Cell Culture

Erlenmeyer Flasks Accessories

<h2 dir="ltr">&nbsp;What are Erlenmeyer Flask Accessories?</h2>
 Erlenmeyer flask accessories are the replacement caps, transfer cap assemblies, vent filters, and adapter fittings used alongside PC and PETG shake flasks to customize gas exchange, enable closed-system liquid transfer, and replace consumable caps between culture runs. Accessory selection determines whether a shake flask acts as a standard culture vessel, a sealed storage container, or a closed-system liquid transfer assembly in a cGMP workflow.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li>Accessories compatible with high-efficiency Erlenmeyer flasks in 2 L, 3 L, and 5 L formats.</li>
<li>Available in bi-directional, inverted, and multifunctional transfer cap configurations.</li>
<li>Supports both standard-neck and wide-mouth 3 L flask designs.</li>
<li>Includes options with integrated silicon tubing for transfer applications.</li>
<li>Enables customization of flask workflows through interchangeable transfer cap accessories.</li>
</ul>
<h2 dir="ltr">&nbsp;</h2>
<h2 dir="ltr">How to Choose Erlenmeyer Flask Accessories</h2>
 Vented Caps: Standard vs. Oversized Membrane Standard vented Erlenmeyer caps have a 0.22 &micro;m PTFE membrane in a small-diameter HDPE housing. NEST Scientific vented caps feature an oversized membrane area that increases the gas exchange surface, supporting higher oxygen mass transfer for dense suspension cultures at elevated shaker speeds. At 150 RPM with 20&ndash;25% fill, NEST oversized vented caps provide sufficient oxygen transfer for mammalian suspension cultures at densities up to approximately 5 &times; 10⁶ cells/mL. For cultures targeting densities above 10 &times; 10⁶ cells/mL in a standard shake flask format, a sparged bioreactor system is more appropriate. Plug Seal Caps: Autoclave vs. Non-Autoclave HDPE plug seal caps for NEST PC Erlenmeyer flasks are compatible with autoclave cycles at 121 &deg;C when loosened 1&ndash;1.5 turns before autoclaving to prevent steam pressure buildup. Plug seal caps for NEST PETG flasks are compatible with static incubation and transport but not autoclaving (PETG maximum temperature: +60 &deg;C). Confirm whether your accessory cap is rated for autoclave use before including it in a steam sterilization cycle. Transfer Cap Assemblies for Closed-System Culture NEST customized transfer cap assemblies combine an Erlenmeyer-compatible cap body with a bi-directional transfer port, TPE tubing, and a Luer lock or CPC connector &mdash; enabling aseptic inoculation, sampling, or media addition to a running shake flask culture without opening the flask. This closed-system capability is used in cGMP process development, GMP seed train management, and contamination-critical research workflows. Transfer caps are available in sizes matching NEST 125 mL, 250 mL, 500 mL, 1,000 mL, 2,000 mL, and 3,000 mL flask neck formats. Cap Sizing and Flask Compatibility Erlenmeyer flask caps are sized to the neck thread diameter of the flask. NEST Scientific PC and PETG Erlenmeyer flasks use proprietary screw-thread neck dimensions &mdash; confirm the specific cap catalog number matches your flask's neck size before ordering replacement caps. Do not mix cap brands across flask brands without verifying thread compatibility; mismatched caps create gas leaks at the vent membrane, contaminating cultures. Replacement Cap Frequency for Reusable PC Flasks For reusable PC flasks, replace the vented cap after each culture run to prevent membrane clogging from media splatter, condensation, and biofilm formation on the PTFE membrane. Reusing a contaminated or clogged vent membrane reduces gas exchange efficiency, creating a DO deficit that depresses cell growth without a visible contamination event. NEST vented caps for PC flasks are available in bulk packs for cost-efficient per-run replacement. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific Erlenmeyer flask accessories &mdash; including replacement vented caps, plug seal caps, and transfer cap assemblies &mdash; are manufactured from USP Class VI HDPE (cap housing) and USP Class VI PTFE (0.22 &micro;m membrane), with TPE tubing in transfer assemblies. All accessory caps are sterile and non-pyrogenic per the lot certificate. The 0.22 &micro;m membrane pore size is the standard for microbial retention in cell culture venting applications. Transfer cap assemblies compatible with the NEST Erlenmeyer flask line are available in sizes for 125 mL through 3,000 mL flasks, individually wrapped in sterile packaging. NEST Scientific is ISO 9001:2015 and ISO 13485 certified.&nbsp; 
Power your scientific breakthroughs with high-precision labware.&nbsp;<a href="https://mbpinc.net/contact-us" title="the MBP team" style="color: #236fa1;">Contact the MBP team</a> today for bulk availability, technical specifications, and expert support.

Erlenmeyer Flask Accessories

DataSheet

5L High Efficiency Erlenmeyer Flask Inverted Transfer Cap

The C10431-AZC090B by NEST Scientific is an inverted transfer cap for the 5 L NEST High Efficiency Erlenmeyer Flask, enabling closed-system liquid transfer that minimizes external contamination risk during media handling and bioprocess operations. Features a 90 cm transfer tube, 0.22 µm vent filter, and ships sterile to SAL 10⁻⁶. Packed 1 per pack, 4 per case.

