Zymo Reagents

Plates

ZymoPURE Filter Plate (2 pack)

C2022 by Zymo Research is a 2-pack of 96-well filter plates with 1.0 ml per well capacity for rapid clarification of bacterial lysates in high-throughput plasmid purification workflows. Refill component for the ZymoPURE 96 Plasmid Miniprep Kit (D4214/D4215); compatible with centrifuge and vacuum manifold.

Capacity

Dimensions (H x W x L)

Manufacturer

Shipping Condition

Storage Condition

<h2>What Are Zymo Research Buffers?</h2>
Zymo Research buffers are formulation-specific reagents used at defined steps &mdash; lysis, binding, wash, and elution &mdash; in Zymo-Spin column-based purification protocols for DNA, RNA, and bisulfite-converted DNA. Each buffer is optimized for a specific kit family: DNA Wash Buffer concentrate (requires ethanol addition before use) removes salts and contaminants before elution from Zymo-Spin silica columns; DNA Elution Buffer (10 mM Tris, pH 8.5, 0.1 mM EDTA) releases purified DNA from the matrix; RNA Wash Buffer supports RNA extraction kits including Quick-RNA and Direct-zol lines; M-Elution Buffer is designed for eluting bisulfite-converted DNA in EZ DNA Methylation kits. Researchers typically order these separately when a single buffer component runs out before the rest of the kit, or when scaling up a protocol beyond the volume included.
TL;DR: Order Zymo buffers individually to replenish a specific step in your existing Zymo workflow without replacing the full kit.
<h2>How to Choose Zymo Research Buffers</h2>
<h3>Match the buffer to your kit family</h3>
Every Zymo buffer is paired to a specific kit line. DNA Wash Buffer and DNA/RNA Wash Buffer concentrate are used in DNA Clean &amp; Concentrator, Quick-DNA, and Zymoclean Gel DNA Recovery kits. RNA Wash Buffer pairs with Quick-RNA kits. ZymoBIOMICS DNA Wash Buffer 1 and Wash Buffer 2 are specific to the ZymoBIOMICS microbiomics line. Substituting a buffer from a different kit family can compromise yield or purity.
<h3>Confirm ethanol addition requirements before use</h3>
Several Zymo wash buffer concentrates require addition of 96-100% ethanol to the bottle before first use &mdash; the label states the exact volume. DNA Wash Buffer concentrate, DNA/RNA Wash Buffer concentrate, and Urine DNA Wash Buffer concentrate all require this step. Pre-made (non-concentrate) versions such as RNA Wash Buffer are supplied ready to use.
<h3>Select the right volume for your throughput</h3>
Zymo buffers are available in sizes from 6 ml (single-experiment replenishment) up to 500 ml (Buffer P1 Red, for high-volume plasmid prep labs). For labs processing more than 50 samples per week, ordering the largest available size reduces per-sample cost and the frequency of reordering.
<h3>Choose elution buffer chemistry based on downstream application</h3>
DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA) is the standard for most PCR and sequencing applications. For applications sensitive to EDTA &mdash; such as some restriction enzyme digests or direct use with divalent cation-dependent enzymes &mdash; eluting in molecular-grade water or a Tris-only buffer is preferable. M-Elution Buffer is specifically formulated for bisulfite-converted DNA and should not be substituted with standard elution buffer in methylation workflows.
