Azura Reagents

Taq PCR Master Mixes

<h2 dir="ltr"> What Is a Taq PCR Master Mix?</h2>
 The 2x Taq PCR master mix format has become the standard for high-throughput molecular biology workflows because it combines reliability, convenience, and reproducibility in a single-tube format. By pre-optimising the concentrations of all PCR components, master mixes reduce pipetting steps and experimental variability compared to assembling reactions from individual components. This simplifies workflow setup and improves reproducibility. Select a formulation designed for your amplification needs.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr">High-Purity Enzymes: Versatile independent polymerases, such as the Azura&trade; and Accuris series, crafted for adaptable reaction adjustments and enhanced processivity with various DNA templates.</li>
<li dir="ltr">Full Reaction Solutions: All-inclusive systems such as the PCR Sure&trade; Kit that feature optimized dNTPs and buffers, offering a ready-to-use option for labs requiring dependable high-throughput performance and consistent outcomes.</li>
</ul>
<h2 dir="ltr"> How to Choose a Taq PCR Master Mix</h2>
 Standard vs. Dye-Containing Standard 2x master mixes require a separate loading dye addition before gel electrophoresis. Dye-containing master mixes include loading dye in the formulation, allowing direct gel loading after amplification &mdash; streamlining routine band-confirmation workflows. Component Pre-Optimisation A 2x Taq PCR master mix is a pre-formulated, double-concentrated solution containing Taq DNA polymerase, dNTPs (typically 200 &micro;M each at 1x), MgCl2, and PCR buffer at optimised concentrations &mdash; requiring only template and primer addition to set up a reaction. Throughput Scale High-throughput genotyping and colony screening benefit most from master mix consistency across multi-well plates, where individual component pipetting would introduce variability and time cost. Storage and Stability Master mixes are typically stable at &minus;20 &deg;C with multiple freeze-thaw cycles, though some dye-containing formulations may have slightly different stability profiles &mdash; check product-specific storage recommendations. Compatibility with Existing Protocols When switching from individual-component reactions to a master mix format, confirm that the pre-set MgCl2 and dNTP concentrations are compatible with your existing primer designs and templates, or adjust accordingly. 
<h2 dir="ltr"> Specifications Context</h2>
 A 2x Taq PCR master mix is set up by combining the master mix with template DNA and primers &mdash; typically at a 1:1 ratio of master mix to combined template/primer/water volume, achieving the final 1x working concentration. This format has become the standard for high-throughput molecular biology because it reduces pipetting steps and lot-to-lot variability across multi-well plate setups, important for reproducibility in genotyping and screening pipelines. Master mixes are typically stored at &minus;20 &deg;C with documented multiple freeze-thaw tolerance. When transitioning a protocol from individual-component PCR to a master mix format, running both formulations side-by-side on the same template and primer set is a useful validation step, confirming that the pre-set MgCl2 and dNTP concentrations in the master mix produce comparable yield and specificity to the previously optimised individual-component reaction before fully adopting the new format across a workflow. Product availability reflects MBP's catalogue as of mid-2026. 
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Enhance your amplification outcomes&mdash;<a href="https://mbpinc.net/contact-us">reach out to the MBP team </a>now for a quote on our expert Taq Polymerase offerings.

User Manual

Azura 2x Taq Mix - Product Sheet

MSDS

Azura Taq Master Mix

Azura Taq Master Mix is available in the following variants: AZ-1302, AZ-1310, AZ-1320.AZ-1302 by Azura Genomics is a 2x ready-to-use Taq DNA polymerase master mix containing Taq polymerase, a proprietary PCR enhancer, and pre-optimized dNTPs and MgCl2. Formulated for high-throughput PCR, genotyping, colony PCR, and TA cloning. Available in 200-reaction (4×1.25 mL), 1000-reaction, and 2000-reaction formats.

