffers

Direct-zol™ RNA PreWash (Concentrate)

Direct-zol™ RNA PreWash Concentrate R2050-2-160 by Zymo Research — 160 ml, room-temperature stable wash buffer for all Direct-zol RNA column-based kits. Removes TRI Reagent and phenol carryover from silica columns during RNA purification. Concentrate: requires preparation per kit protocol before first use. Also available in 40 ml (R2050-2-40). Order via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.

Direct-zol™ RNA PreWash (Concentrate) (160 ml)

Direct-zol™ RNA PreWash (Concentrate) (40 ml)

<h2>What are DNA Cleanup Kits?</h2>
&nbsp;
DNA cleanup kits purify and concentrate pre-extracted or pre-amplified DNA by removing enzymes, salts, organic solvents (phenol, chloroform), unincorporated nucleotides, RNA, detergents, and other contaminants from DNA solutions using silica-membrane spin columns with a bind-wash-elute protocol. Unlike PCR cleanup kits that are specifically optimized to remove primers and dNTPs from PCR reactions, DNA cleanup kits accept broader input chemistries - including post-phenol-chloroform extraction DNA, genomic DNA in high-salt lysis buffers, restriction digest reactions, and dilute DNA solutions requiring concentration - and elute DNA in minimal volumes (as little as 6 ul) for immediate use in downstream applications. Use a DNA cleanup kit whenever your DNA preparation contains inhibitors incompatible with downstream enzymatic reactions, when residual phenol must be removed, or when dilute DNA must be concentrated into a small volume.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
DNA Clean &amp; Concentrator&trade; (DCC): Efficient spin-column systems (5, 25, 100, 500 preps) created to concentrate low-concentration DNA into ultra-pure, transfection-quality volumes.
</li>
<li dir="ltr" aria-level="1">
Genomic DNA Clean &amp; Concentrator: Tailored procedures for extracting large templates while maintaining genomic integrity for long-read sequencing uses.
</li>
<li dir="ltr" aria-level="1">
Select-a-Size DNA Clean &amp; Concentrator&trade;: Tailored systems for the recovery of size-specific fragments, efficiently eliminating adapter dimers and non-target amplicons.
</li>
<li dir="ltr" aria-level="1">
ZR-96 DNA Cleanup Systems: Scalable 96-well and MagBead configurations designed for high-throughput, automated processes while ensuring reproducibility-validated outcomes.
</li>
</ul>
&nbsp;
<h2>How to Choose a DNA Cleanup Kit</h2>
&nbsp;
Capacity and Input Volume
Zymo DNA Clean &amp; Concentrator kits are available in formats matched to DNA input mass: DCC-5 (&lt;=5 ug input, elute &gt;=6 ul), DCC-25 (&lt;=25 ug input, elute &gt;=25 ul), DCC-100 (&lt;=100 ug input, elute &gt;=25 ul). Choose the format whose maximum capacity exceeds your expected DNA input by at least 2-fold to avoid column overloading, which reduces yield and purity. For genomic DNA preparations from tissue (typically 5-30 ug), DCC-25 or DCC-100 is appropriate.
Minimum Elution Volume and Concentration
For maximum DNA concentration, Zymo DCC-5 elutes in 6 ul - the industry minimum for standard silica columns. If the input volume is 500 ul (1 ng/ul) and the elution is 6 ul, the final concentration reaches approximately 80 ng/ul (assuming 95% recovery), a concentration suitable for transfection, electroporation, and microinjection.
Size Selectivity
Standard DNA cleanup kits retain all DNA &gt;=100 bp. If size selection is also required, use gel extraction (for specific band isolation) or AMPure XP beads (for size-range selection by bead: volume ratio adjustment) rather than a standard cleanup kit.
Post-Phenol-Chloroform Cleanup
For DNA recovered from phenol-chloroform extraction, add 5 volumes of binding buffer and 2 volumes of 100% ethanol to the aqueous phase before column loading. The organic solvent in residual phenol or chloroform does not significantly impair column binding at these dilutions. Multiple column reloads allow processing of large aqueous-phase volumes through a single column.
RNase A Treatment for DNA-Only Preparations
For gDNA preparations that must be RNA-free for optical mapping or certain long-read sequencing library kits, add RNase A (100 ug/ml) to the DNA solution, incubate at 37 degrees C for 10 minutes, then proceed directly to DCC cleanup. The RNase digestion products are removed during the wash steps.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Zymo DNA Clean &amp; Concentrator kits use a single-buffer system with Zymo-Spin column technology and recover DNA from PCR, enzymatic reactions, phenol-chloroform extractions, and dilute solutions. DNA is eluted in volumes as small as 6 ul, providing up to 80-fold concentration from 500 ul inputs. The kits remove enzymes, proteins, salts, organic solvents, dyes, unincorporated nucleotides, primers, and other small molecules, delivering DNA ready for restriction digestion, ligation, sequencing, Southern blotting, transfection, and long-read library preparation. DNA cleanup and concentration are standard workflow steps in genomics laboratories at Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center, where removing trace inhibitors from gDNA before library preparation significantly improves NGS library complexity and uniformity.
&nbsp;
Enhance your capabilities&mdash;<a href="https://mbpinc.net/contact-us">reach out to the MBP Inc. team</a> now to obtain a quote for your DNA concentration requirements.

