Zymo Reagents

<h2>What are Spin-Column Bacterial and Fungal RNA Extraction Kits?</h2>
&nbsp;
Spin-column bacterial and fungal RNA extraction kits isolate total RNA from Gram-positive bacteria, Gram-negative bacteria, yeasts, filamentous fungi, algae, and protozoa using ultra-high-density BashingBead mechanical cell wall disruption in a chaotropic lysis buffer, followed by RNA binding to a Zymo-Spin silica-membrane column, on-column washing, and elution in 6-100 ul of RNase-free water - all using a standard microcentrifuge. The complete protocol takes under 15 minutes for a batch of 1-12 samples, requiring only a vortex with a microcentrifuge tube adapter, a microcentrifuge, and standard pipetting equipment. No specialized robotics, vacuum manifolds, or magnetic separation racks are needed. Choose a spin-column bacterial/fungal RNA kit for manual extraction of 1-24 samples per run, when existing automation infrastructure does not support magnetic bead formats, or when the fastest possible turnaround from culture to RT-qPCR data is required.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Pinpoint&trade; Slide RNA Isolation System for targeted extraction of total RNA directly from localized tissue sections on microscope slides
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Quick-RNA&trade; Fungal/Bacterial MicroPrep Kit for highly concentrated, low-volume recovery of transcripts utilizing micro-elution spin-column matrices&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
ZR Fungal/Bacterial RNA MiniPrep for precise cell-wall shattering of yeast and recalcitrant spores&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
YeaStar&trade; RNA Kit efficiently permeabilizes tough yeast cell walls.&nbsp;
</li>
</ul>
&nbsp;
<h2>How to Choose a Spin-Column Bacterial/Fungal RNA Kit</h2>
&nbsp;
Miniprep vs. Microprep Format
The Zymo Quick-RNA Fungal/Bacterial Miniprep processes up to 2x10^8 bacteria or 2x10^7 fungi per column, eluting in 25-100 ul - the standard choice for typical 1-5 ml bacterial cultures. The Microprep handles smaller inputs (up to 1x10^7 bacteria) and elutes in as little as 6 ul, concentrating RNA for sensitive applications such as single-cell-equivalent samples or very low-input transcriptomics.
Bead Size for Target Organism
Pre-mixed 0.1 mm + 0.5 mm BashingBead tubes in Zymo kits handle bacteria (0.1 mm) and fungi (0.5 mm) without selecting bead size. For pure Gram-negative bacterial cultures that do not require bead beating, direct lysis in buffer is available as a faster protocol option that skips the bead-beating step entirely.
On-Column DNase I Treatment
Include DNase I for all RT-qPCR and RNA-seq applications. Bacterial gDNA is abundant and co-purifies efficiently under silica-membrane binding conditions; without DNase treatment, gDNA contamination produces false amplification in RT-qPCR assays targeting genes with no introns. DNase I Reaction Mix is included in Zymo Quick-RNA Fungal/Bacterial kits.
Elution Volume
Elute in 25-50 ul for standard RT-qPCR (2-5 ul per reaction provides 5-25 RNA equivalents per assay). For RNA-seq library preparation requiring &gt;=200 ng input, use 50-100 ul elution volume to recover more total RNA mass; then measure concentration by Qubit and adjust for library prep.
Storage of Extracted RNA
Store spin-column bacterial RNA at -80C in single-use aliquots; mRNA from bacteria is AU-rich and degrades rapidly through multiple freeze-thaw cycles. Use within 6 months for transcriptomic applications; RT-qPCR targets with high expression levels remain quantifiable for 12+ months at -80C.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Target quality metrics for spin-column bacterial/fungal RNA: A260/A280 &gt;= 1.8, A260/A230 &gt;= 1.5, and RIN &gt;= 7 from log-phase cultures processed within 60 seconds of harvest. Zymo Research Quick-RNA Fungal/Bacterial kits use the same Zymo-Spin column technology found across the Quick-RNA product line and are validated for inhibitor-free RNA suitable for NGS, RT-PCR, microarray, and hybridization. The Quick-RNA Fungal/Bacterial Miniprep is one of the most widely used spin-column formats for bacterial and fungal RNA in academic molecular microbiology research, with validated protocols for S. cerevisiae, C. albicans, A. fumigatus, and standard Gram-positive and Gram-negative species.&nbsp;
<section class="mbp-contact-cta"></section>
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for kit selection guidance, bulk pricing, and Zymo Research protocol adaptation for non-standard organisms.

Spin-column

Bacterial/Fungal RNA/ Spin-column

MSDS - R1003

MSDS - R1007

Protocol - R1003

Protocol - R1007

R1003-Protocol

Pinpoint™ Slide RNA Isolation System I

Pinpoint Slide RNA Isolation System I (R1003) by Zymo Research — 50-prep RNA isolation from fresh/frozen tissue sections on glass slides. Recovers &ge;17 nt RNA (incl. microRNA), elutes in &ge;6 µL, A260/A280 &gt;1.8, up to 10 µg/prep. All components stored at room temperature.

