Zymo Reagents

<h2>What are ChIP buffers?</h2>
&nbsp;
ChIP buffers are the aqueous formulations used at each stage of a chromatin immunoprecipitation protocol: cell lysis buffer disrupts the plasma membrane and releases nuclear content, nuclear lysis buffer solubilizes chromatin under conditions compatible with subsequent immunoprecipitation, sonication buffer maintains chromatin stability during shearing, ChIP dilution buffer reduces SDS concentration from the lysis buffer to allow antibody-antigen binding, sequential wash buffers with increasing ionic strength and detergent remove non-specifically bound chromatin while preserving the specific antibody-antigen-DNA complex, and elution buffer releases the captured complex from the antibody-bead resin before de-crosslinking.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">MN Digestion Buffer used in MNase-based workflows for enzymatic fragmentation of&nbsp; chromatin&nbsp;</li>
<li dir="ltr" role="presentation">Chromatin Shearing Buffer using sonification for chromatin disruption and preparation</li>
<li dir="ltr" role="presentation">5X MN Stop Buffer for the termination of enzymatic digestion reactions</li>
<li dir="ltr" role="presentation">Chromatin Dilution Buffer used in ChIP assays to provide optimized conditions for antibody binding&nbsp;</li>
<li dir="ltr" role="presentation">Chromatin Wash Buffer I, II, and III for background reduction and step-by-step non-specific binding removal&nbsp;</li>
<li dir="ltr" role="presentation">ChIP DNA Binding Buffer for purification and capture of immunoprecipitated DNA</li>
<li dir="ltr" role="presentation">5X Chromatin Elution Buffer for protein&ndash;DNA complexes recovery</li>
<li dir="ltr" role="presentation">5M NaCl for enhancing elution and chromatin stabilization</li>
<li dir="ltr" role="presentation">Nuclei Prep Buffer used before chromatin extraction for superior nuclear isolation</li>
</ul>
&nbsp;
<h2>How to use ChIP buffers</h2>
&nbsp;
Always add protease inhibitors to lysis and wash buffers immediately before use
Protease inhibitors must be added fresh to cell lysis and nuclear lysis buffers immediately before use, since most inhibitors are unstable in aqueous solution and lose activity quickly, and without them, nuclear proteases released during lysis will degrade histone tails and reduce ChIP enrichment of histone modifications.
Confirm SDS concentration is diluted before immunoprecipitation
High SDS in the nuclear lysis buffer is necessary for chromatin solubilization and sonication, but must be diluted approximately 10-fold using ChIP dilution buffer before antibody addition, since high SDS disrupts antibody-antigen binding and reduces immunoprecipitation efficiency.
Keep all buffers and chromatin on ice throughout the workflow after lysis
Chromatin prepared for ChIP is sensitive to non-specific interactions and protease activity after cell lysis, and maintaining samples at 4&deg;C throughout all lysis, sonication, immunoprecipitation, and wash steps minimizes these effects and preserves specificity of the enrichment.
Optimize wash buffer stringency to balance specificity against yield
ChIP wash buffers are applied sequentially at increasing ionic strength and detergent concentration to remove non-specifically bound chromatin; conditions too stringent can also strip the specific antibody-antigen-DNA complex from the beads, reducing yield, so optimization of wash conditions for each antibody-target combination improves performance.
Confirm elution buffer composition fits your downstream application
Standard ChIP elution buffers containing SDS and sodium bicarbonate release the protein-DNA complex and reverse crosslinks, but high SDS in the eluate can inhibit some NGS library preparation enzymes, making a DNA cleanup step after elution important before proceeding to library prep.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Protease inhibitor cocktails, added fresh to lysis buffers immediately before ChIP, are a standard component across kit formats and in-house protocols, since even brief protease activity can compromise histone modification detection by degrading the histone tails that ChIP antibodies recognize. As of 2026, ChIP buffer formulations compatible with low-input chromatin and NGS library preparation continue to be optimized for primary cell and tissue ChIP workflows.
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP and request a quote today to get high-quality ChIP buffers for your lab.

Buffers

MSDS

Nuclei Prep Buffer

D5220-2 by Zymo Research is Nuclei Prep Buffer for chromatin immunoprecipitation (ChIP). Used at 500 µl (with protease inhibitors) to resuspend cross-linked cell pellets before chromatin shearing. Compatible with Zymo-Spin ChIP Kit (D5209, D5210). Store at room temperature. Available in 30 mL and 50 mL.

