Azura Reagents

SYBR/Dye-Based

<h2 dir="ltr">What is a SYBR Green Dye-Based Master Mix?</h2>
 A SYBR Green dye-based master mix is a 2x, ready-to-use formulation designed for dye-based, quantitative amplification of DNA targets, including cDNA and genomic DNA, by real-time PCR. SYBR Green dye binds to double-stranded DNA formed during amplification, and the resulting fluorescence increases proportionally to the quantity of double-stranded product, providing a real-time readout of amplification progress. Choose a SYBR Green dye-based master mix for cost-effective, flexible quantification across any primer pair and target combination, especially when running many different assays without the overhead of probe design.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="ic0lb3" data-start="180" data-end="319">SYBR Green dye-based qPCR master mixes for real-time quantification of DNA and cDNA targets across a wide range of research applications.</li>
<li data-section-id="1yz7ajv" data-start="320" data-end="449">Fast qPCR formulations with HiROX and LoROX options to support compatibility with different real-time PCR instrument platforms.</li>
<li data-section-id="1fbhq1p" data-start="450" data-end="586">Blue tracking dye master mixes, including AzuraView&trade; GreenFast and Accuris qMAX Green formats, for improved reaction setup visibility.</li>
<li data-section-id="1787obc" data-start="587" data-end="726">One-step SYBR Green RT-qPCR mixes that combine reverse transcription and real-time PCR in a single tube for RNA quantification workflows.</li>
<li data-section-id="1g0jipr" data-start="727" data-end="852">Ready-to-use 2x master mixes formulated for reproducible qPCR setup while reducing pipetting steps and workflow complexity.</li>
</ul>
<h2 dir="ltr"> How to Choose a SYBR Green Dye-Based Master Mix</h2>
 Dynamic Range and Sensitivity Look for master mixes offering a wide linear dynamic range -- some formulations provide precise measurement across approximately six logs of dynamic range, enabling accurate detection across both low- and high-concentration samples in the same assay design. Primer Melting Temperature Flexibility SYBR Green master mixes are commonly formulated to provide flexibility for primers with melting temperatures (Tm) in the range of approximately 55-65 &deg;C, reducing the need for extensive primer-specific optimisation when bringing new assays online. Bench-Top Stability Dual hot-start mechanisms in some SYBR Green master mixes provide extended bench-top stability -- up to 72 hours at room temperature in the dark in some formulations -- with single melt curves confirmed at both initial and post-incubation time points, supporting automated and batched workflows. Carryover Contamination Prevention Formulations that include uracil-DNA glycosylase (UDG) and dUTP help ensure leftover DNA residue from previous reactions is not re-amplified in subsequent experiments, a useful feature for high-throughput repeat assays. Instrument and Workflow Compatibility Broad instrument compatibility - including standard and fast cycling modes -- and compatibility with a variety of reverse transcriptase enzymes (for RT-qPCR workflows using a separately generated cDNA) widen the range of platforms a single master mix can support. 
<h2 dir="ltr"> Specifications Context</h2>
 SYBR Green dye is a fluorescent double-stranded DNA-binding dye that tracks amplification progress in real-time PCR: as the reaction proceeds, the dye binds newly synthesised dsDNA at each extension step, and the resulting fluorescence increase is proportional to product quantity. Because SYBR Green is not PCR-inhibitory and works with any primer-template combination, it provides accurate, reproducible results with high sensitivity across a broad dynamic range and gives consistent results across different real-time PCR platforms - one reason dye-based chemistry remains cost-effective compared to probe-based systems, which require a different labelled probe for each target sequence. SYBR Green master mixes are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light; lot-specific CoAs are available on request. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get high-yield SYBR/Dye-Based real-time PCR reagents for your lab.

AzuraQuant Green Fast qPCR Mix HiRox

AzuraQuant Green Fast qPCR Mix

MSDS

AzuraQuant™ Green Fast qPCR Mix HiRox

AzuraQuant™ Green Fast qPCR Mix HiRox is available in the following variants: AZ-2001, AZ-2005, AZ-2020, AZ-2050.AzuraQuant™ Green Fast qPCR Mix HiRox (AZ-2001) by Azura Genomics is a 2× hot-start SYBR-channel qPCR master mix with a high-ROX passive reference dye, optimised for Applied Biosystems and QuantStudio platforms. Compatible with standard and fast cycling protocols. Available in 100, 500, 2,000, and 5,000 reaction sizes from MBP Inc.

Manufacturer

AzuraQuant Green Fast qPCR Mix HiRox, 100 reactions

AzuraQuant Green Fast qPCR Mix HiRox 500 Reactions

AzuraQuant Green Fast qPCR Mix HiRox 2,000 reactions

AzuraQuant Green Fast qPCR Mix HiRox 5,000 reactions

User Manual

AzuraQuant Green Fast qPCR Mix - Product Sheet

qPCR Reagent Compatibility Tool

AzuraQuant™ Green Fast qPCR Mix LoRox

The AzuraQuant™ Green Fast qPCR Mix LoRox by Azura Genomics is AzuraQuant™ Green Fast qPCR Mix LoRox, 2× master mix with Azura HS Taq, SYBR Green channel, low ROX. 20 µL standard reactions, fast-cycling protocol (95°C 2 min / 5 sec denaturation / 20–30 sec anneal-extension). Stored at −20°C, 18-month shelf life. Available in 100 reactions (AZ-2101), 500 reactions (AZ-2105), 2,000 reactions (AZ-2120), and 5,000 reactions (AZ-2150).