Manufacturer

5L High Efficiency Erlenmeyer Flask Inverted Transfer Cap with TPE Tube (90cm 1/8" ID 1/4" OD), Vent Filter (0.22 μm Φ42mm), without Flask, Sterile, 1/pk, 4/cs

5L High Efficiency Erlenmeyer Flask Inverted Transfer Cap with TPE Tube ( 90cm 1/4" ID 7/16" OD), Vent Filter (0.22 μm Φ42mm), without Flask, Sterile, 1/pk, 4/cs

5L High Efficiency Erlenmeyer Flask Multifunctional Transfer Cap

C10411-AJE092B by NEST Scientific is a sterile multifunctional transfer cap for 5 L High Efficiency Erlenmeyer Flasks, with 92 cm weldable TPE tubing (1/8" ID × 1/4" OD), sampling nozzle with one-way valve, and a 0.22 µm vent filter (Φ50 mm). Enables closed-system liquid transfer and aseptic sampling. SAL 10⁻⁶, endotoxin-free, animal-origin-free; 1/pk, 4/cs.

5L High Efficiency Erlenmeyer Flask Multifunctional Transfer Cap with TPE Tube (92cm 1/8" ID 1/4" OD) and a Sampling Nozzle, Vent Filter (0.22 μm Φ50mm), without Flask, Sterile, 1/pk, 4/cs

5L High Efficiency Erlenmeyer Flask Multifunctional Transfer Cap with TPE Tube ( 90cm 1/4" ID 7/16" OD), Vent Filter (0.22 μm Φ24mm), without Flask, Sterile, 1/pk, 4/cs

5L High Efficiency Erlenmeyer Flask Multifunctional Transfer Cap with Silicon Tube (100cm 3/16" ID 3/8" OD), Male CPC Connector with Female Sealing Cap, Vent Filter (0.22 μm Φ24mm), without Flask, Sterile, 1/pk, 4/cs

Cell Culture Inserts

<h2 dir="ltr">What are Cell Culture Inserts?</h2>
 Cell culture inserts (also called Transwell inserts or permeable supports) are sterile, single-use polystyrene well inserts with a permeable membrane floor that suspend cells above the bottom of a standard multi-well plate, creating separate apical (upper, inside insert) and basolateral (lower, outside insert in the well below) compartments. The membrane allows diffusion of media, nutrients, metabolites, and signaling molecules between the two compartments while physically separating the two cell populations.&nbsp;Choose PET membranes for imaging and clear-bottom visualization; choose PC membranes for superior cell attachment; choose pore size based on whether cells need to migrate through the membrane (&ge;3 &micro;m) or be retained on it (0.4 &micro;m and 1 &micro;m).
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="color: #000000;" role="presentation">6 Inserts / 6-Well Plate for Tissue Engineering - Ideal for skin models and complex cell differentiation where a larger surface area is required.</li>
<li dir="ltr" style="color: #000000;" role="presentation">12 Inserts / 12-Well Plate for Transport Assays - Balanced format for nutrient diffusion studies and metabolic monitoring.</li>
<li dir="ltr" style="color: #000000;" role="presentation">12 Inserts / 24-Well Plate for&nbsp;High Throughput Screening - Designed for migration and chemotaxis assays with efficient reagent use.</li>
<li style="color: #000000;">SPLInsert&trade; Hanging formats with transparent, sterile PC or PET membranes.</li>
<li style="color: #000000;">SPLInsert&trade; Standing format for cell culture insert applications.</li>
<li style="color: #000000;">100 mm cell culture insert dishes with 3.0 &mu;m PC membranes, translucent construction, tissue-culture treatment, and sterile packaging.</li>
</ul>
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<h2 dir="ltr">How to Choose Cell Culture Inserts</h2>
 PET vs. PC Membrane PET (polyethylene terephthalate) membranes are optically transparent, allowing direct visualization of cells growing on the membrane by standard phase-contrast and fluorescence microscopy without removing the insert. PET is the first choice when imaging cells on the membrane is part of the experimental readout (tight-junction immunofluorescence, GFP localization, wound-healing on the membrane). PC (polycarbonate) membranes provide superior cell attachment due to their higher surface energy &mdash; often used for primary epithelial, endothelial, and intestinal cells that are difficult to culture on PET. PC membranes are opaque, blocking transmitted light microscopy through the membrane. Pore Size by Application 0.4 &micro;m pore inserts prevent cell migration and cell-cell contact between apical and basolateral compartments while allowing free diffusion of molecules &lt;0.4 &micro;m (nutrients, cytokines, small molecules, metabolites). Standard for epithelial barrier function assays (TEER measurement, permeability assays), secretion studies (measuring cytokine secretion from the basolateral side), and any co-culture where cell-cell contact must be prevented. 1 &micro;m pore inserts similarly prevent cell migration but allow slightly larger molecule diffusion &mdash; used for exosome transport studies. 3 &micro;m pore inserts allow poorly migratory cells (some endothelial, fibroblast lines) to migrate through. 8 &micro;m pore inserts support tumor cell migration, invasion, chemotaxis, and hematotaxis assays &mdash; the standard pore size for Boyden chamber&ndash;style migration and invasion assays. Plate Format and Insert Configuration NEST Scientific inserts are pre-packed in multi-well plates: 6-well plate format (6 inserts per plate), 12-well plate format (12 inserts per plate), 24-well plate format (12 inserts per plate). Inserts are centered in wells by their flange geometry, preventing the membrane from touching the well sides and creating a wicking path. The apical-basolateral gap (distance from membrane floor to well bottom) is designed to allow media access to the basolateral face and pipette access to the lower chamber for sampling. TC-Treated vs. Non-Treated Membrane TC-treated membrane inserts promote adherent mammalian cell monolayer formation on the insert membrane. Non-treated membrane inserts (some PC formats) are used for migration and invasion assays where cells in the apical chamber must actively migrate through the membrane pore to reach the basolateral side &mdash; a non-treated surface reduces passive cell adhesion that would produce false-positive migration signals. Transferability NEST Scientific cell culture inserts can be transferred from one plate to a fresh plate for media changes, cumulative secretion studies, and time-course experiments &mdash; a key advantage over the non-insert well format. Transfer the insert using sterile forceps or the insert flange. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific cell culture inserts are manufactured from polystyrene with PET or PC membranes in TC-treated (vacuum plasma, contact angle &lt;10&deg;) or non-treated formats. Inserts are packed in individually sealed, peelable Tyvek film and blister packaging for sterility assurance &mdash; one plate per pack, sterile. E-beam sterilized to SAL 10⁻⁶; DNase/RNase-free and non-pyrogenic per lot. The insert flange geometry is designed to center the insert in the well, preventing membrane-wall contact and wicking. NEST Scientific cell culture inserts in PET and PC membranes across the four pore sizes (0.4, 1, 3, 8 &micro;m) and three plate formats (6-, 12-, 24-well) are available from MBP. 
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Datsheet