Manufacturer

Shipping Condition

Storage Condition

Nuclei Prep Buffer (50 ml)

Nuclei Prep Buffer (30 ml)

<h2>What are PCR Cleanup Kits?</h2>
&nbsp;
PCR cleanup kits remove residual PCR reaction components -- primers (&lt;100 bp), primer dimers, unincorporated dNTPs, DNA polymerase, buffer salts, and mineral oil - from PCR amplification products using silica-membrane spin columns and a bind-wash-elute protocol. The purified PCR amplicon (&gt;=100 bp) is recovered in 20-50 ul of water or elution buffer, free of inhibitors that would interfere with downstream steps, including Sanger sequencing, restriction enzyme digestion, ligation, NGS library preparation, and in vitro transcription. The procedure takes 5-10 minutes using a standard microcentrifuge with no organic solvents, precipitation, or centrifugation spins beyond the bind/wash/elute steps. Use a PCR cleanup kit immediately after PCR whenever the downstream application requires an inhibitor-free amplicon - primers and excess dNTPs left in the reaction will reduce sequencing quality, compete in ligation reactions, and inflate NGS background noise.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Oligo Clean &amp; Concentrator: Precisely designed systems for the quick retrieval of single-stranded DNA, RNA, and oligonucleotides from enzymatic processes.
</li>
<li dir="ltr" aria-level="1">
OneStep&trade; PCR Inhibitor Removal: Tailored columns and 96-well formats engineered to eliminate polyphenols, humic acids, and lipids that hinder PCR kinetics.
</li>
<li dir="ltr" aria-level="1">
ssDNA/RNA Clean &amp; Concentrator&trade;: An adaptable method for purifying and concentrating small nucleic acid segments, ensuring isoform specificity.
</li>
<li dir="ltr" aria-level="1">
ZR-96 High-Throughput Systems: Scalable formats of 96-well plates designed for automated lab processes, guaranteeing reproducibility-validated outcomes across extensive sample groups.
</li>
</ul>
&nbsp;
<h2>How to Choose a PCR Cleanup Kit</h2>
&nbsp;
Binding Capacity
Standard PCR cleanup columns bind up to 5-25 ug of DNA, depending on the kit; 25 ug capacity (Thermo Fisher GeneJET) accommodates typical preparative PCR reactions. For high-yield PCR reactions producing &gt;25 ug of amplicon, split the reaction across two columns and pool the eluates, or use a higher-capacity kit format.
Fragment Size Range
Standard PCR cleanup kits retain fragments &gt;=100 bp and discard everything shorter. For amplicons between 70 and 100 bp (e.g., short RT-qPCR products or miRNA amplicons), use a kit with an extended lower size limit -- the Thermo Fisher GeneJET kit retains fragments from 25 bp to 20 kb, covering this range. For amplicons shorter than 25 bp, conventional silica-column cleanup will not work; use gel purification or size-selective bead cleanup (e.g., AMPure XP at adjusted bead ratios).
Minimum Elution Volume
If the downstream application requires a high DNA concentration (Sanger sequencing needs &gt;20 ng/ul input), select a kit that elutes in as little as 20-25 ul without significant yield loss.&nbsp;
pH Indicator
Kits include a pH indicator dye in the binding buffer that turns yellow when the pH is correct for DNA binding and remains orange/red if the pH is too high (causing DNA loss). This is useful when purifying PCR products amplified in high-pH buffers. Kits without pH indicators require careful attention to binding buffer volume ratios to ensure efficient DNA adsorption.
96-Well Throughput
For screening workflows producing multiple amplicons per sample, use 96-well PCR purification plates with vacuum manifolds for simultaneous processing of all 96 wells. 96-well formats are essential for high-throughput genotyping and sequencing library preparation pipelines.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Key benchmarks for PCR cleanup kits: &gt;=70% DNA recovery for fragments 100 bp-10 kb, A260/A280 &gt;= 1.7 in eluate, complete removal of primers and dNTPs (confirmed by capillary electrophoresis with no peaks below 100 bp), and elution in &lt;=50 ul of water or TE. The Thermo Scientific GeneJET PCR Purification Kit specifies recovery rates up to 100% with a binding capacity of 25 ug per column in a 5-minute protocol. AAT Bioquest study comparing commercial PCR cleanup kit performance confirmed that silica-column kits consistently achieve primer and dimer removal equivalent to ethanol precipitation with significantly reduced protocol time. PCR cleanup is a mandatory step before NGS library preparation in nearly all sequencing-based workflows at institutions, including Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center, where primer and dimer contamination in libraries produces low-complexity sequencing reads that reduce effective coverage.
&nbsp;
Purify your amplicons and remove reaction noise&mdash;<a href="https://mbpinc.net/contact-us">reach out to MBP Inc.</a> now to obtain a quote for your PCR cleanup process.

