Nest Scientific Consumables

Cryo Boxes

<h2>What Are Cryo Boxes?</h2>
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Cryo boxes are lidded, gridded storage containers designed to organize cryogenic vials, microcentrifuge tubes, and centrifuge tubes inside ultra-low temperature (ULT) freezers at &minus;80&deg;C, vapor-phase liquid nitrogen refrigerators (&minus;150 to &minus;190&deg;C), and cryogenic dewars. The two primary materials are water-resistant coated cardboard (economical, single-use) and rigid polycarbonate or polypropylene (reusable, autoclavable, transparent). Standard well configurations&mdash;25 (5&times;5), 81 (9&times;9), and 100 (10&times;10)&mdash;in 2-inch and 3-inch box heights match common cryogenic vial sizes. The alphanumeric grid on the lid helps with sample tracking in LIMS systems. Cardboard cryo boxes are vapor-phase LN2 rated only; polycarbonate is required for autoclave use or liquid-phase LN2 immersion.
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<h2>What you will find:</h2>
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&bull; Standard cryo boxes for cryogenic vial storage &bull; Freezer storage boxes for ultra low temperature use &bull; Cardboard cryo boxes for cost effective storage &bull; Polypropylene cryo boxes for durability and reuse &bull; Grid format boxes for easy sample organization &bull; Hinged lid cryo boxes for quick access &bull; Color coded storage boxes for sample identification &bull; Cryo storage boxes for 1.5 mL and 2.0 mL vials
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<h2>How to Choose Cryo Boxes</h2>
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Material: Cardboard vs. Polycarbonate vs. Polypropylene
Cardboard cryo boxes are economical and used for long-term storage at &minus;80&deg;C or vapor-phase LN2. They are single-use and not autoclavable. Polycarbonate (PC) boxes are durable, transparent, and autoclavable at 121&deg;C, making them suitable for frequent handling. Polypropylene (PP) boxes also tolerate autoclaving and offer higher chemical resistance than PC. Choice depends on reuse frequency and sterilization needs.
Well Count and Vial Size
81-well (9&times;9) boxes fit standard 1.8&ndash;2.0 mL cryogenic vials up to ~13 mm outer diameter. 100-well (10&times;10) boxes are used for smaller 1.2&ndash;1.5 mL vials and microcentrifuge tubes up to ~12 mm OD. Always match vial diameter with box well size before ordering.
Box Height: 2-Inch vs. 3-Inch
2-inch boxes are standard for 1&ndash;2 mL cryovials and are most commonly used in biobanking. 3-inch boxes are for larger 3&ndash;5 mL vials and certain centrifuge tubes where extra depth is required.
LN2 Phase Compatibility
Both cardboard and hard-wall boxes can be used in vapor-phase LN2. Only polycarbonate or polypropylene boxes should be used for liquid-phase LN2 because cardboard can break down when submerged.
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<h2>Specifications Context</h2>
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Cryo boxes are typically rated from &minus;196&deg;C to +121&deg;C depending on material. Cardboard is suitable for vapor-phase LN2 only, while hard-wall plastic boxes can handle more demanding workflows. Proper selection depends on vial size, storage temperature, and how frequently samples are accessed.
Eliminate the risk of thermal damage. <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">Connect with the experts at Molecular Biology Products Inc.</a> to find the perfect storage solution for your high-value specimens.

Datsheet

Cryo Box for Brooks, 8*6, 1/pk, 10/cs

Nest Scientific Cryo Box for Brooks automated biorepository systems — 8×6, 48-position, 1/pk, 10/cs. Two variants: 616931 (for 1.9 mL SBS cryovials 612041/047/051) and 616932 (for 4.0 mL SBS cryovials 614041/047/051).

Manufacturer

Cryo Box for Brooks 1.9ml Cryovials 2D barcode on bottom 1D barcode on side (item # 612041 612047 and 612051), 8*6, 1/pk, 10/cs

Cryo Box for Brooks 4.0ml Cryovials 2D barcode on bottom 1D barcode on side (item # 614041 614047 and 614051), 8*6, 1/pk, 10/cs

Round Well Deep Well Plates

<h2 data-section-id="8npnrj" data-start="0" data-end="40">What are Round Well Deep Well Plates?</h2>
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Round well deep well plates are polypropylene microplates manufactured to ANSI/SLAS (SBS) footprint standards, featuring cylindrical wells that provide greater sample volumes than standard PCR or assay plates. Typical formats include 96-well plates with capacities of 1.2 mL, 2.0 mL, or 2.2 mL per well, designed for automated sample processing, nucleic acid extraction, microbial culture, compound storage, and high-throughput laboratory workflows.
The round well geometry promotes uniform mixing, consistent liquid movement during shaking, and efficient magnetic bead collection during automated extraction procedures. Unlike square well plates, which maximize storage volume, round well plates are preferred when sample recovery, mixing performance, and magnetic bead handling are critical.
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<h2 dir="ltr">What you will find:</h2>
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96-round well storage plates
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1.2ml deep well plates
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2.0ml round well plates
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Sterile deep well microplates
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DNase and RNase-free plates
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U-bottom deep well plates
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Polypropylene storage plates
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Automation-compatible round well plates
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Silicone sealing mats
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<h2 data-section-id="m2xmka" data-start="1392" data-end="1436">How to Choose Round Well Deep Well Plates</h2>
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Well Volume
The 1.2 mL round well plate is specified for smaller-input nucleic acid extraction protocols (blood, plasma, swab) and fits automated deck formats with restricted Z-height clearance. The 2.0 mL round well format accommodates larger sample input volumes for high-yield extractions and is the standard format for Nest Scientific deep well plates used in nucleic acid processing. Confirm working volume versus total well volume in the product specification &mdash; a 2.0 mL plate typically has a total volume of 2,200&ndash;2,400 &micro;L.
Bottom Geometry
U-bottom (hemispherical) round wells are standard for sample storage, bacterial and cell culture, and general liquid handling where complete aspiration is not required. V-bottom (conical) round wells concentrate liquid and pellet to the lowest point for complete aspiration and enhanced pellet recovery, preferred in enzymatic assay and precipitation protocols. Flat-bottom round well plates are used when an optical flat surface is needed for absorbance-based assay measurement.
Sterility Requirements
Non-sterile round well deep well plates manufactured in ISO 8 cleanrooms satisfy most nucleic acid extraction and sample processing workflows. Sterile options (gamma-irradiated, SAL 10⁻⁶) are required for cell culture, bacterial culture, and RNA extraction from live cells. Autoclavable polypropylene formats provide a cost-effective reusable sterile option for labs with autoclave access.
Centrifugation and Sealing
Standard round well deep well plates are rated 4,000&ndash;6,000 &times; g. Raised well rims ensure consistent sealing with adhesive films, heat-seal foils, and pre-slit silicone mats. Nest Scientific 96-well round well silicone sealing mats (pre-slit, 7 mm diameter) are compatible with 0.35&ndash;1.35 mL round well plates.
Automation Compatibility
Specify virgin polypropylene, lot-certified DNase-free, RNase-free, DNA-free, and PCR inhibitor-free for nucleic acid extraction. Nest Scientific deep well plates use USP Class VI polypropylene material with high chemical stability for demanding extraction workflows.
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<h2 data-section-id="1jl751w" data-start="4818" data-end="4843">Specifications Context</h2>
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Round well deep well plates are manufactured from high-purity polypropylene and are commonly rated for temperatures from &minus;80&deg;C to +121&deg;C, supporting freezer storage and autoclave sterilization. Premium plates are produced from USP Class VI resins and tested for DNase, RNase, DNA, and PCR inhibitor contamination.
The 96-well 2.0 mL U-bottom round well plate remains one of the most widely used formats for automated nucleic acid extraction, biobanking, bacterial culture, and sample preparation workflows due to its balance of volume capacity, automation compatibility, and reliable magnetic bead performance.
Browse our range and <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">request a quote from the MBP team</a> to find the right fit for your lab.

Deep Well Plate (Round well)

Nest Scientific deep well plates, round well, PP — U-bottom and V-bottom in 48-well and 96-well formats, 0.36–3.5 mL/well. Non-sterile and sterile versions. 14 SKUs: 504102/162 (3.5mL 48W), 501102/162 (0.4mL 96W), 502102/162 (1.0mL 96W U), 503102/162 (2.0mL 96W), 500101/161 (0.36mL V), 501101/601 (0.5mL V), 502101/601 (1.0mL V LP), 507001/101 (1.3mL). 5–10/pk, 50/cs.

