ABM Reagents

<h2>What is PriGrow Media?</h2>
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PriGrow Media is a proprietary series of basal cell culture media developed and distributed by MBP in partnership with ABM (Applied Biological Materials), where each individual PriGrow formulation, such as PriGrow I, PriGrow IV, or PriGrow X, has a distinct composition of HEPES and bicarbonate buffers, nutrient concentrations, and basal supplements specifically optimized to support the growth of a defined set of specialized cell lines or primary cell types. Unlike standard media like DMEM or RPMI that are broadly formulated for a wide range of cell lines, each PriGrow formulation is a targeted solution for cell types that do not grow well, or grow inconsistently, in standard mammalian cell culture media.
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<h2 dir="ltr">What you will find:</h2>
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Endothelial and epithelial formulations optimized to preserve critical barrier functions and&nbsp;unique physiological phenotypes in specialized vascular cell lines.
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Myogenic media for the smooth and skeletal muscle cells in vitro proliferation, functional maintenance, and structural preservation
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Hepatic induction systems for supporting liver cell research, drug metabolism profiling, and long-term functional assays.
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Serum-free, chemically defined solutions for regulation of multi-lineage differentiation, progenitor line expansion, and high-yield recovery after thawing.
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<h2>How to use PriGrow Media</h2>
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Identify the correct PriGrow variant for your cell type
Each MBP primary or specialized cell line product lists the recommended PriGrow variant in its data sheet. Using the wrong variant may result in suboptimal growth or inconsistent results, so matching the variant to the cell type is the critical first step.
Add FBS at 10% to make the basal PriGrow medium into complete media
PriGrow media are supplied as serum-free basal media and must be supplemented with 10% fetal bovine serum to provide the growth factors, carrier proteins, and lipid supplements that cells require for robust growth. Simply adding FBS at 10% of the final volume to the PriGrow basal medium produces complete media ready for use.
Optionally add antibiotics to complete PriGrow media
Penicillin-streptomycin with or without amphotericin B can be added to complete PriGrow media to reduce contamination risk, though antibiotic-free culture is standard in many labs to avoid masking low-level contamination and maintain cell health in long-term culture.
Warm PriGrow media to 37&deg;C before adding to cells
Like all cell culture media, PriGrow basal media and complete PriGrow media should be pre-warmed in a water bath at 37&deg;C before use to avoid cold-shock stress on cells that could affect growth and viability during media changes or passaging.
Maintain cultures at 37&deg;C under 5% CO₂
PriGrow media are formulated for standard mammalian cell culture conditions of 37&deg;C and 5% CO₂, which maintains physiological pH in the bicarbonate-buffered medium, unless the specific cell type protocol calls for different conditions.
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<h2>Specifications context</h2>
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PriGrow media contain phenol red as a standard pH indicator and are provided as serum-free basal media, with each formulation's HEPES and bicarbonate buffer ratios, nutrient levels, and supplemental additives matched to the cell types it is optimized for. As of 2026, specialized primary cell media like PriGrow remain a distinct product category from standard broad-spectrum media, since primary cells and cell-type-specific in vitro models require media compositions that better match in vivo nutritional conditions than DMEM or RPMI provides.
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get high-quality PriGrow media for your lab.

PriGrow Media

Prigrow XV Medium

TM027 by Applied Biological Materials is Prigrow XV Medium (500 mL), a specialized antibiotic-free cell culture medium formulated for THP-1 human monocytic cells, with lot-tested pH 7.0–7.6, osmolarity 264–292 mOsm/kg, and endotoxin ≤1 EU/mL — used for macrophage differentiation, NLRP3 inflammasome research, and innate immunity assays.

Unit Quantity

Endotoxin Level

Osmolarity

Sterility

Appearance

Storage Condition

Caution

Disclaimer

Shipping Condition

Prigrow XVIII Medium

TM039 by Applied Biological Materials (ABM) is Prigrow XVIII Medium, a 500 mL liquid cell culture medium for primary mammalian cell culture. Each lot is tested for sterility, pH, osmolality, endotoxin, adherence rate, morphology, and cellular metabolism before release.

