Name: Zymo-Seq RiboFree™ Universal cDNA Kit (12 Preps.)
SKU: R3001
Availability: InStock

<h2>What Are Total RNA Isolation Kits?</h2>
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Total RNA isolation kits are complete reagent systems that capture all RNA species present in a biological sample - including messenger RNA (mRNA), ribosomal RNA (rRNA), transfer RNA (tRNA), long non-coding RNA (lncRNA), and small non-coding RNAs including microRNA (miRNA &gt;=17 nt) - in a single purification pass. This distinguishes them from mRNA-only kits (which use oligo-dT capture to select polyadenylated transcripts) and from small RNA kits (which use size fractionation to enrich miRNA and siRNA). Standard mini-format kits process samples of 5-100 mg tissue or 1x10^4-1x10^7 cells and yield 1-100 ug of total RNA with A260/A280 ratios of 1.8-2.1 and RIN scores &gt;=8 from fresh or properly snap-frozen material.&nbsp;Total RNA kits are the correct choice for any workflow where the complete transcriptome is needed - RNA-seq, whole-transcriptome microarray, or small RNA co-profiling - rather than a specific RNA fraction.
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<h2 dir="ltr">What you will find:</h2>
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<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/rna-extraction-kits/total-rna/spin-column">Spin-column:</a> for rapid, centrifugal transcript purification utilizing advanced silica membranes&nbsp;
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<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/rna-extraction-kits/total-rna/96-well-plate">96-well plate:</a> for parallel and high-throughput isolation of&nbsp;cellular RNA
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<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/rna-extraction-kits/total-rna/mag-beads">Mag Beads: </a>for flexible and automated transcript recovery using superparamagnetic particles
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<h2>How to Choose Total RNA Isolation Kits</h2>
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Sample Type and Lysis Method
Cultured cells and soft tissues (liver, brain, kidney) lyse efficiently in standard guanidinium isothiocyanate (GITC) or proprietary lysis buffers; no additional disruption is required. Fibrous tissues (skeletal muscle, heart, cartilage, plant material) require mechanical disruption via bead mill homogenizer (e.g., TissueLyser, BeadBug) or rotor-stator before the lysis buffer contacts the sample. Bacteria and yeast require specialized lysis conditions including enzymatic cell-wall digestion or bead beating at higher intensity. FFPE tissue sections need deparaffinization with xylene or substitute and extended proteinase K digestion; select kits explicitly validated for FFPE.
Small RNA Co-Purification
Total RNA kits differ in their ability to retain small RNA species (17-200 nt). Kits with a unique binding buffer chemistry - such as Zymo Research Quick-RNA kits - capture miRNA alongside large RNA in the same column pass. Conventional silica-column kits with high-ethanol wash steps often wash away RNA species under 200 nt. If your experiment requires miRNA detection (RT-qPCR for specific miRNAs, or small RNA sequencing), confirm the kit's small RNA retention profile before ordering.
DNase Digestion
On-column DNase I digestion is included with most total RNA kits and removes genomic DNA contamination during the wash stage. If the kit does not include DNase, a separate in-solution DNase I treatment (15-30 min at 37C) must follow elution for gene expression applications where gDNA amplification would interfere. Include on-column DNase kits for RT-qPCR and RNA-seq; the treatment adds fewer than 15 minutes to the protocol.
Binding Capacity and Sample Scale
Mini-format kits bind up to 100 ug total RNA and are adequate for most cell and tissue inputs. Midi or maxi kits are available for large tissue samples (&gt;100 mg). For micro-scale inputs (single cells, laser-capture microdissection sections, needle biopsies), use micro-format kits with 10-30 uL elution volumes to maximize concentration.
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<h2>Specifications Context</h2>
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Total RNA integrity is expressed as RIN (RNA integrity number, 1-10 scale; measured on Agilent Bioanalyzer 2100 or 4150 TapeStation). RNA-seq and microarray applications typically require RIN &gt;=7; single-cell or FFPE workflows often proceed with RIN 3-6 using short-amplicon RT-qPCR assays. Purity thresholds: A260/A280 1.8-2.1; A260/A230 &gt;1.8. Zymo Research Quick-RNA kits are among the most frequently cited total RNA isolation kits in published molecular biology methods.
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MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
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Total RNA

Zymo-Seq RiboFree™ Universal cDNA Kit (12 Preps.)

Zymo-Seq RiboFree™ Universal cDNA Kit (R3001) by Zymo Research prepares NGS-grade rRNA-depleted cDNA from 10–1,000 ng total RNA in 1.75 hours using probe-free universal depletion that works across mammalian, plant, bacterial, yeast, and FFPE samples. Outputs single-stranded cDNA ready for any compatible library prep method. 12-prep format (R3001), with Zymo-Seq Wash Buffer also sold separately in 6 mL (R3004-1-6) and 48 mL (R3004-1-48) sizes.