Zymo Reagents

<h2>What are Spin-Column Bacterial and Fungal RNA Extraction Kits?</h2>
<p>&nbsp;</p>
<p>Spin-column bacterial and fungal RNA extraction kits isolate total RNA from Gram-positive bacteria, Gram-negative bacteria, yeasts, filamentous fungi, algae, and protozoa using ultra-high-density BashingBead mechanical cell wall disruption in a chaotropic lysis buffer, followed by RNA binding to a Zymo-Spin silica-membrane column, on-column washing, and elution in 6-100 ul of RNase-free water - all using a standard microcentrifuge. The complete protocol takes under 15 minutes for a batch of 1-12 samples, requiring only a vortex with a microcentrifuge tube adapter, a microcentrifuge, and standard pipetting equipment. No specialized robotics, vacuum manifolds, or magnetic separation racks are needed. Choose a spin-column bacterial/fungal RNA kit for manual extraction of 1-24 samples per run, when existing automation infrastructure does not support magnetic bead formats, or when the fastest possible turnaround from culture to RT-qPCR data is required.</p>
<p>&nbsp;</p>
<h2 dir="ltr"><span style="font-family: arial, helvetica, sans-serif;">What you will find:</span></h2>
<p dir="ltr">&nbsp;</p>
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
<p dir="ltr" role="presentation"><span style="font-family: arial, helvetica, sans-serif;">Pinpoint&trade; Slide RNA Isolation System for targeted extraction of total RNA directly from localized tissue sections on microscope slides</span></p>
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
<p dir="ltr" role="presentation"><span style="font-family: arial, helvetica, sans-serif;">Quick-RNA&trade; Fungal/Bacterial MicroPrep Kit for highly concentrated, low-volume recovery of transcripts utilizing micro-elution spin-column matrices&nbsp;</span></p>
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
<p dir="ltr" role="presentation"><span style="font-family: arial, helvetica, sans-serif;">ZR Fungal/Bacterial RNA MiniPrep for precise cell-wall shattering of yeast and recalcitrant spores&nbsp;</span></p>
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
<p dir="ltr" role="presentation"><span style="font-family: arial, helvetica, sans-serif;">YeaStar&trade; RNA Kit efficiently permeabilizes tough yeast cell walls.&nbsp;</span></p>
</li>
</ul>
<p>&nbsp;</p>
<h2>How to Choose a Spin-Column Bacterial/Fungal RNA Kit</h2>
<p>&nbsp;</p>
<p><strong>Miniprep vs. Microprep Format</strong></p>
<p>The Zymo Quick-RNA Fungal/Bacterial Miniprep processes up to 2x10^8 bacteria or 2x10^7 fungi per column, eluting in 25-100 ul - the standard choice for typical 1-5 ml bacterial cultures. The Microprep handles smaller inputs (up to 1x10^7 bacteria) and elutes in as little as 6 ul, concentrating RNA for sensitive applications such as single-cell-equivalent samples or very low-input transcriptomics.</p>
<p><strong>Bead Size for Target Organism</strong></p>
<p>Pre-mixed 0.1 mm + 0.5 mm BashingBead tubes in Zymo kits handle bacteria (0.1 mm) and fungi (0.5 mm) without selecting bead size. For pure Gram-negative bacterial cultures that do not require bead beating, direct lysis in buffer is available as a faster protocol option that skips the bead-beating step entirely.</p>
<p><strong>On-Column DNase I Treatment</strong></p>
<p>Include DNase I for all RT-qPCR and RNA-seq applications. Bacterial gDNA is abundant and co-purifies efficiently under silica-membrane binding conditions; without DNase treatment, gDNA contamination produces false amplification in RT-qPCR assays targeting genes with no introns. DNase I Reaction Mix is included in Zymo Quick-RNA Fungal/Bacterial kits.</p>
<p><strong>Elution Volume</strong></p>
<p>Elute in 25-50 ul for standard RT-qPCR (2-5 ul per reaction provides 5-25 RNA equivalents per assay). For RNA-seq library preparation requiring &gt;=200 ng input, use 50-100 ul elution volume to recover more total RNA mass; then measure concentration by Qubit and adjust for library prep.</p>
<p><strong>Storage of Extracted RNA</strong></p>
<p>Store spin-column bacterial RNA at -80C in single-use aliquots; mRNA from bacteria is AU-rich and degrades rapidly through multiple freeze-thaw cycles. Use within 6 months for transcriptomic applications; RT-qPCR targets with high expression levels remain quantifiable for 12+ months at -80C.</p>
<p>&nbsp;</p>
<h2>Specifications Context</h2>
<p>&nbsp;</p>
<p>Target quality metrics for spin-column bacterial/fungal RNA: A260/A280 &gt;= 1.8, A260/A230 &gt;= 1.5, and RIN &gt;= 7 from log-phase cultures processed within 60 seconds of harvest. Zymo Research Quick-RNA Fungal/Bacterial kits use the same Zymo-Spin column technology found across the Quick-RNA product line and are validated for inhibitor-free RNA suitable for NGS, RT-PCR, microarray, and hybridization. The Quick-RNA Fungal/Bacterial Miniprep is one of the most widely used spin-column formats for bacterial and fungal RNA in academic molecular microbiology research, with validated protocols for S. cerevisiae, C. albicans, A. fumigatus, and standard Gram-positive and Gram-negative species.&nbsp;</p>
<section class="mbp-contact-cta"></section>
<p dir="ltr" role="presentation">&nbsp;</p>
<p dir="ltr"><strong><a href="https://mbpinc.net/contact-us">Contact the MBP team</a></strong> for kit selection guidance, bulk pricing, and Zymo Research protocol adaptation for non-standard organisms.</p>

Spin-column

Bacterial/Fungal RNA/ Spin-column

MSDS

Protocol

Quick-RNA™ Fungal/Bacterial MicroPrep Kit (50 Preps.)

