ABM Reagents

<h2>What are Protein Crosslinking, Labeling, and Modification Reagents?</h2>
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These reagents covalently bond proteins together or attach functional labels to proteins for downstream analysis. They are broadly divided into homobifunctional and heterobifunctional crosslinkers based on the type of reactive groups they contain.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" aria-level="1">
Ni-IDA Agarose Resins: High-capacity purification systems (G250) utilizing immobilized Ni2+ cations and iminodiacetic acid groups for the rapid, one-step isolation of His-tagged proteins under both native and denaturing conditions.
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<li dir="ltr" aria-level="1">
Versatile Purification Formats: Ready-to-use resins suitable for batch or gravity-flow chromatography, offering an economical and reusable solution for antibody purification and the study of protein-DNA interactions at any scale.
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</ul>
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<h2>How to Choose Crosslinking and Labeling Reagents</h2>
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Decide Between Homobifunctional and Heterobifunctional Chemistry
Homobifunctional crosslinkers contain identical reactive groups at both ends and are used to stabilize or capture existing protein interactions. Heterobifunctional crosslinkers contain two different reactive groups, allowing controlled stepwise conjugation between specific molecules.
Choose Water-Soluble Crosslinkers for Aqueous or Cellular Work
BS3 is the water-soluble form of DSS and is preferred for reactions in aqueous buffers or intact cell systems while maintaining the same chemistry and spacer length.
Match Spacer Arm Length to Molecular Distance
The spacer arm determines the maximum distance between two reactive groups that can be linked. Incorrect spacing can reduce efficiency or increase non-specific crosslinking.
Use Heterobifunctional Chemistry for Defined Conjugates
Sulfo-SMCC enables sequential coupling of amine-containing molecules with sulfhydryl-containing partners, commonly used for antibody-enzyme or antibody-carrier conjugation workflows.
Consider Cleavable Crosslinkers for Reversible Studies
Cleavable crosslinkers such as DTSSP contain disulfide bonds that can be reduced later, enabling separation of linked proteins for downstream analysis such as mass spectrometry.
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<h2>Specifications Context</h2>
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DSS has an 11.4 &Aring; spacer arm that defines the maximum distance between linked amine groups, while BS3 provides the same chemistry with improved water solubility for aqueous applications. Cleavable crosslinkers are increasingly used in mass spectrometry workflows for mapping protein-protein interactions.
Streamline your protein modification workflows&mdash;<a href="https://mbpinc.net/contact-us">contact the MBP team</a> today for a quote on our professional Ni-IDA resin solutions.

Crosslinking, Labeling and Protein Modification

Ni-IDA Agarose Resins

Ni-IDA Agarose Resins (G250, G251, G253) by Applied Biological Materials are nickel-charged IDA agarose resins for IMAC purification of 6×His- and 10×His-tagged recombinant proteins under native or denaturing conditions. Available in multiple pack sizes.

