ABM Reagents

<h2 dir="ltr">What Are Adenoviral Control &amp; Reporter Constructs?</h2>
 Adenoviral over-expression experiments typically include matched control constructs that use the same vector backbone, promoter configuration, and production workflow as the experimental construct, and are packaged and purified under equivalent conditions. This helps account for vector-related and adenovirus-associated innate immune responses that can influence cellular readouts independently of the transgene. Using appropriate null or reporter controls is essential for accurate interpretation of adenoviral transduction and expression data. Choose the tools that support accurate data interpretation.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
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GFP Adenovirus Reporters: confirm transduction visually using high-titer viral particles expressing green fluorescent protein
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Beta-Galactosidase (LacZ) Adenoviruses: &beta;-gal markers derived from E. coli for standardized colorimetric and histochemical assays.
</li>
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pShuttle-LacZ Control Vectors: produce recombinant adenoviruses expressing LacZ.
</li>
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Null Adenovirus Controls: empty viral particles for assessment of non-specific cellular responses during gene delivery.
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CMV Null Adenoviruses: used to identify promoter-specific metabolic effects. Contains CMV promoter without a transgene.
</li>
</ul>
<h2 dir="ltr"> How to Choose Adenoviral Control &amp; Reporter Constructs</h2>
 Empty Vector Matching An empty vector control &mdash; identical backbone, promoter, and packaging as the experimental adenoviral construct, with no transgene insert &mdash; establishes the baseline for adenoviral delivery effects independent of the over-expressed gene. Fluorescent Reporter Selection GFP and RFP fluorescent reporters allow tracking of transduction efficiency by microscopy or flow cytometry, matching the promoter configuration of experimental constructs. Luciferase Reporter Format Firefly luciferase and Renilla luciferase provide quantitative bioluminescent readouts; secreted NanoLuc reporters allow sampling of culture supernatant for repeated, non-destructive measurements over a time course. Immune Activation Considerations Because adenoviral delivery activates innate immune sensing in transduced cells independently of any transgene, an identical-backbone control is essential to distinguish transgene-specific effects from generalised adenoviral response. Production Workflow Matching Controls should be produced, packaged, and purified using the same protocol and timeline as experimental constructs to avoid batch-to-batch variability confounding comparisons. 
<h2 dir="ltr"> Specifications Context</h2>
 Adenoviral delivery can activate innate immune sensing in transduced cells, altering gene expression independently of the transgene; therefore, matched controls with the same Ad5 backbone and promoter configuration are required for valid interpretation of adenoviral over-expression experiments. MBP adenoviral control and reporter constructs include GFP adenovirus, &beta;-gal (LacZ) adenovirus, and null adenovirus systems, supporting qualitative and quantitative assessment of transduction efficiency. Using control and experimental constructs produced under consistent conditions reduces variability that can confound downstream comparisons across treatment groups. Recording vector identity and batch information supports reproducibility and troubleshooting. Product availability reflects MBP&rsquo;s catalogue as of mid-2026. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today for the selection of matched adenoviral control and reporter constructs for your workflow.

Control/Reporter

Lentiviral Vector Amplification Protocol

AAV Infection Guideline

AAV Packaging Protocol

Selection-Drug Killing Curve

Lentivector Packaging Protocol

Lentivirus Infection Protocol

Adenovirus-2-Expression-Protocol

pShuttle-LacZ control vector

pShuttle-LacZ Control Vector (A004) by Applied Biological Materials is an adenoviral shuttle plasmid expressing CMV-driven E. coli β-galactosidase (LacZ), for use as a positive expression control in AdEasy-compatible adenoviral vector construction workflows. Detect by X-gal staining or ONPG assay.