<p>Quick-RNA™ Fungal/Bacterial MicroPrep Kit R2010 by Zymo Research — 50 preps, RNA ≥17 nt, 10 µg binding, ≥6 µL elution, ≤10 min. ZR BashingBead Lysis Tubes for mechanical disruption of fungal (Candida, Aspergillus, Saccharomyces) and bacterial cell walls. A260/A280 &gt;1.8. Order via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.</p>

Applicable For

Elution Volume

Equipment

Processing Time

Processing Volume

Purity

Sample Source

Size Range

Type

Yield

Manufacturer

Shipping Condition

Storage Condition

<h2>What Are Spin-Column Total RNA Kits?</h2>
<p>&nbsp;</p>
<p>Spin-column total RNA kits are the most widely used format for manual RNA purification in molecular biology laboratories. The technology relies on silica membrane chemistry: under chaotropic (guanidinium-based) lysis conditions, RNA adsorbs selectively to the silica membrane of the column; sequential ethanol-based wash steps remove proteins, salts, and gDNA fragments; and a low-ionic-strength buffer or RNase-free water then releases the purified RNA. Standard mini-format kits accept cell or tissue lysate from inputs of 1x10^4-1x10^7 cells or 5-100 mg tissue, bind up to 100 ug RNA, and complete the protocol in 15-30 minutes. The eluted RNA is suitable for RT-PCR, qRT-PCR, RNA sequencing, Northern blot, and hybridization without additional cleanup<strong>.&nbsp;</strong>Spin-column RNA kits are the default choice for 1-24 samples per run in any lab that does not require automated magnetic-bead processing.</p>
<p>&nbsp;</p>
<h2 dir="ltr">What you will find:</h2>
<p dir="ltr">&nbsp;</p>
<ul>
<li dir="ltr" aria-level="1">
<p dir="ltr" role="presentation">Quick-RNA Microprep and Miniprep Kits for low-volume elution and highly concentrated recovery, providing ultra-clean and phenol-free transcript isolation</p>
</li>
<li dir="ltr" aria-level="1">
<p dir="ltr" role="presentation">Direct-zol&trade; RNA Kits to bypass tedious phase separation steps, allowing researchers to pump TRIzol-lysed samples directly into spin-columns&nbsp;</p>
</li>
<li dir="ltr" aria-level="1">
<p dir="ltr" role="presentation">Direct-zol&trade; DNA/RNA Parallel Modules are engineered for the non-destructive, simultaneous isolation of DNA and RNA fractions.</p>
</li>
<li dir="ltr" aria-level="1">
<p dir="ltr" role="presentation">ZymoBIOMICS&trade; RNA Mini Kits for uniform microbial profiling featuring host-zeroing lysis mechanics</p>
</li>
<li dir="ltr" aria-level="1">
<p dir="ltr" role="presentation">Quick-RNA&trade; Midiprep Assemblies to capture larger quantities of high-purity total transcripts efficiently, tailored for bulk processing&nbsp;</p>
</li>
</ul>
<p dir="ltr">&nbsp;</p>
<h2>How to Choose Spin-Column Total RNA Kits</h2>
<p>&nbsp;</p>
<p><strong>Column Binding Capacity and Elution Volume</strong></p>
<p>Mini-format columns bind up to 100 ug total RNA and elute in 10-100 uL. For samples with very low RNA content (needle biopsies, laser-capture microdissection sections, small cell numbers &lt;=10^4), choose micro-format columns with 8-12 uL minimum elution volume to maximize concentration. Eluting in &lt;30 uL with high RNA concentration increases sensitivity in downstream RT-qPCR.</p>
<p><strong>Small RNA Retention</strong></p>
<p>This is the most important differentiator between kit brands. Kits using Zymo-Spin column technology with proprietary binding buffers retain miRNA and small RNA species &gt;=17 nt in the total RNA fraction. Competing kits using standard high-ethanol wash chemistry typically lose RNA &lt;200 nt in the wash steps. If your workflow includes small RNA profiling, miRNA RT-qPCR, or small RNA-seq, verify that the kit retains &gt;=17 nt RNA before purchasing.</p>
<p><strong>On-Column DNase Treatment</strong></p>
<p>Included RNase-free DNase I that degrades gDNA on the column during the wash stage eliminates the need for post-elution DNase treatment. This matters for RT-qPCR with primers spanning a small intron, or for RNA-seq where even trace gDNA contamination generates false mapping reads. DNase-treated RNA typically passes a no-RT PCR control at Ct &gt;35.</p>
<p><strong>Sample Type: Cells vs Tissue</strong></p>
<p>Cell lysis buffer volumes and homogenization requirements differ by sample type. Cultured cells lyse directly in lysis buffer with vortexing; tissue samples require homogenization to disrupt cell structure before contact with the lysis buffer. For fibrous tissues (muscle, heart), use a bead-mill homogenizer or rotor-stator; for soft tissues (liver, brain), a disposable pestle or needle shearing is sufficient. Some kits include TissueLyser-compatible bead tubes or QIAshredder-equivalent homogenizer columns.</p>
<p>&nbsp;</p>
<h2>Specifications Context</h2>
<p>&nbsp;</p>
<p>Spin-column total RNA purity targets: A260/A280 ratio 1.8-2.1; A260/A230 ratio &gt;1.8. RNA integrity number (RIN) from fresh cultured cells or snap-frozen tissue is routinely 8-10 with validated kits. Zymo Research Quick-RNA Mini and Miniprep kits are among the most cited spin-column RNA isolation kits in published RT-PCR and RNA-seq methods; ABM RNA extraction kits offer validated protocols for bacteria, yeast, and fungi not covered by many standard cell/tissue kits. All kits supplied by MBP are Research Use Only (RUO).</p>
<section class="mbp-contact-cta">
<p>&nbsp;</p>
<p>MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<strong><a href="https://mbpinc.net/contact-us">Contact the MBP team</a></strong> for personalized guidance.</p>
</section>