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<h2>What are L-glutamine and amino acid supplements?</h2>
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L-glutamine and amino acid supplements are cell culture additives used to support cellular metabolism, growth, viability, and biosynthesis. They provide nutrients that may become depleted during culture or are required in greater quantities than those supplied by basal media alone. These supplements are commonly added to media such as DMEM, RPMI 1640, MEM, and DMEM/F-12 to optimize cell growth and maintain healthy cultures.
L-glutamine is one of the most important supplements in mammalian cell culture because it serves as a major energy source and is required for protein synthesis, nucleotide production, and glutathione synthesis. However, because free L-glutamine is unstable in solution and gradually breaks down into ammonia and pyroglutamate, many laboratories use stabilized alternatives such as GlutaMAX (L-alanyl-L-glutamine) for extended culture applications.
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<h2>What you will find:</h2>
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<ul>
<li>L-Glutamine stable formulations for sourcing of essential nitrogen</li>
<li>Non-Essential Amino Acids (NEAA) for growth media enhancement</li>
<li>Synthetic Polymer Coatings Poly-L-Ornithine and Poly-L-Lysine solutions for better cell attachment.</li>
<li>Extracellular Matrix Proteins used for cell adhesion and include high-purity Fibronectin and Vitronectin coating reagents</li>
<li>Adhesion Promotion Reagents for improving the surface properties of culture vessels.</li>
</ul>
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<h2>How to choose L-glutamine and amino acid supplements</h2>
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L-glutamine concentration and format
Most mammalian cell culture systems use L-glutamine concentrations between 2&ndash;6 mM, although requirements vary depending on the medium and cell type. It is commonly supplied as:
<ul>
<li>USP-grade powder for custom media preparation</li>
<li>200 mM liquid stock solutions for direct supplementation</li>
</ul>
Liquid stocks should be aliquoted and stored frozen to minimize repeated freeze-thaw cycles and preserve stability.
Understanding glutamine degradation
Free L-glutamine gradually breaks down in aqueous solution through a non-enzymatic process that produces ammonia and pyroglutamate. This degradation accelerates with increasing temperature, storage time, and exposure to common cell culture buffer systems.
Ammonia accumulation can negatively affect cell growth, viability, productivity, and experimental reproducibility, particularly during long culture periods without media replacement.
GlutaMAX and stable glutamine alternatives
GlutaMAX (L-alanyl-L-glutamine) is a stabilized dipeptide form of glutamine designed to resist degradation in culture media. Once taken up by cells, it is converted into usable glutamine while maintaining greater stability during incubation.
<ul>
<li>Reduced ammonia accumulation</li>
<li>Improved media stability</li>
<li>Better performance in long-term cultures</li>
<li>Less frequent media replacement requirements</li>
</ul>
For most applications, GlutaMAX can directly replace standard L-glutamine without protocol modifications.
MEM non-essential amino acids (NEAA)
NEAA supplements provide amino acids that cells can synthesize themselves but may benefit from receiving directly from the culture medium. Supplementation reduces the metabolic burden of amino acid production and often improves growth rates and cell viability.
NEAA solutions are typically supplied as 100X concentrates and diluted to 1X working concentration.
HT supplement
HT supplement contains hypoxanthine and thymidine and is commonly used following HAT selection during hybridoma production. It supports nucleotide synthesis through salvage pathways while cells recover from selection pressure.
MEM vitamin solution
Vitamin supplements provide additional growth-supporting micronutrients that may become limiting during prolonged culture or in demanding applications. These supplements are often used alongside amino acid supplementation to enhance culture performance.
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<h2>Specifications context</h2>
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The standard L-glutamine concentration used in many mammalian culture systems is approximately 2 mM, though actual requirements vary by medium formulation and cell type. Free L-glutamine remains stable as a dry powder and frozen stock solution but degrades progressively once added to liquid media.
Stabilized glutamine products such as GlutaMAX have become increasingly common because they maintain glutamine availability while minimizing ammonia accumulation during incubation. These formulations are particularly valuable in long-duration experiments and high-density culture systems.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today to get research-grade supplements for your lab.

L-Glutamine & Amino Acids

Non-Essential Amino Acids (100X)

Non-Essential Amino Acids (100X) TM068 (ABM) — 100 ml sterile 100× NEAA solution. Dilute 1:100 in culture medium for 1× working concentration. Reduces metabolic burden in mammalian cell culture, serum-free media, and iPSC/ESC applications. Sterility tested, QC validated. Store 2–8 °C, protect from light. RUO.

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<h2 dir="ltr"> What is a dNTP Mix?</h2>
 A dNTP Mix is a premixed solution containing all four deoxynucleotide triphosphates - dATP, dCTP, dGTP, and dTTP - combined in equimolar amounts at a standard working concentration, commonly 10 mM each (40 mM total) when prepared from 100 mM individual stocks. The premix is ready for direct addition to a PCR, qPCR, or cDNA synthesis reaction without further dilution or balancing.&nbsp; Choose a premixed dNTP Mix for routine PCR setup where convenience and lot-to-lot consistency outweigh the need for custom nucleotide ratios.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li>High-purity nucleotide mixes for PCR and DNA synthesis applications, including PureStrand&trade; Ultra-Pure dNTP Mix (Azura)</li>
<li>Standard 10 mM each dNTP formulations suitable for routine PCR workflows, including dNTP Mix (10 mM each) formats</li>
<li>Higher-concentration nucleotide solutions, such as Accuris 40 mM dNTP Mix for optimized reaction setup flexibility</li>
<li>Ready-to-use balanced dNTP blends supporting DNA polymerase activity in PCR and related enzymatic amplification reactions</li>
<li>Nucleotide triphosphate formulations, including NTP Mix (G945), for broader enzymatic and molecular biology applications</li>
</ul>
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<h2>How to Choose a dNTP Mix</h2>
 Concentration Format Common premix formats include 10 mM each (40 mM total, a 50x working stock relative to the 0.2 mM final concentration) and 2.5 mM each (10 mM total, a 10x working stock). Select the format that aligns with your typical reaction volume and pipetting precision - more dilute stocks reduce pipetting error for small-volume reactions. Equimolar Accuracy Because all four nucleotides are pre-combined at equal concentrations, a premixed dNTP Mix removes the risk of manual equimolar-mixing error that can occur when combining four separate 100 mM stocks - unbalanced ratios increase misincorporation frequency during polymerisation. Throughput Considerations For high-throughput PCR setups across multi-well plates, a premixed dNTP Mix reduces the number of individual pipetting steps per reaction compared to adding four separate dNTP stocks, improving both speed and reproducibility. Compatibility with Master Mixes Many 2x PCR master mixes already include dNTPs at optimised concentrations - a separate dNTP Mix is primarily needed for labs assembling reactions from individual components (enzyme, buffer, dNTPs, primers) rather than using a pre-formulated master mix. Application Range A standard equimolar dNTP Mix supports PCR, qPCR, and cDNA synthesis (as substrate for reverse transcriptase). Confirm the concentration and purity grade align with the specific requirements of high-fidelity or long-range PCR, if applicable. 
<h2 dir="ltr"> Specifications Context</h2>
 To prepare a standard PCR reaction at the recommended 0.2 mM final concentration of each dNTP using a 10 mM-each premix, 1 &micro;L of the premix is added per 50 &micro;L reaction. Equal nucleotide concentrations in the premix are essential because unbalanced dNTP ratios increase the frequency of misincorporation during DNA synthesis - a factory-balanced premix removes this variable. dNTP Mix is typically supplied in water at pH 7.5, stable at &minus;20 &deg;C with documented multiple freeze-thaw tolerance; thawing and mixing thoroughly before use is recommended, especially for higher-concentration formats. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get high-purity dNTP mixes tailored for your laboratory workflows.