Equipment

Purity

Sample Source

Size Range

Yield

Manufacturer

Shipping Condition

Storage Condition

Pinpoint™ Slide RNA Isolation System I (50 Preps.)

Pinpoint™ Slide RNA Isolation System II (50 Preps.)

RNA Digestion Buffer (1.2 ml)

RNA Extraction Buffer (12 ml)

RNA Extraction Buffer (3 ml)

RNA Wash Buffer (12 ml)

RNA Wash Buffer (24 ml)

RNA Wash Buffer (48 ml)

RNA Wash Buffer (6 ml)

<h2>What Are Zymo Research Buffers?</h2>
Zymo Research buffers are formulation-specific reagents used at defined steps &mdash; lysis, binding, wash, and elution &mdash; in Zymo-Spin column-based purification protocols for DNA, RNA, and bisulfite-converted DNA. Each buffer is optimized for a specific kit family: DNA Wash Buffer concentrate (requires ethanol addition before use) removes salts and contaminants before elution from Zymo-Spin silica columns; DNA Elution Buffer (10 mM Tris, pH 8.5, 0.1 mM EDTA) releases purified DNA from the matrix; RNA Wash Buffer supports RNA extraction kits including Quick-RNA and Direct-zol lines; M-Elution Buffer is designed for eluting bisulfite-converted DNA in EZ DNA Methylation kits. Researchers typically order these separately when a single buffer component runs out before the rest of the kit, or when scaling up a protocol beyond the volume included.
TL;DR: Order Zymo buffers individually to replenish a specific step in your existing Zymo workflow without replacing the full kit.
<h2>How to Choose Zymo Research Buffers</h2>
<h3>Match the buffer to your kit family</h3>
Every Zymo buffer is paired to a specific kit line. DNA Wash Buffer and DNA/RNA Wash Buffer concentrate are used in DNA Clean &amp; Concentrator, Quick-DNA, and Zymoclean Gel DNA Recovery kits. RNA Wash Buffer pairs with Quick-RNA kits. ZymoBIOMICS DNA Wash Buffer 1 and Wash Buffer 2 are specific to the ZymoBIOMICS microbiomics line. Substituting a buffer from a different kit family can compromise yield or purity.
<h3>Confirm ethanol addition requirements before use</h3>
Several Zymo wash buffer concentrates require addition of 96-100% ethanol to the bottle before first use &mdash; the label states the exact volume. DNA Wash Buffer concentrate, DNA/RNA Wash Buffer concentrate, and Urine DNA Wash Buffer concentrate all require this step. Pre-made (non-concentrate) versions such as RNA Wash Buffer are supplied ready to use.
<h3>Select the right volume for your throughput</h3>
Zymo buffers are available in sizes from 6 ml (single-experiment replenishment) up to 500 ml (Buffer P1 Red, for high-volume plasmid prep labs). For labs processing more than 50 samples per week, ordering the largest available size reduces per-sample cost and the frequency of reordering.
<h3>Choose elution buffer chemistry based on downstream application</h3>
DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA) is the standard for most PCR and sequencing applications. For applications sensitive to EDTA &mdash; such as some restriction enzyme digests or direct use with divalent cation-dependent enzymes &mdash; eluting in molecular-grade water or a Tris-only buffer is preferable. M-Elution Buffer is specifically formulated for bisulfite-converted DNA and should not be substituted with standard elution buffer in methylation workflows.
<h3>Verify research-use-only status</h3>
All Zymo Research buffers sold through MBP are for research use only (RUO). They are not cleared for in vitro diagnostic use. For clinical or diagnostic laboratory applications, confirm regulatory status with your institutional compliance officer before use.
<h2>Compare Zymo Buffer Types</h2>
<table>
<thead>
<tr>
<th>Buffer Type</th>
<th>Primary Application</th>
<th>Kit Family</th>
<th>Format Available</th>
</tr>
</thead>
<tbody>
<tr>
<td>DNA Wash Buffer (concentrate)</td>
<td>Salt removal before DNA elution</td>
<td>DNA Clean &amp; Concentrator, Quick-DNA</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA/RNA Wash Buffer (concentrate)</td>
<td>Salt removal for dual DNA/RNA preps</td>
<td>Quick-DNA/RNA kits</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA)</td>
<td>DNA release from silica column</td>
<td>All Zymo DNA purification</td>
<td>16 ml &ndash; available sizes vary</td>
</tr>
<tr>
<td>RNA Wash Buffer</td>
<td>RNA purification wash step</td>
<td>Quick-RNA kits</td>
<td>Ready-to-use</td>
</tr>
<tr>
<td>M-Elution Buffer</td>
<td>Elution of bisulfite-converted DNA</td>
<td>EZ DNA Methylation kit families</td>
<td>Small volume</td>
</tr>
<tr>
<td>ZymoBIOMICS DNA Wash Buffer 2</td>
<td>Metagenomic/microbiomic DNA cleanup</td>
<td>ZymoBIOMICS DNA kits</td>
<td>Sold separately</td>
</tr>
<tr>
<td>Buffer P1 Red</td>
<td>Alkaline lysis resuspension (plasmid prep)</td>
<td>Zyppy Plasmid Miniprep</td>
<td>10 ml &ndash; 500 ml</td>
</tr>
</tbody>
</table>
<h2>Specifications Context</h2>
Zymo wash buffer concentrates are supplied at a defined fold-concentration (commonly 4x or 6x) and require ethanol addition to reach working strength &mdash; the exact ratio is printed on the bottle and in the kit protocol. Elution buffer volumes for Zymo-Spin columns typically range from 6 to 100 &micro;l per elution; larger elution volumes improve total yield at the cost of DNA concentration. For DNA elution from Zymo-Spin columns, the protocol recommends a minimum 1-minute contact time between elution buffer and the silica matrix to maximize recovery of fragments 10 kb and above. As of 2025, Zymo Research tests all buffer lots for performance consistency, with integrity guaranteed for up to one year from purchase date.
Browse the full <a href="https://www.notion.so/reagents">Zymo Research reagents collection at MBP</a> for kits and columns that use these buffers, or visit our <a href="https://www.notion.so/reagents/buffers">reagent buffers category</a> for non-Zymo buffer options including ABM and other brands. For volume orders or purchase orders, use the <a href="https://www.notion.so/quickorder">Quick Order</a> tool or <a href="https://www.notion.so/contact-us">contact us</a> directly &mdash; MBP is a registered vendor for institutional procurement systems.
Last updated: June 2025
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Zymo Buffers