3.5 ml 48-Well Deep Well Plate, U-Bottom, Round well, non-sterile, 5/pk, 50/cs

0.4 ml 96-Well Deep Well Plate, U-Bottom, Round well, non-sterile, 10/pk, 50/cs

1.0 ml 96-Well Deep Well Plate, U-Bottom, Round well, non-sterile, 5/pk, 50/cs

2.0 ml 96-Well Deep Well Plate, U-Bottom, Round Well, non-sterile, 5/pk, 50/cs

0.36 ml 96-Well Deep Well Plate, V-Bottom, Round well, non-sterile, 5/pk, 50/cs

0.5ml 96-Well Deep Well Plate, V-Bottom, Round well, non-sterile, 10/pk, 50/cs

1.0 ml 96-Well Deep Well Plate, V-Bottom, low profile, Round well, non-sterile, 5/pk, 50/cs

3.5 ml 48-Well Deep Well Plate, U-bottom, Round well, Sterile, 5/pk, 50/cs

0.4 ml 96-Well Deep Well Plate, U-Bottom, Round well, sterile, 10/pk, 50/cs

1.0 ml 96-Well Deep Well Plate, U-Bottom, Round well, Sterile, 5/pk, 50/cs

2.0 ml 96-Well Deep Well Plate, U-Bottom, Round Well, Sterile, 5/pk, 50/cs

0.36 ml 96-Well Deep Well Plate, V-Bottom, Round well, sterile, 5/pk, 50/cs

0.5 ml 96-Well Deep Well Plate, V-Bottom, Round well, sterile, 10/pk, 50/cs

1.0 ml 96-Well Deep Well Plate, V-Bottom, low profile, Round well, sterile, 5/pk, 50/cs

1.3 mL 96-Well Deep Well Plate, U-Bottom, Round Well, Non-Sterile, 5/pk, 50/cs

1.3 mL 96-Well Deep Well Plate, U-Bottom, Round Well, Sterile, 5/pk, 50/cs

Square Well Deep Well Plates

<h2 data-section-id="7nn2n" data-start="0" data-end="42">What Are Square Well Deep Well Plates?</h2>
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Square well deep well plates are polypropylene microplates designed with square-shaped wells instead of traditional round wells. This design maximizes liquid capacity within the standard SBS/ANSI microplate footprint, making them ideal for high-throughput laboratory workflows that require larger sample volumes. Commonly available in 96-well formats with capacities ranging from 1.1 mL to 2.2 mL per well, these plates are widely used for nucleic acid extraction, protein precipitation, compound library storage, sample preparation, and automated liquid handling.
The square-well geometry increases usable volume while maintaining compatibility with automated platforms from Hamilton, Tecan, Beckman Coulter, and Agilent. Manufactured from virgin polypropylene, square well plates offer excellent chemical resistance to DMSO, ethanol, acetonitrile, and extraction buffers, while minimizing sample binding and contamination risks.
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<h2 dir="ltr">Product Range:</h2>
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96-square well storage plates for sample management.
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2.2ml deep well plates for large-volume handling.
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V-bottom square well plates for maximum sample recovery.
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DNA-free deep well microplates for molecular applications.
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Medical-grade polypropylene plates for chemical durability.
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Automation-ready square well formats for robotic integration.
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Deep well plates for bead beating and high-impact use.
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Chemical-resistant 96-well plates for harsh reagents.
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<h2 data-section-id="1lthnju" data-start="1133" data-end="1179">How to Choose Square Well Deep Well Plates</h2>
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Well Volume The most common formats are 2.0 mL and 2.2 mL square well plates, which provide sufficient volume for nucleic acid extraction, protein precipitation, and compound storage workflows. Lower-profile 1.1 mL plates are useful when deck height limitations or freezer space optimization are important considerations.
Bottom Geometry V-bottom wells concentrate liquid and pellets into a single point, enabling more complete aspiration and improved recovery during centrifugation-based workflows. U-bottom wells promote better mixing and resuspension, making them suitable for microbial culture applications and serial dilutions.
Centrifugation Requirements Most square well polypropylene plates are compatible with centrifugation speeds between 4,000 &times; g and 6,000 &times; g. For demanding extraction protocols, reinforced plate designs provide additional structural stability during high-speed centrifugation.
Automation Compatibility Select plates that comply with ANSI/SLAS standards and include molded alphanumeric indexing for sample tracking. Features such as stacking lugs and raised well rims improve robotic handling and sealing performance.
Certification Standards For molecular biology applications, choose plates manufactured from virgin polypropylene and certified DNase-free, RNase-free, DNA-free, and PCR inhibitor-free. Sterile options are recommended for sensitive workflows and regulated environments.
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<h2 data-section-id="u7fdje" data-start="3248" data-end="3283">Specifications and Applications</h2>
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Square well deep well plates are typically manufactured from high-purity polypropylene and operate across a wide temperature range, often from approximately &ndash;86&deg;C to +125&deg;C. Most plates conform to ANSI/SLAS microplate dimensions (127.76 &times; 85.48 mm), ensuring compatibility with automated liquid handlers, centrifuges, incubators, and storage systems.
Today, 2.0 mL and 2.2 mL square well V-bottom plates remain the preferred format in pharmaceutical research, clinical diagnostics, genomics laboratories, and biobanking facilities due to their high volume capacity, efficient liquid recovery, and seamless integration into automated workflows.
Upgrade your lab with durable essentials that won't fail during critical runs. Browse our website and <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">request a quote from the team at MBP</a> for your supplies.

Deep Well Plate (Square well)

NEST SBS-compliant square-well deep well plates in 96-, 48-, 24-, and 384-well formats. Volumes from 120 µL to 16 mL; U-bottom, V-bottom, I-shaped (Kingfisher); sterile and non-sterile. SKUs 501002–504763. 5/pk, 50/cs.

1.0 ml 96-Well Deep Well Plate, U-Bottom, Square Well, non-sterile, 5/pk, 50/cs

2.2 ml 96-Well Deep Well Plate, V-Bottom, Square well, non-sterile, 5/pk, 50/cs

2.2 ml 96-Well Deep Well Plate, U-Bottom, Square Well, non-sterile, 5/pk, 50/cs

2.2 mL 96-Well Deep Well Plate, I-Shaped, U-Bottom, Square Well, Non-Sterile, 5/pk, 50/cs

2.2 mL 96-Well Deep Well Plate, I-Shaped, U-Bottom, Square Well, Sterile, 5/pk, 50/cs

4.6 ml 48-Well Deep Well Plate, U-Bottom, Square well, non-sterile, 5/pk, 50/cs

1.6 ml 96-Well Deep Well Plate, U-Bottom, Square Well, non-sterile, 5/pk, 50/cs

4.6 ml 48-Well Deep Well Plate, U-bottom, Square well, Sterile, 5/pk, 50/cs

1.0 ml 96-Well Deep Well Plate, U-Bottom, Square Well, Sterile, 5/pk, 50/cs

1.6 ml 96-Well Deep Well Plate, U-Bottom, Square Well, Sterile, 5/pk, 50/cs

2.2 ml 96-Well Deep Well Plate, U-Bottom, Square Well, Sterile, 5/pk, 50/cs

2.2 ml 96-Well Deep Well Plate, V-Bottom, Square well, sterile, 5/pk, 50/cs

16 mL 24-Well Deep Well Plates, V Bottom, Square Well, Sterile, 1/pk, 25/cs

120 ul 384 well Deep Well Plates, V Bottom, Square Well, Non-Sterile, 5/pk, 50/cs

120 ul 384 well Deep Well Plates, V Bottom, Square Well, Sterile, 5/pk, 50/cs

240 ul 384 well Deep Well Plates, V Bottom, Square Well, Non-Sterile, 5/pk, 50/cs