Cell Culture Insert Specifications

6 Cell Culture Inserts+6 Well Plate Specifications

6 Cell Culture Inserts+6 Well Plate, PC Membrane

723101 by NEST Scientific is a kit of 6 polycarbonate (PC) cell culture inserts pre-seated in a 6-well receiver plate, with a 0.4 µm pore size, TC-treated transparent membrane, and sterile packaging in peelable Tyvek blister packs. The 0.4 µm pore blocks cell transmigration while permitting small-molecule transport for TEER, barrier function, and co-culture studies. Ships 6 inserts per kit, 24 kits per case.

6 Cell Culture Inserts+6 Well Plate, 0.4 μm, PC Membrane, Opaque, TC, Sterile, 6/pk, 24/cs

6 Cell Culture Inserts+6 Well Plate, 3.0 μm, PC Membrane, Translucent, TC, Sterile, 6/pk, 24/cs

6 Cell Culture Inserts+6 Well Plate, 8.0 μm, PC Membrane, Translucent, TC, Sterile, 6/pk, 24/cs

6 Cell Culture Inserts+6 Well Plate, 0.4 μm, PC Membrane, Opaque, Non-Treated, Sterile, 6/pk, 24/cs

6 Cell Culture Inserts+6 Well Plate, 3.0 μm, PC Membrane, Translucent, Non-Treated, Sterile, 6/pk, 24/cs

6 Cell Culture Inserts+6 Well Plate, 8.0 μm, PC Membrane, Translucent, Non-Treated, Sterile, 6/pk, 24/cs

6 Cell Culture Inserts+6 Well Plate, PET Membrane

NEST Scientific 723121: 6 hanging cell culture inserts with 0.4 µm TC-treated transparent PET membrane, pre-loaded in a 6-well companion plate. Sterile. PET transparency enables direct fluorescence imaging through the membrane. Order at <a href="https://www.mbpinc.net/quickorder">mbpinc.net/quickorder</a>.