Unit Quantity

Note

Shipping Condition

<h2 dir="ltr">What Are Adenoviral Cloning Vectors?</h2>
 An adenoviral shuttle plasmid (transfer vector) contains a transgene expression cassette flanked by adenoviral genomic sequences that mediate recombination into the full adenoviral backbone genome. This shuttle plasmid is the starting point for constructing any custom recombinant adenovirus. Successful adenoviral studies begin with the appropriate cloning backbone. Choose a vector compatible with your development workflow.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
pShuttle backbone vectors, including pShuttle(+), pShuttle(-), and pShuttle-His variants for gene insertion and adenoviral construct assembly&nbsp;
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Tagged expression vectors such as pShuttle-His-18x and pShuttle-2A-RFP for co-expression and protein labeling studies
</li>
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Functional assay and reporter vectors, including pPromoter and pSiShuttle for transcriptional analysis and gene regulation&nbsp;
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Ready-to-use adenoviral DNA for simplified preparation of constructs
</li>
</ul>
<h2 dir="ltr"> How to Choose an Adenoviral Cloning Vector</h2>
 Promoter Selection CMV, EF1&alpha;, and CAG promoter options are available for driving transgene expression &mdash; CMV offers strong, broadly active expression, while EF1&alpha; and CAG can provide more consistent expression in certain cell types. Cloning Method Compatibility Gateway-compatible backbones enable site-specific recombination cloning without restriction enzymes, useful for parallel construct generation. Standard multiple cloning sites (MCS) with conventional restriction sites remain available for traditional cloning workflows. Deletion Configuration E1/E3-deleted Ad5 backbones are the standard configuration &mdash; E1 deletion renders the virus replication-deficient, while E3 deletion provides additional cloning capacity. Downstream Production Pathway The shuttle plasmid recombines into the full adenoviral genome during virus production &mdash; selecting a shuttle vector compatible with your packaging cell line and downstream amplification approach avoids compatibility issues later in the workflow. Reporter/Selection Inclusion Shuttle vectors with fluorescent reporters (GFP) or selection markers built into the cassette simplify confirmation of successful cloning and subsequent virus packaging steps. 
<h2 dir="ltr"> Specifications Context</h2>
 After cloning the transgene into an adenoviral shuttle plasmid, the construct is recombined into the full adenoviral backbone genome in packaging systems or recombination-based workflows, followed by amplification in complementing cell lines to generate recombinant adenoviral stocks. Gateway-compatible shuttle vectors enable transfer of validated entry clones into the adenoviral system without re-cloning. Sequence verification of the shuttle construct by restriction analysis or sequencing is recommended before recombination to ensure construct integrity. Product availability reflects MBP&rsquo;s catalogue as of mid-2026. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP for guidance on shuttle vector selection based on promoter configuration and experimental design requirements.

Cloning Vectors

pSiShuttle Vector

A012 by Applied Biological Materials is the pSiShuttle plasmid (5.0 µg) of the Adeno-4™ Adenovirus Expression System, featuring multiple unique MCS cloning sites for shRNA/siRNA cassette insertion. Use to construct recombinant adenoviral RNAi vectors for transient, non-integrating gene knockdown in primary cells, non-dividing cells, and in vivo models. Store at &minus;20°C.