240 ul 384 well Deep Well Plates, V Bottom, Square Well, Sterile, 5/pk, 50/cs

Shoe Covers

<h2 data-section-id="jov9my" data-start="0" data-end="35">What Are Laboratory Shoe Covers?</h2>
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Laboratory shoe covers are disposable protective overshoes worn over regular footwear before entering laboratories, cleanrooms, pharmaceutical facilities, healthcare environments, and other controlled spaces. Their primary purpose is to prevent dirt, dust, microorganisms, and contaminants from being transferred into sensitive work areas through shoe soles while also protecting personnel footwear from exposure to chemicals, biological materials, and floor contaminants.
Most laboratory shoe covers are manufactured from non-woven polypropylene (PP), which provides lightweight particulate protection and low lint generation. Specialized options such as SMS, PE-coated polypropylene, CPE, and Tyvek&reg; offer additional protection against liquids, aerosols, and chemical splashes for more demanding applications.
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<h2 dir="ltr">What you will find:</h2>
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Disposable polypropylene shoe covers for general lab use
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Non-skid shoe covers for improved floor safety
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Elastic-edge shoe covers for secure universal fit
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Cleanroom-compatible shoe covers for controlled environments
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Water-resistant shoe covers for added protection
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Lightweight breathable shoe covers for long wear comfort
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Anti-slip sole shoe covers for stability in lab areas
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Universal-size shoe covers for easy bulk use
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Low-lint contamination-control shoe covers for sterile workflows
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<h2 data-section-id="1czudfj" data-start="1053" data-end="1092">How to Choose Laboratory Shoe Covers</h2>
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Material Spunbond polypropylene (28&ndash;40 gsm) is lightweight, breathable, and provides general particulate barrier for most research and pharmaceutical lab environments. PE-coated PP or CPE (chlorinated polyethylene) provides waterproof liquid resistance for wet benches, chemical spill environments, and animal facilities. SMS fabric (spunbond-meltblown-spunbond) adds aerosol and droplet barrier performance for pharmaceutical cleanrooms and clinical diagnostic labs. Tyvek shoe covers provide the highest durability and chemical splash protection for hazardous material handling. &nbsp; Non-skid sole Non-skid shoe covers feature anti-skid latex tread sprayed to the bottom sole, providing traction on smooth lab floor surfaces &mdash; polished concrete, vinyl tile, and epoxy-coated floors common in cleanrooms and pharmaceutical facilities. Non-skid covers are standard for any environment where wet floor conditions or smooth surfaces create slip risk. Plain covers are appropriate for brief-wear or dry rough-floor environments only. &nbsp; ISO cleanroom rating General-purpose PP shoe covers manufactured in ISO-accredited facilities with lot-number traceability are rated for ISO 7 (Class 10,000) environments. Critical-environment cleanroom covers with lint-free laminated fabric, bonded seams, and documented particle counts are available for ISO 5&ndash;6 (Class 100&ndash;1,000) environments. Confirm the product's documented ISO rating when sourcing for GMP-regulated or validated cleanroom environments. &nbsp; Case quantity and floor stock Standard PP shoe cover cases hold 300 pieces (150 pairs) or 600 pieces (300 pairs). Match case size to daily consumption: a 10-person lab with two cover changes per day uses approximately 20 pairs/day, or one 300-piece case per 15 working days. Bulk case ordering through MBP's Quick Order tool simplifies floor stock management and PO-based replenishment. &nbsp; Latex-free elastic cuffs Quality-tier shoe covers use latex-free elastic ankle cuffs &mdash; confirm latex-free elastic specification for environments with latex allergy protocols. All PP, CPE, and SMS upper materials are inherently latex-free.
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<h2 data-section-id="1jl751w" data-start="4537" data-end="4562">Specifications Context</h2>
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Laboratory shoe covers are commonly manufactured from low-lint, non-woven materials that support contamination-control programs in research, pharmaceutical, biotechnology, and healthcare facilities. Many cleanroom-grade products are produced in ISO-certified manufacturing environments with lot traceability for quality assurance and regulatory compliance.
Step into cleaner workflows&mdash;choose MBP shoe covers that keep every entry controlled and every surface protected.&nbsp; <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact us for a quick quote</a>.

Disposable PP non-woven shoe covers, 100/pk, 500/cs

923001 by MBP Inc. are disposable polypropylene non-woven shoe covers for cleanrooms, pharmaceutical facilities, hospitals, and electronics manufacturing. PP non-woven fabric provides a low-lint particle barrier to prevent shoe-borne contamination. Supplied 100/pk, 500/cs.

Erlenmeyer Flasks

<h2 dir="ltr">What are Cell Culture Erlenmeyer Flasks?</h2>
 Cell culture Erlenmeyer flasks (also called shake flasks or conical flasks) are single-use, sterile plastic vessels with a conical body, flat base, and narrow neck used for suspension cell culture on orbital shakers and incubator shakers, microbial culture, and media preparation. Unlike rectangular T-flasks designed for static monolayer culture, Erlenmeyer flasks are engineered for shaking: their wide conical base maximizes liquid surface area for oxygen transfer, while the narrow neck minimizes evaporation and contamination risk. Choose PC Erlenmeyer flasks for autoclavable reuse and high-temperature media preparation; choose PETG for single-use suspension culture and processes requiring BPA-free materials.
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<h2 dir="ltr">What you will find:</h2>
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<a href="erlenmeyer-flasks/petg-erlenmeyer-flasks">PETG Erlenmeyer flasks: </a>strong choice for cell culture applications that need excellent clarity, reliable handling, and convenient single-use performance. They are lightweight, durable, and ideal for shaker culture, media preparation, and general suspension cell workflows.
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<a href="erlenmeyer-flasks/pc-erlenmeyer-flasks">PC Erlenmeyer flasks: </a>built for applications that require greater strength and higher impact resistance. They are well-suited for demanding lab environments, including vigorous shaking, transport, and workflows where added durability is important.
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<li dir="ltr" aria-level="1"><a href="https://mbpinc.net/consumables/cell-culture/erlenmeyer-flasks/erlenmeyer-flask-accessories">Erlenmeyer Flask Accessories: </a>Includes bi-directional, inverted, and multifunctional transfer caps, plus silicon tube assemblies for 2 L, 3 L, 3 L wide-mouth, and 5 L high-efficiency flasks, supporting sterile transfer and closed-system workflows.</li>
</ul>
<h2 dir="ltr"> How to Choose Erlenmeyer Flasks for Cell Culture</h2>
 PC vs. PETG Material PC (polycarbonate) Erlenmeyer flasks are autoclavable at 121 &deg;C (typically 5&ndash;10 cycles), shatter-proof, crystal-clear, and rated from &minus;135 &deg;C to +135 &deg;C. PC is ideal for labs that sterilize media or wash in-house. PC contains BPA. PETG Erlenmeyer flasks are single-use, BPA-free, rated from &minus;80 &deg;C to +60 &deg;C, and cannot be autoclaved. They provide a contamination-free, cleaning-free alternative to glass and PC for high-throughput suspension culture operations. Flat Base vs. Baffled Base Flat-base Erlenmeyer flasks provide gentler swirling for slow-growth cell lines and media mixing. Baffled-base flasks have three or four angled internal baffles that interrupt the swirling flow, increasing turbulence, raising dissolved oxygen (DO) by up to 2&times; compared to flat-base flasks at the same orbital speed, and reducing cell sedimentation. Baffled flasks are the standard for CHO-S, HEK293-F, Sf9, microbial, and yeast cultures requiring high oxygen transfer rates. NEST Scientific offers baffled and flat-base versions in both PC and PETG across all core volumes. Vented vs. Plug Seal Cap Vented caps contain a 0.22 &micro;m hydrophobic PTFE membrane that allows free O₂/CO₂ gas exchange while excluding microbial contamination. The NEST vented cap features an oversized vent area that improves gas exchange beyond standard-size membrane caps, supporting oxygen-demanding suspension cultures at higher cell densities. Plug seal caps create a fully sealed environment for media storage, static incubation, or transport outside the incubator. Always use vented caps for actively growing suspension cultures on an orbital shaker. Volume and Fill Ratio Erlenmeyer flasks should be filled to no more than 20&ndash;25% of the nominal flask volume for suspension shaker culture to maintain adequate headspace for oxygen transfer. A 500 mL flask running at 20% fill holds 100 mL of culture; a 1,000 mL flask holds 200 mL. Overfilling reduces oxygen transfer efficiency and creates foam that blocks the vented cap membrane. For media storage (no shaking), the fill volume can approach 80% of nominal. Shaker Compatibility NEST Scientific Erlenmeyer flasks (125 mL&ndash;3,000 mL) are compatible with standard laboratory orbital shakers, including those from Infors HT, New Brunswick Scientific (Innova), and Thermo Scientific MaxQ. Confirm flask neck O.D. and base diameter against your shaker's flask holder clips or clamp system before ordering large quantities. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific PC Erlenmeyer flasks are manufactured from USP Class VI medical-grade polycarbonate, gamma sterilized per ISO 11137 to SAL 10⁻⁶, and individually wrapped in sterile packaging. PETG Erlenmeyer flasks are E-beam sterilized and certified DNase/RNase-free, pyrogen-free per lot. Vented cap PTFE membranes are 0.22 &micro;m with an oversized vent area that increases gas exchange efficiency vs. standard-diameter membrane caps. NEST Scientific is ISO 9001:2015 and ISO 13485 certified with FDA registration. Flask catalog sizes extend to high-efficiency 2 L and 5 L PC formats (NEST High Efficiency Erlenmeyer Flask line) for production-scale suspension culture. MBP stocks the NEST Scientific Erlenmeyer flask line across all major volumes and configurations. 
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Support accurate mixing and culturing with premium Erlenmeyer flasks designed for modern laboratories. <a href="https://www.mbpinc.net/contact-us" title="Contact the MBP Team" style="color: #236fa1; text-decoration: underline;">Contact the MBP team</a>&nbsp;for a formal quote.