PCR Cleanup Kits

D4060-1-Data Sheet

D4060 - MSDS

D4061 - MSDS

D4060-1 - MSDS

D4060-1-Protocol

Oligo Clean & Concentrator

D4060-1 by Zymo Research is the Oligo Clean &amp; Concentrator — a spin-column kit that purifies DNA and RNA oligonucleotides (≥16 nt) in approximately 2 minutes with &gt;90% recovery, eluting in as little as 6 µL for use in hybridization, sequencing, and PCR applications.

Applicable For

Elution Volume

Equipment

Purity

Sample Source

Size Range

Yield

Oligo Clean & Concentrator (50 Preps.)

Oligo Clean & Concentrator (200 Preps.)

Oligo Binding Buffer (10 ml)

Oligo Binding Buffer (40 ml)

Devices & Instruments

One-Way Luer-Lock Clear Stopcock (20 Pack)

The One-Way Luer-Lock Clear Stopcock by Zymo Research (S7001) is a 20-pack of transparent, single-use stopcocks with luer-lock fittings for precise vacuum flow control on the Zymo Research EZ-Vac Vacuum Manifold. Each stopcock delivers individual column control during vacuum-assisted DNA, RNA, and protein purification workflows.

<h2>What are non-bisulfite methylation kits and reagents?</h2>
&nbsp;
Non-bisulfite methylation kits and reagents are alternatives to sodium bisulfite conversion that achieve the same goal &mdash; distinguishing methylated from unmethylated cytosines at single-base or regional resolution &mdash; without the harsh chemical treatment that bisulfite introduces. The most widely adopted approach is enzymatic methyl-seq (EM-seq), which uses TET2 to oxidize 5-methylcytosine and 5-hydroxymethylcytosine and T4-BGT to protect them from subsequent APOBEC3A-mediated deamination, so that only unmodified cytosines are converted to uracil.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">qMethyl PCR assay kit uses PCR amplification for quantitative DNA methylation detection</li>
<li dir="ltr" role="presentation">One-step qMethyl PCR system for efficient methylation analysis and optimized workflow setup&nbsp;</li>
<li dir="ltr" role="presentation">Test reaction premixes for validation and optimization of assays&nbsp;</li>
</ul>
&nbsp;
<h2>How to choose non-bisulfite methylation reagents</h2>
&nbsp;
Choose EM-seq for superior library quality over bisulfite at the same resolution
EM-seq outperforms bisulfite-converted libraries on all major quality metrics: higher library yield, fewer duplicates, more usable reads, more even GC distribution, and accurate methylation calling, without requiring any changes to downstream bioinformatic analysis pipelines since the output is the same C-to-T chemistry as bisulfite conversion.
Understand EM-seq's two-step enzymatic mechanism
In the first EM-seq step, TET2 oxidizes 5-mC and 5-hmC to protected forms while T4-BGT glucosylates 5-hmC; in the second step, APOBEC3A deaminates only the unprotected unmodified cytosines to uracil. The net result is the same as bisulfite conversion &mdash; unmethylated C reads as T &mdash; but without bisulfite's destructive chemistry.
Consider TAPS for mild, nondestructive conversion that directly marks modified cytosines
TET-assisted pyridine borane sequencing (TAPS) is a bisulfite-free method that combines TET oxidation of 5-mC and 5-hmC with pyridine borane reduction, converting modified cytosines to a thymine read while leaving unmodified cytosines intact. Unlike bisulfite and EM-seq, TAPS positively marks the modified bases rather than converting the unmodified ones, and can preserve DNA fragments over 10 kilobases.
Choose MeDIP for genome-wide enrichment without per-cytosine resolution
Methylated DNA immunoprecipitation uses an antibody specific to 5-methylcytosine or 5-hydroxymethylcytosine to pull down methylated DNA fragments, providing regional enrichment data at lower cost and without bisulfite-related DNA damage, though at lower resolution than sequencing-based conversion methods.
Confirm whether your method distinguishes 5-mC from 5-hmC or co-detects both
EM-seq detects both 5-mC and 5-hmC together and cannot distinguish them without additional steps, while TAPS also co-detects both; TAPS&beta;, a variant, can specifically call 5-mC by blocking 5-hmC before oxidation. MeDIP can be targeted specifically to 5-hmC (hMeDIP) using an anti-5-hmC antibody.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
EM-seq was effective using as little as 100 pg of DNA input, and libraries showed higher yields with fewer PCR cycles than bisulfite-converted libraries at all input amounts tested, with more even GC distribution, reduced sequencing duplicates, and more CpGs detected within genomic features per sequencing read. As of 2026, enzymatic conversion via EM-seq is broadly recommended over bisulfite conversion for new methylome analysis projects, particularly those working with limited, degraded, or high-value samples where the DNA damage of bisulfite treatment is most consequential.
This category sits within DNA/RNA Methylation, alongside Bisulfite Conversion Kits, and is closely related to DNA Hydroxymethylation for 5-hmC-specific approaches. See the full reagents catalog and use Quick Order for recurring orders.
<a href="https://mbpinc.net/contact-us" title="Contact the expert team at Molecular Biology Products Inc. (MBP)">Contact the expert team at Molecular Biology Products Inc. (MBP)</a> to find dependable non-bisulfite kits and reagents for your lab.

