PCR Plates

<h2 data-path-to-node="3">What Are PCR Plates?</h2>
 PCR plates are single-use, thin-wall polypropylene multi-well vessels designed for the thermal cycling demands of PCR, qPCR, RT-PCR, and genotyping. The 96-well format (12 columns &times; 8 rows) is the standard for most research and clinical labs; 384-well plates serve ultra-high-throughput applications. Well volumes are 0.2 mL (standard profile) for conventional block cyclers or 0.1 mL (low profile) for fast-block instruments. The skirt &mdash; the rigid plastic border surrounding the well array &mdash; determines automation compatibility, cycler lid clearance, and plate rigidity.&nbsp;
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li data-section-id="1xmdc3x" data-start="0" data-end="87"><a href="https://mbpinc.net/consumables/pcr-consumables/pcr-plates/semi-skirted-plates">Semi-skirted PCR plates: </a>for a balance of plate rigidity and instrument compatibility.</li>
<li data-section-id="y5pq2h" data-start="89" data-end="156"><a href="https://mbpinc.net/consumables/pcr-consumables/pcr-plates/hard-shell-pcr-plates">Hard shell PCR plates: </a>designed with a reinforced plate structure.</li>
<li data-section-id="8t808z" data-start="158" data-end="243"><a href="https://mbpinc.net/consumables/pcr-consumables/pcr-plates/skirted-pcr-plates">Skirted PCR plates:</a> featuring a full peripheral skirt for enhanced handling support.</li>
<li data-section-id="1d0aj4c" data-start="245" data-end="343" data-is-last-node=""><a href="https://mbpinc.net/consumables/pcr-consumables/pcr-plates/non-skirted-pcr-plates">Non-skirted PCR plates:</a> with a compact design for applications requiring minimal plate footprint.</li>
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<h2 data-path-to-node="3">How to Choose PCR Plates</h2>
 Skirt Format Fully-skirted plates have a full-height ANSI/SBS-compliant border (127.76 &times; 85.47 mm footprint) and are required for robotic liquid handlers (Hamilton STAR, Tecan EVO, PerkinElmer JANUS) and automated stackers. Semi-skirted plates have a partial skirt providing enough rigidity for multichannel pipetting while maintaining thermal cycler block compatibility across a wider range of instruments. Non-skirted plates have no border and fit virtually any cycler block, including instruments whose compression lids would be obstructed by a full skirt. Well Profile: Standard vs Low-Profile Standard-profile 0.2 mL wells are used with conventional (tall) block cyclers. Low-profile 0.1 mL wells are required for fast-block instruments, including the Applied Biosystems QuantStudio, Bio-Rad CFX96 Fast, and Roche LightCycler 480, where the reduced well depth shortens the thermal path to the sample. Colour: Clear, White, or Black Clear plates allow visual inspection of well volumes and are the default for end-point PCR. White plates increase qPCR sensitivity in bottom-read optical systems by reflecting fluorescence emission back through the detector. Black plates reduce background fluorescence in probe-based assays where cross-well optical interference is a concern. Sealing Compatibility Skirted and semi-skirted plates seal reliably with adhesive films and heat sealers; the rigid skirt supports even film application and reduces edge-well evaporation. Non-skirted plates require cap mats or adhesive seals that adhere directly to the well rim. 
<h2 data-path-to-node="3"> Specifications Context</h2>
 PCR plates are moulded from virgin, low-extractable medical-grade polypropylene; well walls are uniformly thin (typically 0.25&ndash;0.35 mm) to maximise thermal conductivity. ANSI/SBS footprint standard (127.76 &times; 85.47 mm) applies to skirted plates for instrument interoperability. Plates are certified DNase-free, RNase-free, human DNA-free, and PCR inhibitor-free per lot. Cut corners (typically at H1/A12 position) and alpha-numeric well labelling aid plate orientation.Thermo Fisher SuperPlate skirted formats are the leading references for robotic applications; semi-skirted plates from Sorenson and equivalent lines serve the broad research market.&nbsp; 
<a href="https://mbpinc.net/contact-us">Connect with the MBP team </a>today to request a quote and find the perfect PCR plate configuration for your lab's specific needs.