DataSheet

Plasmid Wash Buffer

D4027-4-12 by Zymo Research is a 12 ml Plasmid Wash Buffer concentrate for Zymo-Spin column wash steps in Zyppy and ZR Plasmid Miniprep workflows. Prepare with 95–100% ethanol before use; stored at room temperature. Sold as a standalone refill when the wash buffer component is depleted.

Plasmid Wash Buffer (Concentrate) (12 ml)

Plasmid Wash Buffer (Concentrate) (24 ml)

Plasmid Wash Buffer (Concentrate) (48 ml)

Plasmid Wash Buffer (Concentrate) (6 ml)

<h2>What Are Nucleases?</h2>
&nbsp;
Nucleases are enzymes that cleave phosphodiester bonds between nucleotide subunits in nucleic acids. Deoxyribonucleases (DNases) act on DNA; ribonucleases (RNases) act on RNA; and some nucleases (S1 Nuclease, Mung Bean Nuclease) act on both single-stranded DNA and RNA. Nucleases are further divided into endonucleases, which cleave internal phosphodiester bonds, and exonucleases, which progressively remove nucleotides from the 5' or 3' terminus of a strand. Major research nucleases include DNase I (non-specific endonuclease for both ss- and ds-DNA), RNase A (cleaves single-stranded RNA at pyrimidine residues), RNase H (degrades the RNA strand of RNA: DNA hybrids), Exonuclease III (removes nucleotides from 3'-recessed or blunt ends of duplex DNA), S1 Nuclease (degrades single-stranded DNA or RNA), and RNase R (degrades linear RNA but not circular RNA). Match the nuclease to the substrate type (single-stranded vs. double-stranded, DNA vs. RNA), the directionality required (endo vs. exo), and cofactor compatibility with your reaction buffer.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
PureRec RNase A (Recombinant): A ribonuclease free of animal-derived components, designed for the swift elimination of RNA from DNA samples, ensuring no threat of viral contamination.
</li>
<li dir="ltr" aria-level="1">
DNase I Set (RNase-free): A highly effective endonuclease formulated for complete DNA digestion in delicate RNA applications while preserving the integrity of the transcript.
</li>
<li dir="ltr" aria-level="1">
PureRec Duplex-Specific Nuclease (DSN): A unique enzyme designed to equalize high-abundance transcripts, greatly enhancing the identification of rare variants in cDNA libraries.
</li>
<li dir="ltr" aria-level="1">
RNase R: A distinctive 3'-to-5' exoribonuclease that breaks down linear RNA but preserves circular RNA forms, crucial for the identification of circRNA.
</li>
<li dir="ltr" aria-level="1">
RNaseOFF Inhibitor: A strong protein-based barrier that counteracts a wide range of RNases, maintaining the delicate transcriptional environment of your samples.
</li>
</ul>
&nbsp;
<h2>How to Choose Nucleases</h2>
&nbsp;
DNase selection
DNase I (bovine pancreatic, recombinant) non-specifically cleaves both single- and double-stranded DNA in the presence of Mg2+ (random cleavage) or Mn2+ (double-strand cuts at approximately the same position on both strands). Use RNase-free DNase I for RNA preparations to remove genomic DNA contamination. Use Plasmid-Safe ATP-Dependent DNase to selectively degrade linear double-stranded DNA while preserving supercoiled and relaxed circular plasmid DNA. Baseline-ZERO DNase is optimized for the complete removal of trace DNA without damaging RNA.
RNase selection
RNase A cleaves single-stranded RNA at pyrimidine (C and U) residues to produce 3'-pyrimidine nucleotides; it is used to remove RNA from DNA preps, to perform RNase protection assays, and to analyze RNA secondary structure. RNase H degrades the RNA strand of RNA: DNA duplexes and is used after first-strand cDNA synthesis to remove the mRNA template. RNase R (3'-&gt;5' exoribonuclease) degrades all linear RNA without affecting circular RNA, and is used in circular RNA enrichment workflows and to characterize circular RNA splice junctions. Terminator 5'-Phosphate-Dependent Exonuclease (Terminator) digests linear RNA with a 5'-monophosphate, enriching for capped mRNA or circular RNA.
Exonuclease selection
Exonuclease III (Exo III) removes nucleotides from the 3' terminus of duplex DNA and generates single-stranded regions used in unidirectional nested deletions, mapping regulatory elements, and generating single-stranded templates for sequencing. Exonuclease I (Exo I) digests single-stranded DNA in the 3'-&gt;5' direction and is used after PCR to remove unincorporated primers before Sanger sequencing. Lambda Exonuclease removes nucleotides from 5'-phosphorylated duplex DNA in the 5'-&gt;3' direction and is used in isothermal amplification and strand displacement workflows.
Cofactor and inhibitor sensitivity
DNase I requires Mg2+ or Ca2+ for activity and is completely inhibited by EDTA at a 5 mM concentration. RNase A is active across a wide pH range (optimum 7.0-7.5) and is exceptionally stable; inactivation requires proteinase K digestion, phenol-chloroform extraction, or RNase inhibitor protein. RNase H requires Mg2+; RNase R requires Mg2+ and is most active between 37-50 degrees C.
Purity and application grade
For RNA-focused applications, all nucleases used on DNA substrates must be certified RNase-free. For DNA-focused work, DNase-free grades of RNases are required. Confirm lot-specific QC contamination data from the manufacturer before use in sensitive workflows.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
DNase I activity is expressed in Kunitz units: 1 unit increases A260 by 0.001 per minute per mL during DNA hydrolysis at pH 5.0. RNase A activity is expressed as the amount of enzyme hydrolyzing 50% of an RNA substrate under defined conditions; it remains active at a wide range of temperatures and is notoriously difficult to inactivate (requires boiling in SDS, proteinase K digestion, or phenol-chloroform extraction). RNase R from E. coli degrades linear RNA processively in the 3'-&gt;5' direction and is used at 2-10 U per microgram of total RNA to enrich for circular RNA species. RNase R treatment before library construction is the standard method for circular RNA sequencing (circRNA-seq) workflows on both short-read and long-read platforms. For protecting RNA from RNase contamination during nuclease-adjacent workflows, see&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/enzymes-and-inhibitors">RNase inhibitors</a>&nbsp;in the parent Enzymes and Inhibitors category.
&nbsp;
Safeguard your molecular images&mdash;<a href="https://mbpinc.net/contact-us">reach out to the MBP team</a> now to obtain a quote for our sophisticated nucleases and inhibitors.

