Name: HighTherm™ Reverse Transcriptase
SKU: AZ-1991
Availability: InStock

Reverse Transcriptase

<h2>What Is Reverse Transcriptase?</h2>
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Reverse transcriptase is an RNA-dependent DNA polymerase that synthesizes a complementary DNA (cDNA) strand using an RNA template, providing the essential first step for RT-PCR, RT-qPCR, cDNA library construction, and gene expression analysis. Reverse transcriptases differ in thermostability, processivity, RNase H activity, and compatibility with challenging RNA templates. Selecting the appropriate enzyme depends on your RNA quality, transcript length, and downstream application.
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<h2>What you will find:</h2>
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<ul>
<li>Reverse transcriptase-based systems designed to support cDNA synthesis across a range of RNA templates, including workflows involving structured or challenging RNA.</li>
<li>One-step RT-PCR and RT-qPCR kits featuring enzyme systems designed to reduce non-specific amplification through optimized reaction setup and enzyme activation strategies.</li>
<li>High-efficiency reverse transcription and RT-PCR chemistries suitable for low-abundance RNA inputs and sensitive gene expression analysis.</li>
<li>cDNA synthesis and RT-PCR systems supporting a range of transcript lengths depending on enzyme selection and reaction conditions.</li>
<li>Thermostable and high-performance reverse transcription systems designed to improve cDNA yield and workflow reliability across RNA applications.</li>
</ul>
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<h2>How to Choose a Reverse Transcriptase</h2>
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Enzyme Selection
Reverse transcriptases differ in thermostability, RNase H activity, processivity, and compatibility with different RNA templates. Select an enzyme that matches your RNA quality, transcript length, and downstream application requirements.
RNase H Activity
Reverse transcriptases with reduced RNase H activity minimize RNA template degradation during cDNA synthesis, helping improve the yield of longer cDNA products for applications such as full-length transcript analysis and cDNA library preparation.
Thermostability
Thermostable reverse transcriptases can operate at higher reaction temperatures, helping reduce RNA secondary structure and improving cDNA synthesis from GC-rich or highly structured RNA templates.
Template Length and Application
Choose an enzyme validated for your intended transcript length and downstream workflow, whether performing routine RT-PCR, quantitative RT-qPCR, full-length cDNA synthesis, or library preparation.
Workflow Compatibility
Consider whether a stand-alone reverse transcriptase or an integrated one-step RT-PCR system best fits your workflow, throughput requirements, and downstream amplification strategy.
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<h2>Specifications Context</h2>
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Reverse transcriptase enzymes catalyze the synthesis of complementary DNA (cDNA) from RNA templates, enabling downstream PCR- and qPCR-based analysis of gene expression. Modern reverse transcriptases are available with varying levels of thermostability, processivity, and RNase H activity to support applications ranging from routine first-strand cDNA synthesis to amplification of structured or low-abundance RNA targets. Most enzymes are supplied for storage at &minus;20&nbsp;&deg;C, and RNase-free handling practices are recommended throughout RNA workflows to preserve sample integrity and maximize cDNA yield.
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get research-grade reverse transcription enzymes for your lab.

User Manual

HighTherm Reverse Transcriptase - Product Sheet

MSDS

HighTherm™ Reverse Transcriptase

HighTherm™ Reverse Transcriptase is available in the following variants: AZ-1991, AZ-1994.AZ-1991 by Azura Genomics: HighTherm™ Reverse Transcriptase — engineered MMLV RT with &gt;90% activity at 50°C/60 min. Working range 38–55°C. Synthesizes 100 bp–&gt;12 kb cDNA from 1 pg–5 µg total RNA. Reduced RNase H activity for full-length cDNA. Supplied at 200 U/µl with 5× Buffer.