<h3>Verify research-use-only status</h3>
All Zymo Research buffers sold through MBP are for research use only (RUO). They are not cleared for in vitro diagnostic use. For clinical or diagnostic laboratory applications, confirm regulatory status with your institutional compliance officer before use.
<h2>Compare Zymo Buffer Types</h2>
<table>
<thead>
<tr>
<th>Buffer Type</th>
<th>Primary Application</th>
<th>Kit Family</th>
<th>Format Available</th>
</tr>
</thead>
<tbody>
<tr>
<td>DNA Wash Buffer (concentrate)</td>
<td>Salt removal before DNA elution</td>
<td>DNA Clean &amp; Concentrator, Quick-DNA</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA/RNA Wash Buffer (concentrate)</td>
<td>Salt removal for dual DNA/RNA preps</td>
<td>Quick-DNA/RNA kits</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA)</td>
<td>DNA release from silica column</td>
<td>All Zymo DNA purification</td>
<td>16 ml &ndash; available sizes vary</td>
</tr>
<tr>
<td>RNA Wash Buffer</td>
<td>RNA purification wash step</td>
<td>Quick-RNA kits</td>
<td>Ready-to-use</td>
</tr>
<tr>
<td>M-Elution Buffer</td>
<td>Elution of bisulfite-converted DNA</td>
<td>EZ DNA Methylation kit families</td>
<td>Small volume</td>
</tr>
<tr>
<td>ZymoBIOMICS DNA Wash Buffer 2</td>
<td>Metagenomic/microbiomic DNA cleanup</td>
<td>ZymoBIOMICS DNA kits</td>
<td>Sold separately</td>
</tr>
<tr>
<td>Buffer P1 Red</td>
<td>Alkaline lysis resuspension (plasmid prep)</td>
<td>Zyppy Plasmid Miniprep</td>
<td>10 ml &ndash; 500 ml</td>
</tr>
</tbody>
</table>
<h2>Specifications Context</h2>
Zymo wash buffer concentrates are supplied at a defined fold-concentration (commonly 4x or 6x) and require ethanol addition to reach working strength &mdash; the exact ratio is printed on the bottle and in the kit protocol. Elution buffer volumes for Zymo-Spin columns typically range from 6 to 100 &micro;l per elution; larger elution volumes improve total yield at the cost of DNA concentration. For DNA elution from Zymo-Spin columns, the protocol recommends a minimum 1-minute contact time between elution buffer and the silica matrix to maximize recovery of fragments 10 kb and above. As of 2025, Zymo Research tests all buffer lots for performance consistency, with integrity guaranteed for up to one year from purchase date.
Browse the full <a href="https://www.notion.so/reagents">Zymo Research reagents collection at MBP</a> for kits and columns that use these buffers, or visit our <a href="https://www.notion.so/reagents/buffers">reagent buffers category</a> for non-Zymo buffer options including ABM and other brands. For volume orders or purchase orders, use the <a href="https://www.notion.so/quickorder">Quick Order</a> tool or <a href="https://www.notion.so/contact-us">contact us</a> directly &mdash; MBP is a registered vendor for institutional procurement systems.
Last updated: June 2025
&nbsp;