DataSheet

Quick-RNA™ MidiPrep (25 Preps.)

<p>Quick-RNA MidiPrep Kit (R1056) by Zymo Research — up to 1 mg RNA yield per prep, ≥17 nt, A260/A280 and A260/A230 &gt;1.8, ≥200 µl elution. 25 Preps, room temperature storage. Optimized for cells and soft tissue.</p>

<h2>What are Spin-Column Insect RNA Extraction Kits?</h2>
<p>&nbsp;</p>
<p>Spin-column insect RNA extraction kits purify total RNA from whole arthropod specimens and insect tissue using ultra-high-density BashingBead mechanical homogenization in a chaotropic lysis buffer, followed by a two-column silica-membrane purification workflow in a standard microcentrifuge. The Zymo Quick-RNA Tissue/Insect Microprep Kit (R2030) uses a Zymo-Spin IIIC column to capture and discard genomic DNA in the flow-through, then a Zymo-Spin IC column to bind, wash, and elute total RNA, including small RNAs &gt;=17 nt. The full protocol completes in approximately 10 minutes for batches of 1-12 specimens. Choose the spin-column insect RNA format for manual extraction of single insects to small batches, for field work using DNA/RNA Shield stabilization, and for studies requiring co-isolation of miRNA and siRNA from the same arthropod specimen.</p>
<p dir="ltr">&nbsp;</p>
<h2 dir="ltr"><span style="font-family: arial, helvetica, sans-serif;">What you will find:</span></h2>
<p dir="ltr" role="presentation">&nbsp;</p>
<ul>
<li data-section-id="bn9qob" data-start="0" data-end="116">Quick-RNA&trade; Tissue/Insect MicroPrep Kit for rapid isolation of ultra-pure total RNA from tissue and insect samples.</li>
<li data-section-id="1d6veh7" data-start="233" data-end="340">Ultra-clean spin-column technology designed to maximize RNA purity and recovery from small sample inputs.</li>
<li data-section-id="o7nhuv" data-start="341" data-end="463">RNA extraction solutions optimized for challenging biological specimens, including insect tissues and dissected samples.</li>
<li data-section-id="18gssqf" data-start="464" data-end="573">High-quality total RNA preparation suitable for RT-PCR, qPCR, RNA sequencing, and gene expression analysis.</li>
<li data-section-id="lpdewh" data-start="574" data-end="671" data-is-last-node="">Streamlined purification protocols that reduce processing time while maintaining RNA integrity</li>
</ul>
<p>&nbsp;</p>
<h2>How to Choose a Spin-Column Insect RNA Kit</h2>
<p>&nbsp;</p>
<p><strong>Microprep vs. Miniprep Format</strong></p>
<p>The Microprep kit (Zymo R2030) processes specimens up to 10 mg and elutes in 6-25 ul, concentrating RNA for sensitive RT-qPCR from single small insects. The Miniprep format handles up to 30 mg of tissue - appropriate for dissected insect organs, larger arthropods, or small pools of 5-10 insects - and elutes in 25-100 ul for applications requiring higher total RNA mass.</p>
<p><strong>Two-Column DNA Depletion vs. On-Column DNase</strong></p>
<p>The two-column design (IIIC + IC column) in Zymo insect kits physically separates gDNA by binding capacity difference, eliminating a separate DNase incubation step. This is faster but less stringent than on-column DNase I treatment. For RNA-seq applications where any gDNA carryover introduces mapping artefacts, add an optional DNase I step; for RT-qPCR with intron-spanning primers, the two-column depletion alone is generally sufficient.</p>
<p><strong>Bead Beating Instrument</strong></p>
<p>A benchtop vortex (Vortex-Genie 2) with a horizontal microtube adapter provides validated lysis for the Zymo BashingBead Lysis Tubes at maximum speed for 5 minutes. A dedicated bead mill (e.g., Precellys, MP FastPrep) achieves more consistent disruption for hard-bodied arthropods such as hard ticks and beetles and is preferred when processing more than 24 specimens per session.</p>
<p><strong>Specimen Stabilization Compatibility</strong></p>
<p>Specimens in Zymo DNA/RNA Shield load directly into lysis tubes without additional washes or centrifugation steps. If specimens were field-collected in dry ice or liquid nitrogen, thaw at room temperature in lysis buffer and add to BashingBead tubes immediately - do not allow thawed specimens to sit at room temperature without lysis buffer, as RNase activity resumes rapidly on thawing.</p>
<p><strong>Vector Surveillance Pooling</strong></p>
<p>For arbovirus surveillance, pool up to 50 mosquitoes in the same lysis tube, cap tightly, and bead-beat at maximum speed for 10 minutes. Transfer 200-400 ul of clarified lysate (post-centrifugation) per column; multiple column reloads can process the remaining volume. Validate pool sensitivity with a spiked positive control before deploying in routine surveillance.</p>
<p>&nbsp;</p>
<h2>Specifications Context</h2>
<p>&nbsp;</p>
<p>Performance benchmarks for spin-column insect RNA: A260/A280 &gt;= 1.8, A260/A230 &gt;= 1.8, and RNA yield up to 10 ug from a single Aedes aegypti adult female. The Zymo Quick-RNA Tissue/Insect Microprep Kit is validated for RT-PCR, hybridization, microarray, and Next-Gen sequencing applications, and its two-column architecture is designed to deliver RNA free of PCR inhibitors - a critical requirement for arbovirus RT-qPCR assays where false-negative results from inhibitor carryover have direct public health implications. This kit is the primary insect RNA extraction format used in Drosophila immunity and mosquito vector competence research, with published protocols referencing BashingBead lysis in studies from institutions including Howard Hughes Medical Institute and Vanderbilt University.</p>
<section class="mbp-contact-cta">
<h2>&nbsp;</h2>
<p>MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<strong><a href="https://mbpinc.net/contact-us">Contact the MBP team</a></strong> for personalized guidance.</p>
</section>