6 Cell Culture Inserts+6 Well Plate, 0.4 μm, PET Membrane, Opaque, TC, Sterile, 6/pk, 24/cs

6 Cell Culture Inserts+6 Well Plate, 1.0 μm, PET Membrane, Opaque, TC, Sterile, 6/pk, 24/cs

6 Cell Culture Inserts+6 Well Plate, 3.0 μm, PET Membrane, Translucent, TC, Sterile, 6/pk, 24/cs

6 Cell Culture Inserts+6 Well Plate, 8.0 μm, PET Membrane, Transparent, TC, Sterile, 6/pk, 24/cs

6 Cell Culture Inserts+6 Well Plate, 0.4 μm, PET Membrane, Opaque, Non-Treated, Sterile, 6/pk, 24/cs

6 Cell Culture Inserts+6 Well Plate, 1.0 μm, PET Membrane, Opaque, Non-Treated, Sterile, 6/pk, 24/cs

6 Cell Culture Inserts+6 Well Plate, 3.0 μm, PET Membrane, Translucent, Non-Treated, Sterile, 6/pk, 24/cs

6 Cell Culture Inserts+6 Well Plate, 8.0 μm, PET Membrane, Transparent, Non-Treated, Sterile, 6/pk, 24/cs

Cell Culture Plates

<h2 class="p2"> What are Cell Culture Plates?</h2>
 Cell culture plates are sterile, single-use polystyrene multi-well vessels used to grow, treat, and analyze cells in parallel across multiple conditions in a single vessel. All formats are E-beam sterilized to SAL 10⁻⁶, certified DNase/RNase-free and non-pyrogenic, and individually wrapped in peelable film. Choose TC-treated flat-bottom plates for adherent mammalian cell culture, assays, and transfection; choose non-treated plates for suspension, hematopoietic, and hybridoma cells; choose glass-bottom plates for live-cell confocal and high-resolution fluorescence imaging.
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<h2 class="p1">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="1rzg2e5" data-start="0" data-end="138"><a href="cell-culture-plates/non-treated-cell-culture-plates">Non-Treated Cell Culture Plates:</a> Designed for suspension cultures and non-adherent cell lines where cell attachment is not required.</li>
<li data-section-id="1f2n3q2" data-start="140" data-end="295"><a href="cell-culture-plates/treated-cell-culture-plates">Treated Cell Culture Plates:</a> Tissue-culture-treated surface promotes reliable attachment and growth of adherent cells for routine culture and assays.</li>
<li data-section-id="1dji5w9" data-start="297" data-end="442" data-is-last-node=""><a href="cell-culture-plates/glass-bottom-cell-culture-plates">Glass Bottom Cell Culture Plates:</a> Glass-bottom wells provide superior optical clarity for high-resolution microscopy and live-cell imaging.</li>
</ul>
<h2 class="p2">&nbsp;</h2>
<h2 class="p2">How to Choose Cell Culture Plates</h2>
 Well Count by Application Scale 6-well plates (9.4 cm&sup2;/well) are the standard for transfection, siRNA knockdown, Western blot sample preparation, luciferase reporter assays, and colony counting &mdash; any protocol that requires &ge;5 &times; 10⁶ cells per condition. 12-well plates (3.8 cm&sup2;/well) support medium-scale culture for ELISA lysate preparation, flow cytometry staining, and viral infection studies. 24-well plates (1.9 cm&sup2;/well) are versatile for drug dose-response at medium throughput, primary cell culture, and viral plaque assays. 48-well plates (0.95 cm&sup2;/well) bridge 24-well and 96-well scale for intermediate throughput experiments. 96-well plates (0.32 cm&sup2;/well) are the workhorse for high-throughput cell viability (MTS, MTT, CCK-8), cytotoxicity, ELISA, reporter gene, and compound screening assays. 384-well plates are used for ultra-high-throughput screening (HTS), where reagent cost per well drives miniaturization. TC-Treated vs. Non-Treated vs. Glass Bottom TC-treated (vacuum plasma&ndash;treated, contact angle &lt;10&deg;) flat-bottom plates support adherent mammalian cell monolayer formation &mdash; required for HeLa, HEK293 (adherent), CHO-K1, Vero, A549, MCF-7, and primary cells. Non-treated plates maintain a hydrophobic surface that prevents attachment &mdash; used for suspension cell lines (Jurkat, K562, HL-60), hybridoma culture, and spheroid/organoid formation in U-bottom wells. Glass-bottom plates combine a polystyrene plate body with a borosilicate coverglass floor (0.16&ndash;0.19 mm thickness), matching the No. 1.5 coverglass standard required for confocal, TIRF, and super-resolution microscopy. Flat vs. Round (U) vs. V-Bottom Well Geometry Flat-bottom wells provide a flat imaging surface for phase-contrast and fluorescence microscopy, uniform cell monolayer coverage, and reliable OD measurements in plate readers. Round (U)-bottom wells concentrate cells and particles to the well centre by gravity &mdash; used for rosette assays, hemagglutination inhibition (HAI), and hanging-drop spheroid culture. V-bottom wells have a sharp vertex that pellets cells tightly for wash steps in flow cytometry and immunophenotyping panels. Plate Lid and Evaporation Management NEST Scientific cell culture plates ship with a clear polystyrene lid. For high-throughput assays running 72&ndash;96 hours, edge-well evaporation in 96-well and 384-well formats can introduce artefacts; NEST Low Evaporation EDGE plates have an inter-wall design that reduces edge-well evaporation by maintaining humidity in the peripheral wells during long incubations. 
<h2 class="p2"> Specifications Context</h2>
 NEST Scientific cell culture plates are manufactured from 100% virgin polystyrene in ISO 8 (Class 10,000) cleanrooms. TC treatment uses vacuum plasma technology; the lot-release criterion is contact angle &lt;10&deg;. All plates conform to ANSI/SLAS 1-2004 through 4-2004 footprint and well-position standards (SBS format), ensuring compatibility with automated liquid handling platforms, plate readers, and automated incubators. E-beam sterilization to SAL 10⁻⁶. Plates are individually wrapped in peelable Tyvek-equivalent film for GMP cleanroom entry; lids are attached. Alphanumeric well markings (A&ndash;H rows, 1&ndash;12 columns for 96-well) are standard across all formats. NEST Scientific is ISO 9001:2015 and ISO 13485 certified with FDA registration. As of 2026, MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center. 
Pick your format, culture your cells, and get consistent results every time. <a href="https://mbpinc.net/contact-us">Contact MBP </a>and get a tailored quote today!