Zymo Buffers

MSDS

ZymoPURE Wash 1 for ZymoPURE Plasmid Purification Kits

D4200-5 by Zymo Research is ZymoPURE Wash 1, the ready-to-use first wash buffer (5 ml per prep) applied after DNA binding in ZymoPURE II Midiprep, Maxiprep, and Gigaprep workflows. No ethanol preparation needed. Removes initial-pass protein and RNA contaminants before ZymoPURE Wash 2 (D4200-6). Available in 20–500 ml sizes.

ZymoPURE™ Wash 1 (20 ml)

ZymoPURE™ Wash 1 (320 ml)

ZymoPURE™ Wash 1 (410 ml)

ZymoPURE™ Wash Buffer 1 (500 ml)

ZymoPURE™ Wash 1 (55 ml)

ZymoPURE™ Elution Buffer for ZymoPURE Plasmid Purification Kits

Zymo Research D4200-7 ZymoPURE™ Elution Buffer: 10 mM Tris-HCl pH 8.5, 0.1 mM EDTA. Compatible with ZymoPURE Miniprep, ZymoPURE II Midiprep/Maxiprep/Gigaprep, and ZymoPURE-Express Midiprep kits. Available in 1 mL, 6 mL, 12 mL, and 30 mL volumes. Room temperature storage. Non-hazardous. Made in USA.