Manufacturer

Azura 2x Taq Mix 200 reactions

Azura 2x Taq Mix 1000 reactions

Azura 2x Taq Mix 2000 reactions

High-Fidelity DNA Polymerase

<h2>What Are High-Fidelity DNA Polymerases?</h2>
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The development of high-fidelity thermostable DNA polymerases transformed PCR from a tool for detection and amplification into a precise DNA engineering platform. By combining thermostability with 3' to 5' proofreading exonuclease activity, modern high-fidelity enzymes detect and remove misincorporated bases immediately after insertion during DNA synthesis. Polymerase fidelity can significantly affect downstream results, so choosing an enzyme that meets your accuracy and performance requirements is essential.
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<h2>What you will find:</h2>
&nbsp;
<ul>
<li>High-fidelity DNA amplification with proofreading activity using Azura TruFi&trade; II DNA Polymerase.</li>
<li>Proofreading polymerase formulations with matched reaction buffers, including ExtremeTaq&trade; DNA Polymerase with 5x Reaction Buffer.</li>
<li>Accurate PCR amplification with Accuris High Fidelity DNA Polymerase for cloning, sequencing, and mutagenesis workflows.</li>
<li>High-fidelity enzyme systems featuring proofreading activity, including MegaFi Pro Fidelity DNA Polymerase.</li>
</ul>
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<h2>How to Choose a High-Fidelity DNA Polymerase</h2>
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Enzyme Family Selection
High-fidelity polymerases differ in processivity, extension speed, fidelity, and template compatibility. Selection should consider amplicon length, GC content, template complexity, and downstream application requirements.
Format: Enzyme vs. Master Mix
Individual high-fidelity enzymes with optimized HF buffers suit custom reaction optimization, particularly for difficult templates requiring buffer additive titration. In contrast, 2x master mixes streamline routine high-fidelity amplification for cloning and mutagenesis workflows.
Error Rate Requirements
For applications where amplicons will be cloned and sequence-verified downstream, the approximately 50- to 100-fold lower error rate of high-fidelity enzymes compared with standard Taq polymerase substantially reduces the frequency of sequence-confirmed clones carrying PCR-introduced mutations.
Extension Speed and Cycling Time
High-fidelity polymerases often extend faster than standard Taq per kilobase, which can shorten overall cycling time for long amplicons. However, optimal cycling parameters are enzyme-specific and should follow the manufacturer's recommendations.
Cloning Method Compatibility
Some high-fidelity polymerases generate blunt-ended PCR products suited to Gibson Assembly or blunt-end cloning strategies, while others may require additional steps for TA cloning. Confirm compatibility with your intended downstream cloning method before selection.
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<h2>Specifications Context</h2>
&nbsp;
The development of high-fidelity thermostable DNA polymerases&mdash;combining thermostability with 3' to 5' proofreading exonuclease activity&mdash;transformed PCR from a detection and amplification tool into a precise DNA engineering platform, enabling reliable cloning, site-directed mutagenesis, and sequencing-grade amplification. The proofreading mechanism corrects misincorporated bases immediately after insertion, reducing error rates to approximately 10&minus;6 per nucleotide. Selection between high-fidelity polymerases often depends on template characteristics such as GC content, amplicon length, secondary structure, and downstream cloning requirements. High-fidelity enzymes are typically stored at &minus;20 &deg;C with matched HF buffer systems, and lot-specific Certificates of Analysis (CoAs) are available upon request. For sequencing-bound amplicons, confirm that the selected polymerase's documented error rate meets the level of sequence accuracy required, as even a 50- to 100-fold improvement over standard Taq may not be sufficient for applications demanding extremely low mutation rates across very long amplicons. Product availability reflects MBP's catalogue as of mid-2026.
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<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP </a>today to find high-fidelity DNA polymerase solutions for streamlining your laboratory workflows.