AzuraQuant Green Fast qPCR Mix LoRox 100 reactions

AzuraQuant Green Fast qPCR Mix LoRox 500 Reactions

AzuraQuant Green Fast qPCR Mix LoRox 2,000 reactions

AzuraQuant Green Fast qPCR Mix LoRox 5000 reactions

cDNA Synthesis Kits

<h2 dir="ltr"> What is a cDNA Synthesis Kit?</h2>
 A cDNA synthesis kit provides the complete set of reagents - reverse transcriptase enzyme, reaction buffer, dNTPs, RNase inhibitor, and primers - needed to perform first-strand reverse transcription, converting an RNA sample into a cDNA pool. Unlike one-step RT-PCR kits, which combine RT and PCR in a single reaction with gene-specific primers, a cDNA synthesis kit performs reverse transcription as a standalone step, producing a cDNA template that can be used across multiple subsequent PCR or qPCR reactions targeting different genes. Choose a cDNA synthesis kit when one RNA sample needs to be analysed for multiple gene targets, or when downstream qPCR requires a separately-generated, quality-checked cDNA template. 
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="1o7fvd2" data-start="74" data-end="228">First-strand cDNA synthesis kits for RNA-to-cDNA conversion in gene expression workflows, including AzuraQuant&trade; and AzuraFlex&trade; cDNA synthesis kits</li>
<li data-section-id="z40sk0" data-start="229" data-end="395">Standard cDNA synthesis kits for routine transcriptional analysis and downstream PCR applications, including Accuris qMAX and 1st cDNA Synthesis Kit (FS01-00)</li>
<li data-section-id="1yvybqf" data-start="396" data-end="521">Flexible and high-efficiency cDNA synthesis systems designed to support a range of RNA inputs and experimental conditions</li>
<li data-section-id="sl2teb" data-start="522" data-end="599">miRNA-specific cDNA synthesis solutions for small RNA expression analysis</li>
<li data-section-id="17gz9kt" data-start="600" data-end="770" data-is-last-node="">One-step and integrated RT-based systems and master mixes supporting streamlined reverse transcription workflows, including OneScript Plus and OneScript Hot RT kits</li>
</ul>
<h2 dir="ltr"> How to Choose a cDNA Synthesis Kit</h2>
 Priming Strategy Oligo(dT) primers target the poly-A tail of mRNA, enriching specifically for mRNA-derived cDNA and reducing genomic DNA or rRNA background. Random primers (commonly hexamers) prime throughout the RNA template regardless of sequence, useful for templates lacking a poly-A tail (such as bacterial RNA) or when full-length cDNA coverage, including 5' regions, is needed. Some kits offer a blend of both primer types for balanced coverage. Reverse Transcriptase Included Kits built around M-MLV RNase H-minus reverse transcriptase favour longer, more complete cDNA products, while kits using enhanced-thermostability or inhibitor-resistant reverse transcriptases may better handle GC-rich templates or minimally purified RNA samples. Genomic DNA Elimination Step Some cDNA synthesis kits include a separate genomic DNA elimination (gDNA wipeout) step performed before reverse transcription -- important for accurate gene expression quantification, since residual genomic DNA can contribute false signal in downstream qPCR if primers span regions present in both genomic DNA and the cDNA target. Reaction Volume and Throughput cDNA synthesis kits are available in formats scaled for individual reactions through to high-throughput 96-well formats, allowing labs to match kit format to typical sample batch sizes without excessive reagent waste. Downstream Application Compatibility The resulting cDNA pool should be compatible with the intended downstream application -- standard PCR, qPCR with SYBR Green or probe-based detection, or digital PCR -- confirm that the kit's buffer system does not interfere with the chosen downstream detection chemistry. 
<h2 dir="ltr"> Specifications Context</h2>
 In a two-step workflow, the first step synthesises first-strand cDNA using a reverse transcriptase such as M-MLV-RT with the chosen priming strategy, and the resulting cDNA pool can then be used in multiple separate PCR or qPCR reactions targeting different genes - a key advantage when a single RNA sample needs to be analysed for several targets, since the cDNA synthesis step is performed only once. Genomic DNA elimination steps, where included, are typically performed as a brief pre-treatment with a DNase before the reverse transcription reaction itself begins. cDNA synthesis kit components - enzyme, buffer, dNTPs, and RNase inhibitor - are typically stored at &minus;20 &deg;C; RNase-free water and RNase inhibitor handling throughout the workflow are recommended to prevent RNA template degradation before synthesis.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get high-precision cDNA synthesis kits for your lab.