<h2 class="p1"> What are Non-Treated Cell Culture Plates?</h2>
 Non-treated cell culture plates are sterile, single-use multi-well polystyrene plates whose well surfaces retain the original hydrophobic, unmodified polystyrene character &mdash; no plasma, corona, or chemical treatment is applied. The hydrophobic surface (contact angle ~75&ndash;90&deg;) prevents cell attachment, making these plates the correct choice for suspension cell lines, hematopoietic cells, hybridoma culture, spheroid and organoid formation, and any experiment where surface adhesion would alter the cell phenotype or experimental readout. Non-treated plates are the correct default for all suspension-growing cell lines and for any 3D culture format &mdash; spheroids, organoids, embryoid bodies &mdash; where surface attachment is undesired.
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<h2 class="p1">What you will find:</h2>
&nbsp;
<ul>
<li>6, 12, 24, 48, and 96 well formats for different workflows</li>
<li>Untreated surfaces with no coating or modification</li>
<li>Clear plates for easy observation and monitoring</li>
<li>Uniform wells for consistent sample handling</li>
<li>Sterile options to maintain clean conditions</li>
<li>Lids designed to reduce contamination and evaporation</li>
</ul>
<h2 class="p1"> How to Choose Non-Treated Cell Culture Plates</h2>
 Suspension Cell Lines Requiring Non-Treated Plates Non-treated flat-bottom plates support maintenance and assays for Jurkat T-cells, K562 erythroleukemia, HL-60 promyelocytes, Raji B-cells, THP-1 monocytes, MOLM-13, and Daudi cells. Primary hematopoietic cells &mdash; PBMCs, CD34⁺ progenitors, NK cells, and bone marrow isolates &mdash; require non-treated surfaces to prevent integrin-triggered differentiation signals from TC-treated polystyrene. For hybridoma culture during HAT selection, non-treated 96-well U-bottom plates are the standard format. U-Bottom vs. Flat-Bottom for Non-Treated Plates Non-treated flat-bottom plates are the standard for suspension cell viability assays, cytotoxicity screens, and ELISA from non-adherent lysates, where wells need a uniform optical path and a flat imaging surface. Non-treated U-bottom 96-well plates concentrate non-adherent cells to the well center by gravity, producing cell pellets that are easier to aspirate during wash steps &mdash; essential for immunophenotyping panels with multiple antibody labeling and wash cycles before flow cytometry. Spheroid and 3D Culture in Non-Treated Plates Non-treated round-bottom (U-bottom) 96-well plates are widely used for hanging-drop spheroid formation and gravity-aggregated tumor spheroid culture. The hydrophobic, non-adhesive well surface combined with U-bottom geometry causes cells to aggregate at the well vertex by gravity, forming compact spheroids over 24&ndash;72 hours without scaffolding. Spheroids formed in non-treated U-bottom 96-well plates are directly measurable in the plate using a plate reader or confocal microscope with an appropriate working distance. Assay Compatibility Non-treated plates are fully compatible with cell viability reagents (CellTiter-Glo, CellTiter-Aqueous MTS, PrestoBlue), cytokine ELISA from cell supernatants, luciferase reporter assays from suspension-cell lysates, and flow cytometry sample preparation. For assays requiring a flat optical surface with minimal well-to-well distance variation, flat-bottom non-treated plates are preferred over U-bottom. Sterility and Purity Grade NEST Scientific non-treated plates are E-beam sterilized to SAL 10⁻⁶, certified DNase/RNase-free, non-pyrogenic, and individually wrapped in peelable sterile film. They are manufactured from 100% virgin polystyrene in ISO 8 cleanrooms under ISO 9001:2015 and ISO 13485 quality management. No additional surface chemistry or coating is applied; the non-treated specification is confirmed by water contact angle measurement per NEST lot release protocol. 
<h2 class="p1"> Specifications Context</h2>
 NEST Scientific non-treated plates are manufactured from 100% virgin polystyrene in ISO 8 cleanrooms; the non-treated well surface has a water contact angle of ~75&ndash;90&deg; (hydrophobic) confirmed per lot release. All plates conform to ANSI/SLAS SBS footprint standards (127.76 &times; 85.48 mm), ensuring compatibility with automated liquid handlers and plate readers. Alphanumeric well coordinates are printed on all plates &ge;12-well format. Package box double functions as a reagent reservoir for multi-channel pipette operations per NEST design. As of 2026, NEST Scientific non-treated plates in all core formats are stocked by MBP. 
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Use non-treated plates when you want full control over how your cells interact with the surface. Keep conditions simple. Keep results predictable. <a href="https://mbpinc.net/contact-us">Contact the MBP team</a> now to get a tailored quote.

Non-Treated Cell Culture Plates

96 Well Cell Culture Plate, Non-Treated, Sterile

701311 by NEST Scientific is a 96-well flat-bottom cell culture plate in white, non-treated USP Class VI polystyrene, E-beam sterile to SAL 10⁻⁶. The hydrophobic non-treated surface suits suspension cell lines, spheroid formation, bacteriological work, and luminescence assays. Features a low-evaporation lid, corner feet, raised rim, and SBS/ANSI footprint. Ships 1/bag, 100/case.