<h2>What Are Semi-Skirted PCR Plates?</h2>
 Semi-skirted PCR plates have a partial plastic border extending approximately half the height of a fully skirted plate, surrounding part of the well array. This partial skirt adds enough structural rigidity to support multichannel pipetting. It provides a surface for labelling, while keeping the overall plate height low enough for compatibility with most thermal cycler compression lids that a full skirt would obstruct. Semi-skirted plates represent the most common format in routine qPCR and genotyping labs, where fully automated robotic handling is not required. Semi-skirted is the default choice for most routine PCR and qPCR labs that mix manual pipetting with occasional automated steps. 
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<h2 data-start="476" data-end="501">What you will find: </h2>
<ul data-start="502" data-end="1108">
<li style="color: #000000;" data-section-id="47u9b8" data-start="502" data-end="589">Hard shell 384-well PCR plates &ndash; enhanced rigidity for high-throughput applications</li>
<li style="color: #000000;" data-section-id="1oohzes" data-start="590" data-end="670">Semi-skirted, 96-well PCR plates &ndash; balanced design for routine PCR workflows</li>
<li style="color: #000000;" data-section-id="1n8sxu4" data-start="671" data-end="748">Hard Shell 96-well PCR plates &ndash; improved structural support and alignment</li>
<li style="color: #000000;" data-section-id="1gxz5rn" data-start="749" data-end="830">Globe Scientific Half Skirt PCR Plates &ndash; reliable option from a trusted brand</li>
<li style="color: #000000;" data-section-id="wcryl4" data-start="831" data-end="897">PCR Plates, Semi Skirt &ndash; versatile plates for standard lab use</li>
<li style="color: #000000;" data-section-id="dlek6q" data-start="898" data-end="985">96-well Duo Material PCR plate &ndash; optimized materials for performance and durability</li>
<li style="color: #000000;" data-section-id="4vb9o1" data-start="986" data-end="1108">Semi-Skirt 96 Plate, Clear Polypropylene, Non-Sterile &ndash; clear construction for visibility and general applications</li>
</ul>
<h2> How to Choose Semi-Skirted PCR Plates</h2>
 Well Profile and Volume Low-profile 0.1 mL semi-skirted plates are the standard for qPCR on fast-block instruments, including Bio-Rad CFX96, Applied Biosystems StepOnePlus, and Roche LightCycler 480. Standard-profile 0.2 mL wells work with conventional block cyclers and accommodate larger reaction volumes for cloning or high-concentration template workflows. Colour: Clear vs White Clear semi-skirted plates suit end-point PCR and top-read qPCR systems. White semi-skirted plates increase fluorescence signal in bottom-read qPCR optical configurations. Thermo Fisher offers white semi-skirted formats for this application. Cut Corner for Orientation Semi-skirted plates typically feature a notched or cut corner at the A12 position for plate orientation &mdash; a feature originating in sequencing plate formats and now standard across most qPCR-compatible semi-skirted plates. Confirm the cut corner position against your instrument's plate alignment specification. Sealing The partial skirt of semi-skirted plates supports adhesive film sealing and is compatible with most heat sealers. The skirt also reduces edge-well evaporation compared to non-skirted plates, improving Ct uniformity across plate positions. Robotic Compatibility Semi-skirted plates provide enough grip surface for some robotic arm designs and are listed as compatible with select liquid handlers; however, for demanding robotic workflows with gripper systems or conveyor belts, fully skirted ANSI/SBS-compliant plates remain the correct specification. 
<h2> Specifications Context</h2>
 Semi-skirted 96-well plates are moulded from virgin polypropylene with uniformly thin walls (0.25&ndash;0.35 mm) for consistent thermal conductivity. The partial skirt height is approximately 50% of a full ANSI/SBS skirt height, placing the plate between non-skirted and fully skirted in both rigidity and cycler compatibility. All plates are independently certified DNase-free, RNase-free, human DNA-free, and PCR inhibitor-free per lot.&nbsp; 
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Let <a href="https://mbpinc.net/contact-us">the MBP team&nbsp;</a>help you choose the right plate format to match your workflow and instrumentation.

