Name: 96 well Duo Material PCR plate
SKU: 402801
Availability: InStock

PCR Plates

<h2 data-path-to-node="3">What Are PCR Plates?</h2>
 PCR plates are single-use, thin-wall polypropylene multi-well vessels designed for the thermal cycling demands of PCR, qPCR, RT-PCR, and genotyping. The 96-well format (12 columns &times; 8 rows) is the standard for most research and clinical labs; 384-well plates serve ultra-high-throughput applications. Well volumes are 0.2 mL (standard profile) for conventional block cyclers or 0.1 mL (low profile) for fast-block instruments. The skirt &mdash; the rigid plastic border surrounding the well array &mdash; determines automation compatibility, cycler lid clearance, and plate rigidity.&nbsp;
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<h2 dir="ltr">What you will find:</h2>
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<li data-section-id="1xmdc3x" data-start="0" data-end="87"><a href="https://mbpinc.net/consumables/pcr-consumables/pcr-plates/semi-skirted-plates">Semi-skirted PCR plates: </a>for a balance of plate rigidity and instrument compatibility.</li>
<li data-section-id="y5pq2h" data-start="89" data-end="156"><a href="https://mbpinc.net/consumables/pcr-consumables/pcr-plates/hard-shell-pcr-plates">Hard shell PCR plates: </a>designed with a reinforced plate structure.</li>
<li data-section-id="8t808z" data-start="158" data-end="243"><a href="https://mbpinc.net/consumables/pcr-consumables/pcr-plates/skirted-pcr-plates">Skirted PCR plates:</a> featuring a full peripheral skirt for enhanced handling support.</li>
<li data-section-id="1d0aj4c" data-start="245" data-end="343" data-is-last-node=""><a href="https://mbpinc.net/consumables/pcr-consumables/pcr-plates/non-skirted-pcr-plates">Non-skirted PCR plates:</a> with a compact design for applications requiring minimal plate footprint.</li>
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<h2 data-path-to-node="3">How to Choose PCR Plates</h2>
 Skirt Format Fully-skirted plates have a full-height ANSI/SBS-compliant border (127.76 &times; 85.47 mm footprint) and are required for robotic liquid handlers (Hamilton STAR, Tecan EVO, PerkinElmer JANUS) and automated stackers. Semi-skirted plates have a partial skirt providing enough rigidity for multichannel pipetting while maintaining thermal cycler block compatibility across a wider range of instruments. Non-skirted plates have no border and fit virtually any cycler block, including instruments whose compression lids would be obstructed by a full skirt. Well Profile: Standard vs Low-Profile Standard-profile 0.2 mL wells are used with conventional (tall) block cyclers. Low-profile 0.1 mL wells are required for fast-block instruments, including the Applied Biosystems QuantStudio, Bio-Rad CFX96 Fast, and Roche LightCycler 480, where the reduced well depth shortens the thermal path to the sample. Colour: Clear, White, or Black Clear plates allow visual inspection of well volumes and are the default for end-point PCR. White plates increase qPCR sensitivity in bottom-read optical systems by reflecting fluorescence emission back through the detector. Black plates reduce background fluorescence in probe-based assays where cross-well optical interference is a concern. Sealing Compatibility Skirted and semi-skirted plates seal reliably with adhesive films and heat sealers; the rigid skirt supports even film application and reduces edge-well evaporation. Non-skirted plates require cap mats or adhesive seals that adhere directly to the well rim. 
<h2 data-path-to-node="3"> Specifications Context</h2>
 PCR plates are moulded from virgin, low-extractable medical-grade polypropylene; well walls are uniformly thin (typically 0.25&ndash;0.35 mm) to maximise thermal conductivity. ANSI/SBS footprint standard (127.76 &times; 85.47 mm) applies to skirted plates for instrument interoperability. Plates are certified DNase-free, RNase-free, human DNA-free, and PCR inhibitor-free per lot. Cut corners (typically at H1/A12 position) and alpha-numeric well labelling aid plate orientation.Thermo Fisher SuperPlate skirted formats are the leading references for robotic applications; semi-skirted plates from Sorenson and equivalent lines serve the broad research market.&nbsp; 
<a href="https://mbpinc.net/contact-us">Connect with the MBP team </a>today to request a quote and find the perfect PCR plate configuration for your lab's specific needs.