dNTPs Mix

DataSheet

MSDS

NTP Mix

G945 NTP Mix by ABM — pre-mixed ATP, CTP, GTP, UTP (25 mM each) in 1.0 ml; &gt;99% HPLC purity; IVT-tested; stable 2 years at −20°C; for SP6, T3, T7 IVT reactions. Simplifies NTP addition vs. individual NTP Set (G946). Shipped on Ice Pack.

Product Features

<h2 dir="ltr">What is a dNTP Set?</h2>
 A dNTP Set is a complete set of individual, highly pure deoxynucleotide triphosphates - dATP, dCTP, dGTP, and dTTP - each supplied as a separate 100 mM solution in water. Each nucleotide can be diluted and mixed with any of the other three to the desired final concentration or ratio, giving researchers full control over custom PCR, qPCR, or labelling mix preparation. Choose a dNTP Set when you need to prepare custom-concentration mixes, run multiple applications with different nucleotide requirements, or want individual stocks for non-equimolar formulations.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li style="color: #000000;" data-section-id="rmv9gj" data-start="173" data-end="310">Pre-mixed deoxynucleotide triphosphate (dNTP) sets for PCR and DNA synthesis applications, including PureStrand&trade; dNTP Set (Azura)</li>
<li style="color: #000000;" data-section-id="1u2l5t9" data-start="311" data-end="437">High-concentration nucleotide formulations such as Accuris 100mM dNTP Set for flexible reaction setup and optimization</li>
<li style="color: #000000;" data-section-id="kkyf1s" data-start="438" data-end="533">Standard dNTP set formats suitable for routine PCR workflows, including dNTP Set (G050)</li>
<li style="color: #000000;" data-section-id="1j5qt5c" data-start="534" data-end="653">Nucleotide triphosphate formulations for enzymatic and molecular biology applications, including NTP Set (G946)</li>
<li style="color: #000000;" data-section-id="1nhlqes" data-start="654" data-end="773">Ready-to-use balanced nucleotide sets supporting DNA polymerase activity in PCR and related amplification reactions</li>
</ul>
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<h2 dir="ltr">How to Choose a dNTP Set</h2>
 Custom Mix Preparation A dNTP Set's primary advantage is flexibility - preparing a working mix at any desired total concentration and ratio, including non-standard formulations for specific applications such as nucleotide analog substitution (e.g., dUTP in place of dTTP for anti-carryover-contamination strategies). Multiple Application Support Labs running PCR, qPCR, cDNA synthesis, and DNA labelling from the same stock benefit from a single dNTP Set, since each application may call for slightly different concentrations or ratios prepared from the same individual 100 mM tubes. Stock Concentration 100 mM is the standard individual stock concentration for dNTP Sets, providing substantial dilution headroom - a 100 mM stock can be diluted 1000-fold to reach a 0.1 mM working concentration, or combined at lower dilution ratios for higher-concentration premixes. Equimolar Mixing Discipline When preparing a standard equimolar mix from a dNTP Set, equal volumes of each 100 mM nucleotide are combined with water to the desired total concentration - for example, equal-volume mixing of all four 100 mM stocks yields a 25 mM-each premix at 100 mM total. Purity and Lot Testing PCR-grade dNTP Sets are typically tested at greater than 99% purity by HPLC, with concentration verified by optical density spectrometry and absence of human/E. coli DNA confirmed by qPCR - important documentation for labs requiring lot-specific quality records. 
<h2 dir="ltr"> Specifications Context</h2>
 To prepare a standard equimolar working mix from a dNTP Set, combining equal volumes of each 100 mM individual stock yields a mix at 25 mM of each nucleotide (100 mM total); this can be further diluted to common working stocks such as 10 mM each (a 50x stock relative to the 0.2 mM PCR final concentration) or 2.5 mM each (a 10x stock). The performance of each lot is typically functionally tested in a PCR requiring all four nucleotides, with concentration verified by optical density spectrometry and purity confirmed by HPLC. dNTP Sets are stable at &minus;20 &deg;C with documented multiple freeze-thaw tolerance; thawing and mixing each individual tube thoroughly before use is recommended. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get reliable dNTP sets for your next successful experiment.