<h2 data-section-id="5jwp5g" data-start="0" data-end="46">What are ligation independent cloning kits?</h2>
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Ligation independent cloning (LIC) kits enable assembly of PCR-amplified DNA fragments into a linearized vector through complementary single-stranded overhangs, generated either by exonuclease treatment of vector and insert ends or by PCR primers carrying homology arms, bypassing the need for restriction enzyme digestion and T4 DNA ligase. Related entities include seamless cloning methods (Gibson Assembly-type approaches), directional cloning, multi-fragment assembly, homology arm design, and downstream transformation into competent cells.
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<h2 dir="ltr">What you will find:</h2>
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<a href="https://mbpinc.net/product/pro-ligation-free-cloning-kit">Pro Ligation-Free Cloning Kit:</a> Ligase-independent, comprehensive, all-in-one system for smooth and rapid DNA assembly.
</li>
</ul>
&nbsp;
<h2 data-section-id="5tbqjl" data-start="792" data-end="842">How to choose ligation independent cloning kits</h2>
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Single-fragment vs. multi-fragment assembly
Some LIC kits are optimized for inserting a single PCR fragment into a vector at a defined site, while others support simultaneous assembly of multiple fragments (often up to 4&ndash;5) into a single linearized vector in one reaction; choose based on the complexity of the construct being built.
Homology arm length and primer design
LIC and seamless assembly methods require PCR primers with homology arms (typically 15&ndash;25 base pairs) overlapping the vector or adjacent fragment ends; primer design tools provided with some kits can help ensure compatible overlap lengths and melting temperatures across all fragment junctions.
Compatibility with existing vector backbones
Confirm the LIC kit's enzyme system is compatible with the vector backbone already in use, particularly for specialized vectors such as lentiviral transfer plasmids, since some seamless assembly chemistries have sequence requirements at the junction sites.
Kit completeness (enzyme-only vs. full kit with competent cells)
Standalone enzyme/buffer kits assume the lab already has compatible competent cells for transformation, while full kits bundle competent cells (and sometimes a control vector/insert pair) for initial validation of the assembly chemistry before applying it to a novel construct.
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<h2 data-section-id="1fljvas" data-start="3053" data-end="3078">Specifications context</h2>
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LIC and seamless assembly kits report cloning efficiency as the percentage of correct-assembly colonies among total transformants, with well-optimized reactions commonly exceeding 80&ndash;90% correct assembly for 2&ndash;3 fragment reactions. Reaction setup typically takes 15 minutes to 1 hour at a defined temperature (often room temperature or 50&deg;C depending on the chemistry) before transformation.&nbsp;
This sub-category is part of MBP's cloning catalog alongside transformation kits and competent cells, needed to propagate the assembled construct, and chemicals/reagents for general cloning consumables. Labs building custom immortalization vectors can also review MBP's cell immortalization catalog. MBP's specialist team can help confirm assembly chemistry compatibility with a specific vector backbone before order placement.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today to get high-integrity ligation-independent cloning kits for your lab.

Ligation Independent Cloning Kits

Flyer

MSDS - E086 E087 v2

Pro Ligation-Free Cloning Kit - Quick Start Protocol

User Manual

Pro Ligation-Free Cloning Kit

E086/E087 by Applied Biological Materials is the Pro Ligation-Free Cloning Kit. Exonuclease-mediated, ligation-free, restriction-free cloning of PCR fragments into any linearized vector in 15–30 minutes. No T4 ligase or insert restriction digest required. Available as E086 (20 rxn) or E087 (50 rxn). Store at −20°C.