Nucleases

MSDS

Protocol

PureRec Duplex-Specific Nuclease(Liquid)

E1020-200 by Zymo Research is PureRec Duplex-Specific Nuclease (DSN), 200&nbsp;U at 1&nbsp;U/&micro;l (200&nbsp;&micro;l, liquid). Selectively digests dsDNA and DNA-RNA hybrids; spares ssDNA and RNA. Purity &gt;95%, RNase-free. Optimal at 60&deg;C. Also available as E1020-1000.

Unit Definition

Concentration

Optimal Temperature

Reaction Buffer

Stop Solution

Inactivation

PureRec Duplex-Specific Nuclease (1 U/µl, 200 µl) (Liquid)

PureRec Duplex-Specific Nuclease (1 U/ul, 1000 ul) (Liquid)

PureRec RNase A (Recombinant)

PureRec RNase A Recombinant (E1019-5) by Zymo Research — 5 mg at 1 mg/ml, tag-free recombinant RNase A. DNase-free, protease-free, and animal-free. Active across a wide range of buffer conditions. For RNA removal from DNA and protein samples, NGS, and cloning workflows. Made in the USA.

PureRec RNase A (0.1 mg, 100 µl) (Liquid)

PureRec RNase A (5 mg, 1 mg/ml (Liquid)

PureRec RNase A (20 mg, 1 mg/ml) (Liquid)