TruFi II DNA Polymerase - Product Sheet

Azura TruFi™ II DNA Polymerase

Azura Genomics Azura TruFi™ II DNA Polymerase: 2 U/µL high-fidelity proofreading polymerase, error rate 4.55×10⁻⁷ (~50× vs Taq), ≤10 kb amplicons. 5× buffer with GC enhancers/dNTPs/MgCl₂ included. AZ-1702 (200 U) and AZ-1710 (1,000 U).

Azura TruFi DNA Polymerase 200 units

Azura TruFi DNA Polymerase 1,000 units

cDNA Synthesis Kits

<h2 dir="ltr"> What is a cDNA Synthesis Kit?</h2>
 A cDNA synthesis kit provides the complete set of reagents - reverse transcriptase enzyme, reaction buffer, dNTPs, RNase inhibitor, and primers - needed to perform first-strand reverse transcription, converting an RNA sample into a cDNA pool. Unlike one-step RT-PCR kits, which combine RT and PCR in a single reaction with gene-specific primers, a cDNA synthesis kit performs reverse transcription as a standalone step, producing a cDNA template that can be used across multiple subsequent PCR or qPCR reactions targeting different genes. Choose a cDNA synthesis kit when one RNA sample needs to be analysed for multiple gene targets, or when downstream qPCR requires a separately-generated, quality-checked cDNA template. 
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li data-section-id="1o7fvd2" data-start="74" data-end="228">First-strand cDNA synthesis kits for RNA-to-cDNA conversion in gene expression workflows, including AzuraQuant&trade; and AzuraFlex&trade; cDNA synthesis kits</li>
<li data-section-id="z40sk0" data-start="229" data-end="395">Standard cDNA synthesis kits for routine transcriptional analysis and downstream PCR applications, including Accuris qMAX and 1st cDNA Synthesis Kit (FS01-00)</li>
<li data-section-id="1yvybqf" data-start="396" data-end="521">Flexible and high-efficiency cDNA synthesis systems designed to support a range of RNA inputs and experimental conditions</li>
<li data-section-id="sl2teb" data-start="522" data-end="599">miRNA-specific cDNA synthesis solutions for small RNA expression analysis</li>
<li data-section-id="17gz9kt" data-start="600" data-end="770" data-is-last-node="">One-step and integrated RT-based systems and master mixes supporting streamlined reverse transcription workflows, including OneScript Plus and OneScript Hot RT kits</li>
</ul>
<h2 dir="ltr"> How to Choose a cDNA Synthesis Kit</h2>
 Priming Strategy Oligo(dT) primers target the poly-A tail of mRNA, enriching specifically for mRNA-derived cDNA and reducing genomic DNA or rRNA background. Random primers (commonly hexamers) prime throughout the RNA template regardless of sequence, useful for templates lacking a poly-A tail (such as bacterial RNA) or when full-length cDNA coverage, including 5' regions, is needed. Some kits offer a blend of both primer types for balanced coverage. Reverse Transcriptase Included Kits built around M-MLV RNase H-minus reverse transcriptase favour longer, more complete cDNA products, while kits using enhanced-thermostability or inhibitor-resistant reverse transcriptases may better handle GC-rich templates or minimally purified RNA samples. Genomic DNA Elimination Step Some cDNA synthesis kits include a separate genomic DNA elimination (gDNA wipeout) step performed before reverse transcription -- important for accurate gene expression quantification, since residual genomic DNA can contribute false signal in downstream qPCR if primers span regions present in both genomic DNA and the cDNA target. Reaction Volume and Throughput cDNA synthesis kits are available in formats scaled for individual reactions through to high-throughput 96-well formats, allowing labs to match kit format to typical sample batch sizes without excessive reagent waste. Downstream Application Compatibility The resulting cDNA pool should be compatible with the intended downstream application -- standard PCR, qPCR with SYBR Green or probe-based detection, or digital PCR -- confirm that the kit's buffer system does not interfere with the chosen downstream detection chemistry. 
<h2 dir="ltr"> Specifications Context</h2>
 In a two-step workflow, the first step synthesises first-strand cDNA using a reverse transcriptase such as M-MLV-RT with the chosen priming strategy, and the resulting cDNA pool can then be used in multiple separate PCR or qPCR reactions targeting different genes - a key advantage when a single RNA sample needs to be analysed for several targets, since the cDNA synthesis step is performed only once. Genomic DNA elimination steps, where included, are typically performed as a brief pre-treatment with a DNase before the reverse transcription reaction itself begins. cDNA synthesis kit components - enzyme, buffer, dNTPs, and RNase inhibitor - are typically stored at &minus;20 &deg;C; RNase-free water and RNase inhibitor handling throughout the workflow are recommended to prevent RNA template degradation before synthesis.
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get high-precision cDNA synthesis kits for your lab.