96 Well Cell Culture Plate, White, Flat bottom, Non-Treated, Sterile, 1/pack, 100/cs

96 Well Cell Culture Plate, U-bottom, Non-Treated, sterile 1/pk, 100/cs

96 Well Cell Culture Plate, V-bottom, Non-Treated, sterile 1/pk, 100/cs

BioFactory Accessories

<h2 dir="ltr"> What are Accessories for NEST BioFactory&trade; Systems?</h2>
 NEST BioFactory&trade; accessories are the replacement caps, silicone tubing, vent filters, overcaps, and pre-assembled closed-system cap assemblies used to configure BioFactory units for closed aseptic liquid transfer, gas exchange management, and multi-unit serial connections in cGMP and research-scale bioprocess workflows. The accessory set enables the BioFactory narrow-mouth cap to function as a closed-system liquid transfer port &mdash; media, wash buffer, trypsin, and cell harvest supernatant are transferred in and out through the silicone tubing connection without opening the vessel. Accessories for the BioFactory are required when operating the BioFactory as a closed system; for simple open-pour workflows using wide-mouth caps, standard vent and plug seal caps are the only accessories needed.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="color: #000000;" aria-level="1">
BioFactory media transfer caps
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Sterile tubing and connector sets
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Fluid transfer adapters for cell culture vessels
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
BioFactory bottle caps and closures
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Sterile quick-connect systems
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Liquid handling accessories
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Vessel handling and support tools
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Closed-system connectors for aseptic processing
</li>
</ul>
<h2 dir="ltr"> Key BioFactory Accessory Categories</h2>
 BioFactory Cap with Silicone Tube (CPC or Luer Connector) The BioFactory cap with silicone tube is the primary closed-transfer accessory: an adaptor cap that fits the narrow-mouth port of the BioFactory unit, pre-assembled with 80 cm of silicone tubing and a terminal connector &mdash; either a male CPC (clamped pressure connector / quick-connect) or a standard Luer lock &mdash; for coupling to peristaltic pumps, other BioFactory units, centrifuge tubes, or PETG media bottle assemblies. Two tubing I.D. &times; O.D. configurations are available: 1/4" I.D. &times; 3/8" O.D. and 3/8" I.D. &times; 5/8" O.D., covering standard peristaltic pump head bore sizes. The female sealing cap included with each assembly caps the connector between uses, maintaining sterility. Overcaps: Vented and Solid Overcaps are secondary dust and contamination barriers placed directly on top of the BioFactory adaptor caps during culture incubation. The vented overcap contains a 0.22 &micro;m hydrophobic breathable membrane, allowing gas exchange through the cap stack while blocking particles and microbial contamination &mdash; used during active shaker or orbital incubation when the unit's primary cap is an adaptor cap rather than a vented cap. The solid overcap provides an additional dust and contamination seal with no gas exchange &mdash; used for short-term storage or transport outside the incubator. SPT-50 Silicone Hose SPT-50 silicone hose (3/8" I.D. &times; 5/8" O.D., 50 inches / 15 meters) is the bulk tubing used to connect multiple BioFactory units in series, connect BioFactory ports to peristaltic pump heads, or extend the reach of the transfer cap assembly. SPT-50 tubing is manufactured from silicone, is individually wrapped and sterile, and passes dissolution testing per BPOG (BioPhorum Operations Group) criteria per NEST product documentation, supporting its use in biopharmaceutical production environments. Premium Accessory Package NEST offers a BioFactory Accessory Premium Package that includes 2 different adaptor caps, 1 hose clamp, 1 adaptor connector, 1 vent filter (50 mm), 1 additional hose clamp, and 15 m of SPT-50 silicone hose &mdash; all individually wrapped and sterile in a single kit. This package provides all components needed to set up a closed-system BioFactory media management workflow from a single kit, reducing the number of individual part orders required. Vent Filters for Gas Exchange Management For BioFactory units using adaptor caps rather than standard vented caps, 50 mm inline vent filters (0.22 &micro;m hydrophobic PTFE membrane) are installed on the air inlet/outlet tubing line to maintain sterile gas exchange during media addition, harvest, and incubation. The vent filter is rated for high flow rates without creating excessive back-pressure that would resist liquid movement through the system. Compatibility: Narrow-Mouth BioFactory Units Only All BioFactory accessory caps and tubing assemblies are designed exclusively for the narrow-mouth BioFactory configuration. Wide-mouth BioFactory units use standard wide-mouth vent and plug seal caps only; they are not compatible with accessory cap assemblies. Confirm that your BioFactory units have narrow-mouth ports before ordering the accessory cap set. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST BioFactory&trade; accessory silicone tubing and cap assemblies are manufactured from USP Class VI silicone (TPE/silicone) and polycarbonate or PP cap bodies, individually wrapped and sterile. All components have passed dissolution testing per BPOG dissolution test criteria conducted by a certified third-party laboratory per NEST Scientific documentation. The standard Luer head connections conform to ISO 594 / GB/T 1962 for reliable compatibility with standard laboratory fittings. Vent filters use a 0.22 &micro;m hydrophobic PTFE membrane; overcaps use a 0.22 &micro;m hydrophobic membrane. NEST BioFactory accessories are carried by MBP alongside BioFactory units. 
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Need the right BioFactory setup? <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact MBP today</a> for technical assistance.

Biofactory System Accessories

Product Literature

NEST BioFactory Adaptor Caps Specifications

Adaptor Caps for BioFactories, Individually Wrapped, Sterile

NEST Scientific 740201 sterile adaptor caps for BioFactory multi-layer cell culture vessels, individually wrapped. Enables closed-system tubing and hose connections to NEST BioFactory ports for media exchange and harvest in large-scale adherent cell culture. Multiple cap types and materials available.

Adaptor Cap Wide Mouth to Narrow Mouth for BioFactories, Individually Wrapped, Sterile, 1/pk, 10/cs

Adaptor Cap Wide Mouth to 3/8" ID (9.5 mm) Hose for BioFactories, PP, Individually Wrapped, Sterile, 1/pk, 10/cs

Adaptor Cap Wide Mouth to 1/4" (5.8 mm) ID Hose for BioFactories, PP, Individually Wrapped, Sterile, 1/pk, 10/cs

Adaptor Cap Wide Mouth to 3/8" ID (9.5 mm) Hose for BioFactories, HDPE, Individually Wrapped, Sterile, 1/pk, 10/cs

Adaptor Connector to 3/8 Inches(9.5 mm) Hose for BioFactories, Individually Wrapped, Sterile, 1/pk, 10/cs

NEST Scientific 741001 is a sterile individually wrapped adaptor connector for BioFactory multi-layer cell culture vessels, sized for 3/8-inch (9.5 mm) inner-diameter hose lines. Supplies the hose-to-BioFactory port connection in closed-system media transfer and harvest assemblies. 1/pk, 10/cs.