Spin-Column

Quick-RNA™ Tissue/Insect MicroPrep Kit (50 Preps.)

<p>Quick-RNA™ Tissue/Insect MicroPrep Kit R2030 by Zymo Research is a 50-prep kit for total RNA isolation (≥17 nt, including miRNAs) from insects, arthropods (mosquitoes, ticks, Drosophila), and tough-to-lyse tissue in 1–20 mg amounts. Includes BashingBead lysis tubes, Zymo-Spin IC and IIICG columns, minimum elution 6 µl, binding capacity 10 µg, purity A260/A280 >1.8. All components stored at room temperature.</p>

<h2>What Are Zymo Research Buffers?</h2>
<p>Zymo Research buffers are formulation-specific reagents used at defined steps &mdash; lysis, binding, wash, and elution &mdash; in Zymo-Spin column-based purification protocols for DNA, RNA, and bisulfite-converted DNA. Each buffer is optimized for a specific kit family: DNA Wash Buffer concentrate (requires ethanol addition before use) removes salts and contaminants before elution from Zymo-Spin silica columns; DNA Elution Buffer (10 mM Tris, pH 8.5, 0.1 mM EDTA) releases purified DNA from the matrix; RNA Wash Buffer supports RNA extraction kits including Quick-RNA and Direct-zol lines; M-Elution Buffer is designed for eluting bisulfite-converted DNA in EZ DNA Methylation kits. Researchers typically order these separately when a single buffer component runs out before the rest of the kit, or when scaling up a protocol beyond the volume included.</p>
<p><strong>TL;DR:</strong> Order Zymo buffers individually to replenish a specific step in your existing Zymo workflow without replacing the full kit.</p>
<h2>How to Choose Zymo Research Buffers</h2>
<h3>Match the buffer to your kit family</h3>
<p>Every Zymo buffer is paired to a specific kit line. DNA Wash Buffer and DNA/RNA Wash Buffer concentrate are used in DNA Clean &amp; Concentrator, Quick-DNA, and Zymoclean Gel DNA Recovery kits. RNA Wash Buffer pairs with Quick-RNA kits. ZymoBIOMICS DNA Wash Buffer 1 and Wash Buffer 2 are specific to the ZymoBIOMICS microbiomics line. Substituting a buffer from a different kit family can compromise yield or purity.</p>
<h3>Confirm ethanol addition requirements before use</h3>
<p>Several Zymo wash buffer concentrates require addition of 96-100% ethanol to the bottle before first use &mdash; the label states the exact volume. DNA Wash Buffer concentrate, DNA/RNA Wash Buffer concentrate, and Urine DNA Wash Buffer concentrate all require this step. Pre-made (non-concentrate) versions such as RNA Wash Buffer are supplied ready to use.</p>
<h3>Select the right volume for your throughput</h3>
<p>Zymo buffers are available in sizes from 6 ml (single-experiment replenishment) up to 500 ml (Buffer P1 Red, for high-volume plasmid prep labs). For labs processing more than 50 samples per week, ordering the largest available size reduces per-sample cost and the frequency of reordering.</p>
<h3>Choose elution buffer chemistry based on downstream application</h3>
<p>DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA) is the standard for most PCR and sequencing applications. For applications sensitive to EDTA &mdash; such as some restriction enzyme digests or direct use with divalent cation-dependent enzymes &mdash; eluting in molecular-grade water or a Tris-only buffer is preferable. M-Elution Buffer is specifically formulated for bisulfite-converted DNA and should not be substituted with standard elution buffer in methylation workflows.</p>
<h3>Verify research-use-only status</h3>
<p>All Zymo Research buffers sold through MBP are for research use only (RUO). They are not cleared for in vitro diagnostic use. For clinical or diagnostic laboratory applications, confirm regulatory status with your institutional compliance officer before use.</p>
<h2>Compare Zymo Buffer Types</h2>
<table>
<thead>
<tr>
<th>Buffer Type</th>
<th>Primary Application</th>
<th>Kit Family</th>
<th>Format Available</th>
</tr>
</thead>
<tbody>
<tr>
<td>DNA Wash Buffer (concentrate)</td>
<td>Salt removal before DNA elution</td>
<td>DNA Clean &amp; Concentrator, Quick-DNA</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA/RNA Wash Buffer (concentrate)</td>
<td>Salt removal for dual DNA/RNA preps</td>
<td>Quick-DNA/RNA kits</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA)</td>
<td>DNA release from silica column</td>
<td>All Zymo DNA purification</td>
<td>16 ml &ndash; available sizes vary</td>
</tr>
<tr>
<td>RNA Wash Buffer</td>
<td>RNA purification wash step</td>
<td>Quick-RNA kits</td>
<td>Ready-to-use</td>
</tr>
<tr>
<td>M-Elution Buffer</td>
<td>Elution of bisulfite-converted DNA</td>
<td>EZ DNA Methylation kit families</td>
<td>Small volume</td>
</tr>
<tr>
<td>ZymoBIOMICS DNA Wash Buffer 2</td>
<td>Metagenomic/microbiomic DNA cleanup</td>
<td>ZymoBIOMICS DNA kits</td>
<td>Sold separately</td>
</tr>
<tr>
<td>Buffer P1 Red</td>
<td>Alkaline lysis resuspension (plasmid prep)</td>
<td>Zyppy Plasmid Miniprep</td>
<td>10 ml &ndash; 500 ml</td>
</tr>
</tbody>
</table>
<h2>Specifications Context</h2>
<p>Zymo wash buffer concentrates are supplied at a defined fold-concentration (commonly 4x or 6x) and require ethanol addition to reach working strength &mdash; the exact ratio is printed on the bottle and in the kit protocol. Elution buffer volumes for Zymo-Spin columns typically range from 6 to 100 &micro;l per elution; larger elution volumes improve total yield at the cost of DNA concentration. For DNA elution from Zymo-Spin columns, the protocol recommends a minimum 1-minute contact time between elution buffer and the silica matrix to maximize recovery of fragments 10 kb and above. As of 2025, Zymo Research tests all buffer lots for performance consistency, with integrity guaranteed for up to one year from purchase date.</p>
<p>Browse the full <a href="https://www.notion.so/reagents">Zymo Research reagents collection at MBP</a> for kits and columns that use these buffers, or visit our <a href="https://www.notion.so/reagents/buffers">reagent buffers category</a> for non-Zymo buffer options including ABM and other brands. For volume orders or purchase orders, use the <a href="https://www.notion.so/quickorder">Quick Order</a> tool or <a href="https://www.notion.so/contact-us">contact us</a> directly &mdash; MBP is a registered vendor for institutional procurement systems.</p>
<p><em>Last updated: June 2025</em></p>
<p>&nbsp;</p>