ZymoPURE™ Elution Buffer (1 ml)

ZymoPURE™ Elution Buffer (12 ml)

ZymoPURE™ Elution Buffer (30 ml)

ZymoPURE™ Elution Buffer (6 ml)

<h2>What Are Gigaprep Kits?</h2>
&nbsp;
Gigaprep kits are alkaline-lysis-based plasmid purification systems scaled for bacterial cultures of 1-2.5 L, yielding 10-25 mg of plasmid DNA per preparation. The purified DNA is endotoxin-free (typically &lt;=0.1 EU/ug), concentrated to up to 6 ug/ul, and compatible with demanding downstream workflows including mammalian cell transfection, in vitro transcription, gene therapy vector production, and automated sequencing. Unlike miniprep or maxiprep kits, a gigaprep processes sufficient DNA to support multi-well transfection screens, viral vector packaging batches, or long-running in vitro assays from a single prep.&nbsp;Choose a gigaprep kit when your protocol requires more than 2-5 mg of transfection-grade plasmid DNA in a single preparation.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="1q3vh4q" data-start="25" data-end="147">Large-scale plasmid DNA purification kits, including the ZymoPURE&trade; II Plasmid Gigaprep Kit, for high-yield DNA recovery.</li>
<li data-section-id="epfgfk" data-start="148" data-end="266">Gravity-flow purification systems that eliminate the need for vacuum manifolds and centrifugation during processing.</li>
<li data-section-id="hdtzmb" data-start="267" data-end="364">Advanced anion-exchange column technology for efficient removal of contaminants and impurities.</li>
<li data-section-id="1ti4gf8" data-start="365" data-end="467">Transfection-grade plasmid DNA preparation solutions suitable for demanding downstream applications.</li>
<li data-section-id="82zytf" data-start="468" data-end="598" data-is-last-node="">High-capacity plasmid isolation products designed for research, protein expression, and large-scale molecular biology workflows.</li>
</ul>
&nbsp;
<h2>How to Choose Gigaprep Kits</h2>
&nbsp;
Purification Chemistry
Anion-exchange resin kits (gravity-flow or vacuum column) use charged resin to bind nucleic acids and separate plasmid from chromosomal DNA, RNA, and endotoxin by salt gradient. Spin-column formats such as the ZymoPURE II Gigaprep use a silica-based membrane with specialized endotoxin-removal steps, completing the prep in ~45 minutes without gravity-flow wait times. Choose anion-exchange for the highest A260/A280 purity when supercoiling integrity is critical; choose spin-column when throughput and speed are the priority.
Endotoxin Removal Requirement
Endotoxin specification matters most for the transfection of primary cells, dendritic cells, or endotoxin-sensitive in vivo models. Kits rated at &lt;=0.1 EU/ug are considered endotoxin-free for most transfection applications. If you work with standard transformed cell lines (HEK293, CHO), standard endotoxin reduction is usually sufficient; for primary neurons, immune cells, or gene therapy studies, confirm the &lt;=0.1 EU/ug spec before selecting a kit.
Starting Culture Volume
Most gigaprep kits are optimized for 1.2-2.5 L of LB or terrific broth (TB). TB medium typically yields 30-50% more plasmid per liter than LB for high-copy vectors. Confirm whether your kit protocol is calibrated for LB or TB to ensure the binding column is not overloaded, which degrades purity.
Plasmid Copy Number
High-copy plasmids (&gt;100 copies/cell, pUC-based) approach theoretical yield limits easily; scale down culture volume if needed to avoid column overload. Low-copy plasmids (bacterial artificial chromosomes, BACs, PACs, YACs) require the full 2-2.5 L and benefit from kits specifically validated for low-copy applications.
Supercoiling Retention
Supercoiled plasmid is the biologically active isoform preferred for transfection and in vitro transcription. Validated gigaprep kits preserve &gt;85% supercoiled plasmid; avoid protocols with aggressive vortexing or extended lysis to protect this fraction.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Gigaprep eluates are typically quantified by A260 absorbance and evaluated for purity by A260/A280 ratio (target 1.8-2.0) and A260/A230 ratio (target &gt;1.8). Endotoxin is measured by LAL (Limulus amebocyte lysate) assay; a spec of &lt;=0.1 EU/ug is the accepted threshold for transfection-grade plasmid. As of 2025, the most widely adopted spin-column gigaprep format yields DNA concentrated to 4-6 ug/ul, reducing downstream volume requirements for transfection. All kits supplied through MBP are for Research Use Only (RUO) and are not classified as IVD or medical-device reagents.
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Gigaprep Kits

DataSheet

Protocol

ZymoPURE™ II Plasmid Gigaprep Kit (5 Preps.)