DNA Cleanup Kits

Brochure

DNA Clean & Concentrator Magbead Kit (100 preps)

D4012 by Zymo Research is a 100-prep magnetic bead DNA cleanup kit compatible with any open liquid handler or magnetic bead mover. Recovers DNA ≥25 bp (up to 150 kb HMW); &gt;90% recovery for ≥100 bp fragments; purity A260/A280 &amp; A260/A230 ≥1.8; elution in 30–50 µl. Suitable for PCR cleanup, NGS library prep, restriction digests, and automated high-throughput purification.

Binding Capacity

Purity

Sample Sources

Size

DNA Clean & Concentrator-100

The DNA Clean &amp; Concentrator-100 by Zymo Research purifies up to 100 µg of DNA from PCR, large restriction digests, or cell-free lysates in &lt;20 min, with 70–95% recovery for 50 bp–10 kb and ≥150 µL elution. Available as D4029 (25 Preps.) and D4030 (50 Preps.).

Detergent Tolerance

Elution Volume

DNA Clean & Concentrator™-100 (25 Preps.)

DNA Clean & Concentrator™-100 (50 Preps.)

DNA Clean & Concentrator-25

Zymo Research DNA Clean &amp; Concentrator-25: 25 µg binding capacity, ≥25 µl elution, ~2 min protocol, 70–90% recovery (50 bp–10 kb). Two sizes: D4033 (50 preps) and D4034 (200 preps).

DNA Clean & Concentrator™-25 (50 Preps.)

DNA Clean & Concentrator™-25 (200 Preps.)

Zymo Research DNA Clean &amp; Concentrator-25: 25 µg binding, ≥25 µl elution, ~2 min protocol, 70–90% recovery (50 bp–10 kb). D4005 (50 preps) and D4006 (200 preps).

DNA Clean & Concentrator-500

The DNA Clean &amp; Concentrator™-500 by Zymo Research purifies and concentrates up to 500 µg of DNA from large-scale restriction digests, crude preparations, and high-mass PCR reactions in under 20 minutes, with 70–95% recovery for 50 bp–10 kb fragments. Available as D4031 (10 Preps.) and D4032 (20 Preps.).

DNA Clean & Concentrator™-500 (10 Preps.)

DNA Clean & Concentrator™-500 (20 Preps.)

DNA Clean & Concentrator™-5

DNA Clean & Concentrator-5 by Zymo Research — 2-minute cleanup; ≥6 µL elution; 50 bp–23 kb; ≤5 µg binding capacity; A260/A280 > 1.8; removes enzymes, dNTPs, and buffers from PCR and enzymatic reactions. Available in 10/50/200-prep kits (uncapped or capped columns), or individual Binding and Wash buffers.