Zymo Buffers

Quick-cfDNA/cfRNA™ Binding Buffer (150 ml)

<p>Quick-cfDNA/cfRNA™ Binding Buffer R1072-2-150 by Zymo Research is a 150 ml standalone replacement component for the Quick-cfDNA/cfRNA™ Serum and Plasma Kit (R1072). Added 1:1 to proteinase K-digested serum or plasma, it conditions the sample for efficient cfDNA and cfRNA binding to the purification column. Stored at room temperature.</p>

Quick-cfDNA/cfRNA™ Digestion Buffer (150 ml)

<p>Quick-cfDNA/cfRNA™ Digestion Buffer (R1072-1-150) by Zymo Research is a 150 ml proteinase K digestion buffer used at a 1:1 ratio with serum or plasma in the Quick-cfDNA/cfRNA Serum & Plasma Kit (R1072). Applied at 37°C for 2 hours, it prepares liquid biopsy samples for cfDNA and cfRNA column-based purification.</p>

<h2>What are Cell-Free DNA/RNA Co-Extraction Kits?</h2>
<p>&nbsp;</p>
<p>Cell-free DNA/RNA co-extraction kits simultaneously isolate circulating cell-free DNA (cfDNA -- ctDNA fragments of 130-250 bp, apoptotic DNA) and cell-free RNA (cfRNA -- microRNAs, lncRNAs, exosomal RNA, circulating mRNA fragments) from the same plasma, serum, or urine sample without phenol-chloroform or protein precipitation, delivering both analytes for parallel liquid biopsy genomic and transcriptomic analysis from a single 1-4 ml blood draw. Unlike dedicated cfDNA kits that miss circulating RNA species, or cfRNA kits that under-recover short cfDNA fragments, co-extraction kits are formulated to bind both nucleic acid classes - including protein-bound RNA, exosomal RNA, and miRNA - from cell-free matrices where total nucleic acid concentration is 1-100 ng/ml. Use a cfDNA/cfRNA co-extraction kit when your liquid biopsy workflow requires both somatic mutation analysis (ctDNA sequencing) and RNA expression profiling (circulating miRNA or cfRNA-seq) from the same blood draw, or when sample volume is limited and splitting between separate DNA and RNA kits would leave insufficient input for either.</p>
<p>&nbsp;</p>
<h2 dir="ltr">What you will find:</h2>
<p dir="ltr">&nbsp;</p>
<ul>
<li dir="ltr" aria-level="1">
<p dir="ltr" role="presentation">Quick-cfDNA/cfRNA&trade; Serum &amp; Plasma Kit: An efficient spin-column setup tailored for sample volumes up to 3 ml, providing reduced sequence bias and recovering as much as 515 times more microRNA.</p>
</li>
<li dir="ltr" aria-level="1">
<p dir="ltr" role="presentation">High-Purity Elution Technology: Nuclease-free methods enabling the separate or co-elution of nucleic acids, ensuring transfection-grade purity for delicate bioinformatics and RT-qPCR uses.</p>
</li>
</ul>
<p><strong>&nbsp;</strong></p>
<h2>How to Choose a cfDNA/cfRNA Co-Extraction Kit</h2>
<p>&nbsp;</p>
<p><strong>Input Volume and Assay Sensitivity</strong></p>
<p>cfDNA concentration in cancer patient plasma ranges from 1-1,000 ng/ml depending on tumor stage and type; circulating miRNA concentration is typically 1-100 pg/ml. For low-MAF ctDNA detection (&lt;0.1%), 2-4 ml plasma input is required to obtain sufficient copies for ddPCR or ultra-deep sequencing. For cfRNA profiling only, 200 &mu;L-1 mL is sufficient for miRNA RT-qPCR panels. The Zymo Quick-cfDNA/cfRNA Kit scales linearly up to 3 ml input.</p>
<p><strong>Co-Elution vs. Separate Fractions</strong></p>