ZymoPURE™ II Plasmid Gigaprep Kit D4204 by Zymo Research purifies up to 25 mg of endotoxin-free (≤0.025 EU/µg), transfection-ready plasmid DNA from up to 2.5 L of E. coli culture in 45 minutes — no alcohol precipitation required. 5 preps per kit.

Applicable For

Binding Capacity

Culture Input

Elution Volume

Endotoxin Levels

Equipment

Format

Processing Time

Purity

Recommended Sample Volume

Size Range

Yield

<h2>What are plasmid maxiprep kits?</h2>
&nbsp;
A plasmid maxiprep kit scales SDS/alkaline lysis with silica-membrane spin columns, anion-exchange resin with gravity flow, or fully automated cartridge-based processing to 100-500 ml of overnight E. coli culture, recovering up to 400 ug per spin-column prep or 1-1.4 mg with high-yield anion-exchange chemistry, completable in 35-75 minutes depending on format. Related entities include EndoFree-type endotoxin removal for transfection, automated processors that eliminate manual centrifugation, and downstream applications including large-scale transfection, in vitro transcription/translation, and multiple parallel experiments. Choose Maxiprep when your application needs the largest routine plasmid yields (hundreds of micrograms to over 1 mg) from a single overnight culture.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Ultrafast 25-minute maxiprep kits drastically reduce processing times using specialized column technology&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Syringe-filter plasmid maxi units rapidly clear dense bacterial lysates using vacuum-free clarification systems&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Endotoxin-free plasmid maxiprep systems for delivering transfection-grade DNA in sensitive mammalian cell lines</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">High-performance silica-column vectors optimized for maximum concentration and recovery of low and high-copy plasmids</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Gravity-flow anion-exchange assemblies without manual centrifugation steps, ensuring chromatography-level purity&nbsp;</li>
</ul>
&nbsp;
<h2>How to choose a plasmid maxiprep kit</h2>
&nbsp;
When maxiprep is the right scale
A maxiprep should be used when purifying plasmid from an overnight bacterial culture with a volume between 100-500 ml, when downstream needs exceed midiprep's 0.5-2 mg range - for example, large-scale transfection across many plates, in vitro transcription/translation requiring substantial template DNA, or banking DNA for multiple future experiments from one culture. If midiprep yields already meet your needs, the larger culture volume and processing time of maxiprep may not be justified.
Spin-column vs. anion-exchange vs. automated chemistry
Spin-column maxipreps (e.g., 60-minute procedures) recover up to roughly 400 ug per prep, while anion-exchange or high-yield chemistries can reach 1-1.4 mg from comparable culture volumes; automated cartridge-based processors can deliver endotoxin-free DNA in under 75 minutes without manual centrifugation, pouring in culture, and pressing start. Match chemistry to both your yield target and available equipment - automated processors reduce hands-on time but represent a larger equipment investment.
Endotoxin levels at maxi scale
Endotoxin concentrations tend to be higher in larger-scale preparations - standard maxipreps have been reported at 11-16 EU/ug, while EndoFree-type kits target below 0.1 EU/ug through additional washing, and "substantially endotoxin-free" is sometimes defined as under 100 EU/ug for less stringent applications. For transfection of highly sensitive cells, vaccination research, or gene therapy applications, confirm the maxiprep kit's specific endotoxin target rather than assuming "maxiprep" implies low endotoxin by default.
Lysate clearing and centrifuge requirements
Maxi-scale lysate clearing typically requires high-speed centrifugation of the full culture volume (100-500 ml) followed by a clearing column or filtration step; gravity-flow anion-exchange systems use folded filters to avoid this centrifugation, while automated processors handle clearing internally. Confirm your centrifuge has rotors compatible with 100-500 ml culture volumes before committing to a centrifugation-based maxiprep format.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for maxiprep selection are expected yield (400 ug for spin-column vs. 1-1.4 mg for high-yield/anion-exchange chemistry), endotoxin target relative to your transfection application's sensitivity, and lysate-clearing method relative to your centrifuge's capacity for 100-500 ml culture volumes. A maxiprep should specifically be used for culture volumes in the 100-500 ml range; smaller volumes are better served by <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/midiprep-kits">midiprep kits</a>&nbsp;or&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/miniprep-kits">miniprep kits</a>. Labs running large-scale transfection or in vitro transcription/translation programs typically standardize on either high-yield anion-exchange maxipreps or automated processors to balance yield, endotoxin level, and hands-on time. The parent <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits">plasmid DNA extraction kits category</a>&nbsp;covers the full mini-to-maxi range, while the broader&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits">nucleic acid extraction kits hub</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits category</a>&nbsp;link to chromosomal DNA workflows. Use MBP's&nbsp;<a href="https://mbpinc.net/quickorder">Quick Order tool</a>&nbsp;for case-quantity pricing on maxiprep kits or&nbsp;<a href="https://mbpinc.net/contact-us">contact the specialist team</a>&nbsp;for guidance on endotoxin levels and automation options for your transfection program.
<section class="mbp-contact-cta"></section>
&nbsp;
Scale-up workflow efficiency with MBP&rsquo;s dependable macro-extraction plasmid technologies. <a href="https://mbpinc.net/contact-us">Contact the expert team</a> today to find the perfect match for your lab.