Product Literature

AzuraQuant™ II cDNA Synthesis Kit

Azura Genomics AzuraQuant™ II cDNA Synthesis Kit: HighTherm™ II RT, 4 pg–2 µg total RNA, 25 min, combined random hexamer + oligo(dT) primers. AZ-2501 (25), AZ-2504 (100), AZ-2520 (500 rxns).

AzuraQuant II cDNA Synthesis Kit 25 Reactions

AzuraQuant II cDNA Synthesis Kit 100 Reactions

AzuraQuant II cDNA Synthesis Kit 500 Reactions

Direct PCR Master Mixes

<h2>What Are Direct PCR Master Mixes?</h2>
&nbsp;
Direct PCR master mixes are specially formulated 2x PCR reagents designed to amplify nucleic acid targets directly from crude biological samples such as blood, buccal swabs, tissue punches, cultured cells, or other minimally processed material without a separate DNA or RNA extraction step. This is achieved through inhibitor-tolerant polymerase formulations, often combined with proprietary buffer additives that counteract the effects of hemoglobin, melanin, polysaccharides, and other common PCR inhibitors present in crude lysates.&nbsp;Choose a direct PCR master mix for high-throughput genotyping or rapid screening applications where eliminating the extraction step provides meaningful time and cost savings, provided the application tolerates the typically modest reduction in amplification efficiency compared to extracted-template PCR.
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<h2>What You Will Find</h2>
&nbsp;
<ul>
<li>Direct PCR &amp; Fast Extraction Kits: Streamlined solutions such as the Flash-Extract&trade; PCR Kit and Accuris Fast Extraction PCR Kit that enable rapid PCR workflows from minimally processed samples, reducing preparation time and simplifying genotyping applications.</li>
<li>Genotyping Kits: Specialized products including the Azura&trade; Mouse Genotyping Kit and Accuris 1 Hour Mammalian Genotyping Kit, designed for reliable genotyping workflows using tissue, tail, ear, or other biological samples.</li>
</ul>
&nbsp;
<h2>How to Choose a Direct PCR Master Mix</h2>
&nbsp;
Sample Type Compatibility
Direct PCR formulations vary in the crude sample types they are validated for. Blood, tissue, cells, and swabs each present different inhibitor profiles, so confirm the master mix is validated for your specific sample type before adopting it for a new workflow.
Inhibitor Tolerance Mechanism
Inhibitor tolerance is typically achieved through a combination of enzyme engineering and buffer additives that bind or neutralize inhibitory compounds. The specific combination affects which inhibitor types, such as heme from blood or melanin from pigmented tissue, the mix is best suited to handle.
Lysis Step Requirements
Some direct PCR master mixes require a brief lysis step, such as a short heat or detergent treatment, before amplification, while others are formulated for direct addition of the crude sample to the PCR reaction. Confirm the workflow complexity matches your throughput needs.
Amplicon Size and Application
Direct PCR is well established for short-to-moderate amplicon genotyping and screening applications. Very long amplicons or applications requiring maximum yield from limited template may still benefit from a brief extraction step even when a direct-compatible mix is available.
Comparison to Extraction-Based PCR
Direct PCR master mixes generally show good performance for routine genotyping but may exhibit somewhat reduced sensitivity or yield compared to PCR from purified template. For applications where extraction-free convenience outweighs a modest performance trade-off, direct PCR offers substantial time savings, particularly at high sample throughput.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Direct PCR master mixes enable PCR directly from crude samples through heat-stable polymerases tolerant of inhibitor compounds and lysis buffer components, combined with optimized buffer systems designed to neutralize hemoglobin, melanin, polysaccharides, and other inhibitors commonly found in blood, tissue, and plant material. The time savings from eliminating extraction are most significant in high-throughput genotyping pipelines, where extraction across hundreds of samples can represent a substantial fraction of total workflow time. As with any inhibitor-tolerant formulation, performance can vary by specific sample matrix. Validating a direct PCR master mix against a small panel of representative samples before deploying it across a full study is considered good practice. Direct PCR master mixes are typically stored at -20&deg;C. Lot-specific Certificates of Analysis (CoAs) are available on request.&nbsp;
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Streamline your amplification workflow&mdash;<a href="https://mbpinc.net/contact-us">contact the MBP team</a> today for a quote on our professional Direct PCR solutions.

Mouse Genotyping Kit - Product Sheet

Azura™ Mouse Genotyping Kit

Azura Genomics Mouse Genotyping Kit: rapid lysis buffer (15 min, no columns) + Azura 2× HS Red Mix with direct-gel-loading red dye. ~1 hr total workflow. AZ-1851 (80 rxns), AZ-1855 (400 rxns), AZ-1856 (2,000 rxns).