dNTP Set

NTP Set

G946 NTP Set by ABM — 4×250 µl of ATP, CTP, GTP, and UTP (100 mM each); &gt;99% HPLC purity; functionally tested; stable 2 years at −20°C; for in vitro transcription with SP6, T3, and T7 RNA polymerases. Shipped on Ice Pack.

<h2 dir="ltr">What Are Organelle Labeling Lentiviral Particles?</h2>
 Organelle labeling lentiviral particles are ready-to-transduce viral stocks that deliver a fluorescent protein (GFP or RFP) fused to a validated subcellular targeting sequence. After stable integration, transduced cells express a fluorescent marker localised to a specific organelle &mdash; providing a heritable, non-toxic alternative to chemical dyes for subcellular imaging. Cellular imaging studies depend on accurate organelle targeting. Choose a labeling system that supports clear visualization and analysis.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Mitochondria labeling viruses for monitoring cellular metabolism and energy dynamics in live-cell studies</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Nucleus labeling viruses for gene expression analysis and nuclear localization tracking in mammalian cells</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Endoplasmic reticulum labeling viruses for studying folding, synthesis, and intracellular transport pathways of proteins</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Golgi apparatus labeling viruses for protein processing and for vesicle trafficking visualization</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Plasma membrane labeling of viruses for cell interaction studies, receptor localization, and membrane dynamics</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Lysosome labeling viruses for autophagy and degradation pathway-related applications</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Peroxisome labeling viruses for oxidative stress response and metabolic studies</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Cytoskeleton labeling viruses for cell morphology analysis and structural organization studies</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Autophagosome labeling of viruses for cellular recycling pathway and autophagy flux studies</li>
</ul>
<h2 dir="ltr"> How to Choose an Organelle Labeling Lentivirus</h2>
 Target Organelle Available targeting constructs cover mitochondria, endoplasmic reticulum, nucleus, Golgi apparatus, lysosome, peroxisome, plasma membrane, autophagosome, and cytoskeleton &mdash; select based on the structure of interest in your experiment. Fluorophore Choice GFP- and RFP-based constructs allow flexibility for multiplexed imaging &mdash; pairing an organelle marker with a second fluorescent reporter (e.g. a protein-of-interest fusion) in the red or green channel respectively. Stable vs. Transient Labeling Needs Lentiviral delivery produces stable, integrated reporter expression maintained across cell divisions &mdash; essential for long-term time-lapse imaging, drug treatment time courses, or generation of reporter cell line banks. Cytotoxicity Considerations Unlike chemical dyes (MitoTracker, LysoTracker, ER-Tracker), which can be cytotoxic at working concentrations and require repeated application, lentiviral organelle markers avoid repeated dye loading and chronic toxicity in long-term experiments. Multiplexing Strategy When combining an organelle marker with other fluorescent reporters, select spectrally distinct fluorophores to avoid channel overlap in fluorescence microscopy or flow cytometry. 
<h2 dir="ltr"> Specifications Context</h2>
 Organelle labeling lentiviral particles sourced from Applied Biological Materials (ABM) are supplied as ready-to-transduce stocks, eliminating the need for in-house viral production. Stable genomic integration enables longitudinal analysis of organelle morphology and dynamics across cell passages, treatments, and differentiation time courses. All lentiviral workflows require institutional biosafety committee (IBC) approval, with BSL-2 containment standard for third-generation systems. Product availability reflects MBP&rsquo;s catalogue as of mid-2026. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>of MBP to confirm availability for specific organelle targets and fluorophore combinations.