DNA Clean & Concentrator™-5 (50 Preps.) (Uncapped)

DNA Clean & Concentrator™-5 Kit (10 Preps.)(Uncapped)

DNA Clean & Concentrator™-5 (200 Preps.)(Uncapped)

DNA Clean & Concentrator™-5 (50 Preps.) (Capped)

DNA Clean & Concentrator™-5 (200 Preps.) (Capped)

DNA Binding Buffer (25 ml)

DNA Binding Buffer (50 ml)

DNA Wash Buffer (Concentrate) (24 ml)

DNA Wash Buffer (Concentrate) (48 ml)

DNA Wash Buffer (Concentrate) (6 ml)

DNA Binding Buffer (100 ml)

<h2>What are ELISA kits for methylation in this category?</h2>
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ELISA kits for DNA and RNA methylation in this category are antibody-based assays that quantify the global level of a specific epigenetic mark, most commonly 5-methylcytosine in genomic DNA, 5-hydroxymethylcytosine in genomic DNA, or N6-methyladenosine in RNA, using a plate-based immunoassay format without requiring bisulfite conversion or sequencing. Denatured, single-stranded DNA or RNA from a sample is coated on a plate well, and a mark-specific primary antibody followed by an HRP-conjugated secondary antibody generates a colorimetric signal proportional to the level of the target modification.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">5-mC DNA ELISA kits for quantitative analysis of DNA methylation&nbsp;</li>
<li dir="ltr" role="presentation">DNA controls for validation and calibration of the assay to ensure experimental&nbsp; reproducibility and accuracy&nbsp;</li>
<li dir="ltr" role="presentation">Secondary antibody reagents for specific signal generation and detection in ELISA assays</li>
<li dir="ltr" role="presentation">Coating buffers for consistent assay performance and optimized plate preparation&nbsp;</li>
</ul>
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<h2>How to choose methylation ELISA kits and reagents</h2>
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Match your kit to the specific epigenetic mark you're quantifying
Different kits use antibodies against specific modifications: 5-methylcytosine ELISA kits quantify global DNA methylation, 5-hmC ELISA kits quantify global hydroxymethylation, and m6A ELISA kits quantify RNA N6-methyladenosine. Using a kit with the wrong antibody specificity will not report the mark you intend to measure.
Use methylation standards to build a calibration curve for quantitative results
Running a series of methylation standards with defined percentages alongside your samples allows the absorbance readout to be converted to a specific, meaningful methylation percentage, since the raw ELISA signal alone cannot be interpreted as absolute methylation level without a calibration.
Understand that ELISA measures global levels, not locus-specific methylation
A methylation ELISA gives the overall average percentage of methylation across the genome in a sample, which is useful for comparing broad methylation changes between conditions but cannot identify which specific genes or regions are differentially methylated the way bisulfite sequencing can.
Confirm the required DNA denaturation step is appropriate for your sample type
ELISA kits for DNA methylation typically require single-stranded, denatured DNA coated directly on the plate, and confirming this denaturation step is properly performed is necessary for consistent antibody binding and reproducible signal across wells.
Be aware of cross-reactivity with similar modifications when interpreting results
An antibody against 5-methylcytosine may have some cross-reactivity with 5-hydroxymethylcytosine, and confirming the antibody's published cross-reactivity data helps interpret whether your signal specifically reflects 5-mC, 5-hmC, or both, which matters for studies in cell types where 5-hmC is abundant.
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<h2>Specifications context</h2>
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The 5-methylcytosine ELISA workflow uses an indirect ELISA methodology where denatured, single-stranded DNA samples are coated directly on the plate well surfaces and a 5-mC monoclonal antibody and an HRP-conjugated secondary antibody are added sequentially, generating a colorimetric signal proportional to the total level of methylated cytosine in the sample. As of 2026, methylation ELISA kits are particularly valued for high-throughput screening studies comparing global methylation levels across many treatment conditions or patient groups where single-base-resolution data is not the primary goal.
This category sits within DNA/RNA Methylation, alongside Methylation Standards for calibration, and is related to DNA Hydroxymethylation and Non-Bisulfite Kits &amp; Reagents for higher-resolution methylation analysis. See the full reagents catalog and use Quick Order for recurring orders.
<a href="https://mbpinc.net/contact-us">Contact the expert team of&nbsp; Molecular Biology Products Inc. (MBP)</a> to get high-quality ELISA Kits and Reagents for your lab.

ELISA Kits & Reagents

D5325-5-1-MSDS

D5325-5-2-MSDS

DNA Controls

DNA Controls are available in the following variants: D5325-5-1, D5325-5-2.DNA Controls D5325-5-1 by Zymo Research — Negative Control (0% 5-mC) for the 5-mC DNA ELISA Kit. E. coli genomic dsDNA at 100 ng/µl, 50 µl per unit, stored at −20°C. Used for standard curve construction and assay baseline in global DNA methylation quantification.