Azura Mouse Genotyping Kit 80 Reactions

Azura Mouse Genotyping Kit 400 Reactions

Azura Mouse Genotyping Kit 2000 Reactions

AzuraView™ GreenFast qPCR Blue Mix HR

AZ-2401 by Azura Genomics — AzuraView GreenFast qPCR Blue Mix HR; 2X SYBR Green master mix; Azura HS Taq; inert blue tracer dye; increased resistance to freeze-thaw; compatible with all standard qPCR instruments; 100 reactions (1×1 mL).

AzuraView GreenFast qPCR Blue Mix HR 100 Reactions

AzuraView GreenFast qPCR Blue Mix HR 500 Reactions

AzuraView GreenFast qPCR Blue Mix HR 2,000 Reactions

AzuraView GreenFast qPCR Blue Mix HR 5,000 Reactions

AzuraView GreenFast qPCR Blue Mix - Product Sheet

AzuraView™ GreenFast qPCR Blue Mix LR

Azura Genomics AzuraView™ GreenFast qPCR Blue Mix LR: 2× intercalating dye mix with Azura HS Taq, blue tracer dye, low ROX. Standard and fast cycling. AZ-2301 (100), AZ-2305 (500), AZ-2320 (2,000), AZ-2350 (5,000 rxns).

AzuraView GreenFast qPCR Blue Mix LR 100 Reactions

AzuraView GreenFast qPCR Blue Mix LR 500 Reactions

AzuraView GreenFast qPCR Blue Mix LR 2000 Reactions

AzuraView GreenFast qPCR Blue Mix LR 5,000 Reactions

Primer-Probe

<h2 dir="ltr"> What Is a Primer-Probe Master Mix?</h2>
 A primer-probe master mix is a 2x concentrated formulation built around a hot-start Taq DNA polymerase, dNTPs, MgCl2, and optimised buffer, designed for use with hydrolysis-probe detection chemistries such as TaqMan. Unlike SYBR Green dye-based mixes, primer-probe formulations rely on a separately added, fluorescently labelled, sequence-specific probe combined with target-specific primers to generate the detection signal, with specificity coming from both primer and probe sequence matching. Choose a primer-probe master mix when assay specificity is critical, when multiplexing several targets in one well is needed, or when working with an established probe-based assay design.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="qhwnhl" data-start="279" data-end="413">Probe-based qPCR master mixes with HiROX, LoROX, and ROX-free options for compatibility across a range of real-time PCR instruments.</li>
<li data-section-id="1cnihmh" data-start="414" data-end="543">Hot-start polymerase formulations designed to support specific target detection in hydrolysis probe (TaqMan-style) qPCR assays.</li>
<li data-section-id="51tf3n" data-start="544" data-end="643">Blue tracking dye probe master mixes for easier reaction setup and visual pipetting confirmation.</li>
<li data-section-id="cjeacg" data-start="644" data-end="749">2X ready-to-use probe qPCR master mixes containing optimized buffers, dNTPs, and polymerase components.</li>
<li data-section-id="xfwfro" data-start="750" data-end="884">One-step probe RT-qPCR formats that combine reverse transcription and real-time PCR amplification in a single tube for RNA analysis.</li>
</ul>
<h2 dir="ltr"> How to Choose a Primer-Probe Master Mix</h2>
 Reference Dye Inclusion Primer-probe master mixes commonly include ROX as a passive reference dye for signal normalisation across wells on instruments that require it -- confirm whether your real-time PCR instrument needs ROX and whether the chosen master mix includes it or requires separate addition. Multiplexing Capability A core advantage of probe-based chemistry is multiplexing -- detecting multiple targets in a single well using probes labelled with spectrally distinct fluorophores. Confirm the master mix is validated for the number of multiplexed targets your assay requires. Probe Chemistry Compatibility While TaqMan-style hydrolysis probes are the most common, other probe chemistries such as Scorpion primers, exist; confirm the master mix is formulated for compatibility with your specific probe chemistry. Primer and Probe Concentration Guidelines Standard setups commonly use primer pairs at a final concentration around 0.5 &micro;M and probes at 0.25-0.5 &micro;M -- confirm the master mix manufacturer's recommended ranges, since probe concentration affects both signal intensity and background. One-Step vs. Two-Step Application Primer-probe master mixes are used in both two-step workflows (with a separately generated cDNA template) and as the chemistry component within one-step RT-qPCR kits -- confirm which configuration matches your workflow when selecting a product. 
<h2 dir="ltr"> Specifications Context</h2>
 In probe-based qPCR, the 5'-3' exonuclease activity of the Taq DNA polymerase cleaves the dual-labelled probe annealed to the target sequence during amplification, separating a fluorescent reporter from its quencher and generating signal proportional to target abundance -- the mechanism underlying the widely used TaqMan probe system. Because both primer and probe sequence must match the target for signal generation, probe-based assays generally offer higher specificity than dye-based detection, and because probes can be labelled with spectrally distinct fluorophores, multiple targets can be detected in a single well. Standard reaction setup adds primer pairs at approximately 0.5 &micro;M and probes at 0.25-0.5 &micro;M to the 2x master mix. Primer-probe master mixes are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light; lot-specific CoAs are available on request. 
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get optimized Primer-Probe real-time master mixes for your lab.