Organelle Labeling Viruses

Important Considerations for Lentiviruses

Nucleus Labeling Lentivirus (LMNB1)(GFP)

LVP706 Nucleus Labeling Lentivirus (LMNB1)(GFP) by Applied Biological Materials delivers Lamin B1-GFP for stable green fluorescent labeling of the nuclear envelope in transduced mammalian cells. Compatible with FITC/488 nm filter sets. RFP version available as LVP707. Store at &minus;80&deg;C.

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Titer	

Nucleus Labeling Lentivirus (LMNB1)(RFP)

LVP707 Nucleus Labeling Lentivirus (LMNB1)(RFP) by Applied Biological Materials delivers Lamin B1-RFP for stable red fluorescent labeling of the nuclear envelope in transduced mammalian cells. Compatible with TRITC/561 nm filter sets; spectrally compatible with GFP-based organelle markers for dual-color imaging. Store at &minus;80&deg;C.

<h2 dir="ltr">What Are Specialized Cloning Vectors &amp; Viral Tools?</h2>
 Standard lentiviral and retroviral expression vectors meet the needs of most overexpression and knockdown workflows, but many cell biology and translational research questions demand purpose-engineered constructs with specialised functions &mdash; subcellular localisation, multi-gene co-expression, or conditional activation. Select the format best suited to your experimental requirements.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/dna/cloning-services-products/specialized-cloning-vectors/-virus/organelle-labeling-viruses">Organelle Labeling Viruses: </a>for live-cell visualization and functional pathway analysis of cellular structures in mammalian systems
</li>
<li dir="ltr" aria-level="1">
Targeted fluorescent labeling of specific cellular structures using viral delivery.
</li>
<li dir="ltr" aria-level="1">
Stable expression of organelle-specific markers for long-term live-cell imaging.
</li>
<li dir="ltr" aria-level="1">
Compatible with fluorescence microscopy for dynamic cellular imaging applications.
</li>
<li data-section-id="8k7pm2" data-start="506" data-end="603" data-is-last-node="">Suitable for studying organelle organization, transport, and cellular function in living cells.</li>
</ul>
<h2 dir="ltr"> How to Choose Specialized Cloning Vectors</h2>
 Subcellular Targeting Needs Organelle-targeted fluorescent labeling lentiviral particles deliver fluorescent protein constructs targeting mitochondria, endoplasmic reticulum, nucleus, Golgi, lysosome, peroxisome, plasma membrane, autophagosome, and cytoskeleton &mdash; providing a stable alternative to chemical dyes for live-cell imaging. Multi-Gene Co-Expression Bicistronic vectors using IRES or 2A self-cleaving peptide sequences enable co-expression of two genes (e.g., a gene of interest and a fluorescent marker) from a single transcript or vector. Conditional Expression Requirements Conditional DIO/FLEX vectors restrict transgene expression to Cre-expressing cells, essential for circuit-specific neuroscience research and in vivo conditional gene expression studies. Temporal Control Needs Inducible constructs (Tet-On/Tet-Off systems) provide doxycycline-regulated transgene activation for experiments requiring temporal control over expression timing. Delivery Format Most specialised constructs are available as lentiviral particles for stable, long-term expression in both dividing and non-dividing cell types, including primary cells and in vivo models. 
<h2 dir="ltr"> Specifications Context</h2>
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Traditional subcellular organelle imaging relies on chemical dyes (e.g., MitoTracker, LysoTracker, ER-Tracker) or antibody-based immunofluorescence in fixed cells; however, chemical dyes are transient and may exhibit cytotoxicity at working concentrations, while antibody-based methods are limited to endpoint imaging. Viral organelle-targeted fluorescent expression systems enable stable, genetically encoded labeling for long-term live-cell imaging and dynamic subcellular tracking. These constructs are supplied in plasmid DNA and pre-packaged viral formats and support applications including organelle dynamics, trafficking studies, and multicolor imaging workflows. Specialized cloning vectors and viral tools are sourced from established manufacturers, including Applied Biological Materials (ABM), and product availability reflects MBP&rsquo;s catalogue as of mid-2026.
&nbsp;
<a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact the MBP team </a>today and get specialized cloning vectors for your lab.

Specialized Cloning Vectors/ Viruses

Specialized Cloning Vectors/ Virus

Oct4 ECFP Reporter Adenovirus

000776A by Applied Biological Materials is the Stemporter™ iPSC Reporter adenovirus (1.0 ml in DMEM/2.5% glycerol) expressing ECFP under an Oct4 promoter for non-integrating, transient identification of pluripotent iPSC clones by cyan fluorescence during reprogramming. ECFP excitation ~434 nm, emission ~477 nm. Non-destructive, real-time pluripotency readout.