Semi-Skirted Plates

<h2 data-path-to-node="1"> What Are Skirted PCR Plates?</h2>
 Skirted PCR plates are 96-well polypropylene thermal cycling plates with a full-height plastic border &mdash; the skirt &mdash; surrounding the well array. The skirt raises the overall plate height to match the ANSI/SBS microplate footprint standard (127.76 &times; 85.47 mm external dimensions), creating a uniform grip surface that robotic arms, plate grippers, and automated stackers can handle reliably. They are the required format for liquid-handling automation platforms and high-throughput PCR workflows where consistent plate positioning is critical. Choose fully skirted plates when your workflow involves robotic liquid handling, automated stackers, or plate readers requiring SBS-compliant positioning.
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<h2 data-path-to-node="4">What You Will Find: </h2>
<ul data-path-to-node="5">
<li>
Hard Shell 384-well PCR Plates<a href="https://www.google.com/search?q=https://mbpinc.net/product-category/consumables/pcr-consumables/pcr-plates/hard-shell-pcr-plates/" style="color: #000000;"> </a>&ndash; The industry standard for high-throughput screening and automated liquid handling.
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<li style="color: #000000;">
Hard Shell 96-well PCR Plates &ndash; Combining rigidity with a 96-well format for routine yet high-precision workflows.
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<li style="color: #000000;">
Globe Scientific Full Skirt PCR Plates &ndash; Reliable, automation-friendly plates designed for consistent thermal cycling.
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<li style="color: #000000;">
Full Skirt PCR Plates<a _ngcontent-ng-c3793280901="" target="_blank" rel="noopener" externallink="" _nghost-ng-c3773094726="" jslog="197247;track:generic_click,impression,attention;BardVeMetadataKey:[[&quot;r_98f8600e63dbeb30&quot;,&quot;c_c4e8a23c27447c36&quot;,null,&quot;rc_fa92ce727d8e45fc&quot;,null,null,&quot;en&quot;,null,1,null,null,1,0]]" href="https://www.google.com/search?q=https://mbpinc.net/product-category/consumables/pcr-consumables/pcr-plates/skirted-pcr-plates/" class="ng-star-inserted" data-hveid="0" decode-data-ved="1" data-ved="0CAAQ_4QMahgKEwjZx-625v-TAxUAAAAAHQAAAAAQsAQ" style="color: #000000;"></a> &ndash; Features a full perimeter skirt for superior stability and easy gripping during automated handling.
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<li style="color: #000000;">
96-well Duo Material PCR Plate&ndash; Engineered with dual-material technology to eliminate plate warping and bowing.
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<li style="color: #000000;">
Skirted 96 Plate, Non-Sterile, Clear Polypropylene<a href="https://mbpinc.net/product/skirted-96-well-plate-28440-5" title="Skirted 96 Plate, Non-Sterile, Clear Polypropylene" style="color: #000000;"> </a>&ndash; A durable and cost-effective solution for high-volume lab processing.
</li>
</ul>
<h2 data-path-to-node="1"> How to Choose Skirted PCR Plates</h2>
 Well Profile: Low-Profile vs Standard Low-profile 0.1 mL wells are the current default for skirted qPCR plates; the reduced well depth minimises dead volume and supports faster thermal ramp rates on fast-block instruments, including Bio-Rad CFX96 Fast, Applied Biosystems QuantStudio, and Roche LightCycler 480. Standard-profile 0.2 mL wells are used with conventional tall-block cyclers and maximise reaction volume capacity. Colour: Clear, White, or Black Clear skirted plates are the standard for visual inspection and top-read fluorescence systems. White plates increase qPCR sensitivity by up to 2-fold on bottom-read optical configurations by reflecting fluorescence emission back through the detector. Black plates reduce inter-well optical crosstalk in probe-based assays where background fluorescence must be minimised. Two-Component vs One-Component Construction One-component (single polypropylene) skirted plates are cost-effective for general use. Two-component designs combine a polypropylene well array with a polycarbonate skirt to eliminate plate warping at high cycling temperatures, enabling precise robotic positioning even after repeated thermal stress. Barcoding Barcoded skirted plates support LIMS integration and automated sample tracking across multi-step workflows. Barcodes are typically laser-etched (permanent) or adhesive-applied; confirm barcode type before integrating with your lab information management system. Cut Corner Orientation A cut corner at the H1 position (standard) or A12 position aids correct plate orientation in cyclers and liquid handlers. Confirm the required cut corner with your instrument's loading specification. 
<h2 data-path-to-node="1"> Specifications Context</h2>
 Skirted PCR plates conform to ANSI/SBS (also called ANSI/SLAS 1-2004) footprint standards: 127.76 &times; 85.47 mm with 9 mm well spacing. Well walls are uniformly thin (0.25&ndash;0.35 mm) virgin polypropylene; maximum well volume with adhesive film seal is 0.2 mL for standard and 0.1 mL for low-profile formats. Certified DNase-free, RNase-free, human DNA-free, and PCR inhibitor-free per independent lot testing. Thermo Scientific SuperPlate are the leading references for demanding automation applications. Last updated: June 2026.
 If you need help picking the right plate for your system, <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">the MBP team</a> can guide you based on your setup and workflow.