AzuraView Probe Fast qPCR Mix Blue - Product Sheet

AzuraView™ Probe Fast qPCR Mix Blue

The AzuraView™ Probe Fast qPCR Mix Blue by Azura Genomics is an inhibitor-tolerant 2× master mix formulated for probe-based real-time qPCR, including TaqMan® and molecular beacon assays, with a blue inert tracer dye for visual confirmation of pipetting and compatibility with standard and fast-cycling thermal cyclers. Available in Blue and Colorless formulations in 100-reaction (AZ-2601B/AZ-2601C), 500-reaction (AZ-2605B/AZ-2605C), 2000-reaction (AZ-2620B/AZ-2620C), and 5000-reaction (AZ-2650B/AZ-2650C) pack sizes.

AzuraView Probe Fast qPCR Mix Blue 100 reactions

AzuraView Probe Fast qPCR Mix Colorless 100 reactions

AzuraView Probe Fast qPCR Mix Blue 500 reactions

AzuraView Probe Fast qPCR Mix Colorless 500 reactions

AzuraView Probe Fast qPCR Mix Blue 2000 reactions

AzuraView Probe Fast qPCR Mix Colorless 2000 reactions

AzuraView Probe Fast qPCR Mix Blue 5000 reactions

AzuraView Probe Fast qPCR Mix Colorless 5000 reactions

High-Fidelity DNA Polymerase

<h2>What Are High-Fidelity DNA Polymerases?</h2>
&nbsp;
The development of high-fidelity thermostable DNA polymerases transformed PCR from a tool for detection and amplification into a precise DNA engineering platform. By combining thermostability with 3' to 5' proofreading exonuclease activity, modern high-fidelity enzymes detect and remove misincorporated bases immediately after insertion during DNA synthesis. Polymerase fidelity can significantly affect downstream results, so choosing an enzyme that meets your accuracy and performance requirements is essential.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>High-fidelity DNA amplification with proofreading activity using Azura TruFi&trade; II DNA Polymerase.</li>
<li>Proofreading polymerase formulations with matched reaction buffers, including ExtremeTaq&trade; DNA Polymerase with 5x Reaction Buffer.</li>
<li>Accurate PCR amplification with Accuris High Fidelity DNA Polymerase for cloning, sequencing, and mutagenesis workflows.</li>
<li>High-fidelity enzyme systems featuring proofreading activity, including MegaFi Pro Fidelity DNA Polymerase.</li>
</ul>
&nbsp;
<h2>How to Choose a High-Fidelity DNA Polymerase</h2>
&nbsp;
Enzyme Family Selection
High-fidelity polymerases differ in processivity, extension speed, fidelity, and template compatibility. Selection should consider amplicon length, GC content, template complexity, and downstream application requirements.
Format: Enzyme vs. Master Mix
Individual high-fidelity enzymes with optimized HF buffers suit custom reaction optimization, particularly for difficult templates requiring buffer additive titration. In contrast, 2x master mixes streamline routine high-fidelity amplification for cloning and mutagenesis workflows.
Error Rate Requirements
For applications where amplicons will be cloned and sequence-verified downstream, the approximately 50- to 100-fold lower error rate of high-fidelity enzymes compared with standard Taq polymerase substantially reduces the frequency of sequence-confirmed clones carrying PCR-introduced mutations.
Extension Speed and Cycling Time
High-fidelity polymerases often extend faster than standard Taq per kilobase, which can shorten overall cycling time for long amplicons. However, optimal cycling parameters are enzyme-specific and should follow the manufacturer's recommendations.
Cloning Method Compatibility
Some high-fidelity polymerases generate blunt-ended PCR products suited to Gibson Assembly or blunt-end cloning strategies, while others may require additional steps for TA cloning. Confirm compatibility with your intended downstream cloning method before selection.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The development of high-fidelity thermostable DNA polymerases&mdash;combining thermostability with 3' to 5' proofreading exonuclease activity&mdash;transformed PCR from a detection and amplification tool into a precise DNA engineering platform, enabling reliable cloning, site-directed mutagenesis, and sequencing-grade amplification. The proofreading mechanism corrects misincorporated bases immediately after insertion, reducing error rates to approximately 10&minus;6 per nucleotide. Selection between high-fidelity polymerases often depends on template characteristics such as GC content, amplicon length, secondary structure, and downstream cloning requirements. High-fidelity enzymes are typically stored at &minus;20 &deg;C with matched HF buffer systems, and lot-specific Certificates of Analysis (CoAs) are available upon request. For sequencing-bound amplicons, confirm that the selected polymerase's documented error rate meets the level of sequence accuracy required, as even a 50- to 100-fold improvement over standard Taq may not be sufficient for applications demanding extremely low mutation rates across very long amplicons. Product availability reflects MBP's catalogue as of mid-2026.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP </a>today to find high-fidelity DNA polymerase solutions for streamlining your laboratory workflows.