Vector

<h2 data-section-id="1go10h8" data-start="173" data-end="215">What are classic cell culture media?</h2>
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Classic cell culture media refers to the foundational basal medium formulations that have underpinned mammalian cell culture since the mid-20th century: DMEM (Dulbecco's Modified Eagle Medium), MEM (Minimum Essential Medium), RPMI 1640, Ham's Nutrient Mixtures F-10 and F-12, IMDM (Iscove's Modified Dulbecco's Medium), and the combination formulation DMEM/F-12. Each is manufactured according to its originally published formula, with modifications limited to those necessary for consistent performance and stability of the medium over its shelf life. These media supply the amino acids, vitamins, glucose, salts, and (in most formulations) phenol red pH indicator that form the nutritional backbone of essentially all subsequent specialty, serum-free, and Advanced media derivatives.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Selective Mammalian Antibiotics including Puromycin, Geneticin, and Hygromycin B for stable development of cell lines.</li>
<li dir="ltr" role="presentation">Broad-Spectrum Bactericidal Agents for routine contamination prevention, including Penicillin/Streptomycin and Gentamicin&nbsp;</li>
<li dir="ltr" role="presentation">Prokaryotic Selection Reagents for effective bacterial transformation, including Ampicillin, Kanamycin, Chloramphenicol, and Tetracycline&nbsp;</li>
<li dir="ltr" role="presentation">Antifungal and Antimycotic Solutions to maintain sterile cultures, including combined 100X cocktails and Amphotericin B (Fungizone)&nbsp;</li>
<li dir="ltr" role="presentation">Specialized Small Molecules for targeted biochemical assays, including Ganciclovir, Rapamycin, and Actinomycin D&nbsp;</li>
</ul>
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<h2 data-section-id="1mfajxk" data-start="1218" data-end="1252">How to choose a classic medium</h2>
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DMEM vs MEM
DMEM contains up to four times the amino acid and vitamin concentration of original Eagle's MEM, along with higher glucose levels, making it suitable for cell types with elevated nutrient demands &mdash; DMEM is broadly applicable across mammalian, insect, and plant cell phenotypes including primary cells, stem cells, and transformed lines. MEM remains widely used for monolayer culture of many standard cell types, with optional Hanks' or Earle's salt formulations and a calcium-free modification that permits suspension growth.
RPMI 1640 for suspension and hematopoietic culture
RPMI 1640, developed at the Roswell Park Memorial Institute for culturing human leukemic cells, is optimized for non-adherent cell types &mdash; lymphocytes and other blood cells &mdash; and contains glutathione plus higher biotin, B12, and PABA concentrations than DMEM or MEM. Despite its hematopoietic origin, RPMI 1640 supports a broad range of adherent lines (HeLa, T47D, RENCA, CT26.WT) when properly supplemented.
Ham's F-10/F-12 for CHO and specialized applications
Ham's nutrient mixtures were originally developed for CHO cell clones, HeLa cells, and mouse L-cells, and remain suited to hepatocyte culture, viral fusion experiments, and toxicity assays, with formulations available for use with or without serum. Coon's modification of Ham's F-12 roughly doubles the amino acid and pyruvate content and adds ascorbic acid, developed specifically for hybrid cells produced by viral fusion.
DMEM/F-12 for complex, rich-medium applications
DMEM/F-12 (1:1 mixture) combines DMEM's glucose, amino acid, and vitamin richness with Ham's F-12's zinc, putrescine, hypoxanthine, and thymidine, producing an extremely rich complex medium used for MDCK cells, glial cells, fibroblasts, and human endothelial cells &mdash; and serving as the basal medium for many serum-free Advanced formulations used in organoid and stem cell work.
IMDM for high-density rapid proliferation
IMDM further enriches DMEM with additional amino acids and vitamins, substituting potassium nitrate for ferric nitrate and adding HEPES and sodium pyruvate. IMDM's sodium bicarbonate buffer system (5&ndash;10% CO₂) and enriched composition make it well suited for rapidly proliferating, high-density cultures including Jurkat, COS-7, and macrophage cells.
Liquid vs. powder format
Liquid classic media are ready to use (after appropriate FBS and supplement addition) and include sodium bicarbonate in the formulation. Powdered media are manufactured without sodium bicarbonate, since it tends to off-gas in powder form &mdash; 3.7 g/L sodium bicarbonate must be added upon reconstitution, followed by sterile filtration. Powder formats offer cost and shipping-weight advantages for high-volume labs preparing their own media.
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<h2 data-section-id="17i0pj" data-start="5035" data-end="5061">Specifications context</h2>
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Classic media are manufactured according to originally published formulas, with stability-related modifications documented per product. DMEM's sodium bicarbonate buffer system at 3.7 g/L is standard across liquid formulations; powdered formats require this addition upon reconstitution along with 0.22 &micro;m sterile filtration. As of 2026, DMEM, DMEM/F-12, and RPMI 1640 remain the three most widely specified classic media across academic and biomanufacturing labs, with IMDM increasingly used for high-density suspension applications in bioprocessing.
For recombinant growth factors and cytokines used alongside classic media, see Recombinant Proteins. For supplementation reagents (GlutaMAX, NEAA, sodium pyruvate, HEPES), see Cell Culture Supplements &amp; Additives. Browse Mammalian Cell Culture for the broader category.
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP to get highly efficient microbial control solutions for your lab.