Non-Bisulfite Kits & Reagents

Protocol

OneSTEP PLUS qMethyl PCR (96 Rxns.)

D5312 by Zymo Research quantifies DNA methylation at user-defined loci without bisulfite conversion, using MSRE-based real-time PCR. Percent methylation is calculated as 100 × 2−ΔCt from 5 ng–1 µg of unmodified genomic DNA per reaction, detecting methylation levels as low as ~1–5%. Compatible with Bio-Rad CFX96 and similar real-time PCR instruments (SYBR Green or TaqMan). Stored at −20°C.

Compatible

Detection Dye

Input

Processing Time

Storage

MSDS - D6035

MSDS - D6035-1-30

Protocol - D6035

OneStep-96™ PCR Inhibitor Removal Kit

OneStep-96™ PCR Inhibitor Removal Kit (D6035) by Zymo Research — 96-well format, 192 preps total. Removes humic/fulvic acids, polyphenols, tannins, and melanin from DNA and RNA extracts in a single step with no centrifugation.

Sample Storage

Type

OneStep-96™ PCR Inhibitor Removal Kit (2 x 96 Preps.)

Prep Solution (30 ml)

MSDS - D6030

MSDS - D6031

Protocol - D6030

Protocol - D6031

OneStep™ PCR Inhibitor Removal Kit

D6030 OneStep PCR Inhibitor Removal Kit by Zymo Research — 50 preps, removes humic acids, tannins, melanin in ~1 min, 50–200 µl input and elution, room-temperature storage.

OneStep™ PCR Inhibitor Removal Kit (50 Preps.)

OneStep™ PCR Inhibitor Removal Kit (Updated) (50 Preps.)

<h2 data-section-id="b4wzzk" data-start="0" data-end="47">What is bacterial culture media for cloning?</h2>
&nbsp;
Bacterial culture media for cloning workflows includes LB (Luria-Bertani) broth and agar, the standard general-purpose media for E. coli growth, SOC media used for post-transformation recovery, and Terrific Broth for higher-density culture in plasmid preparation. Pre-made agar plates with antibiotic selection (ampicillin, kanamycin, chloramphenicol, and others matching common selection markers) are used to isolate colonies carrying the desired plasmid. Related entities include antibiotic selection markers matching transformation kit resistance genes, post-transformation recovery time, and plasmid yield scaling with culture volume and media richness.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">ZymoBroth Growth Media for efficient transformation workflows to generate highly competent bacterial cells</li>
<li dir="ltr" role="presentation">Overexpression Broth (OB) optimized for increasing recombinant protein yields with high-density nutrient media.</li>
<li dir="ltr" role="presentation">Expansion Broth (EB) for the faster and more reliable expansion of microbial cultures.</li>
<li dir="ltr" role="presentation">Dual Media Sets for integrated growth and induction workflows with the help of combined EB and OB systems</li>
</ul>
&nbsp;
<h2 data-section-id="9xi4x5" data-start="919" data-end="971">How to choose bacterial culture media for cloning</h2>
&nbsp;
LB broth/agar vs. Terrific Broth
LB broth and agar are the standard general-purpose media for routine cloning, colony selection, and small-scale plasmid preparation, while Terrific Broth provides higher nutrient density supporting higher bacterial cell density, making it useful when maximizing plasmid yield in larger-scale preparations.
SOC media for post-transformation recovery
SOC media is richer than standard LB and supplemented with glucose and magnesium. It is commonly used for a brief recovery incubation immediately after heat-shock or electroporation transformation, helping transformed cells recover before plating on selective agar. Some transformation protocols specifically recommend SOC rather than LB for this recovery step.
Antibiotic selection matching
Pre-made antibiotic-selective agar plates must match the resistance marker carried by the transformed plasmid, whether ampicillin, kanamycin, chloramphenicol, or another selection marker. Using the wrong antibiotic&mdash;or no antibiotic at all&mdash;will not properly select for plasmid-containing colonies and may allow unwanted bacterial growth.
Liquid vs. agar plate formats and shelf life
Liquid media (broth) is used for post-transformation recovery and culture scale-up, while agar plates are used for colony isolation and selection. Pre-made antibiotic plates have a defined shelf life because antibiotics gradually lose activity over time, making expiration dates particularly important for antibiotics with lower stability.
&nbsp;
<h2 data-section-id="1fljvas" data-start="3523" data-end="3548">Specifications context</h2>
&nbsp;
LB broth and agar are formulated with defined concentrations of tryptone, yeast extract, and sodium chloride, with agar plates commonly supplied in standard Petri dish sizes (for example, 100 mm) and containing antibiotic concentrations matched to common resistance markers, such as 100 &micro;g/mL ampicillin or 50 &micro;g/mL kanamycin. SOC recovery is typically performed as a short incubation, often around one hour at 37&deg;C with shaking, before plating cells on selective agar.&nbsp;
This sub-category is part of MBP's cloning catalog alongside transformation kits and competent cells, the immediately preceding step, and chemicals/reagents for enzyme-based fragment preparation. Labs scaling up plasmid production for lentivirus packaging in immortalization workflows can also review MBP's cell immortalization catalog. MBP's specialist team can help confirm antibiotic concentration and media format requirements for a specific selection marker before order placement.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today to request a quote for high-quality culture media.