Product Literature

AzuraFlex™ cDNA Synthesis Kit

AZ-1997 AzuraFlex cDNA Synthesis Kit by Azura Genomics converts 10 pg–2 µg total RNA into cDNA (up to 9 kb) using MMLV-derived reverse transcriptase with random hexamer and oligo(dT) primers. 20 µl reactions; −20°C storage.

AzuraFlex cDNA Synthesis Kit 200 Reactions

AzuraFlex cDNA Synthesis Kit 1000 Reactions

SYBR/Primer-Probe

<h2 dir="ltr"> What Is a SYBR/Primer-Probe Master Mix?</h2>
 A SYBR/Primer-Probe master mix is a 2x formulation positioned to support both dye-based (SYBR Green) and primer-probe (hydrolysis probe, TaqMan-style) detection chemistries, typically built on a common hot-start Taq, dNTP, MgCl2, and buffer backbone with formulation adjustments that accommodate either an intercalating dye or a labelled probe, depending on the assay. Choose this combined format when your lab runs both SYBR Green and probe-based assays and wants to standardise on fewer master mix products across the qPCR workflow.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="tosb50" data-start="110" data-end="247">One-step RT-qPCR kits that combine reverse transcription and real-time PCR in a single tube to streamline RNA quantification workflows.</li>
<li data-section-id="11s385w" data-start="248" data-end="363">Hot-start enzyme systems are designed to reduce non-specific amplification during reaction setup and thermal cycling.</li>
<li data-section-id="1sl7e5s" data-start="364" data-end="494">Probe-based detection chemistries for specific RNA target quantification in gene expression and pathogen detection applications.</li>
<li data-section-id="7bdacc" data-start="495" data-end="613">HiROX, LoROX, and NoROX formulation options to support compatibility with a wide range of real-time PCR instruments.</li>
<li data-section-id="19ytf2e" data-start="614" data-end="737">Optimized one-step workflows that reduce sample handling and help minimize contamination risk in high-throughput studies.</li>
</ul>
<h2 dir="ltr"> How to Choose a SYBR/Primer-Probe Master Mix</h2>
 Assay Portfolio Mix Labs running a mix of SYBR Green gene expression assays and probe-based genotyping or pathogen-detection assays can benefit from standardising on a combined-compatible master mix line, reducing the number of distinct products to validate, store, and track across the facility. Reference Dye Configuration Confirm whether the specific product includes ROX or other passive reference dyes as standard, since probe-based assays in particular often rely on ROX for instrument normalisation, while some SYBR-only workflows may use ROX-free instruments. Validation Across Chemistries When adopting a combined-compatible master mix for the first time, validating performance separately for both a representative SYBR Green assay and a representative probe-based assay is recommended, since buffer optimisations that favour one chemistry slightly can sometimes trade off against the other. Primer and Probe Concentration Guidelines Typical primer-probe qPCR setups use primer pairs at a final concentration around 0.5 &micro;M and probes at 0.25-0.5 &micro;M; confirm the master mix manufacturer's recommended concentration ranges for both components when switching from a single-chemistry product. Throughput and Inventory Simplification For core facilities supporting multiple research groups with different assay types, a combined-compatible master mix can simplify inventory management and reduce the risk of using the wrong master mix for a given assay type. 
<h2 dir="ltr"> Specifications Context</h2>
 Standard real-time PCR master mixes are 2x concentrated and contain dNTPs, MgCl2, and DNA polymerase as their common backbone; a SYBR/primer-probe combined format extends this backbone with formulation choices intended to perform acceptably whether the researcher adds SYBR Green dye for dye-based detection or a labelled probe for probe-based detection. In probe-based setups, primer pairs are commonly used at a final concentration around 0.5 &micro;M with probes at 0.25-0.5 &micro;M, while SYBR-based protocols may additionally include a small amount of random hexamer primer in RT-qPCR contexts. Real-time PCR master mixes of this type are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light; lot-specific CoAs are available on request. 
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get high-yield&nbsp;SYBR/Primer-Probe Master Mixes for your lab.