Product Literature

Flyer

Erlenmeyer Flask with Bi-Directional Transfer Cap TPE Tube, Heat seal, Vent Filter

NEST Erlenmeyer Flask with Bi-directional Transfer Cap: 250 mL Erlenmeyer flask with bi-directional transfer cap, 50 cm Weldable TPE tube (1/8&quot; ID &times; 1/4&quot; OD), PTFE internal tube, 0.22 &mu;m PTFE vent filter (13.8 cm&sup2;), factory heat-sealed, E-beam sterile SAL 10&minus;6, ISO 13485 facility, DNase/RNase/pyrogen-free. 1/pk, 4/cs. Available in 250mL (C30921-AZC050A), 1000mL (C31121-AZC050A), or 2L high efficiency (C10122-AZB050A).

2L High Efficiency Erlenmeyer Flask with Bi-directional Transfer Cap with TPE Tube (50cm 1/8" ID 1/4" OD), Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 4/cs

250mL Erlenmeyer Flask with Bi-Directional Transfer Cap, TPE Tube (50cm 1/8" ID 1/4" OD) , Heat seal ,Vent Filter (0.22 μm 13.8cm²), Sterile, 1/pk, 4/cs

1000 mL Erlenmeyer Flask with Bi-Directional Transfer Cap, TPE Tube (50cm1/8" ID 1/4" OD), Heat seal , Vent Filter (0.22 μm 13.8cm2), Sterile, 1/pk, 4/cs

Externally Threaded Cryogenic Vials

<h2 data-section-id="17lohz5" data-start="0" data-end="48">What Are Externally Threaded Cryogenic Vials?</h2>
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Externally threaded cryogenic vials have the screw thread on the outside of the vial neck, with a cap that covers the entire top portion of the vial when closed. Manufactured from cryogenic-grade virgin polypropylene, these vials are designed to withstand temperatures from &minus;196&deg;C to +121&deg;C and are commonly used for long-term storage of cell lines, serum, blood, DNA, RNA, bacterial cultures, and other biological samples. Available in volumes ranging from 1.2 mL to 5 mL, they are offered in both self-standing and round-bottom formats. Unlike internally threaded vials, external-thread designs use an integrated sealing system rather than a separate O-ring, helping reduce handling issues. For automated decapping systems, high-throughput biobanking, and SBS-format robotic workflows, externally threaded vials are the preferred format.
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<h2 dir="ltr">MBP Offers:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
2D Barcoded Cryogenic Vials for automated tracking
</li>
<li dir="ltr" aria-level="1">
SBS Format Cryo Racks for robotic integration
</li>
<li dir="ltr" aria-level="1">
3D Barcoded Storage Vials for digital inventory
</li>
<li dir="ltr" aria-level="1">
Color-coded Cap Inserts for rapid identification
</li>
<li dir="ltr" aria-level="1">
Self-standing Star-foot Cryo Vials
</li>
<li dir="ltr" aria-level="1">
Silicone O-ring Sealed Leak-proof Containers
</li>
<li dir="ltr" aria-level="1">
Gamma-sterilized Aseptic Storage Tubes
</li>
</ul>
&nbsp;
<h2 data-section-id="px7lzo" data-start="891" data-end="943">How to Choose Externally Threaded Cryogenic Vials</h2>
&nbsp;
Volume
The 2 mL externally threaded vial is the industry standard for cell banking, serum aliquots, and general biospecimen storage. It fits standard 81-well cryo boxes and is suitable for most laboratory applications. Smaller 1.2&ndash;1.5 mL vials are ideal for high-density storage where space efficiency is important, while larger 3&ndash;5 mL formats are commonly used for tissue samples, bacterial glycerol stocks, and larger-volume specimens.
Base Style: Self-Standing vs. Round Bottom
Self-standing vials feature a star-foot or conical base that allows them to stand upright on laboratory benches without requiring a rack, reducing the risk of spills during handling. Round-bottom vials require rack support but often offer broader compatibility with different storage racks and cryo box configurations.
Barcoding: Plain vs. 2D Barcoded
Standard externally threaded vials include a writable surface for manual labeling and are suitable for small to medium-sized sample collections. For large biobanks and automated sample management systems, 2D barcoded vials provide Data Matrix codes that can be scanned directly into Laboratory Information Management Systems (LIMS), improving traceability and reducing manual data entry errors.
Sterility and Purity
Cryogenic vials intended for cell culture, molecular biology, and clinical research should be gamma sterilized and certified RNase-free, DNase-free, non-pyrogenic, and endotoxin-free. These certifications help protect sample integrity and reduce contamination risks during long-term storage.
&nbsp;
<h2 data-section-id="1jl751w" data-start="3061" data-end="3086">Specifications Context</h2>
&nbsp;
Externally threaded polypropylene cryogenic vials are designed for vapor-phase liquid nitrogen storage and ultra-low temperature freezers. The standard outer diameter of a 2 mL vial is approximately 12.5 mm, making it compatible with most 81-well cryo box formats. Modern external-thread designs use integrated thermoplastic elastomer sealing systems that eliminate the need for separate O-rings, reducing the possibility of seal misalignment or loss during routine handling. As of 2026, externally threaded 2 mL vials with SBS-compatible footprints and 2D barcodes are widely used in large-scale biobanking and sample repository applications.
Your research is priceless. Your storage should be equally reliable. <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">Connect with Molecular Biology Products Inc.</a> for a personalized quote.

External Thread Self-Standing Cryogenic Vial, Sterile, New Model, Box

The External Thread Self-Standing Cryogenic Vials by NEST Scientific are sterile, self-standing external-thread PP cryovials rated to −196°C in liquid nitrogen vapor phase. Certified DNase-free, RNase-free, human DNA-free, and non-pyrogenic; manufactured under cGMP and ISO 13485. Available in 1.0 mL (618901), 1.5 mL (606901), 2.0 mL (607401), 4.0 mL (608401), and 5.0 mL (609401).

1.5 mL Cryogenic Vial, Self-Standing, External Thread, Sterile, New Model, 50/pk, 500/box, 2000/cs

2.0 mL Cryogenic Vial, Self-Standing, External Thread, Sterile, New Model, 50/pk, 500/box, 2000/cs

4.0 mL Cryogenic Vial, Self-Standing, External Thread, Sterile, New Model, 50/pk, 250/box, 1000/cs

5.0 mL Cryogenic Vial, Self-Standing, External Thread, Sterile, New Model, 50/pk, 250/box, 1000/cs

1.0 mL Cryogenic Vial, Self-Standing, External Thread, Sterile, New Model, 96/pk, 960/cs

External Thread Self-Standing Cryogenic Vial, Sterile, New Model, Racked

External Thread Self-Standing Cryogenic Vial, Sterile, New Model, Racked by NEST Scientific are USP Class VI PP cryogenic vials rated to −196 °C (vapor-phase LN₂) and autoclavable to 121 °C. E-beam sterilized, DNase/RNase-free, human DNA-free, non-pyrogenic. Available in 1.0mL (618906), 1.5mL (606902), 2.0mL (607402), 4.0mL (608402), and 5.0mL (609402), racked.

