Name: Zymo-Seq RiboFree™ Total RNA Library Prep Kit
SKU: R3000
Availability: InStock

<h2>What are Magnetic Bead Total RNA Extraction Kits?</h2>
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Magnetic bead total RNA extraction kits isolate total RNA by adsorbing nucleic acids onto paramagnetic silica-coated beads in a high-chaotropic-salt lysis buffer, then using a magnetic field to capture and wash beads without centrifugation. Unlike standard spin-column kits, the bead format removes centrifuge dependency, enabling scalable manual processing in 96-well plates or full automation on liquid-handling robots.&nbsp;Choose a magnetic bead format when your lab processes more than 48 samples per day or when a Tecan, Hamilton, or Beckman Biomek platform is available.
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<h2 dir="ltr">What you will find:</h2>
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Magbio HighPrep Kits for liquid biopsy collection systems and non-invasive swabbing using rapid magnetic bead isolation&nbsp;
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Zymo-Seq RiboFree&trade; Kits to eliminate ribosomal RNA carryover during library synthesis
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Direct-zol-96 MagBead RNA Modules facilitate complete avoidance of manual phase-separation steps while enabling automated transcript extraction directly from TRIzol&reg; lysates&nbsp;
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Magbio HighPrep Total RNA Plus Kit extracts pure, inhibitor-free RNA from complex samples with maximum binding capacity&nbsp;
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Quick-RNA MagBead Packs deliver high-integrity total transcripts without dependence on centrifuge spins or vacuum manifold.
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<h2>How to Choose a Magnetic Bead Total RNA Extraction Kit</h2>
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Automation Compatibility
Confirm that the kit supplier provides automation scripts for your specific platform. The Zymo Research Quick-DNA/RNA Viral MagBead kit and Thermo Fisher MagMAX-96 both offer downloadable instrument scripts for Tecan and Hamilton systems. Generic magnetic bead kits without published automation protocols require in-house script development, adding significant setup time.
Small RNA Retention
Bead kits that bind RNA &gt;=17 nt co-purify microRNAs alongside mRNA and rRNA from the same input. Kits specifying &gt;=200 nt retention exclude small RNAs and are not appropriate for miRNA expression panels. Check the size cutoff stated in the kit datasheet before selecting for non-coding RNA work.
Input Sample Type
Magnetic bead kits for total RNA support cell pellets, tissue homogenates, blood, bacteria, yeast, and fungi. For cell-free matrices (plasma, serum, nasal wash), switch to a viral-RNA or liquid-biopsy magnetic bead kit with included carrier RNA to maximize recovery from low-input, cell-free samples.
Elution Volume and Concentration
Standard magnetic bead kits elute into 50-100 ul; some protocols allow concentration into 20-50 ul, increasing RNA concentration for sensitive downstream applications. If downstream libraries require &gt;=200 ng/ul input, reduce elution volume accordingly and verify the kit supports this protocol variant.
gDNA Removal
Confirm whether the kit includes on-bead DNase I or requires a separate gDNA digestion step. For RT-qPCR and RNA-seq, omitting DNase treatment introduces a genomic DNA signal that inflates expression measurements.
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<h2>Specifications Context</h2>
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RNA purity targets from magnetic bead extractions match spin-column benchmarks: A260/A280 &gt;= 1.8 and A260/A230 &gt;= 1.5. RNA integrity number (RIN) &gt;= 7 is recommended for RNA-seq library preparation; magnetic bead kits produce RIN values in the 8-10 range for fresh samples when lysis is performed immediately on ice. Bead-based workflows are cited in published automation protocols from genomics core facilities and are increasingly specified by NIH-funded projects requiring standardized sample processing across multi-site studies.
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP Inc. to get automation-compatible mag bead selections today.

Mag Beads

MSDS

R3000-MSDS

DataSheet

R3000-Protocol

Zymo-Seq RiboFree™ Total RNA Library Prep Kit

R3000 by Zymo Research is a total RNA library prep kit combining probe-free RiboFree™ rRNA depletion with stranded library construction in approximately 4 hours from 10–250 ng input. The enzymatic depletion requires no species-specific probes. Academic researchers running multi-species transcriptomics or FFPE RNA-seq benefit from validated protocols covering human, mouse, rat, E. coli, Arabidopsis, and low-input samples down to 1 ng.

Zymo-Seq RiboFree™ Total RNA Library Prep Kit

SKU : R3000