Antibiotics

MSDS

Puromycin

Puromycin G264 — 10 mg/ml ready-to-use solution (1.0 ml) from Streptomyces alboniger. Aminonucleoside antibiotic for stable mammalian cell selection via pac gene resistance. Working concentration 1–10 µg/ml. Store at 4 °C (stable ≥1 year). RUO.

Concentration

Recommended Protocol

Storage Condition

Shipping condition

<h2 dir="ltr">What Are Specialized Cloning Vectors &amp; Viral Tools?</h2>
 Standard lentiviral and retroviral expression vectors meet the needs of most overexpression and knockdown workflows, but many cell biology and translational research questions demand purpose-engineered constructs with specialised functions &mdash; subcellular localisation, multi-gene co-expression, or conditional activation. Select the format best suited to your experimental requirements.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/dna/cloning-services-products/specialized-cloning-vectors/-virus/organelle-labeling-viruses">Organelle Labeling Viruses: </a>for live-cell visualization and functional pathway analysis of cellular structures in mammalian systems
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Targeted fluorescent labeling of specific cellular structures using viral delivery.
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Stable expression of organelle-specific markers for long-term live-cell imaging.
</li>
<li dir="ltr" aria-level="1">
Compatible with fluorescence microscopy for dynamic cellular imaging applications.
</li>
<li data-section-id="8k7pm2" data-start="506" data-end="603" data-is-last-node="">Suitable for studying organelle organization, transport, and cellular function in living cells.</li>
</ul>
<h2 dir="ltr"> How to Choose Specialized Cloning Vectors</h2>
 Subcellular Targeting Needs Organelle-targeted fluorescent labeling lentiviral particles deliver fluorescent protein constructs targeting mitochondria, endoplasmic reticulum, nucleus, Golgi, lysosome, peroxisome, plasma membrane, autophagosome, and cytoskeleton &mdash; providing a stable alternative to chemical dyes for live-cell imaging. Multi-Gene Co-Expression Bicistronic vectors using IRES or 2A self-cleaving peptide sequences enable co-expression of two genes (e.g., a gene of interest and a fluorescent marker) from a single transcript or vector. Conditional Expression Requirements Conditional DIO/FLEX vectors restrict transgene expression to Cre-expressing cells, essential for circuit-specific neuroscience research and in vivo conditional gene expression studies. Temporal Control Needs Inducible constructs (Tet-On/Tet-Off systems) provide doxycycline-regulated transgene activation for experiments requiring temporal control over expression timing. Delivery Format Most specialised constructs are available as lentiviral particles for stable, long-term expression in both dividing and non-dividing cell types, including primary cells and in vivo models. 
<h2 dir="ltr"> Specifications Context</h2>
&nbsp;
Traditional subcellular organelle imaging relies on chemical dyes (e.g., MitoTracker, LysoTracker, ER-Tracker) or antibody-based immunofluorescence in fixed cells; however, chemical dyes are transient and may exhibit cytotoxicity at working concentrations, while antibody-based methods are limited to endpoint imaging. Viral organelle-targeted fluorescent expression systems enable stable, genetically encoded labeling for long-term live-cell imaging and dynamic subcellular tracking. These constructs are supplied in plasmid DNA and pre-packaged viral formats and support applications including organelle dynamics, trafficking studies, and multicolor imaging workflows. Specialized cloning vectors and viral tools are sourced from established manufacturers, including Applied Biological Materials (ABM), and product availability reflects MBP&rsquo;s catalogue as of mid-2026.
&nbsp;
<a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact the MBP team </a>today and get specialized cloning vectors for your lab.