<h2>What Are Semi-Skirted PCR Plates?</h2>
 Semi-skirted PCR plates have a partial plastic border extending approximately half the height of a fully skirted plate, surrounding part of the well array. This partial skirt adds enough structural rigidity to support multichannel pipetting. It provides a surface for labelling, while keeping the overall plate height low enough for compatibility with most thermal cycler compression lids that a full skirt would obstruct. Semi-skirted plates represent the most common format in routine qPCR and genotyping labs, where fully automated robotic handling is not required. Semi-skirted is the default choice for most routine PCR and qPCR labs that mix manual pipetting with occasional automated steps. 
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<h2 data-start="476" data-end="501">What you will find: </h2>
<ul data-start="502" data-end="1108">
<li style="color: #000000;" data-section-id="47u9b8" data-start="502" data-end="589">Hard shell 384-well PCR plates &ndash; enhanced rigidity for high-throughput applications</li>
<li style="color: #000000;" data-section-id="1oohzes" data-start="590" data-end="670">Semi-skirted, 96-well PCR plates &ndash; balanced design for routine PCR workflows</li>
<li style="color: #000000;" data-section-id="1n8sxu4" data-start="671" data-end="748">Hard Shell 96-well PCR plates &ndash; improved structural support and alignment</li>
<li style="color: #000000;" data-section-id="1gxz5rn" data-start="749" data-end="830">Globe Scientific Half Skirt PCR Plates &ndash; reliable option from a trusted brand</li>
<li style="color: #000000;" data-section-id="wcryl4" data-start="831" data-end="897">PCR Plates, Semi Skirt &ndash; versatile plates for standard lab use</li>
<li style="color: #000000;" data-section-id="dlek6q" data-start="898" data-end="985">96-well Duo Material PCR plate &ndash; optimized materials for performance and durability</li>
<li style="color: #000000;" data-section-id="4vb9o1" data-start="986" data-end="1108">Semi-Skirt 96 Plate, Clear Polypropylene, Non-Sterile &ndash; clear construction for visibility and general applications</li>
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<h2> How to Choose Semi-Skirted PCR Plates</h2>
 Well Profile and Volume Low-profile 0.1 mL semi-skirted plates are the standard for qPCR on fast-block instruments, including Bio-Rad CFX96, Applied Biosystems StepOnePlus, and Roche LightCycler 480. Standard-profile 0.2 mL wells work with conventional block cyclers and accommodate larger reaction volumes for cloning or high-concentration template workflows. Colour: Clear vs White Clear semi-skirted plates suit end-point PCR and top-read qPCR systems. White semi-skirted plates increase fluorescence signal in bottom-read qPCR optical configurations. Thermo Fisher offers white semi-skirted formats for this application. Cut Corner for Orientation Semi-skirted plates typically feature a notched or cut corner at the A12 position for plate orientation &mdash; a feature originating in sequencing plate formats and now standard across most qPCR-compatible semi-skirted plates. Confirm the cut corner position against your instrument's plate alignment specification. Sealing The partial skirt of semi-skirted plates supports adhesive film sealing and is compatible with most heat sealers. The skirt also reduces edge-well evaporation compared to non-skirted plates, improving Ct uniformity across plate positions. Robotic Compatibility Semi-skirted plates provide enough grip surface for some robotic arm designs and are listed as compatible with select liquid handlers; however, for demanding robotic workflows with gripper systems or conveyor belts, fully skirted ANSI/SBS-compliant plates remain the correct specification. 
<h2> Specifications Context</h2>
 Semi-skirted 96-well plates are moulded from virgin polypropylene with uniformly thin walls (0.25&ndash;0.35 mm) for consistent thermal conductivity. The partial skirt height is approximately 50% of a full ANSI/SBS skirt height, placing the plate between non-skirted and fully skirted in both rigidity and cycler compatibility. All plates are independently certified DNase-free, RNase-free, human DNA-free, and PCR inhibitor-free per lot.&nbsp; 
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Let <a href="https://mbpinc.net/contact-us">the MBP team&nbsp;</a>help you choose the right plate format to match your workflow and instrumentation.

