Name: NEST Scientific 96-Well Semi Skirt PCR Plates
SKU: 402301
Availability: InStock

PCR Plates

<h2 data-path-to-node="3">What Are PCR Plates?</h2>
 PCR plates are single-use, thin-wall polypropylene multi-well vessels designed for the thermal cycling demands of PCR, qPCR, RT-PCR, and genotyping. The 96-well format (12 columns &times; 8 rows) is the standard for most research and clinical labs; 384-well plates serve ultra-high-throughput applications. Well volumes are 0.2 mL (standard profile) for conventional block cyclers or 0.1 mL (low profile) for fast-block instruments. The skirt &mdash; the rigid plastic border surrounding the well array &mdash; determines automation compatibility, cycler lid clearance, and plate rigidity.&nbsp;
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li data-section-id="1xmdc3x" data-start="0" data-end="87"><a href="https://mbpinc.net/consumables/pcr-consumables/pcr-plates/semi-skirted-plates">Semi-skirted PCR plates: </a>for a balance of plate rigidity and instrument compatibility.</li>
<li data-section-id="y5pq2h" data-start="89" data-end="156"><a href="https://mbpinc.net/consumables/pcr-consumables/pcr-plates/hard-shell-pcr-plates">Hard shell PCR plates: </a>designed with a reinforced plate structure.</li>
<li data-section-id="8t808z" data-start="158" data-end="243"><a href="https://mbpinc.net/consumables/pcr-consumables/pcr-plates/skirted-pcr-plates">Skirted PCR plates:</a> featuring a full peripheral skirt for enhanced handling support.</li>
<li data-section-id="1d0aj4c" data-start="245" data-end="343" data-is-last-node=""><a href="https://mbpinc.net/consumables/pcr-consumables/pcr-plates/non-skirted-pcr-plates">Non-skirted PCR plates:</a> with a compact design for applications requiring minimal plate footprint.</li>
</ul>
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<h2 data-path-to-node="3">How to Choose PCR Plates</h2>
 Skirt Format Fully-skirted plates have a full-height ANSI/SBS-compliant border (127.76 &times; 85.47 mm footprint) and are required for robotic liquid handlers (Hamilton STAR, Tecan EVO, PerkinElmer JANUS) and automated stackers. Semi-skirted plates have a partial skirt providing enough rigidity for multichannel pipetting while maintaining thermal cycler block compatibility across a wider range of instruments. Non-skirted plates have no border and fit virtually any cycler block, including instruments whose compression lids would be obstructed by a full skirt. Well Profile: Standard vs Low-Profile Standard-profile 0.2 mL wells are used with conventional (tall) block cyclers. Low-profile 0.1 mL wells are required for fast-block instruments, including the Applied Biosystems QuantStudio, Bio-Rad CFX96 Fast, and Roche LightCycler 480, where the reduced well depth shortens the thermal path to the sample. Colour: Clear, White, or Black Clear plates allow visual inspection of well volumes and are the default for end-point PCR. White plates increase qPCR sensitivity in bottom-read optical systems by reflecting fluorescence emission back through the detector. Black plates reduce background fluorescence in probe-based assays where cross-well optical interference is a concern. Sealing Compatibility Skirted and semi-skirted plates seal reliably with adhesive films and heat sealers; the rigid skirt supports even film application and reduces edge-well evaporation. Non-skirted plates require cap mats or adhesive seals that adhere directly to the well rim. 
<h2 data-path-to-node="3"> Specifications Context</h2>
 PCR plates are moulded from virgin, low-extractable medical-grade polypropylene; well walls are uniformly thin (typically 0.25&ndash;0.35 mm) to maximise thermal conductivity. ANSI/SBS footprint standard (127.76 &times; 85.47 mm) applies to skirted plates for instrument interoperability. Plates are certified DNase-free, RNase-free, human DNA-free, and PCR inhibitor-free per lot. Cut corners (typically at H1/A12 position) and alpha-numeric well labelling aid plate orientation.Thermo Fisher SuperPlate skirted formats are the leading references for robotic applications; semi-skirted plates from Sorenson and equivalent lines serve the broad research market.&nbsp; 
<a href="https://mbpinc.net/contact-us">Connect with the MBP team </a>today to request a quote and find the perfect PCR plate configuration for your lab's specific needs.