Specialized Cloning Vectors/ Viruses

Specialized Cloning Vectors/ Virus

Pwpxl-mCh-Opto-CRAC vector

AX0010025 Pwpxl-mCh-Opto-CRAC Vector by Applied Biological Materials is a pWPXL lentiviral plasmid encoding the mCherry-fused Opto-CRAC component for light-activated CRAC channel calcium entry. Use together with AX0010026 (Pwpxl-ORAI1-(Opto-CRAC)2) to reconstitute the complete two-component optogenetic CRAC system. Store at &minus;20&deg;C or below.

Pwpxl-NFAT-mCh

AX0010027 by Applied Biological Materials is a pWPXL lentiviral plasmid (1.0 µg) expressing mCherry under NFAT response elements. Provides a red-fluorescent, quantitative readout of NFAT transcriptional activity for T cell activation studies, NFAT pathway reporter assays, drug screening, and live-cell imaging of NFAT dynamics. mCherry excitation ~587 nm, emission ~610 nm.

Pwpxl-ORAI1-(Opto-CRAC)2 vector

AX0010026 Pwpxl-ORAI1-(Opto-CRAC)2 Vector by Applied Biological Materials is a pWPXL lentiviral plasmid encoding ORAI1 fused to two Opto-CRAC light-sensing modules for optogenetic CRAC-mediated calcium entry. Must be co-expressed with AX0010025 (mCh-Opto-CRAC) to reconstitute the complete Opto-CRAC system. Store at &minus;20&deg;C or below.

<h2 dir="ltr">What is a qPCR, Titer Kit?</h2>
 A qPCR titer kit provides the primers, probes, or dye-based detection chemistry, standards, and master mix components needed to quantify viral vector genome copy number by real-time PCR. For lentiviral preparations, this typically involves amplifying a conserved region of the viral genome (such as the LTR or a packaging signal sequence) and comparing the signal to a standard curve of known copy number to determine vector genomes per mL of the preparation. Use a qPCR titer kit for rapid, same-day genomic titer determination of in-house viral vector preparations, understanding that genomic titer (genome copies/mL) is related to, but distinct from, functional (infectious) titer measured by transduction assays.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Lentivirus &amp; Retrovirus Titer Kits: Advanced quantitative qPCR-based tools designed for rapid and accurate assessment of viral genome copy number in lentiviral and retroviral preparations, supporting consistent transduction workflow optimisation in mammalian cell culture systems.
</li>
<li dir="ltr" aria-level="1">
AAV Titer Kits: Optimised qPCR solutions for the quantification of Adeno-Associated Virus vectors, providing precise genome copy data required for vector dosing and therapeutic research studies.
</li>
</ul>
<h2 dir="ltr"> How to Choose a qPCR Titer Kit</h2>
 Viral Vector Type Compatibility qPCR titer kits are commonly available for lentivirus, with related kits for AAV and other viral vector types -- confirm the kit targets the appropriate conserved genomic region for your specific vector type and packaging system. Genomic vs. Functional Titer qPCR titer kits report genomic titer (vector genome copies per mL), which can differ from functional or infectious titer measured by transduction-based assays (such as a GFP or antibiotic-resistance endpoint assay) -- understanding this distinction is important when translating a qPCR titer result into an experimental MOI. Standard Curve and Quantification Range The kit's included standards define the quantification range over which results are reliable -- confirm your expected titer falls within this range, diluting samples as needed to bring concentrated preparations into range. Turnaround Time qPCR titer kits provide same-day results (the time required for a qPCR run plus data analysis), substantially faster than functional transduction-based titer assays, which require a transduction step followed by several days of cell culture and an endpoint readout. Compatibility with Existing qPCR Infrastructure Since qPCR titer kits use standard real-time PCR instrumentation, confirm that the kit's detection chemistry (dye-based or probe-based) and any reference dye requirements (such as ROX) are compatible with your existing real-time PCR instrument. 
<h2 dir="ltr"> Specifications Context</h2>
 qPCR-based viral titer determination amplifies a conserved genomic region of the vector -- for lentivirus, commonly within the LTR or packaging signal -- and compares the resulting signal against a standard curve of known copy number to calculate vector genomes per mL of the test preparation. Because genomic titer counts total vector particles containing the packaged genome regardless of whether they are capable of productive transduction, it typically yields a higher number than functional titer, which counts only particles that successfully transduce and express in target cells; researchers calculating MOI for an experiment should be clear about which titer type they are using, since the appropriate MOI range can differ depending on which titer figure is the basis for the calculation. qPCR titer kits are typically stored at &minus;20 &deg;C, with standards and master mix components included; lot-specific CoAs are available on request. 
Ensure the accuracy of your viral dosing&mdash;<a href="https://mbpinc.net/contact-us" style="color: #236fa1;">reach out to the MBP team</a> now for a quote on our expert qPCR Titer solutions