<h2>What are 5-hmC ELISA kits?</h2>
&nbsp;
5-hmC ELISA kits are antibody-based assays formulated specifically to quantify 5-hydroxymethylcytosine, the oxidized derivative of 5-methylcytosine produced by TET family enzymes, in a genomic DNA sample. The assay typically uses denatured single-stranded DNA coated on a plate well, recognized by an anti-5-hmC primary antibody and developed with an HRP-conjugated secondary antibody for a colorimetric readout proportional to the global level of 5-hmC in the sample.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">DNA hydroxymethylation ELISA assay kits for 5-hmC levels quantification</li>
<li dir="ltr" role="presentation">DNA methylation ELISA kits for comparative gene regulation analysis and measurement of 5-mC&nbsp;</li>
<li dir="ltr" role="presentation">DNA standards for accurate methylation and hydroxymethylation quantification&nbsp;</li>
<li dir="ltr" role="presentation">Control DNA sets and assay controls for experimental accuracy, validation, and reproducibility</li>
<li dir="ltr" role="presentation">HRP-based antibodies for the specific detection in ELISA workflows</li>
<li dir="ltr" role="presentation">ELISA coating and reaction buffers for enhanced assay performance and setup</li>
<li dir="ltr" role="presentation">Signal development reagents for quantitative readout and chemiluminescent or colorimetric detection</li>
</ul>
&nbsp;
<h2>How to use 5-hmC ELISA kits</h2>
&nbsp;
Confirm 5-hmC is detectable at relevant levels in your cell type or tissue
5-hmC is most abundant in neurons and embryonic stem cells, where it can constitute up to 20% of total modified cytosines in neuronal DNA, while most cultured cell lines have much lower 5-hmC levels that may fall below a plate-based antibody assay's reliable detection limit. Checking published 5-hmC abundance for your specific cell type helps confirm whether ELISA-level detection is appropriate before committing samples to the assay.
Use a set of mixed methylation standards to build a calibration curve
Running defined mixtures of 5-hmC-containing and unmethylated DNA at known ratios generates a standard curve that lets you convert the raw absorbance signal to an actual global hydroxymethylation percentage, since the assay signal alone cannot be interpreted as an absolute level without this calibration.
Confirm your antibody's specificity for 5-hmC versus 5-mC
Anti-5-hmC antibodies used in these kits are selected for specificity to 5-hydroxymethylcytosine rather than 5-methylcytosine, but published cross-reactivity data for the specific antibody in your kit is worth checking, particularly when studying sample types where both marks are present at similar levels.
Plan your experimental design to include appropriate negative and positive controls
Including a negative control (unmethylated DNA, expected to give background-level signal) and a positive control (5-hmC-containing DNA of known level) in every plate confirms the assay is performing as expected and catches plate-to-plate variability before it affects result interpretation.
Understand ELISA gives global level, not locus-specific 5-hmC information
A 5-hmC ELISA measures the average hydroxymethylation level across all DNA in the sample; it does not reveal which specific CpG sites carry 5-hmC, which requires hMeDIP-seq or TAB-seq for regional or base-level resolution respectively.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Anti-5-hmC antibodies used in ELISA formats are validated specifically for 5-hydroxymethylcytosine and selected for minimal cross-reactivity with 5-methylcytosine, since distinguishing these two modifications is the primary purpose of a dedicated 5-hmC assay rather than a general methylation assay. As of 2026, 5-hmC ELISA kits remain a standard first-pass tool in studies where many samples need to be screened for differences in global hydroxymethylation level before committing a smaller subset to high-resolution sequencing methods.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at Molecular Biology Products Inc. (MBP) and find reliable ELISA kits and reagents for your lab.

ELISA Kits & Reagents

Quest 5-hmC™ DNA ELISA Kit

The Quest 5-hmC™ DNA ELISA Kit (D5425) by Zymo Research quantifies global 5-hydroxymethylcytosine in 20–200 ng of genomic DNA per well with a sensitivity of ≥0.02% 5-hmC per 100 ng input. The 96-well colorimetric assay completes in 3 hours and is compatible with vertebrate, plant, and microbial DNA, as well as enzyme-digested and sheared fragments.

Detection

Input

Quest 5-hmC™ DNA ELISA Kit (96 Preps.)

Quest 5-hmC™ DNA ELISA Kit (2 x 96 Preps.)