ExtremeTaq DNA Polymerase - Product Sheet

ExtremeTaq™ DNA Polymerase (With 5x Reaction Buffer)

ExtremeTaq™ DNA Polymerase is available in the following variants: AZ-1905, AZ-1915, AZ-1950.ExtremeTaq™ DNA Polymerase (AZ-1905) by Azura Genomics is a 5 U/µL hot-start, proofreading-enhanced Taq formulation delivering up to 10× higher fidelity than standard Taq and long PCR up to 10 kb. Supplied with 5× buffer in 500 U, 1,000 U, and 5,000 U sizes from MBP Inc.

ExtremeTaq™ DNA Polymerase (With 5x Reaction Buffer), 500 U (5U/μL)

ExtremeTaq™ DNA Polymerase (With 5x Reaction Buffer), 1000 U (5U/μL)

ExtremeTaq™ DNA Polymerase (With 5x Reaction Buffer), 5000 U (5U/μL)

High-Fidelity Master Mix

<h2 dir="ltr">What Is a High-Fidelity PCR Master Mix?</h2>
 A high-fidelity PCR master mix is a 2x concentrated, pre-formulated solution containing a proofreading DNA polymerase (Phusion, Q5, KOD, or Pfu-equivalent), dNTPs, MgCl2, and reaction buffer &mdash; requiring only the addition of template and primers to set up a reaction. The proofreading polymerase's 3' to 5' exonuclease activity corrects misincorporated nucleotides during synthesis, reducing the error rate roughly 50-100-fold compared to standard Taq master mixes. Choose a high-fidelity master mix whenever the amplicon will be cloned, mutagenised, or sequenced &mdash; not for routine genotyping, where standard Taq is sufficient and lower cost.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="uyjmeg" data-start="418" data-end="539">Streamlined post-amplification analysis with high-fidelity 2X red mix formulations with direct gel-loading capability</li>
<li data-section-id="1rh0fvx" data-start="540" data-end="630">Pre-optimized high-fidelity 2X PCR master mixes containing proofreading DNA polymerase</li>
<li data-section-id="6drhog" data-start="631" data-end="735">Reduced non-specific amplification and primer-dimer formation compared to standard Taq-based systems</li>
<li data-section-id="1pa7u4r" data-start="736" data-end="826">High-fidelity amplification suitable for cloning workflows requiring sequence accuracy</li>
<li data-section-id="1n3ow6j" data-start="827" data-end="925">2X master mix formats designed for reproducible amplification across a range of template types</li>
</ul>
<h2 dir="ltr"> How to Choose a High-Fidelity Master Mix</h2>
 Downstream Application Cloning into expression vectors, site-directed mutagenesis, and sequencing-bound amplicons all benefit from the reduced error rate of a high-fidelity master mix. Routine genotyping or colony screening does not require this level of accuracy and is typically served by standard Taq master mixes at a lower cost. Template Difficulty (GC Content, Length) Some high-fidelity master mix formulations are specifically optimised for GC-rich templates or long amplicons (&gt;5 kb), where standard high-fidelity formulations may show reduced yield. Check product-specific template compatibility notes before ordering for difficult targets. Extension Time and Cycling Protocol High-fidelity polymerases often have different optimal extension times per kilobase compared to standard Taq &mdash; some extend faster, shortening overall cycling time for long amplicons. Follow the manufacturer's recommended cycling parameters rather than reusing a standard Taq protocol. Cloning Method Compatibility Some high-fidelity master mixes produce blunt-ended products suited to Gibson Assembly or blunt cloning strategies; others may need additional A-tailing for TA cloning. Confirm compatibility with your intended downstream cloning method before selecting a product. Format Convenience vs. Component Flexibility The 2x master mix format minimises pipetting steps and improves reproducibility for routine high-fidelity workflows. Researchers needing to titrate individual components (e.g., additional MgCl2 for a specific template) may prefer the individual high-fidelity enzyme plus separate buffer instead. 
<h2 dir="ltr"> Specifications Context</h2>
 A 2x high-fidelity master mix is set up identically to a standard 2x Taq master mix &mdash; combined with template and primers at the manufacturer-specified ratio to reach 1x working concentration &mdash; but the included proofreading polymerase detects and removes misincorporated bases immediately after insertion, reducing the per-cycle error rate to approximately 10⁻⁶ per nucleotide. This level of accuracy matters specifically because any error introduced during amplification is propagated into the final cloned construct or sequencing read. High-fidelity master mixes are typically stored at &minus;20 &deg;C; lot-specific CoAs documenting activity and quality control are available on request. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get pre-optimized High-Fidelity Master Mix solutions for your lab.

ExtremeTaq HiFi Red Mix - Product Sheet

ExtremeTaq™ HiFi Red Mix

ExtremeTaq™ HiFi Red Mix is available in the following variants: AZ-1910, AZ-1911, AZ-1912.AZ-1910 ExtremeTaq™ HiFi Red Mix by Azura Genomics is a 2x hot-start PCR master mix with up to 10x fidelity vs Taq, antibody-mediated hot-start, proofreading 3'→5' exonuclease, direct-load red dye, 10 kb amplicon capacity, available in 200–8000 reaction pack sizes.