qPCR Titer Kits

DataSheet

ROX Machine Compatibility

qPCR AAV Titer Kit

G931 by Applied Biological Materials is a 100-reaction qPCR AAV titer kit. One-step lysis + qPCR with proprietary NTC-eliminating primers. Includes DNase I Reaction Mix for unencapsidated DNA removal. SYBR Green–comparable dye; ROX supplied separately. ISO 13485:2016 MDSAP certified. Store at −20°C; stable 2 years.

Flyer

qPCR Lentivirus Titer Kit

LV900 by Applied Biological Materials is a 100-reaction one-step RT-qPCR lentiviral titer kit. No RNA purification required — direct use on clarified supernatant. Proprietary primer design eliminates NTC amplification. SYBR Green–comparable dye; ROX supplied separately. ISO 13485:2016 MDSAP certified. Store at −20°C; stable 2 years.

<h2 dir="ltr"> What Are Lentiviral Purification &amp; Packaging Kits?</h2>
 Producing high-quality lentiviral stocks is a prerequisite for reproducible transduction experiments. Low-titer or impure virus preparations reduce transduction efficiency, introduce toxicity from residual cell debris, and compromise experimental reproducibility. Purification and packaging kits address every step from plasmid transfection through virus harvest and concentration. Vector production quality depends on efficient purification and packaging workflows. Choose the kit designed for your system.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
2nd and 3rd Generation Lentivirus Bundles combining transduction enhancers, transfection reagents, packaging mix, titer kits for complete workflows
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
2nd and 3rd Generation Packaging System Mix for standardized viral production workflows
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
DNAfectin&trade; Plus and Packaging Mix systems for enhanced transfection workflows
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
Lentivirus Promoter Blast&trade; Kit for lentiviral expression studies driven by promoters
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
SARS-CoV-2 S Protein Pseudotype Lentiviral Packaging Mix for pseudovirus-based research applications
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
Ultra-Pure Lentivirus Purification Kit for viral cleanup and concentration
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
ViralEntry&trade; Transduction Enhancer for improved transduction
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
qPCR Lentivirus Titer Kit &amp; ViralEntry&trade; Bundle for optimized transduction workflows and&nbsp; viral quantification&nbsp;
</li>
</ul>
<h2 dir="ltr"> How to Choose a Purification &amp; Packaging Kit</h2>