<p>The Zymo Quick-cfDNA/cfRNA Serum &amp; Plasma Kit offers the option to co-elute DNA and RNA into a single fraction or to collect separate fractions. Co-elution maximizes nucleic acid recovery in the smallest volume -- optimal for low-abundance targets; separate fractions are needed when DNA-specific (bisulfite sequencing, whole-exome) and RNA-specific (rRNA depletion, miRNA-seq library) downstream protocols cannot use the same input.</p>
<p><strong>miRNA Recovery</strong></p>
<p>Circulating miRNAs (18-22 nt) are small, low-abundance, and easily lost during standard DNA extraction. Confirm the co-extraction kit explicitly recovers small RNAs &lt;200 nt from plasma. The Zymo Quick-cfDNA/cfRNA Kit recovers up to 515x more microRNA than conventional phase-separation methods -- a validated difference confirmed in the Zymo product datasheet.</p>
<p><strong>Phenol-Free Chemistry</strong></p>
<p>For high-throughput liquid biopsy programs, phenol-free kits eliminate fume-hood requirements and toxic waste handling. The QIAGEN ccfDNA/RNA Kit and Zymo Quick-cfDNA/cfRNA Kit are both phenol-free; automated protocols using these kits can process large cohort batches without hazardous chemical handling steps.</p>
<p><strong>Pre-Analytical Standardization</strong></p>
<p>cfDNA/cfRNA concentrations vary with blood collection tube type, time-to-processing, and centrifugation protocol. Standardize on EDTA tubes + two-spin plasma preparation (300xg then 16,000xg) and process within 6 hours of collection or store at -80 degrees C. For samples requiring extended transport, Streck Cell-Free DNA BCT or cfRNA preservation tubes stabilize both analytes at room temperature for up to 7 days before extraction.</p>
<p>&nbsp;</p>
<h2>Specifications Context</h2>
<p>&nbsp;</p>
<p>The Zymo Quick-cfDNA/cfRNA Serum &amp; Plasma Kit enables simple spin-column isolation of all circulating cell-free DNA and RNA from serum, plasma, and biological fluids, recovering protein-bound, exosomal, miRNA, and other small RNA in addition to cfDNA. Cell-free nucleic acids scale linearly with input volume up to 3 ml. A 2022 digital PCR comparative study (PMC9805675) evaluated multiple cfDNA/cfRNA co-purification kits across plasma input volumes of 0.06-4 ml, finding that combined cfDNA/cfRNA quantification produces higher total nucleic acid concentrations than parallel separate quantification. cfDNA/cfRNA multi-omic liquid biopsy is an active area of clinical translational research for non-invasive cancer diagnosis and treatment monitoring, with studies underway at MD Anderson Cancer Center and Vanderbilt University using co-extraction formats to maximize information per blood draw.</p>
<p>&nbsp;</p>
<p dir="ltr">Close the gap between liquid biopsy and groundbreaking discovery&mdash;<strong><a href="https://mbpinc.net/contact-us">reach out to MBP Inc.</a> </strong>now to obtain a quote for your next-generation sequencing requirements.</p>

Cell-free DNA/RNA

Quick-cfDNA/cfRNA™ Serum & Plasma Kit (50 Preps.)

<p>Quick-cfDNA/cfRNA™ Serum &amp; Plasma Kit (R1072) by Zymo Research — 50 preps. Isolates cfDNA (≥50 bp) and cfRNA/miRNA (≥17 nt) from up to 3 ml serum, plasma, CSF, urine, or biological fluids. Elution 6–15 µl. Up to 515× more microRNA recovery. Co-purification (~30 min) or parallel purification modes.</p>