<h2>What Are SARS-CoV-2 RT-qPCR Detection Kits?</h2>
&nbsp;
SARS-CoV-2 RT-qPCR detection kits provide the reverse transcriptase, hot-start Taq polymerase, buffer, and primer/probe sets needed to detect SARS-CoV-2 viral RNA from respiratory specimens by one-step RT-qPCR. Most kits target conserved regions of the viral genome, such as the N (nucleocapsid) gene, alongside a human RNase P (RP) control to confirm sample adequacy.&nbsp;Choose an extraction-based RT-qPCR kit for maximum sensitivity in clinical-style research workflows; choose a kit validated for direct (extraction-free) use when RNA extraction reagent availability or turnaround time is the limiting factor.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>rRT-PCR Detection Kits: Established tools such as the Quick SARS-CoV-2 rRT-PCR Kit, made for specific amplification and dependable detection of viral sequences in both clinical and research specimens.</li>
<li>Multiplex Kits: Efficient multiplexing options that enable the concurrent identification of several viral targets within one reaction to enhance laboratory productivity.</li>
<li>Reverse Transcription Pre-Mixes: Enhanced blends such as ZymoScript RT Pre-Mix, uniquely designed to facilitate the transformation of viral RNA into stable cDNA for further examination.</li>
</ul>
&nbsp;
<h2>How to Choose a SARS-CoV-2 Detection Kit</h2>
&nbsp;
Extraction-Based vs. Extraction-Free Workflows
Standard RT-qPCR workflows use purified viral RNA from an extraction kit as input. Extraction-free (direct) RT-qPCR workflows instead use heat-treated specimen diluent directly as input, avoiding the extraction step entirely. A heat treatment step (commonly 65&deg;C for approximately 10 minutes) lyses virions and inactivates RNases sufficiently for many one-step RT-qPCR master mixes to detect the released RNA.
Master Mix Performance on Direct Samples
Not all one-step RT-qPCR master mixes perform equally on unextracted, heat-treated specimens. Comparative studies have found that some master mix formulations provide substantially higher analytical sensitivity than others when used directly on heat-treated nasopharyngeal swab diluent. Confirm that the specific master mix has been validated for direct-sample use if extraction-free testing is the goal.
Target Gene and Control Design
Kits commonly target a conserved viral gene, such as N1/N2 regions of the nucleocapsid gene used in CDC-style primer/probe sets, alongside a human RNase P control to confirm adequate sample collection and absence of PCR inhibition. Multiplexed detection of both targets in one reaction is standard.
Sample Type Compatibility
Some kits are specifically formulated for unpurified saliva samples, enabling a streamlined extraction-free protocol completed in under an hour. Others are validated primarily for nasopharyngeal swab specimens in viral transport medium. Confirm that sample type compatibility matches your intended specimen source.
Analytical Sensitivity and Validation Data
Published validation studies report limits of detection in the range of approximately 103-104 copies/mL for optimized direct RT-qPCR protocols, with sensitivity, specificity, and accuracy figures commonly reported at 95%+ sensitivity and 98%+ accuracy relative to standard extraction-based methods. Review available validation data when selecting a kit for a specific application.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
In one published evaluation, incubating nasopharyngeal specimens at 65&deg;C for 10 minutes and testing the resulting supernatant directly with a one-step RT-qPCR master mix achieved a limit of detection of approximately 6.6 &times; 103 copies/mL with high reproducibility, and sensitivity, specificity, and accuracy of approximately 95%, 99%, and 98.5%, respectively, relative to a standard RNA extraction-based approach across a panel of clinical specimens.&nbsp;Not all master mix formulations performed equivalently in this direct format. Formulation choice was the dominant variable affecting sensitivity when extraction was omitted. Multiplexed N gene plus human RNase P primer/probe sets, consistent with CDC-style assay design, allow simultaneous target detection and sample adequacy control in one reaction.&nbsp;SARS-CoV-2 RT-qPCR kits are typically stored at -20&deg;C to -30&deg;C and protected from light. Lot-specific Certificates of Analysis (CoAs) are available on request. These products are intended for research use.
&nbsp;
Ensure your diagnostic precision&mdash;<a href="https://mbpinc.net/contact-us">reach out to the MBP team</a> now for a quote on our expert SARS-CoV-2 PCR services.

SARS-CoV-2 Kits

SARS-CoV-2

MSDS (EU)

MSDS (US)

Quick SARS-CoV-2 Multiplex Kit

R3013 by Zymo Research is a multiplex rRT-PCR kit detecting SARS-CoV-2 N gene (HEX) and human RNase P (Quasar 670) simultaneously in a single well from purified respiratory RNA in 2 hours or less. Limit of detection is 167 GEC/ml (10 GEC/reaction). CE-IVD marked. Industry scientists and clinical laboratory professionals benefit from the dual-channel multiplexing format, 384-well scalability, and DNA/RNA Shield-compatible workflow.

Compatibility

Equipment Required

Input Quality

Processing Time

Reagents

Registration Status

Quick SARS-CoV-2 Multiplex Kit (100 Rxns)

Quick SARS-CoV-2 Multiplex Kit (10,000 Rxns)

Quick SARS-CoV-2 Multiplex Kit (1000 Rxns)

Instructions for Use

Healthcare Provider Fact Sheet

Patient Fact Sheet

KingFisher Script

Quick SARS-CoV-2 rRT-PCR Kit

R3011 by Zymo Research is an rRT-PCR kit detecting SARS-CoV-2 viral RNA and human RNase P simultaneously from purified respiratory RNA in 1.5 hours or less. Limit of detection is 250 GEC/ml (15 GEC/reaction). CE-IVD marked for use in the EU. Industry scientists and clinical laboratory professionals running high-throughput SARS-CoV-2 molecular testing benefit from the ready-to-use master mix format and dual-target confirmation design.

Sample Input Material

Quick SARS-CoV-2 rRT-PCR Kit (100 Rxns)

Quick SARS-CoV-2 rRT-PCR Kit (10,000 Rxns)

Quick SARS-CoV-2 rRT-PCR Kit (1000 Rxns)