AzuraQuant Probe 1-Step qPCR Mix - Product Sheet

AzuraQuant Probe 1-Step qPCR Mix HiRox

The AzuraQuant Probe 1-Step qPCR Mix HiRox by Azura Genomics is a 2× one-step RT-qPCR master mix with high ROX reference dye, compatible with TaqMan®, Scorpions®, and molecular beacon probes; 1 pg–1 µg RNA input; 20 µl reactions; stored at −20°C. Available in 100 rxns (AZ-3901), 500 rxns (AZ-3905), 1000 rxns (AZ-3910), and 5000 rxns (AZ-3950).

AzuraQuant Probe 1-Step qRT-PCR Kit HiRox 100 reactions

AzuraQuant Probe 1-Step qRT-PCR Kit HiRox 500 reactions

AzuraQuant Probe 1-Step qRT-PCR Kit HiRox 1,000 reactions

AzuraQuant Probe 1-Step qRT-PCR Kit HiRox 5,000 reactions

AzuraQuant Probe 1-Step qPCR Mix LoRox

The AzuraQuant Probe 1-Step qPCR Mix LoRox by Azura Genomics is a 2× hot-start RT-qPCR master mix with 20× AzuraSprint Reverse Transcriptase, compatible with TaqMan, Scorpions, and molecular beacon probes; LoRox formulation for low passive reference instruments. Available in four sizes: AZ-4101 (100 rxns), AZ-4105 (500 rxns), AZ-4110 (1,000 rxns), and AZ-4150 (5,000 rxns).

AzuraQuant Probe 1-Step qRT-PCR Kit LoRox 100 reactions

AzuraQuant Probe 1-Step qRT-PCR Kit LoRox 500 reactions

AzuraQuant Probe One-Step qPCR Mix LoRox 1,000 Reactions

AzuraQuant Probe One-Step qPCR Mix LoRox 5,000 Reactions

AzuraQuant Probe 1-Step qPCR Mix NoRox

Azura Genomics AzuraQuant™ Probe 1-Step qPCR Mix NoRox: 2× RT-qPCR master mix (Azura HS Taq + AzuraSprint RT), TaqMan/Scorpions/molecular beacon compatible, no ROX. Four sizes: AZ-4301 (100), AZ-4305 (500), AZ-4310 (1,000), AZ-4350 (5,000 rxns).