Required Equipment

Sample Types

Size

<h2>What Are Cell-Free DNA Extraction Kits?</h2>
<p>&nbsp;</p>
<p>A cell-free DNA (cfDNA) extraction kit purifies circulating cfDNA fragments from plasma, serum, or urine using silica spin-column or magnetic bead-based technologies. These kits are optimized to recover the short DNA fragments commonly found in liquid biopsy samples for downstream applications such as PCR, qPCR, next-generation sequencing (NGS), and methylation analysis. Choose a cfDNA-specific kit over a general genomic DNA kit whenever short circulating DNA fragments are the primary target.</p>
<p>&nbsp;</p>
<h2>What you will find:</h2>
<p>&nbsp;</p>
<ul>
<li>MagBio cfKapture ccfDNA Extraction Kit: for cell-free DNA isolation using advanced magnetic bead-based technology.</li>
<li>MAGicBead&trade; cfDNA Isolation Kit: for automated, high-throughput liquid biopsy workflows using high-binding paramagnetic particles.</li>
<li>Quick-cfDNA&trade; Serum &amp; Plasma Kit: for streamlined manual benchtop purification using rapid spin-column technology.</li>
</ul>
<p>&nbsp;</p>
<h2>How to Choose a Cell-Free DNA Extraction Kit</h2>
<p>&nbsp;</p>
<p><strong>Input Volume Range</strong></p>
<p>Choose a kit compatible with your typical plasma, serum, or urine input volume. Larger sample volumes generally increase total cfDNA recovery but may require additional reagents and longer processing times.</p>
<p><strong>Column-Based vs. Magnetic Bead-Based Chemistry</strong></p>
<p>Silica spin-column kits are widely used for manual purification workflows, while magnetic bead-based kits simplify automation and high-throughput processing on compatible liquid handling platforms.</p>
<p><strong>Elution Volume for Downstream Assays</strong></p>
<p>Select an elution volume that matches the input requirements of your downstream applications, such as PCR, qPCR, or NGS library preparation, to minimize additional concentration or dilution steps.</p>
<p><strong>Combined DNA/RNA Recovery</strong></p>
<p>If your workflow requires analysis of both circulating DNA and RNA from the same specimen, consider kits designed to recover multiple nucleic acid types from a single sample.</p>
<p>&nbsp;</p>
<h2>Specifications Context</h2>
<p>&nbsp;</p>
<p>Key considerations when selecting a cell-free DNA extraction kit include sample input volume, purification chemistry, compatibility with manual or automated workflows, and elution volume for downstream molecular assays. Magnetic bead-based systems are commonly selected for scalable, automated liquid biopsy workflows, while spin-column kits provide reliable manual purification for routine laboratory use. The optimal kit depends on your sample type, throughput requirements, and downstream analytical methods.</p>
<section class="mbp-contact-cta">
<p>&nbsp;</p>
<p>MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<strong><a href="https://mbpinc.net/contact-us">Contact the MBP team</a> </strong>for personalized guidance.</p>
</section>

Cell-Free DNA

D4076-Data Sheet

D4076 - MSDS

D4076-1-32 - MSDS

D4076-2-170 - MSDS

D4076-3-10 - MSDS

D4076-4-12 - MSDS

D4076-A - MSDS

D4076-Protocol

Quick-cfDNA™ Serum & Plasma Kit

<p>Quick-cfDNA™ Serum &amp; Plasma Kit (D4076) by Zymo Research — 50 preps, purifies cfDNA ≥100 bp from up to 10 ml serum/plasma. Also compatible with amniotic fluid, CSF, and saliva. Elution ≥35 µl. Compatible with Streck and PAXgene tubes.</p>

DNA Purity

DNA Recovery

DNA Storage

Processing volume

Recovery Volume

Sample Sources

Quick-cfDNA™ Serum & Plasma Kit (50 Preps.)

S&P 5X Digestion Buffer (32 ml)

S&P DNA Binding Buffer (170 ml)

S&P DNA Prep Buffer (10 ml)

S&P DNA Wash Buffer (Concentrate) (12 ml)

Quick-cfDNA™ Serum & Plasma Buffer Set

Quick-cfRNA™ Binding Buffer (100 ml)

<p>R1059-4-100 by Zymo Research is the Quick-cfRNA&trade; Binding Buffer supplied in a 100 ml volume for cell-free RNA (cfRNA) isolation from serum, plasma, cerebrospinal fluid, and urine. Added at a 1:1 ratio to the proteinase K-digested sample, this buffer promotes RNA adsorption onto Zymo-Spin IC Columns and enables recovery of cfRNA fragments &ge;17 nucleotides in length, including miRNA and exosomal RNA.</p>
<p>Storage is at room temperature. The buffer is a standalone refill component for the Quick-cfRNA Serum &amp; Plasma Kit (R1059) and is not interchangeable with the Quick-cfDNA/cfRNA Binding Buffer (R1072-2-150), which serves a different dual-extraction kit.</p>
<p>MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, Eurofins, and many other institutes across the USA. Place your order via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or contact <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.</p>

Quick-cfRNA™ Digestion Buffer (50 ml)

<p>Quick-cfRNA™ Digestion Buffer R1059-3-50 by Zymo Research — 50 ml, room-temperature stable — digests proteins prior to cell-free RNA isolation from serum and plasma. Use 200 µL per 200 µL sample with Proteinase K (37°C, 2 hours); Streck Blood Tube samples require an additional 55°C pre-digestion step. Available from MBP via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.</p>

