Name: Flat Bottom PETG Erlenmeyer Flasks
SKU: 781001
Availability: InStock

Erlenmeyer Flasks

<h2 dir="ltr">What are Cell Culture Erlenmeyer Flasks?</h2>
 Cell culture Erlenmeyer flasks (also called shake flasks or conical flasks) are single-use, sterile plastic vessels with a conical body, flat base, and narrow neck used for suspension cell culture on orbital shakers and incubator shakers, microbial culture, and media preparation. Unlike rectangular T-flasks designed for static monolayer culture, Erlenmeyer flasks are engineered for shaking: their wide conical base maximizes liquid surface area for oxygen transfer, while the narrow neck minimizes evaporation and contamination risk. Choose PC Erlenmeyer flasks for autoclavable reuse and high-temperature media preparation; choose PETG for single-use suspension culture and processes requiring BPA-free materials.
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<h2 dir="ltr">What you will find:</h2>
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<a href="erlenmeyer-flasks/petg-erlenmeyer-flasks">PETG Erlenmeyer flasks: </a>strong choice for cell culture applications that need excellent clarity, reliable handling, and convenient single-use performance. They are lightweight, durable, and ideal for shaker culture, media preparation, and general suspension cell workflows.
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<a href="erlenmeyer-flasks/pc-erlenmeyer-flasks">PC Erlenmeyer flasks: </a>built for applications that require greater strength and higher impact resistance. They are well-suited for demanding lab environments, including vigorous shaking, transport, and workflows where added durability is important.
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<li dir="ltr" aria-level="1"><a href="https://mbpinc.net/consumables/cell-culture/erlenmeyer-flasks/erlenmeyer-flask-accessories">Erlenmeyer Flask Accessories: </a>Includes bi-directional, inverted, and multifunctional transfer caps, plus silicon tube assemblies for 2 L, 3 L, 3 L wide-mouth, and 5 L high-efficiency flasks, supporting sterile transfer and closed-system workflows.</li>
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<h2 dir="ltr"> How to Choose Erlenmeyer Flasks for Cell Culture</h2>
 PC vs. PETG Material PC (polycarbonate) Erlenmeyer flasks are autoclavable at 121 &deg;C (typically 5&ndash;10 cycles), shatter-proof, crystal-clear, and rated from &minus;135 &deg;C to +135 &deg;C. PC is ideal for labs that sterilize media or wash in-house. PC contains BPA. PETG Erlenmeyer flasks are single-use, BPA-free, rated from &minus;80 &deg;C to +60 &deg;C, and cannot be autoclaved. They provide a contamination-free, cleaning-free alternative to glass and PC for high-throughput suspension culture operations. Flat Base vs. Baffled Base Flat-base Erlenmeyer flasks provide gentler swirling for slow-growth cell lines and media mixing. Baffled-base flasks have three or four angled internal baffles that interrupt the swirling flow, increasing turbulence, raising dissolved oxygen (DO) by up to 2&times; compared to flat-base flasks at the same orbital speed, and reducing cell sedimentation. Baffled flasks are the standard for CHO-S, HEK293-F, Sf9, microbial, and yeast cultures requiring high oxygen transfer rates. NEST Scientific offers baffled and flat-base versions in both PC and PETG across all core volumes. Vented vs. Plug Seal Cap Vented caps contain a 0.22 &micro;m hydrophobic PTFE membrane that allows free O₂/CO₂ gas exchange while excluding microbial contamination. The NEST vented cap features an oversized vent area that improves gas exchange beyond standard-size membrane caps, supporting oxygen-demanding suspension cultures at higher cell densities. Plug seal caps create a fully sealed environment for media storage, static incubation, or transport outside the incubator. Always use vented caps for actively growing suspension cultures on an orbital shaker. Volume and Fill Ratio Erlenmeyer flasks should be filled to no more than 20&ndash;25% of the nominal flask volume for suspension shaker culture to maintain adequate headspace for oxygen transfer. A 500 mL flask running at 20% fill holds 100 mL of culture; a 1,000 mL flask holds 200 mL. Overfilling reduces oxygen transfer efficiency and creates foam that blocks the vented cap membrane. For media storage (no shaking), the fill volume can approach 80% of nominal. Shaker Compatibility NEST Scientific Erlenmeyer flasks (125 mL&ndash;3,000 mL) are compatible with standard laboratory orbital shakers, including those from Infors HT, New Brunswick Scientific (Innova), and Thermo Scientific MaxQ. Confirm flask neck O.D. and base diameter against your shaker's flask holder clips or clamp system before ordering large quantities. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific PC Erlenmeyer flasks are manufactured from USP Class VI medical-grade polycarbonate, gamma sterilized per ISO 11137 to SAL 10⁻⁶, and individually wrapped in sterile packaging. PETG Erlenmeyer flasks are E-beam sterilized and certified DNase/RNase-free, pyrogen-free per lot. Vented cap PTFE membranes are 0.22 &micro;m with an oversized vent area that increases gas exchange efficiency vs. standard-diameter membrane caps. NEST Scientific is ISO 9001:2015 and ISO 13485 certified with FDA registration. Flask catalog sizes extend to high-efficiency 2 L and 5 L PC formats (NEST High Efficiency Erlenmeyer Flask line) for production-scale suspension culture. MBP stocks the NEST Scientific Erlenmeyer flask line across all major volumes and configurations. 
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Support accurate mixing and culturing with premium Erlenmeyer flasks designed for modern laboratories. <a href="https://www.mbpinc.net/contact-us" title="Contact the MBP Team" style="color: #236fa1; text-decoration: underline;">Contact the MBP team</a>&nbsp;for a formal quote.