Pro Ligation-Free Cloning Kit (20 reactions)

Pro Ligation-Free Cloning Kit (50 reactions)

<h2 data-section-id="k59sk3" data-start="0" data-end="52">What are transformation kits and competent cells?</h2>
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Transformation kits and competent cells consist of E. coli strains prepared to take up exogenous plasmid DNA, either through chemical competence (calcium chloride treatment, transformed via heat shock) or electrocompetence (transformed via electroporation), along with associated transformation buffers and protocols. Related entities include transformation efficiency (transformants per microgram), heat-shock and electroporation methods, strain genotype (recA, endA deficiency for plasmid stability), and downstream plasmid recovery for sequencing or transfection.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li>Mix &amp; Go! Transformation Kits, rapid protocols for preparation of competent E. coli in&nbsp;</li>
<li>ProClone&trade; Competent Cells with optimized E. coli strains for cloning and library generation.</li>
<li>Specialized Competent E. coli Strains ready-made JM109, DH5 Alpha, and Zymo 10B cells for regular and complex assembly.</li>
<li>Autolysis Competent Cells: designed for simplified cell lysis and streamlined protein expression using XJa and XJb strains</li>
<li>Frozen-EZ Yeast Transformation Kits for genetic manipulation of yeast strains.</li>
</ul>
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<h2 data-section-id="1ld88i5" data-start="812" data-end="876">How to choose transformation kits and competent cell products</h2>
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Chemically competent vs. electrocompetent cells
Chemically competent cells use a heat-shock protocol and are generally easier to handle for routine cloning, while electrocompetent cells require an electroporator but can achieve higher transformation efficiency for large plasmids, low-copy constructs, or DNA with modifications that reduce chemical transformation efficiency.
General cloning strains vs. protein expression strains
General cloning strains are selected for high transformation efficiency and genetic features (such as recA and endA deficiency) that improve plasmid stability and quality for downstream sequencing, while protein expression strains carry features supporting recombinant protein production, such as T7 RNA polymerase expression for IPTG-inducible systems; using a cloning strain for protein expression (or vice versa) generally gives suboptimal results.
Transformation efficiency requirements
Routine cloning of standard-sized plasmids works well with competent cells rated at 10^6&ndash;10^8 transformants per microgram, while ligation reactions with very low DNA concentrations (such as multi-fragment seamless assembly products) may benefit from higher-efficiency cells rated above 10^8 transformants per microgram.
Transformation kits may include a positive control plasmid and competent cells for validating transformation efficiency before using precious experimental DNA; this is particularly useful when troubleshooting low transformation yields to distinguish competent cell quality issues from ligation or assembly reaction issues.
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<h2 data-section-id="1fljvas" data-start="3947" data-end="3972">Specifications context</h2>
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Competent cell transformation efficiency is reported in transformants per microgram of standard control plasmid DNA, with general cloning strains commonly rated at 10^6&ndash;10^8 and high-efficiency formulations exceeding 10^8 transformants per microgram. Cells are typically supplied as frozen aliquots (e.g., 50&ndash;100 microliters per tube) for single-use transformations, shipped on dry ice and stored at -80&deg;C, with repeated freeze-thaw substantially reducing efficiency.&nbsp;
This sub-category is part of MBP's cloning catalog alongside ligation independent cloning kits and chemicals/reagents, the upstream steps of a cloning workflow. Labs producing custom lentivirus for immortalization can also review MBP's cell immortalization catalog. MBP's specialist team can help confirm strain genotype and transformation efficiency requirements for a specific cloning or expression project before order placement.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get high-quality transformation kits and competent cells for your lab.

Transformation Kits & Competetent Cells

MSDS

ProClone™ Competent Cells

E003 by Applied Biological Materials is high-efficiency chemically competent DH5α E. coli cells, 1.2 mL (6 × 200 µL). Transformation efficiency &gt;1 × 106 CFU/µg DNA. endA1 (high-quality plasmid), fhuA2 (T1 phage resistant), recA1 (reduced recombination), blue/white screening capable. Store at −80°C.