<h2 dir="ltr"> What Are Miscellaneous PCR Items?</h2>
 Miscellaneous Items groups supporting reagents used alongside core PCR enzymes, master mixes, and nucleotide components, covering essential but non-core materials required for PCR and RT-PCR workflows. This includes nuclease-free water for reaction setup and dilution, Random Primer (6N) and Oligo(dT)18 primers for cDNA synthesis, and ionic solutions such as MgCl₂, MgSO₄, NaCl, and MnCl₂ used for reaction optimisation. The category also includes certified nuclease-free water options from suppliers such as BIOLOGIX for sensitive molecular biology applications. Use this category to source essential supporting reagents that complete PCR-based experimental workflows alongside primary amplification products. 
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<h2 data-start="432" data-end="459">What you will find:</h2>
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<ul data-start="461" data-end="1220">
<li data-section-id="b5abnd" data-start="461" data-end="635">Nuclease-Free Water: DNase/RNase-free water suitable for PCR, RT-PCR, qPCR, and nucleic acid preparation workflows, including certified molecular biology-grade options.</li>
<li data-section-id="n5mfl0" data-start="636" data-end="789">Primers &amp; Oligos: Random Primer (6N) and Oligo(dT)18 formats for reverse transcription, cDNA synthesis, and related molecular biology applications.</li>
<li data-section-id="1x29qzm" data-start="790" data-end="932">Reaction Components: Molecular biology-grade MgCl₂, MgSO₄, MnCl₂, and NaCl solutions for reaction preparation and protocol optimisation.</li>
<li data-section-id="16hzrat" data-start="933" data-end="1075">Workflow Support Reagents: Essential supporting materials used alongside PCR enzymes, master mixes, and nucleic acid amplification kits.</li>
<li data-section-id="4t6tsy" data-start="1076" data-end="1220">Research-Ready Formats: Convenient reagent sizes suitable for routine academic, biotechnology, and molecular biology laboratory workflows.</li>
</ul>
<h2 dir="ltr"> How to Choose Miscellaneous PCR Items</h2>
 Gel Loading Dyes Loading dyes add density and colour to PCR products for gel electrophoresis, allowing visual tracking of sample migration. Some 2x master mixes include loading dye pre-formulated, but a separate loading dye is needed when using dye-free master mixes or individual-component PCR setups. PCR Enhancers and Additives Additives such as betaine, DMSO, or proprietary enhancer formulations can improve amplification of GC-rich or otherwise difficult templates by reducing secondary structure or improving primer annealing specificity -- useful as a troubleshooting tool when a standard reaction underperforms on a specific target. Nuclease-Free Water Grade Nuclease-free water is used for reaction dilutions, resuspending primers and probes, and as a no-template control in PCR and qPCR setups. Confirm the water is certified free of detectable RNase and DNase activity, particularly for RNA-handling workflows. PCR Tube and Plate Format PCR tubes, strips, and plates are available in formats matching common thermal cycler block configurations -- 0.2 mL individual tubes, 8-well or 12-well strips, and 96-well plates -- select the format matching your thermal cycler and throughput needs. Compatibility with Primary Reagents When ordering supporting consumables alongside primary PCR reagents, confirming tube/plate compatibility with both your thermal cycler and any downstream automation (liquid handlers, plate readers) avoids workflow bottlenecks from mismatched formats. 
<h2 dir="ltr"> Specifications Context</h2>
&nbsp;
Nuclease-free water used in molecular biology workflows is typically certified through DNase/RNase activity testing and supplied in formats suitable for routine PCR, RT-PCR, and nucleic acid dilution applications. Random Primer (6N) and Oligo(dT)18 primers are used in reverse transcription workflows to support cDNA synthesis from RNA templates. Ionic solutions such as MgCl₂, MgSO₄, NaCl, and MnCl₂ are used to support and optimise polymerase activity under different reaction conditions. Most items in this category are stable under standard laboratory storage conditions, with product-specific handling and storage requirements provided by the manufacturer.
&nbsp;
Ensure your experimental stability&mdash;<a href="https://mbpinc.net/contact-us" style="color: #236fa1;">reach out to the MBP team</a> now for a quote on our expert PCR accessories

Miscellaneous Items

Miscellaneous items

Oligo(dT)18 Primer

Oligo(dT)18 Primer G140 — 10 µM, 500 µl in ddH₂O. Universal poly-A mRNA primer for reverse transcription cDNA synthesis at 0.5–1 µM final concentration. For RT-PCR, RT-qPCR, and cDNA library construction from poly-A mRNA. Store −20 °C, avoid freeze–thaw; stable up to 60 months.

