Zymo Reagents

<h2>What Are Zymo Research Buffers?</h2>
<p>Zymo Research buffers are formulation-specific reagents used at defined steps &mdash; lysis, binding, wash, and elution &mdash; in Zymo-Spin column-based purification protocols for DNA, RNA, and bisulfite-converted DNA. Each buffer is optimized for a specific kit family: DNA Wash Buffer concentrate (requires ethanol addition before use) removes salts and contaminants before elution from Zymo-Spin silica columns; DNA Elution Buffer (10 mM Tris, pH 8.5, 0.1 mM EDTA) releases purified DNA from the matrix; RNA Wash Buffer supports RNA extraction kits including Quick-RNA and Direct-zol lines; M-Elution Buffer is designed for eluting bisulfite-converted DNA in EZ DNA Methylation kits. Researchers typically order these separately when a single buffer component runs out before the rest of the kit, or when scaling up a protocol beyond the volume included.</p>
<p><strong>TL;DR:</strong> Order Zymo buffers individually to replenish a specific step in your existing Zymo workflow without replacing the full kit.</p>
<h2>How to Choose Zymo Research Buffers</h2>
<h3>Match the buffer to your kit family</h3>
<p>Every Zymo buffer is paired to a specific kit line. DNA Wash Buffer and DNA/RNA Wash Buffer concentrate are used in DNA Clean &amp; Concentrator, Quick-DNA, and Zymoclean Gel DNA Recovery kits. RNA Wash Buffer pairs with Quick-RNA kits. ZymoBIOMICS DNA Wash Buffer 1 and Wash Buffer 2 are specific to the ZymoBIOMICS microbiomics line. Substituting a buffer from a different kit family can compromise yield or purity.</p>
<h3>Confirm ethanol addition requirements before use</h3>
<p>Several Zymo wash buffer concentrates require addition of 96-100% ethanol to the bottle before first use &mdash; the label states the exact volume. DNA Wash Buffer concentrate, DNA/RNA Wash Buffer concentrate, and Urine DNA Wash Buffer concentrate all require this step. Pre-made (non-concentrate) versions such as RNA Wash Buffer are supplied ready to use.</p>
<h3>Select the right volume for your throughput</h3>
<p>Zymo buffers are available in sizes from 6 ml (single-experiment replenishment) up to 500 ml (Buffer P1 Red, for high-volume plasmid prep labs). For labs processing more than 50 samples per week, ordering the largest available size reduces per-sample cost and the frequency of reordering.</p>
<h3>Choose elution buffer chemistry based on downstream application</h3>
<p>DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA) is the standard for most PCR and sequencing applications. For applications sensitive to EDTA &mdash; such as some restriction enzyme digests or direct use with divalent cation-dependent enzymes &mdash; eluting in molecular-grade water or a Tris-only buffer is preferable. M-Elution Buffer is specifically formulated for bisulfite-converted DNA and should not be substituted with standard elution buffer in methylation workflows.</p>
<h3>Verify research-use-only status</h3>
<p>All Zymo Research buffers sold through MBP are for research use only (RUO). They are not cleared for in vitro diagnostic use. For clinical or diagnostic laboratory applications, confirm regulatory status with your institutional compliance officer before use.</p>
<h2>Compare Zymo Buffer Types</h2>
<table>
<thead>
<tr>
<th>Buffer Type</th>
<th>Primary Application</th>
<th>Kit Family</th>
<th>Format Available</th>
</tr>
</thead>
<tbody>
<tr>
<td>DNA Wash Buffer (concentrate)</td>
<td>Salt removal before DNA elution</td>
<td>DNA Clean &amp; Concentrator, Quick-DNA</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA/RNA Wash Buffer (concentrate)</td>
<td>Salt removal for dual DNA/RNA preps</td>
<td>Quick-DNA/RNA kits</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA)</td>
<td>DNA release from silica column</td>
<td>All Zymo DNA purification</td>
<td>16 ml &ndash; available sizes vary</td>
</tr>
<tr>
<td>RNA Wash Buffer</td>
<td>RNA purification wash step</td>
<td>Quick-RNA kits</td>
<td>Ready-to-use</td>
</tr>
<tr>
<td>M-Elution Buffer</td>
<td>Elution of bisulfite-converted DNA</td>
<td>EZ DNA Methylation kit families</td>
<td>Small volume</td>
</tr>
<tr>
<td>ZymoBIOMICS DNA Wash Buffer 2</td>
<td>Metagenomic/microbiomic DNA cleanup</td>
<td>ZymoBIOMICS DNA kits</td>
<td>Sold separately</td>
</tr>
<tr>
<td>Buffer P1 Red</td>
<td>Alkaline lysis resuspension (plasmid prep)</td>
<td>Zyppy Plasmid Miniprep</td>
<td>10 ml &ndash; 500 ml</td>
</tr>
</tbody>
</table>
<h2>Specifications Context</h2>
<p>Zymo wash buffer concentrates are supplied at a defined fold-concentration (commonly 4x or 6x) and require ethanol addition to reach working strength &mdash; the exact ratio is printed on the bottle and in the kit protocol. Elution buffer volumes for Zymo-Spin columns typically range from 6 to 100 &micro;l per elution; larger elution volumes improve total yield at the cost of DNA concentration. For DNA elution from Zymo-Spin columns, the protocol recommends a minimum 1-minute contact time between elution buffer and the silica matrix to maximize recovery of fragments 10 kb and above. As of 2025, Zymo Research tests all buffer lots for performance consistency, with integrity guaranteed for up to one year from purchase date.</p>
<p>Browse the full <a href="https://www.notion.so/reagents">Zymo Research reagents collection at MBP</a> for kits and columns that use these buffers, or visit our <a href="https://www.notion.so/reagents/buffers">reagent buffers category</a> for non-Zymo buffer options including ABM and other brands. For volume orders or purchase orders, use the <a href="https://www.notion.so/quickorder">Quick Order</a> tool or <a href="https://www.notion.so/contact-us">contact us</a> directly &mdash; MBP is a registered vendor for institutional procurement systems.</p>
<p><em>Last updated: June 2025</em></p>
<p>&nbsp;</p>