Expression System General

<h2>What Are Proteases?</h2>
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Proteases (also called proteinases or peptidases) catalyze the hydrolysis of peptide bonds in proteins and polypeptides. They are classified by their catalytic mechanism into serine proteases (Proteinase K, trypsin, chymotrypsin, subtilisin), cysteine proteases (papain, calpain), aspartic proteases (pepsin), metalloproteases (thermolysin, carboxypeptidase A), and threonine proteases. In research applications, proteases serve roles that span nucleic acid purification, proteomics sample preparation, cell culture maintenance, recombinant protein processing, and structural biology. Trypsin cleaves at the carboxyl side of lysine and arginine residues and generates peptides in the 500-3,000 Da mass range ideal for LC-MS/MS peptide identification. Select a protease based on substrate specificity (broad-spectrum vs. site-specific), compatibility with your lysis or reaction buffer, and the need for downstream inactivation.
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<h2 dir="ltr">What you will find:</h2>
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<a href="https://mbpinc.net/reagents/molecular-biology/enzymes-and-inhibitors/proteases/proteinase-k" title="Proteinase K:">Proteinase K:</a> A robust serine protease (available in lyophilized or liquid formats) designed for rapid protein digestion and the inactivation of nucleases during sensitive nucleic acid isolation.
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Aprotinin, A high-purity competitive inhibitor of serine proteases, is essential for preventing unwanted proteolytic activity during delicate protein purification and extraction steps.
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Protease Inhibitor Cocktail, A pre-formulated, multi-component blend engineered to provide immediate, broad-range protection against a variety of endogenous proteases in cellular lysates.
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TEV Protease, A specialized endopeptidase with ultra-fine specificity, is utilized for the precise, site-directed cleavage of affinity tags from recombinant fusion proteins.
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<h2>How to Choose Proteases</h2>
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Nucleic acid purification
Proteinase K is the first choice for nucleic acid extraction because it is a broad-spectrum serine endopeptidase that digests nucleases (DNases, RNases), histones, and structural proteins in the presence of SDS, urea, EDTA, and most detergents. It is active from pH 4.0 to 12.5 with optimal activity at pH 8.0, and at temperatures from 20 to 60 degrees C. Inactivation at 95 degrees C for 5 minutes or by proteinase K inhibitors (PMSF) is required before downstream enzymatic steps.
Proteomics and mass spectrometry
Trypsin (sequencing grade) is the industry standard for bottom-up proteomics: it generates predictable lysine/arginine-terminated peptides of 6-25 amino acids, well-suited for LC-MS/MS analysis on Orbitrap and Q-TOF platforms. Chymotrypsin (cleaves after Phe, Tyr, Trp, Leu) and Glu-C/V8 protease (cleaves after Glu in phosphate buffer, or Glu+Asp in bicarbonate) are used for orthogonal digestions to improve protein sequence coverage. Lys-C protease (cleaves after Lys only) is stable in 8 M urea and is used for denatured or hard-to-digest proteins.
Cell culture dissociation
Trypsin at 0.25% (w/v) with 0.53 mM EDTA is the standard reagent for detaching adherent mammalian cells from culture surfaces. Recombinant trypsin formulations avoid the lot variability and animal-derived pathogen concerns of porcine pancreatic trypsin. Collagenase (types I, II, III, IV) is used for tissue dissociation, particularly for primary cell isolation from solid tissues, including liver, pancreas, breast, and adipose.
Recombinant protein processing
Site-specific proteases are used to remove N-terminal affinity tags (His6, GST, MBP) from purified recombinant proteins without leaving extra residues. Thrombin cleaves after Arg in the sequence LVPR/GS; Factor Xa cleaves after Arg in IE/DGR; TEV protease cleaves between Gln and Ser in ENLYFQ/S. TEV is the most commonly used because of its stringent 7-amino-acid recognition sequence, which minimizes off-target cleavage.
Buffer compatibility
Confirm protease compatibility with your lysis buffer before use. Proteinase K retains activity in 1% SDS, 4 M urea, 1% Triton X-100, and 10 mM EDTA. Trypsin is inhibited by EDTA (chelates the Ca2+ that stabilizes trypsin structure) and by soybean trypsin inhibitor (SBTI). Chymotrypsin is inhibited by PMSF (an irreversible serine protease inhibitor). Factor Xa is inhibited by benzamidine.
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<h2>Specifications Context</h2>
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Trypsin activity for proteomics is expressed in TAME units (N-alpha-p-tosyl-L-arginine methyl ester esterase units); 1 TAME unit hydrolyzes 1 micromol of TAME per minute at 25 degrees C, pH 8.1. Sequencing-grade trypsin is treated with TPCK (tosylphenylalanyl chloromethyl ketone) to irreversibly inactivate contaminating chymotrypsin activity, which would otherwise generate non-tryptic peptides that complicate database searches. Proteinase K activity is expressed as mAnson units per mL or mg, where 1 mAnson unit releases 1 micromol of tyrosine equivalents per minute from hemoglobin at pH 7.5 and 35.5 degrees C. Single-pot, solid-phase-enhanced sample preparation (SP3) using magnetic bead-bound proteases is increasingly adopted in proteomics to reduce sample loss and processing time for low-input samples.&nbsp;
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Secure your sample stability&mdash;<a href="https://mbpinc.net/contact-us">contact the MBP team</a> today to request a quote for our professional-grade proteases.