Skirted PCR Plates

DataSheet

NEST PCR Plates

96 well Duo Material PCR plate

402801 by NEST Scientific is a 96-well semi-skirted duo-material PCR plate: USP Class VI polypropylene wells for thermal efficiency paired with a rigid polycarbonate frame for robotic handling. Molded in an ISO 7 cleanroom; lot-tested DNase-free, RNase-free, and DNA-free. Validated for ABI-format instruments.

Manufacturer

0.1ml 96 well Due Material PCR plate, Half-skirted, Clear Frame, Clear Wells, for ABI, 10/pk, 50/cs

0.2ml 96 well Due Material PCR plate, Half-skirted, Clear Frame, Clear Wells, for ABI, 10/pk, 50/cs

0.1ml 96 well Due Material PCR plate, Half-skirted, Detachable, Clear Frame, Clear Wells, for ABI, 10/pk, 50/cs

0.2ml 96 well Due Material PCR plate, Half-skirted, Detachable, Clear Frame, Clear Wells, for ABI, 10/pk, 50/cs

0.1ml 96 well Due Material PCR plate, Half-skirted, Clear Frame, White Wells, for Roche, 10/pk, 50/cs

0.1ml 96 well Due Material PCR plate, Full-skirted, White Frame, Clear Wells, 10/pk, 50/cs

40 ul 384 well Due Material PCR plate, Full-skirted, Clear Frame, Clear Wells, for ABI, 10/pk, 50/cs

40 ul 384 well Due Material PCR plate, Full-skirted, Clear Frame, White Wells, for Roche, 10/pk, 50/cs

40 ul 384 well Due Material PCR plate, Full-skirted, Black Frame, White Wells, for ABI, 10/pk, 50/cs

PCR Plates, Full Skirt

Full skirt PCR Plates

The 402521 by NEST Scientific is a 96-well, 0.1 mL full-skirt PCR plate molded from USP Class VI virgin polypropylene in an ISO 7 cleanroom. H1 notch orientation, sterile, and lot-tested DNase-, RNase-, and human-DNA-free. Clear format compatible with fluorescence detection from above or below; white-well version also available. Compatible with 50+ thermal cycler models.

0.1 mL 96 Well PCR Plate, Full Skirt, Clear, H1 Notch, Sterile, 5/bag, 25/pk, 100/cs

0.1 mL 96 Well PCR Plate, Full Skirt, White, H1 Notch, 5/bag, 25/pk, 100/cs

0.1ml 96 Well PCR Plate, Full Skirt, Clear, H1 notch, 25/pk, 100/cs

40 μl 384Well PCR Plates, full Skirt, Compatible with Roche Machine, White Frame, A24+P24 Notch,White, Sterile,10/pk, 50/cs

40 μl 384Well PCR Plates, full Skirt, Compatible with Roche Machine, White Frame, A24+P24 Notch,White, 10/pk, 50/cs