Culture Media

Overexpression Broth (OB)

Overexpression Broth OB — M3013-100 by Zymo Research — is a 100 mL specialized bacterial medium for high-level recombinant protein expression, designed for use alongside Expansion Broth (EB) in a two-phase culture protocol. It stores at room temperature and is also available in a 500 mL format (M3013-500). Order from MBP via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or contact <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a> for volume pricing.

Product Storage

Overexpression Broth™ (OB™) (100 ml)

Overexpression Broth™ (OB™) (500 ml)

PCR Strip MagStand

3DP-1002 by Zymo Research is the PCR Strip MagStand, a magnetic bead separation stand for standard 0.2 mL PCR tubes and strip tubes. Made in the USA; validated for Zymo-Seq RiboFree Total RNA Library Kit (R3000) SPRI bead cleanup and compatible with general AMPure/SPRI workflows in strip-tube format.

<h2>What Are Zymo Research Buffers?</h2>
Zymo Research buffers are formulation-specific reagents used at defined steps &mdash; lysis, binding, wash, and elution &mdash; in Zymo-Spin column-based purification protocols for DNA, RNA, and bisulfite-converted DNA. Each buffer is optimized for a specific kit family: DNA Wash Buffer concentrate (requires ethanol addition before use) removes salts and contaminants before elution from Zymo-Spin silica columns; DNA Elution Buffer (10 mM Tris, pH 8.5, 0.1 mM EDTA) releases purified DNA from the matrix; RNA Wash Buffer supports RNA extraction kits including Quick-RNA and Direct-zol lines; M-Elution Buffer is designed for eluting bisulfite-converted DNA in EZ DNA Methylation kits. Researchers typically order these separately when a single buffer component runs out before the rest of the kit, or when scaling up a protocol beyond the volume included.
TL;DR: Order Zymo buffers individually to replenish a specific step in your existing Zymo workflow without replacing the full kit.
<h2>How to Choose Zymo Research Buffers</h2>
<h3>Match the buffer to your kit family</h3>
Every Zymo buffer is paired to a specific kit line. DNA Wash Buffer and DNA/RNA Wash Buffer concentrate are used in DNA Clean &amp; Concentrator, Quick-DNA, and Zymoclean Gel DNA Recovery kits. RNA Wash Buffer pairs with Quick-RNA kits. ZymoBIOMICS DNA Wash Buffer 1 and Wash Buffer 2 are specific to the ZymoBIOMICS microbiomics line. Substituting a buffer from a different kit family can compromise yield or purity.
<h3>Confirm ethanol addition requirements before use</h3>
Several Zymo wash buffer concentrates require addition of 96-100% ethanol to the bottle before first use &mdash; the label states the exact volume. DNA Wash Buffer concentrate, DNA/RNA Wash Buffer concentrate, and Urine DNA Wash Buffer concentrate all require this step. Pre-made (non-concentrate) versions such as RNA Wash Buffer are supplied ready to use.
<h3>Select the right volume for your throughput</h3>
Zymo buffers are available in sizes from 6 ml (single-experiment replenishment) up to 500 ml (Buffer P1 Red, for high-volume plasmid prep labs). For labs processing more than 50 samples per week, ordering the largest available size reduces per-sample cost and the frequency of reordering.
<h3>Choose elution buffer chemistry based on downstream application</h3>
DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA) is the standard for most PCR and sequencing applications. For applications sensitive to EDTA &mdash; such as some restriction enzyme digests or direct use with divalent cation-dependent enzymes &mdash; eluting in molecular-grade water or a Tris-only buffer is preferable. M-Elution Buffer is specifically formulated for bisulfite-converted DNA and should not be substituted with standard elution buffer in methylation workflows.