<h2 dir="ltr"> What are PETG Erlenmeyer Flasks?</h2>
 PETG (polyethylene terephthalate glycol) Erlenmeyer flasks are single-use, sterile, BPA-free conical shake flasks manufactured from glycol-modified PET for suspension cell culture, microbial fermentation, and media preparation on orbital shakers. PETG offers optical clarity comparable to PC, a working temperature range of &minus;80 &deg;C to +60 &deg;C, superior impact resistance at low temperatures versus standard PET, and a BPA-free composition &mdash; advantages over PC in single-use workflows where autoclaving is not required, and BPA-sensitive cell culture applications are involved.&nbsp; PETG Erlenmeyer flasks are the correct choice for high-throughput single-use suspension culture, BPA-free applications, and labs that want to eliminate autoclave and washing steps in their shake flask workflow.
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<h2 dir="ltr">What you will find:</h2>
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125mL PETG Erlenmeyer Flasks for Screening Studies: Perfect for small-scale media optimization and high-speed orbital shaking.
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250mL PETG Erlenmeyer Flasks for Routine Expansion: Provides a stable environment for consistent cell growth and passage maintenance.
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500mL PETG Erlenmeyer Flasks for Batch Production: Optimized for increasing cell densities while maintaining superior gas exchange.
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1000mL PETG Erlenmeyer Flasks for Large-Scale Recovery: Engineered for high-volume bioprocessing and bulk reagent preparation.
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<h2 dir="ltr"> How to Choose PETG Erlenmeyer Flasks</h2>
 PETG vs. PC for Shake Flask Culture PETG flasks are single-use and cannot be autoclaved (maximum temperature +60 &deg;C). PC flasks can be autoclaved 5&ndash;10 times and have a broader temperature range (&minus;135 &deg;C to +135 &deg;C). For high-throughput suspension culture operations processing many flasks per week, single-use PETG eliminates all washing, autoclave queuing, and glassware inspection steps &mdash; reducing hands-on time and contamination risk from reuse. For labs with lower throughput that prepare media in-house via autoclave, PC remains the cost-effective choice. PETG is BPA-free; PC contains BPA. Cell Line Compatibility PETG Erlenmeyer flasks are validated for CHO-S, HEK293-F, Expi293, Jurkat, K562, Sf9 (Spodoptera frugiperda), Hi5 (Trichoplusia ni), S. cerevisiae, P. pastoris, and E. coli suspension culture. The non-treated PETG surface prevents inadvertent cell adhesion and is compatible with all standard suspension culture media, including FreeStyle CHO, Expi293, Sf-900, and ESF 921 insect cell media. PETG is chemically resistant to DMSO, ethanol, isopropanol, and standard aqueous buffers. Baffled vs. Flat Base for PETG Flasks Baffled-base PETG Erlenmeyer flasks are the standard for oxygen-demanding suspension cultures: CHO, HEK293-F, bacterial, and insect cell lines. The baffles generate turbulent flow during orbital shaking, raising dissolved oxygen and reducing cell sedimentation. Flat-base PETG flasks are used for media preparation, gentle mixing, and shear-sensitive cell lines where turbulent conditions cause cell damage. NEST Scientific offers baffled and flat-base versions in both 125 mL and 1,000 mL. Vented Cap Gas Exchange NEST Scientific PETG Erlenmeyer flasks ship with a 0.22 &micro;m hydrophobic PTFE membrane vented cap in an HDPE housing with an oversized vent area. For suspension culture with 20&ndash;25% fill volume, vented caps at standard orbital speeds (100&ndash;150 RPM) provide adequate oxygen mass transfer to support mammalian cell densities up to approximately 5 &times; 10⁶ cells/mL. For cultures targeting higher densities or with very high specific oxygen uptake rates (sOUR), confirm dissolved oxygen by inline monitoring before scaling further in PETG flasks. Storage of Unused Sterile Flasks Store unused NEST Scientific PETG Erlenmeyer flasks at room temperature (15&ndash;30 &deg;C) in the original sealed sterile bag, away from UV light. Shelf life is typically 3 years from the production date per the lot certificate. Inspect the bag seal before use; a compromised seal invalidates sterility. Remove from the bag inside a biosafety cabinet immediately before inoculation. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific PETG Erlenmeyer flasks are manufactured from USP Class VI medical-grade PETG in ISO 8 cleanrooms. E-beam sterilized to SAL 10⁻⁶; each lot certified DNase/RNase-free, pyrogen-free, non-cytotoxic. Temperature range: &minus;80 &deg;C to +60 &deg;C. HDPE cap with 0.22 &micro;m PTFE hydrophobic membrane; oversized vent area for improved gas transfer. NEST Scientific is ISO 9001:2015 and ISO 13485 certified; FDA registered. NEST Scientific PETG Erlenmeyer flasks in 125 mL, 250 mL, 500 mL, and 1,000 mL with baffled and flat-base options are stocked by MBP. 
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Source high-quality laboratory supplies with Molecular Biology Products Inc. <a href="https://mbpinc.net/contact-us">Connect with our specialists</a> for customized quotations.

PETG Erlenmeyer Flasks

DataSheet

PETG Erlenmeyer Flask, Flat base

Flat Bottom PETG Erlenmeyer Flasks

Flat Bottom PETG Erlenmeyer Flask by NEST Scientific — 125 mL flat-bottom PETG Erlenmeyer flask with USP Class VI plug seal cap. E-beam sterile (SAL 10-6), cGMP/ISO 13485 facility, pyrogen-free, DNase/RNase-free. 1/pk, 24/cs. Available in 125 mL (781001/781011), 250 mL (782001/782011), 500 mL (783001/783011), and 1000 mL (784001/784011); seal or vent cap.