Proteases

Protease Inhibitor Cocktail

Protease Inhibitor Cocktail G135 — ready-to-use liquid solution for broad protease inhibition during cell lysis. Available without EDTA (G135) or with EDTA (G135E). Recommended: 1 ml per 100 ml lysate at 10⁸ cells/ml. Store at −20 °C, stable 1 year.

Recommended Protocol

Shipping condition

Protease Inhibition Cocktail with EDTA

<h2>What Is Proteinase K?</h2>
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Proteinase K is a subtilisin-related serine endopeptidase with a molecular weight of 28,930 daltons (28.9 kDa), first isolated from Tritirachium album Limber in 1974, and named for its ability to digest keratin ('K') as its original substrate. The enzyme cleaves peptide bonds at the carboxylic side of aliphatic, aromatic, and basic amino acid residues, making it effective against nearly all protein substrates, including histones, chromatin-bound proteins, cell membrane proteins, and contaminating nucleases (DNases, RNases). Critically, Proteinase K retains full activity in the presence of 1% SDS, 4 M urea, 1% Triton X-100, 0.5% N-lauroylsarcosine, EDTA at 5 mM, and common reducing agents -- conditions under which most other proteases are inactivated. Use Proteinase K in any protocol requiring enzymatic removal of proteins from nucleic acid preparations, including genomic DNA, total RNA, plasmid, phage, and cfDNA extraction from tissues, blood, FFPE samples, bacteria, yeast, and environmental samples.
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<h2 dir="ltr">What you will find:</h2>
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Proteinase K (Lyophilized): A stable, high-purity powder ideal for prolonged storage and tailored buffer preparation.
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Proteinase K Solution: A prepared, stable liquid that reduces preparation time and minimizes pipetting mistakes.
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Proteinase K (G029): A specially refined grade designed for the swift inactivation of RNases and DNases in sensitive procedures.
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<h2>How to Choose Proteinase K</h2>
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Solution vs. lyophilized
Proteinase K is supplied as lyophilized powder (commonly at 30 mAnson units/mg or 20 mg/mL equivalent activity) or as a ready-to-use solution (typically 20 mg/mL in storage buffer). Lyophilized powder offers longer shelf life (up to 2 years at -20 degrees C), cost efficiency for high-volume users, and flexibility in buffer preparation. Solutions are convenient for low-volume use and eliminate weighing errors. Both formats deliver equivalent specific activity when the lot certificate is checked.
Molecular biology grade vs. standard
Molecular biology grade Proteinase K is certified free of detectable endonuclease, exonuclease, DNase, and RNase activities by stringent lot-release assays. Standard grades may carry trace nuclease activity that degrades DNA or RNA during digestion. For any application where nucleic acid integrity matters -- DNA sequencing, NGS library prep, RT-PCR, FISH, or southern blotting -- use only molecular biology grade or equivalent.
