Name: pSiShuttle Vector
SKU: A012
Price: 325.50 USD
Availability: InStock

<h2 dir="ltr">What Are Adenoviral Cloning Vectors?</h2>
 An adenoviral shuttle plasmid (transfer vector) contains a transgene expression cassette flanked by adenoviral genomic sequences that mediate recombination into the full adenoviral backbone genome. This shuttle plasmid is the starting point for constructing any custom recombinant adenovirus. Successful adenoviral studies begin with the appropriate cloning backbone. Choose a vector compatible with your development workflow.
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<h2 dir="ltr">What you will find:</h2>
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pShuttle backbone vectors, including pShuttle(+), pShuttle(-), and pShuttle-His variants for gene insertion and adenoviral construct assembly&nbsp;
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Tagged expression vectors such as pShuttle-His-18x and pShuttle-2A-RFP for co-expression and protein labeling studies
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Functional assay and reporter vectors, including pPromoter and pSiShuttle for transcriptional analysis and gene regulation&nbsp;
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Ready-to-use adenoviral DNA for simplified preparation of constructs
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<h2 dir="ltr"> How to Choose an Adenoviral Cloning Vector</h2>
 Promoter Selection CMV, EF1&alpha;, and CAG promoter options are available for driving transgene expression &mdash; CMV offers strong, broadly active expression, while EF1&alpha; and CAG can provide more consistent expression in certain cell types. Cloning Method Compatibility Gateway-compatible backbones enable site-specific recombination cloning without restriction enzymes, useful for parallel construct generation. Standard multiple cloning sites (MCS) with conventional restriction sites remain available for traditional cloning workflows. Deletion Configuration E1/E3-deleted Ad5 backbones are the standard configuration &mdash; E1 deletion renders the virus replication-deficient, while E3 deletion provides additional cloning capacity. Downstream Production Pathway The shuttle plasmid recombines into the full adenoviral genome during virus production &mdash; selecting a shuttle vector compatible with your packaging cell line and downstream amplification approach avoids compatibility issues later in the workflow. Reporter/Selection Inclusion Shuttle vectors with fluorescent reporters (GFP) or selection markers built into the cassette simplify confirmation of successful cloning and subsequent virus packaging steps. 
<h2 dir="ltr"> Specifications Context</h2>
 After cloning the transgene into an adenoviral shuttle plasmid, the construct is recombined into the full adenoviral backbone genome in packaging systems or recombination-based workflows, followed by amplification in complementing cell lines to generate recombinant adenoviral stocks. Gateway-compatible shuttle vectors enable transfer of validated entry clones into the adenoviral system without re-cloning. Sequence verification of the shuttle construct by restriction analysis or sequencing is recommended before recombination to ensure construct integrity. Product availability reflects MBP&rsquo;s catalogue as of mid-2026. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP for guidance on shuttle vector selection based on promoter configuration and experimental design requirements.

Cloning Vectors

Selection-Drug Killing Curve

Adenovirus-2-Expression-Protocol

pSiShuttle Vector

A012 by Applied Biological Materials is the pSiShuttle plasmid (5.0 µg) of the Adeno-4™ Adenovirus Expression System, featuring multiple unique MCS cloning sites for shRNA/siRNA cassette insertion. Use to construct recombinant adenoviral RNAi vectors for transient, non-integrating gene knockdown in primary cells, non-dividing cells, and in vivo models. Store at &minus;20°C.