<h3>Verify research-use-only status</h3>
All Zymo Research buffers sold through MBP are for research use only (RUO). They are not cleared for in vitro diagnostic use. For clinical or diagnostic laboratory applications, confirm regulatory status with your institutional compliance officer before use.
<h2>Compare Zymo Buffer Types</h2>
<table>
<thead>
<tr>
<th>Buffer Type</th>
<th>Primary Application</th>
<th>Kit Family</th>
<th>Format Available</th>
</tr>
</thead>
<tbody>
<tr>
<td>DNA Wash Buffer (concentrate)</td>
<td>Salt removal before DNA elution</td>
<td>DNA Clean &amp; Concentrator, Quick-DNA</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA/RNA Wash Buffer (concentrate)</td>
<td>Salt removal for dual DNA/RNA preps</td>
<td>Quick-DNA/RNA kits</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA)</td>
<td>DNA release from silica column</td>
<td>All Zymo DNA purification</td>
<td>16 ml &ndash; available sizes vary</td>
</tr>
<tr>
<td>RNA Wash Buffer</td>
<td>RNA purification wash step</td>
<td>Quick-RNA kits</td>
<td>Ready-to-use</td>
</tr>
<tr>
<td>M-Elution Buffer</td>
<td>Elution of bisulfite-converted DNA</td>
<td>EZ DNA Methylation kit families</td>
<td>Small volume</td>
</tr>
<tr>
<td>ZymoBIOMICS DNA Wash Buffer 2</td>
<td>Metagenomic/microbiomic DNA cleanup</td>
<td>ZymoBIOMICS DNA kits</td>
<td>Sold separately</td>
</tr>
<tr>
<td>Buffer P1 Red</td>
<td>Alkaline lysis resuspension (plasmid prep)</td>
<td>Zyppy Plasmid Miniprep</td>
<td>10 ml &ndash; 500 ml</td>
</tr>
</tbody>
</table>
<h2>Specifications Context</h2>
Zymo wash buffer concentrates are supplied at a defined fold-concentration (commonly 4x or 6x) and require ethanol addition to reach working strength &mdash; the exact ratio is printed on the bottle and in the kit protocol. Elution buffer volumes for Zymo-Spin columns typically range from 6 to 100 &micro;l per elution; larger elution volumes improve total yield at the cost of DNA concentration. For DNA elution from Zymo-Spin columns, the protocol recommends a minimum 1-minute contact time between elution buffer and the silica matrix to maximize recovery of fragments 10 kb and above. As of 2025, Zymo Research tests all buffer lots for performance consistency, with integrity guaranteed for up to one year from purchase date.
Browse the full <a href="https://www.notion.so/reagents">Zymo Research reagents collection at MBP</a> for kits and columns that use these buffers, or visit our <a href="https://www.notion.so/reagents/buffers">reagent buffers category</a> for non-Zymo buffer options including ABM and other brands. For volume orders or purchase orders, use the <a href="https://www.notion.so/quickorder">Quick Order</a> tool or <a href="https://www.notion.so/contact-us">contact us</a> directly &mdash; MBP is a registered vendor for institutional procurement systems.
Last updated: June 2025
&nbsp;

Zymo Buffers

Pathogen DNA/RNA Buffer

Pathogen DNA/RNA Buffer by Zymo Research — all-in-one lysis and binding reagent for nucleic acid extraction from viral, bacterial, and protozoan pathogens in vectors and tissue. Available as R1042-1-100 (100 ml) and R1042-1-50 (50 ml).

Pathogen DNA/RNA Buffer (100 ml)

Pathogen DNA/RNA Buffer (50 ml)

Pathogen DNA/RNA Wash Buffer (Concentrate)

Pathogen DNA/RNA Wash Buffer (Concentrate) by Zymo Research is a column wash reagent for Quick-DNA/RNA™ Pathogen Miniprep kits, used to isolate nucleic acids from mosquitoes, ticks, fleas, and mammalian/avian tissue. Available in 6 ml (R1042-2-6) and 48 ml (R1042-2-48) formats for workflow replenishment. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, and many more leading research institutes, serving labs across the United States, Canada, and internationally.