1.5 mL Cryogenic Vial, Self-Standing, External Thread, Sterile, New Model, 10*10/rack, 14 racks/cs, 1400 vials/cs

2.0 mL Cryogenic Vial, Self-Standing, External Thread, Sterile, New Model, 10*10/rack, 12 racks/cs, 1200 vials/cs

4.0 mL Cryogenic Vial, Self-Standing, External Thread, Sterile, New Model, 10*10/rack, 8 racks/cs, 800 vials/cs

5.0 mL Cryogenic Vial, Self-Standing, External Thread, Sterile, New Model, 10*10/rack, 6 racks/cs, 600 vials/cs

1.0 mL Cryogenic Vial, Self-Standing, External Thread, Sterile, New Model, 12*8/rack, 10 racks/cs, 960 vials/cs

BioFactory Accessories

<h2 dir="ltr"> What are Accessories for NEST BioFactory&trade; Systems?</h2>
 NEST BioFactory&trade; accessories are the replacement caps, silicone tubing, vent filters, overcaps, and pre-assembled closed-system cap assemblies used to configure BioFactory units for closed aseptic liquid transfer, gas exchange management, and multi-unit serial connections in cGMP and research-scale bioprocess workflows. The accessory set enables the BioFactory narrow-mouth cap to function as a closed-system liquid transfer port &mdash; media, wash buffer, trypsin, and cell harvest supernatant are transferred in and out through the silicone tubing connection without opening the vessel. Accessories for the BioFactory are required when operating the BioFactory as a closed system; for simple open-pour workflows using wide-mouth caps, standard vent and plug seal caps are the only accessories needed.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="color: #000000;" aria-level="1">
BioFactory media transfer caps
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Sterile tubing and connector sets
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Fluid transfer adapters for cell culture vessels
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
BioFactory bottle caps and closures
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Sterile quick-connect systems
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Liquid handling accessories
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Vessel handling and support tools
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Closed-system connectors for aseptic processing
</li>
</ul>
<h2 dir="ltr"> Key BioFactory Accessory Categories</h2>
 BioFactory Cap with Silicone Tube (CPC or Luer Connector) The BioFactory cap with silicone tube is the primary closed-transfer accessory: an adaptor cap that fits the narrow-mouth port of the BioFactory unit, pre-assembled with 80 cm of silicone tubing and a terminal connector &mdash; either a male CPC (clamped pressure connector / quick-connect) or a standard Luer lock &mdash; for coupling to peristaltic pumps, other BioFactory units, centrifuge tubes, or PETG media bottle assemblies. Two tubing I.D. &times; O.D. configurations are available: 1/4" I.D. &times; 3/8" O.D. and 3/8" I.D. &times; 5/8" O.D., covering standard peristaltic pump head bore sizes. The female sealing cap included with each assembly caps the connector between uses, maintaining sterility. Overcaps: Vented and Solid Overcaps are secondary dust and contamination barriers placed directly on top of the BioFactory adaptor caps during culture incubation. The vented overcap contains a 0.22 &micro;m hydrophobic breathable membrane, allowing gas exchange through the cap stack while blocking particles and microbial contamination &mdash; used during active shaker or orbital incubation when the unit's primary cap is an adaptor cap rather than a vented cap. The solid overcap provides an additional dust and contamination seal with no gas exchange &mdash; used for short-term storage or transport outside the incubator. SPT-50 Silicone Hose SPT-50 silicone hose (3/8" I.D. &times; 5/8" O.D., 50 inches / 15 meters) is the bulk tubing used to connect multiple BioFactory units in series, connect BioFactory ports to peristaltic pump heads, or extend the reach of the transfer cap assembly. SPT-50 tubing is manufactured from silicone, is individually wrapped and sterile, and passes dissolution testing per BPOG (BioPhorum Operations Group) criteria per NEST product documentation, supporting its use in biopharmaceutical production environments. Premium Accessory Package NEST offers a BioFactory Accessory Premium Package that includes 2 different adaptor caps, 1 hose clamp, 1 adaptor connector, 1 vent filter (50 mm), 1 additional hose clamp, and 15 m of SPT-50 silicone hose &mdash; all individually wrapped and sterile in a single kit. This package provides all components needed to set up a closed-system BioFactory media management workflow from a single kit, reducing the number of individual part orders required. Vent Filters for Gas Exchange Management For BioFactory units using adaptor caps rather than standard vented caps, 50 mm inline vent filters (0.22 &micro;m hydrophobic PTFE membrane) are installed on the air inlet/outlet tubing line to maintain sterile gas exchange during media addition, harvest, and incubation. The vent filter is rated for high flow rates without creating excessive back-pressure that would resist liquid movement through the system. Compatibility: Narrow-Mouth BioFactory Units Only All BioFactory accessory caps and tubing assemblies are designed exclusively for the narrow-mouth BioFactory configuration. Wide-mouth BioFactory units use standard wide-mouth vent and plug seal caps only; they are not compatible with accessory cap assemblies. Confirm that your BioFactory units have narrow-mouth ports before ordering the accessory cap set. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST BioFactory&trade; accessory silicone tubing and cap assemblies are manufactured from USP Class VI silicone (TPE/silicone) and polycarbonate or PP cap bodies, individually wrapped and sterile. All components have passed dissolution testing per BPOG dissolution test criteria conducted by a certified third-party laboratory per NEST Scientific documentation. The standard Luer head connections conform to ISO 594 / GB/T 1962 for reliable compatibility with standard laboratory fittings. Vent filters use a 0.22 &micro;m hydrophobic PTFE membrane; overcaps use a 0.22 &micro;m hydrophobic membrane. NEST BioFactory accessories are carried by MBP alongside BioFactory units. 
&nbsp;
Need the right BioFactory setup? <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact MBP today</a> for technical assistance.

Biofactory System Accessories

BioFactory Cap with Silicon Tube (80cm 1/4" ID3/8" OD), Male CPC Connector with Female Sealing Cap, without BioFactory, Sterile

BioFactory Cap with Silicon Tube (80cm 3/8" ID5/8" OD), Male CPC Connector with Female Sealing Cap, without BioFactory, Sterile

BioFactory Cap with PTFE Vent Filter (0.22 μm Φ42mm), without BioFactory, Sterile

BioFactory Cap with PTFE Vent Filter (0.22 μm Φ50mm), without BioFactory

Filter/inlet & outlet tubing for BioFactory

NEST BioFactory tubing and filter cap accessories for closed aseptic liquid transfer. Four variants: silicone tube cap 1/4 inch ID (C71254-FBA080B), 3/8 inch ID (C71255-HCA080B), PTFE vent filter cap Ph42 mm (C71254-ZMC000B), PTFE vent filter Ph50 mm (C71255-ZMD000B). E-beam sterile, SAL 10e-6. 1/pk, 4/cs.

BioFactory Cap with Silicon Tube (80cm 1/4 " ID3/8" OD), Male CPC Connector with Female Sealing Cap, without BioFactory, Sterile, 1/pk, 4/cs

BioFactory Cap with Silicon Tube (80cm 3/8 " ID5/8" OD), Male CPC Connector with Female Sealing Cap, without BioFactory, Sterile, 1/pk, 4/cs

BioFactory Cap with PTFE Vent Filter(0.22 μm Φ42mm), without BioFactory , Sterile, 1/pk, 4/cs

BioFactory Cap with PTFE Vent Filter(0.22 μm Φ50mm), without BioFactory , Sterile, 1/pk, 4/cs