Semi-Skirted Plates

<h2 data-path-to-node="1"> What Are Skirted PCR Plates?</h2>
 Skirted PCR plates are 96-well polypropylene thermal cycling plates with a full-height plastic border &mdash; the skirt &mdash; surrounding the well array. The skirt raises the overall plate height to match the ANSI/SBS microplate footprint standard (127.76 &times; 85.47 mm external dimensions), creating a uniform grip surface that robotic arms, plate grippers, and automated stackers can handle reliably. They are the required format for liquid-handling automation platforms and high-throughput PCR workflows where consistent plate positioning is critical. Choose fully skirted plates when your workflow involves robotic liquid handling, automated stackers, or plate readers requiring SBS-compliant positioning.
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<h2 data-path-to-node="4">What You Will Find: </h2>
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Hard Shell 384-well PCR Plates<a href="https://www.google.com/search?q=https://mbpinc.net/product-category/consumables/pcr-consumables/pcr-plates/hard-shell-pcr-plates/" style="color: #000000;"> </a>&ndash; The industry standard for high-throughput screening and automated liquid handling.
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Hard Shell 96-well PCR Plates &ndash; Combining rigidity with a 96-well format for routine yet high-precision workflows.
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Globe Scientific Full Skirt PCR Plates &ndash; Reliable, automation-friendly plates designed for consistent thermal cycling.
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<li style="color: #000000;">
Full Skirt PCR Plates<a _ngcontent-ng-c3793280901="" target="_blank" rel="noopener" externallink="" _nghost-ng-c3773094726="" jslog="197247;track:generic_click,impression,attention;BardVeMetadataKey:[[&quot;r_98f8600e63dbeb30&quot;,&quot;c_c4e8a23c27447c36&quot;,null,&quot;rc_fa92ce727d8e45fc&quot;,null,null,&quot;en&quot;,null,1,null,null,1,0]]" href="https://www.google.com/search?q=https://mbpinc.net/product-category/consumables/pcr-consumables/pcr-plates/skirted-pcr-plates/" class="ng-star-inserted" data-hveid="0" decode-data-ved="1" data-ved="0CAAQ_4QMahgKEwjZx-625v-TAxUAAAAAHQAAAAAQsAQ" style="color: #000000;"></a> &ndash; Features a full perimeter skirt for superior stability and easy gripping during automated handling.
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96-well Duo Material PCR Plate&ndash; Engineered with dual-material technology to eliminate plate warping and bowing.
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Skirted 96 Plate, Non-Sterile, Clear Polypropylene<a href="https://mbpinc.net/product/skirted-96-well-plate-28440-5" title="Skirted 96 Plate, Non-Sterile, Clear Polypropylene" style="color: #000000;"> </a>&ndash; A durable and cost-effective solution for high-volume lab processing.
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</ul>
<h2 data-path-to-node="1"> How to Choose Skirted PCR Plates</h2>
 Well Profile: Low-Profile vs Standard Low-profile 0.1 mL wells are the current default for skirted qPCR plates; the reduced well depth minimises dead volume and supports faster thermal ramp rates on fast-block instruments, including Bio-Rad CFX96 Fast, Applied Biosystems QuantStudio, and Roche LightCycler 480. Standard-profile 0.2 mL wells are used with conventional tall-block cyclers and maximise reaction volume capacity. Colour: Clear, White, or Black Clear skirted plates are the standard for visual inspection and top-read fluorescence systems. White plates increase qPCR sensitivity by up to 2-fold on bottom-read optical configurations by reflecting fluorescence emission back through the detector. Black plates reduce inter-well optical crosstalk in probe-based assays where background fluorescence must be minimised. Two-Component vs One-Component Construction One-component (single polypropylene) skirted plates are cost-effective for general use. Two-component designs combine a polypropylene well array with a polycarbonate skirt to eliminate plate warping at high cycling temperatures, enabling precise robotic positioning even after repeated thermal stress. Barcoding Barcoded skirted plates support LIMS integration and automated sample tracking across multi-step workflows. Barcodes are typically laser-etched (permanent) or adhesive-applied; confirm barcode type before integrating with your lab information management system. Cut Corner Orientation A cut corner at the H1 position (standard) or A12 position aids correct plate orientation in cyclers and liquid handlers. Confirm the required cut corner with your instrument's loading specification. 
<h2 data-path-to-node="1"> Specifications Context</h2>
 Skirted PCR plates conform to ANSI/SBS (also called ANSI/SLAS 1-2004) footprint standards: 127.76 &times; 85.47 mm with 9 mm well spacing. Well walls are uniformly thin (0.25&ndash;0.35 mm) virgin polypropylene; maximum well volume with adhesive film seal is 0.2 mL for standard and 0.1 mL for low-profile formats. Certified DNase-free, RNase-free, human DNA-free, and PCR inhibitor-free per independent lot testing. Thermo Scientific SuperPlate are the leading references for demanding automation applications. Last updated: June 2026.
 If you need help picking the right plate for your system, <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">the MBP team</a> can guide you based on your setup and workflow.

Skirted PCR Plates

DataSheet

NEST PCR Plates

96 well Duo Material PCR plate

402801 by NEST Scientific is a 96-well semi-skirted duo-material PCR plate: USP Class VI polypropylene wells for thermal efficiency paired with a rigid polycarbonate frame for robotic handling. Molded in an ISO 7 cleanroom; lot-tested DNase-free, RNase-free, and DNA-free. Validated for ABI-format instruments.

Manufacturer

96 well Duo Material PCR plate

SKU : 402801