Zymo Buffers

MSDS

Viral RNA Buffer

<p>R1034-1-100 by Zymo Research is a 100 ml Viral RNA Buffer that facilitates viral particle lysis and RNA capture from biological liquids including plasma, serum, urine, whole blood, and saliva. Sold as a standalone replenishment SKU for Zymo Research viral RNA purification workflows. Lab managers running high-volume viral RNA isolation can reorder this component independently without purchasing a full kit replacement.</p>

Manufacturer

Shipping Condition

Storage Condition

Viral RNA Buffer (100 ml)

Viral RNA Buffer (50 ml)

Viral Wash Buffer (concentrate)

<p>R1034-2-24 by Zymo Research is the Viral Wash Buffer (concentrate) in a 24 ml format, a component reagent used in the wash step of Zymo Research viral RNA and DNA/RNA extraction workflows. The concentrate is prepared by adding ethanol per the relevant kit protocol and is stored at room temperature (15–30&#176;C). Available in 6, 24, and 48 ml concentrate sizes for flexible kit replenishment.</p>

Viral RNA Wash Buffer (Concentrate) (24 ml)

Viral Wash Buffer (Concentrate) (48 ml)

Viral Wash Buffer (Concentrate) (6 ml)

Plates

Wash Plate (2 pack)

<p>C2024 by Zymo Research is a 2-pack of 96-well polypropylene wash plates with 1.3 ml per well capacity, designed to minimize cross-contamination during ZymoPURE 96 Plasmid Miniprep workflows. Refill component for the ZymoPURE 96 Plasmid Miniprep Kit (D4214/D4215); compatible with centrifuge and vacuum manifold protocols.</p>

Capacity

Dimensions (H x W x L)