<h2>What are 16S/ITS NGS library preparation kits?</h2>
&nbsp;
16S/ITS NGS library preparation kits convert extracted microbial DNA into a sequencing-ready library by amplifying a targeted hypervariable region: the 16S ribosomal RNA gene for bacterial and archaeal community profiling, or the ITS (internal transcribed spacer) region for fungal community profiling. Both genes contain conserved flanking sequences that serve as universal PCR primer binding sites, allowing amplification of the variable region in between, which is then sequenced and compared against a reference database for taxonomic classification.
&nbsp;
<h2 dir="ltr">What you will discover:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Quick-16S&trade; Plus Kits (V1&ndash;V4): Enhanced systems for targeted amplification of bacterial 16S rRNA, incorporating validated reagents for accurate classification.
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Quick-ITS&trade; Plus Library Prep: Sensitive kits for fungal detection, employing specialized chemistry to capture ITS regions while preserving genomic integrity at its best.
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UDI Indexing Integration: Sophisticated indexing methods to avoid barcode hopping and guarantee precise demultiplexing during simultaneous processing.
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Accelerated Workflows: Nuclease-free procedures that reduce manual effort while ensuring transfection-grade purity for delicate sequencing systems.
</li>
</ul>
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<h2>How to choose a 16S/ITS library preparation kit</h2>
&nbsp;
Confirm which hypervariable region(s) your kit targets
16S library prep kits commonly target specific hypervariable regions such as V3-V4, and primer sets re-designed against an updated 16S reference database can meaningfully improve taxonomic coverage compared with older primer designs that may underrepresent certain taxa.
Decide between single-region and full-length amplicon coverage
Short-read sequencing of a single hypervariable region, or a small number of regions, is the standard, cost-effective approach, while long-read platforms can sequence the full-length 16S gene, providing improved species- and strain-level resolution at a different cost and throughput tradeoff.
Plan for PCR chimera formation as a known amplicon sequencing challenge
PCR chimeras, artificial sequences formed from recombination of multiple templates during amplification, are a recognized challenge in amplicon library prep, and kits using real-time PCR to control amplification cycle number can help keep chimera abundance to a low, well-controlled percentage of total reads.
Confirm fungal ITS coverage if your study includes fungal community profiling
Some 16S kits can be run alongside a separate ITS-targeted protocol, or as a combined workflow, to capture both bacterial and fungal community composition from the same sample set, which matters for studies where fungi are a relevant part of the community.
Check hands-on time and workflow complexity for your sample throughput
Some 16S library prep kits are designed for a single-tube, single-reaction workflow with simultaneous indexing and amplification, substantially reducing hands-on time and contamination risk compared with multi-step protocols, which matters for higher-throughput sample sets.
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<h2>Specifications context</h2>
&nbsp;
16S rRNA sequencing requires less input DNA and is less impacted by the presence of host DNA than shotgun metagenomic sequencing, which is part of why it remains the more accessible default for routine bacterial community composition profiling across many labs and sample types. As of 2026, single-tube, real-time-PCR-controlled 16S library prep workflows continue to reduce hands-on time for high-throughput studies while keeping PCR chimera formation to a low, well-controlled percentage of total reads.
<a href="https://mbpinc.net/contact-us">Reach out to the MBP Inc. team</a> today to inquire about a quote for your NGS library preparation requirements.

16S/ITS NGS Library Preparation Kits

Quick-16S Plus NGS Library Prep Kit (V1-V2)

Quick-16S™ Plus NGS Library Prep Kit V1-V2 (D6434-PS1) by Zymo Research — 96-reaction single-PCR kit targeting the V1-V2 region with 30 min hands-on time. Compatible with Illumina MiSeq and NextSeq 1000/2000. No normalization required.

16s V1-V2 Primer Sequences (adapters not included)

Amplicon Size

Barcode Sequences

Index Primers

Required Equipment

Sample Input

Sequencing Platform

Quick-16S Plus NGS Library Prep Kit (V1-V2) with Primer Set 1

Quick-16S Plus NGS Library Prep Kit (V1-V3) with Primer Set 1

Guide for Preparing More Than 96 Samples

Plate Setup Guide

Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI)

The D6421-PS1 by Zymo Research is a 96-reaction Quick-16S Plus NGS Library Prep Kit for V3-V4 16S rRNA amplicon sequencing, using a single qPCR/PCR step with Unique Dual Index (UDI) 10-bp barcodes to prepare 96 samples in ~30 minutes hands-on time, with no normalization or gel analysis required. Primer Set 1 (341f/806r) covers 84.2% bacteria and 75.8% archaea; compatible with Illumina MiSeq and NextSeq 2000. Made in the USA.

16S V3-V4 Primer Sequences (adapters not included)

Primer Coverage

Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI) with Primer Set 1

Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI) with Primer Set 2

Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI) with Primer Set 3

Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI) with Primer Set 4

Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI) with Primer Set 5

Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI) with Primer Set 6

Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI) with Primer Set 7

Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI) with Primer Set 8

Protocol - D6430

Protocol - D6432

Guide for Preparing More than 96 Samples

Quick-16S Plus NGS Library Prep Kit (V4)

D6430-PS1 Quick-16S Plus NGS Library Prep Kit (V4) by Zymo Research prepares 96 indexed Illumina-compatible 16S libraries targeting the V4 region (515–806) using EMP primers, with 30 min hands-on time and MagBead cleanup.