Maxiprep Kits

Data Sheet

ZymoPURE™ II Plasmid Maxiprep Kit

ZymoPURE™ II Plasmid Maxiprep Kit D4202 by Zymo Research purifies up to 3 mg of endotoxin-free (≤0.025 EU/µg), transfection-ready plasmid DNA from up to 150 mL of E. coli culture in ≤18 minutes, with A260/230 ≥2.0 purity. Supports plasmids up to 200 kb.

ZymoPURE™ II Plasmid Maxiprep Kit (10 Preps.)

ZymoPURE™ II Plasmid Maxiprep Kit (20 Preps.)

<h2>What are plasmid midiprep kits?</h2>
&nbsp;
A plasmid midiprep kit scales SDS/alkaline lysis and silica-membrane column binding (or anion-exchange resin with gravity flow) to 150-300 ml of overnight E. coli culture, recovering 0.5-2 mg of plasmid DNA with lysate clearing accomplished by high-speed centrifugation followed by a clearing column or filtration step, completable in as little as 30 minutes for some spin/vacuum formats. Related entities include high-speed centrifugation for lysate clearing, NucleoSnap-type midi formats, endotoxin removal washes, and downstream applications including moderate-scale transfection and multi-reaction restriction/sequencing work. Choose midiprep when miniprep yields (up to ~25-100 ug) aren't sufficient, but full maxiprep-scale culture volume (100-500 ml) and processing time aren't necessary.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Rapid 25-minute midiprep kits to bypass lengthy gravity-flow precipitation steps utilizing accelerated column dynamics</li>
<li dir="ltr" role="presentation">Vacuum-assisted syringe-filter plasmid midi modules engineered for rapid and debris-free clearing of lysates without manual pelleting</li>
<li dir="ltr" role="presentation">Endotoxin-free intermediate purification systems for sensitive mammalian cell screening by delivering transfection-grade constructs</li>
<li dir="ltr" role="presentation">High-purity silica spin-column configurations optimized for elution of high-copy vectors&nbsp;</li>
<li dir="ltr" role="presentation">Advanced anion-exchange gravity-flow assemblies provide template purity for downstream sequencing.</li>
</ul>
&nbsp;
<h2>How to choose a plasmid midiprep kit</h2>
&nbsp;
When midiprep fits between mini and maxi
Midiprep format is the right choice when your downstream application needs more DNA than a miniprep provides (roughly 20-100 ug depending on chemistry) but doesn't require the largest maxiprep-scale yields (up to 1 mg or more); 0.5-2 mg from 150-300 ml culture covers many moderate-scale transfection experiments and multi-replicate downstream reactions. If you find yourself running multiple minipreps to pool DNA for a single transfection, a single midiprep is often more efficient.
Lysate clearing method
Midiprep kits clear lysate using high-speed centrifugation followed by a clearing column (spin or vacuum format) or filtration through folded filters in anion-exchange systems; spin/vacuum clearing formats can complete the full protocol in as little as 30 minutes, while gravity-flow anion-exchange systems may take longer but avoid centrifugation steps for the binding/wash/elute portion. Match the clearing method to your lab's available centrifuge capacity (rotor size for 150-300 ml tubes) and time constraints.
Endotoxin-free midiprep options
For transfection of sensitive cell types, endotoxin-free midiprep kits using anion-exchange chemistry can achieve endotoxin removal in a unique washing step without additional incubations, suitable for transfection of highly sensitive cells, vaccination research, or gene therapy applications. If your midi-scale prep feeds directly into sensitive-cell transfection, confirm the kit's endotoxin specification (e.g., below 0.1 EU/ug) against your cell type's documented sensitivity.
Plasmid copy number and culture volume within the midi range
As with miniprep, plasmid copy number affects yield within the midiprep culture volume range - low-copy plasmids may need culture volumes toward the higher end (300 ml) to reach typical midi yields, while high-copy plasmids may reach 1-2 mg even at 150 ml. Plan culture volume within the midi range based on your specific vector rather than defaulting to the kit's maximum stated volume.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for midiprep selection are whether 0.5-2 mg from 150-300 ml culture meets your downstream DNA quantity needs without over- or under-scaling, lysate clearing method (centrifugation-based spin/vacuum vs. gravity-flow anion-exchange), and endotoxin status for transfection-sensitive applications. A midiprep completing in as little as 30 minutes via spin/vacuum clearing offers a time advantage over gravity-flow systems while still avoiding the larger culture volumes of maxiprep. Labs whose miniprep yields are consistently insufficient for their transfection protocols typically move to <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/midiprep-kits">midiprep kits</a>&nbsp;before considering&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/maxiprep-kits">maxiprep kits</a>, which require substantially more culture volume and processing time. The parent&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits">plasmid DNA extraction kits category</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/miniprep-kits">miniprep kits</a>&nbsp;cover smaller-scale options, while the broader&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits">nucleic acid extraction kits hub</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits category</a> link to chromosomal DNA workflows.
&nbsp;
<section class="mbp-contact-cta">
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Midiprep Kits

D4201 - MSDS

D4201 (D4200/D4201)

ZymoPURE™ II Plasmid Midiprep Kit

D4201 by Zymo Research: ZymoPURE™ II Plasmid Midiprep Kit, 50 preps. Processes ≤50 mL E. coli culture; yields 200–400 µg; ≤18 min protocol. Endotoxin ≤0.025 EU/µg (EndoZero II) or ≤1 EU/µg standard. Up to 200 kb; A260/A280 ≥1.8. Made in USA.

ZymoPURE™ II Plasmid Midiprep Kit (50 Preps.)

ZymoPURE™ II Plasmid Midiprep Kit (25 Preps.)

ZymoPURE™ P1 (Red) for ZymoPURE Plasmid Purification Kits

D4200-1 by Zymo Research is ZymoPURE™ P1 (Red), the RNase A-containing resuspension buffer for ZymoPURE and ZymoPURE II Plasmid Purification Kits (Miniprep, Midiprep D4200/D4201, Maxiprep D4202/D4203). The red color visually confirms complete bacterial pellet resuspension before alkaline lysis. Available in 3 ml to 410 ml sizes. Produces transfection-grade plasmid DNA from E. coli. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, Eurofins, and many other institutes across the USA.