Cell Culture Plates

<h2 class="p2"> What are Cell Culture Plates?</h2>
 Cell culture plates are sterile, single-use polystyrene multi-well vessels used to grow, treat, and analyze cells in parallel across multiple conditions in a single vessel. All formats are E-beam sterilized to SAL 10⁻⁶, certified DNase/RNase-free and non-pyrogenic, and individually wrapped in peelable film. Choose TC-treated flat-bottom plates for adherent mammalian cell culture, assays, and transfection; choose non-treated plates for suspension, hematopoietic, and hybridoma cells; choose glass-bottom plates for live-cell confocal and high-resolution fluorescence imaging.
&nbsp;
<h2 class="p1">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="1rzg2e5" data-start="0" data-end="138"><a href="cell-culture-plates/non-treated-cell-culture-plates">Non-Treated Cell Culture Plates:</a> Designed for suspension cultures and non-adherent cell lines where cell attachment is not required.</li>
<li data-section-id="1f2n3q2" data-start="140" data-end="295"><a href="cell-culture-plates/treated-cell-culture-plates">Treated Cell Culture Plates:</a> Tissue-culture-treated surface promotes reliable attachment and growth of adherent cells for routine culture and assays.</li>
<li data-section-id="1dji5w9" data-start="297" data-end="442" data-is-last-node=""><a href="cell-culture-plates/glass-bottom-cell-culture-plates">Glass Bottom Cell Culture Plates:</a> Glass-bottom wells provide superior optical clarity for high-resolution microscopy and live-cell imaging.</li>
</ul>
<h2 class="p2">&nbsp;</h2>
<h2 class="p2">How to Choose Cell Culture Plates</h2>
 Well Count by Application Scale 6-well plates (9.4 cm&sup2;/well) are the standard for transfection, siRNA knockdown, Western blot sample preparation, luciferase reporter assays, and colony counting &mdash; any protocol that requires &ge;5 &times; 10⁶ cells per condition. 12-well plates (3.8 cm&sup2;/well) support medium-scale culture for ELISA lysate preparation, flow cytometry staining, and viral infection studies. 24-well plates (1.9 cm&sup2;/well) are versatile for drug dose-response at medium throughput, primary cell culture, and viral plaque assays. 48-well plates (0.95 cm&sup2;/well) bridge 24-well and 96-well scale for intermediate throughput experiments. 96-well plates (0.32 cm&sup2;/well) are the workhorse for high-throughput cell viability (MTS, MTT, CCK-8), cytotoxicity, ELISA, reporter gene, and compound screening assays. 384-well plates are used for ultra-high-throughput screening (HTS), where reagent cost per well drives miniaturization. TC-Treated vs. Non-Treated vs. Glass Bottom TC-treated (vacuum plasma&ndash;treated, contact angle &lt;10&deg;) flat-bottom plates support adherent mammalian cell monolayer formation &mdash; required for HeLa, HEK293 (adherent), CHO-K1, Vero, A549, MCF-7, and primary cells. Non-treated plates maintain a hydrophobic surface that prevents attachment &mdash; used for suspension cell lines (Jurkat, K562, HL-60), hybridoma culture, and spheroid/organoid formation in U-bottom wells. Glass-bottom plates combine a polystyrene plate body with a borosilicate coverglass floor (0.16&ndash;0.19 mm thickness), matching the No. 1.5 coverglass standard required for confocal, TIRF, and super-resolution microscopy. Flat vs. Round (U) vs. V-Bottom Well Geometry Flat-bottom wells provide a flat imaging surface for phase-contrast and fluorescence microscopy, uniform cell monolayer coverage, and reliable OD measurements in plate readers. Round (U)-bottom wells concentrate cells and particles to the well centre by gravity &mdash; used for rosette assays, hemagglutination inhibition (HAI), and hanging-drop spheroid culture. V-bottom wells have a sharp vertex that pellets cells tightly for wash steps in flow cytometry and immunophenotyping panels. Plate Lid and Evaporation Management NEST Scientific cell culture plates ship with a clear polystyrene lid. For high-throughput assays running 72&ndash;96 hours, edge-well evaporation in 96-well and 384-well formats can introduce artefacts; NEST Low Evaporation EDGE plates have an inter-wall design that reduces edge-well evaporation by maintaining humidity in the peripheral wells during long incubations. 
<h2 class="p2"> Specifications Context</h2>
 NEST Scientific cell culture plates are manufactured from 100% virgin polystyrene in ISO 8 (Class 10,000) cleanrooms. TC treatment uses vacuum plasma technology; the lot-release criterion is contact angle &lt;10&deg;. All plates conform to ANSI/SLAS 1-2004 through 4-2004 footprint and well-position standards (SBS format), ensuring compatibility with automated liquid handling platforms, plate readers, and automated incubators. E-beam sterilization to SAL 10⁻⁶. Plates are individually wrapped in peelable Tyvek-equivalent film for GMP cleanroom entry; lids are attached. Alphanumeric well markings (A&ndash;H rows, 1&ndash;12 columns for 96-well) are standard across all formats. NEST Scientific is ISO 9001:2015 and ISO 13485 certified with FDA registration. As of 2026, MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center. 
Pick your format, culture your cells, and get consistent results every time. <a href="https://mbpinc.net/contact-us">Contact MBP </a>and get a tailored quote today!

<h2 class="p1"> What are Non-Treated Cell Culture Plates?</h2>
 Non-treated cell culture plates are sterile, single-use multi-well polystyrene plates whose well surfaces retain the original hydrophobic, unmodified polystyrene character &mdash; no plasma, corona, or chemical treatment is applied. The hydrophobic surface (contact angle ~75&ndash;90&deg;) prevents cell attachment, making these plates the correct choice for suspension cell lines, hematopoietic cells, hybridoma culture, spheroid and organoid formation, and any experiment where surface adhesion would alter the cell phenotype or experimental readout. Non-treated plates are the correct default for all suspension-growing cell lines and for any 3D culture format &mdash; spheroids, organoids, embryoid bodies &mdash; where surface attachment is undesired.
&nbsp;
<h2 class="p1">What you will find:</h2>
&nbsp;
<ul>
<li>6, 12, 24, 48, and 96 well formats for different workflows</li>
<li>Untreated surfaces with no coating or modification</li>
<li>Clear plates for easy observation and monitoring</li>
<li>Uniform wells for consistent sample handling</li>
<li>Sterile options to maintain clean conditions</li>
<li>Lids designed to reduce contamination and evaporation</li>
</ul>
<h2 class="p1"> How to Choose Non-Treated Cell Culture Plates</h2>
 Suspension Cell Lines Requiring Non-Treated Plates Non-treated flat-bottom plates support maintenance and assays for Jurkat T-cells, K562 erythroleukemia, HL-60 promyelocytes, Raji B-cells, THP-1 monocytes, MOLM-13, and Daudi cells. Primary hematopoietic cells &mdash; PBMCs, CD34⁺ progenitors, NK cells, and bone marrow isolates &mdash; require non-treated surfaces to prevent integrin-triggered differentiation signals from TC-treated polystyrene. For hybridoma culture during HAT selection, non-treated 96-well U-bottom plates are the standard format. U-Bottom vs. Flat-Bottom for Non-Treated Plates Non-treated flat-bottom plates are the standard for suspension cell viability assays, cytotoxicity screens, and ELISA from non-adherent lysates, where wells need a uniform optical path and a flat imaging surface. Non-treated U-bottom 96-well plates concentrate non-adherent cells to the well center by gravity, producing cell pellets that are easier to aspirate during wash steps &mdash; essential for immunophenotyping panels with multiple antibody labeling and wash cycles before flow cytometry. Spheroid and 3D Culture in Non-Treated Plates Non-treated round-bottom (U-bottom) 96-well plates are widely used for hanging-drop spheroid formation and gravity-aggregated tumor spheroid culture. The hydrophobic, non-adhesive well surface combined with U-bottom geometry causes cells to aggregate at the well vertex by gravity, forming compact spheroids over 24&ndash;72 hours without scaffolding. Spheroids formed in non-treated U-bottom 96-well plates are directly measurable in the plate using a plate reader or confocal microscope with an appropriate working distance. Assay Compatibility Non-treated plates are fully compatible with cell viability reagents (CellTiter-Glo, CellTiter-Aqueous MTS, PrestoBlue), cytokine ELISA from cell supernatants, luciferase reporter assays from suspension-cell lysates, and flow cytometry sample preparation. For assays requiring a flat optical surface with minimal well-to-well distance variation, flat-bottom non-treated plates are preferred over U-bottom. Sterility and Purity Grade NEST Scientific non-treated plates are E-beam sterilized to SAL 10⁻⁶, certified DNase/RNase-free, non-pyrogenic, and individually wrapped in peelable sterile film. They are manufactured from 100% virgin polystyrene in ISO 8 cleanrooms under ISO 9001:2015 and ISO 13485 quality management. No additional surface chemistry or coating is applied; the non-treated specification is confirmed by water contact angle measurement per NEST lot release protocol. 
<h2 class="p1"> Specifications Context</h2>
 NEST Scientific non-treated plates are manufactured from 100% virgin polystyrene in ISO 8 cleanrooms; the non-treated well surface has a water contact angle of ~75&ndash;90&deg; (hydrophobic) confirmed per lot release. All plates conform to ANSI/SLAS SBS footprint standards (127.76 &times; 85.48 mm), ensuring compatibility with automated liquid handlers and plate readers. Alphanumeric well coordinates are printed on all plates &ge;12-well format. Package box double functions as a reagent reservoir for multi-channel pipette operations per NEST design. As of 2026, NEST Scientific non-treated plates in all core formats are stocked by MBP. 
&nbsp;
Use non-treated plates when you want full control over how your cells interact with the surface. Keep conditions simple. Keep results predictable. <a href="https://mbpinc.net/contact-us">Contact the MBP team</a> now to get a tailored quote.