Equipment Needed (user provided)

Index Sequences

V4 Primer Sequences (adapters not included)

Quick-16S Plus NGS Library Prep Kit (V4) (96 preps) with Index Primer Set 1

Quick-16S Plus NGS Library Prep Kit (V4) (96 preps) with Index Primer Set 2

Quick-16S Plus NGS Library Prep Kit (V4) (96 preps) with Index Primer Set 3

Quick-16S Plus NGS Library Prep Kit (V4) (96 preps) with Index Primer Set 4

Quick Protocol -  High Microbial

Quick Protocol - Low Microbial

Quick Protocol - Non-Quant PCR

Guide For > 96 Samples

Quick-16S™ NGS Library Prep Kit

D6400 by Zymo Research converts purified microbial DNA from up to 96 samples into Illumina MiSeq-ready 16S amplicon libraries targeting V1–V2 (~486 bp) or V3–V4 (~596 bp) hypervariable regions. Input is 2 µL of DNA at 5–20 ng/µL; dual barcoding supports 96-plex sequencing. Compatible with standard and low-biomass microbiome protocols.

Preparing > 96 Samples

Recommended RT PCR System

Sequencing Compatibility

Quick-16S™ NGS Library Prep Kit (96 Rxns.)

Quick-16S™ Primer Set V1-V2 (400 µl)

Quick-16S™ Primer Set V3-V4 (400 µl)

<h2>What Are 96-Well Plate Genomic DNA Kits for Blood and Tissue?</h2>
&nbsp;
96-well plate genomic DNA kits for blood and tissue replace individual spin columns with silica-binding or glass-fiber plates that enable simultaneous purification of genomic, mitochondrial, or pathogen DNA from up to 96 samples per run. These systems are designed for whole blood, serum, plasma, lymphocytes, cultured cells, and selected tissue samples, providing efficient high-throughput DNA extraction for research and molecular testing workflows.&nbsp;Choose a 96-well plate genomic DNA kit when sample volumes justify batch processing on a plate-compatible centrifuge or vacuum manifold.
&nbsp;
<h2>What You Will Find</h2>
&nbsp;
<ul>
<li>Streamlined high-throughput processing using 96-well plate formats for simultaneous genomic DNA purification.</li>
<li>High-purity DNA extraction from blood and tissue samples using optimized purification chemistries.</li>
<li>Compatibility with both automated and manual workflows for scalable sample preparation in research and diagnostic laboratories.</li>
<li>Purified DNA suitable for sequencing, PCR, qPCR, genotyping, and other downstream molecular biology applications.</li>
</ul>
<h2>&nbsp;</h2>
<h2>How to Choose a 96-Well Plate Blood and Tissue Genomic DNA Kit</h2>
&nbsp;
Sample Input and Well Capacity
Most kits accept up to 200 &micro;L of whole blood, serum, plasma, or approximately 5 &times; 106 cultured cells per well. Exceeding recommended input volumes can reduce DNA binding efficiency and overall recovery.
Centrifuge Rotor vs. Vacuum Manifold Compatibility
96-well plate kits typically require either a centrifuge equipped with a compatible 96-well plate rotor and deep bucket carriers or a vacuum manifold system. If your laboratory lacks a plate-compatible centrifuge, confirm vacuum manifold compatibility before selecting a kit.
Yield and Elution Volume
A typical extraction yields approximately 6 &micro;g of genomic DNA from 200 &micro;L of whole blood, with elution volumes commonly around 200 &micro;L using TE buffer or nuclease-free water. Processing times generally range from 45 minutes to a few hours, depending on workflow design.
Throughput and Sample Type Considerations
These kits are optimized for high-throughput processing and can handle large sample batches efficiently. When processing multiple sample types within the same plate, additional pretreatment steps may be required for certain tissues or cell types.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The key specifications for 96-well plate genomic DNA extraction kits include sample input volume, DNA yield, elution volume, and compatibility with centrifuge-based or vacuum-manifold workflows. Most kits in this category are designed for high-throughput purification of genomic DNA from blood, tissue, and cultured cell samples while maintaining consistent recovery across large sample batches.&nbsp;Products such as the Quick-DNA 96 Kit, Quick-DNA 96 Plus Kit, 96-well Blood DNA Kit, and 96-well Tissue DNA Kit support parallel processing in a plate-based format, helping laboratories scale routine genotyping, PCR, qPCR, and sequencing workflows. Typical workflows combine optimized lysis and silica-based DNA binding chemistry to remove inhibitors while producing purified DNA suitable for downstream molecular biology applications.&nbsp;When selecting a kit, confirm compatibility with your laboratory's centrifuge rotor, 96-well carrier, or vacuum manifold system, as equipment requirements vary between platforms. Most kit components are stored at room temperature, while any included enzyme reagents should be stored according to the manufacturer's recommendations. Lot-specific Certificates of Analysis (CoAs) are available upon request.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the MBP team </a>today to discuss high-throughput genomic DNA extraction workflows and receive expert guidance on selecting the right 96-well plate solution for your laboratory.

96-well plate

Quick-DNA 96 Kit

D3010 Quick-DNA 96 Kit by Zymo Research purifies inhibitor-free genomic DNA (≤5 µg/well, ≥40 kb) from whole blood, cells, and tissue in a 2×96-well plate format. Compatible with PCR, genotyping, bisulfite conversion, and NGS.