ZymoPURE™ P1 (Red) (100 ml)

ZymoPURE™ P1 (Red) (13 ml)

ZymoPURE™ P1 (Red) (150 ml)

ZymoPURE™ P1 (Red) (210 ml)

ZymoPURE™ P1 (Red) (3 ml)

ZymoPURE™ P1 (Red) (410 ml)

ZymoPURE™ P2 (Blue) for ZymoPURE Plasmid Purification Kits

D4200-2 by Zymo Research is the ZymoPURE™ P2 (Blue) alkaline lysis buffer for ZymoPURE II Midiprep, Maxiprep, and Gigaprep plasmid purification kits. Contains NaOH; 8 mL used per midiprep (up to 50 mL culture). Available in multiple volumes.

ZymoPURE™ P2 (Blue) (100 ml)

ZymoPURE™ P2 (Blue) (13 ml)

ZymoPURE™ P2 (Blue) (150 ml)

ZymoPURE™ P2 (Blue) (210 ml)

ZymoPURE™ P2 (Blue) (3 ml)

ZymoPURE™ P2 (Blue) (410 ml)

ZymoPURE™ P3 (Yellow) for ZymoPURE Plasmid Purification Kits

The D4200-3 by Zymo Research is ZymoPURE P3, the yellow neutralization buffer for ZymoPURE Plasmid Purification Kits (Miniprep, Midiprep II, Maxiprep II). Used as Step 3 of the alkaline lysis workflow, P3 neutralizes the lysate and co-precipitates chromosomal DNA and proteins before column capture. Available in six sizes from 3 ml to 410 ml for high-volume replenishment.

ZymoPURE™ P3 (Yellow) (100 ml)

ZymoPURE™ P3 (Yellow) (13 ml)

ZymoPURE™ P3 (Yellow) (150 ml)

ZymoPURE™ P3 (Yellow) (210 ml)

ZymoPURE™ P3 (Yellow) (3 ml)

ZymoPURE™ P3 (Yellow) (410 ml)