Recommended Protocol

<h2 dir="ltr"> What is a cDNA Synthesis Kit?</h2>
 A cDNA synthesis kit provides the complete set of reagents - reverse transcriptase enzyme, reaction buffer, dNTPs, RNase inhibitor, and primers - needed to perform first-strand reverse transcription, converting an RNA sample into a cDNA pool. Unlike one-step RT-PCR kits, which combine RT and PCR in a single reaction with gene-specific primers, a cDNA synthesis kit performs reverse transcription as a standalone step, producing a cDNA template that can be used across multiple subsequent PCR or qPCR reactions targeting different genes. Choose a cDNA synthesis kit when one RNA sample needs to be analysed for multiple gene targets, or when downstream qPCR requires a separately-generated, quality-checked cDNA template. 
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="1o7fvd2" data-start="74" data-end="228">First-strand cDNA synthesis kits for RNA-to-cDNA conversion in gene expression workflows, including AzuraQuant&trade; and AzuraFlex&trade; cDNA synthesis kits</li>
<li data-section-id="z40sk0" data-start="229" data-end="395">Standard cDNA synthesis kits for routine transcriptional analysis and downstream PCR applications, including Accuris qMAX and 1st cDNA Synthesis Kit (FS01-00)</li>
<li data-section-id="1yvybqf" data-start="396" data-end="521">Flexible and high-efficiency cDNA synthesis systems designed to support a range of RNA inputs and experimental conditions</li>
<li data-section-id="sl2teb" data-start="522" data-end="599">miRNA-specific cDNA synthesis solutions for small RNA expression analysis</li>
<li data-section-id="17gz9kt" data-start="600" data-end="770" data-is-last-node="">One-step and integrated RT-based systems and master mixes supporting streamlined reverse transcription workflows, including OneScript Plus and OneScript Hot RT kits</li>
</ul>
<h2 dir="ltr"> How to Choose a cDNA Synthesis Kit</h2>
 Priming Strategy Oligo(dT) primers target the poly-A tail of mRNA, enriching specifically for mRNA-derived cDNA and reducing genomic DNA or rRNA background. Random primers (commonly hexamers) prime throughout the RNA template regardless of sequence, useful for templates lacking a poly-A tail (such as bacterial RNA) or when full-length cDNA coverage, including 5' regions, is needed. Some kits offer a blend of both primer types for balanced coverage. Reverse Transcriptase Included Kits built around M-MLV RNase H-minus reverse transcriptase favour longer, more complete cDNA products, while kits using enhanced-thermostability or inhibitor-resistant reverse transcriptases may better handle GC-rich templates or minimally purified RNA samples. Genomic DNA Elimination Step Some cDNA synthesis kits include a separate genomic DNA elimination (gDNA wipeout) step performed before reverse transcription -- important for accurate gene expression quantification, since residual genomic DNA can contribute false signal in downstream qPCR if primers span regions present in both genomic DNA and the cDNA target. Reaction Volume and Throughput cDNA synthesis kits are available in formats scaled for individual reactions through to high-throughput 96-well formats, allowing labs to match kit format to typical sample batch sizes without excessive reagent waste. Downstream Application Compatibility The resulting cDNA pool should be compatible with the intended downstream application -- standard PCR, qPCR with SYBR Green or probe-based detection, or digital PCR -- confirm that the kit's buffer system does not interfere with the chosen downstream detection chemistry. 
<h2 dir="ltr"> Specifications Context</h2>
 In a two-step workflow, the first step synthesises first-strand cDNA using a reverse transcriptase such as M-MLV-RT with the chosen priming strategy, and the resulting cDNA pool can then be used in multiple separate PCR or qPCR reactions targeting different genes - a key advantage when a single RNA sample needs to be analysed for several targets, since the cDNA synthesis step is performed only once. Genomic DNA elimination steps, where included, are typically performed as a brief pre-treatment with a DNase before the reverse transcription reaction itself begins. cDNA synthesis kit components - enzyme, buffer, dNTPs, and RNase inhibitor - are typically stored at &minus;20 &deg;C; RNase-free water and RNase inhibitor handling throughout the workflow are recommended to prevent RNA template degradation before synthesis.
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get high-precision cDNA synthesis kits for your lab.

cDNA Synthesis Kits

Product Literature

PCR Product Guide

Product Stability Report

OneScript Hot 5X MasterMix

G590 OneScript&reg; Hot 5X RT MasterMix by Applied Biological Materials is a complete, ready-to-use 5X reverse transcription master mix containing OneScript&reg; Hot thermostable RT (RNase H-deficient, active at 60&ndash;72&deg;C), RNaseOFF inhibitor, dNTPs, and mixed primers for high-yield cDNA synthesis from challenging RNA. ISO 13485:2016 MDSAP certified; 100 rxn (400 µl); store at &minus;20&deg;C.