Recombinant vs. native
Recombinant Proteinase K (expressed in Pichia pastoris or E. coli) offers freedom from animal-derived material, greater lot-to-lot consistency, and suitability for regulated or GMP-adjacent workflows. Native Proteinase K (isolated from T. album culture) is standard for most research applications. Both must carry DNase/RNase-free certification for nucleic acid use.
Concentration and unit dose
Standard working concentrations are 50-200 microgram/mL for most DNA/RNA extraction protocols in lysis buffer. Higher concentrations of 1-5 mg/mL are used for FFPE tissue deparaffinization and protein digestion, and for prion inactivation studies (where Proteinase K at 10-50 microgram/mL is used to assess proteinase K-resistant PrP accumulation). Proteinase K retains activity for up to 24 months when stored at -20 degrees C in aliquots.
Temperature and pH optimization
Optimal activity is at 20-60 degrees C and pH 7.5-12.0; activity at pH 8.0 and 55 degrees C is used in most nucleic acid extraction protocols. Activity at 37 degrees C is approximately 80% of the 55-60 degrees C optimum. Calcium ions (1 mM Ca2+) stabilize the enzyme structure; EDTA removes calcium and reduces activity, though the enzyme retains sufficient function for nucleic acid prep protocols. Heat-inactivation above 95 degrees C for 5 minutes is complete and does not require chemical inhibitors.
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<h2>Specifications Context</h2>
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Proteinase K specific activity is commonly reported in Anson units (mAnson units/mg): one mAnson unit releases 1 micromol of tyrosine equivalents per minute from hemoglobin at pH 7.5 and 35.5 degrees C. Proteinase K is fully denatured at 95 degrees C in 5 minutes without requiring PMSF or other chemical inhibitors, making heat inactivation the simplest and most reliable option before downstream enzymatic steps such as PCR or reverse transcription. The enzyme is deactivated above 95 degrees C within the first cycle of PCR thermocycling, preventing interference with polymerase activity in protocols that include Proteinase K in the PCR reaction. Proteinase K is a universal reagent in automated nucleic acid extraction platforms, including MagNA Pure, KingFisher, and Hamilton NGS workstations; solution-format Proteinase K is specified in most automated platform kits.&nbsp;
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Simplify your laboratory tasks&mdash;<a href="https://mbpinc.net/contact-us">reach out to the MBP team</a> now for a price estimate on our top-tier Proteinase K products.

Proteinase K

DataSheet

G029 Proteinase K 20 mg/ml by ABM — serine protease for protein digestion and nuclease inactivation in DNA/RNA extraction. 1.0 ml; store at 2–8°C (short term) or −20°C up to 1 year (long term). For genomic DNA, RNA isolation, FFPE extraction, and ChIP.