<h2>What are plasmid miniprep kits?</h2>
&nbsp;
A plasmid miniprep kit uses SDS/alkaline lysis to break open pelleted E. coli cells from a small culture volume (1-10 ml), followed by binding to a silica-membrane spin column, washing, and elution, separating plasmid DNA from genomic DNA, RNA, and protein in roughly 10-15 minutes per prep. Related entities include Buffer P1/P2/P3-style alkaline lysis reagents, color-coded lysis dyes for visual confirmation of complete lysis and neutralization, RNase A for RNA removal, and downstream applications including transformation, restriction digestion, PCR, and sequencing. Choose a miniprep kit for routine, low-volume plasmid prep (cloning screens, sequencing checks, restriction digests) where yields up to roughly 20-100 ug per column are sufficient.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Pellet-free and classic plasmid miniprep kits for high-yield bacterial isolation using rapid spin-column workflows</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">High-throughput 96-well plasmid plates optimized for vacuum manifold processing and parallel automated screening&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Specialized yeast plasmid miniprep systems for fragile fungal hosts using cell-wall digesting chemistries</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">BAC/PAC and large construct isolation units for safer purification of high-molecular-weight vectors</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Endotoxin-free plasmid extraction reagents for sensitive mammalian cell lines, ensuring transfection-grade DNA purity&nbsp;</li>
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<h2>How to choose a plasmid miniprep kit</h2>
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Standard vs. high-yield chemistry
Standard silica spin-column minipreps typically recover up to about 20 ug per column, while high-yield formulations using modified alkaline lysis and novel binding chemistry can reach up to 100 ug at concentrations up to 3 ug/ul from the same small culture volume. If your downstream application (e.g., sequencing multiple samples from one prep, or transfection requiring more DNA per prep) benefits from a higher concentration, a high-yield kit reduces the need to scale up to midiprep.
Plasmid copy number and culture volume planning
High-copy plasmids (pUC-based vectors) can saturate a miniprep column from as little as 3 ml of culture, yielding around 20 ug, while low-copy plasmids (pBR322, pET, cosmids) may yield under 2 ug from the same volume and benefit from doubling culture volume to 5-10 ml. Identify your vector's origin of replication before choosing a standard culture volume, since low-copy vectors at standard volumes can produce yields too low for some downstream steps.
Color-coded lysis buffers for visual QC
Some miniprep kits include color-coded lysis and neutralization buffers that allow visual confirmation of complete cell lysis (color change) and proper neutralization before loading the column, helping catch incomplete-lysis failures before they waste a column. This visual QC step is particularly useful for new users or when troubleshooting inconsistent yields across a batch of preps.
Endotoxin-free and large-construct options
For transfection-sensitive downstream applications, endotoxin-free miniprep kits add a dedicated wash step without significantly extending the protocol time; for large plasmids, cosmids, or BAC/YAC/PAC constructs, some miniprep kits are validated up to approximately 200 kb, while standard kits are optimized for constructs up to roughly 25 kb. Confirm both endotoxin status and size compatibility match your specific construct and downstream use before relying on a standard miniprep.
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<h2>Specifications context</h2>
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The key specs for plasmid miniprep selection are expected yield relative to your plasmid's copy number and culture volume, whether color-coded buffers aid visual QC, and whether endotoxin-free or large-construct compatibility is needed for your downstream application. A standard miniprep yielding up to roughly 20-25 ug in 10-15 minutes from 1-10 ml of culture covers most routine cloning workflows, while high-yield kits up to 100 ug reduce the need to scale up for moderately DNA-hungry applications. Labs running both daily cloning screens and occasional larger-scale prep work typically keep <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/miniprep-kits">miniprep kits</a>&nbsp;on hand alongside&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/midiprep-kits">midiprep kits</a>&nbsp;for when miniprep yields aren't sufficient. The parent&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits">plasmid DNA extraction kits category</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/maxiprep-kits">maxiprep kits</a>&nbsp;cover larger-scale options, while the broader&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits">nucleic acid extraction kits hub</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits category</a> link to chromosomal DNA workflows.&nbsp;
<section class="mbp-contact-cta">
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MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Miniprep Kits

MSDS - D4208T

MSDS - D4209/D4210/D4211/D4212

ZymoPURE™ Plasmid Miniprep Kit

D4208T ZymoPURE Plasmid Miniprep Kit by Zymo Research — 10 preps; 15-min protocol; up to 100 &micro;g yield; &le;1 EU/&micro;g endotoxin; 25 &micro;l elution; A260/A280 &ge;1.8; plasmids to ~200 kb.

Sample Volume

ZymoPURE™ Plasmid Miniprep Kit (10 Preps.)

ZymoPURE™ Plasmid Miniprep Kit (50 Preps.)

ZymoPURE™ Plasmid Miniprep Kit (100 Preps.)

ZymoPURE™ Plasmid Miniprep Kit (400 Preps.)

ZymoPURE Plasmid Miniprep Kit (800 Preps.)

Syringe-Filters

ZymoPURE™ Syringe Filters (5 Pack)

C1036-5 by Zymo Research provides five ZymoPURE Syringe Filters for rapid clarification of up to 60 ml of neutralized bacterial lysate per filter in large-scale plasmid purification workflows. Polypropylene construction with a silicone-lubricated plunger and pre-attached ABS Luer-Lock plug; non-sterile, supplied in a pack of 5. Designed for use with ZymoPURE II Plasmid Midiprep, Maxiprep, and Gigaprep kits. Room temperature storage.

Matrix/Construction

Volumetric Capacity

Columns, Plastics & Accessories

ZymoPURE™ Syringe Plungers (5 Pack)

C1037-5 by Zymo Research is a 5-pack of replacement syringe plungers for ZymoPURE™ Midi and Maxiprep Plasmid Purification Kits. Supports isolation of up to 1.2 mg transfection-grade plasmid DNA from E. coli.

United States

Texas

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