Pathogen DNA/RNA Wash Buffer (Concentrate) (48 ml)

Pathogen DNA/RNA Wash Buffer (Concentrate) (6 ml)

<h2>What are epigenetics NGS library preparation kits?</h2>
&nbsp;
Epigenetics NGS library preparation kits convert the DNA output of an upstream epigenetics workflow into an indexed sequencing library: adapters are ligated to the ends of each DNA fragment, an optional amplification step increases the total amount of library, and indexes are added to allow multiplexed sequencing of many samples in a single run. These kits are specifically optimized for epigenetics-specific inputs, including ChIP-enriched DNA (often low-yield, sheared to 200&ndash;750 bp), chromatin-accessible DNA from ATAC-seq (also low-yield and fragmented), and bisulfite-converted DNA (fragmented and chemically modified, with low-complexity sequence).
&nbsp;
<h2 dir="ltr">What You Will Discover:</h2>
&nbsp;
<ul>
<li dir="ltr">Groundbreaking single-tube processes that integrate bisulfite conversion and library preparation to chart entire genomes with extremely low input needs.</li>
<li dir="ltr">Rapid, efficient transposase-driven methods that seize open chromatin areas and dynamic regulatory environments in only a few hours.</li>
<li dir="ltr">Enzymatic restriction methods combined with targeted sequencing for affordable, high-density CpG coverage without incurring full-genome expenses.</li>
<li dir="ltr">Advanced multi-adapter ligation techniques designed to reduce bias and optimize library retrieval from fragmented or challenging DNA sources.</li>
<li dir="ltr">Post-bisulfite library preparation systems designed explicitly for sub-nanogram DNA quantities, individual cells, or extensively degraded specimens.</li>
<li dir="ltr">Locus-specific, genome-wide enrichment approaches aimed at accurately monitoring and characterizing 5-hydroxymethylcytosine alterations throughout the genome.</li>
</ul>
&nbsp;
<h2>How to choose epigenetics NGS library preparation kits</h2>
&nbsp;
Match your library prep kit to your specific epigenetics input type
ChIP-seq library prep kits are optimized for low-yield sheared chromatin enriched by immunoprecipitation, while ATAC-seq kits work with tagmented open chromatin fragments, and bisulfite or enzymatic methyl-seq library prep kits are specifically formulated for the highly fragmented, low-complexity sequence characteristics of bisulfite-converted DNA input.
Confirm the kit supports your input DNA amount
ChIP experiments, especially those targeting low-abundance transcription factors or run from limited cell numbers, often yield very little enriched DNA, and kits validated for low-input or picogram-scale library preparation are necessary to generate libraries with sufficient complexity without excessive PCR amplification introducing duplicates.
Choose an indexing strategy appropriate to your sample multiplexing plan
Dual-index strategies, using unique combinations of two distinct index sequences per sample, provide more reliable sample demultiplexing in highly multiplexed runs compared with single-index strategies, and confirming whether your kit supports the number of samples and multiplexing depth required is an important selection criterion.
Understand the impact of PCR cycles on library complexity and duplicate rate
Fewer PCR amplification cycles reduce the proportion of duplicate reads in sequencing data, which is especially important for low-complexity epigenetics libraries where overamplification can dominate output with copies of the same original fragment. Efficient ligation chemistry that increases library yield from low input helps minimize required PCR cycles.
Confirm kit compatibility with your downstream analysis pipeline
Some library prep protocols use specific adapter sequences or library structures that require corresponding adjustments in bioinformatics analysis pipelines, and confirming compatibility before sequencing helps avoid downstream analysis failures.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
ChIP-seq library prep kits optimized for low-input or ultra-low-input chromatin are designed to achieve high library complexity from picogram-scale DNA inputs that would otherwise produce low-complexity libraries with standard kits at similar PCR cycle numbers. As of 2026, library prep kits compatible with enzymatic methyl-seq (EM-seq) as well as classical bisulfite-converted DNA continue to expand, enabling researchers to leverage improved DNA quality while maintaining familiar downstream sequencing workflows.
Prepared to enhance your sequencing efficiency? Discover our complete selection of NGS Library Preparation Kits and <a href="https://mbpinc.net/contact-us">contact the MBP team</a> for a quote now.

NGS Libray Preparation Kits

DataSheet

Pico Methyl-Seq™ Library Prep Kit

The Pico Methyl-Seq Library Prep Kit (D5455) by Zymo Research enables whole-genome bisulfite sequencing from as little as 10 pg of input DNA, with a ligation-free, gel-free workflow compatible with FFPE and cfDNA samples for Illumina sequencing.

Sequencing Compatibility

Pico Methyl-Seq™ Library Prep Kit (10 Preps.)

Pico Methyl-Seq™ Library Prep Kit (25 Preps.)