<h2>What are Cell-Free RNA Extraction Kits?</h2>
<p>&nbsp;</p>
<p>Cell-free RNA (cfRNA) extraction kits isolate circulating RNA molecules - microRNAs, long non-coding RNAs, exosomal RNA, and mRNA fragments - from blood plasma, serum, urine, cerebrospinal fluid, and saliva, where endogenous RNA concentrations range from 1-100 ng/ml. Unlike standard total RNA kits designed for cell lysates containing micrograms of intracellular RNA per sample, cell-free RNA kits are built for the low-abundance, fragmented, and often vesicle-packaged RNA species present in acellular body fluids. They incorporate carrier RNA to prevent adsorption loss, handle input volumes of 200 ul to 5 mL, and use binding chemistries optimized for small RNAs &lt;200 nt. Use a cell-free RNA kit - not a standard total RNA or viral RNA kit - whenever your input is plasma, serum, urine, or another cell-depleted biofluid and your target is circulating miRNA, cfRNA, or exosomal RNA.</p>
<p dir="ltr">&nbsp;</p>
<h2 dir="ltr"><span style="font-family: arial, helvetica, sans-serif;">What you will find:</span></h2>
<p>&nbsp;</p>
<ul>
<li data-section-id="1fsegxt" data-start="25" data-end="105">Rapid purification of circulating cell-free RNA (cfRNA) from plasma and serum.</li>
<li data-section-id="dahqiz" data-start="106" data-end="181">Efficient recovery of low-abundance cell-free RNA from biological fluids.</li>
<li data-section-id="85g4hb" data-start="182" data-end="245">Zymo-Spin&trade; spin-column technology for reliable RNA isolation.</li>
<li data-section-id="exy4np" data-start="246" data-end="296">Streamlined workflow for fast sample processing.</li>
<li data-section-id="mtq4uw" data-start="297" data-end="369" data-is-last-node="">Purified cfRNA suitable for downstream molecular biology applications.</li>
</ul>
<p>&nbsp;</p>
<h2>How to Choose a Cell-Free RNA Extraction Kit</h2>
<p>&nbsp;</p>
<p><strong>Input Volume and Throughput</strong></p>
<p>Standard cell-free RNA kits accept 200 &mu;L-1 mL of plasma or serum per extraction; this input volume is appropriate for RT-qPCR quantification of abundant miRNA species and exosomal RNA panels. For discovery-phase cfRNA-seq, where rare transcripts are targets, use high-volume extraction formats to increase RNA mass from low-abundance circulating species.</p>
<p><strong>Small RNA Enrichment</strong></p>
<p>Circulating miRNAs are the most studied cfRNA analytes; kits that enrich for RNA &lt;200 nt deliver higher sensitivity for miRNA panels than kits recovering large and small RNAs together.&nbsp;</p>
<p><strong>Carrier RNA and Spike-In Controls</strong></p>
<p>Every cell-free RNA extraction should include both a carrier RNA (added to the lysis buffer to improve recovery) and an exogenous spike-in control RNA - typically Caenorhabditis elegans miR-39 (cel-miR-39) at 1.6 &times; 10^8 copies per extraction - added at the lysis step. Spike-ins normalize inter-sample variation in extraction efficiency and enable detection of failed extractions. Confirm whether carrier RNA is supplied in the kit or purchased separately.</p>
<p><strong>Magnetic Bead vs. Spin-Column Format</strong></p>
<p>Spin-column cfRNA kits (Zymo Quick-RNA Serum &amp; Plasma) are the standard for 1-24 samples per session and require only a microcentrifuge. Magnetic bead formats are preferred for higher-volume inputs and automation, and demonstrate superior recovery of fragmented low-abundance RNA from dilute matrices in published comparative studies.</p>
<p><strong>Phenol-Free vs. Phenol-Containing Lysis</strong></p>
<p>Most current cell-free RNA kits use guanidinium-based, phenol-free lysis buffers, avoiding the hazardous handling requirements of phenol-containing reagents. Phenol-chloroform-based extraction of cfRNA is used in research settings for the highest yield but introduces extraction bias for certain miRNA species and requires additional purification steps; column-based phenol-free kits are preferred for clinical and high-throughput biomarker applications.</p>
<p>&nbsp;</p>
<h2>Specifications Context</h2>
<p>&nbsp;</p>
<p>Performance benchmarks for cell-free RNA extraction: recovery of cel-miR-39 spike-in within 2 Ct of the expected value, A260/A280 &gt;= 1.7 (lower values are acceptable for extremely dilute cfRNA samples), and consistent inter-sample Ct variation &lt;1.5 Ct for reference miRNAs across a batch. Circulating miRNA biomarkers -- including miR-21, miR-155, miR-210, miR-126, and miR-200 family members - are under clinical evaluation as non-invasive cancer, cardiovascular, and neurological disease biomarkers, with studies published by groups at institutions including MD Anderson Cancer Center and Vanderbilt University. Pre-analytical variables -- plasma preparation method, freeze-thaw cycles, hemolysis - are major sources of inter-study variability in cfRNA data; standardized two-spin plasma preparation protocols are recommended before extraction.</p>
<p>&nbsp;</p>
<p>For ordering assistance, bulk pricing, or protocol guidance on selecting the right kit for your sample type, <a href="https://mbpinc.net/contact-us"><strong>contact the MBP team</strong></a>.&nbsp;</p>

Cell-free RNA

White Paper

Quick-cfRNA™ Serum and Plasma (50 Preps.)

<p>Quick-cfRNA™ Serum and Plasma Kit (R1059) by Zymo Research isolates cell-free RNA (≥17 nt, including microRNA) from up to 3 mL of plasma, serum, CSF, amniotic fluid, or urine in a spin-column workflow with 6–15 µl concentrated elution. No phenol-chloroform required. 50-prep format for liquid biopsy and cfRNA biomarker research.</p>

Sample Type

RAD Buffer (RNA Agarose Dissolving Buffer) (50 ml)

<p>R1011-1-50 by Zymo Research is a 50 mL RAD Buffer (RNA Agarose Dissolving Buffer) that simultaneously dissolves agarose gel slices and binds RNA for use with the Zymoclean Gel RNA Recovery Kit, enabling purification of RNA fragments ≥200 nt from TAE, TBE, MOPS, and formaldehyde-denaturing agarose gels.</p>

RBC Lysis Buffer

<p>RBC Lysis Buffer by Zymo Research selectively lyses red blood cells from anticoagulated whole blood to enrich the leukocyte fraction for RNA extraction — used as the upstream sample preparation step in the Quick-RNA Whole Blood kit workflow. Available in 50 mL and 100 mL formats.</p>