<h2 dir="ltr">What Are Non-Skirted PCR Plates?</h2>
 Non-skirted PCR plates are 96-well polypropylene thermal cycling plates with no surrounding plastic border. The absence of a skirt gives them the lowest possible profile, making them compatible with the widest range of thermal cyclers &mdash; including older instruments and those with compression lids that leave insufficient clearance for a full or partial skirt. They are the standard choice for manual PCR workflows and labs that prioritise cycler compatibility over automation capability. 
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<h2 dir="ltr">What you will find :<a href="https://mbpinc.net/non-skirted-pcr-plates" title="Non-Skirted PCR Plates"></a></h2>
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<ul data-start="458" data-end="876">
<li style="color: #000000;" data-section-id="zgfdeq" data-start="458" data-end="544">Hard Shell 384-well PCR plates with rigid frame for stability in high-throughput runs</li>
<li style="color: #000000;" data-section-id="rvpzml" data-start="545" data-end="623">Non-skirted 96-well PCR plates, a&nbsp;standard format for routine PCR workflows</li>
<li style="color: #000000;" data-section-id="1ytzau2" data-start="624" data-end="707">Hard Shell 96-well PCR plates with&nbsp;added durability and consistent well alignment</li>
<li style="color: #000000;" data-section-id="pvkgth" data-start="708" data-end="796">Globe Scientific No Skirt PCR Plates, a trusted brand option for reliable performance</li>
<li style="color: #000000;" data-section-id="1teu7sn" data-start="797" data-end="876">PCR Plates, No Skirt general-purpose plates for everyday PCR applications</li>
</ul>
<h2 dir="ltr"> How to Choose Non-Skirted PCR Plates</h2>
 Well Profile: Standard vs Low-Profile Standard-profile 0.2 mL non-skirted plates fit conventional tall-block cyclers, including Bio-Rad T100 and Eppendorf Mastercycler. Low-profile 0.1 mL non-skirted plates are used with fast-block qPCR instruments such as Bio-Rad CFX96 Fast and Applied Biosystems StepOnePlus, where the compression lid requires a low plate profile. Divisible vs One-Piece Plates Divisible (breakable) non-skirted plates are perforated between rows or columns, allowing the plate to be separated into 8-tube strips or smaller sections. This format suits labs running fewer than 96 reactions per plate and reduces plastic waste by avoiding partial-plate discard. One-piece plates are the standard format for full 96-well workflows. Colour: Clear vs White Clear non-skirted plates are standard for end-point PCR and top-read qPCR. White non-skirted plates improve fluorescence sensitivity in bottom-read optical configurations on instruments such as the Applied Biosystems QuantStudio bottom-read modes. Handling Considerations Non-skirted plates are less rigid than skirted formats and can flex during multichannel pipetting if not supported by a plate rack or nest. Use a non-skirted plate rack when dispensing with an 8-channel pipette to prevent well deflection and inconsistent fill volumes. Cap Mats and Sealing Non-skirted plates are sealed with cap mats (silicone or rubber) or adhesive films applied directly to well rims. The absence of a skirt means that heat sealer clamping must be applied carefully to avoid plate deformation. 
<h2 dir="ltr"> Specifications Context</h2>
 Non-skirted plates are moulded from virgin polypropylene with uniformly thin, well walls (0.25&ndash;0.35 mm) for rapid thermal conductivity. The well colour options (clear, white) affect optical performance; clear is the default. Certified DNase-free, RNase-free, human DNA-free, and PCR inhibitor-free per independent lot testing. As of 2026, non-skirted plates remain the highest-volume PCR plate format globally due to broad cycler compatibility and the lowest per-plate cost.&nbsp; 
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<a href="https://mbpinc.net/contact-us">Reach out to the MBP team </a>to request details or pricing for the right fit for your lab.

Non-Skirted PCR Plates

PCR Plates, No Skirt

402101 by NEST Scientific is a 96-well, no-skirt, 0.1 mL polypropylene PCR plate with an H12 notch, available in clear (bottom detector) or white (top detector). Elevated well rims reduce cross-contamination; compatible with Bio-Rad CFX96, ABI QuantStudio, and Eppendorf Mastercycler. Supplied 25/pk, 100/cs.

0.1ml 96 Well PCR Plate, No Skirt, Clear, H12 notch, 25/pk, 100/cs

0.1ml 96 Well PCR Plate, No Skirt, White, H12 notch, 25/pk, 100/cs

0.2ml 96 Well PCR Plate, No Skirt, Elevated wells, Clear, 25/pk, 100/cs

0.2ml 96 Well PCR Plate, No Skirt, White, H1 notch, 25/pk, 100/cs

0.2ml 96 Well PCR Plate, No Skirt, Clear, H1 notch, 25/pk, 100/cs

100ul 96 Well PCR Plates, No Skirt, Roche, A12/H12 Notch, White, 10/pk, 50/cs

PCR Plates, Semi Skirt

NEST Scientific 96-Well Semi Skirt PCR Plates

402301 by NEST Scientific is a 96-well, 0.2 mL semi-skirted PCR plate with standard profile, A12 notch, and optically clear construction. Compatible with bottom-read fluorescence thermal cyclers and semi-skirt robotic grippers. Supplied 25 plates per pack, 100 plates per case. Clear alpha-numeric labeling for positive plate orientation on automated decks.

0.1ml 96 Well PCR Plate, Semi Skirt, Clear, A1 notch, 25/pk, 100/cs, for ABI machine (ABI Equivalent N801-0560)

0.1ml 96 Well PCR Plate, Semi Skirt, White, A1 notch, 25/pk, 100/cs

0.2ml 96 Well PCR Plate, Semi Skirt, Clear, A12 notch, 25/pk, 100/cs

0.2ml 96 Well PCR Plate, Semi Skirt, Clear, A12 notch, 25/pk, 100/cs, for ABI machine (ABI Equivalent ?????)

100 μl 96 Well PCR Plates,Semi Skirt,Roche,H12 Notch,White, 10/pk, 50/cs

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