Vacuum Filtration

<h2 dir="ltr">What are Vacuum Bottle-Top Filter Systems?</h2>
 Vacuum bottle-top filter systems are single-use, sterile filtration assemblies consisting of a graduated polystyrene funnel with an integrated membrane filter bonded to a polystyrene receiver bottle, connected by a hose-port adaptor to a house vacuum line or benchtop vacuum pump. The applied negative pressure (&minus;50 to &minus;100 kPa) draws liquid through the membrane into the receiver bottle, which is sealed after filtration for immediate use or cold-room storage.&nbsp;
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<h2 dir="ltr">What you will find:</h2>
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50mL Vacuum Filtration Units for small-scale sterile sampling
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150mL Vacuum Filtration Systems for rapid reagent preparation
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250mL Filtration Units for routine buffer sterilization
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500mL Vacuum Filtration Cups for mid-scale media processing
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1000mL Large Volume Filtration for industrial-scale batching
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Sterile Bottle-Top Filters for secure aseptic transfer
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<h2 dir="ltr"> How to Choose Vacuum Filtration Systems</h2>
 PES vs. PVDF Membrane for Vacuum Filtration PES membranes provide low protein binding (~1&ndash;2 &micro;g/cm&sup2;), high flow rates, and broad aqueous-solution compatibility &mdash; the standard for routine DMEM, RPMI, HBSS, PBS, and supplement-containing media sterilization. PVDF membranes have slightly lower protein binding than PES and greater chemical resistance to mild organic solvents, mild acids, and alkaline solutions. Use PVDF when filtering protein-rich solutions (FBS, antibody preparations, conditioned medium with high protein content) or when mild organic solvents are present in the filter solution. Volume Selection 250 mL systems (50 mm diameter membrane, 50 mL funnel) suit small media aliquots, supplement solutions, and transfection reagent preparations. 500 mL systems (90 mm diameter membrane, 150 mL funnel) are the routine lab workhorse for standard 500 mL media bottle preparation and FBS filtration. 1,000 mL systems (90 mm diameter membrane, 350 mL funnel) handle full-liter media preparations and large-volume buffer sterilization. All NEST systems include a pre-attached, graduated polystyrene receiver bottle in the corresponding volume. Pore Size: 0.10 &micro;m vs. 0.22 &micro;m vs. 0.45 &micro;m 0.22 &micro;m is the sterilizing grade per USP &lt;1229.2&gt; and ICH Q9: retains all bacteria and fungi; used for routine cell culture media, PBS, and buffer preparation. 0.45 &micro;m is the clarification grade: used as a pre-filtration step before 0.22 &micro;m to remove large particulates from turbid or high-biomass samples (FBS, conditioned media, cell culture supernatants, crude lysates), extending 0.22 &micro;m filter throughput by 3&ndash;5&times; on difficult samples. 0.10 &micro;m is the mycoplasma-removal grade: required when preparing media for Mycoplasma-sensitive primary cell culture or when processing serum or supplementary reagents that may harbor Mycoplasma species. Note that 0.10 &micro;m filtration is substantially slower than 0.22 &micro;m due to the tighter membrane pore structure. Practical Tips for Maximum Throughput Pre-wet the membrane by briefly passing 5&ndash;10 mL of sterile water through before the sample &mdash; this wets the hydrophilic PES or PVDF membrane and reduces initial resistance. For FBS and other viscous solutions, pre-filter through 0.45 &micro;m before the 0.22 &micro;m step. Maintain a vacuum at &minus;50 to &minus;80 kPa; excessive vacuum (&gt; &minus;100 kPa) can shear fragile membrane pores and increase non-specific protein adsorption. Do not autoclave the assembled bottle-top filter &mdash; PES and PS components are not rated for autoclave sterilization. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific vacuum bottle-top filters are manufactured from polystyrene funnel and receiver bottle components with PES or PVDF membranes. PES membranes have documented low protein binding (~1&ndash;2 &micro;g/cm&sup2;) per NEST scientific USA FAQ. Membrane diameter: 250 mL volume &rarr; 50 mm; 500 mL and 1,000 mL &rarr; 90 mm. The hose connector design accommodates various standard vacuum hose diameters. NEST PES 0.22 &micro;m vacuum filters are described as comparable in performance to leading brands, including Corning Stericup, Thermo Fisher Scientific, and Sartorius, per NEST Scientific USA's product page. All NEST vacuum filters are sterile and non-pyrogenic per the lot certificate. NEST Scientific vacuum bottle-top filters are stocked by MBP in all core SKUs. 
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Because your research should move faster than a slow-dripping filter. <a href="https://mbpinc.net/contact-us" title="Contact the Molecular Biology Products Inc. team" style="color: #236fa1;">Contact the Molecular Biology Products Inc. team</a> for a quote before your deadlines catch up with you.

Adaptor for Vaccum Filtration System, 45mm to 38mm, 1/pk, 10/cs

334591 by NEST Scientific is a polypropylene adaptor that converts a 45 mm vacuum filtration port to a 38 mm connection, compatible with NEST disposable vacuum filtration systems in 250 mL, 500 mL, and 1000 mL formats. Supplied 1/pk, 10/cs for lab and bioprocessing filtration setups.