<h2>What Are Genomic DNA Extraction Kits?</h2>
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Genomic DNA extraction kits isolate chromosomal DNA from a wide range of starting materials, including whole blood, cultured cells, solid tissue, bacteria, and yeast, through lysis, binding to a silica membrane or magnetic beads under chaotropic salt conditions, washing, and elution. Standard kits typically yield DNA suitable for PCR, qPCR, restriction enzyme digestion, and routine sequencing applications, while specialized high-molecular-weight (HMW) kits target larger, less fragmented DNA for long-read sequencing workflows.&nbsp;A standard silica spin-column genomic DNA kit covers most PCR, qPCR, genotyping, and routine sequencing applications. Consider a high-molecular-weight DNA extraction kit when your workflow requires minimally fragmented DNA for long-read sequencing or structural variation analysis.
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<h2>What You Will Find</h2>
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<ul>
<li><a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/viral">Viral Genomic DNA</a>: For sensitive monitoring of viral load, pathogen profiling, and molecular surveillance assays using optimized viral formulations.</li>
<li><a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/blood/tissue">Blood/Tissue Genomic DNA</a>: High-molecular-weight genomic DNA extraction from mammalian blood and tissue samples for downstream sequencing applications.</li>
<li><a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/plant">Plant Genomic DNA</a>: High-purity DNA extraction from plant samples with effective removal of common contaminants.</li>
<li><a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/ffpe">FFPE Genomic DNA</a>: Rapid isolation of high-quality genomic DNA from formalin-fixed, paraffin-embedded tissue samples.</li>
<li><a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/urine">Urine Genomic DNA</a>: Purification of cell-free and cellular DNA from urine samples for oncology, biomarker, and clinical research applications.</li>
<li><a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/insect">Insect Genomic DNA</a>: High-yield genomic DNA extraction from entomological samples.</li>
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<h2>How to Choose a Genomic DNA Extraction Kit</h2>
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Starting Material Lysis
Different sample types require different lysis approaches. Blood cells generally lyse readily, while solid tissue often benefits from Proteinase K digestion to ensure complete cellular disruption. Bacteria and yeast may require enzymatic or mechanical cell-wall disruption before standard lysis buffers can effectively release genomic DNA.
Sample Volume and Yield Expectations
DNA yield is closely linked to starting material quantity and quality. Small volumes of fresh whole blood commonly produce several micrograms of genomic DNA using standard silica spin-column workflows, although exact yields vary by kit design, sample condition, and extraction method.
Fragment Size and DNA Integrity
Standard genomic DNA extraction kits are optimized for PCR, qPCR, and routine sequencing applications and typically recover DNA fragments under 30-50 kb. High-molecular-weight extraction kits use gentler lysis and purification chemistries to preserve longer DNA fragments, often exceeding 50 kb, making them well-suited to long-read sequencing technologies.
RNA Removal and Purity
Many genomic DNA extraction kits incorporate RNase A treatment to remove RNA contamination. This helps improve nucleic acid purity and can be particularly important for downstream applications that are sensitive to RNA carryover, including certain sequencing and library preparation workflows.
Sample Age and Storage History
DNA yield and integrity may be influenced by sample storage conditions and age. Fresh samples generally provide the highest-quality DNA, while archived or shipped specimens may exhibit some degree of DNA degradation or reduced yield that should be considered during experimental planning.
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<h2>Specifications Context</h2>
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Standard genomic DNA extraction kits rely on silica spin-column or magnetic bead-based purification workflows. Following cell or tissue lysis, genomic DNA binds to the purification matrix under chaotropic salt conditions while proteins, inhibitors, and other contaminants are removed during wash steps before final elution in water or low-salt buffer.&nbsp;These workflows generate DNA suitable for PCR, qPCR, restriction digestion, genotyping, and routine sequencing applications. RNase A treatment is commonly included to minimize residual RNA contamination and improve overall nucleic acid purity.&nbsp;High-molecular-weight genomic DNA extraction kits use gentler lysis and purification methods, including specialized magnetic bead or glass bead-based workflows, to minimize DNA shearing. These approaches can preserve DNA fragments exceeding 50-70 kb and are commonly selected for long-read sequencing platforms where fragment length directly influences read length and assembly quality.&nbsp;Genomic DNA extraction kit buffers are typically stable at room temperature, while enzyme components may require refrigeration or freezing. Lot-specific Certificates of Analysis (CoAs) are available on request.
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Genomic DNA

Pinpoint™ Slide DNA Isolation System

C1011-ZYM by Zymo Research — capped silica spin columns for purification of DNA (up to 25 µg) and RNA (up to 50 µg) in ≥ 25 µl eluate. 900 µl volumetric capacity; compatible with microcentrifuge and vacuum manifold. Available in 50-column and 250-column packs. Contact MBP for pricing and availability.