Concentration

<h2>What Is Reverse Transcriptase?</h2>
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Reverse transcriptase is an RNA-dependent DNA polymerase that synthesizes a complementary DNA (cDNA) strand using an RNA template, providing the essential first step for RT-PCR, RT-qPCR, cDNA library construction, and gene expression analysis. Reverse transcriptases differ in thermostability, processivity, RNase H activity, and compatibility with challenging RNA templates. Selecting the appropriate enzyme depends on your RNA quality, transcript length, and downstream application.
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<h2>What you will find:</h2>
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<ul>
<li>Reverse transcriptase-based systems designed to support cDNA synthesis across a range of RNA templates, including workflows involving structured or challenging RNA.</li>
<li>One-step RT-PCR and RT-qPCR kits featuring enzyme systems designed to reduce non-specific amplification through optimized reaction setup and enzyme activation strategies.</li>
<li>High-efficiency reverse transcription and RT-PCR chemistries suitable for low-abundance RNA inputs and sensitive gene expression analysis.</li>
<li>cDNA synthesis and RT-PCR systems supporting a range of transcript lengths depending on enzyme selection and reaction conditions.</li>
<li>Thermostable and high-performance reverse transcription systems designed to improve cDNA yield and workflow reliability across RNA applications.</li>
</ul>
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<h2>How to Choose a Reverse Transcriptase</h2>
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Enzyme Selection
Reverse transcriptases differ in thermostability, RNase H activity, processivity, and compatibility with different RNA templates. Select an enzyme that matches your RNA quality, transcript length, and downstream application requirements.
RNase H Activity
Reverse transcriptases with reduced RNase H activity minimize RNA template degradation during cDNA synthesis, helping improve the yield of longer cDNA products for applications such as full-length transcript analysis and cDNA library preparation.
Thermostability
Thermostable reverse transcriptases can operate at higher reaction temperatures, helping reduce RNA secondary structure and improving cDNA synthesis from GC-rich or highly structured RNA templates.
Template Length and Application
Choose an enzyme validated for your intended transcript length and downstream workflow, whether performing routine RT-PCR, quantitative RT-qPCR, full-length cDNA synthesis, or library preparation.
Workflow Compatibility
Consider whether a stand-alone reverse transcriptase or an integrated one-step RT-PCR system best fits your workflow, throughput requirements, and downstream amplification strategy.
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<h2>Specifications Context</h2>
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Reverse transcriptase enzymes catalyze the synthesis of complementary DNA (cDNA) from RNA templates, enabling downstream PCR- and qPCR-based analysis of gene expression. Modern reverse transcriptases are available with varying levels of thermostability, processivity, and RNase H activity to support applications ranging from routine first-strand cDNA synthesis to amplification of structured or low-abundance RNA targets. Most enzymes are supplied for storage at &minus;20&nbsp;&deg;C, and RNase-free handling practices are recommended throughout RNA workflows to preserve sample integrity and maximize cDNA yield.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get research-grade reverse transcription enzymes for your lab.

Reverse Transcriptase

OneScript Hot Reverse Transcriptase

G593 OneScript&reg; Hot Reverse Transcriptase by Applied Biological Materials is a thermostable (60&ndash;72&deg;C), RNase H-deficient M-MLV RT derivative for high-yield cDNA synthesis from difficult, low-abundance, or partially degraded RNA, formulated with oxidation-resistant RNaseOFF inhibitor. ISO 13485:2016 MDSAP certified; 100 rxn; store at &minus;20&deg;C.

OneScript Hot RT cDNA Synthesis Kit

G594 OneScript&reg; Hot cDNA Synthesis Kit by Applied Biological Materials is a complete 100 rxn kit for cDNA synthesis at 60&ndash;72&deg;C using thermostable, RNase H-deficient OneScript&reg; Hot RT with RNaseOFF inhibitor, dNTPs, Oligo(dT), and Random Primers. The first kit optimized for 60&deg;C cDNA synthesis. ISO 13485:2016 MDSAP certified; store at &minus;20&deg;C.

OneScript Plus cDNA Synthesis Kit

G236 by Applied Biological Materials is the OneScript&reg; Plus cDNA Synthesis Kit (100 rxn) built on an engineered RNase H-null, thermally stable M-MLV RT. Synthesizes cDNA up to 12 kb at 50–55°C, with RNaseOFF inhibitor stable at &lt;1 mM DTT. Three primer options (oligo(dT), random, gene-specific). ISO 13485:2016 MDSAP Certified. Equivalent to G236EU. Store at &minus;20°C.