<h2>What Are Semi-Skirted PCR Plates?</h2>
 Semi-skirted PCR plates have a partial plastic border extending approximately half the height of a fully skirted plate, surrounding part of the well array. This partial skirt adds enough structural rigidity to support multichannel pipetting. It provides a surface for labelling, while keeping the overall plate height low enough for compatibility with most thermal cycler compression lids that a full skirt would obstruct. Semi-skirted plates represent the most common format in routine qPCR and genotyping labs, where fully automated robotic handling is not required. Semi-skirted is the default choice for most routine PCR and qPCR labs that mix manual pipetting with occasional automated steps. 
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<h2 data-start="476" data-end="501">What you will find: </h2>
<ul data-start="502" data-end="1108">
<li style="color: #000000;" data-section-id="47u9b8" data-start="502" data-end="589">Hard shell 384-well PCR plates &ndash; enhanced rigidity for high-throughput applications</li>
<li style="color: #000000;" data-section-id="1oohzes" data-start="590" data-end="670">Semi-skirted, 96-well PCR plates &ndash; balanced design for routine PCR workflows</li>
<li style="color: #000000;" data-section-id="1n8sxu4" data-start="671" data-end="748">Hard Shell 96-well PCR plates &ndash; improved structural support and alignment</li>
<li style="color: #000000;" data-section-id="1gxz5rn" data-start="749" data-end="830">Globe Scientific Half Skirt PCR Plates &ndash; reliable option from a trusted brand</li>
<li style="color: #000000;" data-section-id="wcryl4" data-start="831" data-end="897">PCR Plates, Semi Skirt &ndash; versatile plates for standard lab use</li>
<li style="color: #000000;" data-section-id="dlek6q" data-start="898" data-end="985">96-well Duo Material PCR plate &ndash; optimized materials for performance and durability</li>
<li style="color: #000000;" data-section-id="4vb9o1" data-start="986" data-end="1108">Semi-Skirt 96 Plate, Clear Polypropylene, Non-Sterile &ndash; clear construction for visibility and general applications</li>
</ul>
<h2> How to Choose Semi-Skirted PCR Plates</h2>
 Well Profile and Volume Low-profile 0.1 mL semi-skirted plates are the standard for qPCR on fast-block instruments, including Bio-Rad CFX96, Applied Biosystems StepOnePlus, and Roche LightCycler 480. Standard-profile 0.2 mL wells work with conventional block cyclers and accommodate larger reaction volumes for cloning or high-concentration template workflows. Colour: Clear vs White Clear semi-skirted plates suit end-point PCR and top-read qPCR systems. White semi-skirted plates increase fluorescence signal in bottom-read qPCR optical configurations. Thermo Fisher offers white semi-skirted formats for this application. Cut Corner for Orientation Semi-skirted plates typically feature a notched or cut corner at the A12 position for plate orientation &mdash; a feature originating in sequencing plate formats and now standard across most qPCR-compatible semi-skirted plates. Confirm the cut corner position against your instrument's plate alignment specification. Sealing The partial skirt of semi-skirted plates supports adhesive film sealing and is compatible with most heat sealers. The skirt also reduces edge-well evaporation compared to non-skirted plates, improving Ct uniformity across plate positions. Robotic Compatibility Semi-skirted plates provide enough grip surface for some robotic arm designs and are listed as compatible with select liquid handlers; however, for demanding robotic workflows with gripper systems or conveyor belts, fully skirted ANSI/SBS-compliant plates remain the correct specification. 
<h2> Specifications Context</h2>
 Semi-skirted 96-well plates are moulded from virgin polypropylene with uniformly thin walls (0.25&ndash;0.35 mm) for consistent thermal conductivity. The partial skirt height is approximately 50% of a full ANSI/SBS skirt height, placing the plate between non-skirted and fully skirted in both rigidity and cycler compatibility. All plates are independently certified DNase-free, RNase-free, human DNA-free, and PCR inhibitor-free per lot.&nbsp; 
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Let <a href="https://mbpinc.net/contact-us">the MBP team&nbsp;</a>help you choose the right plate format to match your workflow and instrumentation.

Semi-Skirted Plates

PCR Plates, Semi Skirt

NEST Scientific 96-Well Semi Skirt PCR Plates

NEST Scientific semi-skirted 96-well PCR plates in five variants: 0.2 mL Clear A12 (402301), 0.2 mL Clear A12 ABI (402601), 0.1 mL Clear A1 ABI equiv. N801-0560 (402401), 0.1 mL White A1 (402411), 0.1 mL White Roche H12 (402712). Standard profile, polypropylene, 25/pk or 10/pk.

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