 Concentration Method Available PEG precipitation kits work with a standard benchtop centrifuge, producing 10&ndash;100-fold concentration &mdash; the most accessible method for labs without ultracentrifuges. Ultrafiltration (100 kDa cutoff) offers high recovery (&gt;80%) while removing small molecules and unincorporated VSV-G. Ultracentrifugation provides the highest concentration (up to 1000&times;) but requires specialised equipment. Packaging Plasmid Generation 3rd generation systems (pMDLg/pRRE + pRSV-Rev + pVSV-G) split packaging elements across three plasmids and eliminate Tat for biosafety. 2nd generation systems use a single packaging plasmid + pVSV-G; some transfer vectors with RSV promoters produce lower titers with 3rd generation packaging unless using CMV-promoter variants (pLKO.1C). Starting Volume Scale Ultrafiltration units are compatible with both small-scale (&le;20 mL) and large-scale (up to 1 L) starting volumes using appropriately sized membranes. Plasmid Transfection Ratio For 3rd generation packaging, a 4:2:1:1 ratio of transfer vector:pMDLg/pRRE:pVSV-G:pRSV-Rev has been validated to maximise titer, with 20 &micro;g transfer vector per 15 cm dish as a standard starting point. Titer Assessment Method p24 ELISA measures total viral particle content; functional transduction assays (GFP or antibiotic selection) measure infectious titer; qPCR-based RNA titration measures packaged RNA copies. Functional titer is most relevant for transduction experiments. 
<h2 dir="ltr"> Specifications Context</h2>
 Lentiviral concentration workflows commonly combine PEG precipitation, ultrafiltration, or density-gradient methods to increase viral titer without requiring ultracentrifugation. Ultrafiltration systems using 100 kDa membranes efficiently retain lentiviral particles (approximately 80&ndash;120 nm) while removing smaller contaminants and free proteins. Following concentration, viral stocks are typically aliquoted into single-use volumes and stored at &minus;80 &deg;C because infectivity decreases with repeated freeze&ndash;thaw cycles and prolonged storage above freezing temperatures. Modern 2nd- and 3rd-generation packaging systems separate essential viral functions across multiple plasmids, including a dedicated VSV-G envelope plasmid, to support safer lentiviral production workflows. Product availability reflects MBP's catalogue as of mid-2026. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP Inc. and get reliable lentiviral purification and packaging kits for your lab today.

Purfication & Packaging Kits

MSDS- LV900

MSDS - G515

qPCR Lentivirus Titer Kit & ViralEntry™ Bundle

LV900-G515 by Applied Biological Materials is a starter bundle combining the qPCR Lentivirus Titer Kit (LV900; one-step RNA extraction + RT-qPCR) and ViralEntry™ Transduction Enhancer 100X (G515; up to 10X transduction boost in diverse cell types including primary T cells). Shipped on Ice Pack. Ideal for lentiviral workflow setup from titer determination to enhanced transduction.

qPCR Retrovirus Titer Kit

G949 by Applied Biological Materials is the qPCR Retrovirus Titer Kit — 100 reactions. One-step RT-qPCR directly from viral supernatant; no purification needed. BlasTaq™ MasterMix (SYBR/EvaGreen-comparable dye); proprietary primers — no NTC amplification. ISO 13485:2016 MDSAP Certified. ROX Reference Dye included. 2-year stability. Store at −20°C.