Concentration

<h2 dir="ltr">What Are Adenoviral Cloning Vectors?</h2>
 An adenoviral shuttle plasmid (transfer vector) contains a transgene expression cassette flanked by adenoviral genomic sequences that mediate recombination into the full adenoviral backbone genome. This shuttle plasmid is the starting point for constructing any custom recombinant adenovirus. Successful adenoviral studies begin with the appropriate cloning backbone. Choose a vector compatible with your development workflow.
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<h2 dir="ltr">What you will find:</h2>
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pShuttle backbone vectors, including pShuttle(+), pShuttle(-), and pShuttle-His variants for gene insertion and adenoviral construct assembly&nbsp;
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Tagged expression vectors such as pShuttle-His-18x and pShuttle-2A-RFP for co-expression and protein labeling studies
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Functional assay and reporter vectors, including pPromoter and pSiShuttle for transcriptional analysis and gene regulation&nbsp;
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Ready-to-use adenoviral DNA for simplified preparation of constructs
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</ul>
<h2 dir="ltr"> How to Choose an Adenoviral Cloning Vector</h2>
 Promoter Selection CMV, EF1&alpha;, and CAG promoter options are available for driving transgene expression &mdash; CMV offers strong, broadly active expression, while EF1&alpha; and CAG can provide more consistent expression in certain cell types. Cloning Method Compatibility Gateway-compatible backbones enable site-specific recombination cloning without restriction enzymes, useful for parallel construct generation. Standard multiple cloning sites (MCS) with conventional restriction sites remain available for traditional cloning workflows. Deletion Configuration E1/E3-deleted Ad5 backbones are the standard configuration &mdash; E1 deletion renders the virus replication-deficient, while E3 deletion provides additional cloning capacity. Downstream Production Pathway The shuttle plasmid recombines into the full adenoviral genome during virus production &mdash; selecting a shuttle vector compatible with your packaging cell line and downstream amplification approach avoids compatibility issues later in the workflow. Reporter/Selection Inclusion Shuttle vectors with fluorescent reporters (GFP) or selection markers built into the cassette simplify confirmation of successful cloning and subsequent virus packaging steps. 
<h2 dir="ltr"> Specifications Context</h2>
 After cloning the transgene into an adenoviral shuttle plasmid, the construct is recombined into the full adenoviral backbone genome in packaging systems or recombination-based workflows, followed by amplification in complementing cell lines to generate recombinant adenoviral stocks. Gateway-compatible shuttle vectors enable transfer of validated entry clones into the adenoviral system without re-cloning. Sequence verification of the shuttle construct by restriction analysis or sequencing is recommended before recombination to ensure construct integrity. Product availability reflects MBP&rsquo;s catalogue as of mid-2026. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP for guidance on shuttle vector selection based on promoter configuration and experimental design requirements.

Cloning Vectors

Selection-Drug Killing Curve

Adenovirus-2-Expression-Protocol

pShuttle(-) Vector

A003 by Applied Biological Materials is a 5.0 µg pShuttle(-) adenoviral shuttle plasmid for the Adeno-1™ Adenovirus Expression System. CMV promoter; in vitro ligation-based adenoviral vector construction. More restriction enzyme sites than competing systems. Not for resale. Store at −20°C or below.

Vector

Note

pShuttle(+) Vector

A002 by Applied Biological Materials is the pShuttle(+) adenoviral shuttle vector (5.0 µg) from the Adeno-1&trade; Adenovirus Expression System with CMV promoter. Clones your gene of interest via in vitro ligation with the adenoviral backbone — no in vivo homologous recombination. More unique restriction enzyme sites than any other adenoviral system. Non-integrating adenoviral transient expression. Store at &minus;20°C.

pShuttle-2A-RFP Vector

A021 by Applied Biological Materials is a pShuttle adenoviral shuttle vector (10 µg) from the Adeno-8&trade; system. Clone your promoter and gene of interest into the MCS; RFP is co-expressed via a T2A self-cleaving peptide as a real-time transduction reporter. For non-integrating adenoviral transgene co-expression with RFP in dividing and non-dividing cells. Store at &minus;20°C.

pShuttle-HA

A009 by Applied Biological Materials is pShuttle-HA — a 5.0 µg Adeno-2™ adenoviral shuttle plasmid with an HA tag and MCS for convenient subcloning. For HA-tagged recombinant adenoviral vector construction with A001 backbone. Facilitates transgene detection and purification by anti-HA antibodies. Store at −20°C or below.

pShuttle-His

A008 by Applied Biological Materials is a 5.0 µg pShuttle-His adenoviral shuttle plasmid for the Adeno-2™ system. His-tagged expression cassette with MCS for transgene subcloning. Enables His tag-based detection (anti-His WB, IF) and IMAC purification of adenovirally expressed proteins. Store at −20°C or below.

pShuttle-His-18x Vector

A020 by Applied Biological Materials is a 10 µg pShuttle-His-18x adenoviral shuttle plasmid (Adeno-2™ system) with an 18× polyhistidine tag. Enhanced Ni-NTA IMAC affinity and anti-His detection sensitivity compared to standard 6× His. MCS for transgene subcloning. Store at −20°C or below.