AzuraQuant Probe 1-Step qPCR Mix No-Rox 100 Reactions

AzuraQuant Probe 1-Step qPCR Mix No-Rox 500 Reactions

AzuraQuant Probe 1-Step qRT-PCR Kit NoRox 1,000 reactions

AzuraQuant Probe 1-Step qRT-PCR Kit NoRox 5,000 reactions

Primer-Probe

<h2 dir="ltr"> What Is a Primer-Probe Master Mix?</h2>
 A primer-probe master mix is a 2x concentrated formulation built around a hot-start Taq DNA polymerase, dNTPs, MgCl2, and optimised buffer, designed for use with hydrolysis-probe detection chemistries such as TaqMan. Unlike SYBR Green dye-based mixes, primer-probe formulations rely on a separately added, fluorescently labelled, sequence-specific probe combined with target-specific primers to generate the detection signal, with specificity coming from both primer and probe sequence matching. Choose a primer-probe master mix when assay specificity is critical, when multiplexing several targets in one well is needed, or when working with an established probe-based assay design.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="qhwnhl" data-start="279" data-end="413">Probe-based qPCR master mixes with HiROX, LoROX, and ROX-free options for compatibility across a range of real-time PCR instruments.</li>
<li data-section-id="1cnihmh" data-start="414" data-end="543">Hot-start polymerase formulations designed to support specific target detection in hydrolysis probe (TaqMan-style) qPCR assays.</li>
<li data-section-id="51tf3n" data-start="544" data-end="643">Blue tracking dye probe master mixes for easier reaction setup and visual pipetting confirmation.</li>
<li data-section-id="cjeacg" data-start="644" data-end="749">2X ready-to-use probe qPCR master mixes containing optimized buffers, dNTPs, and polymerase components.</li>
<li data-section-id="xfwfro" data-start="750" data-end="884">One-step probe RT-qPCR formats that combine reverse transcription and real-time PCR amplification in a single tube for RNA analysis.</li>
</ul>
<h2 dir="ltr"> How to Choose a Primer-Probe Master Mix</h2>
 Reference Dye Inclusion Primer-probe master mixes commonly include ROX as a passive reference dye for signal normalisation across wells on instruments that require it -- confirm whether your real-time PCR instrument needs ROX and whether the chosen master mix includes it or requires separate addition. Multiplexing Capability A core advantage of probe-based chemistry is multiplexing -- detecting multiple targets in a single well using probes labelled with spectrally distinct fluorophores. Confirm the master mix is validated for the number of multiplexed targets your assay requires. Probe Chemistry Compatibility While TaqMan-style hydrolysis probes are the most common, other probe chemistries such as Scorpion primers, exist; confirm the master mix is formulated for compatibility with your specific probe chemistry. Primer and Probe Concentration Guidelines Standard setups commonly use primer pairs at a final concentration around 0.5 &micro;M and probes at 0.25-0.5 &micro;M -- confirm the master mix manufacturer's recommended ranges, since probe concentration affects both signal intensity and background. One-Step vs. Two-Step Application Primer-probe master mixes are used in both two-step workflows (with a separately generated cDNA template) and as the chemistry component within one-step RT-qPCR kits -- confirm which configuration matches your workflow when selecting a product. 
<h2 dir="ltr"> Specifications Context</h2>
 In probe-based qPCR, the 5'-3' exonuclease activity of the Taq DNA polymerase cleaves the dual-labelled probe annealed to the target sequence during amplification, separating a fluorescent reporter from its quencher and generating signal proportional to target abundance -- the mechanism underlying the widely used TaqMan probe system. Because both primer and probe sequence must match the target for signal generation, probe-based assays generally offer higher specificity than dye-based detection, and because probes can be labelled with spectrally distinct fluorophores, multiple targets can be detected in a single well. Standard reaction setup adds primer pairs at approximately 0.5 &micro;M and probes at 0.25-0.5 &micro;M to the 2x master mix. Primer-probe master mixes are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light; lot-specific CoAs are available on request. 
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get optimized Primer-Probe real-time master mixes for your lab.

AzuraQuant Probe Fast qPCR Mix HiRox

AZ-2701 by Azura Genomics — AzuraQuant Probe Fast qPCR Mix HiRox; 2× probe-based mix; high ROX passive reference; TaqMan®, Scorpions®, molecular beacon compatible; antibody hot-start; standard and fast cycling; 100–5000 rxn formats.