Non-Treated Cell Culture Plates

DataSheet

Flat Bottom Cell Culture Plates, Sterile, Non-Treated

NEST Scientific Non-Treated Flat-Bottom Cell Culture Plate is a sterile 6-well flat-bottom cell culture plate with non-treated hydrophobic polystyrene surface. Features a low-evaporation lid and corner feet for microscopy. Individually wrapped, 1/pk, 50/cs — for suspension culture, spheroid formation, and applications where cell attachment must be prevented. Available in 6-well (703011), 12-well (712011), 24-well (702011), 48-well (748011), and 96-well (701011). All sterile, non-treated, 1/pk.

6 Well Cell Culture Plate,Flat,Non-Treated, sterile 1/pk, 50/cs

12 Well Cell Culture Plate, Flat, Non-Treated, sterile 1/pk, 50/cs

24 Well Cell Culture Plate, Flat, Non-Treated, sterile 1/pk, 50/cs

48 Well Cell Culture Plate, Flat, Non-Treated, sterile 1/pk, 50/cs

96 Well Cell Culture Plate, Flat, Non-Treated, sterile 1/pk, 100/cs

<h2 dir="ltr">What are PC Erlenmeyer Flasks?</h2>
 PC (polycarbonate) Erlenmeyer flasks are autoclavable, shatter-proof, optically clear shake flasks manufactured from USP Class VI medical-grade polycarbonate for suspension cell culture, microbial fermentation, and media preparation on orbital shakers. Polycarbonate provides a working temperature range of &minus;135 &deg;C to +135 &deg;C and withstands 5&ndash;10 autoclave cycles at 121 &deg;C &mdash; the defining advantage of PC over PETG for labs that steam-sterilize media or require reusable, re-sterilizable shake flasks. PC Erlenmeyer flasks are the correct choice when autoclavability, reuse, or processing at elevated temperature (&gt;60 &deg;C) is required; for single-use BPA-free workflows, PETG Erlenmeyer flasks are the alternative.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Vent Filter Caps featuring 0.22&micro;m hydrophobic membranes to facilitate continuous, sterile gas exchange while preventing contamination and evaporation.
</li>
<li dir="ltr" aria-level="1">
Seal Caps designed for airtight liquid storage, transport, and non-aerated incubation steps.
</li>
<li dir="ltr" aria-level="1">
Flat Bottom Flasks optimized for uniform heating and stability on standard orbital shakers.
</li>
<li dir="ltr" aria-level="1">
Baffled Bottom Flasks engineered with integrated "flutes" to increase turbulence and oxygen mass transfer, ideal for high-density yeast and bacterial cultures.
</li>
<li dir="ltr" aria-level="1">
Volume Scalability with a comprehensive range from 125mL pilots to 5L high-efficiency production vessels, allowing for a seamless transition from bench-scale research to bulk bioprocessing.
</li>
<li dir="ltr" aria-level="1">
High-Efficiency Wide-Mouth Designs facilitate&nbsp;rapid filling and easy access for large-volume pipetting and automated dispensers.
</li>
</ul>
<h2 dir="ltr"> How to Choose PC Erlenmeyer Flasks</h2>
 Flat Base vs. Baffled Base for Your Cell Line Flat-base PC flasks produce laminar rotational flow during orbital shaking, providing low-shear mixing appropriate for shear-sensitive primary cells, some hybridoma lines, and media preparation. Baffled-base PC flasks generate turbulence during shaking that substantially increases dissolved oxygen (DO) transfer &mdash; critical for fast-growing CHO-S, HEK293-F, Expi293, bacterial (E. coli, B. subtilis), and yeast cultures where DO depletion limits cell density and productivity. NEST Scientific offers both base geometries across all standard volumes from 125 mL to 3,000 mL, with High Efficiency baffled formats extending to 5,000 mL for production-scale operations. Vented Cap vs. Plug Seal Cap Vented caps (0.22 &micro;m hydrophobic PTFE membrane, HDPE housing) allow continuous gas exchange during shaker culture while maintaining a sterile barrier. The NEST oversized vented cap membrane provides greater gas transfer area than standard-dimension vented caps, supporting high-density cultures. Plug seal caps are gas-impermeable HDPE caps used for static media storage in PC flasks, transport between incubators, and autoclave cycles (caps must be loosened 1&ndash;1.5 turns before autoclaving to prevent pressure buildup). Never use a sealed plug cap on a flask in an orbital shaker incubator &mdash; medium acidification and DO depletion occur within hours. Volume Selection and Fill Ratio NEST Scientific PC flasks are available in 125 mL, 250 mL, 500 mL, 1,000 mL, 2,000 mL, 3,000 mL, and 5,000 mL (High Efficiency). Working fill for shaker culture: 20&ndash;25% of nominal volume. Working volumes: 25&ndash;30 mL in 125 mL; 50&ndash;60 mL in 250 mL; 100&ndash;125 mL in 500 mL; 200&ndash;250 mL in 1,000 mL; 400&ndash;500 mL in 2,000 mL. Scale-up from T-flask to Erlenmeyer: transition from T75 (15&ndash;25 mL static) to a 125 mL PC baffled flask when moving to suspension-adapted cell lines requiring orbital shaking. Autoclave Protocol for PC Flasks PC Erlenmeyer flasks can be autoclaved at 121 &deg;C for 15&ndash;20 minutes (standard gravity cycle). Loosen cap 1&ndash;1.5 turns before autoclaving; retighten after cooling. Repeated autoclaving causes progressive hydrolytic degradation of carbonate linkages &mdash; inspect for crazing, discoloration, or dimensional distortion after each cycle and retire flask at first sign of damage. Do not use autoclaved PC flasks for vacuum applications. BPA Consideration Polycarbonate contains bisphenol A (BPA) as an inherent part of its monomer chemistry. For applications involving estrogen receptor&ndash;positive cell lines (MCF-7, T47D, ZR-75), primary endocrine cells, or long-term culture runs exceeding 14 days, PETG Erlenmeyer flasks (BPA-free) are the safer choice. For standard bacterial, yeast, CHO, and HEK293 suspension culture, trace BPA from USP Class VI PC does not affect results in published culture protocols. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific PC Erlenmeyer flasks are manufactured from USP Class VI medical-grade PC resin in ISO 8 cleanrooms, gamma sterilized per ISO 11137 to SAL 10⁻⁶, and individually wrapped in sterile packaging. PC tensile strength: 55&ndash;75 MPa; maintains impact resistance &gt;50 J/m at &minus;40 &deg;C. Vented cap 0.22 &micro;m hydrophobic PTFE membrane confirmed non-pyrogenic. Flask base diameter and neck geometry are designed for compatibility with standard orbital shaker clips from Infors HT, New Brunswick Innova, and Thermo Scientific MaxQ platforms &mdash; confirm flask O.D. against your specific shaker clamp before bulk ordering. NEST Scientific PC Erlenmeyer flasks up to 5 L (High Efficiency line) are stocked by MBP.
&nbsp;
Connect with the <a href="https://mbpinc.net/contact-us" title="MBP team" style="color: #236fa1; text-decoration: underline;">MBP team</a> today to secure the exact volumes and cap configurations required for your laboratory&rsquo;s next breakthrough.

PC Erlenmeyer Flasks

Flat Bottom PC Conical Erlenmeyer Flasks

NEST Scientific flat-bottom PC Erlenmeyer flasks for shaker culture, ISO 10993 medical-grade PC, E-beam SAL 10⁻⁶, autoclavable to 121°C. Eight variants: 125/250/500/1,000 mL × seal cap (781101/782101/783101/784101) or PTFE vent cap (781111/782111/783111/784111). 6–24/cs.