<h2 data-section-id="v1drft" data-start="0" data-end="43">What are cloning chemicals and reagents?</h2>
<p>&nbsp;</p>
<p data-start="45" data-end="864">Cloning chemicals and reagents cover the enzymes, buffers, and molecular biology-grade chemicals used in DNA fragment preparation, modification, and purification within a cloning workflow, including restriction endonucleases, T4 DNA ligase and other DNA-modifying enzymes (phosphatases, polymerases), reaction buffers, and plasmid extraction reagents. Related entities include restriction enzyme digestion, DNA dephosphorylation/phosphorylation, plasmid DNA purification (column-based and magnetic bead-based), and enzyme unit definitions.</p>
<p dir="ltr">&nbsp;</p>
<h2 dir="ltr"><span style="font-family: helvetica, arial, sans-serif;">What you will find:</span></h2>
<p dir="ltr">&nbsp;</p>
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation"><span style="font-family: arial, helvetica, sans-serif;">Targeted XJa and XJb strains for faster recovery of proteins.</span></li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation"><span style="font-family: arial, helvetica, sans-serif;">Mix &amp; Go! solutions for optimized and dependable bacterial transformation.</span></li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation"><span style="font-family: arial, helvetica, sans-serif;">Culture Media &amp; Supplements used for yeast research, and include YPD Plus and mating pheromones.</span></li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation"><span style="font-family: arial, helvetica, sans-serif;">Extraction and Lysis Reagents include reliable genomic preparation and yeast protein kits.</span></li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation"><span style="font-family: arial, helvetica, sans-serif;">Chromogenic Substrates and Enzymatic Inducers with highly pure X-GAL and IPTG for cloning and blue-white screening.</span></li>
</ul>
<p dir="ltr">&nbsp;</p>
<h2 data-section-id="1daajgc" data-start="866" data-end="913">How to choose cloning chemicals and reagents</h2>
<p>&nbsp;</p>
<p data-section-id="wwlbzr" data-start="915" data-end="972"><strong>Restriction enzyme selection and buffer compatibility</strong></p>
<p data-start="973" data-end="1289">Restriction enzymes require specific reaction buffers, and many modern enzyme lines use a single universal buffer compatible across multiple enzymes to simplify double-digest reactions; confirm buffer compatibility when combining enzymes from different suppliers or using older single-buffer-per-enzyme formulations.</p>
<p data-section-id="1ezf0zg" data-start="1291" data-end="1337"><strong>Enzyme unit definitions and reaction scale</strong></p>
<p data-start="1338" data-end="1697">Restriction enzyme and ligase activity is defined in units per microliter, with one unit typically defined as the amount needed to digest or ligate a defined quantity of standard substrate DNA under specified conditions in a set time; scale enzyme quantity to the amount of DNA being processed rather than assuming a fixed volume works for all reaction sizes.</p>
<p data-section-id="11rzr02" data-start="1699" data-end="1729"><strong>Plasmid purification grade</strong></p>
<p data-start="1730" data-end="2069">Plasmid DNA extraction reagents range from molecular biology grade, suitable for general cloning and sequencing, to transfection-grade or endotoxin-free formulations required for applications such as lentiviral packaging or mammalian cell transfection, where endotoxin contamination can reduce transfection efficiency or cause cell stress.</p>
<p data-section-id="1acrdv0" data-start="2071" data-end="2128"><strong>Dephosphorylation for preventing vector self-ligation</strong></p>
<p data-start="2129" data-end="2455">Alkaline phosphatase treatment of a linearized vector before ligation removes 5' phosphate groups, preventing vector self-ligation and improving the ratio of recombinant clones; this step is particularly useful when the vector and insert share compatible sticky ends that could otherwise religate without insert incorporation.</p>
<p data-start="2506" data-end="3457">&nbsp;</p>
<h2 data-section-id="1fljvas" data-start="3459" data-end="3484">Specifications context</h2>
<p>&nbsp;</p>
<p data-start="3486" data-end="4215">Restriction enzymes and ligases are supplied at defined unit concentrations (commonly 10&ndash;20 units per microliter for restriction enzymes), with reaction buffers formulated for specific pH and salt conditions optimized for enzyme activity. Plasmid purification kits are differentiated by yield (micrograms of DNA recovered), purity (A260/A280 ratio), and endotoxin level (endotoxin units per microgram), with endotoxin-free kits specifying levels below a defined threshold suitable for transfection applications.&nbsp;</p>
<p data-start="4217" data-end="4952">This sub-category is part of MBP's cloning catalog alongside ligation independent cloning kits and transformation kits and competent cells, used downstream of enzyme-based fragment preparation. Labs producing custom lentivirus for immortalization can also review MBP's cell immortalization catalog. MBP's specialist team can help confirm enzyme buffer compatibility and plasmid purification grade for a specific downstream application before order placement.</p>
<p dir="ltr"><span style="font-family: arial, helvetica, sans-serif;"><a href="https://mbpinc.net/contact-us"><span style="text-decoration: underline; color: #236fa1;"><strong>Contact the expert team </strong></span></a>at MBP today to request a quote for high-purity chemicals and reagents.</span></p>