ExtremeTaq HiFi Red Mix 200 Reactions

ExtremeTaq HiFi Red Mix 1000 Reactions

ExtremeTaq HiFi Red Mix 2000 Reactions

Lytic Enzymes

<h2>What Are Lytic Enzymes?</h2>
&nbsp;
Lytic enzymes degrade the structural polysaccharide scaffolding of microbial cell walls, enabling cell disruption under mild conditions without mechanical force. Lysozyme (N-acetylmuramide glycanohydrolase) cleaves the beta-1,4 glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine residues in bacterial peptidoglycan, lysing gram-positive bacteria efficiently and gram-negative bacteria when paired with EDTA to destabilize the outer membrane. Lyticase from Arthrobacter luteus hydrolyzes beta-1,3-glucan in yeast cell walls and is the standard reagent for generating spheroplasts from Saccharomyces cerevisiae and other yeast species. Lysostaphin is a glycyl-glycine endopeptidase that specifically cleaves the polyglycine cross-links in Staphylococcus aureus peptidoglycan. Mutanolysin cleaves beta-1,4 linkages in the alternating N-acetylmuramyl-N-acetylglucosamine backbone of gram-positive bacteria, complementing lysozyme for complete lysis of resistant species. Choose the lytic enzyme based on organism type (bacterial vs. yeast/fungal), Gram stain classification, and intended downstream workflow (DNA extraction, protein isolation, or transformation).
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Zymolyase&trade; Ultra: An elite, high-activity enzyme optimized for rapid fungal cell wall digestion and maximum nucleic acid recovery.
</li>
<li dir="ltr" aria-level="1">
Flash-Extract Lysis Solution: A streamlined reagent for immediate genomic DNA release from diverse samples, ideal for NGS integration.
</li>
<li dir="ltr" aria-level="1">
Zymolyase&trade; w/ Storage Buffer: A stabilized formulation designed to maintain long-term enzymatic potency and consistent performance across all assays.
</li>
</ul>
&nbsp;
<h2>How to Choose Lytic Enzymes</h2>
&nbsp;
Gram-positive vs. gram-negative bacteria
Lysozyme alone lyses most gram-positive bacteria efficiently at concentrations of 0.1-1 mg/mL in 10 mM Tris-HCl, pH 8.0, 1 mM EDTA at 37 degrees C for 30-60 minutes. Gram-negative bacteria require EDTA (typically 10-50 mM) to chelate divalent cations and disrupt the outer membrane before lysozyme can access the peptidoglycan. Species with thick, atypical cell walls, such as Micrococcus, Staphylococcus, and Streptococcus, may respond better to lysostaphin, mutanolysin, or mechanical disruption combined with enzymatic lysis.
Yeast and fungal lysis
Lyticase (minimum 2,000 U/mg protein) is the reagent of choice for yeast spheroplast generation and DNA/RNA extraction from Saccharomyces, Candida, Pichia, Kluyveromyces, and related species. A typical protocol uses 10-100 U of lyticase per OD600 unit of yeast cells in 1 M sorbitol buffer at 30 degrees C for 30-60 minutes. Kitalase (from Rhizoctonia solani) combines beta-1,3-glucanase with protease, pectinase, and amylase activity, providing broader lytic activity for resistant fungal species.
DNA purity requirements
For microbiome research and 16S/18S rDNA amplification, lytic enzymes must be certified free of contaminating DNA. Lyticase lots used in microbiome studies are QC-tested by 35-cycle PCR amplification of 16S and 18S rDNA using universal primer sets; confirm this certification with each lot before use in amplicon sequencing workflows.
Downstream compatibility
Most lytic enzymes are inactivated by subsequent SDS treatment or heating to 95 degrees C for 5 minutes, making them compatible with standard nucleic acid extraction kits. Lyticase requires removal before silica column binding; heat inactivation at 70 degrees C for 10 minutes or proteinase K digestion at 55 degrees C for 30 minutes accomplishes this. Confirm inactivation conditions in the manufacturer's protocol before proceeding.
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<h2>Specifications Context</h2>
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Lysozyme activity is measured in units per milligram of protein, where 1 unit is defined as the decrease in absorbance of 0.001 per minute at 450 nm when Micrococcus lysodeikticus cells are used as substrate at pH 6.24 and 25 degrees C. Lyticase activity is expressed as units per milligram protein; 1 unit releases 1 nmol of glucose from yeast cell walls per minute at 30 degrees C. Lysozyme is stable for 12 months at 4 degrees C as a lyophilized powder and should be dissolved in 10 mM sodium acetate, pH 4.5-5.0, for storage in solution. Lytic enzyme combinations with mechanical disruption (bead beating) are standard for complete lysis of difficult samples, including Mycobacterium, spore-forming bacteria, and thick-walled fungi in clinical and microbiome research workflows. For downstream removal of protein from lysates, see <a href="https://mbpinc.net/reagents/molecular-biology/enzymes-and-inhibitors/proteases">proteases,</a> including Proteinase K, for nucleic acid cleanup.
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Streamline your path to discovery&mdash;<a href="https://mbpinc.net/contact-us">contact the MBP team</a> today to secure a quote for our advanced lytic reagents.