125 mL PC Conical Erlenmeyer Flasks, Flat Bottom, Seal Caps, Sterile,1/pk, 24/cs

250 mL PC Conical Erlenmeyer Flasks, Flat Bottom, Seal Caps, Sterile,1/pk, 12/cs

500 mL PC Conical Erlenmeyer Flasks, Flat Bottom, Seal Caps, Sterile,1/pk, 12/cs

1000 mL PC Conical Erlenmeyer Flasks, Flat Bottom, Seal Caps, Sterile,1/pk, 6/cs

125 mL PC Conical Erlenmeyer Flasks, Flat Bottom, Vent Filter Caps, Sterile,1/pk, 24/cs

250 mL PC Conical Erlenmeyer Flasks, Flat Bottom, Vent Filter Caps, Sterile,1/pk, 12/cs

500 mL PC Conical Erlenmeyer Flasks, Flat Bottom, Vent Filter Caps, Sterile,1/pk, 12/cs

1000 mL PC Conical Erlenmeyer Flasks, Flat Bottom, Vent Filter Caps, Sterile,1/pk, 6/cs

Flat Bottom PC High Efficient Erlenmeyer Flasks

NEST Flat-Bottom PC High Efficiency Erlenmeyer Flask: 2 L flat-bottom polycarbonate (ISO 10993) high-efficiency Erlenmeyer flask. E-beam sterile SAL 10&minus;6, DNase/RNase-free, autoclavable to 121&deg;C. Plug seal or vent cap. 1/pk, 4/cs. For mammalian and insect cell culture on orbital shakers. Available in 2L (785101 seal cap, 785111 vent cap), 3L (786101 seal cap, 786111 vent cap), and 5L (787001 seal cap, 787011 vent cap).

2L PC High Efficient Erlenmeyer Flasks, Flat Base, Seal Cap, Sterile, 1/pk, 4/cs

2L PC High Efficient Erlenmeyer Flasks, Flat Base, Vent Filter Cap, Sterile, 1/pk, 4/cs

3L PC High Efficient Erlenmeyer Flasks, Flat Base, Seal Cap, Sterile, 1/pk, 4/cs

3L PC High Efficient Erlenmeyer Flasks, Flat Base, Vent Filter Cap, Sterile, 1/pk, 4/cs

5L PC High Efficient Erlenmeyer Flasks, Flat Base, Seal Cap, Sterile, 1/pk, 4/cs

5L PC High Efficient Erlenmeyer Flasks, Flat Base, Vent Filter Cap, Sterile, 1/pk, 4/cs

<h2 dir="ltr"> What are PETG Erlenmeyer Flasks?</h2>
 PETG (polyethylene terephthalate glycol) Erlenmeyer flasks are single-use, sterile, BPA-free conical shake flasks manufactured from glycol-modified PET for suspension cell culture, microbial fermentation, and media preparation on orbital shakers. PETG offers optical clarity comparable to PC, a working temperature range of &minus;80 &deg;C to +60 &deg;C, superior impact resistance at low temperatures versus standard PET, and a BPA-free composition &mdash; advantages over PC in single-use workflows where autoclaving is not required, and BPA-sensitive cell culture applications are involved.&nbsp; PETG Erlenmeyer flasks are the correct choice for high-throughput single-use suspension culture, BPA-free applications, and labs that want to eliminate autoclave and washing steps in their shake flask workflow.
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<h2 dir="ltr">What you will find:</h2>
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125mL PETG Erlenmeyer Flasks for Screening Studies: Perfect for small-scale media optimization and high-speed orbital shaking.
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250mL PETG Erlenmeyer Flasks for Routine Expansion: Provides a stable environment for consistent cell growth and passage maintenance.
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500mL PETG Erlenmeyer Flasks for Batch Production: Optimized for increasing cell densities while maintaining superior gas exchange.
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1000mL PETG Erlenmeyer Flasks for Large-Scale Recovery: Engineered for high-volume bioprocessing and bulk reagent preparation.
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<h2 dir="ltr"> How to Choose PETG Erlenmeyer Flasks</h2>
 PETG vs. PC for Shake Flask Culture PETG flasks are single-use and cannot be autoclaved (maximum temperature +60 &deg;C). PC flasks can be autoclaved 5&ndash;10 times and have a broader temperature range (&minus;135 &deg;C to +135 &deg;C). For high-throughput suspension culture operations processing many flasks per week, single-use PETG eliminates all washing, autoclave queuing, and glassware inspection steps &mdash; reducing hands-on time and contamination risk from reuse. For labs with lower throughput that prepare media in-house via autoclave, PC remains the cost-effective choice. PETG is BPA-free; PC contains BPA. Cell Line Compatibility PETG Erlenmeyer flasks are validated for CHO-S, HEK293-F, Expi293, Jurkat, K562, Sf9 (Spodoptera frugiperda), Hi5 (Trichoplusia ni), S. cerevisiae, P. pastoris, and E. coli suspension culture. The non-treated PETG surface prevents inadvertent cell adhesion and is compatible with all standard suspension culture media, including FreeStyle CHO, Expi293, Sf-900, and ESF 921 insect cell media. PETG is chemically resistant to DMSO, ethanol, isopropanol, and standard aqueous buffers. Baffled vs. Flat Base for PETG Flasks Baffled-base PETG Erlenmeyer flasks are the standard for oxygen-demanding suspension cultures: CHO, HEK293-F, bacterial, and insect cell lines. The baffles generate turbulent flow during orbital shaking, raising dissolved oxygen and reducing cell sedimentation. Flat-base PETG flasks are used for media preparation, gentle mixing, and shear-sensitive cell lines where turbulent conditions cause cell damage. NEST Scientific offers baffled and flat-base versions in both 125 mL and 1,000 mL. Vented Cap Gas Exchange NEST Scientific PETG Erlenmeyer flasks ship with a 0.22 &micro;m hydrophobic PTFE membrane vented cap in an HDPE housing with an oversized vent area. For suspension culture with 20&ndash;25% fill volume, vented caps at standard orbital speeds (100&ndash;150 RPM) provide adequate oxygen mass transfer to support mammalian cell densities up to approximately 5 &times; 10⁶ cells/mL. For cultures targeting higher densities or with very high specific oxygen uptake rates (sOUR), confirm dissolved oxygen by inline monitoring before scaling further in PETG flasks. Storage of Unused Sterile Flasks Store unused NEST Scientific PETG Erlenmeyer flasks at room temperature (15&ndash;30 &deg;C) in the original sealed sterile bag, away from UV light. Shelf life is typically 3 years from the production date per the lot certificate. Inspect the bag seal before use; a compromised seal invalidates sterility. Remove from the bag inside a biosafety cabinet immediately before inoculation. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific PETG Erlenmeyer flasks are manufactured from USP Class VI medical-grade PETG in ISO 8 cleanrooms. E-beam sterilized to SAL 10⁻⁶; each lot certified DNase/RNase-free, pyrogen-free, non-cytotoxic. Temperature range: &minus;80 &deg;C to +60 &deg;C. HDPE cap with 0.22 &micro;m PTFE hydrophobic membrane; oversized vent area for improved gas transfer. NEST Scientific is ISO 9001:2015 and ISO 13485 certified; FDA registered. NEST Scientific PETG Erlenmeyer flasks in 125 mL, 250 mL, 500 mL, and 1,000 mL with baffled and flat-base options are stocked by MBP. 
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Source high-quality laboratory supplies with Molecular Biology Products Inc. <a href="https://mbpinc.net/contact-us">Connect with our specialists</a> for customized quotations.

PETG Erlenmeyer Flasks

PETG Erlenmeyer Flask, Flat base

Flat Bottom PETG Erlenmeyer Flasks

Flat Bottom PETG Erlenmeyer Flask by NEST Scientific — 125 mL flat-bottom PETG Erlenmeyer flask with USP Class VI plug seal cap. E-beam sterile (SAL 10-6), cGMP/ISO 13485 facility, pyrogen-free, DNase/RNase-free. 1/pk, 24/cs. Available in 125 mL (781001/781011), 250 mL (782001/782011), 500 mL (783001/783011), and 1000 mL (784001/784011); seal or vent cap.