AzuraQuant Probe Fast qPCR Mix HiRox 100 reactions

AzuraQuant Probe Fast qPCR Mix HiRox 500 Reactions

AzuraQuant Probe Fast qPCR Mix HiRox, 2,000 reactions

AzuraQuant Probe Fast qPCR Mix HiRox, 5,000 reactions

AzuraQuant Probe Fast qPCR Mix LoRox

AzuraQuant Probe Fast qPCR Mix LoRox is available in the following variants: AZ-2801, AZ-2805, AZ-2820, AZ-2850.AZ-2801 by Azura Genomics: AzuraQuant™ Probe Fast qPCR Mix LoRox — 2× master mix for TaqMan, Scorpions, and molecular beacon probe assays on standard and fast cycling instruments. Low ROX passive reference. Antibody hot-start. 100–10,000 rxn packs.

AzuraQuant Probe Fast qPCR Mix LoRox 100 reactions

AzuraQuant Probe Fast qPCR Mix LoRox 500 reactions

AzuraQuant Probe Fast qPCR Mix LoRox 2,000 reactions

AzuraQuant Probe Fast qPCR Mix LoRox 5,000 reactions

AzuraQuant Probe Fast qPCR Mix NoRox

Azura Genomics AzuraQuant™ Probe Fast qPCR Mix NoRox: 2× ready-to-use, Azura HS Taq, TaqMan/Scorpions/beacon compatible, no ROX, fast cycling. AZ-2901 (100), AZ-2905 (500), AZ-2920 (2,000), AZ-2950 (5,000 rxns).

AzuraQuant Probe Fast qPCR Mix NoRox 100 reactions

AzuraQuant Probe Fast qPCR Mix NoRox 500 Reactions

AzuraQuant Probe Fast qPCR Mix NoRox 2000 reactions

AzuraQuant Probe Fast qPCR Mix NoRox 5000 reactions

AzuraQuant cDNA Synthesis Kit - Product Sheet

AzuraQuant™ cDNA Synthesis Kit

The AzuraQuant™ cDNA Synthesis Kit for 2-step RT-qPCR and RT-PCR. Two-tube format: 5x Buffer and 20x HighTherm™ RT with RNase inhibitor by Azura Genomics is plus random hexamer and anchored oligo(dT) primers. RNA input from 4 pg. Available in 25, 100, and 500 reaction sizes. Available in 25 reactions (AZ-1995), 500 reactions (AZ-1995-50), and 100 reactions (AZ-1996).