Chemicals/Reagents

X-GAL (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside)

<p>X1001-25 by Zymo Research is X-GAL, a ready-to-use 2% w/v sterile solution in DMF for blue-white colony screening and β-galactosidase detection. Available as X1001-25 (5 × 5 ml) and X1001-5 (1 × 5 ml). Formulated to minimize false positives. Store at -20°C. Made in the USA by Zymo Research.</p>

Concentration

Product Storage

5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-GAL) (5 x 5 ml)

5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-GAL) (5 ml)

DataSheet

Protocol

XJa (DE3) Autolysis Glycerol Stock (1 Tube)

<p>XJa (DE3) Autolysis Glycerol Stock T5031 by Zymo Research — 1 mL glycerol stock with 500× L-arabinose — provides 80–90% cell lysis after a single freeze-thaw cycle (~10 min post-harvest). The DE3 lysogen supports T7 promoter–based recombinant protein expression; growth requires 1 mM Mg²⁺ supplementation. Storage at –70°C to –80°C. Order via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or contact <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.</p>

Autolysis

Cell Growth

DNA Extraction

DNA Stability

Genotype

Processing Time

Protein Expression

XJa Autolysis Glycerol Stock (1 Tube)

<p>T5021 by Zymo Research is the XJa Autolysis Glycerol Stock — an EndA<sup>-</sup>/RecA<sup>-</sup> E. coli expression strain with L-Arabinose-inducible autolysis achieving 80–90% cell lysis in 10 minutes. Supplied as 1 tube with 1 ml 500× L-Arabinose. Handles plasmids up to 10 kb. Store at -70°C to -80°C. For protein expression and high-quality nucleic acid extraction.</p>

XJb (DE3) Autolysis Glycerol Stock (1 Tube)

<p>T5051 by Zymo Research is the XJb (DE3) Autolysis Glycerol Stock (1 ml, 1 tube), a self-lysing <em>E. coli</em> expression strain with DE3 T7 RNA polymerase, Lon⁻ OmpT⁻ background, and arabinose-inducible λ endolysin for 80–90% cell lysis within 10 minutes. Eliminates sonication in recombinant protein workflows. Supplied with 500× L-Arabinose. Store at -70°C to -80°C.</p>

XJb Autolysis Glycerol Stock (1 Tube)

<p>XJb Autolysis Glycerol Stock (T5041) by Zymo Research — arabinose-inducible autolysis E. coli strain for reagent-free recombinant protein extraction. Lon⁻, OmpT⁻. 80–90% lysis efficiency in ~10 min. 1 Tube, store at −70°C to −80°C.</p>

Y-Lysis Buffer

<p>Y-Lysis Buffer by Zymo Research is a specialized buffer for enzymatic yeast and fungal cell wall digestion, used at 25 µl per sample alongside Zymolyase in nucleic acid isolation workflows optimized for Saccharomyces cerevisiae and Candida albicans. Available in 6 ml and 100 ml formats.</p>

Y-Lysis Buffer (100 ml)

Y-Lysis Buffer (6 ml)

YPD Plus

<p>Y1003 by Zymo Research is a specially formulated YPD outgrowth medium that increases yeast transformation efficiency by more than 50% compared to standard YPD. Recommended for mutant strains with poor growth characteristics, library screening, and multi-plasmid transformation experiments. Available in 50 ml (Y1003-50) and 100 ml (Y1003-100) formats.</p>

YPD Plus (100 ml)

YPD Plus (50 ml)

YR Digestion Buffer (3.2 ml)

<p>R1001-1 by Zymo Research is a 3.2 mL YR Digestion Buffer for the YeaStar RNA Kit workflow, enabling enzymatic fungal cell wall digestion before RNA isolation. Stored at room temperature, non-hazardous, and available as a standalone replacement component.</p>

YR Lysis Buffer (6.4 ml)

<p>YR Lysis Buffer R1001-2 by Zymo Research is a 6.4 mL guanidinium thiocyanate-based lysis reagent for total RNA isolation from yeast and fungal species including Saccharomyces cerevisiae, Candida albicans, and Aspergillus fumigatus. Used with the YeaStar RNA Kit (R1002); provides approximately 40 preparations per bottle. Sold as a standalone replacement component.</p>