<h2 dir="ltr"> What Are Miscellaneous PCR Items?</h2>
 Miscellaneous Items groups supporting reagents used alongside core PCR enzymes, master mixes, and nucleotide components, covering essential but non-core materials required for PCR and RT-PCR workflows. This includes nuclease-free water for reaction setup and dilution, Random Primer (6N) and Oligo(dT)18 primers for cDNA synthesis, and ionic solutions such as MgCl₂, MgSO₄, NaCl, and MnCl₂ used for reaction optimisation. The category also includes certified nuclease-free water options from suppliers such as BIOLOGIX for sensitive molecular biology applications. Use this category to source essential supporting reagents that complete PCR-based experimental workflows alongside primary amplification products. 
&nbsp;
<h2 data-start="432" data-end="459">What you will find:</h2>
&nbsp;
<ul data-start="461" data-end="1220">
<li data-section-id="b5abnd" data-start="461" data-end="635">Nuclease-Free Water: DNase/RNase-free water suitable for PCR, RT-PCR, qPCR, and nucleic acid preparation workflows, including certified molecular biology-grade options.</li>
<li data-section-id="n5mfl0" data-start="636" data-end="789">Primers &amp; Oligos: Random Primer (6N) and Oligo(dT)18 formats for reverse transcription, cDNA synthesis, and related molecular biology applications.</li>
<li data-section-id="1x29qzm" data-start="790" data-end="932">Reaction Components: Molecular biology-grade MgCl₂, MgSO₄, MnCl₂, and NaCl solutions for reaction preparation and protocol optimisation.</li>
<li data-section-id="16hzrat" data-start="933" data-end="1075">Workflow Support Reagents: Essential supporting materials used alongside PCR enzymes, master mixes, and nucleic acid amplification kits.</li>
<li data-section-id="4t6tsy" data-start="1076" data-end="1220">Research-Ready Formats: Convenient reagent sizes suitable for routine academic, biotechnology, and molecular biology laboratory workflows.</li>
</ul>
<h2 dir="ltr"> How to Choose Miscellaneous PCR Items</h2>
 Gel Loading Dyes Loading dyes add density and colour to PCR products for gel electrophoresis, allowing visual tracking of sample migration. Some 2x master mixes include loading dye pre-formulated, but a separate loading dye is needed when using dye-free master mixes or individual-component PCR setups. PCR Enhancers and Additives Additives such as betaine, DMSO, or proprietary enhancer formulations can improve amplification of GC-rich or otherwise difficult templates by reducing secondary structure or improving primer annealing specificity -- useful as a troubleshooting tool when a standard reaction underperforms on a specific target. Nuclease-Free Water Grade Nuclease-free water is used for reaction dilutions, resuspending primers and probes, and as a no-template control in PCR and qPCR setups. Confirm the water is certified free of detectable RNase and DNase activity, particularly for RNA-handling workflows. PCR Tube and Plate Format PCR tubes, strips, and plates are available in formats matching common thermal cycler block configurations -- 0.2 mL individual tubes, 8-well or 12-well strips, and 96-well plates -- select the format matching your thermal cycler and throughput needs. Compatibility with Primary Reagents When ordering supporting consumables alongside primary PCR reagents, confirming tube/plate compatibility with both your thermal cycler and any downstream automation (liquid handlers, plate readers) avoids workflow bottlenecks from mismatched formats. 
<h2 dir="ltr"> Specifications Context</h2>
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Nuclease-free water used in molecular biology workflows is typically certified through DNase/RNase activity testing and supplied in formats suitable for routine PCR, RT-PCR, and nucleic acid dilution applications. Random Primer (6N) and Oligo(dT)18 primers are used in reverse transcription workflows to support cDNA synthesis from RNA templates. Ionic solutions such as MgCl₂, MgSO₄, NaCl, and MnCl₂ are used to support and optimise polymerase activity under different reaction conditions. Most items in this category are stable under standard laboratory storage conditions, with product-specific handling and storage requirements provided by the manufacturer.
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Miscellaneous Items

Miscellaneous items

Random Primer (6N)

Random Primer (6N) G139 — 10 µM random hexamers in ddH₂O, 500 µl. For reverse transcription cDNA synthesis from total RNA or mRNA. Recommended final concentration: 0.5–1 µM. Store at −20 °C up to 60 months; avoid freeze–thaw cycles.

Ready-to-Use Adeno DNA (Pre-linearized by I-Ceul/PI-SceI)

A001 by Applied Biological Materials is Ready-to-Use Adeno DNA pre-linearized with I-CeuI/PI-SceI — no user restriction digest required. Direct ligation with compatible shuttle plasmid inserts for rapid recombinant E1-deleted adenoviral vector construction. Compatible with A003, A008, A020 shuttle vectors. Store at −20°C or below.