AzuraQuant cDNA Synthesis Kit 25 Reactions

AzuraQuant cDNA Synthesis Kit 500 Reactions

AzuraQuant cDNA Synthesis Kit 100 reactions

SYBR/Dye-Based

<h2 dir="ltr">What is a SYBR Green Dye-Based Master Mix?</h2>
 A SYBR Green dye-based master mix is a 2x, ready-to-use formulation designed for dye-based, quantitative amplification of DNA targets, including cDNA and genomic DNA, by real-time PCR. SYBR Green dye binds to double-stranded DNA formed during amplification, and the resulting fluorescence increases proportionally to the quantity of double-stranded product, providing a real-time readout of amplification progress. Choose a SYBR Green dye-based master mix for cost-effective, flexible quantification across any primer pair and target combination, especially when running many different assays without the overhead of probe design.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="ic0lb3" data-start="180" data-end="319">SYBR Green dye-based qPCR master mixes for real-time quantification of DNA and cDNA targets across a wide range of research applications.</li>
<li data-section-id="1yz7ajv" data-start="320" data-end="449">Fast qPCR formulations with HiROX and LoROX options to support compatibility with different real-time PCR instrument platforms.</li>
<li data-section-id="1fbhq1p" data-start="450" data-end="586">Blue tracking dye master mixes, including AzuraView&trade; GreenFast and Accuris qMAX Green formats, for improved reaction setup visibility.</li>
<li data-section-id="1787obc" data-start="587" data-end="726">One-step SYBR Green RT-qPCR mixes that combine reverse transcription and real-time PCR in a single tube for RNA quantification workflows.</li>
<li data-section-id="1g0jipr" data-start="727" data-end="852">Ready-to-use 2x master mixes formulated for reproducible qPCR setup while reducing pipetting steps and workflow complexity.</li>
</ul>
<h2 dir="ltr"> How to Choose a SYBR Green Dye-Based Master Mix</h2>
 Dynamic Range and Sensitivity Look for master mixes offering a wide linear dynamic range -- some formulations provide precise measurement across approximately six logs of dynamic range, enabling accurate detection across both low- and high-concentration samples in the same assay design. Primer Melting Temperature Flexibility SYBR Green master mixes are commonly formulated to provide flexibility for primers with melting temperatures (Tm) in the range of approximately 55-65 &deg;C, reducing the need for extensive primer-specific optimisation when bringing new assays online. Bench-Top Stability Dual hot-start mechanisms in some SYBR Green master mixes provide extended bench-top stability -- up to 72 hours at room temperature in the dark in some formulations -- with single melt curves confirmed at both initial and post-incubation time points, supporting automated and batched workflows. Carryover Contamination Prevention Formulations that include uracil-DNA glycosylase (UDG) and dUTP help ensure leftover DNA residue from previous reactions is not re-amplified in subsequent experiments, a useful feature for high-throughput repeat assays. Instrument and Workflow Compatibility Broad instrument compatibility - including standard and fast cycling modes -- and compatibility with a variety of reverse transcriptase enzymes (for RT-qPCR workflows using a separately generated cDNA) widen the range of platforms a single master mix can support. 
<h2 dir="ltr"> Specifications Context</h2>
 SYBR Green dye is a fluorescent double-stranded DNA-binding dye that tracks amplification progress in real-time PCR: as the reaction proceeds, the dye binds newly synthesised dsDNA at each extension step, and the resulting fluorescence increase is proportional to product quantity. Because SYBR Green is not PCR-inhibitory and works with any primer-template combination, it provides accurate, reproducible results with high sensitivity across a broad dynamic range and gives consistent results across different real-time PCR platforms - one reason dye-based chemistry remains cost-effective compared to probe-based systems, which require a different labelled probe for each target sequence. SYBR Green master mixes are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light; lot-specific CoAs are available on request. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get high-yield SYBR/Dye-Based real-time PCR reagents for your lab.

AzuraQuant Green 1-Step qPCR Mix - Product Sheet

AzuraQuant™ Green 1-Step qPCR Mix HiRox

The AzuraQuant™ Green 1-Step qPCR Mix HiRox by Azura Genomics is AzuraQuant™ Green 1-Step qPCR Mix HiRox, 2× master mix + 20× AzuraSprint RT for one-step RT-qPCR directly from RNA. SYBR Green channel, HiRox for Applied Biosystems instruments. 20 µL reactions, −20°C storage, 18-month shelf life. Available 100–10,000 reaction packs. Available in 100 reactions (AZ-3501), 500 reactions (AZ-3505), 1,000 reactions (AZ-3510), and 5,000 reactions (AZ-3550).

AzuraQuant Green 1-Step qRT-PCR Kit Hi-Rox 100 Reactions

AzuraQuant Green 1-Step qPCR Mix HiRox 500 reactions

AzuraQuant Green 1-Step qRT-PCR Kit HiRox 1,000 reactions

AzuraQuant Green 1-Step qRT-PCR Kit HiRox 5,000 reactions

AzuraQuant™ Green 1-Step qPCR Mix LoRox

The AzuraQuant™ Green 1-Step qPCR Mix LoRox by Azura Genomics is a 2× one-step RT-qPCR master mix (low ROX) with a companion 20× thermostable AzuraSprint reverse transcriptase and non-inhibitory intercalating dye, enabling single-tube RNA quantification with post-amplification melt curve analysis on low-ROX compatible instruments. Available in 100 (AZ-3701), 500 (AZ-3705), 1,000 (AZ-3710), and 5,000 reactions (AZ-3750).