Flash-Extract Lysis Solution

Azura Genomics Flash-Extract Lysis Solution: 8-min single-tube lysis of mouse tails, ear punches, plant tissue, bacteria, and saliva — PCR-ready DNA in under 60 min total. AZ-3410 (100 rxns, 8×1.25 mL) and AZ-3440 (400 rxns, 8×5 mL).

Flash-Extract Lysis Solution 100 reactions

Flash-Extract Lysis Solution 400 reactions

Flash-Extract PCR Kit - Product Sheet

Flash-Extract™ PCR Kit

The Flash-Extract™ PCR Kit is available in the following variants: AZ-3310, AZ-3340.AZ-3310 by Azura Genomics is the Flash-Extract™ PCR Kit: integrated DNA extraction and PCR amplification from mouse tissue, plant, bacteria, and saliva in under 60 minutes. No centrifugation required. Available in 100-, 400-, and 2000-reaction packs.

Flash-Extract PCR Kit 100 reactions

Flash-Extract PCR Kit 400 reactions

Reverse Transcriptase

<h2>What Is Reverse Transcriptase?</h2>
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Reverse transcriptase is an RNA-dependent DNA polymerase that synthesizes a complementary DNA (cDNA) strand using an RNA template, providing the essential first step for RT-PCR, RT-qPCR, cDNA library construction, and gene expression analysis. Reverse transcriptases differ in thermostability, processivity, RNase H activity, and compatibility with challenging RNA templates. Selecting the appropriate enzyme depends on your RNA quality, transcript length, and downstream application.
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<h2>What you will find:</h2>
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<ul>
<li>Reverse transcriptase-based systems designed to support cDNA synthesis across a range of RNA templates, including workflows involving structured or challenging RNA.</li>
<li>One-step RT-PCR and RT-qPCR kits featuring enzyme systems designed to reduce non-specific amplification through optimized reaction setup and enzyme activation strategies.</li>
<li>High-efficiency reverse transcription and RT-PCR chemistries suitable for low-abundance RNA inputs and sensitive gene expression analysis.</li>
<li>cDNA synthesis and RT-PCR systems supporting a range of transcript lengths depending on enzyme selection and reaction conditions.</li>
<li>Thermostable and high-performance reverse transcription systems designed to improve cDNA yield and workflow reliability across RNA applications.</li>
</ul>
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<h2>How to Choose a Reverse Transcriptase</h2>
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Enzyme Selection
Reverse transcriptases differ in thermostability, RNase H activity, processivity, and compatibility with different RNA templates. Select an enzyme that matches your RNA quality, transcript length, and downstream application requirements.
RNase H Activity
Reverse transcriptases with reduced RNase H activity minimize RNA template degradation during cDNA synthesis, helping improve the yield of longer cDNA products for applications such as full-length transcript analysis and cDNA library preparation.
Thermostability
Thermostable reverse transcriptases can operate at higher reaction temperatures, helping reduce RNA secondary structure and improving cDNA synthesis from GC-rich or highly structured RNA templates.
Template Length and Application
Choose an enzyme validated for your intended transcript length and downstream workflow, whether performing routine RT-PCR, quantitative RT-qPCR, full-length cDNA synthesis, or library preparation.
Workflow Compatibility
Consider whether a stand-alone reverse transcriptase or an integrated one-step RT-PCR system best fits your workflow, throughput requirements, and downstream amplification strategy.
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<h2>Specifications Context</h2>
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Reverse transcriptase enzymes catalyze the synthesis of complementary DNA (cDNA) from RNA templates, enabling downstream PCR- and qPCR-based analysis of gene expression. Modern reverse transcriptases are available with varying levels of thermostability, processivity, and RNase H activity to support applications ranging from routine first-strand cDNA synthesis to amplification of structured or low-abundance RNA targets. Most enzymes are supplied for storage at &minus;20&nbsp;&deg;C, and RNase-free handling practices are recommended throughout RNA workflows to preserve sample integrity and maximize cDNA yield.
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get research-grade reverse transcription enzymes for your lab.

HighTherm Reverse Transcriptase - Product Sheet

HighTherm™ Reverse Transcriptase

HighTherm™ Reverse Transcriptase is available in the following variants: AZ-1991, AZ-1994.AZ-1991 by Azura Genomics: HighTherm™ Reverse Transcriptase — engineered MMLV RT with &gt;90% activity at 50°C/60 min. Working range 38–55°C. Synthesizes 100 bp–&gt;12 kb cDNA from 1 pg–5 µg total RNA. Reduced RNase H activity for full-length cDNA. Supplied at 200 U/µl with 5× Buffer.