Bi-directional Transfer Cap with Silicon Tube

C30821-EAC050B by NEST Scientific — bi-directional transfer cap for 125 mL Erlenmeyer flasks with 50 cm silicone tube (1/8&quot; ID &times; 1/4&quot; OD), male CPC connector with female sealing cap, and 0.22 &mu;m (&Phi;42mm) vent filter. E-beam sterile (SAL 10-6), cap only, 1/pk, 4/cs.

125 mL Erlenmeyer Flask Bi-directional Transfer Cap with Silicon Tube (50cm 1/8" ID 1/4" OD), Male CPC Connector with Female Sealing Cap, Vent Filter (0.22 μm Φ42mm), without Flask, Sterile, 1/pk, 4/cs

125 mL Erlenmeyer Flask Bi-directional Transfer Cap with Silicon Tube (50cm 1/8" ID 1/4" OD), Male Luer Lock Connector with Luer Cap, Vent Filter (0.22 μm Φ42mm), without Flask, Sterile, 1/pk, 4/cs

250mL Erlenmeyer Flask Bi-directional Transfer Cap with Silicon Tube (50cm 1/8" ID 1/4" OD), Male CPC Connector with Female Sealing Cap, Vent Filter (0.22 μm Φ42mm), without Flask, Sterile, 1/pk, 4/cs

250 mL Erlenmeyer Flask Bi-directional Transfer Cap with Silicon Tube (50cm 1/8" ID 1/4" OD), Male Luer Lock Connector with Luer Cap, Vent Filter (0.22 μm Φ42mm), without Flask, Sterile, 1/pk, 4/cs

500 mL Erlenmeyer Flask Bi-directional Transfer Cap with Silicon Tube (50cm 1/8" ID 1/4" OD), Male CPC Connector with Female Sealing Cap, Vent Filter (0.22 μm Φ42mm), without Flask, Sterile, 1/pk, 4/cs

500 mL Erlenmeyer Flask Bi-directional Transfer Cap with Silicon Tube (50cm 1/8" ID 1/4" OD), Male Luer Lock Connector with Luer Cap, Vent Filter (0.22 μm Φ42mm), without Flask, Sterile, 1/pk, 4/cs

1000 mL Erlenmeyer Flask Bi-directional Transfer Cap with Silicon Tube (50cm 1/8" ID 1/4" OD), Male CPC Connector with Female Sealing Cap with, Vent Filter (0.22 μm Φ42mm), without Flask, Sterile, 1/pk, 4/cs

1000 mL Erlenmeyer Flask Bi-directional Transfer Cap with Silicon Tube (50cm 1/8" ID 1/4" OD), Male Luer Lock Connector with Luer CapVent Filter (0.22 μm Φ42mm), without Flask, Sterile, 1/pk, 4/cs

BioFactory Accessory Packages Components

BioFactory Accessory Package

ZME000B by NEST Scientific — BioFactory Accessory Package. Sterile kit includes: hose clamp, 0.22 &mu;m vent filter (62mm), silicone tube (80cm, 1/4&quot; ID &times; 3/8&quot; OD), male CPC connector with sealing cap, and 2 silicone rings. 1/pk, 4/cs. BioFactory vessel sold separately.

BioFactory Accessory Lite Package, Hose Clamp*1+50 mm Vent Filter*1+15 cm SPT-50 Hose*1+Silicone Ring*2, Sterile, 1/pk, 2/cs

BioFactory Accessory Basic Package,Hose Clamp*1+50 mm Vent Filter+15 cm SPT-50 Hose*1+Adaptor Connector*1+Silicone Ring*2, Sterile, 1/pk, 2/cs (Old No.: 745021)

BioFactory Accessory Premiμm Package, 2 different Adaptor Caps*1+Hose Clamp*1+Adaptor Connector*1 +50 mm Vent Filter*1+Hose Clamp*1+15 m SPT-50 Hose*1, Individually Wrapped, Sterile, 1/pk, 1/cs

745001 by NEST Scientific is a sterile BioFactory Accessory Complete Package containing 2 adaptor caps, 2 hose clamps, 1 adaptor connector, 1 × 50 mm vent filter, and 15 m SPT-50 hose — individually wrapped, ISO 8 clean room manufactured, USP Class VI materials, ISO 9001/13485 certified. Provides a complete closed-system fluid-transfer setup for NEST BioFactory cell culture vessels. Available through MBP, a registered vendor to Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, Eurofins, and many other institutes across the USA.

BioFactory Adaptor Caps and Overcaps Specifications

BioFactory Adaptor Caps and Overcaps

740213 by NEST Scientific is a sterile BioFactory adaptor cap assembly combining the 740201 wide-mouth-to-narrow-mouth HDPE adaptor cap with a vented overcap fitted with a 0.22 μm hydrophobic breathable membrane. It enables aseptic ventilation during liquid transfer on NEST BioFactory 10-chamber and 40-chamber cell culture systems. Individually wrapped, ISO 9001 and ISO 13485 certified. Pack size: 1/pk, 10/cs.

Adaptor Cap(740201) Pre Installed with a Vented Overcap for BioFactories, Individually Wrapped, Sterile, 1/pk, 10/cs

Solid Overcap for BioFactories, Adaptable for 740201, Individually Wrapped, Sterile, 1/pk, 20/cs

Vented Overcap for BioFactories, Adaptable for 740201, Individually Wrapped, Sterile, 1/pk, 20/cs

United States

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